Restriction map for
pFN21AA0335
(Circular) (Six-base) MAPSORT of: pFN21AA0335.seq Check: 2168 from: 1 to: 9154
pFN21AA0335 9154 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6964 278
Size: 53 83 186 936 5428 2468
Fragments arranged by size:
5428 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6497 1937
Size: 4560 4594
Acc65I G'GTAC_C
Cuts at: 3779 6475 3779
Size: 2696 6458
AclI AA'CG_TT
Cuts at: 1563 7281 7654 1563
Size: 5718 373 3063
Fragments arranged by size:
5718 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2340 2735 3099 3237 4349 4368 5433 5940
Size: 708 395 364 138 1112 19 1065 507
Cuts at: 5940 7217 8601 8869 1632
Size: 1277 1384 268 1917
Fragments arranged by size:
1917 1384 1277 1112 1065 708 507 395
364 268 138 19
AflII C'TTAA_G
Cuts at: 820 1017 2936 3047 4667 5357 6128 820
Size: 197 1919 111 1620 690 771 3846
Fragments arranged by size:
3846 1919 1620 771 690 197 111
AflIII A'CryG_T
Cuts at: 1203 2842 6121 6444 8053 9030 1203
Size: 1639 3279 323 1609 977 1327
Fragments arranged by size:
3279 1639 1609 1327 977 323
AgeI A'CCGG_T
Cuts at: 7985 7985
Size: 9154
AhdI GACnn_n'nnGTC
Cuts at: 7883 7883
Size: 9154
AleI CACnn'nnGTG
Cuts at: 1166 3041 1166
Size: 1875 7279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9154
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3565 3597 3565
Size: 32 9122
AlwNI CAG_nnn'CTG
Cuts at: 3431 5682 5832 6897 8469 3431
Size: 2251 150 1065 1572 4116
Fragments arranged by size:
4116 2251 1572 1065 150
ApaLI G'TGCA_C
Cuts at: 7211 8367 7211
Size: 1156 7998
ApoI r'AATT_y
Cuts at: 1493 2112 2697 3697 3710 4222 4334 4570 4577
Size: 619 585 1000 13 512 112 236 7
Cuts at: 4577 4644 4735 4771 5442 5763 6110 6347 6463
Size: 67 91 36 671 321 347 237 116
Cuts at: 6463 6689 6841 8950 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1000 671 619 585 512 347
321 237 236 226 152 116 112 91
67 36 13 7
AseI AT'TA_AT
Cuts at: 160 1032 3864 4548 7708 160
Size: 872 2832 684 3160 1606
Fragments arranged by size:
3160 2832 1606 872 684
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9154
AvaI C'yCGr_G
Cuts at: 1870 1943 3767 6432 6479 1870
Size: 73 1824 2665 47 4545
Fragments arranged by size:
4545 2665 1824 73 47
AvrII C'CTAG_G
Cuts at: 5954 6026 5954
Size: 72 9082
BamHI G'GATC_C
Cuts at: 6484 6851 6484
Size: 367 8787
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 2430 2813 3385 3779
Size: 325 217 627 311 332 383 572 394
Cuts at: 3779 4994 6475 7930 618
Size: 1215 1481 1455 1842
Fragments arranged by size:
1842 1481 1455 1215 627 572 394 383
332 325 311 217
BanII G_rGCy'C
Cuts at: 1391 4076 6473 1391
Size: 2685 2397 4072
Fragments arranged by size:
4072 2685 2397
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2984 3087 3257 4263 6323 928
Size: 971 1085 103 170 1006 2060 3759
Fragments arranged by size:
3759 2060 1085 1006 971 170 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 7345 7379 1903
Size: 34 50 34 5324 34 3678
Fragments arranged by size:
5324 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4910 6382 7046 8256 4910
Size: 1472 664 1210 5808
Fragments arranged by size:
5808 1472 1210 664
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9154
BfrBI ATG'CAT
Cuts at: 3830 3850 3830
Size: 20 9134
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7765 136
Size: 107 122 71 631 605 6093 1525
Fragments arranged by size:
6093 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9149 9149
Size: 9154
BlpI GC'TnA_GC
Cuts at: 6571 6571
Size: 9154
Bme1580I G_kGCm'C
Cuts at: 1790 4999 7215 8371 1790
Size: 3209 2216 1156 2573
Fragments arranged by size:
3209 2573 2216 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3207 4517 6885
Size: 416 53 83 399 24 1790 1310 2368
Cuts at: 6885 7843 442
Size: 958 1753
Fragments arranged by size:
2368 1790 1753 1310 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4298 7814 1239
Size: 3059 3516 2579
Fragments arranged by size:
3516 3059 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3024 7279 8144 8442 3024
Size: 4255 865 298 3736
Fragments arranged by size:
4255 3736 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7817 882
Size: 617 6318 2219
Fragments arranged by size:
6318 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6945 493
Size: 711 5741 2702
Fragments arranged by size:
5741 2702 711
BsaBI GATnn'nnATC
Cuts at: 9148 9148
Size: 9154
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6961 7343 7978 275
Size: 53 83 186 936 5428 382 635 1451
Fragments arranged by size:
5428 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7587 7985 8259 8406 8817 7587
Size: 398 274 147 411 7924
Fragments arranged by size:
7924 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8929
Fragments arranged by size:
8929 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 3177 5562 1186
Size: 1114 877 2385 4778
Fragments arranged by size:
4778 2385 1114 877
BseYI C'CCAG_C
Cuts at: 4999 8357 8853 4999
Size: 3358 496 5300
Fragments arranged by size:
5300 3358 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8581
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6604 7365 8393 1540
Size: 275 186 4603 761 1028 2301
Fragments arranged by size:
4603 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4076 5225 7215 7300 8371 4076
Size: 1149 1990 85 1071 4859
Fragments arranged by size:
4859 1990 1149 1071 85
BsmI GAATG_Cn'
Cuts at: 6665 6758 6665
Size: 93 9061
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4076 4999 5225 6473 7215 7300 8371
Size: 399 2286 923 226 1248 742 85 1071
Cuts at: 8371 1391
Size: 2174
Fragments arranged by size:
2286 2174 1248 1071 923 742 399 226
85
BspEI T'CCGG_A
Cuts at: 8817 8817
Size: 9154
BspHI T'CATG_A
Cuts at: 3246 7043 8773 3246
Size: 3797 1730 3627
Fragments arranged by size:
3797 3627 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6509 844
Size: 5665 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 7041 1394
Size: 429 5218 3507
Fragments arranged by size:
5218 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2294 5507 5885 7649 7823 65
Size: 1183 1046 3213 378 1764 174 1396
Fragments arranged by size:
3213 1764 1396 1183 1046 378 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7798 7985 1700
Size: 110 5988 187 2869
Fragments arranged by size:
5988 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2291 4203 96
Size: 2195 1912 5047
Fragments arranged by size:
5047 2195 1912
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9154
BssSI C'ACGA_G
Cuts at: 4463 5004 7214 8226 4463
Size: 541 2210 1012 5391
Fragments arranged by size:
5391 2210 1012 541
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9154
BstBI TT'CG_AA
Cuts at: 4493 7958 4493
Size: 3465 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9154
BstXI CCAn_nnnn'nTGG
Cuts at: 2660 2660
Size: 9154
BstYI r'GATC_y
Cuts at: 1972 6484 6851 7236 7253 8705 8791 8803 9149
Size: 4512 367 385 17 1452 86 12 346
Cuts at: 9149 1972
Size: 1977
Fragments arranged by size:
4512 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2434 513
Size: 552 1369 7233
Fragments arranged by size:
7233 1369 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8415
Fragments arranged by size:
8415 683 56
BtsI GCAGTG_nn'
Cuts at: 3984 4543 6670 7464 7484 3984
Size: 559 2127 794 20 5654
Fragments arranged by size:
5654 2127 794 559 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3694 3729 529
Size: 35 3130 35 5954
Fragments arranged by size:
5954 3130 35 35
DraI TTT'AAA
Cuts at: 2055 2370 2500 4113 4134 4770 5325 5460 6235
Size: 315 130 1613 21 636 555 135 775
Cuts at: 6235 6458 2055
Size: 223 4751
Fragments arranged by size:
4751 1613 775 636 555 315 223 135
130 21
DraIII CAC_nnn'GTG
Cuts at: 2609 2609
Size: 9154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8161 809
Size: 507 6845 1802
Fragments arranged by size:
6845 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6601 7490 9119
Size: 54 1425 50 275 18 4771 889 1629
Cuts at: 9119 8
Size: 43
Fragments arranged by size:
4771 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6601 1537
Size: 275 4789 4090
Fragments arranged by size:
4789 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 3213 7084 1353
Size: 966 894 3871 3423
Fragments arranged by size:
3871 3423 966 894
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7741 8115 8261 387
Size: 1064 138 6152 374 146 1280
Fragments arranged by size:
6152 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4074 4074
Size: 9154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2340 2735 3099 3237 4298 4349 4368
Size: 393 708 395 364 138 1061 51 19
Cuts at: 4368 5433 5940 7217 7814 8601 8869 1239
Size: 1065 507 1277 597 787 268 1524
Fragments arranged by size:
1524 1277 1065 1061 787 708 597 507
395 393 364 268 138 51 19
EcoO109I rG'GnC_Cy
Cuts at: 3266 5150 3266
Size: 1884 7270
EcoRI G'AATT_C
Cuts at: 6463 6841 6463
Size: 378 8776
EcoRV GAT'ATC
Cuts at: 3606 3606
Size: 9154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2765 3672 4020 5207 2765
Size: 907 348 1187 6712
Fragments arranged by size:
6712 1187 907 348
FspI TGC'GCA
Cuts at: 7660 7660
Size: 9154
HaeII r_GCGC'y
Cuts at: 1678 8301 1678
Size: 6623 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5552 5584 7795 7827 7869
Size: 32 135 32 3980 32 2211 32 42
Cuts at: 7869 7901 1373
Size: 32 2626
Fragments arranged by size:
3980 2626 2211 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 3733 3808 6498 6744 669
Size: 988 281 268 1527 75 2690 246 3079
Fragments arranged by size:
3079 2690 1527 988 281 268 246 75
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9154
HpaI GTT'AAC
Cuts at: 6744 6744
Size: 9154
KpnI G_GTAC'C
Cuts at: 3783 6479 3783
Size: 2696 6458
MfeI C'AATT_G
Cuts at: 4358 6753 4358
Size: 2395 6759
MluI A'CGCG_T
Cuts at: 9030 9030
Size: 9154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3924 4051 8268 8452 1305
Size: 2619 127 4217 184 2007
Fragments arranged by size:
4217 2619 2007 184 127
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7332
Fragments arranged by size:
7332 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2871 3041 3111 3547 7112 7471 7630
Size: 648 1705 170 70 436 3565 359 159
Cuts at: 7630 518
Size: 2042
Fragments arranged by size:
3565 2042 1705 648 436 359 170 159
70
MspA1I CmG'CkG
Cuts at: 1606 2364 4979 6570 7247 8395 8640 1606
Size: 758 2615 1591 677 1148 245 2120
Fragments arranged by size:
2615 2120 1591 1148 758 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2434 513
Size: 552 1369 7233
Fragments arranged by size:
7233 1369 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9154
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9154
NotI GC'GGCC_GC
Cuts at: 6601 6601
Size: 9154
NsiI A_TGCA'T
Cuts at: 3832 3852 3832
Size: 20 9134
NspI r_CATG'y
Cuts at: 1132 1229 2846 5206 5646 6125 6448 6512 8057
Size: 97 1617 2360 440 479 323 64 1545
Cuts at: 8057 1132
Size: 2229
Fragments arranged by size:
2360 2229 1617 1545 479 440 323 97
64
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9154
PciI A'CATG_T
Cuts at: 2842 6121 6444 8053 2842
Size: 3279 323 1609 3943
Fragments arranged by size:
3943 3279 1609 323
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3469 5006 1108
Size: 2361 1537 5256
Fragments arranged by size:
5256 2361 1537
PfoI T'CCnGG_A
Cuts at: 4864 4864
Size: 9154
PmeI GTTT'AAAC
Cuts at: 6458 6458
Size: 9154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7223 7255 8736 8768 7223
Size: 32 1481 32 7609
Fragments arranged by size:
7609 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3266 3266
Size: 9154
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9154
PsiI TTA'TAA
Cuts at: 2706 2849 6183 6724 2706
Size: 143 3334 541 5136
Fragments arranged by size:
5136 3334 541 143
PstI C_TGCA'G
Cuts at: 830 2932 6506 830
Size: 2102 3574 3478
Fragments arranged by size:
3574 3478 2102
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9154
PvuII CAG'CTG
Cuts at: 2364 2364
Size: 9154
SacI G_AGCT'C
Cuts at: 4076 4076
Size: 9154
SalI G'TCGA_C
Cuts at: 1936 6496 1936
Size: 4560 4594
SbfI CC_TGCA'GG
Cuts at: 6506 6506
Size: 9154
ScaI AGT'ACT
Cuts at: 5383 7402 9114 5383
Size: 2019 1712 5423
Fragments arranged by size:
5423 2019 1712
SfcI C'TryA_G
Cuts at: 826 1045 2928 6502 7637 8318 8509 826
Size: 219 1883 3574 1135 681 191 1471
Fragments arranged by size:
3574 1883 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9154
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9154
SmaI CCC'GGG
Cuts at: 1872 6481 1872
Size: 4609 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2936 3039 3047 4667 5357 6128
Size: 197 926 993 103 8 1620 690 771
Cuts at: 6128 7258 8159 8421 820
Size: 1130 901 262 1553
Fragments arranged by size:
1620 1553 1130 993 926 901 771 690
262 197 103 8
SnaBI TAC'GTA
Cuts at: 493 6945 493
Size: 6452 2702
SpeI A'CTAG_T
Cuts at: 152 4057 152
Size: 3905 5249
SphI G_CATG'C
Cuts at: 1132 6512 1132
Size: 5380 3774
SspI AAT'ATT
Cuts at: 5 52 2173 2801 5433 6334 7078 5
Size: 47 2121 628 2632 901 744 2081
Fragments arranged by size:
2632 2121 2081 901 744 628 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9154
StyI C'CwwG_G
Cuts at: 513 1065 2434 3904 5954 6026 6593 513
Size: 552 1369 1470 2050 72 567 3074
Fragments arranged by size:
3074 2050 1470 1369 567 552 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2666 7181 7198 7351 7536 7981 325
Size: 1255 1086 4515 17 153 185 445 1498
Fragments arranged by size:
4515 1498 1255 1086 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2291 3259 4203
Size: 274 80 33 51 1443 314 968 944
Cuts at: 4203 5381 6124 7400 9112 96
Size: 1178 743 1276 1712 138
Fragments arranged by size:
1712 1443 1276 1178 968 944 743 314
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3963 3995 4884 4916 9114 9146 3963
Size: 32 889 32 4198 32 3971
Fragments arranged by size:
4198 3971 889 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4675 1316
Size: 3359 5795
XbaI T'CTAG_A
Cuts at: 5570 5819 6490 5570
Size: 249 671 8234
Fragments arranged by size:
8234 671 249
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9154
XmaI C'CCGG_G
Cuts at: 1870 6479 1870
Size: 4609 4545
XmnI GAAnn'nnTTC
Cuts at: 2981 4499 7283 2981
Size: 1518 2784 4852
Fragments arranged by size:
4852 2784 1518
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6962 276
Size: 53 83 186 936 5428 2468
Fragments arranged by size:
5428 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI