Restriction map for
pFN21AA0310
(Circular) (Six-base) MAPSORT of: pFN21AA0310.seq Check: 8507 from: 1 to: 11776
pFN21AA0310 11776 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4918 6760 7639 7650 4918
Size: 1842 879 11 9044
Fragments arranged by size:
9044 1842 879 11
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7158 8592 9586 278
Size: 53 83 186 936 5622 1434 994 2468
Fragments arranged by size:
5622 2468 1434 994 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2179 5322 5577 7038 9119 1937
Size: 242 3143 255 1461 2081 4594
Fragments arranged by size:
4594 3143 2081 1461 255 242
Acc65I G'GTAC_C
Cuts at: 9097 9097
Size: 11776
AclI AA'CG_TT
Cuts at: 1563 9075 9903 10276 1563
Size: 7512 828 373 3063
Fragments arranged by size:
7512 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2379 2475 2724 3093 3663 4113 4397 5742
Size: 747 96 249 369 570 450 284 1345
Cuts at: 5742 6393 9839 11223 11491 1632
Size: 651 3446 1384 268 1917
Fragments arranged by size:
3446 1917 1384 1345 747 651 570 450
369 284 268 249 96
AflII C'TTAA_G
Cuts at: 820 1017 4996 820
Size: 197 3979 7600
Fragments arranged by size:
7600 3979 197
AflIII A'CryG_T
Cuts at: 1203 3253 5025 6465 8544 10675 11652 1203
Size: 2050 1772 1440 2079 2131 977 1327
Fragments arranged by size:
2131 2079 2050 1772 1440 1327 977
AgeI A'CCGG_T
Cuts at: 3841 10607 3841
Size: 6766 5010
AhdI GACnn_n'nnGTC
Cuts at: 3565 4610 6598 7159 10505 3565
Size: 1045 1988 561 3346 4836
Fragments arranged by size:
4836 3346 1988 1045 561
AleI CACnn'nnGTG
Cuts at: 1166 3344 4892 4925 5835 7479 7627 1166
Size: 2178 1548 33 910 1644 148 5315
Fragments arranged by size:
5315 2178 1644 1548 910 148 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3362 4943 5088 9028 1184
Size: 2178 1581 145 3940 3932
Fragments arranged by size:
3940 3932 2178 1581 145
AlwNI CAG_nnn'CTG
Cuts at: 2453 2467 3273 3628 5185 5338 5438 5470 7378
Size: 14 806 355 1557 153 100 32 1908
Cuts at: 7378 7976 8780 9519 11091 2453
Size: 598 804 739 1572 3138
Fragments arranged by size:
3138 1908 1572 1557 806 804 739 598
355 153 100 32 14
ApaI G_GGCC'C
Cuts at: 2783 3477 4149 7941 8963 2783
Size: 694 672 3792 1022 5596
Fragments arranged by size:
5596 3792 1022 694 672
ApaLI G'TGCA_C
Cuts at: 3551 4014 4061 9833 10989 3551
Size: 463 47 5772 1156 4338
Fragments arranged by size:
5772 4338 1156 463 47
ApoI r'AATT_y
Cuts at: 1493 3392 6335 6619 8137 8150 8428 9085 9311
Size: 1899 2943 284 1518 13 278 657 226
Cuts at: 9311 9463 11572 1493
Size: 152 2109 1697
Fragments arranged by size:
2943 2109 1899 1697 1518 657 284 278
226 152 13
AseI AT'TA_AT
Cuts at: 160 1032 6540 10330 160
Size: 872 5508 3790 1606
Fragments arranged by size:
5508 3790 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11776
AvaI C'yCGr_G
Cuts at: 1870 1943 2677 3018 3451 3665 3784 4115 5651
Size: 73 734 341 433 214 119 331 1536
Cuts at: 5651 5657 5673 5936 6496 6845 6981 7831 7837
Size: 6 16 263 560 349 136 850 6
Cuts at: 7837 7933 8170 8221 9101 1870
Size: 96 237 51 880 4545
Fragments arranged by size:
4545 1536 880 850 734 560 433 349
341 331 263 237 214 136 119 96
73 51 16 6 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5719 5752 5719
Size: 33 11743
BamHI G'GATC_C
Cuts at: 2161 2500 7295 9106 9473 2161
Size: 339 4795 1811 367 4464
Fragments arranged by size:
4795 4464 1811 367 339
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2725 3346 4084 4919 4927
Size: 325 217 627 938 621 738 835 8
Cuts at: 4927 5104 5188 6221 7382 7807 8098 8179 9097
Size: 177 84 1033 1161 425 291 81 918
Cuts at: 9097 10552 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1161 1033 938 918 835 738
627 621 425 325 291 217 177 84
81 8
BanII G_rGCy'C
Cuts at: 1391 2331 2550 2783 2955 3118 3429 3477 3529
Size: 940 219 233 172 163 311 48 52
Cuts at: 3529 3791 4149 4166 4627 4984 5176 5658 7702
Size: 262 358 17 461 357 192 482 2044
Cuts at: 7702 7780 7823 7838 7941 7998 8072 8536 8578
Size: 78 43 15 103 57 74 464 42
Cuts at: 8578 8793 8963 9032 9095 1391
Size: 215 170 69 63 4072
Fragments arranged by size:
4072 2044 940 482 464 461 358 357
311 262 233 219 215 192 172 170
163 103 78 74 69 63 57 52
48 43 42 17 15
BbeI G_GCGC'C
Cuts at: 5108 8102 5108
Size: 2994 8782
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4974 6602 928
Size: 971 3075 1628 6102
Fragments arranged by size:
6102 3075 1628 971
BbvCI CC'TCA_GC
Cuts at: 2619 4263 6113 7858 2619
Size: 1644 1850 1745 6537
Fragments arranged by size:
6537 1850 1745 1644
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5753 5787 8161 8195 8930
Size: 34 50 34 3732 34 2374 34 735
Cuts at: 8930 8964 9967 10001 1903
Size: 34 1003 34 3678
Fragments arranged by size:
3732 3678 2374 1003 735 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4090 4116 5720 9668 10878 4090
Size: 26 1604 3948 1210 4988
Fragments arranged by size:
4988 3948 1604 1210 26
BclI T'GATC_A
Cuts at: 1548 4504 7536 1548
Size: 2956 3032 5788
Fragments arranged by size:
5788 3032 2956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2680 4353 5160
Size: 107 122 71 631 605 1008 1673 807
Cuts at: 5160 5347 5662 7936 8002 10387 136
Size: 187 315 2274 66 2385 1525
Fragments arranged by size:
2385 2274 1673 1525 1008 807 631 605
315 187 122 107 71 66
BglII A'GATC_T
Cuts at: 11771 11771
Size: 11776
BlpI GC'TnA_GC
Cuts at: 3610 4497 4524 8126 8997 9193 3610
Size: 887 27 3602 871 196 6193
Fragments arranged by size:
6193 3602 887 871 196 27
Bme1580I G_kGCm'C
Cuts at: 1790 2730 2783 3085 3477 3498 3555 4018 4065
Size: 940 53 302 392 21 57 463 47
Cuts at: 4065 4087 4149 7941 8963 9837 10993 1790
Size: 22 62 3792 1022 874 1156 2573
Fragments arranged by size:
3792 2573 1156 1022 940 874 463 392
302 62 57 53 47 22 21
BmgBI CAC'GTC
Cuts at: 1318 6468 1318
Size: 5150 6626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2213 2436 2826
Size: 416 53 83 399 24 796 223 390
Cuts at: 2826 2947 3065 5827 7366 7558 7913 9507 10465
Size: 121 118 2762 1539 192 355 1594 958
Cuts at: 10465 442
Size: 1753
Fragments arranged by size:
2762 1753 1594 1539 958 796 416 399
390 355 223 192 121 118 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2088 1056
Size: 1032 10744
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 11776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 3202 4001 4269 4467 4600 5770 5977
Size: 798 1165 799 268 198 133 1170 207
Cuts at: 5977 6174 7698 7964 8012 8039 8550 8827 10436
Size: 197 1524 266 48 27 511 277 1609
Cuts at: 10436 1239
Size: 2579
Fragments arranged by size:
2579 1609 1524 1170 1165 799 798 511
277 268 266 207 198 197 133 48
27
Bpu10I CC'TnA_GC
Cuts at: 2561 2619 2954 4263 4685 6113 7824 7858 2561
Size: 58 335 1309 422 1428 1711 34 6479
Fragments arranged by size:
6479 1711 1428 1309 422 335 58 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4397 9016 9901 10766 11064 4397
Size: 4619 885 865 298 5109
Fragments arranged by size:
5109 4619 885 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3728 3908 3998 6006 6684 6848 7382
Size: 617 2229 180 90 2008 678 164 534
Cuts at: 7382 8266 10439 882
Size: 884 2173 2219
Fragments arranged by size:
2229 2219 2173 2008 884 678 617 534
180 164 90
BsaAI yAC'GTr
Cuts at: 493 1204 3256 7077 7629 8906 9567 493
Size: 711 2052 3821 552 1277 661 2702
Fragments arranged by size:
3821 2702 2052 1277 711 661 552
BsaBI GATnn'nnATC
Cuts at: 11770 11770
Size: 11776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5105 7155 8099 8589
Size: 53 83 186 936 3572 2050 944 490
Cuts at: 8589 9583 9965 10600 275
Size: 994 382 635 1451
Fragments arranged by size:
3572 2050 1451 994 944 936 635 490
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3521 3841 5539 5868 7052 10209 10607 10881 11028
Size: 320 1698 329 1184 3157 398 274 147
Cuts at: 11028 11439 3521
Size: 411 3858
Fragments arranged by size:
3858 3157 1698 1184 411 398 329 320
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2172 2202 3431 3461 4200
Size: 30 165 30 769 30 1229 30 739
Cuts at: 4200 4230 6668 6698 8037 8067 8523 8553 1178
Size: 30 2438 30 1339 30 456 30 4401
Fragments arranged by size:
4401 2438 1339 1229 769 739 456 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3170 4415 4418 5343 5677 6494 6794 6797
Size: 1984 1245 3 925 334 817 300 3
Cuts at: 6797 7622 8462 8830 8880 1186
Size: 825 840 368 50 4082
Fragments arranged by size:
4082 1984 1245 925 840 825 817 368
334 300 50 3 3
BseYI C'CCAG_C
Cuts at: 2042 2245 2596 2717 2818 3062 3296 3771 4165
Size: 203 351 121 101 244 234 475 394
Cuts at: 4165 4794 5175 5196 5666 6798 7584 8610 8966
Size: 629 381 21 470 1132 786 1026 356
Cuts at: 8966 10979 11475 2042
Size: 2013 496 2343
Fragments arranged by size:
2343 2013 1132 1026 786 629 496 475
470 394 381 356 351 244 234 203
121 101 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2448 2678 3958 4361 4896 5424 6059
Size: 573 712 230 1280 403 535 528 635
Cuts at: 6059 7571 7797 7962 1163
Size: 1512 226 165 4977
Fragments arranged by size:
4977 1512 1280 712 635 573 535 528
403 230 226 165
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2554 9226 9987 11015 1540
Size: 275 186 553 6672 761 1028 2301
Fragments arranged by size:
6672 2301 1028 761 553 275 186
BsiHKAI G_wGCw'C
Cuts at: 2362 2955 2985 3118 3341 3555 4018 4065 4855
Size: 593 30 133 223 214 463 47 790
Cuts at: 4855 5916 6060 7830 8578 9032 9837 9922 10993
Size: 1061 144 1770 748 454 805 85 1071
Cuts at: 10993 2362
Size: 3145
Fragments arranged by size:
3145 1770 1071 1061 805 790 748 593
463 454 223 214 144 133 85 47
30
BsiWI C'GTAC_G
Cuts at: 3041 3041
Size: 11776
BsmI GAATG_Cn'
Cuts at: 9287 9380 9287
Size: 93 11683
BsmBI CGTCTCn'nnnn_
Cuts at: 2095 2517 2838 7080 2095
Size: 422 321 4242 6791
Fragments arranged by size:
6791 4242 422 321
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2331 2362 2550 2730 2783 2955 2985
Size: 399 541 31 188 180 53 172 30
Cuts at: 2985 3085 3118 3341 3429 3477 3498 3529 3555
Size: 100 33 223 88 48 21 31 26
Cuts at: 3555 3791 4018 4065 4087 4149 4166 4627 4855
Size: 236 227 47 22 62 17 461 228
Cuts at: 4855 4984 5176 5658 5916 6060 7702 7780 7823
Size: 129 192 482 258 144 1642 78 43
Cuts at: 7823 7830 7838 7941 7998 8072 8536 8578 8793
Size: 7 8 103 57 74 464 42 215
Cuts at: 8793 8963 9032 9095 9837 9922 10993 1391
Size: 170 69 63 742 85 1071 2174
Fragments arranged by size:
2174 1642 1071 742 541 482 464 461
399 258 236 228 227 223 215 192
188 180 172 170 144 129 103 100
88 85 78 74 69 63 62 57
53 48 47 43 42 33 31 31
30 26 22 21 17 8 7
BspEI T'CCGG_A
Cuts at: 7052 11439 7052
Size: 4387 7389
BspHI T'CATG_A
Cuts at: 2788 4561 6987 9665 11395 2788
Size: 1773 2426 2678 1730 3169
Fragments arranged by size:
3169 2678 2426 1773 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4615 4918 5122 6760 7400 7639 7650 9131
Size: 3771 303 204 1638 640 239 11 1481
Cuts at: 9131 844
Size: 3489
Fragments arranged by size:
3771 3489 1638 1481 640 303 239 204
11
BsrBI CCG'CTC
Cuts at: 1394 1823 5662 7657 8258 9663 1394
Size: 429 3839 1995 601 1405 3507
Fragments arranged by size:
3839 3507 1995 1405 601 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3307 6669 6921 10271 10445 65
Size: 1183 2059 3362 252 3350 174 1396
Fragments arranged by size:
3362 3350 2059 1396 1183 252 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2152 2985 3841 5490 6036 6164 6421
Size: 110 342 833 856 1649 546 128 257
Cuts at: 6421 8623 10420 10607 1700
Size: 2202 1797 187 2869
Fragments arranged by size:
2869 2202 1797 1649 856 833 546 342
257 187 128 110
BsrGI T'GTAC_A
Cuts at: 96 5028 96
Size: 4932 6844
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11776
BssSI C'ACGA_G
Cuts at: 9836 10848 9836
Size: 1012 10764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3273 3550 4850 9067 794
Size: 2479 277 1300 4217 3503
Fragments arranged by size:
4217 3503 2479 1300 277
BstBI TT'CG_AA
Cuts at: 3837 10580 3837
Size: 6743 5033
BstEII G'GTnAC_C
Cuts at: 1184 2590 1184
Size: 1406 10370
BstXI CCAn_nnnn'nTGG
Cuts at: 2531 2646 5817 7171 7633 2531
Size: 115 3171 1354 462 6674
Fragments arranged by size:
6674 3171 1354 462 115
BstYI r'GATC_y
Cuts at: 1972 2161 2500 5806 7295 9106 9473 9858 9875
Size: 189 339 3306 1489 1811 367 385 17
Cuts at: 9875 11327 11413 11425 11771 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
3306 1977 1811 1489 1452 385 367 346
339 189 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5463 8850 5463
Size: 3387 8389
BtgI C'CryG_G
Cuts at: 513 1065 3592 3757 4050 5420 6297 6654 7122
Size: 552 2527 165 293 1370 877 357 468
Cuts at: 7122 7179 513
Size: 57 5110
Fragments arranged by size:
5110 2527 1370 877 552 468 357 293
165 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3137 4310 7474 487
Size: 683 56 1911 1173 3164 4789
Fragments arranged by size:
4789 3164 1911 1173 683 56
BtsI GCAGTG_nn'
Cuts at: 2130 3539 3941 4711 6250 6768 7718 7925 9292
Size: 1409 402 770 1539 518 950 207 1367
Cuts at: 9292 10086 10106 2130
Size: 794 20 3800
Fragments arranged by size:
3800 1539 1409 1367 950 794 770 518
402 207 20
ClaI AT'CG_AT
Cuts at: 1554 3702 8393 1554
Size: 2148 4691 4937
Fragments arranged by size:
4937 4691 2148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2217 2252 529
Size: 35 1653 35 10053
Fragments arranged by size:
10053 1653 35 35
DraI TTT'AAA
Cuts at: 8433 9080 8433
Size: 647 11129
DraIII CAC_nnn'GTG
Cuts at: 6157 8021 9067 6157
Size: 1864 1046 8866
Fragments arranged by size:
8866 1864 1046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2029 2588 10783 809
Size: 507 713 559 8195 1802
Fragments arranged by size:
8195 1802 713 559 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2880 5392 5423
Size: 54 1425 50 275 18 1050 2512 31
Cuts at: 5423 5472 5696 6093 6507 6886 7458 7617 7890
Size: 49 224 397 414 379 572 159 273
Cuts at: 7890 8766 9049 9223 10112 11741 8
Size: 876 283 174 889 1629 43
Fragments arranged by size:
2512 1629 1425 1050 889 876 572 414
397 379 283 275 273 224 174 159
54 50 49 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 9223 1537
Size: 275 7411 4090
Fragments arranged by size:
7411 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2836 4115 4284 4860 6021 6771 7490 7580
Size: 1483 1279 169 576 1161 750 719 90
Cuts at: 7580 7599 8439 8551 9706 1353
Size: 19 840 112 1155 3423
Fragments arranged by size:
3423 1483 1279 1161 1155 840 750 719
576 169 112 90 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3228 3914 7460 10363 10737 10883
Size: 1064 138 1639 686 3546 2903 374 146
Cuts at: 10883 387
Size: 1280
Fragments arranged by size:
3546 2903 1639 1280 1064 686 374 146
138
EcoICRI GAG'CTC
Cuts at: 2953 3116 8576 9030 2953
Size: 163 5460 454 5699
Fragments arranged by size:
5699 5460 454 163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2379 2475 2724 3093 3202 3663
Size: 393 405 342 96 249 369 109 461
Cuts at: 3663 4001 4113 4269 4397 4467 4600 5742 5770
Size: 338 112 156 128 70 133 1142 28
Cuts at: 5770 5977 6174 6393 7698 7964 8012 8039 8550
Size: 207 197 219 1305 266 48 27 511
Cuts at: 8550 8827 9839 10436 11223 11491 1239
Size: 277 1012 597 787 268 1524
Fragments arranged by size:
1524 1305 1142 1012 787 597 511 461
405 393 369 342 338 277 268 266
249 219 207 197 156 133 128 112
109 96 70 48 28 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2774 3228 3275 4999 6411 7646 8842 8952 2774
Size: 454 47 1724 1412 1235 1196 110 5598
Fragments arranged by size:
5598 1724 1412 1235 1196 454 110 47
EcoO109I rG'GnC_Cy
Cuts at: 2318 2708 2779 2780 3280 3293 3474 3618 4137
Size: 390 71 1 500 13 181 144 519
Cuts at: 4137 4145 4408 5467 5604 5851 6178 6500 7204
Size: 8 263 1059 137 247 327 322 704
Cuts at: 7204 7937 7938 7952 7973 8194 8225 8522 8607
Size: 733 1 14 21 221 31 297 85
Cuts at: 8607 8744 8761 8959 8960 2318
Size: 137 17 198 1 5134
Fragments arranged by size:
5134 1059 733 704 519 500 390 327
322 297 263 247 221 198 181 144
137 137 85 71 31 21 17 14
13 8 1 1 1
EcoRI G'AATT_C
Cuts at: 9085 9463 9085
Size: 378 11398
EcoRV GAT'ATC
Cuts at: 5717 5717
Size: 11776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5022 6339 7303 5022
Size: 1317 964 9495
Fragments arranged by size:
9495 1317 964
FspI TGC'GCA
Cuts at: 10282 10282
Size: 11776
HaeII r_GCGC'y
Cuts at: 1678 2189 4244 5108 8102 10923 1678
Size: 511 2055 864 2994 2821 2531
Fragments arranged by size:
2994 2821 2531 2055 864 511
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2172 2204 3200 3232 3431
Size: 32 135 32 600 32 996 32 199
Cuts at: 3431 3463 3551 3583 4200 4232 4283 4315 4596
Size: 32 88 32 617 32 51 32 281
Cuts at: 4596 4628 5950 5982 6385 6417 6584 6616 6770
Size: 32 1322 32 403 32 167 32 154
Cuts at: 6770 6802 7145 7177 8037 8069 10417 10449 10491
Size: 32 343 32 860 32 2348 32 42
Cuts at: 10491 10523 1373
Size: 32 2626
Fragments arranged by size:
2626 2348 1322 996 860 617 600 403
343 281 199 167 154 135 88 51
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5323 8587 9120 9366 669
Size: 988 281 3385 3264 533 246 3079
Fragments arranged by size:
3385 3264 3079 988 533 281 246
HindIII A'AGCT_T
Cuts at: 748 3675 7280 8364 748
Size: 2927 3605 1084 4160
Fragments arranged by size:
4160 3605 2927 1084
HpaI GTT'AAC
Cuts at: 9366 9366
Size: 11776
KasI G'GCGC_C
Cuts at: 5104 8098 5104
Size: 2994 8782
KpnI G_GTAC'C
Cuts at: 9101 9101
Size: 11776
MfeI C'AATT_G
Cuts at: 9375 9375
Size: 11776
MluI A'CGCG_T
Cuts at: 11652 11652
Size: 11776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2156 3637 4694 7158 7728 8086 8498 10890
Size: 851 1481 1057 2464 570 358 412 2392
Cuts at: 10890 11074 1305
Size: 184 2007
Fragments arranged by size:
2464 2392 2007 1481 1057 851 570 412
358 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2882 5425 6095 6509 7460
Size: 54 1425 343 1050 2543 670 414 951
Cuts at: 7460 7892 8768 9051 10
Size: 432 876 283 2735
Fragments arranged by size:
2735 2543 1425 1050 951 876 670 432
414 343 283 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2295 2529 2583 3344 4892 4925 5835
Size: 648 1129 234 54 761 1548 33 910
Cuts at: 5835 6354 7479 7627 7692 9734 10093 10252 518
Size: 519 1125 148 65 2042 359 159 2042
Fragments arranged by size:
2042 2042 1548 1129 1125 910 761 648
519 359 234 159 148 65 54 33
MspA1I CmG'CkG
Cuts at: 1606 2721 3360 3491 3947 5338 5402 7588 8614
Size: 1115 639 131 456 1391 64 2186 1026
Cuts at: 8614 8970 9001 9192 9869 11017 11262 1606
Size: 356 31 191 677 1148 245 2120
Fragments arranged by size:
2186 2120 1391 1148 1115 1026 677 639
456 356 245 191 131 64 31
NaeI GCC'GGC
Cuts at: 6423 8625 6423
Size: 2202 9574
NarI GG'CG_CC
Cuts at: 5105 8099 5105
Size: 2994 8782
NcoI C'CATG_G
Cuts at: 513 1065 6297 7122 7179 513
Size: 552 5232 825 57 5110
Fragments arranged by size:
5232 5110 825 552 57
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11776
NgoMIV G'CCGG_C
Cuts at: 6421 8623 6421
Size: 2202 9574
NheI G'CTAG_C
Cuts at: 1052 2084 1052
Size: 1032 10744
NotI GC'GGCC_GC
Cuts at: 9223 9223
Size: 11776
NruI TCG'CGA
Cuts at: 7493 7493
Size: 11776
NspI r_CATG'y
Cuts at: 1132 1229 2300 2366 2542 4069 8548 9134 10679
Size: 97 1071 66 176 1527 4479 586 1545
Cuts at: 10679 1132
Size: 2229
Fragments arranged by size:
4479 2229 1545 1527 1071 586 176 97
66
PasI CC'CwG_GG
Cuts at: 1348 3224 4141 5149 7378 7780 7970 8078 8201
Size: 1876 917 1008 2229 402 190 108 123
Cuts at: 8201 8519 8860 1348
Size: 318 341 4264
Fragments arranged by size:
4264 2229 1876 1008 917 402 341 318
190 123 108
PciI A'CATG_T
Cuts at: 8544 10675 8544
Size: 2131 9645
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3055 3628 4205 6157 6266 6445 6529 7378
Size: 1947 573 577 1952 109 179 84 849
Cuts at: 7378 1108
Size: 5506
Fragments arranged by size:
5506 1952 1947 849 577 573 179 109
84
PfoI T'CCnGG_A
Cuts at: 2491 2965 4577 4858 7163 7989 8055 8527 9023
Size: 474 1612 281 2305 826 66 472 496
Cuts at: 9023 2491
Size: 5244
Fragments arranged by size:
5244 2305 1612 826 496 474 472 281
66
PmeI GTTT'AAAC
Cuts at: 9080 9080
Size: 11776
PmlI CAC'GTG
Cuts at: 3256 7077 7629 8906 3256
Size: 3821 552 1277 6126
Fragments arranged by size:
6126 3821 1277 552
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4310 4342 8534 8560 8566 8592 9845 9877 11358
Size: 32 4192 26 6 26 1253 32 1481
Cuts at: 11358 11390 4310
Size: 32 4696
Fragments arranged by size:
4696 4192 1481 1253 32 32 32 26
26 6
PpuMI rG'GwC_Cy
Cuts at: 2318 2708 4137 5467 5851 6500 7952 8225 8607
Size: 390 1429 1330 384 649 1452 273 382
Cuts at: 8607 2318
Size: 5487
Fragments arranged by size:
5487 1452 1429 1330 649 390 384 382
273
PshAI GACnn'nnGTC
Cuts at: 1613 3920 5328 8223 1613
Size: 2307 1408 2895 5166
Fragments arranged by size:
5166 2895 2307 1408
PsiI TTA'TAA
Cuts at: 9346 9346
Size: 11776
PspOMI G'GGCC_C
Cuts at: 2779 3473 4145 7937 8959 2779
Size: 694 672 3792 1022 5596
Fragments arranged by size:
5596 3792 1022 694 672
PspXI vC'TCGA_Gb
Cuts at: 7831 8170 7831
Size: 339 11437
PstI C_TGCA'G
Cuts at: 830 2634 4234 4476 4612 6462 7032 7647 8037
Size: 1804 1600 242 136 1850 570 615 390
Cuts at: 8037 8752 8812 8956 9128 830
Size: 715 60 144 172 3478
Fragments arranged by size:
3478 1850 1804 1600 715 615 570 390
242 172 144 136 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11776
PvuII CAG'CTG
Cuts at: 3491 3947 5338 5402 7588 9001 3491
Size: 456 1391 64 2186 1413 6266
Fragments arranged by size:
6266 2186 1413 1391 456 64
SacI G_AGCT'C
Cuts at: 2955 3118 8578 9032 2955
Size: 163 5460 454 5699
Fragments arranged by size:
5699 5460 454 163
SalI G'TCGA_C
Cuts at: 1936 5321 9118 1936
Size: 3385 3797 4594
Fragments arranged by size:
4594 3797 3385
SanDI GG'GwC_CC
Cuts at: 2318 5851 6500 8225 8607 2318
Size: 3533 649 1725 382 5487
Fragments arranged by size:
5487 3533 1725 649 382
SapI GCTCTTCn'nnn_
Cuts at: 4860 7490 8551 4860
Size: 2630 1061 8085
Fragments arranged by size:
8085 2630 1061
SbfI CC_TGCA'GG
Cuts at: 9128 9128
Size: 11776
ScaI AGT'ACT
Cuts at: 5522 10024 11736 5522
Size: 4502 1712 5562
Fragments arranged by size:
5562 4502 1712
SexAI A'CCwGG_T
Cuts at: 6992 8017 9063 6992
Size: 1025 1046 9705
Fragments arranged by size:
9705 1046 1025
SfcI C'TryA_G
Cuts at: 826 1045 2630 3577 3801 4230 4472 4608 5578
Size: 219 1585 947 224 429 242 136 970
Cuts at: 5578 5731 5833 6458 7028 7643 8033 8748 8808
Size: 153 102 625 570 615 390 715 60
Cuts at: 8808 8881 8952 9124 10259 10940 11131 826
Size: 73 71 172 1135 681 191 1471
Fragments arranged by size:
1585 1471 1135 970 947 715 681 625
615 570 429 390 242 224 219 191
172 153 136 102 73 71 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 5160 5160
Size: 11776
SfoI GGC'GCC
Cuts at: 5106 8100 5106
Size: 2994 8782
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11776
SmaI CCC'GGG
Cuts at: 1872 3020 6498 7935 9103 1872
Size: 1148 3478 1437 1168 4545
Fragments arranged by size:
4545 3478 1437 1168 1148
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4376 4996 6845 7831 8170 9031
Size: 197 926 2433 620 1849 986 339 861
Cuts at: 9031 9880 10781 11043 820
Size: 849 901 262 1553
Fragments arranged by size:
2433 1849 1553 986 926 901 861 849
620 339 262 197
SnaBI TAC'GTA
Cuts at: 493 9567 493
Size: 9074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11776
SphI G_CATG'C
Cuts at: 1132 9134 1132
Size: 8002 3774
SspI AAT'ATT
Cuts at: 5 52 3810 9700 5
Size: 47 3758 5890 2081
Fragments arranged by size:
5890 3758 2081 47
StuI AGG'CCT
Cuts at: 1517 4126 6452 8848 9011 1517
Size: 2609 2326 2396 163 4282
Fragments arranged by size:
4282 2609 2396 2326 163
StyI C'CwwG_G
Cuts at: 513 1065 3133 4092 6297 6426 7122 7179 7614
Size: 552 2068 959 2205 129 696 57 435
Cuts at: 7614 8793 9215 513
Size: 1179 422 3074
Fragments arranged by size:
3074 2205 2068 1179 959 696 552 435
422 129 57
SwaI ATTT'AAAT
Cuts at: 8433 8433
Size: 11776
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2426 2568 3066 6536 6680 6872 7370
Size: 1255 846 142 498 3470 144 192 498
Cuts at: 7370 7639 8413 9803 9820 9973 10158 10603 325
Size: 269 774 1390 17 153 185 445 1498
Fragments arranged by size:
3470 1498 1390 1255 846 774 498 498
445 269 192 185 153 144 142 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2195 2903 5028
Size: 274 80 33 51 1443 218 708 2125
Cuts at: 5028 5520 8547 10022 11734 96
Size: 492 3027 1475 1712 138
Fragments arranged by size:
3027 2125 1712 1475 1443 708 492 274
218 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2728 2760 6805 6837 7508 7540 7971 8003 8523
Size: 32 4045 32 671 32 431 32 520
Cuts at: 8523 8555 11736 11768 2728
Size: 32 3181 32 2736
Fragments arranged by size:
4045 3181 2736 671 520 431 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6284 7764 8645 1316
Size: 4968 1480 881 4447
Fragments arranged by size:
4968 4447 1480 881
XbaI T'CTAG_A
Cuts at: 8552 9112 8552
Size: 560 11216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2864 3467 3629 3755 4427 5156 5798 7250 7798
Size: 603 162 126 672 729 642 1452 548
Cuts at: 7798 2864
Size: 6842
Fragments arranged by size:
6842 1452 729 672 642 603 548 162
126
XhoI C'TCGA_G
Cuts at: 1943 6845 7831 8170 1943
Size: 4902 986 339 5549
Fragments arranged by size:
5549 4902 986 339
XmaI C'CCGG_G
Cuts at: 1870 3018 6496 7933 9101 1870
Size: 1148 3478 1437 1168 4545
Fragments arranged by size:
4545 3478 1437 1168 1148
XmnI GAAnn'nnTTC
Cuts at: 3396 9905 3396
Size: 6509 5267
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7156 8590 9584 276
Size: 53 83 186 936 5622 1434 994 2468
Fragments arranged by size:
5622 2468 1434 994 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BstZ17I FseI FspAI
NsiI PacI PsrI RsrII SacII SrfI