Restriction map for
pFN21AA0361
(Circular) (Six-base) MAPSORT of: pFN21AA0361.seq Check: 1599 from: 1 to: 8719
pFN21AA0361 8719 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3403 3403
Size: 8719
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6529 278
Size: 53 83 186 936 4993 2468
Fragments arranged by size:
4993 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3372 6062 1937
Size: 1435 2690 4594
Fragments arranged by size:
4594 2690 1435
Acc65I G'GTAC_C
Cuts at: 6040 6040
Size: 8719
AclI AA'CG_TT
Cuts at: 1563 3751 6846 7219 1563
Size: 2188 3095 373 3063
Fragments arranged by size:
3095 3063 2188 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4560 4663 4919 5338 5446 6782 8166 8434
Size: 2928 103 256 419 108 1336 1384 268
Cuts at: 8434 1632
Size: 1917
Fragments arranged by size:
2928 1917 1384 1336 419 268 256 108
103
AfeI AGC'GCT
Cuts at: 2621 4037 2621
Size: 1416 7303
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8522
AflIII A'CryG_T
Cuts at: 1203 2911 7618 8595 1203
Size: 1708 4707 977 1327
Fragments arranged by size:
4707 1708 1327 977
AgeI A'CCGG_T
Cuts at: 7550 7550
Size: 8719
AhdI GACnn_n'nnGTC
Cuts at: 2915 7448 2915
Size: 4533 4186
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8719
AlwNI CAG_nnn'CTG
Cuts at: 2060 2740 3961 3997 4514 4696 4897 5399 6462
Size: 680 1221 36 517 182 201 502 1063
Cuts at: 6462 8034 2060
Size: 1572 2745
Fragments arranged by size:
2745 1572 1221 1063 680 517 502 201
182 36
ApaI G_GGCC'C
Cuts at: 2280 3028 4104 4962 5127 5552 2280
Size: 748 1076 858 165 425 5447
Fragments arranged by size:
5447 1076 858 748 425 165
ApaLI G'TGCA_C
Cuts at: 2744 3010 3986 6776 7932 2744
Size: 266 976 2790 1156 3531
Fragments arranged by size:
3531 2790 1156 976 266
ApoI r'AATT_y
Cuts at: 1493 2807 3124 6028 6254 6406 8515 1493
Size: 1314 317 2904 226 152 2109 1697
Fragments arranged by size:
2904 2109 1697 1314 317 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7273 160
Size: 872 6241 1606
Fragments arranged by size:
6241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8719
AvaI C'yCGr_G
Cuts at: 1870 1943 2226 2644 4434 4592 5193 6044 1870
Size: 73 283 418 1790 158 601 851 4545
Fragments arranged by size:
4545 1790 851 601 418 283 158 73
BamHI G'GATC_C
Cuts at: 6049 6416 6049
Size: 367 8352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3773 5675 6040 7495 618
Size: 325 217 627 1986 1902 365 1455 1842
Fragments arranged by size:
1986 1902 1842 1455 627 365 325 217
BanII G_rGCy'C
Cuts at: 1391 2280 3028 3891 4104 4158 4462 4577 4962
Size: 889 748 863 213 54 304 115 385
Cuts at: 4962 5032 5127 5378 5552 6038 1391
Size: 70 95 251 174 486 4072
Fragments arranged by size:
4072 889 863 748 486 385 304 251
213 174 115 95 70 54
BbeI G_GCGC'C
Cuts at: 5679 5679
Size: 8719
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2911 928
Size: 971 1012 6736
Fragments arranged by size:
6736 1012 971
BbvCI CC'TCA_GC
Cuts at: 2148 2166 2409 3704 4421 4845 5432 2148
Size: 18 243 1295 717 424 587 5435
Fragments arranged by size:
5435 1295 717 587 424 243 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6910 6944 1903
Size: 34 4973 34 3678
Fragments arranged by size:
4973 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3126 6611 7821 3126
Size: 3485 1210 4024
Fragments arranged by size:
4024 3485 1210
BclI T'GATC_A
Cuts at: 1548 3498 3550 1548
Size: 1950 52 6717
Fragments arranged by size:
6717 1950 52
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3976 4229 4361
Size: 107 122 71 631 605 2304 253 132
Cuts at: 4361 4947 5569 7330 136
Size: 586 622 1761 1525
Fragments arranged by size:
2304 1761 1525 631 622 605 586 253
132 122 107 71
BglII A'GATC_T
Cuts at: 8714 8714
Size: 8719
BlpI GC'TnA_GC
Cuts at: 3980 5427 6136 3980
Size: 1447 709 6563
Fragments arranged by size:
6563 1447 709
Bme1580I G_kGCm'C
Cuts at: 1790 2280 2748 3014 3028 3745 3776 3990 4104
Size: 490 468 266 14 717 31 214 114
Cuts at: 4104 4601 4962 5127 5169 5552 6780 7936 1790
Size: 497 361 165 42 383 1228 1156 2573
Fragments arranged by size:
2573 1228 1156 717 497 490 468 383
361 266 214 165 114 42 31 14
BmgBI CAC'GTC
Cuts at: 1318 5281 1318
Size: 3963 4756
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2006 2154 2346
Size: 416 53 83 399 24 589 148 192
Cuts at: 2346 2543 4606 5788 6450 7408 442
Size: 197 2063 1182 662 958 1753
Fragments arranged by size:
2063 1753 1182 958 662 589 416 399
197 192 148 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2576 3161 3218 3624 3804 3905 4019
Size: 1049 288 585 57 406 180 101 114
Cuts at: 4019 4626 4784 4886 4952 5024 5957 7379 1239
Size: 607 158 102 66 72 933 1422 2579
Fragments arranged by size:
2579 1422 1049 933 607 585 406 288
180 158 114 102 101 72 66 57
Bpu10I CC'TnA_GC
Cuts at: 2148 2166 2409 3704 4421 4720 4845 5432 5898
Size: 18 243 1295 717 299 125 587 466
Cuts at: 5898 2148
Size: 4969
Fragments arranged by size:
4969 1295 717 587 466 299 243 125
18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4514 6844 7709 8007 4514
Size: 2330 865 298 5226
Fragments arranged by size:
5226 2330 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 3459 3780 4442 5223 7382 882
Size: 617 858 1102 321 662 781 2159 2219
Fragments arranged by size:
2219 2159 1102 858 781 662 617 321
BsaAI yAC'GTr
Cuts at: 493 1204 2725 3031 5716 6510 493
Size: 711 1521 306 2685 794 2702
Fragments arranged by size:
2702 2685 1521 794 711 306
BsaBI GATnn'nnATC
Cuts at: 8713 8713
Size: 8719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2109 5676 6526 6908
Size: 53 83 186 936 576 3567 850 382
Cuts at: 6908 7543 275
Size: 635 1451
Fragments arranged by size:
3567 1451 936 850 635 576 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2445 3789 5442 5731 7152 7550 7824 7971 8382
Size: 1344 1653 289 1421 398 274 147 411
Cuts at: 8382 2445
Size: 2782
Fragments arranged by size:
2782 1653 1421 1344 411 398 289 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3354 3384 4062 4092 4734
Size: 30 165 30 1951 30 678 30 642
Cuts at: 4734 4764 1178
Size: 30 5133
Fragments arranged by size:
5133 1951 678 642 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 3477 4104 4166 4703 4862 5021 5054
Size: 997 1294 627 62 537 159 159 33
Cuts at: 5054 5108 5228 1186
Size: 54 120 4677
Fragments arranged by size:
4677 1294 997 627 537 159 159 120
62 54 33
BseYI C'CCAG_C
Cuts at: 2341 3598 3901 4083 4623 4936 5126 5186 5261
Size: 1257 303 182 540 313 190 60 75
Cuts at: 5261 5420 5508 5559 5619 7922 8418 2341
Size: 159 88 51 60 2303 496 2642
Fragments arranged by size:
2642 2303 1257 540 496 313 303 190
182 159 88 75 60 60 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2423 3431 3437 3476 4892 5759 1163
Size: 573 687 1008 6 39 1416 867 4123
Fragments arranged by size:
4123 1416 1008 867 687 573 39 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2446 3814 6169 6930 7958 1540
Size: 275 186 445 1368 2355 761 1028 2301
Fragments arranged by size:
2355 2301 1368 1028 761 445 275 186
BsiHKAI G_wGCw'C
Cuts at: 2651 2691 2748 2774 3014 3891 3990 4158 6780
Size: 40 57 26 240 877 99 168 2622
Cuts at: 6780 6865 7936 2651
Size: 85 1071 3434
Fragments arranged by size:
3434 2622 1071 877 240 168 99 85
57 40 26
BsmI GAATG_Cn'
Cuts at: 3658 6230 6323 3658
Size: 2572 93 6054
Fragments arranged by size:
6054 2572 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5036 5036
Size: 8719
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2280 2651 2691 2748 2774 3014 3028
Size: 399 490 371 40 57 26 240 14
Cuts at: 3028 3745 3776 3891 3990 4104 4158 4462 4577
Size: 717 31 115 99 114 54 304 115
Cuts at: 4577 4601 4962 5032 5127 5169 5378 5552 6038
Size: 24 361 70 95 42 209 174 486
Cuts at: 6038 6780 6865 7936 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 717 490 486 399 371
361 304 240 209 174 115 115 114
99 95 85 70 57 54 42 40
31 26 24 14
BspEI T'CCGG_A
Cuts at: 5442 5731 8382 5442
Size: 289 2651 5779
Fragments arranged by size:
5779 2651 289
BspHI T'CATG_A
Cuts at: 2766 3547 6608 8338 2766
Size: 781 3061 1730 3147
Fragments arranged by size:
3147 3061 1730 781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2268 3403 6074 844
Size: 1424 1135 2671 3489
Fragments arranged by size:
3489 2671 1424 1135
BsrBI CCG'CTC
Cuts at: 1394 1823 3222 4062 5116 5902 6606 1394
Size: 429 1399 840 1054 786 704 3507
Fragments arranged by size:
3507 1399 1054 840 786 704 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2421 3574 3580 4924 7214 7388 65
Size: 1183 1173 1153 6 1344 2290 174 1396
Fragments arranged by size:
2290 1396 1344 1183 1173 1153 174 6
BsrFI r'CCGG_y
Cuts at: 1700 1810 2379 2544 3047 4587 5061 7363 7550
Size: 110 569 165 503 1540 474 2302 187
Cuts at: 7550 1700
Size: 2869
Fragments arranged by size:
2869 2302 1540 569 503 474 187 165
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8719
BssSI C'ACGA_G
Cuts at: 4859 6779 7791 4859
Size: 1920 1012 5787
Fragments arranged by size:
5787 1920 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8719
BstBI TT'CG_AA
Cuts at: 7523 7523
Size: 8719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8719
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 2428 2576 2729 3119 3439 4054 4177 5261
Size: 87 148 153 390 320 615 123 1084
Cuts at: 5261 5764 5778 2341
Size: 503 14 5282
Fragments arranged by size:
5282 1084 615 503 390 320 153 148
123 87 14
BstYI r'GATC_y
Cuts at: 1972 3100 6049 6416 6801 6818 8270 8356 8368
Size: 1128 2949 367 385 17 1452 86 12
Cuts at: 8368 8714 1972
Size: 346 1977
Fragments arranged by size:
2949 1977 1452 1128 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2862 5108 2862
