Restriction map for
pFN21AA0350
(Circular) (Six-base) MAPSORT of: pFN21AA0350.seq Check: 6728 from: 1 to: 7864
pFN21AA0350 7864 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2793 2859 3422 4270 4563 2793
Size: 66 563 848 293 6094
Fragments arranged by size:
6094 848 563 293 66
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5674 278
Size: 53 83 186 936 4138 2468
Fragments arranged by size:
4138 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5207 1937
Size: 3270 4594
Acc65I G'GTAC_C
Cuts at: 5185 5185
Size: 7864
AclI AA'CG_TT
Cuts at: 1563 3189 5991 6364 1563
Size: 1626 2802 373 3063
Fragments arranged by size:
3063 2802 1626 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3027 3731 4530 5927 7311 7579 1632
Size: 1395 704 799 1397 1384 268 1917
Fragments arranged by size:
1917 1397 1395 1384 799 704 268
AfeI AGC'GCT
Cuts at: 4613 4613
Size: 7864
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7667
AflIII A'CryG_T
Cuts at: 1203 2280 2880 3840 6763 7740 1203
Size: 1077 600 960 2923 977 1327
Fragments arranged by size:
2923 1327 1077 977 960 600
AgeI A'CCGG_T
Cuts at: 6695 6695
Size: 7864
AhdI GACnn_n'nnGTC
Cuts at: 6593 6593
Size: 7864
AleI CACnn'nnGTG
Cuts at: 1166 3365 1166
Size: 2199 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3383 4478 1184
Size: 2199 1095 4570
Fragments arranged by size:
4570 2199 1095
AlwNI CAG_nnn'CTG
Cuts at: 2296 4426 4555 4752 5607 7179 2296
Size: 2130 129 197 855 1572 2981
Fragments arranged by size:
2981 2130 1572 855 197 129
ApaI G_GGCC'C
Cuts at: 3222 3222
Size: 7864
ApaLI G'TGCA_C
Cuts at: 3755 5921 7077 3755
Size: 2166 1156 4542
Fragments arranged by size:
4542 2166 1156
ApoI r'AATT_y
Cuts at: 1493 2374 2393 5173 5399 5551 7660 1493
Size: 881 19 2780 226 152 2109 1697
Fragments arranged by size:
2780 2109 1697 881 226 152 19
AseI AT'TA_AT
Cuts at: 160 1032 6418 160
Size: 872 5386 1606
Fragments arranged by size:
5386 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7864
AvaI C'yCGr_G
Cuts at: 1870 1943 3119 4077 5189 1870
Size: 73 1176 958 1112 4545
Fragments arranged by size:
4545 1176 1112 958 73
BamHI G'GATC_C
Cuts at: 3547 5194 5561 3547
Size: 1647 367 5850
Fragments arranged by size:
5850 1647 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4581 4639 4766 5150 5185
Size: 325 217 627 2794 58 127 384 35
Cuts at: 5185 6640 618
Size: 1455 1842
Fragments arranged by size:
2794 1842 1455 627 384 325 217 127
58 35
BanII G_rGCy'C
Cuts at: 1391 3222 4727 5115 5183 1391
Size: 1831 1505 388 68 4072
Fragments arranged by size:
4072 1831 1505 388 68
BbeI G_GCGC'C
Cuts at: 4585 4585
Size: 7864
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2956 3246 3605 3831 3995 928
Size: 971 1057 290 359 226 164 4797
Fragments arranged by size:
4797 1057 971 359 290 226 164
BbvCI CC'TCA_GC
Cuts at: 5157 5157
Size: 7864
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2723 2757 3937 3971 4118 4152 4289
Size: 34 786 34 1180 34 147 34 137
Cuts at: 4289 4323 5111 5145 6055 6089 1903
Size: 34 788 34 910 34 3678
Fragments arranged by size:
3678 1180 910 788 786 147 137 34
34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5756 6966 5756
Size: 1210 6654
BclI T'GATC_A
Cuts at: 1548 2808 3321 4092 4149 1548
Size: 1260 513 771 57 5263
