Restriction map for
pFN21AA0369
(Circular) (Six-base) MAPSORT of: pFN21AA0369.seq Check: 9283 from: 1 to: 6892
pFN21AA0369 6892 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3713 3808 3713
Size: 95 6797
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4702 278
Size: 53 83 186 936 3166 2468
Fragments arranged by size:
3166 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3624 4235 1937
Size: 1687 611 4594
Fragments arranged by size:
4594 1687 611
Acc65I G'GTAC_C
Cuts at: 4213 4213
Size: 6892
AclI AA'CG_TT
Cuts at: 1563 5019 5392 1563
Size: 3456 373 3063
Fragments arranged by size:
3456 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2508 2723 3611 4955 6339 6607 1632
Size: 876 215 888 1344 1384 268 1917
Fragments arranged by size:
1917 1384 1344 888 876 268 215
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6695
AflIII A'CryG_T
Cuts at: 1203 2709 5791 6768 1203
Size: 1506 3082 977 1327
Fragments arranged by size:
3082 1506 1327 977
AgeI A'CCGG_T
Cuts at: 2241 5723 2241
Size: 3482 3410
AhdI GACnn_n'nnGTC
Cuts at: 5621 5621
Size: 6892
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6892
AlwNI CAG_nnn'CTG
Cuts at: 3689 3989 4043 4635 6207 3689
Size: 300 54 592 1572 4374
Fragments arranged by size:
4374 1572 592 300 54
ApaLI G'TGCA_C
Cuts at: 4949 6105 4949
Size: 1156 5736
ApoI r'AATT_y
Cuts at: 1493 2302 3202 4201 4427 4579 6688 1493
Size: 809 900 999 226 152 2109 1697
Fragments arranged by size:
2109 1697 999 900 809 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5446 160
Size: 872 4414 1606
Fragments arranged by size:
4414 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6892
AvaI C'yCGr_G
Cuts at: 1870 1943 2484 2686 4217 1870
Size: 73 541 202 1531 4545
Fragments arranged by size:
4545 1531 541 202 73
BamHI G'GATC_C
Cuts at: 4222 4589 4222
Size: 367 6525
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3650 4213 5668 618
Size: 325 217 627 1863 563 1455 1842
Fragments arranged by size:
1863 1842 1455 627 563 325 217
BanII G_rGCy'C
Cuts at: 1391 2102 2346 2412 2900 3044 3873 4211 1391
Size: 711 244 66 488 144 829 338 4072
Fragments arranged by size:
4072 829 711 488 338 244 144 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2487 4182 928
Size: 971 588 1695 3638
Fragments arranged by size:
3638 1695 971 588
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5083 5117 1903
Size: 34 3146 34 3678
Fragments arranged by size:
3678 3146 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4784 5994 3686
Size: 1098 1210 4584
Fragments arranged by size:
4584 1210 1098
BclI T'GATC_A
Cuts at: 1548 3799 1548
Size: 2251 4641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2267 5503 136
Size: 107 122 71 631 605 595 3236 1525
Fragments arranged by size:
3236 1525 631 605 595 122 107 71
BglII A'GATC_T
Cuts at: 6887 6887
Size: 6892
BlpI GC'TnA_GC
Cuts at: 2153 4309 2153
Size: 2156 4736
Bme1580I G_kGCm'C
Cuts at: 1790 4953 6109 1790
Size: 3163 1156 2573
Fragments arranged by size:
3163 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6892
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3850 4623 5581
Size: 416 53 83 399 24 2433 773 958
Cuts at: 5581 442
Size: 1753
Fragments arranged by size:
2433 1753 958 773 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6892
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3060 3060
Size: 6892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2444 3138 3654 4065 5552 1239
Size: 812 393 694 516 411 1487 2579
Fragments arranged by size:
2579 1487 812 694 516 411 393
Bpu10I CC'TnA_GC
Cuts at: 2983 2983
Size: 6892
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 3686 3950 5017 5882 6180 2330
Size: 1356 264 1067 865 298 3042
Fragments arranged by size:
3042 1356 1067 865 298 264
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3411 5555 882
Size: 617 1912 2144 2219
Fragments arranged by size:
2219 2144 1912 617
BsaAI yAC'GTr
Cuts at: 493 1204 3664 4683 493
Size: 711 2460 1019 2702
Fragments arranged by size:
2702 2460 1019 711
BsaBI GATnn'nnATC
Cuts at: 3900 6886 3900
Size: 2986 3906
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3465 4699 5081 5716
Size: 53 83 186 936 1932 1234 382 635
Cuts at: 5716 275
Size: 1451
Fragments arranged by size:
1932 1451 1234 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2241 2782 5325 5723 5997 6144 6555 2241
Size: 541 2543 398 274 147 411 2578
Fragments arranged by size:
2578 2543 541 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3111 3141 4038 4068 1178
Size: 30 165 30 1708 30 897 30 4002
Fragments arranged by size:
4002 1708 897 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3049 3052 3377 1186
Size: 1863 3 325 4701
Fragments arranged by size:
4701 1863 325 3
BseYI C'CCAG_C
Cuts at: 3595 6095 6591 3595
Size: 2500 496 3896
Fragments arranged by size:
3896 2500 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6319
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4342 5103 6131 1540
Size: 275 186 2341 761 1028 2301
Fragments arranged by size:
2341 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2037 2888 3044 3297 3570 3873 4953 5038 6109
Size: 851 156 253 273 303 1080 85 1071
Cuts at: 6109 2037
Size: 2820
Fragments arranged by size:
2820 1080 1071 851 303 273 253 156
85
BsmI GAATG_Cn'
Cuts at: 4403 4496 4403
Size: 93 6799
BsmBI CGTCTCn'nnnn_
Cuts at: 3456 3456
Size: 6892
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2102 2346 2412 2888 2900 3044
Size: 399 247 65 244 66 476 12 144
Cuts at: 3044 3297 3570 3873 4211 4953 5038 6109 1391
Size: 253 273 303 338 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 476 399 338 303 273
253 247 244 144 85 66 65 12
BspEI T'CCGG_A
Cuts at: 6555 6555
Size: 6892
BspHI T'CATG_A
Cuts at: 4781 6511 4781
Size: 1730 5162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2638 3516 3561 3713 3808 4152 4247 844
Size: 1794 878 45 152 95 344 95 3489
Fragments arranged by size:
3489 1794 878 344 152 95 95 45
BsrBI CCG'CTC
Cuts at: 1394 1823 2047 4144 4779 1394
Size: 429 224 2097 635 3507
Fragments arranged by size:
3507 2097 635 429 224
BsrDI GCAATG_nn'
Cuts at: 65 1248 3678 5387 5561 65
Size: 1183 2430 1709 174 1396
Fragments arranged by size:
2430 1709 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2241 5536 5723 1700
Size: 110 431 3295 187 2869
Fragments arranged by size:
3295 2869 431 187 110
BsrGI T'GTAC_A
Cuts at: 96 2706 3480 3636 96
Size: 2610 774 156 3352
Fragments arranged by size:
3352 2610 774 156
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6892
BssSI C'ACGA_G
Cuts at: 4952 5964 4952
Size: 1012 5880
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6892
BstBI TT'CG_AA
Cuts at: 5696 5696
Size: 6892
BstEII G'GTnAC_C
Cuts at: 1184 2566 1184
Size: 1382 5510
BstXI CCAn_nnnn'nTGG
Cuts at: 2680 2680
Size: 6892
BstYI r'GATC_y
Cuts at: 1972 4222 4589 4974 4991 6443 6529 6541 6887
Size: 2250 367 385 17 1452 86 12 346
Cuts at: 6887 1972
Size: 1977
Fragments arranged by size:
2250 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2594 3766 4069 513
