Restriction map for
pFN21AA0309
(Circular) (Six-base) MAPSORT of: pFN21AA0309.seq Check: 2878 from: 1 to: 13852
pFN21AA0309 13852 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5107 9616 10030 5107
Size: 4509 414 8929
Fragments arranged by size:
8929 4509 414
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 11032 11662 278
Size: 53 83 186 936 9496 630 2468
Fragments arranged by size:
9496 2468 936 630 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6368 11195 1937
Size: 4431 4827 4594
Fragments arranged by size:
4827 4594 4431
Acc65I G'GTAC_C
Cuts at: 6403 6634 11173 6403
Size: 231 4539 9082
Fragments arranged by size:
9082 4539 231
AclI AA'CG_TT
Cuts at: 1563 3997 11979 12352 1563
Size: 2434 7982 373 3063
Fragments arranged by size:
7982 3063 2434 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2318 3051 3063 3540 4687 5607 5631 6795
Size: 686 733 12 477 1147 920 24 1164
Cuts at: 6795 7242 8160 8790 9393 9437 9561 11004 11915
Size: 447 918 630 603 44 124 1443 911
Cuts at: 11915 13299 13567 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1443 1384 1164 1147 920 918 911
733 686 630 603 477 447 268 124
44 24 12
AfeI AGC'GCT
Cuts at: 4208 8402 4208
Size: 4194 9658
AflII C'TTAA_G
Cuts at: 820 1017 7564 820
Size: 197 6547 7108
Fragments arranged by size:
7108 6547 197
AflIII A'CryG_T
Cuts at: 1203 6058 12751 13728 1203
Size: 4855 6693 977 1327
Fragments arranged by size:
6693 4855 1327 977
AgeI A'CCGG_T
Cuts at: 4666 6400 12683 4666
Size: 1734 6283 5835
Fragments arranged by size:
6283 5835 1734
AhdI GACnn_n'nnGTC
Cuts at: 2674 6024 7366 12581 2674
Size: 3350 1342 5215 3945
Fragments arranged by size:
5215 3945 3350 1342
AleI CACnn'nnGTG
Cuts at: 1166 3965 5570 9791 1166
Size: 2799 1605 4221 5227
Fragments arranged by size:
5227 4221 2799 1605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3303 8382 9809 1184
Size: 2119 5079 1427 5227
Fragments arranged by size:
5227 5079 2119 1427
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7158 7190 8504 8536 7158
Size: 32 1314 32 12474
Fragments arranged by size:
12474 1314 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3311 3431 3707 5024 6314 6502 6655 6697 6823
Size: 120 276 1317 1290 188 153 42 126
Cuts at: 6823 7856 7878 8849 9227 9341 9440 10079 10148
Size: 1033 22 971 378 114 99 639 69
Cuts at: 10148 10178 11595 13167 3311
Size: 30 1417 1572 3996
Fragments arranged by size:
3996 1572 1417 1317 1290 1033 971 639
378 276 188 153 126 120 114 99
69 42 30 22
ApaI G_GGCC'C
Cuts at: 2195 2485 3381 3660 4997 5109 5870 6746 6860
Size: 290 896 279 1337 112 761 876 114
Cuts at: 6860 7201 9885 10209 10891 2195
Size: 341 2684 324 682 5156
Fragments arranged by size:
5156 2684 1337 896 876 761 682 341
324 290 279 114 112
ApaLI G'TGCA_C
Cuts at: 11909 13065 11909
Size: 1156 12696
ApoI r'AATT_y
Cuts at: 1493 8270 8414 11161 11387 11539 13648 1493
Size: 6777 144 2747 226 152 2109 1697
Fragments arranged by size:
6777 2747 2109 1697 226 152 144
AseI AT'TA_AT
Cuts at: 160 1032 12406 160
Size: 872 11374 1606
Fragments arranged by size:
11374 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13852
AvaI C'yCGr_G
Cuts at: 1870 1943 3018 3246 5412 5544 6478 7509 7935
Size: 73 1075 228 2166 132 934 1031 426
Cuts at: 7935 8349 8667 9823 9983 10559 11177 1870
Size: 414 318 1156 160 576 618 4545
Fragments arranged by size:
4545 2166 1156 1075 1031 934 618 576
426 414 318 228 160 132 73
AvrII C'CTAG_G
Cuts at: 8783 8783
Size: 13852
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6957 6990 6957
Size: 33 13819
BamHI G'GATC_C
Cuts at: 11182 11549 11182
Size: 367 13485
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 2314 2448 4002 4833
Size: 325 217 627 345 182 134 1554 831
Cuts at: 4833 4984 5306 5902 5941 6109 6298 6403 6634
Size: 151 322 596 39 168 189 105 231
Cuts at: 6634 6664 6947 7204 7414 8591 9331 10099 11173
Size: 30 283 257 210 1177 740 768 1074
Cuts at: 11173 12628 618
Size: 1455 1842
Fragments arranged by size:
1842 1554 1455 1177 1074 831 768 740
627 596 345 325 322 283 257 231
217 210 189 182 168 151 134 105
39 30
BanII G_rGCy'C
Cuts at: 1391 2195 2325 2485 3054 3381 3660 4039 4997
Size: 804 130 160 569 327 279 379 958
Cuts at: 4997 5031 5109 5468 5551 5870 6485 6746 6836
Size: 34 78 359 83 319 615 261 90
Cuts at: 6836 6860 7201 7503 7516 8457 8668 9423 9885
Size: 24 341 302 13 941 211 755 462
Cuts at: 9885 10209 10891 11171 1391
Size: 324 682 280 4072
Fragments arranged by size:
4072 958 941 804 755 682 615 569
462 379 359 341 327 324 319 302
280 279 261 211 160 130 90 83
78 34 24 13
BbeI G_GCGC'C
Cuts at: 4988 4988
Size: 13852
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3887 4315 6000 8648 928
Size: 971 1988 428 1685 2648 6132
Fragments arranged by size:
6132 2648 1988 1685 971 428
BbvCI CC'TCA_GC
Cuts at: 2743 2958 3192 6391 8331 9345 9430 9549 2743
Size: 215 234 3199 1940 1014 85 119 7046
Fragments arranged by size:
7046 3199 1940 1014 234 215 119 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 10807 10841 12043 12077 1903
Size: 34 8870 34 1202 34 3678
Fragments arranged by size:
8870 3678 1202 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3172 6985 11744 12954 3172
Size: 3813 4759 1210 4070
Fragments arranged by size:
4759 4070 3813 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 13852
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3914 5225 7269
Size: 107 122 71 631 605 2242 1311 2044
Cuts at: 7269 7331 8237 9718 12463 136
Size: 62 906 1481 2745 1525
Fragments arranged by size:
2745 2242 2044 1525 1481 1311 906 631
605 122 107 71 62
BglII A'GATC_T
Cuts at: 9211 13847 9211
Size: 4636 9216
BlpI GC'TnA_GC
Cuts at: 2268 3233 8259 8355 11269 2268
Size: 965 5026 96 2914 4851
Fragments arranged by size:
5026 4851 2914 965 96
Bme1580I G_kGCm'C
Cuts at: 1790 2145 2195 2485 3381 3660 4007 4997 5109
Size: 355 50 290 896 279 347 990 112
Cuts at: 5109 5311 5870 6746 6860 7201 8130 9066 9127
Size: 202 559 876 114 341 929 936 61
Cuts at: 9127 9204 9885 10209 10891 11913 13069 1790
Size: 77 681 324 682 1022 1156 2573
Fragments arranged by size:
2573 1156 1022 990 936 929 896 876
682 681 559 355 347 341 324 290
279 202 114 112 77 61 50
BmgBI CAC'GTC
Cuts at: 1318 7466 1318
Size: 6148 7704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2353 3943 3988
Size: 416 53 83 399 24 936 1590 45
Cuts at: 3988 4997 5048 6689 6836 7546 8837 8849 10136
Size: 1009 51 1641 147 710 1291 12 1287
Cuts at: 10136 10886 11583 12541 442
Size: 750 697 958 1753
Fragments arranged by size:
1753 1641 1590 1291 1287 1009 958 936
750 710 697 416 399 147 83 53
51 45 24 12
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 13852
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4372 8471 10022 10120 4372
Size: 4099 1551 98 8104
Fragments arranged by size:
8104 4099 1551 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2100 2168 2572 2689 2737 3636 3701 4733
Size: 861 68 404 117 48 899 65 1032
Cuts at: 4733 5001 6144 6168 6249 6357 6603 6627 6693
Size: 268 1143 24 81 108 246 24 66
Cuts at: 6693 6711 6720 6759 7148 8123 8204 8441 8453
Size: 18 9 39 389 975 81 237 12
Cuts at: 8453 8784 8988 9126 9173 9437 9485 9499 10053
Size: 331 204 138 47 264 48 14 554
Cuts at: 10053 12512 1239
Size: 2459 2579
Fragments arranged by size:
2579 2459 1143 1032 975 899 861 554
404 389 331 268 264 246 237 204
138 117 108 81 81 68 66 65
48 48 47 39 24 24 18 14
12 9
Bpu10I CC'TnA_GC
Cuts at: 2391 2743 2958 3192 4724 4941 5025 6165 6391
Size: 352 215 234 1532 217 84 1140 226
Cuts at: 6391 7899 8331 9345 9430 9549 2391
Size: 1508 432 1014 85 119 6694
Fragments arranged by size:
6694 1532 1508 1140 1014 432 352 234
226 217 215 119 85 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2076 2618 4040 5570 6850 7610 8664 9121 11977
Size: 542 1422 1530 1280 760 1054 457 2856
Cuts at: 11977 12842 13140 2076
Size: 865 298 2788
Fragments arranged by size:
2856 2788 1530 1422 1280 1054 865 760
542 457 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 9808 10071 10151 10798 10842 12515 882
Size: 617 8309 263 80 647 44 1673 2219
Fragments arranged by size:
8309 2219 1673 647 617 263 80 44
BsaAI yAC'GTr
Cuts at: 493 1204 5668 11643 493
Size: 711 4464 5975 2702
Fragments arranged by size:
5975 4464 2702 711
BsaBI GATnn'nnATC
Cuts at: 4740 13846 4740
Size: 9106 4746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3758 4243 4985 7081
Size: 53 83 186 936 2225 485 742 2096
Cuts at: 7081 9625 11029 11659 12041 12676 275
Size: 2544 1404 630 382 635 1451
Fragments arranged by size:
2544 2225 2096 1451 1404 936 742 635
630 485 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3441 4666 6289 6343 6400 12285 12683 12957 13104
Size: 1225 1623 54 57 5885 398 274 147
Cuts at: 13104 13515 3441
Size: 411 3778
Fragments arranged by size:
5885 3778 1623 1225 411 398 274 147
57 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3974 4004 4233 4246 4263
Size: 30 165 30 2571 30 229 13 17
Cuts at: 4263 4276 4277 4307 6757 6787 7810 7840 8504
Size: 13 1 30 2450 30 1023 30 664
Cuts at: 8504 8534 8950 8980 8986 9016 1178
Size: 30 416 30 6 30 6014
Fragments arranged by size:
6014 2571 2450 1023 664 416 229 165
30 30 30 30 30 30 30 30
30 17 13 13 6 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 2507 3284 3390 3488 3578 4211 5004
Size: 1285 36 777 106 98 90 633 793
Cuts at: 5004 5649 5710 6261 6264 6273 7994 8159 8354
Size: 645 61 551 3 9 1721 165 195
Cuts at: 8354 8450 8465 8528 8552 8555 8558 8904 8955
Size: 96 15 63 24 3 3 346 51
Cuts at: 8955 9027 9175 9395 9609 10985 11012 11015 1186
Size: 72 148 220 214 1376 27 3 4023
Fragments arranged by size:
4023 1721 1376 1285 793 777 645 633
551 346 220 214 195 165 148 106
98 96 90 72 63 61 51 36
27 24 15 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2047 4007 5037 5446 5809 5869 5914 6088 6514
Size: 1960 1030 409 363 60 45 174 426
Cuts at: 6514 6745 6751 6805 6889 6907 7034 7289 7347
Size: 231 6 54 84 18 127 255 58
Cuts at: 7347 8558 9281 9924 9958 10079 10209 10224 10517
Size: 1211 723 643 34 121 130 15 293
Cuts at: 10517 13055 13551 2047
Size: 2538 496 2348
Fragments arranged by size:
2538 2348 1960 1211 1030 723 643 496
426 409 363 293 255 231 174 130
127 121 84 60 58 54 45 34
18 15 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 6836 8247 8941 9261 1163
Size: 573 5100 1411 694 320 5754
Fragments arranged by size:
5754 5100 1411 694 573 320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4667 6864 11302 12063 13091 1540
Size: 275 186 2666 2197 4438 761 1028 2301
Fragments arranged by size:
4438 2666 2301 2197 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5031 5468 7503 7650 8457 9423 10003 11913 11998
Size: 437 2035 147 807 966 580 1910 85
Cuts at: 11998 13069 5031
Size: 1071 5814
Fragments arranged by size:
5814 2035 1910 1071 966 807 580 437
147 85
BsmI GAATG_Cn'
Cuts at: 3852 7677 10692 11363 11456 3852
Size: 3825 3015 671 93 6248
Fragments arranged by size:
6248 3825 3015 671 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4234 9987 4234
Size: 5753 8099
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2195 2325 2485 3054 3381 3660
Size: 399 355 50 130 160 569 327 279
Cuts at: 3660 4007 4039 4997 5031 5109 5311 5468 5551
Size: 347 32 958 34 78 202 157 83
Cuts at: 5551 5870 6485 6746 6836 6860 7201 7503 7516
Size: 319 615 261 90 24 341 302 13
Cuts at: 7516 7650 8130 8457 8668 9066 9127 9204 9423
Size: 134 480 327 211 398 61 77 219
Cuts at: 9423 9885 10003 10209 10891 11171 11913 11998 13069
Size: 462 118 206 682 280 742 85 1071
Cuts at: 13069 1391
Size: 2174
Fragments arranged by size:
2174 1071 958 742 682 615 569 480
462 399 398 355 347 341 327 327
319 302 280 279 261 219 211 206
202 160 157 134 130 118 90 85
83 78 77 61 50 34 32 24
13
BspEI T'CCGG_A
Cuts at: 13515 13515
Size: 13852
BspHI T'CATG_A
Cuts at: 3004 4596 11741 13471 3004
Size: 1592 7145 1730 3385
Fragments arranged by size:
7145 3385 1730 1592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2634 5107 7728 9616 10030 11207 844
Size: 1790 2473 2621 1888 414 1177 3489
Fragments arranged by size:
3489 2621 2473 1888 1790 1177 414
BsrBI CCG'CTC
Cuts at: 1394 1823 6953 11739 1394
Size: 429 5130 4786 3507
Fragments arranged by size:
5130 4786 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2563 2875 3693 7016 9415 9936 12347
Size: 1183 1315 312 818 3323 2399 521 2411
Cuts at: 12347 12521 65
Size: 174 1396
Fragments arranged by size:
3323 2411 2399 1396 1315 1183 818 521
312 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4666 5514 6400 8319 8598 11043 12496
Size: 110 2856 848 886 1919 279 2445 1453
Cuts at: 12496 12683 1700
Size: 187 2869
Fragments arranged by size:
2869 2856 2445 1919 1453 886 848 279
187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 13852
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 13852
BssSI C'ACGA_G
Cuts at: 2453 8640 10296 11912 12924 2453
Size: 6187 1656 1616 1012 3381
Fragments arranged by size:
6187 3381 1656 1616 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6194 6947 7797 8825 794
Size: 5400 753 850 1028 5821
Fragments arranged by size:
5821 5400 1028 850 753
BstBI TT'CG_AA
Cuts at: 9703 12656 9703
Size: 2953 10899
BstEII G'GTnAC_C
Cuts at: 1184 3823 4669 6026 1184
Size: 2639 846 1357 9010
Fragments arranged by size:
9010 2639 1357 846
BstXI CCAn_nnnn'nTGG
Cuts at: 3806 6220 7452 10217 10720 3806
Size: 2414 1232 2765 503 6938
Fragments arranged by size:
6938 2765 2414 1232 503
BstYI r'GATC_y
Cuts at: 1972 7733 9211 9234 10174 11182 11549 11934 11951
Size: 5761 1478 23 940 1008 367 385 17
Cuts at: 11951 13403 13489 13501 13847 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
5761 1977 1478 1452 1008 940 385 367
346 86 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 2310 3057 3402 9108 9594 10956 10990 2310
Size: 747 345 5706 486 1362 34 5172
Fragments arranged by size:
5706 5172 1362 747 486 345 34
BtgI C'CryG_G
Cuts at: 513 1065 2526 3225 3264 3381 4731 4979 5364
Size: 552 1461 699 39 117 1350 248 385
Cuts at: 5364 6081 6969 7705 7890 10713 513
Size: 717 888 736 185 2823 3652
Fragments arranged by size:
3652 2823 1461 1350 888 736 717 699
552 385 248 185 117 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4319 6288 487
Size: 683 56 3093 1969 8051
Fragments arranged by size:
8051 3093 1969 683 56
BtsI GCAGTG_nn'
Cuts at: 2375 3325 3643 5156 7047 7614 9908 10001 10400
Size: 950 318 1513 1891 567 2294 93 399
Cuts at: 10400 10416 11368 12162 12182 2375
Size: 16 952 794 20 4045
Fragments arranged by size:
4045 2294 1891 1513 952 950 794 567
399 318 93 20 16
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 13852
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3899 3934 529
Size: 35 3335 35 10447
Fragments arranged by size:
10447 3335 35 35
DraI TTT'AAA
Cuts at: 4015 11156 4015
Size: 7141 6711
DraIII CAC_nnn'GTG
Cuts at: 7645 7645
Size: 13852
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 12859 809
Size: 507 11543 1802
Fragments arranged by size:
11543 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2529 3738 5512
Size: 54 1425 50 275 18 699 1209 1774
Cuts at: 5512 5988 6126 6861 7708 7927 8028 8160 8317
Size: 476 138 735 847 219 101 132 157
Cuts at: 8317 9168 9671 11299 12188 13817 8
Size: 851 503 1628 889 1629 43
Fragments arranged by size:
1774 1629 1628 1425 1209 889 851 847
735 699 503 476 275 219 157 138
132 101 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6861 11299 1537
Size: 275 5049 4438 4090
Fragments arranged by size:
5049 4438 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 2294 2355 2725 2743 2817 2928 3042
Size: 848 93 61 370 18 74 111 114
Cuts at: 3042 3132 3150 3168 3387 3516 3555 3606 5586
Size: 90 18 18 219 129 39 51 1980
Cuts at: 5586 5659 5701 6058 6291 8003 8136 8145 8208
Size: 73 42 357 233 1712 133 9 63
Cuts at: 8208 8463 9480 10472 10694 11782 1353
Size: 255 1017 992 222 1088 3423
Fragments arranged by size:
3423 1980 1712 1088 1017 992 848 370
357 255 233 222 219 133 129 114
111 93 90 74 73 63 61 51
42 39 18 18 18 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8513 8698 12439 12813 12959 387
Size: 1064 138 6924 185 3741 374 146 1280
Fragments arranged by size:
6924 3741 1280 1064 374 185 146 138
EcoICRI GAG'CTC
Cuts at: 5029 5466 7501 8455 9421 5029
Size: 437 2035 954 966 9460
Fragments arranged by size:
9460 2035 966 954 437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2168 2318 2572 2689 2737 3051
Size: 393 468 68 150 254 117 48 314
Cuts at: 3051 3063 3540 3636 3701 4687 4733 5001 5607
Size: 12 477 96 65 986 46 268 606
Cuts at: 5607 5631 6144 6168 6249 6357 6603 6627 6693
Size: 24 513 24 81 108 246 24 66
Cuts at: 6693 6711 6720 6759 6795 7148 7242 8123 8160
Size: 18 9 39 36 353 94 881 37
Cuts at: 8160 8204 8441 8453 8784 8790 8988 9126 9173
Size: 44 237 12 331 6 198 138 47
Cuts at: 9173 9393 9437 9485 9499 9561 10053 11004 11915
Size: 220 44 48 14 62 492 951 911
Cuts at: 11915 12512 13299 13567 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 986 951 911 881 787 606 597
513 492 477 468 393 353 331 314
268 268 254 246 237 220 198 150
138 117 108 96 94 81 68 66
65 62 48 48 47 46 44 44
39 37 36 24 24 24 18 14
12 12 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3033 3651 4424 5016 7994 3033
Size: 618 773 592 2978 8891
Fragments arranged by size:
8891 2978 773 618 592
EcoO109I rG'GnC_Cy
Cuts at: 2120 2191 2972 3377 3656 3938 4994 5021 5106
Size: 71 781 405 279 282 1056 27 85
Cuts at: 5106 6587 7197 7198 9477 9599 9689 9882 10047
Size: 1481 610 1 2279 122 90 193 165
Cuts at: 10047 10206 10887 10888 10952 11016 2120
Size: 159 681 1 64 64 4956
Fragments arranged by size:
4956 2279 1481 1056 781 681 610 405
282 279 193 165 159 122 90 85
71 64 64 27 1 1
EcoRI G'AATT_C
Cuts at: 11161 11539 11161
Size: 378 13474
EcoRV GAT'ATC
Cuts at: 8221 8221
Size: 13852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3275 3275
Size: 13852
FseI GG_CCGG'CC
Cuts at: 8323 8323
Size: 13852
FspI TGC'GCA
Cuts at: 2435 7547 11087 12358 2435
Size: 5112 3540 1271 3929
Fragments arranged by size:
5112 3929 3540 1271
FspAI rTGC'GCAy
Cuts at: 2435 7547 2435
Size: 5112 8740
HaeII r_GCGC'y
Cuts at: 1678 2401 4159 4210 4988 6884 8404 11097 12999
Size: 723 1758 51 778 1896 1520 2693 1902
Cuts at: 12999 1678
Size: 2531
Fragments arranged by size:
2693 2531 1902 1896 1758 1520 778 723
51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2242 2274 2660 2692 3524
Size: 32 135 32 670 32 386 32 832
Cuts at: 3524 3556 3883 3915 4233 4246 4265 4277 4278
Size: 32 327 32 318 13 19 12 1
Cuts at: 4278 4309 6010 6042 6685 6717 6757 6789 7352
Size: 31 1701 32 643 32 40 32 563
Cuts at: 7352 7384 7635 7667 8330 8362 9464 9496 10328
Size: 32 251 32 663 32 1102 32 832
Cuts at: 10328 10360 12493 12525 12567 12599 1373
Size: 32 2133 32 42 32 2626
Fragments arranged by size:
2626 2133 1701 1102 832 832 670 663
643 563 386 327 318 251 135 42
40 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 31 19 13 12 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4529 4918 5505 5880 11196 11442
Size: 988 281 2591 389 587 375 5316 246
Cuts at: 11442 669
Size: 3079
Fragments arranged by size:
5316 3079 2591 988 587 389 375 281
246
HindIII A'AGCT_T
Cuts at: 748 3842 748
Size: 3094 10758
HpaI GTT'AAC
Cuts at: 5505 11442 5505
Size: 5937 7915
KasI G'GCGC_C
Cuts at: 4984 4984
Size: 13852
KpnI G_GTAC'C
Cuts at: 6407 6638 11177 6407
Size: 231 4539 9082
Fragments arranged by size:
9082 4539 231
MfeI C'AATT_G
Cuts at: 7487 11451 7487
Size: 3964 9888
MluI A'CGCG_T
Cuts at: 13728 13728
Size: 13852
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2384 2946 3045 3183 6437 6620 6710 7201
Size: 1079 562 99 138 3254 183 90 491
Cuts at: 7201 10016 10334 10752 12966 13150 1305
Size: 2815 318 418 2214 184 2007
Fragments arranged by size:
3254 2815 2214 2007 1079 562 491 418
318 184 183 138 99 90
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2531 3740 5990 6128 7710
Size: 54 1425 343 699 1209 2250 138 1582
Cuts at: 7710 7929 8030 8162 9170 10
Size: 219 101 132 1008 4692
Fragments arranged by size:
4692 2250 1582 1425 1209 1008 699 343
219 138 132 101 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3009 3123 3965 4616 5570 7661 9791
Size: 648 1843 114 842 651 954 2091 2130
Cuts at: 9791 10718 11810 12169 12328 518
Size: 927 1092 359 159 2042
Fragments arranged by size:
2130 2091 2042 1843 1092 954 927 842
651 648 359 159 114
MspA1I CmG'CkG
Cuts at: 1606 2464 2747 2962 3191 3707 4509 4624 4753
Size: 858 283 215 229 516 802 115 129
Cuts at: 4753 5347 5480 5918 7150 7366 7914 8386 8745
Size: 594 133 438 1232 216 548 472 359
Cuts at: 8745 9548 9772 11268 11945 13093 13338 1606
Size: 803 224 1496 677 1148 245 2120
Fragments arranged by size:
2120 1496 1232 1148 858 803 802 677
594 548 516 472 438 359 283 245
229 224 216 215 133 129 115
NaeI GCC'GGC
Cuts at: 5516 8321 11045 5516
Size: 2805 2724 8323
Fragments arranged by size:
8323 2805 2724
NarI GG'CG_CC
Cuts at: 4985 4985
Size: 13852
NcoI C'CATG_G
Cuts at: 513 1065 2526 3225 3264 5364 6969 7705 7890
Size: 552 1461 699 39 2100 1605 736 185
Cuts at: 7890 10713 513
Size: 2823 3652
Fragments arranged by size:
3652 2823 2100 1605 1461 736 699 552
185 39
NdeI CA'TA_TG
Cuts at: 387 387
Size: 13852
NgoMIV G'CCGG_C
Cuts at: 5514 8319 11043 5514
Size: 2805 2724 8323
Fragments arranged by size:
8323 2805 2724
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 13852
NotI GC'GGCC_GC
Cuts at: 11299 11299
Size: 13852
NruI TCG'CGA
Cuts at: 4338 4338
Size: 13852
NspI r_CATG'y
Cuts at: 1132 1229 4502 6062 6096 7436 7546 9850 11210
Size: 97 3273 1560 34 1340 110 2304 1360
Cuts at: 11210 12755 1132
Size: 1545 2229
Fragments arranged by size:
3273 2304 2229 1560 1545 1360 1340 110
97 34
PasI CC'CwG_GG
Cuts at: 1348 4990 5684 6583 6853 10148 10313 10594 10948
Size: 3642 694 899 270 3295 165 281 354
Cuts at: 10948 1348
Size: 4252
Fragments arranged by size:
4252 3642 3295 899 694 354 281 270
165
PciI A'CATG_T
Cuts at: 6058 12751 6058
Size: 6693 7159
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3311 7305 9133 10148 10898 1108
Size: 2203 3994 1828 1015 750 4062
Fragments arranged by size:
4062 3994 2203 1828 1015 750
PfoI T'CCnGG_A
Cuts at: 3789 5923 6373 7227 7479 8418 10182 3789
Size: 2134 450 854 252 939 1764 7459
Fragments arranged by size:
7459 2134 1764 939 854 450 252
PmeI GTTT'AAAC
Cuts at: 11156 11156
Size: 13852
PmlI CAC'GTG
Cuts at: 5668 5668
Size: 13852
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8504 8536 11921 11953 13434 13466 8504
Size: 32 3385 32 1481 32 8890
Fragments arranged by size:
8890 3385 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3938 5021 6587 9477 9599 11016 3938
Size: 1083 1566 2890 122 1417 6774
Fragments arranged by size:
6774 2890 1566 1417 1083 122
PshAI GACnn'nnGTC
Cuts at: 1613 4764 7223 7914 8642 1613
Size: 3151 2459 691 728 6823
Fragments arranged by size:
6823 3151 2459 728 691
PsiI TTA'TAA
Cuts at: 11422 11422
Size: 13852
PspOMI G'GGCC_C
Cuts at: 2191 2481 3377 3656 4993 5105 5866 6742 6856
Size: 290 896 279 1337 112 761 876 114
Cuts at: 6856 7197 9881 10205 10887 2191
Size: 341 2684 324 682 5156
Fragments arranged by size:
5156 2684 1337 896 876 761 682 341
324 290 279 114 112
PspXI vC'TCGA_Gb
Cuts at: 5544 5544
Size: 13852
PstI C_TGCA'G
Cuts at: 830 3703 4125 4514 4932 5163 8750 10333 11204
Size: 2873 422 389 418 231 3587 1583 871
Cuts at: 11204 830
Size: 3478
Fragments arranged by size:
3587 3478 2873 1583 871 422 418 389
231
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 13852
PvuII CAG'CTG
Cuts at: 2747 2962 3191 3707 4624 5480 7366 8386 9548
Size: 215 229 516 917 856 1886 1020 1162
Cuts at: 9548 2747
Size: 7051
Fragments arranged by size:
7051 1886 1162 1020 917 856 516 229
215
SacI G_AGCT'C
Cuts at: 5031 5468 7503 8457 9423 5031
Size: 437 2035 954 966 9460
Fragments arranged by size:
9460 2035 966 954 437
SalI G'TCGA_C
Cuts at: 1936 11194 1936
Size: 9258 4594
SanDI GG'GwC_CC
Cuts at: 3938 6587 11016 3938
Size: 2649 4429 6774
Fragments arranged by size:
6774 4429 2649
SapI GCTCTTCn'nnn_
Cuts at: 2201 2725 2743 2928 3042 3387 3606 8463 9480
Size: 524 18 185 114 345 219 4857 1017
Cuts at: 9480 2201
Size: 6573
Fragments arranged by size:
6573 4857 1017 524 345 219 185 114
18
SbfI CC_TGCA'GG
Cuts at: 11204 11204
Size: 13852
ScaI AGT'ACT
Cuts at: 2663 3593 12100 13812 2663
Size: 930 8507 1712 2703
Fragments arranged by size:
8507 2703 1712 930
SexAI A'CCwGG_T
Cuts at: 6022 6022
Size: 13852
SfcI C'TryA_G
Cuts at: 826 1045 3183 3699 4121 4510 4928 5159 5748
Size: 219 2138 516 422 389 418 231 589
Cuts at: 5748 8746 10329 10931 11200 12335 13016 13207 826
Size: 2998 1583 602 269 1135 681 191 1471
Fragments arranged by size:
2998 2138 1583 1471 1135 681 602 589
516 422 418 389 269 231 219 191
SfoI GGC'GCC
Cuts at: 4986 4986
Size: 13852
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13852
SgrAI Cr'CCGG_yG
Cuts at: 1700 8598 1700
Size: 6898 6954
SmaI CCC'GGG
Cuts at: 1872 7511 7937 8351 8669 9825 11179 1872
Size: 5639 426 414 318 1156 1354 4545
Fragments arranged by size:
5639 4545 1354 1156 426 414 318
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2091 2597 3246 4055 5544 5585
Size: 197 926 148 506 649 809 1489 41
Cuts at: 5585 6829 7564 7625 8679 9100 10559 11956 12857
Size: 1244 735 61 1054 421 1459 1397 901
Cuts at: 12857 13119 820
Size: 262 1553
Fragments arranged by size:
1553 1489 1459 1397 1244 1054 926 901
809 735 649 506 421 262 197 148
61 41
SnaBI TAC'GTA
Cuts at: 493 11643 493
Size: 11150 2702
SpeI A'CTAG_T
Cuts at: 152 9812 152
Size: 9660 4192
SphI G_CATG'C
Cuts at: 1132 6096 7436 11210 1132
Size: 4964 1340 3774 3774
Fragments arranged by size:
4964 3774 3774 1340
SrfI GCCC'GGGC
Cuts at: 8669 8669
Size: 13852
SspI AAT'ATT
Cuts at: 5 52 11776 5
Size: 47 11724 2081
Fragments arranged by size:
11724 2081 47
StuI AGG'CCT
Cuts at: 1517 4136 4438 5578 7760 8426 8788 9106 1517
Size: 2619 302 1140 2182 666 362 318 6263
Fragments arranged by size:
6263 2619 2182 1140 666 362 318 302
StyI C'CwwG_G
Cuts at: 513 1065 2526 2532 3113 3225 3264 3723 5364
Size: 552 1461 6 581 112 39 459 1641
Cuts at: 5364 5937 6876 6969 6993 7451 7705 7890 8241
Size: 573 939 93 24 458 254 185 351
Cuts at: 8241 8508 8783 9093 9291 10600 10713 11065 11291
Size: 267 275 310 198 1309 113 352 226
Cuts at: 11291 513
Size: 3074
Fragments arranged by size:
3074 1641 1461 1309 939 581 573 552
459 458 352 351 310 275 267 254
226 198 185 113 112 93 39 24
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5578 6541 7458 7565 7673 8249 8609
Size: 1255 3998 963 917 107 108 576 360
Cuts at: 8609 9295 10389 10483 11879 11896 12049 12234 12679
Size: 686 1094 94 1396 17 153 185 445
Cuts at: 12679 325
Size: 1498
Fragments arranged by size:
3998 1498 1396 1255 1094 963 917 686
576 445 360 185 153 108 107 94
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2661 3591 7336
Size: 274 80 33 51 1443 684 930 3745
Cuts at: 7336 12098 13810 96
Size: 4762 1712 138
Fragments arranged by size:
4762 3745 1712 1443 930 684 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2339 2371 2398 2430 5657 5689 5785 5814 5817
Size: 32 27 32 3227 32 96 29 3
Cuts at: 5817 5846 5895 5927 7810 7842 8950 8982 13812
Size: 29 49 32 1883 32 1108 32 4830
Cuts at: 13812 13844 2339
Size: 32 2347
Fragments arranged by size:
4830 3227 2347 1883 1108 96 49 32
32 32 32 32 32 32 29 29
27 3
Tth111I GACn'n_nGTC
Cuts at: 1316 6607 6655 6697 10142 1316
Size: 5291 48 42 3445 5026
Fragments arranged by size:
5291 5026 3445 48 42
XbaI T'CTAG_A
Cuts at: 11188 11188
Size: 13852
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2143 5045 5530 5566 8416 9289 9640 2143
Size: 2902 485 36 2850 873 351 6355
Fragments arranged by size:
6355 2902 2850 873 485 351 36
XhoI C'TCGA_G
Cuts at: 1943 3246 5544 10559 1943
Size: 1303 2298 5015 5236
Fragments arranged by size:
5236 5015 2298 1303
XmaI C'CCGG_G
Cuts at: 1870 7509 7935 8349 8667 9823 11177 1870
Size: 5639 426 414 318 1156 1354 4545
Fragments arranged by size:
5639 4545 1354 1156 426 414 318
XmnI GAAnn'nnTTC
Cuts at: 2518 11981 2518
Size: 9463 4389
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 11030 11660 276
Size: 53 83 186 936 9496 630 2468
Fragments arranged by size:
9496 2468 936 630 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI BfrBI BsiWI BstZ17I NsiI PacI PsrI RsrII
SacII SfiI SwaI