Fragments arranged by size:
5263 1260 771 513 57
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3251 4731 5070
Size: 107 122 71 631 605 1579 1480 339
Cuts at: 5070 6475 136
Size: 1405 1525
Fragments arranged by size:
1579 1525 1480 1405 631 605 339 122
107 71
BglII A'GATC_T
Cuts at: 7859 7859
Size: 7864
BlpI GC'TnA_GC
Cuts at: 3695 5009 5139 5281 3695
Size: 1314 130 142 6278
Fragments arranged by size:
6278 1314 142 130
Bme1580I G_kGCm'C
Cuts at: 1790 3222 3759 4312 5153 5925 7081 1790
Size: 1432 537 553 841 772 1156 2573
Fragments arranged by size:
2573 1432 1156 841 772 553 537
BmgBI CAC'GTC
Cuts at: 1318 3754 1318
Size: 2436 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4654 5033 5595
Size: 416 53 83 399 24 3237 379 562
Cuts at: 5595 6553 442
Size: 958 1753
Fragments arranged by size:
3237 1753 958 562 416 399 379 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3623 1056
Size: 2567 5297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2243 2912 3710 3791 4355 4782 6524 1239
Size: 1004 669 798 81 564 427 1742 2579
Fragments arranged by size:
2579 1742 1004 798 669 564 427 81
Bpu10I CC'TnA_GC
Cuts at: 4836 5157 4836
Size: 321 7543
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 3152 5989 6854 7152 2066
Size: 1086 2837 865 298 2778
Fragments arranged by size:
2837 2778 1086 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2144 2330 3166 6527 882
Size: 617 645 186 836 3361 2219
Fragments arranged by size:
3361 2219 836 645 617 186
BsaAI yAC'GTr
Cuts at: 493 1204 2281 2371 2881 5655 493
Size: 711 1077 90 510 2774 2702
Fragments arranged by size:
2774 2702 1077 711 510 90
BsaBI GATnn'nnATC
Cuts at: 2177 3320 7858 2177
Size: 1143 4538 2183
Fragments arranged by size:
4538 2183 1143
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3243 3906 4582 5671
Size: 53 83 186 936 1710 663 676 1089
Cuts at: 5671 6053 6688 275
Size: 382 635 1451
Fragments arranged by size:
1710 1451 1089 936 676 663 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2133 6297 6695 6969 7116 7527 2133
Size: 4164 398 274 147 411 2470
Fragments arranged by size:
4164 2470 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2081 1178
Size: 30 165 30 648 30 6961
Fragments arranged by size:
6961 648 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 2744 2925 3224 3407 3506 4097 4525
Size: 1240 318 181 299 183 99 591 428
Cuts at: 4525 1186
Size: 4525
Fragments arranged by size:
4525 1240 591 428 318 299 183 181
99
BseYI C'CCAG_C
Cuts at: 2025 3083 3657 4239 4727 4895 4946 7067 7563
Size: 1058 574 582 488 168 51 2121 496
Cuts at: 7563 2025
Size: 2326
Fragments arranged by size:
2326 2121 1058 582 574 496 488 168
51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2612 2738 2771 3389 4628 5089 1163
Size: 573 876 126 33 618 1239 461 3938
Fragments arranged by size:
3938 1239 876 618 573 461 126 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2921 3594 5314 6075 7103 1540
Size: 275 186 920 673 1720 761 1028 2301
Fragments arranged by size:
2301 1720 1028 920 761 673 275 186
BsiHKAI G_wGCw'C
Cuts at: 2499 2775 2841 3428 3510 3759 4745 5925 6010
Size: 276 66 587 82 249 986 1180 85
Cuts at: 6010 7081 2499
Size: 1071 3282
Fragments arranged by size:
3282 1180 1071 986 587 276 249 85
82 66
BsmI GAATG_Cn'
Cuts at: 3087 5375 5468 3087
Size: 2288 93 5483
Fragments arranged by size:
5483 2288 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2499 2775 2841 3222 3428 3510 3759
Size: 399 709 276 66 381 206 82 249
Cuts at: 3759 4312 4727 4745 5115 5153 5183 5925 6010
Size: 553 415 18 370 38 30 742 85
Cuts at: 6010 7081 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 709 553 415 399 381
370 276 249 206 85 82 66 38
30 18
BspEI T'CCGG_A
Cuts at: 7527 7527
Size: 7864
BspHI T'CATG_A
Cuts at: 3642 3744 3897 5753 7483 3642
Size: 102 153 1856 1730 4023
Fragments arranged by size:
4023 1856 1730 153 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2148 2793 2859 3422 4270 4563 5219 844
Size: 1304 645 66 563 848 293 656 3489
Fragments arranged by size:
3489 1304 848 656 645 563 293 66
BsrBI CCG'CTC
Cuts at: 1394 1823 2016 3124 5751 1394
Size: 429 193 1108 2627 3507
Fragments arranged by size:
3507 2627 1108 429 193
BsrDI GCAATG_nn'
Cuts at: 65 1248 3437 6359 6533 65
Size: 1183 2189 2922 174 1396
Fragments arranged by size:
2922 2189 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2935 5069 5092 6508 6695 1700
Size: 110 1125 2134 23 1416 187 2869
Fragments arranged by size:
2869 2134 1416 1125 187 110 23
BsrGI T'GTAC_A
Cuts at: 96 2517 4142 96
Size: 2421 1625 3818
Fragments arranged by size:
3818 2421 1625
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7864
BssSI C'ACGA_G
Cuts at: 2333 5924 6936 2333
Size: 3591 1012 3261
Fragments arranged by size:
3591 3261 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3093 4276 794
Size: 2299 1183 4382
Fragments arranged by size:
4382 2299 1183
BstBI TT'CG_AA
Cuts at: 6668 6668
Size: 7864
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7864
BstXI CCAn_nnnn'nTGG
Cuts at: 3665 3886 3665
Size: 221 7643
BstYI r'GATC_y
Cuts at: 1972 2172 3547 3672 4114 5194 5561 5946 5963
Size: 200 1375 125 442 1080 367 385 17
Cuts at: 5963 7415 7501 7513 7859 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1375 1080 442 385 367 346
200 125 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2041 3384 4653 4923 5115 513
Size: 552 976 1343 1269 270 192 3262
Fragments arranged by size:
3262 1343 1269 976 552 270 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3993 4396 487
Size: 683 56 2767 403 3955
Fragments arranged by size:
3955 2767 683 403 56
BtsI GCAGTG_nn'
Cuts at: 4034 4750 5380 6174 6194 4034
Size: 716 630 794 20 5704
Fragments arranged by size:
5704 794 716 630 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7864
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3274 3309 529
Size: 35 2710 35 5084
Fragments arranged by size:
5084 2710 35 35
DraI TTT'AAA
Cuts at: 5168 5168
Size: 7864
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6871 809
Size: 507 5555 1802
Fragments arranged by size:
5555 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 2918 3351
Size: 54 1425 50 275 18 181 907 433
Cuts at: 3351 3591 4428 4908 5311 6200 7829 8
Size: 240 837 480 403 889 1629 43
Fragments arranged by size:
1629 1425 907 889 837 480 433 403
275 240 181 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2918 3591 5311 1537
Size: 275 1106 673 1720 4090
Fragments arranged by size:
4090 1720 1106 673 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2870 3300 5118 5794 1353
Size: 1517 430 1818 676 3423
Fragments arranged by size:
3423 1818 1517 676 430
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4702 6451 6825 6971 387
Size: 1064 138 3113 1749 374 146 1280
Fragments arranged by size:
3113 1749 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2912 3027 3710 3731 3791 4355
Size: 393 611 669 115 683 21 60 564
Cuts at: 4355 4530 4782 5927 6524 7311 7579 1239
Size: 175 252 1145 597 787 268 1524
Fragments arranged by size:
1524 1145 787 683 669 611 597 564
393 268 252 175 115 60 21
EcoNI CCTnn'n_nnAGG
Cuts at: 4268 4268
Size: 7864
EcoO109I rG'GnC_Cy
Cuts at: 3218 4087 3218
Size: 869 6995
EcoRI G'AATT_C
Cuts at: 5173 5551 5173
Size: 378 7486
FspI TGC'GCA
Cuts at: 6370 6370
Size: 7864
HaeII r_GCGC'y
Cuts at: 1678 3405 4426 4585 4615 7011 1678
Size: 1727 1021 159 30 2396 2531
Fragments arranged by size:
2531 2396 1727 1021 159 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 6505 6537 6579 6611 1373
Size: 32 135 32 4933 32 42 32 2626
Fragments arranged by size:
4933 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4870 5044 5208 5454 669
Size: 988 281 2932 174 164 246 3079
Fragments arranged by size:
3079 2932 988 281 246 174 164
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7864
HpaI GTT'AAC
Cuts at: 5454 5454
Size: 7864
KasI G'GCGC_C
Cuts at: 4581 4581
Size: 7864
KpnI G_GTAC'C
Cuts at: 5189 5189
Size: 7864
MfeI C'AATT_G
Cuts at: 4040 5463 4040
Size: 1423 6441
MluI A'CGCG_T
Cuts at: 7740 7740
Size: 7864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6978 7162 1305
Size: 5673 184 2007
Fragments arranged by size:
5673 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4430 4910 10
Size: 54 1425 343 2598 480 2964
Fragments arranged by size:
2964 2598 1425 480 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2502 3365 3869 3884 4007 4280 4460
Size: 648 1336 863 504 15 123 273 180
Cuts at: 4460 5072 5822 6181 6340 518
Size: 612 750 359 159 2042
Fragments arranged by size:
2042 1336 863 750 648 612 504 359
273 180 159 123 15
MspA1I CmG'CkG
Cuts at: 1606 2293 2989 4072 4404 4719 4731 4737 5083
Size: 687 696 1083 332 315 12 6 346
Cuts at: 5083 5156 5280 5957 7105 7350 1606
Size: 73 124 677 1148 245 2120
Fragments arranged by size:
2120 1148 1083 696 687 677 346 332
315 245 124 73 12 6
NaeI GCC'GGC
Cuts at: 5094 5094
Size: 7864
NarI GG'CG_CC
Cuts at: 4582 4582
Size: 7864
NcoI C'CATG_G
Cuts at: 513 1065 2041 513
Size: 552 976 6336
Fragments arranged by size:
6336 976 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7864
NgoMIV G'CCGG_C
Cuts at: 5092 5092
Size: 7864
NheI G'CTAG_C
Cuts at: 1052 3619 1052
Size: 2567 5297
NotI GC'GGCC_GC
Cuts at: 5311 5311
Size: 7864
NspI r_CATG'y
Cuts at: 1132 1229 3844 4143 4279 5077 5136 5222 6767
Size: 97 2615 299 136 798 59 86 1545
Cuts at: 6767 1132
Size: 2229
Fragments arranged by size:
2615 2229 1545 798 299 136 97 86
59
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7864
PciI A'CATG_T
Cuts at: 3840 6763 3840
Size: 2923 4941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2071 3469 1108
Size: 963 1398 5503
Fragments arranged by size:
5503 1398 963
PfoI T'CCnGG_A
Cuts at: 2220 3447 4110 4482 2220
Size: 1227 663 372 5602
Fragments arranged by size:
5602 1227 663 372
PmeI GTTT'AAAC
Cuts at: 5168 5168
Size: 7864
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5933 5965 7446 7478 5933
Size: 32 1481 32 6319
Fragments arranged by size:
6319 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4087 4087
Size: 7864
PshAI GACnn'nnGTC
Cuts at: 1613 5030 1613
Size: 3417 4447
PsiI TTA'TAA
Cuts at: 2490 3816 5434 2490
Size: 1326 1618 4920
Fragments arranged by size:
4920 1618 1326
PspOMI G'GGCC_C
Cuts at: 3218 3218
Size: 7864
PspXI vC'TCGA_Gb
Cuts at: 3119 3119
Size: 7864
PstI C_TGCA'G
Cuts at: 830 2301 5216 830
Size: 1471 2915 3478
Fragments arranged by size:
3478 2915 1471
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7864
PvuII CAG'CTG
Cuts at: 2293 2293
Size: 7864
SalI G'TCGA_C
Cuts at: 1936 5206 1936
Size: 3270 4594
SapI GCTCTTCn'nnn_
Cuts at: 2870 2870
Size: 7864
SbfI CC_TGCA'GG
Cuts at: 5216 5216
Size: 7864
ScaI AGT'ACT
Cuts at: 6112 7824 6112
Size: 1712 6152
SfcI C'TryA_G
Cuts at: 826 1045 2297 5212 6347 7028 7219 826
Size: 219 1252 2915 1135 681 191 1471
Fragments arranged by size:
2915 1471 1252 1135 681 219 191
SfoI GGC'GCC
Cuts at: 4583 4583
Size: 7864
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7864
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7864
SmaI CCC'GGG
Cuts at: 1872 5191 1872
Size: 3319 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2081 3119 3131 5968 6869 7131
Size: 197 926 138 1038 12 2837 901 262
Cuts at: 7131 820
Size: 1553
Fragments arranged by size:
2837 1553 1038 926 901 262 197 138
12
SnaBI TAC'GTA
Cuts at: 493 2371 5655 493
Size: 1878 3284 2702
Fragments arranged by size:
3284 2702 1878
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7864
SphI G_CATG'C
Cuts at: 1132 5077 5222 1132
Size: 3945 145 3774
Fragments arranged by size:
3945 3774 145
SspI AAT'ATT
Cuts at: 5 52 5788 5
Size: 47 5736 2081
Fragments arranged by size:
5736 2081 47
StuI AGG'CCT
Cuts at: 1517 4263 1517
Size: 2746 5118
StyI C'CwwG_G
Cuts at: 513 1065 2041 2070 4155 5303 513
Size: 552 976 29 2085 1148 3074
Fragments arranged by size:
3074 2085 1148 976 552 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2017 5891 5908 6061 6246 6691 325
Size: 1255 437 3874 17 153 185 445 1498
Fragments arranged by size:
3874 1498 1255 445 437 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2517 2883 4142
Size: 274 80 33 51 1443 540 366 1259
Cuts at: 4142 6110 7822 96
Size: 1968 1712 138
Fragments arranged by size:
1968 1712 1443 1259 540 366 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7824 7856 7824
Size: 32 7832
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7864
XbaI T'CTAG_A
Cuts at: 5200 5200
Size: 7864
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4423 4513 4757 4903 4423
Size: 90 244 146 7384
Fragments arranged by size:
7384 244 146 90
XhoI C'TCGA_G
Cuts at: 1943 3119 1943
Size: 1176 6688
XmaI C'CCGG_G
Cuts at: 1870 5189 1870
Size: 3319 4545
XmnI GAAnn'nnTTC
Cuts at: 3012 3792 5993 3012
Size: 780 2201 4883
Fragments arranged by size:
4883 2201 780
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5672 276
Size: 53 83 186 936 4138 2468
Fragments arranged by size:
4138 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PsrI RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI