Restriction map for
pFN21AA0336
(Circular) (Six-base) MAPSORT of: pFN21AA0336.seq Check: 2478 from: 1 to: 9454
pFN21AA0336 9454 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7264 278
Size: 53 83 186 936 5728 2468
Fragments arranged by size:
5728 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3411 4998 6797 1937
Size: 1474 1587 1799 4594
Fragments arranged by size:
4594 1799 1587 1474
Acc65I G'GTAC_C
Cuts at: 3361 6775 3361
Size: 3414 6040
AclI AA'CG_TT
Cuts at: 1563 5200 7581 7954 1563
Size: 3637 2381 373 3063
Fragments arranged by size:
3637 3063 2381 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2250 3753 3777 3878 4518 5009 5066 5099
Size: 618 1503 24 101 640 491 57 33
Cuts at: 5099 5313 6672 7517 8901 9169 1632
Size: 214 1359 845 1384 268 1917
Fragments arranged by size:
1917 1503 1384 1359 845 640 618 491
268 214 101 57 33 24
AflII C'TTAA_G
Cuts at: 820 1017 2783 2987 3086 4187 4228 6125 820
Size: 197 1766 204 99 1101 41 1897 4149
Fragments arranged by size:
4149 1897 1766 1101 204 197 99 41
AflIII A'CryG_T
Cuts at: 1203 4364 8353 9330 1203
Size: 3161 3989 977 1327
Fragments arranged by size:
3989 3161 1327 977
AgeI A'CCGG_T
Cuts at: 8285 8285
Size: 9454
AhdI GACnn_n'nnGTC
Cuts at: 5988 8183 5988
Size: 2195 7259
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9454
AlwNI CAG_nnn'CTG
Cuts at: 5420 7197 8769 5420
Size: 1777 1572 6105
Fragments arranged by size:
6105 1777 1572
ApaLI G'TGCA_C
Cuts at: 7511 8667 7511
Size: 1156 8298
ApoI r'AATT_y
Cuts at: 1493 2098 2213 2292 2602 3165 3469 3514 4867
Size: 605 115 79 310 563 304 45 1353
Cuts at: 4867 4894 5274 5657 6365 6763 6989 7141 9250
Size: 27 380 383 708 398 226 152 2109
Cuts at: 9250 1493
Size: 1697
Fragments arranged by size:
2109 1697 1353 708 605 563 398 383
380 310 304 226 152 115 79 45
27
AseI AT'TA_AT
Cuts at: 160 1032 2403 2862 3065 5823 8008 160
Size: 872 1371 459 203 2758 2185 1606
Fragments arranged by size:
2758 2185 1606 1371 872 459 203
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9454
AvaI C'yCGr_G
Cuts at: 1870 1943 6234 6779 1870
Size: 73 4291 545 4545
Fragments arranged by size:
4545 4291 545 73
BamHI G'GATC_C
Cuts at: 6784 7151 6784
Size: 367 9087
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3361 6775 8230 618
Size: 325 217 627 1574 3414 1455 1842
Fragments arranged by size:
3414 1842 1574 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 6773 1391
Size: 5382 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2148 2850 3741 4023 4524 4851 5319
Size: 971 249 702 891 282 501 327 468
Cuts at: 5319 6273 928
Size: 954 4109
Fragments arranged by size:
4109 971 954 891 702 501 468 327
282 249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7645 7679 1903
Size: 34 5708 34 3678
Fragments arranged by size:
5708 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7346 8556 7346
Size: 1210 8244
BclI T'GATC_A
Cuts at: 1548 5422 1548
Size: 3874 5580
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4628 8065 136
Size: 107 122 71 631 605 2956 3437 1525
Fragments arranged by size:
3437 2956 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4732 4909 9449 4732
Size: 177 4540 4737
Fragments arranged by size:
4737 4540 177
BlpI GC'TnA_GC
Cuts at: 6644 6871 6644
Size: 227 9227
Bme1580I G_kGCm'C
Cuts at: 1790 7515 8671 1790
Size: 5725 1156 2573
Fragments arranged by size:
5725 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3191 6146 7185
Size: 416 53 83 399 24 1774 2955 1039
Cuts at: 7185 8143 442
Size: 958 1753
Fragments arranged by size:
2955 1774 1753 1039 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2927 1056
Size: 1871 7583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3305 5480 8114 1239
Size: 2066 2175 2634 2579
Fragments arranged by size:
2634 2579 2175 2066
Bpu10I CC'TnA_GC
Cuts at: 2490 4495 2490
Size: 2005 7449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6362 6666 7579 8444 8742 6362
Size: 304 913 865 298 7074
Fragments arranged by size:
7074 913 865 304 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4655 8117 882
Size: 617 3156 3462 2219
Fragments arranged by size:
3462 3156 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 7245 493
Size: 711 6041 2702
Fragments arranged by size:
6041 2702 711
BsaBI GATnn'nnATC
Cuts at: 6721 9448 6721
Size: 2727 6727
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7261 7643 8278 275
Size: 53 83 186 936 5728 382 635 1451
Fragments arranged by size:
5728 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7887 8285 8559 8706 9117 7887
Size: 398 274 147 411 8224
Fragments arranged by size:
8224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6263 6293 1178
Size: 30 165 30 4860 30 4339
Fragments arranged by size:
4860 4339 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 3674 4607 1186
Size: 1114 1374 933 6033
Fragments arranged by size:
6033 1374 1114 933
BseYI C'CCAG_C
Cuts at: 8657 9153 8657
Size: 496 8958
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 6435 1163
Size: 573 4699 4182
Fragments arranged by size:
4699 4182 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6904 7665 8693 1540
Size: 275 186 4903 761 1028 2301
Fragments arranged by size:
4903 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5541 7515 7600 8671 5541
Size: 1974 85 1071 6324
Fragments arranged by size:
6324 1974 1071 85
BsiWI C'GTAC_G
Cuts at: 5570 5570
Size: 9454
BsmI GAATG_Cn'
Cuts at: 4303 4583 6965 7058 4303
Size: 280 2382 93 6699
Fragments arranged by size:
6699 2382 280 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 5541 6773 7515 7600 8671 1391
Size: 399 3751 1232 742 85 1071 2174
Fragments arranged by size:
3751 2174 1232 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 9117 9117
Size: 9454
BspHI T'CATG_A
Cuts at: 2833 3250 7343 9073 2833
Size: 417 4093 1730 3214
Fragments arranged by size:
4093 3214 1730 417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2918 6809 844
Size: 2074 3891 3489
Fragments arranged by size:
3891 3489 2074
BsrBI CCG'CTC
Cuts at: 1394 1823 7341 1394
Size: 429 5518 3507
Fragments arranged by size:
5518 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2184 4998 5843 7949 8123 65
Size: 1183 936 2814 845 2106 174 1396
Fragments arranged by size:
2814 2106 1396 1183 936 845 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8098 8285 1700
Size: 110 6288 187 2869
Fragments arranged by size:
6288 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9454
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9454
BssSI C'ACGA_G
Cuts at: 5534 7514 8526 5534
Size: 1980 1012 6462
Fragments arranged by size:
6462 1980 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6458 794
Size: 5664 3790
BstBI TT'CG_AA
Cuts at: 3216 6019 8258 3216
Size: 2803 2239 4412
Fragments arranged by size:
4412 2803 2239
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9454
BstXI CCAn_nnnn'nTGG
Cuts at: 4993 4993
Size: 9454
BstYI r'GATC_y
Cuts at: 1972 4732 4909 6784 7151 7536 7553 9005 9091
Size: 2760 177 1875 367 385 17 1452 86
Cuts at: 9091 9103 9449 1972
Size: 12 346 1977
Fragments arranged by size:
2760 1977 1875 1452 385 367 346 177
86 17 12
BstZ17I GTA'TAC
Cuts at: 3412 3412
Size: 9454
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 8902
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8715
Fragments arranged by size:
8715 683 56
BtsI GCAGTG_nn'
Cuts at: 4886 5864 6970 7764 7784 4886
Size: 978 1106 794 20 6556
Fragments arranged by size:
6556 1106 978 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9454
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9419
DraI TTT'AAA
Cuts at: 2857 2934 3135 3669 3924 5448 6758 2857
Size: 77 201 534 255 1524 1310 5553
Fragments arranged by size:
5553 1524 1310 534 255 201 77
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3034 4936 8461 809
Size: 507 1718 1902 3525 1802
Fragments arranged by size:
3525 1902 1802 1718 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6901 7790 9419
Size: 54 1425 50 275 18 5071 889 1629
Cuts at: 9419 8
Size: 43
Fragments arranged by size:
5071 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6901 1537
Size: 275 5089 4090
Fragments arranged by size:
5089 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 2412 2445 3903 4020 4155 4698 5607
Size: 897 162 33 1458 117 135 543 909
Cuts at: 5607 6122 6234 7384 1353
Size: 515 112 1150 3423
Fragments arranged by size:
3423 1458 1150 909 897 543 515 162
135 117 112 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5930 6274 8041 8415 8561 387
Size: 1064 138 4341 344 1767 374 146 1280
Fragments arranged by size:
4341 1767 1280 1064 374 344 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 3305 3753 3777 3878 4518 5009
Size: 393 618 1055 448 24 101 640 491
Cuts at: 5009 5066 5099 5313 5480 6672 7517 8114 8901
Size: 57 33 214 167 1192 845 597 787
Cuts at: 8901 9169 1239
Size: 268 1524
Fragments arranged by size:
1524 1192 1055 845 787 640 618 597
491 448 393 268 214 167 101 57
33 24
EcoO109I rG'GnC_Cy
Cuts at: 4267 6013 4267
Size: 1746 7708
EcoRI G'AATT_C
Cuts at: 5657 6763 7141 5657
Size: 1106 378 7970
Fragments arranged by size:
7970 1106 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3314 4510 6583 3314
Size: 1196 2073 6185
Fragments arranged by size:
6185 2073 1196
FspI TGC'GCA
Cuts at: 7960 7960
Size: 9454
HaeII r_GCGC'y
Cuts at: 1678 5742 8601 1678
Size: 4064 2859 2531
Fragments arranged by size:
4064 2859 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3203 3235 3457 3489 4514
Size: 32 135 32 1631 32 222 32 1025
Cuts at: 4514 4546 4700 4732 5974 6006 6257 6263 6289
Size: 32 154 32 1242 32 251 6 26
Cuts at: 6289 6295 8095 8127 8169 8201 1373
Size: 6 1800 32 42 32 2626
Fragments arranged by size:
2626 1800 1631 1242 1025 251 222 154
135 42 32 32 32 32 32 32
32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 5229 6798 7044 669
Size: 988 281 3291 1569 246 3079
Fragments arranged by size:
3291 3079 1569 988 281 246
HindIII A'AGCT_T
Cuts at: 748 6482 748
Size: 5734 3720
HpaI GTT'AAC
Cuts at: 5229 7044 5229
Size: 1815 7639
KpnI G_GTAC'C
Cuts at: 3365 6779 3365
Size: 3414 6040
MfeI C'AATT_G
Cuts at: 5369 7053 5369
Size: 1684 7770
MluI A'CGCG_T
Cuts at: 9330 9330
Size: 9454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2040 4527 8568 8752 1305
Size: 735 2487 4041 184 2007
Fragments arranged by size:
4041 2487 2007 735 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7632
Fragments arranged by size:
7632 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5171 5960 7412 7771 7930 518
Size: 648 4005 789 1452 359 159 2042
Fragments arranged by size:
4005 2042 1452 789 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2167 3755 5500 6316 6870 7547 8695 8940
Size: 561 1588 1745 816 554 677 1148 245
Cuts at: 8940 1606
Size: 2120
Fragments arranged by size:
2120 1745 1588 1148 816 677 561 554
245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8902
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9454
NheI G'CTAG_C
Cuts at: 1052 2923 1052
Size: 1871 7583
NotI GC'GGCC_GC
Cuts at: 6901 6901
Size: 9454
NspI r_CATG'y
Cuts at: 1132 1229 4368 5054 6223 6362 6812 8357 1132
Size: 97 3139 686 1169 139 450 1545 2229
Fragments arranged by size:
3139 2229 1545 1169 686 450 139 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9454
PciI A'CATG_T
Cuts at: 4364 8353 4364
Size: 3989 5465
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9454
PfoI T'CCnGG_A
Cuts at: 5922 5922
Size: 9454
PmeI GTTT'AAAC
Cuts at: 6758 6758
Size: 9454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7523 7555 9036 9068 7523
Size: 32 1481 32 7909
Fragments arranged by size:
7909 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 6013 6013
Size: 9454
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9454
PsiI TTA'TAA
Cuts at: 3500 4409 6626 7024 3500
Size: 909 2217 398 5930
Fragments arranged by size:
5930 2217 909 398
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3265 3297 5070 5102 3265
Size: 32 1773 32 7617
Fragments arranged by size:
7617 1773 32 32
PstI C_TGCA'G
Cuts at: 830 3261 4275 5895 6541 6576 6806 830
Size: 2431 1014 1620 646 35 230 3478
Fragments arranged by size:
3478 2431 1620 1014 646 230 35
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9454
PvuII CAG'CTG
Cuts at: 2167 3755 5500 6316 2167
Size: 1588 1745 816 5305
Fragments arranged by size:
5305 1745 1588 816
SalI G'TCGA_C
Cuts at: 1936 6796 1936
Size: 4860 4594
SapI GCTCTTCn'nnn_
Cuts at: 2412 3903 6122 2412
Size: 1491 2219 5744
Fragments arranged by size:
5744 2219 1491
SbfI CC_TGCA'GG
Cuts at: 6806 6806
Size: 9454
ScaI AGT'ACT
Cuts at: 7702 9414 7702
Size: 1712 7742
SexAI A'CCwGG_T
Cuts at: 6592 6592
Size: 9454
SfcI C'TryA_G
Cuts at: 826 1045 2955 3107 3257 4049 4271 5606 5891
Size: 219 1910 152 150 792 222 1335 285
Cuts at: 5891 6537 6572 6802 7937 8618 8809 826
Size: 646 35 230 1135 681 191 1471
Fragments arranged by size:
1910 1471 1335 1135 792 681 646 285
230 222 219 191 152 150 35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9454
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9454
SmaI CCC'GGG
Cuts at: 1872 6236 6781 1872
Size: 4364 545 4545
Fragments arranged by size:
4545 4364 545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2783 2987 3086 4187 4228 6125
Size: 197 926 840 204 99 1101 41 1897
Cuts at: 6125 6341 6681 7558 8459 8721 820
Size: 216 340 877 901 262 1553
Fragments arranged by size:
1897 1553 1101 926 901 877 840 340
262 216 204 197 99 41
SnaBI TAC'GTA
Cuts at: 493 7245 493
Size: 6752 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9454
SphI G_CATG'C
Cuts at: 1132 6362 6812 1132
Size: 5230 450 3774
Fragments arranged by size:
5230 3774 450
SspI AAT'ATT
Cuts at: 5 52 2401 7378 5
Size: 47 2349 4977 2081
Fragments arranged by size:
4977 2349 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9454
StyI C'CwwG_G
Cuts at: 513 1065 4068 5628 6893 513
Size: 552 3003 1560 1265 3074
Fragments arranged by size:
3074 3003 1560 1265 552
SwaI ATTT'AAAT
Cuts at: 3924 3924
Size: 9454
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7481 7498 7651 7836 8281 325
Size: 1255 5901 17 153 185 445 1498
Fragments arranged by size:
5901 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2977 3609 4960
Size: 274 80 33 51 1443 1000 632 1351
Cuts at: 4960 5332 7700 9412 96
Size: 372 2368 1712 138
Fragments arranged by size:
2368 1712 1443 1351 1000 632 372 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5918 5950 6102 6134 9414 9446 5918
Size: 32 152 32 3280 32 5926
Fragments arranged by size:
5926 3280 152 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9454
XbaI T'CTAG_A
Cuts at: 4285 6214 6790 4285
Size: 1929 576 6949
Fragments arranged by size:
6949 1929 576
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5392 6379 5392
Size: 987 8467
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9454
XmaI C'CCGG_G
Cuts at: 1870 6234 6779 1870
Size: 4364 545 4545
Fragments arranged by size:
4545 4364 545
XmnI GAAnn'nnTTC
Cuts at: 4692 5022 5079 6384 7583 4692
Size: 330 57 1305 1199 6563
Fragments arranged by size:
6563 1305 1199 330 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7262 276
Size: 53 83 186 936 5728 2468
Fragments arranged by size:
5728 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsmBI Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII
SanDI SfiI SfoI SrfI