Restriction map for
pFN21AA0303
(Circular) (Six-base) MAPSORT of: pFN21AA0303.seq Check: 2263 from: 1 to: 11791
pFN21AA0303 11791 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9601 278
Size: 53 83 186 936 8065 2468
Fragments arranged by size:
8065 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2547 3174 9134 1937
Size: 610 627 5960 4594
Fragments arranged by size:
5960 4594 627 610
Acc65I G'GTAC_C
Cuts at: 5307 9112 5307
Size: 3805 7986
AclI AA'CG_TT
Cuts at: 1563 2628 9918 10291 1563
Size: 1065 7290 373 3063
Fragments arranged by size:
7290 3063 1065 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4290 4467 4805 4886 5837 6050 6112 6359
Size: 2658 177 338 81 951 213 62 247
Cuts at: 6359 6635 7119 8367 9854 11238 11506 1632
Size: 276 484 1248 1487 1384 268 1917
Fragments arranged by size:
2658 1917 1487 1384 1248 951 484 338
276 268 247 213 177 81 62
AfeI AGC'GCT
Cuts at: 7175 7476 7175
Size: 301 11490
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11594
AflIII A'CryG_T
Cuts at: 1203 5697 10690 11667 1203
Size: 4494 4993 977 1327
Fragments arranged by size:
4993 4494 1327 977
AgeI A'CCGG_T
Cuts at: 5310 7848 8415 10622 5310
Size: 2538 567 2207 6479
Fragments arranged by size:
6479 2538 2207 567
AhdI GACnn_n'nnGTC
Cuts at: 10520 10520
Size: 11791
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 11791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3225 4250 1184
Size: 2041 1025 8725
Fragments arranged by size:
8725 2041 1025
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6830 6862 6830
Size: 32 11759
AlwNI CAG_nnn'CTG
Cuts at: 3868 4313 4625 5686 6695 7235 8179 8552 9534
Size: 445 312 1061 1009 540 944 373 982
Cuts at: 9534 11106 3868
Size: 1572 4553
Fragments arranged by size:
4553 1572 1061 1009 982 944 540 445
373 312
ApaI G_GGCC'C
Cuts at: 6139 7826 8034 8487 6139
Size: 1687 208 453 9443
Fragments arranged by size:
9443 1687 453 208
ApaLI G'TGCA_C
Cuts at: 4730 9848 11004 4730
Size: 5118 1156 5517
Fragments arranged by size:
5517 5118 1156
ApoI r'AATT_y
Cuts at: 1493 2212 2882 2897 3746 3992 4183 6874 7416
Size: 719 670 15 849 246 191 2691 542
Cuts at: 7416 9100 9326 9478 11587 1493
Size: 1684 226 152 2109 1697
Fragments arranged by size:
2691 2109 1697 1684 849 719 670 542
246 226 191 152 15
AseI AT'TA_AT
Cuts at: 160 1032 3225 10345 160
Size: 872 2193 7120 1606
Fragments arranged by size:
7120 2193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11791
AvaI C'yCGr_G
Cuts at: 1870 1943 2688 3117 5891 7741 7819 8293 8381
Size: 73 745 429 2774 1850 78 474 88
Cuts at: 8381 9116 1870
Size: 735 4545
Fragments arranged by size:
4545 2774 1850 745 735 474 429 88
78 73
BamHI G'GATC_C
Cuts at: 3001 9121 9488 3001
Size: 6120 367 5304
Fragments arranged by size:
6120 5304 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3342 3844 5307 5671 6007
Size: 325 217 627 1555 502 1463 364 336
Cuts at: 6007 6094 6149 7330 7565 9112 10567 618
Size: 87 55 1181 235 1547 1455 1842
Fragments arranged by size:
1842 1555 1547 1463 1455 1181 627 502
364 336 325 235 217 87 55
BanII G_rGCy'C
Cuts at: 1391 2495 5526 6139 6417 6575 6707 6950 7209
Size: 1104 3031 613 278 158 132 243 259
Cuts at: 7209 7737 7748 7826 8034 8062 8487 8872 9110
Size: 528 11 78 208 28 425 385 238
Cuts at: 9110 1391
Size: 4072
Fragments arranged by size:
4072 3031 1104 613 528 425 385 278
259 243 238 208 158 132 78 28
11
BbeI G_GCGC'C
Cuts at: 3346 5675 6011 7334 3346
Size: 2329 336 1323 7803
Fragments arranged by size:
7803 2329 1323 336
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4104 4209 928
Size: 971 2205 105 8510
Fragments arranged by size:
8510 2205 971 105
BbvCI CC'TCA_GC
Cuts at: 2230 4615 8545 2230
Size: 2385 3930 5476
Fragments arranged by size:
5476 3930 2385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7160 7194 9982 10016 1903
Size: 34 5223 34 2788 34 3678
Fragments arranged by size:
5223 3678 2788 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3772 9683 10893 3772
Size: 5911 1210 4670
Fragments arranged by size:
5911 4670 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 11791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3167 6117 10402
Size: 107 122 71 631 605 1495 2950 4285
Cuts at: 10402 136
Size: 1525
Fragments arranged by size:
4285 2950 1525 1495 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4795 5012 11786 4795
Size: 217 6774 4800
Fragments arranged by size:
6774 4800 217
BlpI GC'TnA_GC
Cuts at: 5717 8736 9208 5717
Size: 3019 472 8300
Fragments arranged by size:
8300 3019 472
Bme1580I G_kGCm'C
Cuts at: 1790 3540 4459 4734 5230 5256 6139 6632 7826
Size: 1750 919 275 496 26 883 493 1194
Cuts at: 7826 8034 8487 9852 11008 1790
Size: 208 453 1365 1156 2573
Fragments arranged by size:
2573 1750 1365 1194 1156 919 883 496
493 453 275 208 26
BmgBI CAC'GTC
Cuts at: 1318 5707 6724 8965 1318
Size: 4389 1017 2241 4144
Fragments arranged by size:
4389 4144 2241 1017
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2217 2996 5441
Size: 416 53 83 399 24 800 779 2445
Cuts at: 5441 5753 6380 8562 9522 10480 442
Size: 312 627 2182 960 958 1753
Fragments arranged by size:
2445 2182 1753 960 958 800 779 627
416 399 312 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5033 1056
Size: 3977 7814
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5031 6238 7009 7756 5031
Size: 1207 771 747 9066
Fragments arranged by size:
9066 1207 771 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3768 3920 4279 4425 4812 5645 6140 6854
Size: 2529 152 359 146 387 833 495 714
Cuts at: 6854 6857 7522 8126 8135 8346 8593 8717 8834
Size: 3 665 604 9 211 247 124 117
Cuts at: 8834 10451 1239
Size: 1617 2579
Fragments arranged by size:
2579 2529 1617 833 714 665 604 495
387 359 247 211 152 146 124 117
9 3
Bpu10I CC'TnA_GC
Cuts at: 2230 2638 4615 8545 2230
Size: 408 1977 3930 5476
Fragments arranged by size:
5476 3930 1977 408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2181 5528 6326 6479 7096 9916 10781 11079 2181
Size: 3347 798 153 617 2820 865 298 2893
Fragments arranged by size:
3347 2893 2820 865 798 617 298 153
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2335 4401 5151 6170 6872 7497 8803
Size: 617 836 2066 750 1019 702 625 1306
Cuts at: 8803 10454 882
Size: 1651 2219
Fragments arranged by size:
2219 2066 1651 1306 1019 836 750 702
625 617
BsaAI yAC'GTr
Cuts at: 493 1204 9582 493
Size: 711 8378 2702
Fragments arranged by size:
8378 2702 711
BsaBI GATnn'nnATC
Cuts at: 4649 11785 4649
Size: 7136 4655
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2266 3343 5366 5672
Size: 53 83 186 936 733 1077 2023 306
Cuts at: 5672 6008 7331 8070 9598 9980 10615 275
Size: 336 1323 739 1528 382 635 1451
Fragments arranged by size:
2023 1528 1451 1323 1077 936 739 733
635 382 336 306 186 83 53
BsaWI w'CCGG_w
Cuts at: 2112 2890 5310 5315 6653 7848 8415 10224 10622
Size: 778 2420 5 1338 1195 567 1809 398
Cuts at: 10622 10896 11043 11454 2112
Size: 274 147 411 2449
Fragments arranged by size:
2449 2420 1809 1338 1195 778 567 411
398 274 147 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4809 4839 5356 5386 5555
Size: 30 165 30 3406 30 517 30 169
Cuts at: 5555 5585 5591 5621 6732 6762 6830 6860 8194
Size: 30 6 30 1111 30 68 30 1334
Cuts at: 8194 8224 1178
Size: 30 4745
Fragments arranged by size:
4745 3406 1334 1111 517 169 165 68
30 30 30 30 30 30 30 30
30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 3803 3872 4433 4478 5023 5576 5654
Size: 894 1723 69 561 45 545 553 78
Cuts at: 5654 7256 7387 7849 8052 8596 1186
Size: 1602 131 462 203 544 4381
Fragments arranged by size:
4381 1723 1602 894 561 553 545 544
462 203 131 78 69 45
BseYI C'CCAG_C
Cuts at: 2318 3907 4483 4601 5239 5264 5462 5661 6016
Size: 1589 576 118 638 25 198 199 355
Cuts at: 6016 6707 6752 6857 7435 7610 7721 7736 8711
Size: 691 45 105 578 175 111 15 975
Cuts at: 8711 8920 8925 8935 8980 10994 11490 2318
Size: 209 5 10 45 2014 496 2619
Fragments arranged by size:
2619 2014 1589 975 691 638 578 576
496 355 209 199 198 175 118 111
105 45 45 25 15 10 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5573 5666 6681 7388 8030 1163
Size: 573 3837 93 1015 707 642 4924
Fragments arranged by size:
4924 3837 1015 707 642 573 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6624 7849 8307 9241 10002 11030
Size: 275 186 4623 1225 458 934 761 1028
Cuts at: 11030 1540
Size: 2301
Fragments arranged by size:
4623 2301 1225 1028 934 761 458 275
186
BsiHKAI G_wGCw'C
Cuts at: 2040 4734 5609 6108 9852 9937 11008 2040
Size: 2694 875 499 3744 85 1071 2823
Fragments arranged by size:
3744 2823 2694 1071 875 499 85
BsmI GAATG_Cn'
Cuts at: 6374 9302 9395 6374
Size: 2928 93 8770
Fragments arranged by size:
8770 2928 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3443 6484 8955 3443
Size: 3041 2471 6279
Fragments arranged by size:
6279 3041 2471
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2495 3540 4459 4734 5230 5256
Size: 399 250 455 1045 919 275 496 26
Cuts at: 5256 5526 5609 6108 6139 6417 6575 6632 6707
Size: 270 83 499 31 278 158 57 75
Cuts at: 6707 6950 7209 7737 7748 7826 8034 8062 8487
Size: 243 259 528 11 78 208 28 425
Cuts at: 8487 8872 9110 9852 9937 11008 1391
Size: 385 238 742 85 1071 2174
Fragments arranged by size:
2174 1071 1045 919 742 528 499 496
455 425 399 385 278 275 270 259
250 243 238 208 158 85 83 78
75 57 31 28 26 11
BspEI T'CCGG_A
Cuts at: 2890 5315 6653 11454 2890
Size: 2425 1338 4801 3227
Fragments arranged by size:
4801 3227 2425 1338
BspHI T'CATG_A
Cuts at: 2782 9680 11410 2782
Size: 6898 1730 3163
Fragments arranged by size:
6898 3163 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6840 7046 9146 844
Size: 5996 206 2100 3489
Fragments arranged by size:
5996 3489 2100 206
BsrBI CCG'CTC
Cuts at: 1394 1823 3277 5166 5716 6143 7090 8434 9678
Size: 429 1454 1889 550 427 947 1344 1244
Cuts at: 9678 1394
Size: 3507
Fragments arranged by size:
3507 1889 1454 1344 1244 947 550 429
427
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 2147 3160 3669 6639 7672 10286
Size: 1183 889 10 1013 509 2970 1033 2614
Cuts at: 10286 10460 65
Size: 174 1396
Fragments arranged by size:
2970 2614 1396 1183 1033 1013 889 509
174 10
BsrFI r'CCGG_y
Cuts at: 1700 1810 3709 5310 5333 6421 7848 8302 8387
Size: 110 1899 1601 23 1088 1427 454 85
Cuts at: 8387 8415 10435 10622 1700
Size: 28 2020 187 2869
Fragments arranged by size:
2869 2020 1899 1601 1427 1088 454 187
110 85 28 23
BsrGI T'GTAC_A
Cuts at: 96 3490 96
Size: 3394 8397
BssHII G'CGCG_C
Cuts at: 1925 6061 1925
Size: 4136 7655
BssSI C'ACGA_G
Cuts at: 4587 9851 10863 4587
Size: 5264 1012 5515
Fragments arranged by size:
5515 5264 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3324 4625 8357 794
Size: 2530 1301 3732 4228
Fragments arranged by size:
4228 3732 2530 1301
BstBI TT'CG_AA
Cuts at: 2830 3106 3247 10595 2830
Size: 276 141 7348 4026
Fragments arranged by size:
7348 4026 276 141
BstEII G'GTnAC_C
Cuts at: 1184 3523 4474 5403 5629 1184
Size: 2339 951 929 226 7346
Fragments arranged by size:
7346 2339 951 929 226
BstXI CCAn_nnnn'nTGG
Cuts at: 5830 7358 7697 5830
Size: 1528 339 9924
Fragments arranged by size:
9924 1528 339
BstYI r'GATC_y
Cuts at: 1972 2835 3001 3255 3865 4795 5012 5087 9121
Size: 863 166 254 610 930 217 75 4034
Cuts at: 9121 9488 9873 9890 11342 11428 11440 11786 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
4034 1977 1452 930 863 610 385 367
346 254 217 166 86 75 17 12
Bsu36I CC'TnA_GG
Cuts at: 3884 4470 6660 7130 8466 3884
Size: 586 2190 470 1336 7209
Fragments arranged by size:
7209 2190 1336 586 470
BtgI C'CryG_G
Cuts at: 513 1065 2004 3531 3726 5275 5884 6396 7212
Size: 552 939 1527 195 1549 609 512 816
Cuts at: 7212 7314 8442 8574 8841 513
Size: 102 1128 132 267 3463
Fragments arranged by size:
3463 1549 1527 1128 939 816 609 552
512 267 195 132 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2419 6092 8019 487
Size: 683 56 1193 3673 1927 4259
Fragments arranged by size:
4259 3673 1927 1193 683 56
BtsI GCAGTG_nn'
Cuts at: 5593 6766 7725 8042 9307 10101 10121 5593
Size: 1173 959 317 1265 794 20 7263
Fragments arranged by size:
7263 1265 1173 959 794 317 20
ClaI AT'CG_AT
Cuts at: 1554 3943 6279 1554
Size: 2389 2336 7066
Fragments arranged by size:
7066 2389 2336
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11756
DraI TTT'AAA
Cuts at: 9095 9095
Size: 11791
DraIII CAC_nnn'GTG
Cuts at: 5503 8256 5503
Size: 2753 9038
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10798 809
Size: 507 9482 1802
Fragments arranged by size:
9482 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3429 5729 6270
Size: 54 1425 50 275 18 1599 2300 541
Cuts at: 6270 7071 7638 8014 8304 8435 9238 10127 11756
Size: 801 567 376 290 131 803 889 1629
Cuts at: 11756 8
Size: 43
Fragments arranged by size:
2300 1629 1599 1425 889 803 801 567
541 376 290 275 131 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 8304 9238 1537
Size: 275 6492 934 4090
Fragments arranged by size:
6492 4090 934 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2338 4188 4309 4443 4862 5421 5925 6045
Size: 985 1850 121 134 419 559 504 120
Cuts at: 6045 6563 7517 8674 9721 1353
Size: 518 954 1157 1047 3423
Fragments arranged by size:
3423 1850 1157 1047 985 954 559 518
504 419 134 121 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4984 5320 7614 8342 10378 10752
Size: 1064 138 3395 336 2294 728 2036 374
Cuts at: 10752 10898 387
Size: 146 1280
Fragments arranged by size:
3395 2294 2036 1280 1064 728 374 336
146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3768 3920 4279 4290 4425 4467 4805
Size: 393 2136 152 359 11 135 42 338
Cuts at: 4805 4812 4886 5645 5837 6050 6112 6140 6359
Size: 7 74 759 192 213 62 28 219
Cuts at: 6359 6635 6854 6857 7119 7522 8126 8135 8346
Size: 276 219 3 262 403 604 9 211
Cuts at: 8346 8367 8593 8717 8834 9854 10451 11238 11506
Size: 21 226 124 117 1020 597 787 268
Cuts at: 11506 1239
Size: 1524
Fragments arranged by size:
2136 1524 1020 787 759 604 597 403
393 359 338 276 268 262 226 219
219 213 211 192 152 135 124 117
74 62 42 28 21 11 9 7
3
EcoNI CCTnn'n_nnAGG
Cuts at: 3602 6029 3602
Size: 2427 9364
EcoO109I rG'GnC_Cy
Cuts at: 2716 3410 5532 6136 6729 6926 7151 7281 7377
Size: 694 2122 604 593 197 225 130 96
Cuts at: 7377 7753 7823 8031 8153 8483 8911 2716
Size: 376 70 208 122 330 428 5596
Fragments arranged by size:
5596 2122 694 604 593 428 376 330
225 208 197 130 122 96 70
EcoRI G'AATT_C
Cuts at: 4183 6874 7416 9100 9478 4183
Size: 2691 542 1684 378 6496
Fragments arranged by size:
6496 2691 1684 542 378
EcoRV GAT'ATC
Cuts at: 3283 3283
Size: 11791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3022 6556 7496 7710 3022
Size: 3534 940 214 7103
Fragments arranged by size:
7103 3534 940 214
FseI GG_CCGG'CC
Cuts at: 8391 8391
Size: 11791
FspI TGC'GCA
Cuts at: 7776 10297 7776
Size: 2521 9270
HaeII r_GCGC'y
Cuts at: 1678 3346 5675 6011 7177 7334 7478 10938 1678
Size: 1668 2329 336 1166 157 144 3460 2531
Fragments arranged by size:
3460 2531 2329 1668 1166 336 157 144
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3792 3824 4028 4060 4317
Size: 32 135 32 2220 32 204 32 257
Cuts at: 4317 4349 4785 4817 5168 5200 5356 5388 5555
Size: 32 436 32 351 32 156 32 167
Cuts at: 5555 5587 6429 6461 7382 7414 10432 10464 10506
Size: 32 842 32 921 32 3018 32 42
Cuts at: 10506 10538 1373
Size: 32 2626
Fragments arranged by size:
3018 2626 2220 921 842 436 351 257
204 167 156 135 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 9135 9381 669
Size: 988 281 7197 246 3079
Fragments arranged by size:
7197 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2119 7028 7101 748
Size: 1371 4909 73 5438
Fragments arranged by size:
5438 4909 1371 73
HpaI GTT'AAC
Cuts at: 9381 9381
Size: 11791
KasI G'GCGC_C
Cuts at: 3342 5671 6007 7330 3342
Size: 2329 336 1323 7803
Fragments arranged by size:
7803 2329 1323 336
KpnI G_GTAC'C
Cuts at: 5311 9116 5311
Size: 3805 7986
MfeI C'AATT_G
Cuts at: 9390 9390
Size: 11791
MluI A'CGCG_T
Cuts at: 11667 11667
Size: 11791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3736 4323 5174 5399 6063 6415 10905 11089
Size: 2431 587 851 225 664 352 4490 184
Cuts at: 11089 1305
Size: 2007
Fragments arranged by size:
4490 2431 2007 851 664 587 352 225
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3431 6272 7073 8016 10
Size: 54 1425 343 1599 2841 801 943 3785
Fragments arranged by size:
3785 2841 1599 1425 943 801 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2465 3359 3480 4697 4812 7356
Size: 648 843 456 894 121 1217 115 2544
Cuts at: 7356 9749 10108 10267 518
Size: 2393 359 159 2042
Fragments arranged by size:
2544 2393 2042 1217 894 843 648 456
359 159 121 115
MspA1I CmG'CkG
Cuts at: 1606 2271 5305 5356 5401 5539 5650 5665 5677
Size: 665 3034 51 45 138 111 15 12
Cuts at: 5677 6016 6356 7412 8168 8186 8444 9207 9884
Size: 339 340 1056 756 18 258 763 677
Cuts at: 9884 11032 11277 1606
Size: 1148 245 2120
Fragments arranged by size:
3034 2120 1148 1056 763 756 677 665
340 339 258 245 138 111 51 45
18 15 12
NaeI GCC'GGC
Cuts at: 5335 6423 8304 8389 5335
Size: 1088 1881 85 8737
Fragments arranged by size:
8737 1881 1088 85
NarI GG'CG_CC
Cuts at: 3343 5672 6008 7331 3343
Size: 2329 336 1323 7803
Fragments arranged by size:
7803 2329 1323 336
NcoI C'CATG_G
Cuts at: 513 1065 2004 3531 3726 5884 7212 7314 8841
Size: 552 939 1527 195 2158 1328 102 1527
Cuts at: 8841 513
Size: 3463
Fragments arranged by size:
3463 2158 1527 1527 1328 939 552 195
102
NdeI CA'TA_TG
Cuts at: 387 3925 4062 387
Size: 3538 137 8116
Fragments arranged by size:
8116 3538 137
NgoMIV G'CCGG_C
Cuts at: 5333 6421 8302 8387 5333
Size: 1088 1881 85 8737
Fragments arranged by size:
8737 1881 1088 85
NheI G'CTAG_C
Cuts at: 1052 5029 1052
Size: 3977 7814
NotI GC'GGCC_GC
Cuts at: 9238 9238
Size: 11791
NspI r_CATG'y
Cuts at: 1132 1229 3322 4773 7200 8695 9149 10694 1132
Size: 97 2093 1451 2427 1495 454 1545 2229
Fragments arranged by size:
2427 2229 2093 1545 1495 1451 454 97
PasI CC'CwG_GG
Cuts at: 1348 8150 1348
Size: 6802 4989
PciI A'CATG_T
Cuts at: 10690 10690
Size: 11791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 3773 4829 7315 1108
Size: 920 1745 1056 2486 5584
Fragments arranged by size:
5584 2486 1745 1056 920
PfoI T'CCnGG_A
Cuts at: 3636 5765 6156 8583 3636
Size: 2129 391 2427 6844
Fragments arranged by size:
6844 2427 2129 391
PmeI GTTT'AAAC
Cuts at: 9095 9095
Size: 11791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4246 4278 6830 6862 9860 9892 11373 11405 4246
Size: 32 2552 32 2998 32 1481 32 4632
Fragments arranged by size:
4632 2998 2552 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3410 5532 6926 7281 7753 8911 3410
Size: 2122 1394 355 472 1158 6290
Fragments arranged by size:
6290 2122 1394 1158 472 355
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11791
PsiI TTA'TAA
Cuts at: 9361 9361
Size: 11791
PspOMI G'GGCC_C
Cuts at: 6135 7822 8030 8483 6135
Size: 1687 208 453 9443
Fragments arranged by size:
9443 1687 453 208
PspXI vC'TCGA_Gb
Cuts at: 7741 8381 7741
Size: 640 11151
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2531 2563 2531
Size: 32 11759
PstI C_TGCA'G
Cuts at: 830 5682 7500 7732 8653 9143 830
Size: 4852 1818 232 921 490 3478
Fragments arranged by size:
4852 3478 1818 921 490 232
PvuI CG_AT'CG
Cuts at: 2001 6624 2001
Size: 4623 7168
PvuII CAG'CTG
Cuts at: 6356 7412 6356
Size: 1056 10735
RsrII CG'GwC_CG
Cuts at: 5313 5313
Size: 11791
SacII CC_GC'GG
Cuts at: 8445 8445
Size: 11791
SalI G'TCGA_C
Cuts at: 1936 9133 1936
Size: 7197 4594
SapI GCTCTTCn'nnn_
Cuts at: 6045 6563 6045
Size: 518 11273
SbfI CC_TGCA'GG
Cuts at: 9143 9143
Size: 11791
ScaI AGT'ACT
Cuts at: 5977 10039 11751 5977
Size: 4062 1712 6017
Fragments arranged by size:
6017 4062 1712
SfcI C'TryA_G
Cuts at: 826 1045 2571 2771 5678 7496 7728 8649 9139
Size: 219 1526 200 2907 1818 232 921 490
Cuts at: 9139 10274 10955 11146 826
Size: 1135 681 191 1471
Fragments arranged by size:
2907 1818 1526 1471 1135 921 681 490
232 219 200 191
SfoI GGC'GCC
Cuts at: 3344 5673 6009 7332 3344
Size: 2329 336 1323 7803
Fragments arranged by size:
7803 2329 1323 336
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11791
SmaI CCC'GGG
Cuts at: 1872 5893 7821 9118 1872
Size: 4021 1928 1297 4545
Fragments arranged by size:
4545 4021 1928 1297
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2196 2688 5543 6305 6494 7111
Size: 197 926 253 492 2855 762 189 617
Cuts at: 7111 7741 8381 9895 10796 11058 820
Size: 630 640 1514 901 262 1553
Fragments arranged by size:
2855 1553 1514 926 901 762 640 630
617 492 262 253 197 189
SnaBI TAC'GTA
Cuts at: 493 9582 493
Size: 9089 2702
SpeI A'CTAG_T
Cuts at: 152 2824 152
Size: 2672 9119
SphI G_CATG'C
Cuts at: 1132 4773 8695 9149 1132
Size: 3641 3922 454 3774
Fragments arranged by size:
3922 3774 3641 454
SspI AAT'ATT
Cuts at: 5 52 3470 4271 9715 5
Size: 47 3418 801 5444 2081
Fragments arranged by size:
5444 3418 2081 801 47
StuI AGG'CCT
Cuts at: 1517 3266 6500 6665 7128 8645 9086 1517
Size: 1749 3234 165 463 1517 441 4222
Fragments arranged by size:
4222 3234 1749 1517 463 441 165
StyI C'CwwG_G
Cuts at: 513 1065 2004 3006 3462 3531 3726 5823 5884
Size: 552 939 1002 456 69 195 2097 61
Cuts at: 5884 6231 6842 7212 7314 7569 8135 8676 8841
Size: 347 611 370 102 255 566 541 165
Cuts at: 8841 9087 9230 513
Size: 246 143 3074
Fragments arranged by size:
3074 2097 1002 939 611 566 552 541
456 370 347 255 246 195 165 143
102 69 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2332 4032 5302 6470 7302 8163 9818
Size: 1255 752 1700 1270 1168 832 861 1655
Cuts at: 9818 9835 9988 10173 10618 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1700 1655 1498 1270 1255 1168 861 832
752 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3438 3490 5975
Size: 274 80 33 51 1443 1461 52 2485
Cuts at: 5975 10037 11749 96
Size: 4062 1712 138
Fragments arranged by size:
4062 2485 1712 1461 1443 274 138 80
52 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2721 2753 3696 3728 4809 4841 5591 5623 5864
Size: 32 943 32 1081 32 750 32 241
Cuts at: 5864 5896 6732 6764 11751 11783 2721
Size: 32 836 32 4987 32 2729
Fragments arranged by size:
4987 2729 1081 943 836 750 241 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11791
XbaI T'CTAG_A
Cuts at: 8065 9127 8065
Size: 1062 10729
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5882 7952 8024 8809 5882
Size: 2070 72 785 8864
Fragments arranged by size:
8864 2070 785 72
XhoI C'TCGA_G
Cuts at: 1943 2688 7741 8381 1943
Size: 745 5053 640 5353
Fragments arranged by size:
5353 5053 745 640
XmaI C'CCGG_G
Cuts at: 1870 5891 7819 9116 1870
Size: 4021 1928 1297 4545
Fragments arranged by size:
4545 4021 1928 1297
XmnI GAAnn'nnTTC
Cuts at: 3684 3996 7476 8459 9920 3684
Size: 312 3480 983 1461 5555
Fragments arranged by size:
5555 3480 1461 983 312
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9599 276
Size: 53 83 186 936 8065 2468
Fragments arranged by size:
8065 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI
FspAI NruI NsiI PacI PmlI SacI SanDI SexAI
SfiI SrfI SwaI