Restriction map for
pFN21AA0321
(Circular) (Six-base) MAPSORT of: pFN21AA0321.seq Check: 480 from: 1 to: 12322
pFN21AA0321 12322 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6550 9502 6550
Size: 2952 9370
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10132 278
Size: 53 83 186 936 8596 2468
Fragments arranged by size:
8596 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5026 7336 9665 1937
Size: 3089 2310 2329 4594
Fragments arranged by size:
4594 3089 2329 2310
Acc65I G'GTAC_C
Cuts at: 7432 9643 7432
Size: 2211 10111
AclI AA'CG_TT
Cuts at: 1563 10449 10822 1563
Size: 8886 373 3063
Fragments arranged by size:
8886 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2150 4598 5069 5183 5261 5455 6278 6324
Size: 518 2448 471 114 78 194 823 46
Cuts at: 6324 7626 8294 8475 8812 8921 8941 8990 10385
Size: 1302 668 181 337 109 20 49 1395
Cuts at: 10385 11769 12037 1632
Size: 1384 268 1917
Fragments arranged by size:
2448 1917 1395 1384 1302 823 668 518
471 337 268 194 181 114 109 78
49 46 20
AfeI AGC'GCT
Cuts at: 4619 4747 4619
Size: 128 12194
AflII C'TTAA_G
Cuts at: 820 1017 5927 8882 820
Size: 197 4910 2955 4260
Fragments arranged by size:
4910 4260 2955 197
AflIII A'CryG_T
Cuts at: 1203 4286 11221 12198 1203
Size: 3083 6935 977 1327
Fragments arranged by size:
6935 3083 1327 977
AgeI A'CCGG_T
Cuts at: 11153 11153
Size: 12322
AhdI GACnn_n'nnGTC
Cuts at: 2445 5127 11051 2445
Size: 2682 5924 3716
Fragments arranged by size:
5924 3716 2682
AleI CACnn'nnGTG
Cuts at: 1166 6305 1166
Size: 5139 7183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6053 8118 9152 1184
Size: 4869 2065 1034 4354
Fragments arranged by size:
4869 4354 2065 1034
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4592 4624 4592
Size: 32 12290
AlwNI CAG_nnn'CTG
Cuts at: 2515 3019 3172 3461 3487 3647 3691 4561 5284
Size: 504 153 289 26 160 44 870 723
Cuts at: 5284 5478 5694 6163 6961 7114 7117 7229 7539
Size: 194 216 469 798 153 3 112 310
Cuts at: 7539 7951 8268 9277 9379 10065 11637 2515
Size: 412 317 1009 102 686 1572 3200
Fragments arranged by size:
3200 1572 1009 870 798 723 686 504
469 412 317 310 289 216 194 160
153 153 112 102 44 26 3
ApaI G_GGCC'C
Cuts at: 2624 3191 6292 2624
Size: 567 3101 8654
Fragments arranged by size:
8654 3101 567
ApaLI G'TGCA_C
Cuts at: 10379 11535 10379
Size: 1156 11166
ApoI r'AATT_y
Cuts at: 1493 3086 4049 7391 7778 9363 9631 9857 10009
Size: 1593 963 3342 387 1585 268 226 152
Cuts at: 10009 12118 1493
Size: 2109 1697
Fragments arranged by size:
3342 2109 1697 1593 1585 963 387 268
226 152
AseI AT'TA_AT
Cuts at: 160 1032 10876 160
Size: 872 9844 1606
Fragments arranged by size:
9844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12322
AvaI C'yCGr_G
Cuts at: 1870 1943 2268 2363 2881 3008 5830 5977 6063
Size: 73 325 95 518 127 2822 147 86
Cuts at: 6063 6285 7271 9591 9647 1870
Size: 222 986 2320 56 4545
Fragments arranged by size:
4545 2822 2320 986 518 325 222 147
127 95 86 73 56
BamHI G'GATC_C
Cuts at: 6971 9652 10019 6971
Size: 2681 367 9274
Fragments arranged by size:
9274 2681 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2632 5462 6037 7422 7432
Size: 325 217 627 845 2830 575 1385 10
Cuts at: 7432 9596 9643 11098 618
Size: 2164 47 1455 1842
Fragments arranged by size:
2830 2164 1842 1455 1385 845 627 575
325 217 47 10
BanII G_rGCy'C
Cuts at: 1391 2526 2624 2668 3191 3207 3238 3833 3863
Size: 1135 98 44 523 16 31 595 30
Cuts at: 3863 4872 5313 5519 5579 5706 5828 6132 6292
Size: 1009 441 206 60 127 122 304 160
Cuts at: 6292 9641 1391
Size: 3349 4072
Fragments arranged by size:
4072 3349 1135 1009 595 523 441 304
206 160 127 122 98 60 44 31
30 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2163 2337 3057 4905 5322 928
Size: 971 264 174 720 1848 417 7928
Fragments arranged by size:
7928 1848 971 720 417 264 174
BbvCI CC'TCA_GC
Cuts at: 2115 3604 3804 3864 2115
Size: 1489 200 60 10573
Fragments arranged by size:
10573 1489 200 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8372 8406 10513 10547 1903
Size: 34 6435 34 2107 34 3678
Fragments arranged by size:
6435 3678 2107 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8377 10214 11424 8377
Size: 1837 1210 9275
Fragments arranged by size:
9275 1837 1210
BclI T'GATC_A
Cuts at: 1548 5527 7597 1548
Size: 3979 2070 6273
Fragments arranged by size:
6273 3979 2070
BfrBI ATG'CAT
Cuts at: 6416 6416
Size: 12322
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3988 5645 7091
Size: 107 122 71 631 605 2316 1657 1446
Cuts at: 7091 7518 10933 136
Size: 427 3415 1525
Fragments arranged by size:
3415 2316 1657 1525 1446 631 605 427
122 107 71
BglII A'GATC_T
Cuts at: 3295 8088 8904 12317 3295
Size: 4793 816 3413 3300
Fragments arranged by size:
4793 3413 3300 816
BlpI GC'TnA_GC
Cuts at: 2783 4388 9739 2783
Size: 1605 5351 5366
Fragments arranged by size:
5366 5351 1605
Bme1580I G_kGCm'C
Cuts at: 1790 2624 2637 3191 5465 6292 7401 9568 9601
Size: 834 13 554 2274 827 1109 2167 33
Cuts at: 9601 10383 11539 1790
Size: 782 1156 2573
Fragments arranged by size:
2573 2274 2167 1156 1109 834 827 782
554 33 13
BmgBI CAC'GTC
Cuts at: 1318 4289 4786 1318
Size: 2971 497 8854
Fragments arranged by size:
8854 2971 497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2282 3556 4207
Size: 416 53 83 399 24 865 1274 651
Cuts at: 4207 4290 4584 6590 8162 8187 8867 9562 10053
Size: 83 294 2006 1572 25 680 695 491
Cuts at: 10053 11011 442
Size: 958 1753
Fragments arranged by size:
2006 1753 1572 1274 958 865 695 680
651 491 416 399 294 83 83 53
25 24
BmtI G_CTAG'C
Cuts at: 1056 2930 1056
Size: 1874 10448
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2457 2511 7174 8454 8885 8887 2457
Size: 54 4663 1280 431 2 5892
Fragments arranged by size:
5892 4663 1280 431 54 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 2540 3149 3263 3272 3438 3740 4817
Size: 1034 267 609 114 9 166 302 1077
Cuts at: 4817 4833 5346 5390 6485 6497 7070 7091 7315
Size: 16 513 44 1095 12 573 21 224
Cuts at: 7315 8586 9401 9598 10982 1239
Size: 1271 815 197 1384 2579
Fragments arranged by size:
2579 1384 1271 1095 1077 1034 815 609
573 513 302 267 224 197 166 114
44 21 16 12 9
Bpu10I CC'TnA_GC
Cuts at: 2115 2913 2987 3462 3470 3604 3804 3864 4150
Size: 798 74 475 8 134 200 60 286
Cuts at: 4150 5219 6356 6996 8117 2115
Size: 1069 1137 640 1121 6320
Fragments arranged by size:
6320 1137 1121 1069 798 640 475 286
200 134 74 60 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 2552 3377 6640 8296 10447 11312 11610 2330
Size: 222 825 3263 1656 2151 865 298 3042
Fragments arranged by size:
3263 3042 2151 1656 865 825 298 222
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2372 4809 7268 8170 10985 882
Size: 617 873 2437 2459 902 2815 2219
Fragments arranged by size:
2815 2459 2437 2219 902 873 617
BsaAI yAC'GTr
Cuts at: 493 1204 2407 5041 8071 8545 10113 493
Size: 711 1203 2634 3030 474 1568 2702
Fragments arranged by size:
3030 2702 2634 1568 1203 711 474
BsaBI GATnn'nnATC
Cuts at: 7291 12316 7291
Size: 5025 7297
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7498 10129 10511 11146
Size: 53 83 186 936 5965 2631 382 635
Cuts at: 11146 275
Size: 1451
Fragments arranged by size:
5965 2631 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2298 2896 7435 10755 11153 11427 11574 11985 2298
Size: 598 4539 3320 398 274 147 411 2635
Fragments arranged by size:
4539 3320 2635 598 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2246 2276 3542 3572 3617
Size: 30 165 30 843 30 1266 30 45
Cuts at: 3617 3647 6616 6646 1178
Size: 30 2969 30 6854
Fragments arranged by size:
6854 2969 1266 843 165 45 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2381 2433 2549 2786 3197 3625 4268 5178
Size: 1195 52 116 237 411 428 643 910
Cuts at: 5178 5310 5393 5734 5863 5899 6637 7308 7649
Size: 132 83 341 129 36 738 671 341
Cuts at: 7649 7766 9326 1186
Size: 117 1560 4182
Fragments arranged by size:
4182 1560 1195 910 738 671 643 428
411 341 341 237 132 129 117 116
83 52 36
BseYI C'CCAG_C
Cuts at: 2499 2950 3426 5213 5256 5775 5896 5999 7377
Size: 451 476 1787 43 519 121 103 1378
Cuts at: 7377 7402 7799 8703 9053 9139 11525 12021 2499
Size: 25 397 904 350 86 2386 496 2800
Fragments arranged by size:
2800 2386 1787 1378 904 519 496 476
451 397 350 121 103 86 43 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2415 2780 3158 3715 4427 5200 5405
Size: 573 679 365 378 557 712 773 205
Cuts at: 5405 7118 7552 7817 8232 8342 8489 8561 9536
Size: 1713 434 265 415 110 147 72 975
Cuts at: 9536 1163
Size: 3949
Fragments arranged by size:
3949 1713 975 773 712 679 573 557
434 415 378 365 265 205 147 110
72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9772 10533 11561 1540
Size: 275 186 7771 761 1028 2301
Fragments arranged by size:
7771 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2475 2526 3207 3339 3468 4394 4659 5519 5579
Size: 51 681 132 129 926 265 860 60
Cuts at: 5579 5828 7479 7875 10383 10468 11539 2475
Size: 249 1651 396 2508 85 1071 3258
Fragments arranged by size:
3258 2508 1651 1071 926 860 681 396
265 249 132 129 85 60 51
BsmI GAATG_Cn'
Cuts at: 2075 2581 5061 8495 9833 9926 2075
Size: 506 2480 3434 1338 93 4471
Fragments arranged by size:
4471 3434 2480 1338 506 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2453 2453
Size: 12322
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2475 2526 2624 2637 2668 3191 3207
Size: 399 685 51 98 13 31 523 16
Cuts at: 3207 3238 3339 3468 3833 3863 4394 4659 4872
Size: 31 101 129 365 30 531 265 213
Cuts at: 4872 5313 5465 5519 5579 5706 5828 6132 6292
Size: 441 152 54 60 127 122 304 160
Cuts at: 6292 7401 7479 7875 9568 9601 9641 10383 10468
Size: 1109 78 396 1693 33 40 742 85
Cuts at: 10468 11539 1391
Size: 1071 2174
Fragments arranged by size:
2174 1693 1109 1071 742 685 531 523
441 399 396 365 304 265 213 160
152 129 127 122 101 98 85 78
60 54 51 40 33 31 31 30
16 13
BspEI T'CCGG_A
Cuts at: 2298 11985 2298
Size: 9687 2635
BspHI T'CATG_A
Cuts at: 6654 6784 7938 8466 10211 11941 6654
Size: 130 1154 528 1745 1730 7035
Fragments arranged by size:
7035 1745 1730 1154 528 130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4973 6046 6550 8511 9502 9677 844
Size: 4129 1073 504 1961 991 175 3489
Fragments arranged by size:
4129 3489 1961 1073 991 504 175
BsrBI CCG'CTC
Cuts at: 1394 1823 2776 2917 4722 10209 1394
Size: 429 953 141 1805 5487 3507
Fragments arranged by size:
5487 3507 1805 953 429 141
BsrDI GCAATG_nn'
Cuts at: 65 1248 2603 2928 3743 6225 7815 7845 10817
Size: 1183 1355 325 815 2482 1590 30 2972
Cuts at: 10817 10991 65
Size: 174 1396
Fragments arranged by size:
2972 2482 1590 1396 1355 1183 815 325
174 30
BsrFI r'CCGG_y
Cuts at: 1700 1810 7521 7982 10966 11153 1700
Size: 110 5711 461 2984 187 2869
Fragments arranged by size:
5711 2984 2869 461 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 12322
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12322
BssSI C'ACGA_G
Cuts at: 7587 10382 11394 7587
Size: 2795 1012 8515
Fragments arranged by size:
8515 2795 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3449 6805 7464 7539 8220 794
Size: 2655 3356 659 75 681 4896
Fragments arranged by size:
4896 3356 2655 681 659 75
BstBI TT'CG_AA
Cuts at: 2596 11126 2596
Size: 8530 3792
BstEII G'GTnAC_C
Cuts at: 1184 7856 1184
Size: 6672 5650
BstXI CCAn_nnnn'nTGG
Cuts at: 4337 6920 4337
Size: 2583 9739
BstYI r'GATC_y
Cuts at: 1972 2479 2908 3295 5388 6971 7267 7741 8088
Size: 507 429 387 2093 1583 296 474 347
Cuts at: 8088 8904 9267 9652 10019 10404 10421 11873 11959
Size: 816 363 385 367 385 17 1452 86
Cuts at: 11959 11971 12317 1972
Size: 12 346 1977
Fragments arranged by size:
2093 1977 1583 1452 816 507 474 429
387 385 385 367 363 347 346 296
86 17 12
BstZ17I GTA'TAC
Cuts at: 7337 7337
Size: 12322
Bsu36I CC'TnA_GG
Cuts at: 2486 3209 3374 3792 3835 4070 8399 2486
Size: 723 165 418 43 235 4329 6409
Fragments arranged by size:
6409 4329 723 418 235 165 43
BtgI C'CryG_G
Cuts at: 513 1065 4917 5174 9601 513
Size: 552 3852 257 4427 3234
Fragments arranged by size:
4427 3852 3234 552 257
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 11583
Fragments arranged by size:
11583 683 56
BtsI GCAGTG_nn'
Cuts at: 3130 4508 4720 4872 5791 6174 7160 7873 8241
Size: 1378 212 152 919 383 986 713 368
Cuts at: 8241 8451 9838 10632 10652 3130
Size: 210 1387 794 20 4800
Fragments arranged by size:
4800 1387 1378 986 919 794 713 383
368 212 210 152 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 9484 9519 529
Size: 35 8920 35 3332
Fragments arranged by size:
8920 3332 35 35
DraI TTT'AAA
Cuts at: 9626 9626
Size: 12322
DraIII CAC_nnn'GTG
Cuts at: 2393 3334 4228 5383 6831 2393
Size: 941 894 1155 1448 7884
Fragments arranged by size:
7884 1448 1155 941 894
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6509 11329 809
Size: 507 5193 4820 1802
Fragments arranged by size:
5193 4820 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2830 3672 4339
Size: 54 1425 50 275 18 1000 842 667
Cuts at: 4339 4429 4717 6025 6270 6876 6894 7519 7984
Size: 90 288 1308 245 606 18 625 465
Cuts at: 7984 8526 9769 10658 12287 8
Size: 542 1243 889 1629 43
Fragments arranged by size:
1629 1425 1308 1243 1000 889 842 667
625 606 542 465 288 275 245 90
54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 9769 1537
Size: 275 7957 4090
Fragments arranged by size:
7957 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3662 3821 5584 6690 6772 8501 8577 10252
Size: 2309 159 1763 1106 82 1729 76 1675
Cuts at: 10252 1353
Size: 3423
Fragments arranged by size:
3423 2309 1763 1729 1675 1106 159 82
76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4292 6566 9357 10909 11283 11429
Size: 1064 138 2703 2274 2791 1552 374 146
Cuts at: 11429 387
Size: 1280
Fragments arranged by size:
2791 2703 2274 1552 1280 1064 374 146
138
EcoICRI GAG'CTC
Cuts at: 2524 3205 5517 5577 5826 2524
Size: 681 2312 60 249 9020
Fragments arranged by size:
9020 2312 681 249 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2273 2540 3149 3263 3272 3438
Size: 393 518 123 267 609 114 9 166
Cuts at: 3438 3740 4598 4817 4833 5069 5183 5261 5346
Size: 302 858 219 16 236 114 78 85
Cuts at: 5346 5390 5455 6278 6324 6485 6497 7070 7091
Size: 44 65 823 46 161 12 573 21
Cuts at: 7091 7315 7626 8294 8475 8586 8812 8921 8941
Size: 224 311 668 181 111 226 109 20
Cuts at: 8941 8990 9401 9598 10385 10982 11769 12037 1239
Size: 49 411 197 787 597 787 268 1524
Fragments arranged by size:
1524 858 823 787 787 668 609 597
573 518 411 393 311 302 268 267
236 226 224 219 197 181 166 161
123 114 114 111 109 85 78 65
49 46 44 21 20 16 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2531 2723 2768 3377 3590 4069 4886 5222 5878
Size: 192 45 609 213 479 817 336 656
Cuts at: 5878 9392 2531
Size: 3514 5461
Fragments arranged by size:
5461 3514 817 656 609 479 336 213
192 45
EcoO109I rG'GnC_Cy
Cuts at: 2512 2620 2621 2812 3093 3180 3187 3247 3421
Size: 108 1 191 281 87 7 60 174
Cuts at: 3421 3595 3846 3980 4062 5168 5272 6289 6640
Size: 174 251 134 82 1106 104 1017 351
Cuts at: 6640 7795 7894 8358 9057 9385 2512
Size: 1155 99 464 699 328 5449
Fragments arranged by size:
5449 1155 1106 1017 699 464 351 328
281 251 191 174 174 134 108 104
99 87 82 60 7 1
EcoRI G'AATT_C
Cuts at: 3086 9363 9631 10009 3086
Size: 6277 268 378 5399
Fragments arranged by size:
6277 5399 378 268
EcoRV GAT'ATC
Cuts at: 2140 2140
Size: 12322
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2180 8435 2180
Size: 6255 6067
FspI TGC'GCA
Cuts at: 10828 10828
Size: 12322
HaeII r_GCGC'y
Cuts at: 1678 4621 4749 6079 6962 7806 7962 11469 1678
Size: 2943 128 1330 883 844 156 3507 2531
Fragments arranged by size:
3507 2943 2531 1330 883 844 156 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2431 2463 3314 3346 3463
Size: 32 135 32 859 32 851 32 117
Cuts at: 3463 3495 3572 3587 3604 3617 3619 3649 4806
Size: 32 77 15 17 13 2 30 1157
Cuts at: 4806 4838 5113 5145 5380 5412 6629 6661 7205
Size: 32 275 32 235 32 1217 32 544
Cuts at: 7205 7237 7726 7758 10963 10995 11037 11069 1373
Size: 32 489 32 3205 32 42 32 2626
Fragments arranged by size:
3205 2626 1217 1157 859 851 544 489
275 235 135 117 77 42 32 32
32 32 32 32 32 32 32 32
32 32 32 30 17 15 13 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3683 5027 9666 9912 669
Size: 988 281 1745 1344 4639 246 3079
Fragments arranged by size:
4639 3079 1745 1344 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2306 748
Size: 1558 10764
HpaI GTT'AAC
Cuts at: 9912 9912
Size: 12322
KpnI G_GTAC'C
Cuts at: 7436 9647 7436
Size: 2211 10111
MfeI C'AATT_G
Cuts at: 9921 9921
Size: 12322
MluI A'CGCG_T
Cuts at: 12198 12198
Size: 12322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4295 6964 7102 8653 8714 11436 11620 1305
Size: 2990 2669 138 1551 61 2722 184 2007
Fragments arranged by size:
2990 2722 2669 2007 1551 184 138 61
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2832 6272 6878 6896 8528
Size: 54 1425 343 1000 3440 606 18 1632
Cuts at: 8528 10
Size: 3804
Fragments arranged by size:
3804 3440 1632 1425 1000 606 343 54
18
MslI CAynn'nnrTG
Cuts at: 518 1166 2104 2838 4484 4826 6305 6659 6918
Size: 648 938 734 1646 342 1479 354 259
Cuts at: 6918 7073 9113 9408 10280 10639 10798 518
Size: 155 2040 295 872 359 159 2042
Fragments arranged by size:
2042 2040 1646 1479 938 872 734 648
359 354 342 295 259 159 155
MspA1I CmG'CkG
Cuts at: 1606 2787 5691 7057 7114 7515 7864 7948 8102
Size: 1181 2904 1366 57 401 349 84 154
Cuts at: 8102 8299 8556 8978 9010 9202 9537 9738 10415
Size: 197 257 422 32 192 335 201 677
Cuts at: 10415 11563 11808 1606
Size: 1148 245 2120
Fragments arranged by size:
2904 2120 1366 1181 1148 677 422 401
349 335 257 245 201 197 192 154
84 57 32
NaeI GCC'GGC
Cuts at: 7523 7984 7523
Size: 461 11861
NcoI C'CATG_G
Cuts at: 513 1065 4917 9601 513
Size: 552 3852 4684 3234
Fragments arranged by size:
4684 3852 3234 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 12322
NgoMIV G'CCGG_C
Cuts at: 7521 7982 7521
Size: 461 11861
NheI G'CTAG_C
Cuts at: 1052 2926 1052
Size: 1874 10448
NotI GC'GGCC_GC
Cuts at: 9769 9769
Size: 12322
NsiI A_TGCA'T
Cuts at: 6418 6418
Size: 12322
NspI r_CATG'y
Cuts at: 1132 1229 2109 6375 8207 9680 11225 1132
Size: 97 880 4266 1832 1473 1545 2229
Fragments arranged by size:
4266 2229 1832 1545 1473 880 97
PasI CC'CwG_GG
Cuts at: 1348 2639 3184 3907 3977 1348
Size: 1291 545 723 70 9693
Fragments arranged by size:
9693 1291 723 545 70
PciI A'CATG_T
Cuts at: 11221 11221
Size: 12322
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3065 4642 6279 7999 8131 9244 9379 1108
Size: 1957 1577 1637 1720 132 1113 135 4051
Fragments arranged by size:
4051 1957 1720 1637 1577 1113 135 132
PfoI T'CCnGG_A
Cuts at: 3249 4833 9614 3249
Size: 1584 4781 5957
Fragments arranged by size:
5957 4781 1584
PmeI GTTT'AAAC
Cuts at: 9626 9626
Size: 12322
PmlI CAC'GTG
Cuts at: 2407 8071 2407
Size: 5664 6658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10391 10423 11904 11936 10391
Size: 32 1481 32 10777
Fragments arranged by size:
10777 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3180 3247 3421 3595 3846 5272 6640 9057 3180
Size: 67 174 174 251 1426 1368 2417 6445
Fragments arranged by size:
6445 2417 1426 1368 251 174 174 67
PshAI GACnn'nnGTC
Cuts at: 1613 4905 6935 1613
Size: 3292 2030 7000
Fragments arranged by size:
7000 3292 2030
PsiI TTA'TAA
Cuts at: 9892 9892
Size: 12322
PspOMI G'GGCC_C
Cuts at: 2620 3187 6288 2620
Size: 567 3101 8654
Fragments arranged by size:
8654 3101 567
PspXI vC'TCGA_Gb
Cuts at: 2363 3008 2363
Size: 645 11677
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 8462 8494 8462
Size: 32 12290
PstI C_TGCA'G
Cuts at: 830 2574 2747 4173 4753 5483 5497 6052 6561
Size: 1744 173 1426 580 730 14 555 509
Cuts at: 6561 7267 7472 7574 7883 8107 8778 8977 9015
Size: 706 205 102 309 224 671 199 38
Cuts at: 9015 9192 9227 9674 830
Size: 177 35 447 3478
Fragments arranged by size:
3478 1744 1426 730 706 671 580 555
509 447 309 224 205 199 177 173
102 38 35 14
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12322
PvuII CAG'CTG
Cuts at: 2787 5691 7057 7114 7864 7948 8102 8299 8556
Size: 2904 1366 57 750 84 154 197 257
Cuts at: 8556 8978 9010 9202 2787
Size: 422 32 192 5907
Fragments arranged by size:
5907 2904 1366 750 422 257 197 192
154 84 57 32
SacI G_AGCT'C
Cuts at: 2526 3207 5519 5579 5828 2526
Size: 681 2312 60 249 9020
Fragments arranged by size:
9020 2312 681 249 60
SalI G'TCGA_C
Cuts at: 1936 5025 9664 1936
Size: 3089 4639 4594
Fragments arranged by size:
4639 4594 3089
SapI GCTCTTCn'nnn_
Cuts at: 3821 5584 8577 3821
Size: 1763 2993 7566
Fragments arranged by size:
7566 2993 1763
SbfI CC_TGCA'GG
Cuts at: 2747 9674 2747
Size: 6927 5395
ScaI AGT'ACT
Cuts at: 8144 8714 10570 12282 8144
Size: 570 1856 1712 8184
Fragments arranged by size:
8184 1856 1712 570
SexAI A'CCwGG_T
Cuts at: 9026 9026
Size: 12322
SfcI C'TryA_G
Cuts at: 826 1045 2120 2570 2743 2874 3609 4169 4730
Size: 219 1075 450 173 131 735 560 561
Cuts at: 4730 4749 5479 5493 6048 6440 6536 6557 7263
Size: 19 730 14 555 392 96 21 706
Cuts at: 7263 7468 7570 7606 7879 8103 8512 8774 8973
Size: 205 102 36 273 224 409 262 199
Cuts at: 8973 9011 9188 9223 9670 10805 11486 11677 826
Size: 38 177 35 447 1135 681 191 1471
Fragments arranged by size:
1471 1135 1075 735 730 706 681 561
560 555 450 447 409 392 273 262
224 219 205 199 191 177 173 131
102 96 38 36 35 21 19 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12322
SmaI CCC'GGG
Cuts at: 1872 2270 6065 6287 9593 9649 1872
Size: 398 3795 222 3306 56 4545
Fragments arranged by size:
4545 3795 3306 398 222 56
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2309 2363 2531 3008 3392 5927
Size: 197 926 366 54 168 477 384 2535
Cuts at: 5927 6619 8311 8882 10426 11327 11589 820
Size: 692 1692 571 1544 901 262 1553
Fragments arranged by size:
2535 1692 1553 1544 926 901 692 571
477 384 366 262 197 168 54
SnaBI TAC'GTA
Cuts at: 493 10113 493
Size: 9620 2702
SpeI A'CTAG_T
Cuts at: 152 4019 152
Size: 3867 8455
SphI G_CATG'C
Cuts at: 1132 8207 9680 1132
Size: 7075 1473 3774
Fragments arranged by size:
7075 3774 1473
SspI AAT'ATT
Cuts at: 5 52 8044 10246 5
Size: 47 7992 2202 2081
Fragments arranged by size:
7992 2202 2081 47
StuI AGG'CCT
Cuts at: 1517 2741 6107 1517
Size: 1224 3366 7732
Fragments arranged by size:
7732 3366 1224
StyI C'CwwG_G
Cuts at: 513 1065 2338 2423 3417 4194 4917 5879 5916
Size: 552 1273 85 994 777 723 962 37
Cuts at: 5916 6264 7890 8694 8889 9601 9761 513
Size: 348 1626 804 195 712 160 3074
Fragments arranged by size:
3074 1626 1273 994 962 804 777 723
712 552 348 195 160 85 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2765 3789 4603 6926 10349 10366 10519
Size: 1255 1185 1024 814 2323 3423 17 153
Cuts at: 10519 10704 11149 325
Size: 185 445 1498
Fragments arranged by size:
3423 2323 1498 1255 1185 1024 814 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2248 3112 4373
Size: 274 80 33 51 1443 271 864 1261
Cuts at: 4373 6238 8142 8712 9258 10568 12280 96
Size: 1865 1904 570 546 1310 1712 138
Fragments arranged by size:
1904 1865 1712 1443 1310 1261 864 570
546 274 271 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3542 3574 6616 6648 12282 12314 3542
Size: 32 3042 32 5634 32 3550
Fragments arranged by size:
5634 3550 3042 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 12322
XbaI T'CTAG_A
Cuts at: 2905 6667 6769 7660 9658 2905
Size: 3762 102 891 1998 5569
Fragments arranged by size:
5569 3762 1998 891 102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2958 7552 7909 2958
Size: 4594 357 7371
Fragments arranged by size:
7371 4594 357
XhoI C'TCGA_G
Cuts at: 1943 2363 3008 1943
Size: 420 645 11257
Fragments arranged by size:
11257 645 420
XmaI C'CCGG_G
Cuts at: 1870 2268 6063 6285 9591 9647 1870
Size: 398 3795 222 3306 56 4545
Fragments arranged by size:
4545 3795 3306 398 222 56
XmnI GAAnn'nnTTC
Cuts at: 3387 5196 8571 10451 3387
Size: 1809 3375 1880 5258
Fragments arranged by size:
5258 3375 1880 1809
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10130 276
Size: 53 83 186 936 8596 2468
Fragments arranged by size:
8596 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BsiWI FseI FspAI KasI
NarI NruI PacI RsrII SacII SanDI SfiI SfoI
SrfI SwaI