Restriction map for
pFN21AA0363
(Circular) (Six-base) MAPSORT of: pFN21AA0363.seq Check: 333 from: 1 to: 9112
pFN21AA0363 9112 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6922 278
Size: 53 83 186 936 5386 2468
Fragments arranged by size:
5386 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6455 1937
Size: 4518 4594
Acc65I G'GTAC_C
Cuts at: 5423 6433 5423
Size: 1010 8102
AclI AA'CG_TT
Cuts at: 1563 7239 7612 1563
Size: 5676 373 3063
Fragments arranged by size:
5676 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2472 3636 3756 3876 3996 4116 4236 4356
Size: 840 1164 120 120 120 120 120 120
Cuts at: 4356 4476 4997 5175 7175 8559 8827 1632
Size: 120 521 178 2000 1384 268 1917
Fragments arranged by size:
2000 1917 1384 1164 840 521 268 178
120 120 120 120 120 120 120
AfeI AGC'GCT
Cuts at: 2139 2139
Size: 9112
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8915
AflIII A'CryG_T
Cuts at: 1203 8011 8988 1203
Size: 6808 977 1327
Fragments arranged by size:
6808 1327 977
AgeI A'CCGG_T
Cuts at: 7943 7943
Size: 9112
AhdI GACnn_n'nnGTC
Cuts at: 2794 7841 2794
Size: 5047 4065
AleI CACnn'nnGTG
Cuts at: 1166 3599 3719 3839 3959 4079 4199 4319 4439
Size: 2433 120 120 120 120 120 120 120
Cuts at: 4439 4905 5384 6107 1166
Size: 466 479 723 4171
Fragments arranged by size:
4171 2433 723 479 466 120 120 120
120 120 120 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5354 1184
Size: 4170 4942
AlwNI CAG_nnn'CTG
Cuts at: 2038 2234 2330 2818 4604 4661 4751 5223 5596
Size: 196 96 488 1786 57 90 472 373
Cuts at: 5596 6855 8427 2038
Size: 1259 1572 2723
Fragments arranged by size:
2723 1786 1572 1259 488 472 373 196
96 90 57
ApaI G_GGCC'C
Cuts at: 2304 5405 5727 5812 2304
Size: 3101 322 85 5604
Fragments arranged by size:
5604 3101 322 85
ApaLI G'TGCA_C
Cuts at: 6152 7169 8325 6152
Size: 1017 1156 6939
Fragments arranged by size:
6939 1156 1017
ApoI r'AATT_y
Cuts at: 1493 6421 6647 6799 8908 1493
Size: 4928 226 152 2109 1697
Fragments arranged by size:
4928 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7666 160
Size: 872 6634 1606
Fragments arranged by size:
6634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9112
AvaI C'yCGr_G
Cuts at: 1870 1943 2183 2374 5762 5805 5818 6228 6437
Size: 73 240 191 3388 43 13 410 209
Cuts at: 6437 1870
Size: 4545
Fragments arranged by size:
4545 3388 410 240 209 191 73 43
13
BamHI G'GATC_C
Cuts at: 2746 6442 6809 2746
Size: 3696 367 5049
Fragments arranged by size:
5049 3696 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5330 5423 6181 6433 7888
Size: 325 217 627 3543 93 758 252 1455
Cuts at: 7888 618
Size: 1842
Fragments arranged by size:
3543 1842 1455 758 627 325 252 217
93
BanII G_rGCy'C
Cuts at: 1391 2076 2304 2381 2544 2952 5090 5136 5405
Size: 685 228 77 163 408 2138 46 269
Cuts at: 5405 5460 5517 5727 5735 5754 5812 5819 6092
Size: 55 57 210 8 19 58 7 273
Cuts at: 6092 6195 6361 6431 1391
Size: 103 166 70 4072
Fragments arranged by size:
4072 2138 685 408 273 269 228 210
166 163 103 77 70 58 57 55
46 19 8 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2019 2996 5181 5499 5511 5802 6073
Size: 971 120 977 2185 318 12 291 271
Cuts at: 6073 928
Size: 3967
Fragments arranged by size:
3967 2185 977 971 318 291 271 120
12
BbvCI CC'TCA_GC
Cuts at: 2432 3300 5146 5642 6196 2432
Size: 868 1846 496 554 5348
Fragments arranged by size:
5348 1846 868 554 496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2840 2861 2874 2895 7303
Size: 34 50 34 819 21 13 21 4408
Cuts at: 7303 7337 1903
Size: 34 3678
Fragments arranged by size:
4408 3678 819 50 34 34 34 21
21 13
BciVI GTATCCnnnnn_n'
Cuts at: 7004 8214 7004
Size: 1210 7902
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2598 3062 4616
Size: 107 122 71 631 605 926 464 1554
Cuts at: 4616 5115 5162 5739 5981 6356 7723 136
Size: 499 47 577 242 375 1367 1525
Fragments arranged by size:
1554 1525 1367 926 631 605 577 499
464 375 242 122 107 71 47
BglII A'GATC_T
Cuts at: 3327 3559 3799 3919 4039 4159 4279 4399 5688
Size: 232 240 120 120 120 120 120 1289
Cuts at: 5688 9107 3327
Size: 3419 3332
Fragments arranged by size:
3419 3332 1289 240 232 120 120 120
120 120
BlpI GC'TnA_GC
Cuts at: 2331 4620 4917 6529 2331
Size: 2289 297 1612 4914
Fragments arranged by size:
4914 2289 1612 297
Bme1580I G_kGCm'C
Cuts at: 1790 2163 2304 4591 5405 5727 5812 6156 6186
Size: 373 141 2287 814 322 85 344 30
Cuts at: 6186 7173 8329 1790
Size: 987 1156 2573
Fragments arranged by size:
2573 2287 1156 987 814 373 344 322
141 85 30
BmgBI CAC'GTC
Cuts at: 1318 2100 2796 5247 1318
Size: 782 696 2451 5183
Fragments arranged by size:
5183 2451 782 696
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5291 5313 5613
Size: 416 53 83 399 24 3874 22 300
Cuts at: 5613 6843 7801 442
Size: 1230 958 1753
Fragments arranged by size:
3874 1753 1230 958 416 399 300 83
53 24 22
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4843 5636 5815 4843
Size: 793 179 8140
Fragments arranged by size:
8140 793 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2957 3191 4967 5474 5810 6260 7772
Size: 851 867 234 1776 507 336 450 1512
Cuts at: 7772 1239
Size: 2579
Fragments arranged by size:
2579 1776 1512 867 851 507 450 336
234
Bpu10I CC'TnA_GC
Cuts at: 2141 2432 3033 3300 4818 5084 5146 5642 6196
Size: 291 601 267 1518 266 62 496 554
Cuts at: 6196 2141
Size: 5057
Fragments arranged by size:
5057 1518 601 554 496 291 267 266
62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5730 7237 8102 8400 5730
Size: 1507 865 298 6442
Fragments arranged by size:
6442 1507 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4934 5927 7775 882
Size: 617 3435 993 1848 2219
Fragments arranged by size:
3435 2219 1848 993 617
BsaAI yAC'GTr
Cuts at: 493 1204 6903 493
Size: 711 5699 2702
Fragments arranged by size:
5699 2702 711
BsaBI GATnn'nnATC
Cuts at: 6032 9106 6032
Size: 3074 6038
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2313 6919 7301 7936
Size: 53 83 186 936 780 4606 382 635
Cuts at: 7936 275
Size: 1451
Fragments arranged by size:
4606 1451 936 780 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7545 7943 8217 8364 8775 7545
Size: 398 274 147 411 7882
Fragments arranged by size:
7882 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2035 2065 2564 2594 2891
Size: 30 165 30 632 30 499 30 297
Cuts at: 2891 2921 1178
Size: 30 7369
Fragments arranged by size:
7369 632 499 297 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2000 2043 2108 2572 2747 2798 2878 3011
Size: 814 43 65 464 175 51 80 133
Cuts at: 3011 3014 3060 3399 4679 5525 5630 5703 5874
Size: 3 46 339 1280 846 105 73 171
Cuts at: 5874 5945 6275 6278 6281 1186
Size: 71 330 3 3 4017
Fragments arranged by size:
4017 1280 846 814 464 339 330 175
171 133 105 80 73 71 65 51
46 43 3 3 3
BseYI C'CCAG_C
Cuts at: 2406 2475 2524 2771 3242 3606 3726 3846 3966
Size: 69 49 247 471 364 120 120 120
Cuts at: 3966 4086 4206 4326 4446 4751 4835 5089 5262
Size: 120 120 120 120 305 84 254 173
Cuts at: 5262 5375 5432 5461 5734 5914 5995 6091 6144
Size: 113 57 29 273 180 81 96 53
Cuts at: 6144 8315 8811 2406
Size: 2171 496 2707
Fragments arranged by size:
2707 2171 496 471 364 305 273 254
247 180 173 120 120 120 120 120
120 120 113 96 84 81 69 57
53 49 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2504 5235 5915 1163
Size: 573 768 2731 680 4360
Fragments arranged by size:
4360 2731 768 680 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2394 6562 7323 8351 1540
Size: 275 186 393 4168 761 1028 2301
Fragments arranged by size:
4168 2301 1028 761 393 275 186
BsiHKAI G_wGCw'C
Cuts at: 6156 7173 7258 8329 6156
Size: 1017 85 1071 6939
Fragments arranged by size:
6939 1071 1017 85
BsmI GAATG_Cn'
Cuts at: 4678 5827 6623 6716 4678
Size: 1149 796 93 7074
Fragments arranged by size:
7074 1149 796 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2090 2090
Size: 9112
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2163 2304 2381 2544 2952 4591
Size: 399 286 87 141 77 163 408 1639
Cuts at: 4591 5090 5136 5405 5460 5517 5727 5735 5754
Size: 499 46 269 55 57 210 8 19
Cuts at: 5754 5812 5819 6092 6156 6186 6195 6361 6431
Size: 58 7 273 64 30 9 166 70
Cuts at: 6431 7173 7258 8329 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1639 1071 742 499 408 399 286
273 269 210 166 163 141 87 85
77 70 64 58 57 55 46 30
19 9 8 7
BspEI T'CCGG_A
Cuts at: 8775 8775
Size: 9112
BspHI T'CATG_A
Cuts at: 7001 8731 7001
Size: 1730 7382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3181 6467 844
Size: 2337 3286 3489
Fragments arranged by size:
3489 3286 2337
BsrBI CCG'CTC
Cuts at: 1394 1823 3006 6999 1394
Size: 429 1183 3993 3507
Fragments arranged by size:
3993 3507 1183 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3300 5991 7607 7781 65
Size: 1183 2052 2691 1616 174 1396
Fragments arranged by size:
2691 2052 1616 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4822 7756 7943 1700
Size: 110 3012 2934 187 2869
Fragments arranged by size:
3012 2934 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2937 96
Size: 2841 6271
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9112
BssSI C'ACGA_G
Cuts at: 7172 8184 7172
Size: 1012 8100
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4586 5692 794
Size: 3792 1106 4214
Fragments arranged by size:
4214 3792 1106
BstBI TT'CG_AA
Cuts at: 7916 7916
Size: 9112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9112
BstXI CCAn_nnnn'nTGG
Cuts at: 2156 5677 2156
Size: 3521 5591
BstYI r'GATC_y
Cuts at: 1972 2746 3327 3559 3799 3919 4039 4159 4279
Size: 774 581 232 240 120 120 120 120
Cuts at: 4279 4399 5688 6442 6809 7194 7211 8663 8749
Size: 120 1289 754 367 385 17 1452 86
Cuts at: 8749 8761 9107 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1289 774 754 581 385 367
346 240 232 120 120 120 120 120
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2016 2470 4528 4642 4662 5061 2016
Size: 454 2058 114 20 399 6067
Fragments arranged by size:
6067 2058 454 399 114 20
BtgI C'CryG_G
Cuts at: 513 1065 2043 2305 2499 3057 3288 4614 5385
Size: 552 978 262 194 558 231 1326 771
Cuts at: 5385 5661 5839 6408 513
Size: 276 178 569 3217
Fragments arranged by size:
3217 1326 978 771 569 558 552 276
262 231 194 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5923 6049 487
Size: 683 56 4697 126 3550
Fragments arranged by size:
4697 3550 683 126 56
BtsI GCAGTG_nn'
Cuts at: 2700 3162 3348 5299 5903 5932 5968 6628 7422
Size: 462 186 1951 604 29 36 660 794
Cuts at: 7422 7442 2700
Size: 20 4370
Fragments arranged by size:
4370 1951 794 660 604 462 186 36
29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3564 3599 3684 3719 3804 3839 3924
Size: 35 3000 35 85 35 85 35 85
Cuts at: 3924 3959 4044 4079 4164 4199 4284 4319 4404
Size: 35 85 35 85 35 85 35 85
Cuts at: 4404 4439 529
Size: 35 5202
Fragments arranged by size:
5202 3000 85 85 85 85 85 85
85 35 35 35 35 35 35 35
35 35
DraI TTT'AAA
Cuts at: 6416 6416
Size: 9112
DraIII CAC_nnn'GTG
Cuts at: 3396 3396
Size: 9112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8119 809
Size: 507 6803 1802
Fragments arranged by size:
6803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2391 2625
Size: 54 1425 50 275 18 231 330 234
Cuts at: 2625 4551 4608 5154 6559 7448 9077 8
Size: 1926 57 546 1405 889 1629 43
Fragments arranged by size:
1926 1629 1425 1405 889 546 330 275
234 231 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2391 6559 1537
Size: 275 579 4168 4090
Fragments arranged by size:
4168 4090 579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2419 2724 3129 3612 3732 3852 3972 4092
Size: 1066 305 405 483 120 120 120 120
Cuts at: 4092 4212 4332 4452 6246 6252 6267 7042 1353
Size: 120 120 120 1794 6 15 775 3423
Fragments arranged by size:
3423 1794 1066 775 483 405 305 120
120 120 120 120 120 120 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2759 7699 8073 8219 387
Size: 1064 138 1170 4940 374 146 1280
Fragments arranged by size:
4940 1280 1170 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2472 2957 3191 3636 3756 3876
Size: 393 458 382 485 234 445 120 120
Cuts at: 3876 3996 4116 4236 4356 4476 4967 4997 5175
Size: 120 120 120 120 120 491 30 178
Cuts at: 5175 5474 5810 6260 7175 7772 8559 8827 1239
Size: 299 336 450 915 597 787 268 1524
Fragments arranged by size:
1524 915 787 597 491 485 458 450
445 393 382 336 299 268 234 178
120 120 120 120 120 120 120 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2015 5363 5477 2015
Size: 3348 114 5650
Fragments arranged by size:
5650 3348 114
EcoO109I rG'GnC_Cy
Cuts at: 2020 2107 2301 2479 2532 2764 3090 3139 3502
Size: 87 194 178 53 232 326 49 363
Cuts at: 3502 3622 3742 3862 3982 4102 4222 4342 4462
Size: 120 120 120 120 120 120 120 120
Cuts at: 4462 4520 4646 4804 5032 5394 5402 5482 5724
Size: 58 126 158 228 362 8 80 242
Cuts at: 5724 5809 5911 6136 6225 2020
Size: 85 102 225 89 4907
Fragments arranged by size:
4907 363 362 326 242 232 228 225
194 178 158 126 120 120 120 120
120 120 120 120 102 89 87 85
80 58 53 49 8
EcoRI G'AATT_C
Cuts at: 6421 6799 6421
Size: 378 8734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3158 6016 3158
Size: 2858 6254
FspI TGC'GCA
Cuts at: 7618 7618
Size: 9112
HaeII r_GCGC'y
Cuts at: 1678 2141 8259 1678
Size: 463 6118 2531
Fragments arranged by size:
6118 2531 463
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2453 2485 2531
Size: 32 135 32 437 32 412 32 46
Cuts at: 2531 2563 2564 2596 2780 2812 2861 2885 2891
Size: 32 1 32 184 32 49 24 6
Cuts at: 2891 2893 2917 2923 3431 3463 3523 3551 3555
Size: 2 24 6 508 32 60 28 4
Cuts at: 3555 3583 3643 3675 3763 3791 3795 3823 3883
Size: 28 60 32 88 28 4 28 60
Cuts at: 3883 3911 3915 3943 4003 4031 4035 4063 4123
Size: 28 4 28 60 28 4 28 60
Cuts at: 4123 4151 4155 4183 4243 4271 4275 4303 4363
