Restriction map for
pFN21AA0294
(Circular) (Six-base) MAPSORT of: pFN21AA0294.seq Check: 7611 from: 1 to: 8737
pFN21AA0294 8737 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5364 5364
Size: 8737
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6547 278
Size: 53 83 186 936 5011 2468
Fragments arranged by size:
5011 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3219 5146 6080 1937
Size: 1282 1927 934 4594
Fragments arranged by size:
4594 1927 1282 934
Acc65I G'GTAC_C
Cuts at: 6058 6058
Size: 8737
AclI AA'CG_TT
Cuts at: 1563 6864 7237 1563
Size: 5301 373 3063
Fragments arranged by size:
5301 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2981 3341 3683 3686 4037 4955 5879 6800
Size: 1349 360 342 3 351 918 924 921
Cuts at: 6800 8184 8452 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1349 924 921 918 360 351
342 268 3
AfeI AGC'GCT
Cuts at: 2338 2338
Size: 8737
AflII C'TTAA_G
Cuts at: 820 1017 3248 820
Size: 197 2231 6309
Fragments arranged by size:
6309 2231 197
AflIII A'CryG_T
Cuts at: 1203 3524 4039 5163 7636 8613 1203
Size: 2321 515 1124 2473 977 1327
Fragments arranged by size:
2473 2321 1327 1124 977 515
AgeI A'CCGG_T
Cuts at: 7568 7568
Size: 8737
AhdI GACnn_n'nnGTC
Cuts at: 7466 7466
Size: 8737
AleI CACnn'nnGTG
Cuts at: 1166 2231 2327 3503 1166
Size: 1065 96 1176 6400
Fragments arranged by size:
6400 1176 1065 96
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8737
AlwNI CAG_nnn'CTG
Cuts at: 2675 5032 5413 6480 8052 2675
Size: 2357 381 1067 1572 3360
Fragments arranged by size:
3360 2357 1572 1067 381
ApaLI G'TGCA_C
Cuts at: 5957 6794 7950 5957
Size: 837 1156 6744
Fragments arranged by size:
6744 1156 837
ApoI r'AATT_y
Cuts at: 1493 2762 3109 3551 4359 4753 5567 6046 6272
Size: 1269 347 442 808 394 814 479 226
Cuts at: 6272 6424 8533 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1269 814 808 479 442 394
347 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7291 160
Size: 872 6259 1606
Fragments arranged by size:
6259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8737
AvaI C'yCGr_G
Cuts at: 1870 1943 3164 4376 4862 5639 6062 1870
Size: 73 1221 1212 486 777 423 4545
Fragments arranged by size:
4545 1221 1212 777 486 423 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4160 4193 4160
Size: 33 8704
BamHI G'GATC_C
Cuts at: 5087 5863 6067 6434 5087
Size: 776 204 367 7390
Fragments arranged by size:
7390 776 367 204
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2383 2407 2996 4092 4849
Size: 325 217 627 596 24 589 1096 757
Cuts at: 4849 5403 5459 5995 6058 7513 618
Size: 554 56 536 63 1455 1842
Fragments arranged by size:
1842 1455 1096 757 627 596 589 554
536 325 217 63 56 24
BanII G_rGCy'C
Cuts at: 1391 2022 3723 5080 6056 1391
Size: 631 1701 1357 976 4072
Fragments arranged by size:
4072 1701 1357 976 631
BbeI G_GCGC'C
Cuts at: 5999 5999
Size: 8737
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3329 3424 3919 4533 5166 5185 5661
Size: 971 1430 95 495 614 633 19 476
Cuts at: 5661 928
Size: 4004
Fragments arranged by size:
4004 1430 971 633 614 495 476 95
19
BbvCI CC'TCA_GC
Cuts at: 2340 2340
Size: 8737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6928 6962 1903
Size: 34 4991 34 3678
Fragments arranged by size:
4991 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6629 7839 6629
Size: 1210 7527
BclI T'GATC_A
Cuts at: 1548 3766 5115 1548
Size: 2218 1349 5170
Fragments arranged by size:
5170 2218 1349
BfrBI ATG'CAT
Cuts at: 3470 3470
Size: 8737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2591 5918 7348
Size: 107 122 71 631 605 919 3327 1430
Cuts at: 7348 136
Size: 1525
Fragments arranged by size:
3327 1525 1430 919 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3086 8732 3086
Size: 5646 3091
BlpI GC'TnA_GC
Cuts at: 6154 6154
Size: 8737
Bme1580I G_kGCm'C
Cuts at: 1790 2334 2370 2412 4852 5406 5961 6798 7954
Size: 544 36 42 2440 554 555 837 1156
Cuts at: 7954 1790
Size: 2573
Fragments arranged by size:
2573 2440 1156 837 555 554 544 42
36
BmgBI CAC'GTC
Cuts at: 1318 2469 5022 5498 1318
Size: 1151 2553 476 4557
Fragments arranged by size:
4557 2553 1151 476
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2225 3215 4043
Size: 416 53 83 399 24 808 990 828
Cuts at: 4043 6468 7426 442
Size: 2425 958 1753
Fragments arranged by size:
2425 1753 990 958 828 808 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3424 5817 3424
Size: 2393 6344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2190 2235 2428 3032 3635 5255 5794 7397
Size: 951 45 193 604 603 1620 539 1603
Cuts at: 7397 1239
Size: 2579
Fragments arranged by size:
2579 1620 1603 951 604 603 539 193
45
Bpu10I CC'TnA_GC
Cuts at: 2340 2942 5792 2340
Size: 602 2850 5285
Fragments arranged by size:
5285 2850 602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3116 3500 5333 6862 7727 8025 3116
Size: 384 1833 1529 865 298 3828
Fragments arranged by size:
3828 1833 1529 865 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2363 4859 5542 7400 882
Size: 617 864 2496 683 1858 2219
Fragments arranged by size:
2496 2219 1858 864 683 617
BsaAI yAC'GTr
Cuts at: 493 1204 3238 6528 493
Size: 711 2034 3290 2702
Fragments arranged by size:
3290 2702 2034 711
BsaBI GATnn'nnATC
Cuts at: 3765 4536 4736 8731 3765
Size: 771 200 3995 3771
Fragments arranged by size:
3995 3771 771 200
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4047 5127 5286 5996
Size: 53 83 186 936 2514 1080 159 710
Cuts at: 5996 6544 6926 7561 275
Size: 548 382 635 1451
Fragments arranged by size:
2514 1451 1080 936 710 635 548 382
186 159 83 53
BsaWI w'CCGG_w
Cuts at: 5180 5522 7170 7568 7842 7989 8400 5180
Size: 342 1648 398 274 147 411 5517
Fragments arranged by size:
5517 1648 411 398 342 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2743 2773 3888 3918 5175
Size: 30 165 30 1340 30 1115 30 1257
Cuts at: 5175 5205 5463 5493 5759 5789 1178
Size: 30 258 30 266 30 4126
Fragments arranged by size:
4126 1340 1257 1115 266 258 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2357 2360 2549 3909 4511 4556 4841 4931
Size: 1171 3 189 1360 602 45 285 90
Cuts at: 4931 5697 5767 1186
Size: 766 70 4156
Fragments arranged by size:
4156 1360 1171 766 602 285 189 90
70 45 3
BseYI C'CCAG_C
Cuts at: 2333 2589 5743 7940 8436 2333
Size: 256 3154 2197 496 2634
Fragments arranged by size:
3154 2634 2197 496 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2172 5576 1163
Size: 573 436 3404 4324
Fragments arranged by size:
4324 3404 573 436
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2566 3586 4403 4875 6003 6187
Size: 275 186 565 1020 817 472 1128 184
Cuts at: 6187 6948 7976 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1128 1028 1020 817 761 565 472
275 186 184
BsiHKAI G_wGCw'C
Cuts at: 2259 2880 3723 4086 5826 5961 6798 6883 7954
Size: 621 843 363 1740 135 837 85 1071
Cuts at: 7954 2259
Size: 3042
Fragments arranged by size:
3042 1740 1071 843 837 621 363 135
85
BsmI GAATG_Cn'
Cuts at: 6248 6341 6248
Size: 93 8644
BsmBI CGTCTCn'nnnn_
Cuts at: 4747 5028 4747
Size: 281 8456
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2259 2334 2370 2412 2880 3723
Size: 399 232 237 75 36 42 468 843
Cuts at: 3723 4086 4852 5080 5406 5826 5961 6056 6798
Size: 363 766 228 326 420 135 95 742
Cuts at: 6798 6883 7954 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 843 766 742 468 420 399
363 326 237 232 228 135 95 85
75 42 36
BspEI T'CCGG_A
Cuts at: 5180 8400 5180
Size: 3220 5517
BspHI T'CATG_A
Cuts at: 2854 3979 5004 5151 6626 8356 2854
Size: 1125 1025 147 1475 1730 3235
Fragments arranged by size:
3235 1730 1475 1125 1025 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2427 3175 5364 6092 844
Size: 1583 748 2189 728 3489
Fragments arranged by size:
3489 2189 1583 748 728
BsrBI CCG'CTC
Cuts at: 1394 1823 2033 3045 6624 1394
Size: 429 210 1012 3579 3507
Fragments arranged by size:
3579 3507 1012 429 210
BsrDI GCAATG_nn'
Cuts at: 65 1248 7232 7406 65
Size: 1183 5984 174 1396
Fragments arranged by size:
5984 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5488 7381 7568 1700
Size: 110 3678 1893 187 2869
Fragments arranged by size:
3678 2869 1893 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8737
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8737
BssSI C'ACGA_G
Cuts at: 2975 5477 5594 6797 7809 2975
Size: 2502 117 1203 1012 3903
Fragments arranged by size:
3903 2502 1203 1012 117
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4803 794
Size: 4009 4728
BstBI TT'CG_AA
Cuts at: 7541 7541
Size: 8737
BstEII G'GTnAC_C
Cuts at: 1184 5708 1184
Size: 4524 4213
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 2473 3463 5731 2233
Size: 240 990 2268 5239
Fragments arranged by size:
5239 2268 990 240
BstYI r'GATC_y
Cuts at: 1972 3086 3348 5087 5292 5863 6018 6067 6434
Size: 1114 262 1739 205 571 155 49 367
Cuts at: 6434 6819 6836 8288 8374 8386 8732 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1739 1452 1114 571 385 367 346
262 205 155 86 49 17 12
Bsu36I CC'TnA_GG
Cuts at: 5311 5646 5311
Size: 335 8402
BtgI C'CryG_G
Cuts at: 513 1065 2004 3411 3456 3504 4125 4680 5440
Size: 552 939 1407 45 48 621 555 760
Cuts at: 5440 5583 513
Size: 143 3667
Fragments arranged by size:
3667 1407 939 760 621 555 552 143
48 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3055 3441 5356 487
Size: 683 56 1829 386 1915 3868
Fragments arranged by size:
3868 1915 1829 683 386 56
BtsI GCAGTG_nn'
Cuts at: 3362 3841 6253 7047 7067 3362
Size: 479 2412 794 20 5032
Fragments arranged by size:
5032 2412 794 479 20
ClaI AT'CG_AT
Cuts at: 1554 5750 1554
Size: 4196 4541
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2898 2933 529
Size: 35 2334 35 6333
Fragments arranged by size:
6333 2334 35 35
DraI TTT'AAA
Cuts at: 2871 3558 6041 2871
Size: 687 2483 5567
Fragments arranged by size:
5567 2483 687
DraIII CAC_nnn'GTG
Cuts at: 5169 5169
Size: 8737
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7744 809
Size: 507 6428 1802
Fragments arranged by size:
6428 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2604 3384 4122
Size: 54 1425 50 275 18 774 780 738
Cuts at: 4122 4219 4476 4593 4872 5274 5580 6184 7073
Size: 97 257 117 279 402 306 604 889
Cuts at: 7073 8702 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 780 774 738 604 402
306 279 275 257 117 97 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4872 6184 1537
Size: 275 3060 1312 4090
Fragments arranged by size:
4090 3060 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 2076 2772 3029 3705 4488 5521 5982
Size: 717 6 696 257 676 783 1033 461
Cuts at: 5982 6667 1353
Size: 685 3423
Fragments arranged by size:
3423 1033 783 717 696 685 676 461
257 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2137 5315 7324 7698 7844 387
Size: 1064 138 548 3178 2009 374 146 1280
Fragments arranged by size:
3178 2009 1280 1064 548 374 146 138
EcoICRI GAG'CTC
Cuts at: 3721 3721
Size: 8737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2235 2428 2981 3032 3341 3635
Size: 393 558 45 193 553 51 309 294
Cuts at: 3635 3683 3686 4037 4955 5255 5794 5879 6800
Size: 48 3 351 918 300 539 85 921
Cuts at: 6800 7397 8184 8452 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 921 918 787 597 558 553 539
393 351 309 300 294 268 193 85
51 48 45 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2157 3052 3650 4493 2157
Size: 895 598 843 6401
Fragments arranged by size:
6401 895 843 598
EcoO109I rG'GnC_Cy
Cuts at: 2140 2817 5787 2140
Size: 677 2970 5090
Fragments arranged by size:
5090 2970 677
EcoRI G'AATT_C
Cuts at: 4359 6046 6424 4359
Size: 1687 378 6672
Fragments arranged by size:
6672 1687 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4985 4985
Size: 8737
FspI TGC'GCA
Cuts at: 2828 7243 2828
Size: 4415 4322
HaeII r_GCGC'y
Cuts at: 1678 2340 5924 5999 7884 1678
Size: 662 3584 75 1885 2531
Fragments arranged by size:
3584 2531 1885 662 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2103 2135 2611 2643 3049
Size: 32 135 32 531 32 476 32 406
Cuts at: 3049 3071 3081 3103 3158 3190 4280 4312 5175
Size: 22 10 22 55 32 1090 32 863
Cuts at: 5175 5207 5520 5552 5759 5791 5813 5845 5855
Size: 32 313 32 207 32 22 32 10
Cuts at: 5855 5887 7378 7410 7452 7484 1373
Size: 32 1491 32 42 32 2626
Fragments arranged by size:
2626 1491 1090 863 531 476 406 313
207 135 55 42 32 32 32 32
32 32 32 32 32 32 32 32
32 22 22 22 10 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2272 3127 3220 4462 5050 6081
Size: 988 281 334 855 93 1242 588 1031
Cuts at: 6081 6327 669
Size: 246 3079
Fragments arranged by size:
3079 1242 1031 988 855 588 334 281
246 93
HindIII A'AGCT_T
Cuts at: 748 3824 748
Size: 3076 5661
HpaI GTT'AAC
Cuts at: 6327 6327
Size: 8737
KasI G'GCGC_C
Cuts at: 5995 5995
Size: 8737
KpnI G_GTAC'C
Cuts at: 6062 6062
Size: 8737
MfeI C'AATT_G
Cuts at: 6336 6336
Size: 8737
MluI A'CGCG_T
Cuts at: 8613 8613
Size: 8737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2439 2567 4552 7851 8035 1305
Size: 1134 128 1985 3299 184 2007
Fragments arranged by size:
3299 2007 1985 1134 184 128
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3386 4221 4478 4595 5582
Size: 54 1425 343 1554 835 257 117 987
Cuts at: 5582 10
Size: 3165
Fragments arranged by size:
3165 1554 1425 987 835 343 257 117
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2327 3503 4583 5003 5204 5276
Size: 648 1065 96 1176 1080 420 201 72
Cuts at: 5276 6695 7054 7213 518
Size: 1419 359 159 2042
Fragments arranged by size:
2042 1419 1176 1080 1065 648 420 359
201 159 96 72
MspA1I CmG'CkG
Cuts at: 1606 2589 3280 4234 4418 5233 5410 6153 6830
Size: 983 691 954 184 815 177 743 677
Cuts at: 6830 7978 8223 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 983 954 815 743 691 677
245 184 177
NarI GG'CG_CC
Cuts at: 5996 5996
Size: 8737
NcoI C'CATG_G
Cuts at: 513 1065 2004 3456 513
Size: 552 939 1452 5794
Fragments arranged by size:
5794 1452 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8737
NotI GC'GGCC_GC
Cuts at: 6184 6184
Size: 8737
NsiI A_TGCA'T
Cuts at: 3472 3472
Size: 8737
NspI r_CATG'y
Cuts at: 1132 1229 3528 3661 4043 4582 4810 5395 6095
Size: 97 2299 133 382 539 228 585 700
Cuts at: 6095 7640 1132
Size: 1545 2229
Fragments arranged by size:
2299 2229 1545 700 585 539 382 228
133 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8737
PciI A'CATG_T
Cuts at: 3524 4039 7636 3524
Size: 515 3597 4625
Fragments arranged by size:
4625 3597 515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4723 1108
Size: 3615 5122
PfoI T'CCnGG_A
Cuts at: 2304 3651 2304
Size: 1347 7390
PmeI GTTT'AAAC
Cuts at: 6041 6041
Size: 8737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6806 6838 8319 8351 6806
Size: 32 1481 32 7192
Fragments arranged by size:
7192 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2817 5787 2817
Size: 2970 5767
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8737
PsiI TTA'TAA
Cuts at: 4632 4938 6307 4632
Size: 306 1369 7062
Fragments arranged by size:
7062 1369 306
PstI C_TGCA'G
Cuts at: 830 2041 3357 4358 5361 6089 830
Size: 1211 1316 1001 1003 728 3478
Fragments arranged by size:
3478 1316 1211 1003 1001 728
PvuI CG_AT'CG
Cuts at: 2001 3586 2001
Size: 1585 7152
PvuII CAG'CTG
Cuts at: 4234 4418 5233 4234
Size: 184 815 7738
Fragments arranged by size:
7738 815 184
SacI G_AGCT'C
Cuts at: 3723 3723
Size: 8737
SalI G'TCGA_C
Cuts at: 1936 3218 6079 1936
Size: 1282 2861 4594
Fragments arranged by size:
4594 2861 1282
SanDI GG'GwC_CC
Cuts at: 5787 5787
Size: 8737
SapI GCTCTTCn'nnn_
Cuts at: 2772 5982 2772
Size: 3210 5527
SbfI CC_TGCA'GG
Cuts at: 5361 6089 5361
Size: 728 8009
ScaI AGT'ACT
Cuts at: 6985 8697 6985
Size: 1712 7025
SfcI C'TryA_G
Cuts at: 826 1045 2037 3353 3598 4354 5220 5357 6085
Size: 219 992 1316 245 756 866 137 728
Cuts at: 6085 7220 7901 8092 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1316 1135 992 866 756 728 681
245 219 191 137
SfoI GGC'GCC
Cuts at: 5997 5997
Size: 8737
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8737
SmaI CCC'GGG
Cuts at: 1872 4378 6064 1872
Size: 2506 1686 4545
Fragments arranged by size:
4545 2506 1686
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3095 3248 3515 5348 6841 7742
Size: 197 926 1152 153 267 1833 1493 901
Cuts at: 7742 8004 820
Size: 262 1553
Fragments arranged by size:
1833 1553 1493 1152 926 901 267 262
197 153
SnaBI TAC'GTA
Cuts at: 493 3238 6528 493
Size: 2745 3290 2702
Fragments arranged by size:
3290 2745 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8737
SphI G_CATG'C
Cuts at: 1132 4810 6095 1132
Size: 3678 1285 3774
Fragments arranged by size:
3774 3678 1285
SspI AAT'ATT
Cuts at: 5 52 6661 5
Size: 47 6609 2081
Fragments arranged by size:
6609 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8737
StyI C'CwwG_G
Cuts at: 513 1065 2004 2657 2736 3000 3456 5907 6176
Size: 552 939 653 79 264 456 2451 269
Cuts at: 6176 513
Size: 3074
Fragments arranged by size:
3074 2451 939 653 552 456 269 264
79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2206 2552 4417 4730 6764 6781 6934
Size: 1255 626 346 1865 313 2034 17 153
Cuts at: 6934 7119 7564 325
Size: 185 445 1498
Fragments arranged by size:
2034 1865 1498 1255 626 445 346 313
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6983 8695 96
Size: 274 80 33 51 1443 5006 1712 138
Fragments arranged by size:
5006 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2743 2775 3624 3656 4677 4709 5463 5495 8697
Size: 32 849 32 1021 32 754 32 3202
Cuts at: 8697 8729 2743
Size: 32 2751
Fragments arranged by size:
3202 2751 1021 849 754 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3061 1316
Size: 1745 6992
XbaI T'CTAG_A
Cuts at: 5818 6073 5818
Size: 255 8482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3007 5088 5623 5920 3007
Size: 2081 535 297 5824
Fragments arranged by size:
5824 2081 535 297
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8737
XmaI C'CCGG_G
Cuts at: 1870 4376 6062 1870
Size: 2506 1686 4545
Fragments arranged by size:
4545 2506 1686
XmnI GAAnn'nnTTC
Cuts at: 3113 3491 4927 6866 3113
Size: 378 1436 1939 4984
Fragments arranged by size:
4984 1939 1436 378
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6545 276
Size: 53 83 186 936 5011 2468
Fragments arranged by size:
5011 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BsiWI BstZ17I EcoRV FseI
FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SexAI SfiI SrfI SwaI