Restriction map for
pFN21AA0292
(Circular) (Six-base) MAPSORT of: pFN21AA0292.seq Check: 4574 from: 1 to: 10585
pFN21AA0292 10585 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8395 278
Size: 53 83 186 936 6859 2468
Fragments arranged by size:
6859 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 7928 1937
Size: 1059 4932 4594
Fragments arranged by size:
4932 4594 1059
Acc65I G'GTAC_C
Cuts at: 3331 7906 3331
Size: 4575 6010
AclI AA'CG_TT
Cuts at: 1563 8712 9085 1563
Size: 7149 373 3063
Fragments arranged by size:
7149 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3079 3528 3546 3557 4259 5859 6204 6288
Size: 1447 449 18 11 702 1600 345 84
Cuts at: 6288 6558 7482 8648 10032 10300 1632
Size: 270 924 1166 1384 268 1917
Fragments arranged by size:
1917 1600 1447 1384 1166 924 702 449
345 270 268 84 18 11
AfeI AGC'GCT
Cuts at: 7638 7638
Size: 10585
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10388
AflIII A'CryG_T
Cuts at: 1203 3420 9484 10461 1203
Size: 2217 6064 977 1327
Fragments arranged by size:
6064 2217 1327 977
AgeI A'CCGG_T
Cuts at: 9416 9416
Size: 10585
AhdI GACnn_n'nnGTC
Cuts at: 4631 9314 4631
Size: 4683 5902
AleI CACnn'nnGTG
Cuts at: 1166 4668 7266 1166
Size: 3502 2598 4485
Fragments arranged by size:
4485 3502 2598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3511 7284 1184
Size: 2327 3773 4485
Fragments arranged by size:
4485 3773 2327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5296 5328 5296
Size: 32 10553
AlwNI CAG_nnn'CTG
Cuts at: 2195 2198 2584 3356 4490 6368 6865 7442 8328
Size: 3 386 772 1134 1878 497 577 886
Cuts at: 8328 9900 2195
Size: 1572 2880
Fragments arranged by size:
2880 1878 1572 1134 886 772 577 497
386 3
ApaI G_GGCC'C
Cuts at: 4073 4460 6778 4073
Size: 387 2318 7880
Fragments arranged by size:
7880 2318 387
ApaLI G'TGCA_C
Cuts at: 8642 9798 8642
Size: 1156 9429
ApoI r'AATT_y
Cuts at: 1493 2122 2899 3234 3502 4247 5242 7556 7894
Size: 629 777 335 268 745 995 2314 338
Cuts at: 7894 8120 8272 10381 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2314 2109 1697 995 777 745 629 338
335 268 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9139 160
Size: 872 8107 1606
Fragments arranged by size:
8107 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10585
AvaI C'yCGr_G
Cuts at: 1870 1943 3831 5205 7910 1870
Size: 73 1888 1374 2705 4545
Fragments arranged by size:
4545 2705 1888 1374 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5255 5288 5255
Size: 33 10552
BamHI G'GATC_C
Cuts at: 7915 8282 7915
Size: 367 10218
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3331 6389 7107 7368 7685
Size: 325 217 627 1544 3058 718 261 317
Cuts at: 7685 7788 7906 9361 618
Size: 103 118 1455 1842
Fragments arranged by size:
3058 1842 1544 1455 718 627 325 317
261 217 118 103
BanII G_rGCy'C
Cuts at: 1391 4073 4460 4651 5212 5408 6778 7422 7904
Size: 2682 387 191 561 196 1370 644 482
Cuts at: 7904 1391
Size: 4072
Fragments arranged by size:
4072 2682 1370 644 561 482 387 196
191
BbeI G_GCGC'C
Cuts at: 7372 7689 7372
Size: 317 10268
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2706 3461 3815 4803 6294 6347 7086
Size: 971 807 755 354 988 1491 53 739
Cuts at: 7086 7389 928
Size: 303 4124
Fragments arranged by size:
4124 1491 988 971 807 755 739 354
303 53
BbvCI CC'TCA_GC
Cuts at: 2325 5676 6687 6960 7859 2325
Size: 3351 1011 273 899 5051
Fragments arranged by size:
5051 3351 1011 899 273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 1997 2021 2031 8776 8810 1903
Size: 34 50 10 24 10 6745 34 3678
Fragments arranged by size:
6745 3678 50 34 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 4194 8477 9687 4194
Size: 4283 1210 5092
Fragments arranged by size:
5092 4283 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10585
BfrBI ATG'CAT
Cuts at: 3953 3953
Size: 10585
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2341 4053 4969
Size: 107 122 71 631 605 669 1712 916
Cuts at: 4969 5480 6521 9196 136
Size: 511 1041 2675 1525
Fragments arranged by size:
2675 1712 1525 1041 916 669 631 605
511 122 107 71
BglII A'GATC_T
Cuts at: 10580 10580
Size: 10585
BlpI GC'TnA_GC
Cuts at: 8002 8002
Size: 10585
Bme1580I G_kGCm'C
Cuts at: 1790 4073 4460 5476 6778 7110 7491 7793 7820
Size: 2283 387 1016 1302 332 381 302 27
Cuts at: 7820 8646 9802 1790
Size: 826 1156 2573
Fragments arranged by size:
2573 2283 1302 1156 1016 826 387 381
332 302 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10585
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2484 3189 3276
Size: 416 53 83 399 24 1067 705 87
Cuts at: 3276 4462 4667 5463 5793 8316 9274 442
Size: 1186 205 796 330 2523 958 1753
Fragments arranged by size:
2523 1753 1186 1067 958 796 705 416
399 330 205 87 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2217 4404 1056
Size: 1161 2187 7237
Fragments arranged by size:
7237 2187 1161
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3766 6195 7795 3766
Size: 2429 1600 6556
Fragments arranged by size:
6556 2429 1600
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 2472 3102 3176 3559 3960 4866 4926
Size: 1044 189 630 74 383 401 906 60
Cuts at: 4926 5323 5577 5598 6483 7538 9245 1239
Size: 397 254 21 885 1055 1707 2579
Fragments arranged by size:
2579 1707 1055 1044 906 885 630 401
397 383 254 189 74 60 21
Bpu10I CC'TnA_GC
Cuts at: 2325 3762 5319 5676 6687 6960 7512 7794 7859
Size: 1437 1557 357 1011 273 552 282 65
Cuts at: 7859 2325
Size: 5051
Fragments arranged by size:
5051 1557 1437 1011 552 357 282 273
65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3372 4305 4803 8710 9575 9873 3372
Size: 933 498 3907 865 298 4084
Fragments arranged by size:
4084 3907 933 865 498 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2213 4437 4842 5082 6430 6622 9248
Size: 617 714 2224 405 240 1348 192 2626
Cuts at: 9248 882
Size: 2219
Fragments arranged by size:
2626 2224 2219 1348 714 617 405 240
192
BsaAI yAC'GTr
Cuts at: 493 1204 6280 8376 493
Size: 711 5076 2096 2702
Fragments arranged by size:
5076 2702 2096 711
BsaBI GATnn'nnATC
Cuts at: 10579 10579
Size: 10585
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7369 7686 8392 8774
Size: 53 83 186 936 5836 317 706 382
Cuts at: 8774 9409 275
Size: 635 1451
Fragments arranged by size:
5836 1451 936 706 635 382 317 186
83 53
BsaWI w'CCGG_w
Cuts at: 5136 7146 9018 9416 9690 9837 10248 5136
Size: 2010 1872 398 274 147 411 5473
Fragments arranged by size:
5473 2010 1872 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2389 2419 3148 3178 5296
Size: 30 165 30 986 30 729 30 2118
Cuts at: 5296 5326 6126 6156 1178
Size: 30 800 30 5607
Fragments arranged by size:
5607 2118 986 800 729 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2199 2313 2716 2719 2722 2725 2728 3156
Size: 1013 114 403 3 3 3 3 428
Cuts at: 3156 3435 3505 3667 3996 3999 5303 5779 6115
Size: 279 70 162 329 3 1304 476 336
Cuts at: 6115 7313 7316 7784 1186
Size: 1198 3 468 3987
Fragments arranged by size:
3987 1304 1198 1013 476 468 428 403
336 329 279 162 114 70 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 3963 5483 6128 6777 6858 9788 10284 3963
Size: 1520 645 649 81 2930 496 4264
Fragments arranged by size:
4264 2930 1520 649 645 496 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 3481 1163
Size: 573 633 1112 8267
Fragments arranged by size:
8267 1112 633 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8035 8796 9824 1540
Size: 275 186 6034 761 1028 2301
Fragments arranged by size:
6034 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2500 3379 3629 3697 8646 8731 9802 2500
Size: 879 250 68 4949 85 1071 3283
Fragments arranged by size:
4949 3283 1071 879 250 85 68
BsmI GAATG_Cn'
Cuts at: 2809 5888 8096 8189 2809
Size: 3079 2208 93 5205
Fragments arranged by size:
5205 3079 2208 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4706 4706
Size: 10585
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2500 3379 3629 3697 4073 4460 4651
Size: 399 710 879 250 68 376 387 191
Cuts at: 4651 5212 5408 5476 6778 7110 7422 7491 7793
Size: 561 196 68 1302 332 312 69 302
Cuts at: 7793 7820 7904 8646 8731 9802 1391
Size: 27 84 742 85 1071 2174
Fragments arranged by size:
2174 1302 1071 879 742 710 561 399
387 376 332 312 302 250 196 191
85 84 69 68 68 27
BspEI T'CCGG_A
Cuts at: 10248 10248
Size: 10585
BspHI T'CATG_A
Cuts at: 5032 5760 8474 10204 5032
Size: 728 2714 1730 5413
Fragments arranged by size:
5413 2714 1730 728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5225 7940 844
Size: 4381 2715 3489
Fragments arranged by size:
4381 3489 2715
BsrBI CCG'CTC
Cuts at: 1394 1823 4779 7377 7879 8472 1394
Size: 429 2956 2598 502 593 3507
Fragments arranged by size:
3507 2956 2598 593 502 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3931 4900 7558 9080 9254 65
Size: 1183 2683 969 2658 1522 174 1396
Fragments arranged by size:
2683 2658 1522 1396 1183 969 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3682 9229 9416 1700
Size: 110 1872 5547 187 2869
Fragments arranged by size:
5547 2869 1872 187 110
BsrGI T'GTAC_A
Cuts at: 96 6944 96
Size: 6848 3737
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10585
BssSI C'ACGA_G
Cuts at: 8645 9657 8645
Size: 1012 9573
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2195 2198 2989 3692 4270 794
Size: 1401 3 791 703 578 7109
Fragments arranged by size:
7109 1401 791 703 578 3
BstBI TT'CG_AA
Cuts at: 6113 9389 6113
Size: 3276 7309
BstEII G'GTnAC_C
Cuts at: 1184 2237 2672 4280 7157 1184
Size: 1053 435 1608 2877 4612
Fragments arranged by size:
4612 2877 1608 1053 435
BstXI CCAn_nnnn'nTGG
Cuts at: 2126 4375 5483 2126
Size: 2249 1108 7228
Fragments arranged by size:
7228 2249 1108
BstYI r'GATC_y
Cuts at: 1972 2810 3352 5572 7915 8282 8667 8684 10136
Size: 838 542 2220 2343 367 385 17 1452
Cuts at: 10136 10222 10234 10580 1972
Size: 86 12 346 1977
Fragments arranged by size:
2343 2220 1977 1452 838 542 385 367
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 2997 2997
Size: 10585
Bsu36I CC'TnA_GG
Cuts at: 3054 6519 3054
Size: 3465 7120
BtgI C'CryG_G
Cuts at: 513 1065 2035 4219 4752 4888 5360 6035 513
Size: 552 970 2184 533 136 472 675 5063
Fragments arranged by size:
5063 2184 970 675 552 533 472 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2222 487
Size: 683 56 996 8850
Fragments arranged by size:
8850 996 683 56
BtsI GCAGTG_nn'
Cuts at: 2161 2167 2173 3214 4852 7459 7669 8101 8895
Size: 6 6 1041 1638 2607 210 432 794
Cuts at: 8895 8915 2161
Size: 20 3831
Fragments arranged by size:
3831 2607 1638 1041 794 432 210 20
6 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10585
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10550
DraI TTT'AAA
Cuts at: 7889 7889
Size: 10585
DraIII CAC_nnn'GTG
Cuts at: 2833 3692 2833
Size: 859 9726
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9592 809
Size: 507 8276 1802
Fragments arranged by size:
8276 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2227 2599 2698
Size: 54 1425 50 275 18 397 372 99
Cuts at: 2698 3393 3619 4045 4054 4216 7344 8032 8921
Size: 695 226 426 9 162 3128 688 889
Cuts at: 8921 10550 8
Size: 1629 43
Fragments arranged by size:
3128 1629 1425 889 695 688 426 397
372 275 226 162 99 54 50 43
18 9
EagI C'GGCC_G
Cuts at: 1537 1812 8032 1537
Size: 275 6220 4090
Fragments arranged by size:
6220 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 3735 5360 6164 6218 6297 6303 6534
Size: 729 1653 1625 804 54 79 6 231
Cuts at: 6534 7302 8515 1353
Size: 768 1213 3423
Fragments arranged by size:
3423 1653 1625 1213 804 768 729 231
79 54 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5083 9172 9546 9692 387
Size: 1064 138 3494 4089 374 146 1280
Fragments arranged by size:
4089 3494 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2472 3079 3102 3176 3528 3546
Size: 393 651 189 607 23 74 352 18
Cuts at: 3546 3557 3559 3960 4259 4866 4926 5323 5577
Size: 11 2 401 299 607 60 397 254
Cuts at: 5577 5598 5859 6204 6288 6483 6558 7482 7538
Size: 21 261 345 84 195 75 924 56
Cuts at: 7538 8648 9245 10032 10300 1239
Size: 1110 597 787 268 1524
Fragments arranged by size:
1524 1110 924 787 651 607 607 597
401 397 393 352 345 299 268 261
254 195 189 84 75 74 60 56
23 21 18 11 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2588 3463 3978 4262 4465 5232 6183 2588
Size: 875 515 284 203 767 951 6990
Fragments arranged by size:
6990 951 875 767 515 284 203
EcoO109I rG'GnC_Cy
Cuts at: 2928 3069 3468 4070 4408 4456 4457 4639 4975
Size: 141 399 602 338 48 1 182 336
Cuts at: 4975 5224 5959 6774 7076 7397 2928
Size: 249 735 815 302 321 6116
Fragments arranged by size:
6116 815 735 602 399 338 336 321
302 249 182 141 48 1
EcoRI G'AATT_C
Cuts at: 5242 7894 8272 5242
Size: 2652 378 7555
Fragments arranged by size:
7555 2652 378
EcoRV GAT'ATC
Cuts at: 5926 5926
Size: 10585
FspI TGC'GCA
Cuts at: 9091 9091
Size: 10585
HaeII r_GCGC'y
Cuts at: 1678 3762 5491 5880 7049 7372 7640 7689 9732
Size: 2084 1729 389 1169 323 268 49 2043
Cuts at: 9732 1678
Size: 2531
Fragments arranged by size:
2531 2084 2043 1729 1169 389 323 268
49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2389 2421 3268 3300 3866
Size: 32 135 32 817 32 847 32 566
Cuts at: 3866 3898 4004 4036 4504 4536 4538 4570 4587
Size: 32 106 32 468 32 2 32 17
Cuts at: 4587 4617 4619 4649 5915 5947 6146 6178 9226
Size: 30 2 30 1266 32 199 32 3048
Cuts at: 9226 9258 9300 9332 1373
Size: 32 42 32 2626
Fragments arranged by size:
3048 2626 1266 847 817 566 468 199
135 106 42 32 32 32 32 32
32 32 32 32 32 32 32 30
30 17 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 7929 8175 669
Size: 988 281 5991 246 3079
Fragments arranged by size:
5991 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10585
HpaI GTT'AAC
Cuts at: 8175 8175
Size: 10585
KasI G'GCGC_C
Cuts at: 7368 7685 7368
Size: 317 10268
KpnI G_GTAC'C
Cuts at: 3335 7910 3335
Size: 4575 6010
MfeI C'AATT_G
Cuts at: 8184 8184
Size: 10585
MluI A'CGCG_T
Cuts at: 10461 10461
Size: 10585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3287 3807 4047 4733 7854 9699 9883 1305
Size: 1982 520 240 686 3121 1845 184 2007
Fragments arranged by size:
3121 2007 1982 1845 686 520 240 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2229 2601 2700 3395 3621
Size: 54 1425 343 397 372 99 695 226
Cuts at: 3621 4047 4056 4218 10
Size: 426 9 162 6377
Fragments arranged by size:
6377 1425 695 426 397 372 343 226
162 99 54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 4668 4964 5217 7235 7266 8543 8902
Size: 648 3502 296 253 2018 31 1277 359
Cuts at: 8902 9061 518
Size: 159 2042
Fragments arranged by size:
3502 2042 2018 1277 648 359 296 253
159 31
MspA1I CmG'CkG
Cuts at: 1606 2195 2641 3122 3535 4067 4890 5251 5310
Size: 589 446 481 413 532 823 361 59
Cuts at: 5310 5607 8001 8678 9826 10071 1606
Size: 297 2394 677 1148 245 2120
Fragments arranged by size:
2394 2120 1148 823 677 589 532 481
446 413 361 297 245 59
NarI GG'CG_CC
Cuts at: 7369 7686 7369
Size: 317 10268
NcoI C'CATG_G
Cuts at: 513 1065 4219 4752 513
Size: 552 3154 533 6346
Fragments arranged by size:
6346 3154 552 533
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10585
NheI G'CTAG_C
Cuts at: 1052 2213 4400 1052
Size: 1161 2187 7237
Fragments arranged by size:
7237 2187 1161
NotI GC'GGCC_GC
Cuts at: 8032 8032
Size: 10585
NsiI A_TGCA'T
Cuts at: 3955 3955
Size: 10585
NspI r_CATG'y
Cuts at: 1132 1229 2987 3424 3772 6356 7943 9488 1132
Size: 97 1758 437 348 2584 1587 1545 2229
Fragments arranged by size:
2584 2229 1758 1587 1545 437 348 97
PasI CC'CwG_GG
Cuts at: 1348 2368 4461 4643 5081 6451 1348
Size: 1020 2093 182 438 1370 5482
Fragments arranged by size:
5482 2093 1370 1020 438 182
PciI A'CATG_T
Cuts at: 3420 9484 3420
Size: 6064 4521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6865 1108
Size: 5757 4828
PfoI T'CCnGG_A
Cuts at: 3976 4108 5614 3976
Size: 132 1506 8947
Fragments arranged by size:
8947 1506 132
PmeI GTTT'AAAC
Cuts at: 7889 7889
Size: 10585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5296 5328 8654 8686 10167 10199 5296
Size: 32 3326 32 1481 32 5682
Fragments arranged by size:
5682 3326 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2928 3069 4639 4975 5224 5959 7076 2928
Size: 141 1570 336 249 735 1117 6437
Fragments arranged by size:
6437 1570 1117 735 336 249 141
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10585
PsiI TTA'TAA
Cuts at: 5259 8155 5259
Size: 2896 7689
PspOMI G'GGCC_C
Cuts at: 4069 4456 6774 4069
Size: 387 2318 7880
Fragments arranged by size:
7880 2318 387
PstI C_TGCA'G
Cuts at: 830 3304 3904 4066 4266 5338 6718 7074 7632
Size: 2474 600 162 200 1072 1380 356 558
Cuts at: 7632 7937 830
Size: 305 3478
Fragments arranged by size:
3478 2474 1380 1072 600 558 356 305
200 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10585
PvuII CAG'CTG
Cuts at: 2195 3122 3535 5251 2195
Size: 927 413 1716 7529
Fragments arranged by size:
7529 1716 927 413
SacII CC_GC'GG
Cuts at: 4891 4891
Size: 10585
SalI G'TCGA_C
Cuts at: 1936 7927 1936
Size: 5991 4594
SanDI GG'GwC_CC
Cuts at: 2928 4639 4975 5224 2928
Size: 1711 336 249 8289
Fragments arranged by size:
8289 1711 336 249
SapI GCTCTTCn'nnn_
Cuts at: 7302 7302
Size: 10585
SbfI CC_TGCA'GG
Cuts at: 4266 7632 7937 4266
Size: 3366 305 6914
Fragments arranged by size:
6914 3366 305
ScaI AGT'ACT
Cuts at: 3385 4318 8833 10545 3385
Size: 933 4515 1712 3425
Fragments arranged by size:
4515 3425 1712 933
SexAI A'CCwGG_T
Cuts at: 5698 7110 7602 5698
Size: 1412 492 8681
Fragments arranged by size:
8681 1412 492
SfcI C'TryA_G
Cuts at: 826 1045 2304 2627 3148 3300 3900 4062 4123
Size: 219 1259 323 521 152 600 162 61
Cuts at: 4123 4188 4262 5334 6066 6714 7070 7628 7933
Size: 65 74 1072 732 648 356 558 305
Cuts at: 7933 9068 9749 9940 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1259 1135 1072 732 681 648 600
558 521 356 323 305 219 191 162
152 74 65 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 2341 4053 2341
Size: 1712 8873
SfoI GGC'GCC
Cuts at: 7370 7687 7370
Size: 317 10268
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10585
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10585
SmaI CCC'GGG
Cuts at: 1872 3833 7912 1872
Size: 1961 4079 4545
Fragments arranged by size:
4545 4079 1961
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3387 4320 4818 8689 9590 9852
Size: 197 926 1444 933 498 3871 901 262
Cuts at: 9852 820
Size: 1553
Fragments arranged by size:
3871 1553 1444 933 926 901 498 262
197
SnaBI TAC'GTA
Cuts at: 493 8376 493
Size: 7883 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10585
SphI G_CATG'C
Cuts at: 1132 7943 1132
Size: 6811 3774
SspI AAT'ATT
Cuts at: 5 52 6094 8509 5
Size: 47 6042 2415 2081
Fragments arranged by size:
6042 2415 2081 47
StuI AGG'CCT
Cuts at: 1517 2335 1517
Size: 818 9767
StyI C'CwwG_G
Cuts at: 513 1065 2440 4219 4752 5372 5955 6296 6430
Size: 552 1375 1779 533 620 583 341 134
Cuts at: 6430 6531 7692 8024 513
Size: 101 1161 332 3074
Fragments arranged by size:
3074 1779 1375 1161 620 583 552 533
341 332 134 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4714 5004 6443 6872 7145 7392 8612
Size: 1255 3134 290 1439 429 273 247 1220
Cuts at: 8612 8629 8782 8967 9412 325
Size: 17 153 185 445 1498
Fragments arranged by size:
3134 1498 1439 1255 1220 445 429 290
273 247 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3042 3383 4316
Size: 274 80 33 51 1443 1065 341 933
Cuts at: 4316 6944 8831 10543 96
Size: 2628 1887 1712 138
Fragments arranged by size:
2628 1887 1712 1443 1065 933 341 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2060 2092 2817 2849 7068 7100 7141 7173 10545
Size: 32 725 32 4219 32 41 32 3372
Cuts at: 10545 10577 2060
Size: 32 2068
Fragments arranged by size:
4219 3372 2068 725 41 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6251 7090 1316
Size: 4935 839 4811
Fragments arranged by size:
4935 4811 839
XbaI T'CTAG_A
Cuts at: 7921 7921
Size: 10585
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5657 6862 5657
Size: 1205 9380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10585
XmaI C'CCGG_G
Cuts at: 1870 3831 7910 1870
Size: 1961 4079 4545
Fragments arranged by size:
4545 4079 1961
XmnI GAAnn'nnTTC
Cuts at: 2335 4272 8714 2335
Size: 1937 4442 4206
Fragments arranged by size:
4442 4206 1937
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8393 276
Size: 53 83 186 936 6859 2468
Fragments arranged by size:
6859 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BsiWI EcoICRI FalI FseI FspAI
NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII
SacI SrfI SwaI