Restriction map for
pFN21AA0318
(Circular) (Six-base) MAPSORT of: pFN21AA0318.seq Check: 6421 from: 1 to: 7861
pFN21AA0318 7861 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3201 3201
Size: 7861
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5671 278
Size: 53 83 186 936 4135 2468
Fragments arranged by size:
4135 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5204 1937
Size: 3267 4594
Acc65I G'GTAC_C
Cuts at: 5182 5182
Size: 7861
AclI AA'CG_TT
Cuts at: 1563 3573 5988 6361 1563
Size: 2010 2415 373 3063
Fragments arranged by size:
3063 2415 2010 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3636 5924 7308 7576 1632
Size: 2004 2288 1384 268 1917
Fragments arranged by size:
2288 2004 1917 1384 268
AfeI AGC'GCT
Cuts at: 2323 4432 2323
Size: 2109 5752
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7664
AflIII A'CryG_T
Cuts at: 1203 6760 7737 1203
Size: 5557 977 1327
Fragments arranged by size:
5557 1327 977
AgeI A'CCGG_T
Cuts at: 6692 6692
Size: 7861
AhdI GACnn_n'nnGTC
Cuts at: 2710 6590 2710
Size: 3880 3981
AleI CACnn'nnGTG
Cuts at: 1166 2517 3647 3992 1166
Size: 1351 1130 345 5035
Fragments arranged by size:
5035 1351 1130 345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3122 3906 1184
Size: 1938 784 5139
Fragments arranged by size:
5139 1938 784
AlwNI CAG_nnn'CTG
Cuts at: 2035 2059 3740 4385 4526 4613 5604 7176 2035
Size: 24 1681 645 141 87 991 1572 2720
Fragments arranged by size:
2720 1681 1572 991 645 141 87 24
ApaI G_GGCC'C
Cuts at: 3938 3938
Size: 7861
ApaLI G'TGCA_C
Cuts at: 3148 3891 5918 7074 3148
Size: 743 2027 1156 3935
Fragments arranged by size:
3935 2027 1156 743
ApoI r'AATT_y
Cuts at: 1493 2473 5170 5396 5548 7657 1493
Size: 980 2697 226 152 2109 1697
Fragments arranged by size:
2697 2109 1697 980 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6415 160
Size: 872 5383 1606
Fragments arranged by size:
5383 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7861
AvaI C'yCGr_G
Cuts at: 1870 1943 2169 2500 3752 3781 4023 5186 1870
Size: 73 226 331 1252 29 242 1163 4545
Fragments arranged by size:
4545 1252 1163 331 242 226 73 29
BamHI G'GATC_C
Cuts at: 5191 5558 5191
Size: 367 7494
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2674 2968 3763 3986 4160
Size: 325 217 627 887 294 795 223 174
Cuts at: 4160 4535 5182 6637 618
Size: 375 647 1455 1842
Fragments arranged by size:
1842 1455 887 795 647 627 375 325
294 223 217 174
BanII G_rGCy'C
Cuts at: 1391 2652 2961 3759 3938 4548 5180 1391
Size: 1261 309 798 179 610 632 4072
Fragments arranged by size:
4072 1261 798 632 610 309 179
BbeI G_GCGC'C
Cuts at: 4164 4164
Size: 7861
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2892 4069 928
Size: 971 993 1177 4720
Fragments arranged by size:
4720 1177 993 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2828 2862 6052 6086 1903
Size: 34 50 34 807 34 3190 34 3678
Fragments arranged by size:
3678 3190 807 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5753 6963 5753
Size: 1210 6651
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7861
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3553 4028 6472
Size: 107 122 71 631 605 1881 475 2444
Cuts at: 6472 136
Size: 1525
Fragments arranged by size:
2444 1881 1525 631 605 475 122 107
71
BglII A'GATC_T
Cuts at: 2444 3216 7856 2444
Size: 772 4640 2449
Fragments arranged by size:
4640 2449 772
BlpI GC'TnA_GC
Cuts at: 2574 5278 2574
Size: 2704 5157
Bme1580I G_kGCm'C
Cuts at: 1790 2679 3152 3895 3915 3938 3989 4708 5034
Size: 889 473 743 20 23 51 719 326
Cuts at: 5034 5922 7078 1790
Size: 888 1156 2573
Fragments arranged by size:
2573 1156 889 888 743 719 473 326
51 23 20
BmgBI CAC'GTC
Cuts at: 1318 2863 3172 3265 4347 1318
Size: 1545 309 93 1082 4832
Fragments arranged by size:
4832 1545 1082 309 93
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5592 6550 442
Size: 416 53 83 399 24 4175 958 1753
Fragments arranged by size:
4175 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7861
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2669 2669
Size: 7861
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2801 3425 3547 3695 3840 3941 4125
Size: 959 603 624 122 148 145 101 184
Cuts at: 4125 4485 6521 1239
Size: 360 2036 2579
Fragments arranged by size:
2579 2036 959 624 603 360 184 148
145 122 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4346 5088 5986 6851 7149 4346
Size: 742 898 865 298 5058
Fragments arranged by size:
5058 898 865 742 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3523 3526 3778 4482 4741 6524 882
Size: 617 2024 3 252 704 259 1783 2219
Fragments arranged by size:
2219 2024 1783 704 617 259 252 3
BsaAI yAC'GTr
Cuts at: 493 1204 5652 493
Size: 711 4448 2702
Fragments arranged by size:
4448 2702 711
BsaBI GATnn'nnATC
Cuts at: 2443 7855 2443
Size: 5412 2449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3545 4128 4161 4849
Size: 53 83 186 936 2012 583 33 688
Cuts at: 4849 5668 6050 6685 275
Size: 819 382 635 1451
Fragments arranged by size:
2012 1451 936 819 688 635 583 382
186 83 53 33
BsaWI w'CCGG_w
Cuts at: 2426 6294 6692 6966 7113 7524 2426
Size: 3868 398 274 147 411 2763
Fragments arranged by size:
3868 2763 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2381 2411 2792 2822 3086
Size: 30 165 30 978 30 381 30 264
Cuts at: 3086 3116 4280 4310 1178
Size: 30 1164 30 4729
Fragments arranged by size:
4729 1164 978 381 264 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2642 3296 3299 4136 4168 4292 4301 4304
Size: 1456 654 3 837 32 124 9 3
Cuts at: 4304 4634 4637 4775 1186
Size: 330 3 138 4272
Fragments arranged by size:
4272 1456 837 654 330 138 124 32
9 3 3 3
BseYI C'CCAG_C
Cuts at: 2215 2258 2731 2952 3040 3225 3463 3494 3959
Size: 43 473 221 88 185 238 31 465
Cuts at: 3959 4337 4378 7064 7560 2215
Size: 378 41 2686 496 2516
Fragments arranged by size:
2686 2516 496 473 465 378 238 221
185 88 43 41 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2720 3073 3128 3774 1163
Size: 573 984 353 55 646 5250
Fragments arranged by size:
5250 984 646 573 353 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2346 3648 3950 5311 6072 7100
Size: 275 186 345 1302 302 1361 761 1028
Cuts at: 7100 1540
Size: 2301
Fragments arranged by size:
2301 1361 1302 1028 761 345 302 275
186
BsiHKAI G_wGCw'C
Cuts at: 2652 2793 3152 3759 3846 3895 4548 5922 6007
Size: 141 359 607 87 49 653 1374 85
Cuts at: 6007 7078 2652
Size: 1071 3435
Fragments arranged by size:
3435 1374 1071 653 607 359 141 87
85 49
BsmI GAATG_Cn'
Cuts at: 5372 5465 5372
Size: 93 7768
BsmBI CGTCTCn'nnnn_
Cuts at: 4218 4218
Size: 7861
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2652 2679 2793 2961 3152 3759 3846
Size: 399 862 27 114 168 191 607 87
Cuts at: 3846 3895 3915 3938 3989 4548 4708 5034 5180
Size: 49 20 23 51 559 160 326 146
Cuts at: 5180 5922 6007 7078 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 862 742 607 559 399 326
191 168 160 146 114 87 85 51
49 27 23 20
BspEI T'CCGG_A
Cuts at: 7524 7524
Size: 7861
BspHI T'CATG_A
Cuts at: 2280 5750 7480 2280
Size: 3470 1730 2661
Fragments arranged by size:
3470 2661 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 2220 3201 3796 4047 5216 844
Size: 1325 51 981 595 251 1169 3489
Fragments arranged by size:
3489 1325 1169 981 595 251 51
BsrBI CCG'CTC
Cuts at: 1394 1823 4588 5748 1394
Size: 429 2765 1160 3507
Fragments arranged by size:
3507 2765 1160 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6356 6530 65
Size: 1183 5108 174 1396
Fragments arranged by size:
5108 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3578 6505 6692 1700
Size: 110 1768 2927 187 2869
Fragments arranged by size:
2927 2869 1768 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7861
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7861
BssSI C'ACGA_G
Cuts at: 5921 6933 5921
Size: 1012 6849
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2035 3740 794
Size: 1241 1705 4915
Fragments arranged by size:
4915 1705 1241
BstBI TT'CG_AA
Cuts at: 6665 6665
Size: 7861
BstEII G'GTnAC_C
Cuts at: 1184 3499 1184
Size: 2315 5546
BstXI CCAn_nnnn'nTGG
Cuts at: 2689 2979 4018 2689
Size: 290 1039 6532
Fragments arranged by size:
6532 1039 290
BstYI r'GATC_y
Cuts at: 1972 2423 2444 3216 4557 5191 5558 5943 5960
Size: 451 21 772 1341 634 367 385 17
Cuts at: 5960 7412 7498 7510 7856 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1341 772 634 451 385 367
346 86 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2250 3389 3630 3648 3723 3930 4017
Size: 552 1185 1139 241 18 75 207 87
Cuts at: 4017 4219 4423 4690 513
Size: 202 204 267 3684
Fragments arranged by size:
3684 1185 1139 552 267 241 207 204
202 87 75 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7122
Fragments arranged by size:
7122 683 56
BtsI GCAGTG_nn'
Cuts at: 3116 3152 5377 6171 6191 3116
Size: 36 2225 794 20 4786
Fragments arranged by size:
4786 2225 794 36 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7861
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7826
DraI TTT'AAA
Cuts at: 4636 5165 4636
Size: 529 7332
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5069 6868 809
Size: 507 3753 1799 1802
Fragments arranged by size:
3753 1802 1799 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2659 3369 3645
Size: 54 1425 50 275 18 829 710 276
Cuts at: 3645 4044 5308 6197 7826 8
Size: 399 1264 889 1629 43
Fragments arranged by size:
1629 1425 1264 889 829 710 399 276
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3645 5308 1537
Size: 275 1833 1663 4090
Fragments arranged by size:
4090 1833 1663 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 4509 4536 5135 5791 1353
Size: 768 2388 27 599 656 3423
Fragments arranged by size:
3423 2388 768 656 599 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2389 3211 3697 6448 6822 6968
Size: 1064 138 800 822 486 2751 374 146
Cuts at: 6968 387
Size: 1280
Fragments arranged by size:
2751 1280 1064 822 800 486 374 146
138
EcoICRI GAG'CTC
Cuts at: 2650 3757 4546 2650
Size: 1107 789 5965
Fragments arranged by size:
5965 1107 789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2801 3425 3547 3636 3695 3840