Size: 28 4 28 60 28 4 28 60
Cuts at: 4363 4391 4395 4423 4483 4515 6125 6157 7753
Size: 28 4 28 60 32 1610 32 1596
Cuts at: 7753 7785 7827 7859 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1610 1596 508 437 412 184 135
88 60 60 60 60 60 60 60
60 49 46 42 32 32 32 32
32 32 32 32 32 32 32 32
32 28 28 28 28 28 28 28
28 28 28 28 28 28 28 24
24 6 6 4 4 4 4 4
4 4 2 1
HincII GTy'rAC
Cuts at: 669 1657 1938 6388 6456 6702 669
Size: 988 281 4450 68 246 3079
Fragments arranged by size:
4450 3079 988 281 246 68
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9112
HpaI GTT'AAC
Cuts at: 6702 6702
Size: 9112
KpnI G_GTAC'C
Cuts at: 5427 6437 5427
Size: 1010 8102
MfeI C'AATT_G
Cuts at: 6711 6711
Size: 9112
MluI A'CGCG_T
Cuts at: 8988 8988
Size: 9112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3389 4744 8226 8410 1305
Size: 2084 1355 3482 184 2007
Fragments arranged by size:
3482 2084 2007 1355 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2627 4553 4610 5156 10
Size: 54 1425 343 795 1926 57 546 3966
Fragments arranged by size:
3966 1926 1425 795 546 343 57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3293 3599 3719 3839 3959 4079 4199
Size: 648 2127 306 120 120 120 120 120
Cuts at: 4199 4319 4439 4905 5384 6107 7070 7429 7588
Size: 120 120 466 479 723 963 359 159
Cuts at: 7588 518
Size: 2042
Fragments arranged by size:
2127 2042 963 723 648 479 466 359
306 159 120 120 120 120 120 120
120
MspA1I CmG'CkG
Cuts at: 1606 2406 2943 3269 4742 5150 5567 5738 6167
Size: 800 537 326 1473 408 417 171 429
Cuts at: 6167 6528 7205 8353 8598 1606
Size: 361 677 1148 245 2120
Fragments arranged by size:
2120 1473 1148 800 677 537 429 417
408 361 326 245 171
NaeI GCC'GGC
Cuts at: 4824 4824
Size: 9112
NcoI C'CATG_G
Cuts at: 513 1065 2499 3288 4614 5661 6408 513
Size: 552 1434 789 1326 1047 747 3217
Fragments arranged by size:
3217 1434 1326 1047 789 747 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9112
NgoMIV G'CCGG_C
Cuts at: 4822 4822
Size: 9112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9112
NotI GC'GGCC_GC
Cuts at: 6559 6559
Size: 9112
NspI r_CATG'y
Cuts at: 1132 1229 4884 6470 8015 1132
Size: 97 3655 1586 1545 2229
Fragments arranged by size:
3655 2229 1586 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4592 5398 5812 1348
Size: 3244 806 414 4648
Fragments arranged by size:
4648 3244 806 414
PciI A'CATG_T
Cuts at: 8011 8011
Size: 9112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3242 1108
Size: 2134 6978
PfoI T'CCnGG_A
Cuts at: 2865 2973 4692 2865
Size: 108 1719 7285
Fragments arranged by size:
7285 1719 108
PmeI GTTT'AAAC
Cuts at: 6416 6416
Size: 9112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7181 7213 8694 8726 7181
Size: 32 1481 32 7567
Fragments arranged by size:
7567 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2532 2764 3502 5032 5482 6136 2532
Size: 232 738 1530 450 654 5508
Fragments arranged by size:
5508 1530 738 654 450 232
PshAI GACnn'nnGTC
Cuts at: 1613 5005 1613
Size: 3392 5720
PsiI TTA'TAA
Cuts at: 6682 6682
Size: 9112
PspOMI G'GGCC_C
Cuts at: 2300 5401 5723 5808 2300
Size: 3101 322 85 5604
Fragments arranged by size:
5604 3101 322 85
PstI C_TGCA'G
Cuts at: 830 2572 2814 2884 3030 3192 3355 3472 3592
Size: 1742 242 70 146 162 163 117 120
Cuts at: 3592 3712 3832 3952 4072 4192 4312 4432 4705
Size: 120 120 120 120 120 120 120 273
Cuts at: 4705 4747 5346 6464 830
Size: 42 599 1118 3478
Fragments arranged by size:
3478 1742 1118 599 273 242 163 162
146 120 120 120 120 120 120 120
120 117 70 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9112
PvuII CAG'CTG
Cuts at: 2406 4742 5150 5738 6167 2406
Size: 2336 408 588 429 5351
Fragments arranged by size:
5351 2336 588 429 408
SalI G'TCGA_C
Cuts at: 1936 6454 1936
Size: 4518 4594
SanDI GG'GwC_CC
Cuts at: 2532 2532
Size: 9112
SbfI CC_TGCA'GG
Cuts at: 6464 6464
Size: 9112
ScaI AGT'ACT
Cuts at: 7360 9072 7360
Size: 1712 7400
SexAI A'CCwGG_T
Cuts at: 4713 4713
Size: 9112
SfcI C'TryA_G
Cuts at: 826 1045 2568 2810 2880 3026 3188 3351 3462
Size: 219 1523 242 70 146 162 163 111
Cuts at: 3462 3468 3588 3708 3828 3948 4068 4188 4308
Size: 6 120 120 120 120 120 120 120
Cuts at: 4308 4428 4686 4701 4743 5342 6460 7595 8276
Size: 120 258 15 42 599 1118 1135 681
Cuts at: 8276 8467 826
Size: 191 1471
Fragments arranged by size:
1523 1471 1135 1118 681 599 258 242
219 191 163 162 146 120 120 120
120 120 120 120 120 111 70 42
15 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2598 5162 2598
Size: 2564 6548
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9112
SmaI CCC'GGG
Cuts at: 1872 6230 6439 1872
Size: 4358 209 4545
Fragments arranged by size:
4545 4358 209
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5745 7216 8117 8379 820
Size: 197 926 3802 1471 901 262 1553
Fragments arranged by size:
3802 1553 1471 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6903 493
Size: 6410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9112
SphI G_CATG'C
Cuts at: 1132 6470 1132
Size: 5338 3774
SspI AAT'ATT
Cuts at: 5 52 7036 5
Size: 47 6984 2081
Fragments arranged by size:
6984 2081 47
StuI AGG'CCT
Cuts at: 1517 3204 4640 4867 4974 5109 1517
Size: 1687 1436 227 107 135 5520
Fragments arranged by size:
5520 1687 1436 227 135 107
StyI C'CwwG_G
Cuts at: 513 1065 2499 3018 3288 4614 4707 4760 5326
Size: 552 1434 519 270 1326 93 53 566
Cuts at: 5326 5364 5649 5661 6201 6345 6408 6551 513
Size: 38 285 12 540 144 63 143 3074
Fragments arranged by size:
3074 1434 1326 566 552 540 519 285
270 144 143 93 63 53 38 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2134 2538 6235 7139 7156 7309 7494
Size: 1255 554 404 3697 904 17 153 185
Cuts at: 7494 7939 325
Size: 445 1498
Fragments arranged by size:
3697 1498 1255 904 554 445 404 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2937 5278 7358
Size: 274 80 33 51 1443 960 2341 2080
Cuts at: 7358 9070 96
Size: 1712 138
Fragments arranged by size:
2341 2080 1712 1443 960 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2035 2067 3382 3414 4516 4548 9072 9104 2035
Size: 32 1315 32 1102 32 4524 32 2043
Fragments arranged by size:
4524 2043 1315 1102 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2981 3046 4715 6383 1316
Size: 1665 65 1669 1668 4045
Fragments arranged by size:
4045 1669 1668 1665 65
XbaI T'CTAG_A
Cuts at: 6448 6448
Size: 9112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4795 5383 5593 5735 4795
Size: 588 210 142 8172
Fragments arranged by size:
8172 588 210 142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9112
XmaI C'CCGG_G
Cuts at: 1870 6228 6437 1870
Size: 4358 209 4545
Fragments arranged by size:
4545 4358 209
XmnI GAAnn'nnTTC
Cuts at: 3360 7241 3360
Size: 3881 5231
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6920 276
Size: 53 83 186 936 5386 2468
Fragments arranged by size:
5386 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SapI SfoI SrfI SwaI