Restriction map for
pFN21AA0311
(Circular) (Six-base) MAPSORT of: pFN21AA0311.seq Check: 3985 from: 1 to: 11662
pFN21AA0311 11662 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5407 9472 278
Size: 53 83 186 936 3871 4065 2468
Fragments arranged by size:
4065 3871 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2907 4491 4814 5566 9005 1937
Size: 970 1584 323 752 3439 4594
Fragments arranged by size:
4594 3439 1584 970 752 323
Acc65I G'GTAC_C
Cuts at: 8983 8983
Size: 11662
AclI AA'CG_TT
Cuts at: 1563 9789 10162 1563
Size: 8226 373 3063
Fragments arranged by size:
8226 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2586 2973 4595 7317 8802 9725 11109 11377
Size: 954 387 1622 2722 1485 923 1384 268
Cuts at: 11377 1632
Size: 1917
Fragments arranged by size:
2722 1917 1622 1485 1384 954 923 387
268
AflII C'TTAA_G
Cuts at: 820 1017 3914 820
Size: 197 2897 8568
Fragments arranged by size:
8568 2897 197
AflIII A'CryG_T
Cuts at: 1203 5561 6225 7567 10561 11538 1203
Size: 4358 664 1342 2994 977 1327
Fragments arranged by size:
4358 2994 1342 1327 977 664
AgeI A'CCGG_T
Cuts at: 10493 10493
Size: 11662
AhdI GACnn_n'nnGTC
Cuts at: 4939 10391 4939
Size: 5452 6210
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 11662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2385 8429 1184
Size: 1201 6044 4417
Fragments arranged by size:
6044 4417 1201
AlwNI CAG_nnn'CTG
Cuts at: 2050 2189 2876 4037 4294 7184 8715 9405 10977
Size: 139 687 1161 257 2890 1531 690 1572
Cuts at: 10977 2050
Size: 2735
Fragments arranged by size:
2890 2735 1572 1531 1161 690 687 257
139
ApaI G_GGCC'C
Cuts at: 4152 4152
Size: 11662
ApaLI G'TGCA_C
Cuts at: 9719 10875 9719
Size: 1156 10506
ApoI r'AATT_y
Cuts at: 1493 3419 3435 3643 4027 4373 6079 6239 7356
Size: 1926 16 208 384 346 1706 160 1117
Cuts at: 7356 7597 7856 8971 9197 9349 11458 1493
Size: 241 259 1115 226 152 2109 1697
Fragments arranged by size:
2109 1926 1706 1697 1117 1115 384 346
259 241 226 208 160 152 16
AseI AT'TA_AT
Cuts at: 160 1032 5127 10216 160
Size: 872 4095 5089 1606
Fragments arranged by size:
5089 4095 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11662
AvaI C'yCGr_G
Cuts at: 1870 1943 8434 8987 1870
Size: 73 6491 553 4545
Fragments arranged by size:
6491 4545 553 73
AvrII C'CTAG_G
Cuts at: 5158 5158
Size: 11662
BamHI G'GATC_C
Cuts at: 2053 6490 8992 9359 2053
Size: 4437 2502 367 4356
Fragments arranged by size:
4437 4356 2502 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2457 3517 3787 8983 10438
Size: 325 217 627 670 1060 270 5196 1455
Cuts at: 10438 618
Size: 1842
Fragments arranged by size:
5196 1842 1455 1060 670 627 325 270
217
BanII G_rGCy'C
Cuts at: 1391 3584 4152 4660 4917 6938 7095 8981 1391
Size: 2193 568 508 257 2021 157 1886 4072
Fragments arranged by size:
4072 2193 2021 1886 568 508 257 157
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2937 3324 7056 7371 928
Size: 971 1038 387 3732 315 5219
Fragments arranged by size:
5219 3732 1038 971 387 315
BbvCI CC'TCA_GC
Cuts at: 4737 8670 4737
Size: 3933 7729
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2447 2481 5387 5421 8231 8265 9853
Size: 34 510 34 2906 34 2810 34 1588
Cuts at: 9853 9887 1903
Size: 34 3678
Fragments arranged by size:
3678 2906 2810 1588 510 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4657 9554 10764 4657
Size: 4897 1210 5555
Fragments arranged by size:
5555 4897 1210
BclI T'GATC_A
Cuts at: 1548 2350 5499 5719 1548
Size: 802 3149 220 7491
Fragments arranged by size:
7491 3149 802 220
BfrBI ATG'CAT
Cuts at: 7151 8956 7151
Size: 1805 9857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 10273 136
Size: 107 122 71 631 605 8601 1525
Fragments arranged by size:
8601 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6016 6826 6904 11657 6016
Size: 810 78 4753 6021
Fragments arranged by size:
6021 4753 810 78
BlpI GC'TnA_GC
Cuts at: 3583 9079 3583
Size: 5496 6166
Bme1580I G_kGCm'C
Cuts at: 1790 4152 5193 9723 10879 1790
Size: 2362 1041 4530 1156 2573
Fragments arranged by size:
4530 2573 2362 1156 1041
BmgBI CAC'GTC
Cuts at: 1318 3844 5564 1318
Size: 2526 1720 7416
Fragments arranged by size:
7416 2526 1720
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2981 4018 9393
Size: 416 53 83 399 24 1564 1037 5375
Cuts at: 9393 10351 442
Size: 958 1753
Fragments arranged by size:
5375 1753 1564 1037 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11662
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8509 8509
Size: 11662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4481 5273 6705 6758 7258 7318 10322 1239
Size: 3242 792 1432 53 500 60 3004 2579
Fragments arranged by size:
3242 3004 2579 1432 792 500 60 53
Bpu10I CC'TnA_GC
Cuts at: 2802 4737 8670 2802
Size: 1935 3933 5794
Fragments arranged by size:
5794 3933 1935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2874 3686 4492 4493 4565 4778 9787 10652 10950
Size: 812 806 1 72 213 5009 865 298
Cuts at: 10950 2874
Size: 3586
Fragments arranged by size:
5009 3586 865 812 806 298 213 72
1
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2219 2253 7535 10325 882
Size: 617 720 34 5282 2790 2219
Fragments arranged by size:
5282 2790 2219 720 617 34
BsaAI yAC'GTr
Cuts at: 493 1204 5210 9453 493
Size: 711 4006 4243 2702
Fragments arranged by size:
4243 4006 2702 711
BsaBI GATnn'nnATC
Cuts at: 2310 11656 2310
Size: 9346 2316
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2517 5404 9469 9851
Size: 53 83 186 936 984 2887 4065 382
Cuts at: 9851 10486 275
Size: 635 1451
Fragments arranged by size:
4065 2887 1451 984 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2235 3784 8771 10095 10493 10767 10914 11325 2235
Size: 1549 4987 1324 398 274 147 411 2572
Fragments arranged by size:
4987 2572 1549 1324 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2764 2794 3860 3890 6204
Size: 30 165 30 1361 30 1066 30 2314
Cuts at: 6204 6234 7256 7286 8270 8300 1178
Size: 30 1022 30 984 30 4540
Fragments arranged by size:
4540 2314 1361 1066 1022 984 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 2894 3013 3046 3820 3996 4190 4538
Size: 1255 453 119 33 774 176 194 348
Cuts at: 4538 4994 5424 5807 6078 8517 8519 1186
Size: 456 430 383 271 2439 2 4329
Fragments arranged by size:
4329 2439 1255 774 456 453 430 383
348 271 194 176 119 33 2
BseYI C'CCAG_C
Cuts at: 8549 8888 10865 11361 8549
Size: 339 1977 496 8850
Fragments arranged by size:
8850 1977 496 339
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 3056 7354 8739 1163
Size: 573 729 591 4298 1385 4086
