Restriction map for
pFN21AA0356
(Circular) (Six-base) MAPSORT of: pFN21AA0356.seq Check: 4275 from: 1 to: 7873
pFN21AA0356 7873 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3076 3076
Size: 7873
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5683 278
Size: 53 83 186 936 4147 2468
Fragments arranged by size:
4147 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4968 5216 1937
Size: 3031 248 4594
Fragments arranged by size:
4594 3031 248
Acc65I G'GTAC_C
Cuts at: 5194 5194
Size: 7873
AclI AA'CG_TT
Cuts at: 1563 6000 6373 1563
Size: 4437 373 3063
Fragments arranged by size:
4437 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2429 2667 2712 3302 3366 4289 4685 4799
Size: 797 238 45 590 64 923 396 114
Cuts at: 4799 4844 5936 7320 7588 1632
Size: 45 1092 1384 268 1917
Fragments arranged by size:
1917 1384 1092 923 797 590 396 268
238 114 64 45 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7676
AflIII A'CryG_T
Cuts at: 1203 2103 4719 6772 7749 1203
Size: 900 2616 2053 977 1327
Fragments arranged by size:
2616 2053 1327 977 900
AgeI A'CCGG_T
Cuts at: 2913 6704 2913
Size: 3791 4082
AhdI GACnn_n'nnGTC
Cuts at: 4027 6602 4027
Size: 2575 5298
AleI CACnn'nnGTG
Cuts at: 1166 2345 3720 3722 1166
Size: 1179 1375 2 5317
Fragments arranged by size:
5317 1375 1179 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3068 1184
Size: 1884 5989
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2474 2506 2474
Size: 32 7841
AlwNI CAG_nnn'CTG
Cuts at: 2690 2743 3098 4095 4447 4663 5616 7188 2690
Size: 53 355 997 352 216 953 1572 3375
Fragments arranged by size:
3375 1572 997 953 355 352 216 53
ApaI G_GGCC'C
Cuts at: 4232 4690 4232
Size: 458 7415
ApaLI G'TGCA_C
Cuts at: 4984 5930 7086 4984
Size: 946 1156 5771
Fragments arranged by size:
5771 1156 946
ApoI r'AATT_y
Cuts at: 1493 2939 4409 4949 5182 5408 5560 7669 1493
Size: 1446 1470 540 233 226 152 2109 1697
Fragments arranged by size:
2109 1697 1470 1446 540 233 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6427 160
Size: 872 5395 1606
Fragments arranged by size:
5395 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7873
AvaI C'yCGr_G
Cuts at: 1870 1943 2713 3357 3947 4419 5198 1870
Size: 73 770 644 590 472 779 4545
Fragments arranged by size:
4545 779 770 644 590 472 73
BamHI G'GATC_C
Cuts at: 2075 5203 5570 2075
Size: 3128 367 4378
Fragments arranged by size:
4378 3128 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3064 3142 3620 3952 4817
Size: 325 217 627 1277 78 478 332 865
Cuts at: 4817 5194 6649 618
Size: 377 1455 1842
Fragments arranged by size:
1842 1455 1277 865 627 478 377 332
325 217 78
BanII G_rGCy'C
Cuts at: 1391 2865 3078 3663 3675 3996 4232 4690 4833
Size: 1474 213 585 12 321 236 458 143
Cuts at: 4833 5192 1391
Size: 359 4072
Fragments arranged by size:
4072 1474 585 458 359 321 236 213
143 12
BbeI G_GCGC'C
Cuts at: 3146 3956 4821 3146
Size: 810 865 6198
Fragments arranged by size:
6198 865 810
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 2260 2700 3787 5077 928
Size: 971 240 121 440 1087 1290 3724
Fragments arranged by size:
3724 1290 1087 971 440 240 121
BbvCI CC'TCA_GC
Cuts at: 2211 2478 2759 2901 3843 3891 2211
Size: 267 281 142 942 48 6193
Fragments arranged by size:
6193 942 281 267 142 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6064 6098 1903
Size: 34 50 34 4043 34 3678
Fragments arranged by size:
4043 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5765 6975 5765
Size: 1210 6663
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7873
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2168 4227 4358
Size: 107 122 71 631 605 496 2059 131
Cuts at: 4358 4815 6484 136
Size: 457 1669 1525
Fragments arranged by size:
2059 1669 1525 631 605 496 457 131
122 107 71
BglII A'GATC_T
Cuts at: 5010 7868 5010
Size: 2858 5015
BlpI GC'TnA_GC
Cuts at: 4832 5290 4832
Size: 458 7415
Bme1580I G_kGCm'C
Cuts at: 1790 2242 2891 3623 4013 4232 4690 4988 5137
Size: 452 649 732 390 219 458 298 149
Cuts at: 5137 5934 7090 1790
Size: 797 1156 2573
Fragments arranged by size:
2573 1156 797 732 649 458 452 390
298 219 149
BmgBI CAC'GTC
Cuts at: 1318 2520 1318
Size: 1202 6671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4636 5604 6562
Size: 416 53 83 399 24 3219 968 958
Cuts at: 6562 442
Size: 1753
Fragments arranged by size:
3219 1753 968 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7873
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2851 3199 3751 2851
Size: 348 552 6973
Fragments arranged by size:
6973 552 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 3392 3399 3704 3989 6533 1239
Size: 947 1206 7 305 285 2544 2579
Fragments arranged by size:
2579 2544 1206 947 305 285 7
Bpu10I CC'TnA_GC
Cuts at: 2211 2478 2498 2759 2901 3701 3843 3891 2211
Size: 267 20 261 142 800 142 48 6193
Fragments arranged by size:
6193 800 267 261 142 142 48 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3090 3435 4919 5998 6863 7161 3090
Size: 345 1484 1079 865 298 3802
Fragments arranged by size:
3802 1484 1079 865 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2922 3437 3611 4184 6536 882
Size: 617 1423 515 174 573 2352 2219
Fragments arranged by size:
2352 2219 1423 617 573 515 174
BsaAI yAC'GTr
Cuts at: 493 1204 2104 3121 5664 493
Size: 711 900 1017 2543 2702
Fragments arranged by size:
2702 2543 1017 900 711
BsaBI GATnn'nnATC
Cuts at: 7867 7867
Size: 7873
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3143 3953 4818 5680
Size: 53 83 186 936 1610 810 865 862
Cuts at: 5680 6062 6697 275
Size: 382 635 1451
Fragments arranged by size:
1610 1451 936 865 862 810 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2913 3570 6306 6704 6978 7125 7536 2913
Size: 657 2736 398 274 147 411 3250
Fragments arranged by size:
3250 2736 657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2474 2504 2852 2882 3890
Size: 30 165 30 1071 30 348 30 1008
Cuts at: 3890 3920 1178
Size: 30 5131
Fragments arranged by size:
5131 1071 1008 348 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 2743 2873 3725 3817 4370 4777 1186
Size: 1309 248 130 852 92 553 407 4282
Fragments arranged by size:
4282 1309 852 553 407 248 130 92
BseYI C'CCAG_C
Cuts at: 2259 2784 3146 3616 4689 4959 4989 7076 7572
Size: 525 362 470 1073 270 30 2087 496
Cuts at: 7572 2259
Size: 2560
Fragments arranged by size:
2560 2087 1073 525 496 470 362 270
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2176 2658 3038 3143 3938 1163
Size: 573 438 2 482 380 105 795 5098
Fragments arranged by size:
5098 795 573 482 438 380 105 2
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5323 6084 7112 1540
Size: 275 186 3322 761 1028 2301
Fragments arranged by size:
3322 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 3182 3663 3675 3717 4833 4988 5934 6019
Size: 857 481 12 42 1116 155 946 85
Cuts at: 6019 7090 2325
Size: 1071 3108
Fragments arranged by size:
3108 1116 1071 946 857 481 155 85
42 12
BsmI GAATG_Cn'
Cuts at: 5384 5477 5384
Size: 93 7780
BsmBI CGTCTCn'nnnn_
Cuts at: 4728 4759 4728
Size: 31 7842
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2242 2325 2865 2891 3078 3182 3623
Size: 399 452 83 540 26 187 104 441
Cuts at: 3623 3663 3675 3717 3996 4013 4232 4690 4833
Size: 40 12 42 279 17 219 458 143
Cuts at: 4833 4988 5137 5192 5934 6019 7090 1391
Size: 155 149 55 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 540 458 452 441 399
279 219 187 155 149 143 104 85
83 55 42 40 26 17 12
BspEI T'CCGG_A
Cuts at: 7536 7536
Size: 7873
BspHI T'CATG_A
Cuts at: 3265 3609 5762 7492 3265
Size: 344 2153 1730 3646
Fragments arranged by size:
3646 2153 1730 344
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3060 3076 3990 4713 5228 844
Size: 2216 16 914 723 515 3489
Fragments arranged by size:
3489 2216 914 723 515 16
BsrBI CCG'CTC
Cuts at: 1394 1823 3631 4744 5760 1394
Size: 429 1808 1113 1016 3507
Fragments arranged by size:
3507 1808 1113 1016 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2851 3648 6368 6542 65
Size: 1183 1603 797 2720 174 1396
Fragments arranged by size:
2720 1603 1396 1183 797 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2913 3348 5139 6517 6704 1700
Size: 110 1103 435 1791 1378 187 2869
Fragments arranged by size:
2869 1791 1378 1103 435 187 110
BsrGI T'GTAC_A
Cuts at: 96 4080 96
Size: 3984 3889
BssHII G'CGCG_C
Cuts at: 1925 3820 1925
Size: 1895 5978
BssSI C'ACGA_G
Cuts at: 5933 6945 5933
Size: 1012 6861
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7873
BstBI TT'CG_AA
Cuts at: 2532 6677 2532
Size: 4145 3728
BstEII G'GTnAC_C
Cuts at: 1184 4363 1184
Size: 3179 4694
BstXI CCAn_nnnn'nTGG
Cuts at: 2831 3154 3229 3242 3776 2831
Size: 323 75 13 534 6928
Fragments arranged by size:
6928 534 323 75 13
BstYI r'GATC_y
Cuts at: 1972 2075 2997 3327 4312 4680 5010 5203 5570
Size: 103 922 330 985 368 330 193 367
Cuts at: 5570 5955 5972 7424 7510 7522 7868 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 985 922 385 368 367 346
330 330 193 103 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3184 3936 3184
Size: 752 7121
BtgI C'CryG_G
Cuts at: 513 1065 2182 3028 3624 3996 4180 4461 513
Size: 552 1117 846 596 372 184 281 3925
Fragments arranged by size:
3925 1117 846 596 552 372 281 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7134
Fragments arranged by size:
7134 683 56
BtsI GCAGTG_nn'
Cuts at: 4972 5389 6183 6203 4972
Size: 417 794 20 6642
Fragments arranged by size:
6642 794 417 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7873
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7838
DraI TTT'AAA
Cuts at: 5177 5177
Size: 7873
DraIII CAC_nnn'GTG
Cuts at: 2154 4186 2154
Size: 2032 5841
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2120 2702 3418 5057 6880 809
Size: 507 804 582 716 1639 1823 1802
Fragments arranged by size:
1823 1802 1639 804 716 582 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2772 4254 4608
Size: 54 1425 50 275 18 942 1482 354
Cuts at: 4608 5320 6209 7838 8
Size: 712 889 1629 43
Fragments arranged by size:
1629 1482 1425 942 889 712 354 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5320 1537
Size: 275 3508 4090
Fragments arranged by size:
4090 3508 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3668 5803 1353
Size: 2315 2135 3423
Fragments arranged by size:
3423 2315 2135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3680 4021 6460 6834 6980 387
Size: 1064 138 2091 341 2439 374 146 1280
Fragments arranged by size:
2439 2091 1280 1064 374 341 146 138
EcoICRI GAG'CTC
Cuts at: 3661 3673 4831 3661
Size: 12 1158 6703
Fragments arranged by size:
6703 1158 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2429 2667 2712 3302 3366 3392
Size: 393 554 243 238 45 590 64 26
Cuts at: 3392 3399 3704 3989 4289 4685 4799 4844 5936
Size: 7 305 285 300 396 114 45 1092
Cuts at: 5936 6533 7320 7588 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1092 787 597 590 554 396 393
305 300 285 268 243 238 114 64
45 45 26 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3251 3507 3878 3251
Size: 256 371 7246
Fragments arranged by size:
7246 371 256
EcoO109I rG'GnC_Cy
Cuts at: 2512 3871 4092 4228 4630 4660 4939 2512
Size: 1359 221 136 402 30 279 5446
Fragments arranged by size:
5446 1359 402 279 221 136 30
EcoRI G'AATT_C
Cuts at: 2939 5182 5560 2939
Size: 2243 378 5252
Fragments arranged by size:
5252 2243 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3679 5101 3679
Size: 1422 6451
FspI TGC'GCA
Cuts at: 4498 6379 4498
Size: 1881 5992
HaeII r_GCGC'y
Cuts at: 1678 2163 3146 3784 3956 4645 4821 7020 1678
Size: 485 983 638 172 689 176 2199 2531
Fragments arranged by size:
2531 2199 983 689 638 485 176 172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2609 2641 2852 2884 3971
Size: 32 135 32 1037 32 211 32 1087
Cuts at: 3971 4003 4013 4045 4527 4559 4580 4612 6514
Size: 32 10 32 482 32 21 32 1902
Cuts at: 6514 6546 6588 6620 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1902 1087 1037 482 211 135 42
32 32 32 32 32 32 32 32
32 32 21 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2338 5217 5463 669
Size: 988 281 400 2879 246 3079
Fragments arranged by size:
3079 2879 988 400 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7873
HpaI GTT'AAC
Cuts at: 5463 5463
Size: 7873
KasI G'GCGC_C
Cuts at: 3142 3952 4817 3142
Size: 810 865 6198
Fragments arranged by size:
6198 865 810
KpnI G_GTAC'C
Cuts at: 5198 5198
Size: 7873
MfeI C'AATT_G
Cuts at: 5472 5472
Size: 7873
MluI A'CGCG_T
Cuts at: 4719 7749 4719
Size: 3030 4843
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 2916 3615 4924 6987 7171 1305
Size: 993 618 699 1309 2063 184 2007
Fragments arranged by size:
2063 2007 1309 993 699 618 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6051
Fragments arranged by size:
6051 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2345 3720 3722 3774 5831 6190 6349
Size: 648 1179 1375 2 52 2057 359 159
Cuts at: 6349 518
Size: 2042
Fragments arranged by size:
2057 2042 1375 1179 648 359 159 52
2
MspA1I CmG'CkG
Cuts at: 1606 2355 2650 2788 3682 3998 4777 5130 5289
Size: 749 295 138 894 316 779 353 159
Cuts at: 5289 5966 7114 7359 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 894 779 749 677 353 316
295 245 159 138
NaeI GCC'GGC
Cuts at: 5141 5141
Size: 7873
NarI GG'CG_CC
Cuts at: 3143 3953 4818 3143
Size: 810 865 6198
Fragments arranged by size:
6198 865 810
NcoI C'CATG_G
Cuts at: 513 1065 2182 3028 4461 513
Size: 552 1117 846 1433 3925
Fragments arranged by size:
3925 1433 1117 846 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7873
NgoMIV G'CCGG_C
Cuts at: 5139 5139
Size: 7873
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7873
NotI GC'GGCC_GC
Cuts at: 5320 5320
Size: 7873
NspI r_CATG'y
Cuts at: 1132 1229 4739 4967 5231 6776 1132
Size: 97 3510 228 264 1545 2229
Fragments arranged by size:
3510 2229 1545 264 228 97
PasI CC'CwG_GG
Cuts at: 1348 3475 3541 3958 4369 1348
Size: 2127 66 417 411 4852
Fragments arranged by size:
4852 2127 417 411 66
PciI A'CATG_T
Cuts at: 6772 6772
Size: 7873
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4186 5107 1108
Size: 3078 921 3874
Fragments arranged by size:
3874 3078 921
PfoI T'CCnGG_A
Cuts at: 2469 4197 2469
Size: 1728 6145
PmeI GTTT'AAAC
Cuts at: 5177 5177
Size: 7873
PmlI CAC'GTG
Cuts at: 3121 3121
Size: 7873
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2474 2506 5942 5974 7455 7487 2474
Size: 32 3436 32 1481 32 2860
Fragments arranged by size:
3436 2860 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4092 4630 4092
Size: 538 7335
PshAI GACnn'nnGTC
Cuts at: 1613 2333 1613
Size: 720 7153
PsiI TTA'TAA
Cuts at: 5443 5443
Size: 7873
PspOMI G'GGCC_C
Cuts at: 4228 4686 4228
Size: 458 7415
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3905 3937 3905
Size: 32 7841
PstI C_TGCA'G
Cuts at: 830 2424 2511 2798 3073 3252 3879 4278 4443
Size: 1594 87 287 275 179 627 399 165
Cuts at: 4443 4655 4710 5225 830
Size: 212 55 515 3478
Fragments arranged by size:
3478 1594 627 515 399 287 275 212
179 165 87 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7873
PvuII CAG'CTG
Cuts at: 2788 4777 2788
Size: 1989 5884
SacI G_AGCT'C
Cuts at: 3663 3675 4833 3663
Size: 12 1158 6703
Fragments arranged by size:
6703 1158 12
SacII CC_GC'GG
Cuts at: 3999 3999
Size: 7873
SalI G'TCGA_C
Cuts at: 1936 5215 1936
Size: 3279 4594
SapI GCTCTTCn'nnn_
Cuts at: 3668 3668
Size: 7873
SbfI CC_TGCA'GG
Cuts at: 2511 5225 2511
Size: 2714 5159
ScaI AGT'ACT
Cuts at: 2447 3205 6121 7833 2447
Size: 758 2916 1712 2487
Fragments arranged by size:
2916 2487 1712 758
SexAI A'CCwGG_T
Cuts at: 4446 4446
Size: 7873
SfcI C'TryA_G
Cuts at: 826 1045 2420 2507 2645 2794 3069 3171 3248
Size: 219 1375 87 138 149 275 102 77
Cuts at: 3248 3875 4274 4439 4651 4706 5221 6356 7037
Size: 627 399 165 212 55 515 1135 681
Cuts at: 7037 7228 826
Size: 191 1471
Fragments arranged by size:
1471 1375 1135 681 627 515 399 275
219 212 191 165 149 138 102 87
77 55
SfoI GGC'GCC
Cuts at: 3144 3954 4819 3144
Size: 810 865 6198
Fragments arranged by size:
6198 865 810
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7873
SgrAI Cr'CCGG_yG
Cuts at: 1700 5139 1700
Size: 3439 4434
SmaI CCC'GGG
Cuts at: 1872 3359 4421 5200 1872
Size: 1487 1062 779 4545
Fragments arranged by size:
4545 1487 1062 779
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3105 3450 4934 5977 6878 7140
Size: 197 926 1162 345 1484 1043 901 262
Cuts at: 7140 820
Size: 1553
Fragments arranged by size:
1553 1484 1162 1043 926 901 345 262
197
SnaBI TAC'GTA
Cuts at: 493 5664 493
Size: 5171 2702
SpeI A'CTAG_T
Cuts at: 152 2126 152
Size: 1974 5899
SphI G_CATG'C
Cuts at: 1132 5231 1132
Size: 4099 3774
SspI AAT'ATT
Cuts at: 5 52 5797 5
Size: 47 5745 2081
Fragments arranged by size:
5745 2081 47
StuI AGG'CCT
Cuts at: 1517 2087 3014 3427 4473 1517
Size: 570 927 413 1046 4917
Fragments arranged by size:
4917 1046 927 570 413
StyI C'CwwG_G
Cuts at: 513 1065 2182 3028 3235 4122 4222 4257 4461
Size: 552 1117 846 207 887 100 35 204
Cuts at: 4461 4483 5312 513
Size: 22 829 3074
Fragments arranged by size:
3074 1117 887 846 829 552 207 204
100 35 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2309 3316 3405 3898 5003 5900 5917
Size: 1255 729 1007 89 493 1105 897 17
Cuts at: 5917 6070 6255 6700 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1105 1007 897 729 493 445
185 153 89 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2445 3203 4074
Size: 274 80 33 51 1443 468 758 871
Cuts at: 4074 4080 6119 7831 96
Size: 6 2039 1712 138
Fragments arranged by size:
2039 1712 1443 871 758 468 274 138
80 51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2221 2253 3890 3922 7833 7865 2221
Size: 32 1637 32 3911 32 2229
Fragments arranged by size:
3911 2229 1637 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7873
XbaI T'CTAG_A
Cuts at: 5209 5209
Size: 7873
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3095 2011
Size: 1084 6789
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7873
XmaI C'CCGG_G
Cuts at: 1870 3357 4419 5198 1870
Size: 1487 1062 779 4545
Fragments arranged by size:
4545 1487 1062 779
XmnI GAAnn'nnTTC
Cuts at: 4139 4430 5165 6002 4139
Size: 291 735 837 6010
Fragments arranged by size:
6010 837 735 291
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5681 276
Size: 53 83 186 936 4147 2468
Fragments arranged by size:
4147 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PspXI RsrII SanDI
SfiI SrfI SwaI