Size: 2246 6473
BtgI C'CryG_G
Cuts at: 513 1065 3019 3300 4023 4065 4233 4968 5329
Size: 552 1954 281 723 42 168 735 361
Cuts at: 5329 513
Size: 3903
Fragments arranged by size:
3903 1954 735 723 552 361 281 168
42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7980
Fragments arranged by size:
7980 683 56
BtsI GCAGTG_nn'
Cuts at: 2830 3042 4097 4380 4404 5771 5927 6235 7029
Size: 212 1055 283 24 1367 156 308 794
Cuts at: 7029 7049 2830
Size: 20 4500
Fragments arranged by size:
4500 1367 1055 794 308 283 212 156
24 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3858 3893 529
Size: 35 3294 35 5355
Fragments arranged by size:
5355 3294 35 35
DraI TTT'AAA
Cuts at: 6023 6023
Size: 8719
DraIII CAC_nnn'GTG
Cuts at: 3034 3034
Size: 8719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4214 7726 809
Size: 507 2898 3512 1802
Fragments arranged by size:
3512 2898 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2041 2876 3247
Size: 54 1425 50 275 18 211 835 371
Cuts at: 3247 3838 4026 4230 4236 5241 5450 5570 5869
Size: 591 188 204 6 1005 209 120 299
Cuts at: 5869 6166 7055 8684 8
Size: 297 889 1629 43
Fragments arranged by size:
1629 1425 1005 889 835 591 371 299
297 275 211 209 204 188 120 54
50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 6166 1537
Size: 275 4354 4090
Fragments arranged by size:
4354 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3702 6649 1353
Size: 2349 2947 3423
Fragments arranged by size:
3423 2947 2349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4364 7306 7680 7826 387
Size: 1064 138 2775 2942 374 146 1280
Fragments arranged by size:
2942 2775 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3889 4156 3889
Size: 267 8452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2576 3161 3218 3624 3804 3905
Size: 393 656 288 585 57 406 180 101
Cuts at: 3905 4019 4560 4626 4663 4784 4886 4919 4952
Size: 114 541 66 37 121 102 33 33
Cuts at: 4952 5024 5338 5446 5957 6782 7379 8166 8434
Size: 72 314 108 511 825 597 787 268
Cuts at: 8434 1239
Size: 1524
Fragments arranged by size:
1524 825 787 656 597 585 541 511
406 393 314 288 268 180 121 114
108 102 101 72 66 57 37 33
33
EcoNI CCTnn'n_nnAGG
Cuts at: 4889 5036 4889
Size: 147 8572
EcoO109I rG'GnC_Cy
Cuts at: 2152 2345 2599 2866 3024 3328 3365 3517 3640
Size: 193 254 267 158 304 37 152 123
Cuts at: 3640 3708 4100 4101 4959 5104 5123 5293 5549
Size: 68 392 1 858 145 19 170 256
Cuts at: 5549 5639 5832 2152
Size: 90 193 5039
Fragments arranged by size:
5039 858 392 304 267 256 254 193
193 170 158 152 145 123 90 68
37 19 1
EcoRI G'AATT_C
Cuts at: 6028 6406 6028
Size: 378 8341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3943 3943
Size: 8719
FspI TGC'GCA
Cuts at: 5700 7225 5700
Size: 1525 7194
FspAI rTGC'GCAy
Cuts at: 5700 5700
Size: 8719
HaeII r_GCGC'y
Cuts at: 1678 2377 2623 3561 4039 5440 5608 5679 7866
Size: 699 246 938 478 1401 168 71 2187
Cuts at: 7866 1678
Size: 2531
Fragments arranged by size:
2531 2187 1401 938 699 478 246 168
71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2901 2933 3354 3386 4537
Size: 32 135 32 1329 32 421 32 1151
Cuts at: 4537 4569 4734 4766 4814 4846 5187 5219 5285
Size: 32 165 32 48 32 341 32 66
Cuts at: 5285 5317 5395 5427 7360 7392 7434 7466 1373
Size: 32 78 32 1933 32 42 32 2626
Fragments arranged by size:
2626 1933 1329 1151 421 341 165 135
78 66 48 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5489 6063 6309 669
Size: 988 281 3551 574 246 3079
Fragments arranged by size:
3551 3079 988 574 281 246
HindIII A'AGCT_T
Cuts at: 748 3106 748
Size: 2358 6361
HpaI GTT'AAC
Cuts at: 6309 6309
Size: 8719
KasI G'GCGC_C
Cuts at: 5675 5675
Size: 8719
KpnI G_GTAC'C
Cuts at: 6044 6044
Size: 8719
MfeI C'AATT_G
Cuts at: 5310 6318 5310
Size: 1008 7711
MluI A'CGCG_T
Cuts at: 8595 8595
Size: 8719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3340 3367 7833 8017 1305
Size: 2035 27 4466 184 2007
Fragments arranged by size:
4466 2035 2007 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2043 3840 4028 10
Size: 54 1425 343 211 1797 188 4701
Fragments arranged by size:
4701 1797 1425 343 211 188 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2025 2727 3738 6677 7036 7195 518
Size: 648 859 702 1011 2939 359 159 2042
Fragments arranged by size:
2939 2042 1011 859 702 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2147 2408 2638 3021 3253 3602 5056 5396
Size: 541 261 230 383 232 349 1454 340
Cuts at: 5396 6135 6812 7960 8205 1606
Size: 739 677 1148 245 2120
Fragments arranged by size:
2120 1454 1148 739 677 541 383 349
340 261 245 232 230
NaeI GCC'GGC
Cuts at: 2546 3049 2546
Size: 503 8216
NarI GG'CG_CC
Cuts at: 5676 5676
Size: 8719
NcoI C'CATG_G
Cuts at: 513 1065 3300 4968 513
Size: 552 2235 1668 4264
Fragments arranged by size:
4264 2235 1668 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8719
NgoMIV G'CCGG_C
Cuts at: 2544 3047 2544
Size: 503 8216
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8719
NotI GC'GGCC_GC
Cuts at: 6166 6166
Size: 8719
NspI r_CATG'y
Cuts at: 1132 1229 2503 2915 4758 6077 7622 1132
Size: 97 1274 412 1843 1319 1545 2229
Fragments arranged by size:
2229 1843 1545 1319 1274 412 97
PasI CC'CwG_GG
Cuts at: 1348 3093 3974 4328 4387 4577 4815 5415 5837
Size: 1745 881 354 59 190 238 600 422
Cuts at: 5837 1348
Size: 4230
Fragments arranged by size:
4230 1745 881 600 422 354 238 190
59
PciI A'CATG_T
Cuts at: 2911 7618 2911
Size: 4707 4012
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3034 3997 4930 4969 5388 1108
Size: 1926 963 933 39 419 4439
Fragments arranged by size:
4439 1926 963 933 419 39
PfoI T'CCnGG_A
Cuts at: 2625 4281 2625
Size: 1656 7063
PmeI GTTT'AAAC
Cuts at: 6023 6023
Size: 8719
PmlI CAC'GTG
Cuts at: 2725 3031 2725
Size: 306 8413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6788 6820 8301 8333 6788
Size: 32 1481 32 7174
Fragments arranged by size:
7174 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2599 2866 3365 3640 3708 5293 5832 2599
Size: 267 499 275 68 1585 539 5486
Fragments arranged by size:
5486 1585 539 499 275 267 68
PshAI GACnn'nnGTC
Cuts at: 1613 3734 1613
Size: 2121 6598
PsiI TTA'TAA
Cuts at: 6289 6289
Size: 8719
PspOMI G'GGCC_C
Cuts at: 2276 3024 4100 4958 5123 5548 2276
Size: 748 1076 858 165 425 5447
Fragments arranged by size:
5447 1076 858 748 425 165
PstI C_TGCA'G
Cuts at: 830 2106 3542 3936 3957 5542 6071 830
Size: 1276 1436 394 21 1585 529 3478
Fragments arranged by size:
3478 1585 1436 1276 529 394 21
PvuI CG_AT'CG
Cuts at: 2001 3814 2001
Size: 1813 6906
PvuII CAG'CTG
Cuts at: 2147 2408 3602 5056 5396 2147
Size: 261 1194 1454 340 5470
Fragments arranged by size:
5470 1454 1194 340 261
SacI G_AGCT'C
Cuts at: 3891 4158 3891
Size: 267 8452
SacII CC_GC'GG
Cuts at: 3022 3022
Size: 8719
SalI G'TCGA_C
Cuts at: 1936 6061 1936
Size: 4125 4594
SanDI GG'GwC_CC
Cuts at: 3640 5832 3640
Size: 2192 6527
SapI GCTCTTCn'nnn_
Cuts at: 3702 3702
Size: 8719
SbfI CC_TGCA'GG
Cuts at: 6071 6071
Size: 8719
ScaI AGT'ACT
Cuts at: 6967 8679 6967
Size: 1712 7007
SexAI A'CCwGG_T
Cuts at: 4254 4254
Size: 8719
SfcI C'TryA_G
Cuts at: 826 1045 2102 2633 3373 3538 3932 3953 4793
Size: 219 1057 531 740 165 394 21 840
Cuts at: 4793 5066 5538 6067 7202 7883 8074 826
Size: 273 472 529 1135 681 191 1471
Fragments arranged by size:
1471 1135 1057 840 740 681 531 529
472 394 273 219 191 165 21
SfoI GGC'GCC
Cuts at: 5677 5677
Size: 8719
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8719
SmaI CCC'GGG
Cuts at: 1872 6046 1872
Size: 4174 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4529 6823 7724 7986 820
Size: 197 926 2586 2294 901 262 1553
Fragments arranged by size:
2586 2294 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5716 6510 493
Size: 5223 794 2702
Fragments arranged by size:
5223 2702 794
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8719
SphI G_CATG'C
Cuts at: 1132 2503 6077 1132
Size: 1371 3574 3774
Fragments arranged by size:
3774 3574 1371
SspI AAT'ATT
Cuts at: 5 52 6643 5
Size: 47 6591 2081
Fragments arranged by size:
6591 2081 47
StuI AGG'CCT
Cuts at: 1517 2660 4074 4746 4857 5577 5914 1517
Size: 1143 1414 672 111 720 337 4322
Fragments arranged by size:
4322 1414 1143 720 672 337 111
StyI C'CwwG_G
Cuts at: 513 1065 3225 3300 3522 4176 4968 5915 6158
Size: 552 2160 75 222 654 792 947 243
Cuts at: 6158 513
Size: 3074
Fragments arranged by size:
3074 2160 947 792 654 552 243 222
75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2525 2789 3886 4379 5500 6746 6763
Size: 1255 945 264 1097 493 1121 1246 17
Cuts at: 6763 6916 7101 7546 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1246 1121 1097 945 493 445
264 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6965 8677 96
Size: 274 80 33 51 1443 4988 1712 138
Fragments arranged by size:
4988 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4062 4094 4277 4309 8679 8711 4062
Size: 32 183 32 4370 32 4070
Fragments arranged by size:
4370 4070 183 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8719
XbaI T'CTAG_A
Cuts at: 6055 6055
Size: 8719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3035 5597 5632 3035
Size: 2562 35 6122
Fragments arranged by size:
6122 2562 35
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8719
XmaI C'CCGG_G
Cuts at: 1870 6044 1870
Size: 4174 4545
XmnI GAAnn'nnTTC
Cuts at: 3923 6848 3923
Size: 2925 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6527 276
Size: 53 83 186 936 4993 2468
Fragments arranged by size:
4993 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
EcoRV FseI NruI NsiI PacI PspXI PsrI RsrII
SfiI SrfI SwaI