Restriction map for
pFN21AA0351
(Circular) (Six-base) MAPSORT of: pFN21AA0351.seq Check: 9331 from: 1 to: 6376
pFN21AA0351 6376 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4186 278
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3719 1937
Size: 1782 4594
Acc65I G'GTAC_C
Cuts at: 3649 3697 3649
Size: 48 6328
AclI AA'CG_TT
Cuts at: 1563 2274 4503 4876 1563
Size: 711 2229 373 3063
Fragments arranged by size:
3063 2229 711 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2449 2606 2810 3222 4439 5823 6091 1632
Size: 817 157 204 412 1217 1384 268 1917
Fragments arranged by size:
1917 1384 1217 817 412 268 204 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6179
AflIII A'CryG_T
Cuts at: 1203 5275 6252 1203
Size: 4072 977 1327
Fragments arranged by size:
4072 1327 977
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 5105 5105
Size: 6376
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6376
AlwNI CAG_nnn'CTG
Cuts at: 3378 4119 5691 3378
Size: 741 1572 4063
Fragments arranged by size:
4063 1572 741
ApaLI G'TGCA_C
Cuts at: 4433 5589 4433
Size: 1156 5220
ApoI r'AATT_y
Cuts at: 1493 2328 3685 3911 4063 6172 1493
Size: 835 1357 226 152 2109 1697
Fragments arranged by size:
2109 1697 1357 835 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2183 4930 160
Size: 872 1151 2747 1606
Fragments arranged by size:
2747 1606 1151 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 3515 3701 1870
Size: 73 1572 186 4545
Fragments arranged by size:
4545 1572 186 73
AvrII C'CTAG_G
Cuts at: 3031 3196 3031
Size: 165 6211
BamHI G'GATC_C
Cuts at: 3395 3706 4073 3395
Size: 311 367 5698
Fragments arranged by size:
5698 367 311
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3105 3649 3697 5152 618
Size: 325 217 627 1318 544 48 1455 1842
Fragments arranged by size:
1842 1455 1318 627 544 325 217 48
BanII G_rGCy'C
Cuts at: 1391 2488 2817 3215 3695 1391
Size: 1097 329 398 480 4072
Fragments arranged by size:
4072 1097 480 398 329
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2125 2838 3228 928
Size: 971 226 713 390 4076
Fragments arranged by size:
4076 971 713 390 226
BbvCI CC'TCA_GC
Cuts at: 2369 2369
Size: 6376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4567 4601 1903
Size: 34 2630 34 3678
Fragments arranged by size:
3678 2630 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4268 5478 4268
Size: 1210 5166
BclI T'GATC_A
Cuts at: 1548 2752 1548
Size: 1204 5172
BfrBI ATG'CAT
Cuts at: 3626 3626
Size: 6376
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3258 4987 136
Size: 107 122 71 631 605 1586 1729 1525
Fragments arranged by size:
1729 1586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2905 6371 2905
Size: 3466 2910
BlpI GC'TnA_GC
Cuts at: 3793 3793
Size: 6376
Bme1580I G_kGCm'C
Cuts at: 1790 3300 3437 4437 5593 1790
Size: 1510 137 1000 1156 2573
Fragments arranged by size:
2573 1510 1156 1000 137
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6376
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3388 4107 5065
Size: 416 53 83 399 24 1971 719 958
Cuts at: 5065 442
Size: 1753
Fragments arranged by size:
1971 1753 958 719 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2034 2172 1056
Size: 978 138 5260
Fragments arranged by size:
5260 978 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2801 2985 5036 1239
Size: 866 696 184 2051 2579
Fragments arranged by size:
2579 2051 866 696 184
Bpu10I CC'TnA_GC
Cuts at: 2369 2369
Size: 6376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3320 4501 5366 5664 3320
Size: 1181 865 298 4032
Fragments arranged by size:
4032 1181 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5039 882
Size: 617 3540 2219
Fragments arranged by size:
3540 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4167 493
Size: 711 2963 2702
Fragments arranged by size:
2963 2702 711
BsaBI GATnn'nnATC
Cuts at: 6370 6370
Size: 6376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4183 4565 5200 275
Size: 53 83 186 936 2650 382 635 1451
Fragments arranged by size:
2650 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2930 4809 5207 5481 5628 6039 2930
Size: 1879 398 274 147 411 3267
Fragments arranged by size:
3267 1879 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6151
Fragments arranged by size:
6151 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 3157 3160 1186
Size: 988 983 3 4402
Fragments arranged by size:
4402 988 983 3
BseYI C'CCAG_C
Cuts at: 5579 6075 5579
Size: 496 5880
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2798 3518 1163
Size: 573 1062 720 4021
Fragments arranged by size:
4021 1062 720 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3826 4587 5615 1540
Size: 275 186 1825 761 1028 2301
Fragments arranged by size:
2301 1825 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2458 2817 3215 3522 4437 4522 5593 2458
Size: 359 398 307 915 85 1071 3241
Fragments arranged by size:
3241 1071 915 398 359 307 85
BsiWI C'GTAC_G
Cuts at: 2797 2797
Size: 6376
BsmI GAATG_Cn'
Cuts at: 3887 3980 3887
Size: 93 6283
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2458 2488 2817 3215 3300 3437 3522
Size: 399 668 30 329 398 85 137 85
Cuts at: 3522 3695 4437 4522 5593 1391
Size: 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 668 399 398 329 173
137 85 85 85 30
BspEI T'CCGG_A
Cuts at: 6039 6039
Size: 6376
BspHI T'CATG_A
Cuts at: 4265 5995 4265
Size: 1730 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2903 3661 3731 844
Size: 2059 758 70 3489
Fragments arranged by size:
3489 2059 758 70
BsrBI CCG'CTC
Cuts at: 1394 1823 4263 1394
Size: 429 2440 3507
Fragments arranged by size:
3507 2440 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3565 4871 5045 65
Size: 1183 2317 1306 174 1396
Fragments arranged by size:
2317 1396 1306 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3257 5020 5207 1700
Size: 110 1447 1763 187 2869
Fragments arranged by size:
2869 1763 1447 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 4465
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6376
BssSI C'ACGA_G
Cuts at: 3364 4436 5448 3364
Size: 1072 1012 4292
Fragments arranged by size:
4292 1072 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2657 794
Size: 1863 4513
BstBI TT'CG_AA
Cuts at: 2716 5180 2716
Size: 2464 3912
BstEII G'GTnAC_C
Cuts at: 1184 2041 1184
Size: 857 5519
BstYI r'GATC_y
Cuts at: 1972 2690 2905 3395 3706 4073 4458 4475 5927
Size: 718 215 490 311 367 385 17 1452
Cuts at: 5927 6013 6025 6371 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 718 490 385 367 346 311
215 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2662 3645 2662
Size: 983 5393
BtgI C'CryG_G
Cuts at: 513 1065 3303 513
Size: 552 2238 3586
Fragments arranged by size:
3586 2238 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5637
Fragments arranged by size:
5637 683 56
BtsI GCAGTG_nn'
Cuts at: 3892 4686 4706 3892
Size: 794 20 5562
Fragments arranged by size:
5562 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6341
DraI TTT'AAA
Cuts at: 2203 2349 2493 3680 2203
Size: 146 144 1187 4899
Fragments arranged by size:
4899 1187 146 144
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3122 5383 809
Size: 507 1806 2261 1802
Fragments arranged by size:
2261 1806 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3823 4712 6341
Size: 54 1425 50 275 18 1993 889 1629
Cuts at: 6341 8
Size: 43
Fragments arranged by size:
1993 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3823 1537
Size: 275 2011 4090
Fragments arranged by size:
4090 2011 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2805 2899 3014 3222 4306 1353
Size: 1452 94 115 208 1084 3423
Fragments arranged by size:
3423 1452 1084 208 115 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3361 4963 5337 5483 387
Size: 1064 138 1772 1602 374 146 1280
Fragments arranged by size:
1772 1602 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2815 3213 2815
Size: 398 5978
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2449 2606 2801 2810 2985 3222
Size: 393 473 344 157 195 9 175 237
Cuts at: 3222 4439 5036 5823 6091 1239
Size: 1217 597 787 268 1524
Fragments arranged by size:
1524 1217 787 597 473 393 344 268
237 195 175 157 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2661 3335 3552 2661
Size: 674 217 5485
Fragments arranged by size:
5485 674 217
EcoO109I rG'GnC_Cy
Cuts at: 2915 3151 3311 2915
Size: 236 160 5980
Fragments arranged by size:
5980 236 160
EcoRI G'AATT_C
Cuts at: 3685 4063 3685
Size: 378 5998
EcoRV GAT'ATC
Cuts at: 3529 3529
Size: 6376
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 6376
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 5523 1678
Size: 3845 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2005 2037 3387 3419 5017
Size: 32 135 32 433 32 1350 32 1598
Cuts at: 5017 5049 5091 5123 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1598 1350 433 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3720 3966 669
Size: 988 281 1782 246 3079
Fragments arranged by size:
3079 1782 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6376
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KpnI G_GTAC'C
Cuts at: 3653 3701 3653
Size: 48 6328
MfeI C'AATT_G
Cuts at: 3975 3975
Size: 6376
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5490 5674 1305
Size: 4185 184 2007
Fragments arranged by size:
4185 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4554
Fragments arranged by size:
4554 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2432 3618 4334 4693 4852 518
Size: 648 1266 1186 716 359 159 2042
Fragments arranged by size:
2042 1266 1186 716 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2232 3293 3502 3538 3792 4469 5617 5862
Size: 626 1061 209 36 254 677 1148 245
Cuts at: 5862 1606
Size: 2120
Fragments arranged by size:
2120 1148 1061 677 626 254 245 209
36
NcoI C'CATG_G
Cuts at: 513 1065 3303 513
Size: 552 2238 3586
Fragments arranged by size:
3586 2238 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6376
NheI G'CTAG_C
Cuts at: 1052 2030 2168 1052
Size: 978 138 5260
Fragments arranged by size:
5260 978 138
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NsiI A_TGCA'T
Cuts at: 3628 3628
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 3630 3734 5279 1132
Size: 97 2401 104 1545 2229
Fragments arranged by size:
2401 2229 1545 104 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6376
PciI A'CATG_T
Cuts at: 5275 5275
Size: 6376
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3378 1108
Size: 2270 4106
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4445 4477 5958 5990 4445
Size: 32 1481 32 4831
Fragments arranged by size:
4831 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2915 2915
Size: 6376
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6376
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PstI C_TGCA'G
Cuts at: 830 2747 3728 830
Size: 1917 981 3478
Fragments arranged by size:
3478 1917 981
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6376
PvuII CAG'CTG
Cuts at: 2232 3293 3502 3538 2232
Size: 1061 209 36 5070
Fragments arranged by size:
5070 1061 209 36
SacI G_AGCT'C
Cuts at: 2817 3215 2817
Size: 398 5978
SalI G'TCGA_C
Cuts at: 1936 3718 1936
Size: 1782 4594
SapI GCTCTTCn'nnn_
Cuts at: 2805 3014 2805
Size: 209 6167
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SexAI A'CCwGG_T
Cuts at: 2301 3371 2301
Size: 1070 5306
SfcI C'TryA_G
Cuts at: 826 1045 2743 3724 4859 5540 5731 826
Size: 219 1698 981 1135 681 191 1471
Fragments arranged by size:
1698 1471 1135 981 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 3703 1872
Size: 1831 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3335 4480 5381 5643 820
Size: 197 926 1392 1145 901 262 1553
Fragments arranged by size:
1553 1392 1145 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4167 493
Size: 3674 2702
SpeI A'CTAG_T
Cuts at: 152 2887 152
Size: 2735 3641
SphI G_CATG'C
Cuts at: 1132 3734 1132
Size: 2602 3774
SspI AAT'ATT
Cuts at: 5 52 2710 4300 5
Size: 47 2658 1590 2081
Fragments arranged by size:
2658 2081 1590 47
StuI AGG'CCT
Cuts at: 1517 3643 1517
Size: 2126 4250
StyI C'CwwG_G
Cuts at: 513 1065 2898 3031 3196 3303 3815 513
Size: 552 1833 133 165 107 512 3074
Fragments arranged by size:
3074 1833 552 512 165 133 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3537 4403 4420 4573 4758 5203 325
Size: 1255 1957 866 17 153 185 445 1498
Fragments arranged by size:
1957 1498 1255 866 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 3408 4622
Size: 274 80 33 51 1443 30 1401 1214
Cuts at: 4622 6334 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1401 1214 274 138 80 51
33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2988 3020 3440 3472 6336 6368 2988
Size: 32 420 32 2864 32 2996
Fragments arranged by size:
2996 2864 420 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3122 1316
Size: 1806 4570
XbaI T'CTAG_A
Cuts at: 2401 3712 2401
Size: 1311 5065
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 3701 1870
Size: 1831 4545
XmnI GAAnn'nnTTC
Cuts at: 4505 4505
Size: 6376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4184 276
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BplI
BsmBI BstXI BstZ17I DraIII FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI
XcmI