Size: 393 566 603 624 122 89 59 145
Cuts at: 3840 3941 4125 4485 5924 6521 7308 7576 1239
Size: 101 184 360 1439 597 787 268 1524
Fragments arranged by size:
1524 1439 787 624 603 597 566 393
360 268 184 145 122 101 89 59
EcoNI CCTnn'n_nnAGG
Cuts at: 3278 3278
Size: 7861
EcoO109I rG'GnC_Cy
Cuts at: 2056 2173 2853 3458 3851 3935 4446 2056
Size: 117 680 605 393 84 511 5471
Fragments arranged by size:
5471 680 605 511 393 117 84
EcoRI G'AATT_C
Cuts at: 2473 5170 5548 2473
Size: 2697 378 4786
Fragments arranged by size:
4786 2697 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 3445 5093 2428
Size: 1017 1648 5196
Fragments arranged by size:
5196 1648 1017
FspI TGC'GCA
Cuts at: 6367 6367
Size: 7861
HaeII r_GCGC'y
Cuts at: 1678 2325 4164 4434 7008 1678
Size: 647 1839 270 2574 2531
Fragments arranged by size:
2574 2531 1839 647 270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 2696 2728 2778
Size: 32 135 32 809 32 283 32 50
Cuts at: 2778 2792 2810 2824 2874 2906 4756 4788 5075
Size: 14 18 14 50 32 1850 32 287
Cuts at: 5075 5107 6502 6534 6576 6608 1373
Size: 32 1395 32 42 32 2626
Fragments arranged by size:
2626 1850 1395 809 287 283 135 50
50 42 32 32 32 32 32 32
32 32 32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 5205 5451 669
Size: 988 281 3267 246 3079
Fragments arranged by size:
3267 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7861
HpaI GTT'AAC
Cuts at: 5451 5451
Size: 7861
KasI G'GCGC_C
Cuts at: 4160 4160
Size: 7861
KpnI G_GTAC'C
Cuts at: 5186 5186
Size: 7861
MfeI C'AATT_G
Cuts at: 5460 5460
Size: 7861
MluI A'CGCG_T
Cuts at: 7737 7737
Size: 7861
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2022 2706 6975 7159 1305
Size: 717 684 4269 184 2007
Fragments arranged by size:
4269 2007 717 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2661 3371 10
Size: 54 1425 343 829 710 4500
Fragments arranged by size:
4500 1425 829 710 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2517 2823 2977 3104 3647 3992 5819
Size: 648 1351 306 154 127 543 345 1827
Cuts at: 5819 6178 6337 518
Size: 359 159 2042
Fragments arranged by size:
2042 1827 1351 648 543 359 345 306
159 154 127
MspA1I CmG'CkG
Cuts at: 1606 2035 3403 4312 4341 5277 5954 7102 7347
Size: 429 1368 909 29 936 677 1148 245
Cuts at: 7347 1606
Size: 2120
Fragments arranged by size:
2120 1368 1148 936 909 677 429 245
29
NarI GG'CG_CC
Cuts at: 4161 4161
Size: 7861
NcoI C'CATG_G
Cuts at: 513 1065 4017 4219 513
Size: 552 2952 202 4155
Fragments arranged by size:
4155 2952 552 202
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7861
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7861
NotI GC'GGCC_GC
Cuts at: 5308 5308
Size: 7861
NspI r_CATG'y
Cuts at: 1132 1229 3919 5219 6764 1132
Size: 97 2690 1300 1545 2229
Fragments arranged by size:
2690 2229 1545 1300 97
PasI CC'CwG_GG
Cuts at: 1348 3712 1348
Size: 2364 5497
PciI A'CATG_T
Cuts at: 6760 6760
Size: 7861
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2059 1108
Size: 951 6910
PfoI T'CCnGG_A
Cuts at: 2796 3524 2796
Size: 728 7133
PmeI GTTT'AAAC
Cuts at: 5165 5165
Size: 7861
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2618 2650 3272 3304 4367 4399 5930 5962 7443
Size: 32 622 32 1063 32 1531 32 1481
Cuts at: 7443 7475 2618
Size: 32 3004
Fragments arranged by size:
3004 1531 1481 1063 622 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2173 2853 3458 3851 4446 2173
Size: 680 605 393 595 5588
Fragments arranged by size:
5588 680 605 595 393
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7861
PsiI TTA'TAA
Cuts at: 5431 5431
Size: 7861
PspOMI G'GGCC_C
Cuts at: 3934 3934
Size: 7861
PstI C_TGCA'G
Cuts at: 830 2040 2103 3147 3457 3736 4390 4618 5213
Size: 1210 63 1044 310 279 654 228 595
Cuts at: 5213 830
Size: 3478
Fragments arranged by size:
3478 1210 1044 654 595 310 279 228
63
PvuI CG_AT'CG
Cuts at: 2001 2346 2001
Size: 345 7516
PvuII CAG'CTG
Cuts at: 2035 4312 2035
Size: 2277 5584
SacI G_AGCT'C
Cuts at: 2652 3759 4548 2652
Size: 1107 789 5965
Fragments arranged by size:
5965 1107 789
SalI G'TCGA_C
Cuts at: 1936 5203 1936
Size: 3267 4594
SanDI GG'GwC_CC
Cuts at: 2853 3851 2853
Size: 998 6863
SapI GCTCTTCn'nnn_
Cuts at: 4536 4536
Size: 7861
SbfI CC_TGCA'GG
Cuts at: 3457 5213 3457
Size: 1756 6105
ScaI AGT'ACT
Cuts at: 6109 7821 6109
Size: 1712 6149
SfcI C'TryA_G
Cuts at: 826 1045 2036 2099 2530 3143 3256 3453 3732
Size: 219 991 63 431 613 113 197 279
Cuts at: 3732 4386 4614 5209 6344 7025 7216 826
Size: 654 228 595 1135 681 191 1471
Fragments arranged by size:
1471 1135 991 681 654 613 595 431
279 228 219 197 191 113 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 4028 4028
Size: 7861
SfoI GGC'GCC
Cuts at: 4162 4162
Size: 7861
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7861
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7861
SmaI CCC'GGG
Cuts at: 1872 2171 4025 5188 1872
Size: 299 1854 1163 4545
Fragments arranged by size:
4545 1854 1163 299
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4361 5103 5965 6866 7128 820
Size: 197 926 2418 742 862 901 262 1553
Fragments arranged by size:
2418 1553 926 901 862 742 262 197
SnaBI TAC'GTA
Cuts at: 493 5652 493
Size: 5159 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7861
SphI G_CATG'C
Cuts at: 1132 5219 1132
Size: 4087 3774
SspI AAT'ATT
Cuts at: 5 52 5785 5
Size: 47 5733 2081
Fragments arranged by size:
5733 2081 47
StuI AGG'CCT
Cuts at: 1517 3428 3875 5025 1517
Size: 1911 447 1150 4353
Fragments arranged by size:
4353 1911 1150 447
StyI C'CwwG_G
Cuts at: 513 1065 2109 3681 4011 4017 4219 5300 513
Size: 552 1044 1572 330 6 202 1081 3074
Fragments arranged by size:
3074 1572 1081 1044 552 330 202 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2207 2569 3973 5888 5905 6058 6243
Size: 1255 627 362 1404 1915 17 153 185
Cuts at: 6243 6688 325
Size: 445 1498
Fragments arranged by size:
1915 1498 1404 1255 627 445 362 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6107 7819 96
Size: 274 80 33 51 1443 4130 1712 138
Fragments arranged by size:
4130 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2131 2163 5128 5160 7821 7853 2131
Size: 32 2965 32 2661 32 2139
Fragments arranged by size:
2965 2661 2139 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3296 1316
Size: 1980 5881
XbaI T'CTAG_A
Cuts at: 5197 5197
Size: 7861
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7861
XmaI C'CCGG_G
Cuts at: 1870 2169 4023 5186 1870
Size: 299 1854 1163 4545
Fragments arranged by size:
4545 1854 1163 299
XmnI GAAnn'nnTTC
Cuts at: 5990 5990
Size: 7861
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5669 276
Size: 53 83 186 936 4135 2468
Fragments arranged by size:
4135 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SexAI SrfI SwaI XcmI