Size: 552 1529 1172 303 3336
Fragments arranged by size:
3336 1529 1172 552 303
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6153
Fragments arranged by size:
6153 683 56
BtsI GCAGTG_nn'
Cuts at: 2116 2496 2505 4408 5202 5222 2116
Size: 380 9 1903 794 20 3786
Fragments arranged by size:
3786 1903 794 380 20 9
ClaI AT'CG_AT
Cuts at: 1554 2203 1554
Size: 649 6243
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3645 3680 4128 4163 529
Size: 35 3081 35 448 35 3258
Fragments arranged by size:
3258 3081 448 35 35 35
DraI TTT'AAA
Cuts at: 4196 4196
Size: 6892
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5899 809
Size: 507 4583 1802
Fragments arranged by size:
4583 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2502 3612 4339
Size: 54 1425 50 275 18 672 1110 727
Cuts at: 4339 5228 6857 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1110 889 727 672 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4339 1537
Size: 275 2527 4090
Fragments arranged by size:
4090 2527 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3322 4822 1353
Size: 1969 1500 3423
Fragments arranged by size:
3423 1969 1500
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5479 5853 5999 387
Size: 1064 138 3890 374 146 1280
Fragments arranged by size:
3890 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3042 3871 3042
Size: 829 6063
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2444 2508 2723 3138 3611 3654
Size: 393 419 393 64 215 415 473 43
Cuts at: 3654 4065 4955 5552 6339 6607 1239
Size: 411 890 597 787 268 1524
Fragments arranged by size:
1524 890 787 597 473 419 415 411
393 393 268 215 64 43
EcoO109I rG'GnC_Cy
Cuts at: 2260 2650 4019 2260
Size: 390 1369 5133
Fragments arranged by size:
5133 1369 390
EcoRI G'AATT_C
Cuts at: 4201 4579 4201
Size: 378 6514
EcoRV GAT'ATC
Cuts at: 2662 3538 2662
Size: 876 6016
FspI TGC'GCA
Cuts at: 5398 5398
Size: 6892
HaeII r_GCGC'y
Cuts at: 1678 2115 3498 6039 1678
Size: 437 1383 2541 2531
Fragments arranged by size:
2541 2531 1383 437
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2269 2301 3162 3194 3684
Size: 32 135 32 697 32 861 32 490
Cuts at: 3684 3716 4038 4070 4128 4160 5533 5565 5607
Size: 32 322 32 58 32 1373 32 42
Cuts at: 5607 5639 1373
Size: 32 2626
Fragments arranged by size:
2626 1373 861 697 490 322 135 58
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4236 4482 669
Size: 988 281 2298 246 3079
Fragments arranged by size:
3079 2298 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6892
HpaI GTT'AAC
Cuts at: 4482 4482
Size: 6892
KpnI G_GTAC'C
Cuts at: 4217 4217
Size: 6892
MfeI C'AATT_G
Cuts at: 4491 4491
Size: 6892
MluI A'CGCG_T
Cuts at: 6768 6768
Size: 6892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2271 2694 3111 3160 4194 6006 6190 1305
Size: 966 423 417 49 1034 1812 184 2007
Fragments arranged by size:
2007 1812 1034 966 423 417 184 49
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2504 3614 10
Size: 54 1425 343 672 1110 3288
Fragments arranged by size:
3288 1425 1110 672 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3573 3878 4850 5209 5368 518
Size: 648 2407 305 972 359 159 2042
Fragments arranged by size:
2407 2042 972 648 359 305 159
MspA1I CmG'CkG
Cuts at: 1606 3979 4043 4308 4985 6133 6378 1606
Size: 2373 64 265 677 1148 245 2120
Fragments arranged by size:
2373 2120 1148 677 265 245 64
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6340
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6892
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6892
NotI GC'GGCC_GC
Cuts at: 4339 4339
Size: 6892
NspI r_CATG'y
Cuts at: 1132 1229 2778 4250 5795 1132
Size: 97 1549 1472 1545 2229
Fragments arranged by size:
2229 1549 1545 1472 97
PasI CC'CwG_GG
Cuts at: 1348 2311 3939 1348
Size: 963 1628 4301
Fragments arranged by size:
4301 1628 963
PciI A'CATG_T
Cuts at: 5791 5791
Size: 6892
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2374 3767 1108
Size: 1266 1393 4233
Fragments arranged by size:
4233 1393 1266
PmeI GTTT'AAAC
Cuts at: 4196 4196
Size: 6892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4961 4993 6474 6506 4961
Size: 32 1481 32 5347
Fragments arranged by size:
5347 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2650 2650
Size: 6892
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6892
PsiI TTA'TAA
Cuts at: 4462 4462
Size: 6892
PstI C_TGCA'G
Cuts at: 830 2123 2148 3092 4244 830
Size: 1293 25 944 1152 3478
Fragments arranged by size:
3478 1293 1152 944 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6892
PvuII CAG'CTG
Cuts at: 3979 4043 3979
Size: 64 6828
SacI G_AGCT'C
Cuts at: 3044 3873 3044
Size: 829 6063
SalI G'TCGA_C
Cuts at: 1936 4234 1936
Size: 2298 4594
SbfI CC_TGCA'GG
Cuts at: 4244 4244
Size: 6892
ScaI AGT'ACT
Cuts at: 3927 5140 6852 3927
Size: 1213 1712 3967
Fragments arranged by size:
3967 1712 1213
SfcI C'TryA_G
Cuts at: 826 1045 2119 2144 3088 4240 5375 6056 6247
Size: 219 1074 25 944 1152 1135 681 191
Cuts at: 6247 826
Size: 1471
Fragments arranged by size:
1471 1152 1135 1074 944 681 219 191
25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6892
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6892
SmaI CCC'GGG
Cuts at: 1872 4219 1872
Size: 2347 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2345 3701 3929 4996 5897 6159
Size: 197 926 402 1356 228 1067 901 262
Cuts at: 6159 820
Size: 1553
Fragments arranged by size:
1553 1356 1067 926 901 402 262 228
197
SnaBI TAC'GTA
Cuts at: 493 4683 493
Size: 4190 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6892
SphI G_CATG'C
Cuts at: 1132 4250 1132
Size: 3118 3774
SspI AAT'ATT
Cuts at: 5 52 4816 5
Size: 47 4764 2081
Fragments arranged by size:
4764 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6892
StyI C'CwwG_G
Cuts at: 513 1065 4331 513
Size: 552 3266 3074
Fragments arranged by size:
3266 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3022 3587 4919 4936 5089 5274 5719
Size: 1255 1442 565 1332 17 153 185 445
Cuts at: 5719 325
Size: 1498
Fragments arranged by size:
1498 1442 1332 1255 565 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2427 2706 3480
Size: 274 80 33 51 1443 450 279 774
Cuts at: 3480 3636 3925 5138 6850 96
Size: 156 289 1213 1712 138
Fragments arranged by size:
1712 1443 1213 774 450 289 279 274
156 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3111 3143 6852 6884 3111
Size: 32 3709 32 3119
Fragments arranged by size:
3709 3119 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6892
XbaI T'CTAG_A
Cuts at: 4228 4228
Size: 6892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3886 4125 3886
Size: 239 6653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6892
XmaI C'CCGG_G
Cuts at: 1870 4217 1870
Size: 2347 4545
XmnI GAAnn'nnTTC
Cuts at: 2521 4007 5021 2521
Size: 1486 1014 4392
Fragments arranged by size:
4392 1486 1014
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4700 276
Size: 53 83 186 936 3166 2468
Fragments arranged by size:
3166 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI