Restriction map for
pFN21AA0291
(Circular) (Six-base) MAPSORT of: pFN21AA0291.seq Check: 5041 from: 1 to: 7738
pFN21AA0291 7738 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4906 4906
Size: 7738
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5548 278
Size: 53 83 186 936 4012 2468
Fragments arranged by size:
4012 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5081 1937
Size: 3144 4594
Acc65I G'GTAC_C
Cuts at: 5059 5059
Size: 7738
AclI AA'CG_TT
Cuts at: 1563 5865 6238 1563
Size: 4302 373 3063
Fragments arranged by size:
4302 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 3107 3632 3704 3866 4532 5801 7185
Size: 413 1062 525 72 162 666 1269 1384
Cuts at: 7185 7453 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1269 1062 666 525 413 268
162 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7541
AflIII A'CryG_T
Cuts at: 1203 6637 7614 1203
Size: 5434 977 1327
Fragments arranged by size:
5434 1327 977
AgeI A'CCGG_T
Cuts at: 6569 6569
Size: 7738
AhdI GACnn_n'nnGTC
Cuts at: 2943 6467 2943
Size: 3524 4214
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7738
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3035 3240 1184
Size: 1851 205 5682
Fragments arranged by size:
5682 1851 205
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 7706
AlwNI CAG_nnn'CTG
Cuts at: 2180 2296 2509 3118 3475 4603 5481 7053 2180
Size: 116 213 609 357 1128 878 1572 2865
Fragments arranged by size:
2865 1572 1128 878 609 357 213 116
ApaI G_GGCC'C
Cuts at: 2201 3815 4015 4453 2201
Size: 1614 200 438 5486
Fragments arranged by size:
5486 1614 438 200
ApaLI G'TGCA_C
Cuts at: 4094 5795 6951 4094
Size: 1701 1156 4881
Fragments arranged by size:
4881 1701 1156
ApoI r'AATT_y
Cuts at: 1493 5047 5273 5425 7534 1493
Size: 3554 226 152 2109 1697
Fragments arranged by size:
3554 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6292 160
Size: 872 5260 1606
Fragments arranged by size:
5260 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7738
AvaI C'yCGr_G
Cuts at: 1870 1943 2186 2464 3058 3484 3808 4573 4841
Size: 73 243 278 594 426 324 765 268
Cuts at: 4841 5063 1870
Size: 222 4545
Fragments arranged by size:
4545 765 594 426 324 278 268 243
222 73
BamHI G'GATC_C
Cuts at: 2612 5068 5435 2612
Size: 2456 367 4915
Fragments arranged by size:
4915 2456 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2801 3062 3818 4524 4837
Size: 325 217 627 1014 261 756 706 313
Cuts at: 4837 5059 6514 618
Size: 222 1455 1842
Fragments arranged by size:
1842 1455 1014 756 706 627 325 313
261 222 217
BanII G_rGCy'C
Cuts at: 1391 2201 2465 2772 3256 3435 3465 3815 4015
Size: 810 264 307 484 179 30 350 200
Cuts at: 4015 4453 5057 1391
Size: 438 604 4072
Fragments arranged by size:
4072 810 604 484 438 350 307 264
200 179 30
BbeI G_GCGC'C
Cuts at: 3822 4528 4841 3822
Size: 706 313 6719
Fragments arranged by size:
6719 706 313
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2600 2702 2909 4565 928
Size: 971 701 102 207 1656 4101
Fragments arranged by size:
4101 1656 971 701 207 102
BbvCI CC'TCA_GC
Cuts at: 3144 3144
Size: 7738
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5929 5963 1903
Size: 34 50 34 3908 34 3678
Fragments arranged by size:
3908 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5630 6840 5630
Size: 1210 6528
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7738
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2064 3015 3614
Size: 107 122 71 631 605 392 951 599
Cuts at: 3614 3623 6349 136
Size: 9 2726 1525
Fragments arranged by size:
2726 1525 951 631 605 599 392 122
107 71 9
BglII A'GATC_T
Cuts at: 3054 4164 7733 3054
Size: 1110 3569 3059
Fragments arranged by size:
3569 3059 1110
BlpI GC'TnA_GC
Cuts at: 4911 5155 4911
Size: 244 7494
Bme1580I G_kGCm'C
Cuts at: 1790 2201 2402 2528 2804 2822 3065 3815 4015
Size: 411 201 126 276 18 243 750 200
Cuts at: 4015 4098 4453 5799 6955 1790
Size: 83 355 1346 1156 2573
Fragments arranged by size:
2573 1346 1156 750 411 355 276 243
201 200 126 83 18
BmgBI CAC'GTC
Cuts at: 1318 4117 1318
Size: 2799 4939
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2088 5469 6427
Size: 416 53 83 399 24 671 3381 958
Cuts at: 6427 442
Size: 1753
Fragments arranged by size:
3381 1753 958 671 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2115 1056
Size: 1059 6679
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4183 4183
Size: 7738
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2038 2393 3329 3401 3443 3449 3803 3905
Size: 799 355 936 72 42 6 354 102
Cuts at: 3905 3938 4040 4067 4265 4487 4550 4562 4670
Size: 33 102 27 198 222 63 12 108
Cuts at: 4670 6398 1239
Size: 1728 2579
Fragments arranged by size:
2579 1728 936 799 355 354 222 198
108 102 102 72 63 42 33 27
12 6
Bpu10I CC'TnA_GC
Cuts at: 3144 4445 4688 3144
Size: 1301 243 6194
Fragments arranged by size:
6194 1301 243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3185 4139 4721 5863 6728 7026 3185
Size: 954 582 1142 865 298 3897
Fragments arranged by size:
3897 1142 954 865 582 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2840 3023 3123 3369 3914 4538 6401
Size: 617 1341 183 100 246 545 624 1863
Cuts at: 6401 882
Size: 2219
Fragments arranged by size:
2219 1863 1341 624 617 545 246 183
100
BsaAI yAC'GTr
Cuts at: 493 1204 2716 3934 5529 493
Size: 711 1512 1218 1595 2702
Fragments arranged by size:
2702 1595 1512 1218 711
BsaBI GATnn'nnATC
Cuts at: 2864 7732 2864
Size: 4868 2870
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3513 3819 4503 4525
Size: 53 83 186 936 1980 306 684 22
Cuts at: 4525 4569 4838 4884 5545 5927 6562 275
Size: 44 269 46 661 382 635 1451
Fragments arranged by size:
1980 1451 936 684 661 635 382 306
269 186 83 53 46 44 22
BsaWI w'CCGG_w
Cuts at: 3499 4979 6171 6569 6843 6990 7401 3499
Size: 1480 1192 398 274 147 411 3836
Fragments arranged by size:
3836 1480 1192 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3344 3374 4460 4490 4535
Size: 30 165 30 1941 30 1086 30 45
Cuts at: 4535 4565 1178
Size: 30 4351
Fragments arranged by size:
4351 1941 1086 165 45 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2149 2152 2977 3251 3326 3329 3334 3368
Size: 963 3 825 274 75 3 5 34
Cuts at: 3368 3371 3440 3443 3473 3995 4294 4361 4957
Size: 3 69 3 30 522 299 67 596
Cuts at: 4957 1186
Size: 3967
Fragments arranged by size:
3967 963 825 596 522 299 274 75
69 67 34 30 5 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2015 2887 2948 3005 3822 3889 3989 4014 4308
Size: 872 61 57 817 67 100 25 294
Cuts at: 4308 6941 7437 2015
Size: 2633 496 2316
Fragments arranged by size:
2633 2316 872 817 496 294 100 67
61 57 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2120 2309 1163
Size: 573 384 189 6592
Fragments arranged by size:
6592 573 384 189
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 2172 2457 4290 5188 5949
Size: 275 186 37 134 285 1833 898 761
Cuts at: 5949 6977 1540
Size: 1028 2301
Fragments arranged by size:
2301 1833 1028 898 761 285 275 186
134 37
BsiHKAI G_wGCw'C
Cuts at: 3435 3465 3879 4098 4304 4877 5799 5884 6955
Size: 30 414 219 206 573 922 85 1071
Cuts at: 6955 3435
Size: 4218
Fragments arranged by size:
4218 1071 922 573 414 219 206 85
30
BsmI GAATG_Cn'
Cuts at: 2518 5249 5342 2518
Size: 2731 93 4914
Fragments arranged by size:
4914 2731 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2297 4343 2297
Size: 2046 5692
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2402 2465 2528 2772 2804 2822
Size: 399 411 201 63 63 244 32 18
Cuts at: 2822 3065 3256 3435 3465 3815 3879 4015 4098
Size: 243 191 179 30 350 64 136 83
Cuts at: 4098 4304 4453 4877 5057 5799 5884 6955 1391
Size: 206 149 424 180 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 424 411 399 350 244
243 206 201 191 180 179 149 136
85 83 64 63 63 32 30 18
BspEI T'CCGG_A
Cuts at: 3499 7401 3499
Size: 3902 3836
BspHI T'CATG_A
Cuts at: 5627 7357 5627
Size: 1730 6008
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2667 4906 5093 844
Size: 1823 2239 187 3489
Fragments arranged by size:
3489 2239 1823 187
BsrBI CCG'CTC
Cuts at: 1394 1823 2254 3541 4201 4618 5625 1394
Size: 429 431 1287 660 417 1007 3507
Fragments arranged by size:
3507 1287 1007 660 431 429 417
BsrDI GCAATG_nn'
Cuts at: 65 1248 2513 6233 6407 65
Size: 1183 1265 3720 174 1396
Fragments arranged by size:
3720 1396 1265 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2597 4041 6382 6569 1700
Size: 110 787 1444 2341 187 2869
Fragments arranged by size:
2869 2341 1444 787 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7738
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7738
BssSI C'ACGA_G
Cuts at: 3263 4097 5798 6810 3263
Size: 834 1701 1012 4191
Fragments arranged by size:
4191 1701 1012 834
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2180 794
Size: 1386 6352
BstBI TT'CG_AA
Cuts at: 6542 6542
Size: 7738
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7738
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2330 2914 3307 2062
Size: 268 584 393 6493
Fragments arranged by size:
6493 584 393 268
BstYI r'GATC_y
Cuts at: 1972 2612 3054 3334 3522 4164 5068 5435 5820
Size: 640 442 280 188 642 904 367 385
Cuts at: 5820 5837 7289 7375 7387 7733 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 904 642 640 442 385 367
346 280 188 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2038 2061 2451 2484 2919 2991 3066
Size: 552 973 23 390 33 435 72 75
Cuts at: 3066 513
Size: 5185
Fragments arranged by size:
5185 973 552 435 390 75 72 33
23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6999
Fragments arranged by size:
6999 683 56
BtsI GCAGTG_nn'
Cuts at: 2132 3019 3476 3858 5254 6048 6068 2132
Size: 887 457 382 1396 794 20 3802
Fragments arranged by size:
3802 1396 887 794 457 382 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7738
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7703
DraI TTT'AAA
Cuts at: 5042 5042
Size: 7738
DraIII CAC_nnn'GTG
Cuts at: 2485 2857 2485
Size: 372 7366
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6745 809
Size: 507 5429 1802
Fragments arranged by size:
5429 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2106 2169
Size: 54 1425 50 275 18 205 71 63
Cuts at: 2169 2454 2529 2916 3150 3396 3552 3648 4173
Size: 285 75 387 234 246 156 96 525
Cuts at: 4173 5185 6074 7703 8
Size: 1012 889 1629 43
Fragments arranged by size:
1629 1425 1012 889 525 387 285 275
246 234 205 156 96 75 71 63
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2035 2169 2454 5185 1537
Size: 275 223 134 285 2731 4090
Fragments arranged by size:
4090 2731 285 275 223 134
EarI CTCTTCn'nnn_
Cuts at: 1353 2843 3040 5668 1353
Size: 1490 197 2628 3423
Fragments arranged by size:
3423 2628 1490 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2225 3641 4732 6325 6699 6845
Size: 1064 138 636 1416 1091 1593 374 146
Cuts at: 6845 387
Size: 1280
Fragments arranged by size:
1593 1416 1280 1091 1064 636 374 146
138
EcoICRI GAG'CTC
Cuts at: 3433 3463 3433
Size: 30 7708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2045 2393 3107 3329 3401 3443
Size: 393 406 7 348 714 222 72 42
Cuts at: 3443 3449 3632 3704 3803 3866 3905 3938 4040
Size: 6 183 72 99 63 39 33 102
Cuts at: 4040 4067 4265 4487 4532 4550 4562 4670 5801
Size: 27 198 222 45 18 12 108 1131
Cuts at: 5801 6398 7185 7453 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1131 787 714 597 406 393 348
268 222 222 198 183 108 102 99
72 72 63 45 42 39 33 27
18 12 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3200 4511 3200
Size: 1311 6427
EcoO109I rG'GnC_Cy
Cuts at: 2198 2656 3488 3607 3787 3949 4294 4378 4449
Size: 458 832 119 180 162 345 84 71
Cuts at: 4449 4684 4753 2198
Size: 235 69 5183
Fragments arranged by size:
5183 832 458 345 235 180 162 119
84 71 69
EcoRI G'AATT_C
Cuts at: 5047 5425 5047
Size: 378 7360
FspI TGC'GCA
Cuts at: 6244 6244
Size: 7738
HaeII r_GCGC'y
Cuts at: 1678 3822 4075 4528 4813 4841 6885 1678
Size: 2144 253 453 285 28 2044 2531
Fragments arranged by size:
2531 2144 2044 453 285 253 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2465 2497 2597 2604 2618
Size: 32 135 32 893 32 100 7 14
Cuts at: 2618 2629 2636 2650 2929 2961 3344 3376 3438
Size: 11 7 14 279 32 383 32 62
Cuts at: 3438 3470 4535 4567 4817 4849 6379 6411 6453
Size: 32 1065 32 250 32 1530 32 42
Cuts at: 6453 6485 1373
Size: 32 2626
Fragments arranged by size:
2626 1530 1065 893 383 279 250 135
100 62 42 32 32 32 32 32
32 32 32 32 32 14 14 11
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 5082 5328 669
Size: 988 281 3144 246 3079
Fragments arranged by size:
3144 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7738
HpaI GTT'AAC
Cuts at: 5328 5328
Size: 7738
KasI G'GCGC_C
Cuts at: 3818 4524 4837 3818
Size: 706 313 6719
Fragments arranged by size:
6719 706 313
KpnI G_GTAC'C
Cuts at: 5063 5063
Size: 7738
MfeI C'AATT_G
Cuts at: 5337 5337
Size: 7738
MluI A'CGCG_T
Cuts at: 7614 7614
Size: 7738
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2640 3324 4170 4779 6852 7036 1305
Size: 1335 684 846 609 2073 184 2007
Fragments arranged by size:
2073 2007 1335 846 684 609 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2918 3152 10
Size: 54 1425 343 1086 234 4596
Fragments arranged by size:
4596 1425 1086 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2924 3869 3932 4319 5696 6055 6214
Size: 648 1758 945 63 387 1377 359 159
Cuts at: 6214 518
Size: 2042
Fragments arranged by size:
2042 1758 1377 945 648 387 359 159
63
MspA1I CmG'CkG
Cuts at: 1606 2019 2177 2542 2631 3115 3307 3421 3883
Size: 413 158 365 89 484 192 114 462
Cuts at: 3883 4000 4018 4210 4867 5154 5831 6979 7224
Size: 117 18 192 657 287 677 1148 245
Cuts at: 7224 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 657 484 462 413 365
287 245 192 192 158 117 114 89
18
NarI GG'CG_CC
Cuts at: 3819 4525 4838 3819
Size: 706 313 6719
Fragments arranged by size:
6719 706 313
NcoI C'CATG_G
Cuts at: 513 1065 2061 2919 513
Size: 552 996 858 5332
Fragments arranged by size:
5332 996 858 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7738
NheI G'CTAG_C
Cuts at: 1052 2111 1052
Size: 1059 6679
NotI GC'GGCC_GC
Cuts at: 5185 5185
Size: 7738
NspI r_CATG'y
Cuts at: 1132 1229 5096 6641 1132
Size: 97 3867 1545 2229
Fragments arranged by size:
3867 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4222 4670 1348
Size: 2874 448 4416
Fragments arranged by size:
4416 2874 448
PciI A'CATG_T
Cuts at: 6637 6637
Size: 7738
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2485 1108
Size: 1377 6361
PfoI T'CCnGG_A
Cuts at: 4553 4553
Size: 7738
PmeI GTTT'AAAC
Cuts at: 5042 5042
Size: 7738
PmlI CAC'GTG
Cuts at: 3934 3934
Size: 7738
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2294 2326 5807 5839 7320 7352 2294
Size: 32 3481 32 1481 32 2680
Fragments arranged by size:
3481 2680 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2656 3488 3787 4684 2656
Size: 832 299 897 5710
Fragments arranged by size:
5710 897 832 299
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7738
PsiI TTA'TAA
Cuts at: 5308 5308
Size: 7738
PspOMI G'GGCC_C
Cuts at: 2197 3811 4011 4449 2197
Size: 1614 200 438 5486
Fragments arranged by size:
5486 1614 438 200
PstI C_TGCA'G
Cuts at: 830 3249 3342 3420 3969 3999 4056 4209 4386
Size: 2419 93 78 549 30 57 153 177
Cuts at: 4386 4860 5090 830
Size: 474 230 3478
Fragments arranged by size:
3478 2419 549 474 230 177 153 93
78 57 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7738
PvuII CAG'CTG
Cuts at: 2177 3115 3307 3421 3883 4018 4867 2177
Size: 938 192 114 462 135 849 5048
Fragments arranged by size:
5048 938 849 462 192 135 114
SacI G_AGCT'C
Cuts at: 3435 3465 3435
Size: 30 7708
SalI G'TCGA_C
Cuts at: 1936 5080 1936
Size: 3144 4594
SanDI GG'GwC_CC
Cuts at: 3488 3488
Size: 7738
SbfI CC_TGCA'GG
Cuts at: 4386 5090 4386
Size: 704 7034
ScaI AGT'ACT
Cuts at: 5986 7698 5986
Size: 1712 6026
SexAI A'CCwGG_T
Cuts at: 2804 2804
Size: 7738
SfcI C'TryA_G
Cuts at: 826 1045 3245 3338 3416 3965 3995 4052 4205
Size: 219 2200 93 78 549 30 57 153
Cuts at: 4205 4382 4856 5086 6221 6902 7093 826
Size: 177 474 230 1135 681 191 1471
Fragments arranged by size:
2200 1471 1135 681 549 474 230 219
191 177 153 93 78 57 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 3614 3614
Size: 7738
SfoI GGC'GCC
Cuts at: 3820 4526 4839 3820
Size: 706 313 6719
Fragments arranged by size:
6719 706 313
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7738
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7738
SmaI CCC'GGG
Cuts at: 1872 5065 1872
Size: 3193 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3200 4154 4736 5842 6743 7005
Size: 197 926 1257 954 582 1106 901 262
Cuts at: 7005 820
Size: 1553
Fragments arranged by size:
1553 1257 1106 954 926 901 582 262
197
SnaBI TAC'GTA
Cuts at: 493 5529 493
Size: 5036 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7738
SphI G_CATG'C
Cuts at: 1132 5096 1132
Size: 3964 3774
SspI AAT'ATT
Cuts at: 5 52 5662 5
Size: 47 5610 2081
Fragments arranged by size:
5610 2081 47
StuI AGG'CCT
Cuts at: 1517 3617 1517
Size: 2100 5638
StyI C'CwwG_G
Cuts at: 513 1065 2061 2118 2202 2736 2773 2892 2919
Size: 552 996 57 84 534 37 119 27
Cuts at: 2919 3153 3369 5177 513
Size: 234 216 1808 3074
Fragments arranged by size:
3074 1808 996 552 534 234 216 119
84 57 37 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2251 2839 2912 3522 4331 4451 5765
Size: 1255 671 588 73 610 809 120 1314
Cuts at: 5765 5782 5935 6120 6565 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1314 1255 809 671 610 588 445
185 153 120 73 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5984 7696 96
Size: 274 80 33 51 1443 4007 1712 138
Fragments arranged by size:
4007 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3761 3793 4460 4492 7698 7730 3761
Size: 32 667 32 3206 32 3769
Fragments arranged by size:
3769 3206 667 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4549 1316
Size: 3233 4505
XbaI T'CTAG_A
Cuts at: 5074 5074
Size: 7738
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3376 3575 3715 3913 3376
Size: 199 140 198 7201
Fragments arranged by size:
7201 199 198 140
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7738
XmaI C'CCGG_G
Cuts at: 1870 5063 1870
Size: 3193 4545
XmnI GAAnn'nnTTC
Cuts at: 5867 5867
Size: 7738
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5546 276
Size: 53 83 186 936 4012 2468
Fragments arranged by size:
4012 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SacII SapI SrfI SwaI