Fragments arranged by size:
4298 4086 1385 729 591 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9112 9873 10901 1540
Size: 275 186 7111 761 1028 2301
Fragments arranged by size:
7111 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3584 4507 4917 7095 8527 9723 9808 10879 3584
Size: 923 410 2178 1432 1196 85 1071 4367
Fragments arranged by size:
4367 2178 1432 1196 1071 923 410 85
BsmI GAATG_Cn'
Cuts at: 2496 2675 3697 5479 6673 7153 8293 8468 8799
Size: 179 1022 1782 1194 480 1140 175 331
Cuts at: 8799 9173 9266 2496
Size: 374 93 4892
Fragments arranged by size:
4892 1782 1194 1140 1022 480 374 331
179 175 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3584 4152 4507 4660 4917 5193 6938
Size: 399 1794 568 355 153 257 276 1745
Cuts at: 6938 7095 8527 8981 9723 9808 10879 1391
Size: 157 1432 454 742 85 1071 2174
Fragments arranged by size:
2174 1794 1745 1432 1071 742 568 454
399 355 276 257 157 153 85
BspEI T'CCGG_A
Cuts at: 2235 8771 11325 2235
Size: 6536 2554 2572
Fragments arranged by size:
6536 2572 2554
BspHI T'CATG_A
Cuts at: 3064 6604 8356 8932 9551 11281 3064
Size: 3540 1752 576 619 1730 3445
Fragments arranged by size:
3540 3445 1752 1730 619 576
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5223 7275 9017 844
Size: 4379 2052 1742 3489
Fragments arranged by size:
4379 3489 2052 1742
BsrBI CCG'CTC
Cuts at: 1394 1823 9549 1394
Size: 429 7726 3507
Fragments arranged by size:
7726 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2136 3167 3232 4885 6826 7468 8411
Size: 1183 888 1031 65 1653 1941 642 943
Cuts at: 8411 8568 8879 10157 10331 65
Size: 157 311 1278 174 1396
Fragments arranged by size:
1941 1653 1396 1278 1183 1031 943 888
642 311 174 157 65
BsrFI r'CCGG_y
Cuts at: 1700 1810 2583 8301 10306 10493 1700
Size: 110 773 5718 2005 187 2869
Fragments arranged by size:
5718 2869 2005 773 187 110
BsrGI T'GTAC_A
Cuts at: 96 2308 4800 5868 96
Size: 2212 2492 1068 5890
Fragments arranged by size:
5890 2492 2212 1068
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11662
BssSI C'ACGA_G
Cuts at: 9722 10734 9722
Size: 1012 10650
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2509 794
Size: 1715 9947
BstBI TT'CG_AA
Cuts at: 2679 10466 2679
Size: 7787 3875
BstEII G'GTnAC_C
Cuts at: 1184 8377 1184
Size: 7193 4469
BstYI r'GATC_y
Cuts at: 1972 2053 3292 3305 5169 5813 6016 6490 6826
Size: 81 1239 13 1864 644 203 474 336
Cuts at: 6826 6904 7134 7666 8992 9359 9744 9761 11213
Size: 78 230 532 1326 367 385 17 1452
Cuts at: 11213 11299 11311 11657 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1864 1452 1326 1239 644 532 474
385 367 346 336 230 203 86 81
78 17 13 12
BstZ17I GTA'TAC
Cuts at: 4815 4815
Size: 11662
Bsu36I CC'TnA_GG
Cuts at: 2036 2036
Size: 11662
BtgI C'CryG_G
Cuts at: 513 1065 2058 2079 4398 5806 513
Size: 552 993 21 2319 1408 6369
Fragments arranged by size:
6369 2319 1408 993 552 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 7209 487
Size: 683 56 5983 4940
Fragments arranged by size:
5983 4940 683 56
BtsI GCAGTG_nn'
Cuts at: 2448 2869 3594 3754 3899 6094 6165 6769 6874
Size: 421 725 160 145 2195 71 604 105
Cuts at: 6874 7090 7510 7926 9178 9972 9992 2448
Size: 216 420 416 1252 794 20 4118
Fragments arranged by size:
4118 2195 1252 794 725 604 421 420
416 216 160 145 105 71 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11627
DraI TTT'AAA
Cuts at: 6333 6754 8966 6333
Size: 421 2212 9029
Fragments arranged by size:
9029 2212 421
DraIII CAC_nnn'GTG
Cuts at: 2443 3388 2443
Size: 945 10717
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10669 809
Size: 507 9353 1802
Fragments arranged by size:
9353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 7012 9109
Size: 54 1425 50 275 18 252 4930 2097
Cuts at: 9109 9998 11627 8
Size: 889 1629 43
Fragments arranged by size:
4930 2097 1629 1425 889 275 252 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9109 1537
Size: 275 7297 4090
Fragments arranged by size:
7297 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2562 2946 3507 4126 4833 5992 6984
Size: 765 444 384 561 619 707 1159 992
Cuts at: 6984 7296 8388 9592 1353
Size: 312 1092 1204 3423
Fragments arranged by size:
3423 1204 1159 1092 992 765 707 619
561 444 384 312
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 10249 10623 10769 387
Size: 1064 138 8660 374 146 1280
Fragments arranged by size:
8660 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3582 4915 7093 3582
Size: 1333 2178 8151
Fragments arranged by size:
8151 2178 1333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2586 2973 4481 4595 5273 6705 6758
Size: 393 954 387 1508 114 678 1432 53
Cuts at: 6758 7258 7317 7318 8802 9725 10322 11109 11377
Size: 500 59 1 1484 923 597 787 268
Cuts at: 11377 1239
Size: 1524
Fragments arranged by size:
1524 1508 1484 1432 954 923 787 678
597 500 393 387 268 114 59 53
1
EcoNI CCTnn'n_nnAGG
Cuts at: 2248 7420 2248
Size: 5172 6490
EcoO109I rG'GnC_Cy
Cuts at: 2047 4149 5776 6168 2047
Size: 2102 1627 392 7541
Fragments arranged by size:
7541 2102 1627 392
EcoRI G'AATT_C
Cuts at: 4027 4373 6079 7597 8971 9349 4027
Size: 346 1706 1518 1374 378 6340
Fragments arranged by size:
6340 1706 1518 1374 378 346
EcoRV GAT'ATC
Cuts at: 6973 6973
Size: 11662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3297 6804 7021 8936 3297
Size: 3507 217 1915 6023
Fragments arranged by size:
6023 3507 1915 217
FspI TGC'GCA
Cuts at: 6052 10168 6052
Size: 4116 7546
HaeII r_GCGC'y
Cuts at: 1678 10809 1678
Size: 9131 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2720 2752 2834
Size: 32 135 32 557 32 559 32 82
Cuts at: 2834 2866 2870 2902 3975 4007 4103 4135 4163
Size: 32 4 32 1073 32 96 32 28
Cuts at: 4163 4195 4208 4240 4585 4617 4772 4804 4925
Size: 32 13 32 345 32 155 32 121
Cuts at: 4925 4957 5154 5186 5204 5236 5452 5484 6509
Size: 32 197 32 18 32 216 32 1025
Cuts at: 6509 6541 6732 6764 7019 7051 7079 7111 7256
Size: 32 191 32 255 32 28 32 145
Cuts at: 7256 7288 7316 7348 7501 7533 10303 10335 10377
Size: 32 28 32 153 32 2770 32 42
Cuts at: 10377 10409 1373
Size: 32 2626
Fragments arranged by size:
2770 2626 1073 1025 559 557 345 255
216 197 191 155 153 145 135 121
96 82 42 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 28 28 28 18
13 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 2419 6223 6804 9006 9252
Size: 988 281 72 409 3804 581 2202 246
Cuts at: 9252 669
Size: 3079
Fragments arranged by size:
3804 3079 2202 988 581 409 281 246
72
HindIII A'AGCT_T
Cuts at: 748 2556 3959 5067 5753 6266 8747 748
Size: 1808 1403 1108 686 513 2481 3663
Fragments arranged by size:
3663 2481 1808 1403 1108 686 513
HpaI GTT'AAC
Cuts at: 2010 6223 9252 2010
Size: 4213 3029 4420
Fragments arranged by size:
4420 4213 3029
KpnI G_GTAC'C
Cuts at: 8987 8987
Size: 11662
MfeI C'AATT_G
Cuts at: 3704 9261 3704
Size: 5557 6105
MluI A'CGCG_T
Cuts at: 11538 11538
Size: 11662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3668 3924 4073 5755 6304 8268 8656 10776
Size: 2363 256 149 1682 549 1964 388 2120
Cuts at: 10776 10960 1305
Size: 184 2007
Fragments arranged by size:
2363 2120 2007 1964 1682 549 388 256
184 149
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2084 7014 10
Size: 54 1425 343 252 4930 4658
Fragments arranged by size:
4930 4658 1425 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 2090 2304 3066 5537 5942 6659
Size: 648 852 72 214 762 2471 405 717
Cuts at: 6659 7376 7611 8198 8361 9620 9979 10138 518
Size: 717 235 587 163 1259 359 159 2042
Fragments arranged by size:
2471 2042 1259 852 762 717 717 648
587 405 359 235 214 163 159 72
MspA1I CmG'CkG
Cuts at: 1606 4644 5251 5497 7058 8314 8618 9078 9755
Size: 3038 607 246 1561 1256 304 460 677
Cuts at: 9755 10903 11148 1606
Size: 1148 245 2120
Fragments arranged by size:
3038 2120 1561 1256 1148 677 607 460
304 246 245
NaeI GCC'GGC
Cuts at: 2585 2585
Size: 11662
NcoI C'CATG_G
Cuts at: 513 1065 2058 2079 4398 5806 513
Size: 552 993 21 2319 1408 6369
Fragments arranged by size:
6369 2319 1408 993 552 21
NdeI CA'TA_TG
Cuts at: 387 4334 7672 387
Size: 3947 3338 4377
Fragments arranged by size:
4377 3947 3338
NgoMIV G'CCGG_C
Cuts at: 2583 2583
Size: 11662
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11662
NotI GC'GGCC_GC
Cuts at: 9109 9109
Size: 11662
NsiI A_TGCA'T
Cuts at: 7153 8958 7153
Size: 1805 9857
NspI r_CATG'y
Cuts at: 1132 1229 2303 4585 5911 6057 6485 6664 8586
Size: 97 1074 2282 1326 146 428 179 1922
Cuts at: 8586 9020 10565 1132
Size: 434 1545 2229
Fragments arranged by size:
2282 2229 1922 1545 1326 1074 434 428
179 146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 11662
PciI A'CATG_T
Cuts at: 10561 10561
Size: 11662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 11662
PfoI T'CCnGG_A
Cuts at: 5172 7137 5172
Size: 1965 9697
PmeI GTTT'AAAC
Cuts at: 8966 8966
Size: 11662
PmlI CAC'GTG
Cuts at: 5210 5210
Size: 11662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9731 9763 11244 11276 9731
Size: 32 1481 32 10117
Fragments arranged by size:
10117 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 5776 6168 2047
Size: 3729 392 7541
Fragments arranged by size:
7541 3729 392
PshAI GACnn'nnGTC
Cuts at: 1613 2694 1613
Size: 1081 10581
PsiI TTA'TAA
Cuts at: 3141 9232 3141
Size: 6091 5571
PspOMI G'GGCC_C
Cuts at: 4148 4148
Size: 11662
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2301 2333 5332 5364 2301
Size: 32 2999 32 8599
Fragments arranged by size:
8599 2999 32 32
PstI C_TGCA'G
Cuts at: 830 2514 2947 4620 8545 9014 830
Size: 1684 433 1673 3925 469 3478
Fragments arranged by size:
3925 3478 1684 1673 469 433
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11662
PvuII CAG'CTG
Cuts at: 4644 5251 5497 8314 8618 4644
Size: 607 246 2817 304 7688
Fragments arranged by size:
7688 2817 607 304 246
SacI G_AGCT'C
Cuts at: 3584 4917 7095 3584
Size: 1333 2178 8151
Fragments arranged by size:
8151 2178 1333
SalI G'TCGA_C
Cuts at: 1936 9004 1936
Size: 7068 4594
SanDI GG'GwC_CC
Cuts at: 6168 6168
Size: 11662
SapI GCTCTTCn'nnn_
Cuts at: 2946 7296 2946
Size: 4350 7312
SbfI CC_TGCA'GG
Cuts at: 9014 9014
Size: 11662
ScaI AGT'ACT
Cuts at: 9910 11622 9910
Size: 1712 9950
SexAI A'CCwGG_T
Cuts at: 5862 7976 8779 5862
Size: 2114 803 8745
Fragments arranged by size:
8745 2114 803
SfcI C'TryA_G
Cuts at: 826 1045 2510 2692 2776 2943 4407 4616 6549
Size: 219 1465 182 84 167 1464 209 1933
Cuts at: 6549 8541 9010 10145 10826 11017 826
Size: 1992 469 1135 681 191 1471
Fragments arranged by size:
1992 1933 1471 1465 1464 1135 681 469
219 209 191 182 167 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11662
SmaI CCC'GGG
Cuts at: 1872 8989 1872
Size: 7117 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2889 3665 3914 4471 4508 4544
Size: 197 926 946 776 249 557 37 36
Cuts at: 4544 4793 9766 10667 10929 820
Size: 249 4973 901 262 1553
Fragments arranged by size:
4973 1553 946 926 901 776 557 262
249 249 197 37 36
SnaBI TAC'GTA
Cuts at: 493 9453 493
Size: 8960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11662
SphI G_CATG'C
Cuts at: 1132 6664 9020 1132
Size: 5532 2356 3774
Fragments arranged by size:
5532 3774 2356
SspI AAT'ATT
Cuts at: 5 52 4000 5833 7537 7699 9586 5
Size: 47 3948 1833 1704 162 1887 2081
Fragments arranged by size:
3948 2081 1887 1833 1704 162 47
StuI AGG'CCT
Cuts at: 1517 3872 8477 1517
Size: 2355 4605 4702
Fragments arranged by size:
4702 4605 2355
StyI C'CwwG_G
Cuts at: 513 1065 2058 2079 3618 4398 4731 5158 5806
Size: 552 993 21 1539 780 333 427 648
Cuts at: 5806 6424 9101 513
Size: 618 2677 3074
Fragments arranged by size:
3074 2677 1539 993 780 648 618 552
427 333 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2092 2113 9689 9706 9859 10044 10489
Size: 1255 512 21 7576 17 153 185 445
Cuts at: 10489 325
Size: 1498
Fragments arranged by size:
7576 1498 1255 512 445 185 153 21
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2308 4800 5868
Size: 274 80 33 51 1443 331 2492 1068
Cuts at: 5868 8170 9908 11620 96
Size: 2302 1738 1712 138
Fragments arranged by size:
2492 2302 1738 1712 1443 1068 331 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2764 2796 7615 7647 8670 8702 11622 11654 2764
Size: 32 4819 32 1023 32 2920 32 2772
Fragments arranged by size:
4819 2920 2772 1023 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11662
XbaI T'CTAG_A
Cuts at: 8998 8998
Size: 11662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3316 6298 8708 3316
Size: 2982 2410 6270
Fragments arranged by size:
6270 2982 2410
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11662
XmaI C'CCGG_G
Cuts at: 1870 8987 1870
Size: 7117 4545
XmnI GAAnn'nnTTC
Cuts at: 4377 6784 6964 7707 7952 8919 9791 4377
Size: 2407 180 743 245 967 872 6248
Fragments arranged by size:
6248 2407 967 872 743 245 180
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5405 9470 276
Size: 53 83 186 936 3871 4065 2468
Fragments arranged by size:
4065 3871 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BsiWI BsmBI
BstXI FseI FspAI KasI NarI NruI PacI PspXI
RsrII SacII SfiI SfoI SrfI SwaI