Restriction map for
pFN21AA0267
(Circular) (Six-base) MAPSORT of: pFN21AA0267.seq Check: 778 from: 1 to: 6712
pFN21AA0267 6712 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4522 278
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4055 1937
Size: 2118 4594
Acc65I G'GTAC_C
Cuts at: 4033 4033
Size: 6712
AclI AA'CG_TT
Cuts at: 1563 4839 5212 1563
Size: 3276 373 3063
Fragments arranged by size:
3276 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2884 3057 3662 3900 4775 6159 6427 1632
Size: 1252 173 605 238 875 1384 268 1917
Fragments arranged by size:
1917 1384 1252 875 605 268 238 173
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6515
AflIII A'CryG_T
Cuts at: 1203 5611 6588 1203
Size: 4408 977 1327
Fragments arranged by size:
4408 1327 977
AgeI A'CCGG_T
Cuts at: 5543 5543
Size: 6712
AhdI GACnn_n'nnGTC
Cuts at: 5441 5441
Size: 6712
AleI CACnn'nnGTG
Cuts at: 1166 3555 1166
Size: 2389 4323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2093 1184
Size: 909 5803
AlwNI CAG_nnn'CTG
Cuts at: 2693 2705 4455 6027 2693
Size: 12 1750 1572 3378
Fragments arranged by size:
3378 1750 1572 12
ApaI G_GGCC'C
Cuts at: 2293 2293
Size: 6712
ApaLI G'TGCA_C
Cuts at: 4769 5925 4769
Size: 1156 5556
ApoI r'AATT_y
Cuts at: 1493 3175 3343 3979 4021 4247 4399 6508 1493
Size: 1682 168 636 42 226 152 2109 1697
Fragments arranged by size:
2109 1697 1682 636 226 168 152 42
AseI AT'TA_AT
Cuts at: 160 1032 5266 160
Size: 872 4234 1606
Fragments arranged by size:
4234 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 4037 1870
Size: 73 118 1976 4545
Fragments arranged by size:
4545 1976 118 73
BamHI G'GATC_C
Cuts at: 4042 4409 4042
Size: 367 6345
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2675 3491 3714 4033
Size: 325 217 627 242 646 816 223 319
Cuts at: 4033 5488 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 816 646 627 325 319 242
223 217
BanII G_rGCy'C
Cuts at: 1391 2068 2293 4031 1391
Size: 677 225 1738 4072
Fragments arranged by size:
4072 1738 677 225
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5741
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2112 2146 4903 4937 1903
Size: 34 175 34 2757 34 3678
Fragments arranged by size:
3678 2757 175 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4604 5814 4604
Size: 1210 5502
BclI T'GATC_A
Cuts at: 1548 3628 1548
Size: 2080 4632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5323 136
Size: 107 122 71 631 605 3651 1525
Fragments arranged by size:
3651 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6707 6707
Size: 6712
BlpI GC'TnA_GC
Cuts at: 4129 4129
Size: 6712
Bme1580I G_kGCm'C
Cuts at: 1790 2032 2293 4773 5929 1790
Size: 242 261 2480 1156 2573
Fragments arranged by size:
2573 2480 1156 261 242
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6712
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2128 4443 5401
Size: 416 53 83 399 24 711 2315 958
Cuts at: 5401 442
Size: 1753
Fragments arranged by size:
2315 1753 958 711 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2853 2952 3037 5372 1239
Size: 1614 99 85 2335 2579
Fragments arranged by size:
2579 2335 1614 99 85
Bpu10I CC'TnA_GC
Cuts at: 2396 2396
Size: 6712
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4837 5702 6000 4837
Size: 865 298 5549
Fragments arranged by size:
5549 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5375 882
Size: 617 3876 2219
Fragments arranged by size:
3876 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3102 4503 493
Size: 711 1898 1401 2702
Fragments arranged by size:
2702 1898 1401 711
BsaBI GATnn'nnATC
Cuts at: 3771 6706 3771
Size: 2935 3777
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2109 4519 4901
Size: 53 83 186 936 513 63 2410 382
Cuts at: 4901 5536 275
Size: 635 1451
Fragments arranged by size:
2410 1451 936 635 513 382 186 83
63 53
BsaWI w'CCGG_w
Cuts at: 3609 5145 5543 5817 5964 6375 3609
Size: 1536 398 274 147 411 3946
Fragments arranged by size:
3946 1536 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2162 2192 2966 2996 3243
Size: 30 165 30 759 30 774 30 247
Cuts at: 3243 3273 1178
Size: 30 4617
Fragments arranged by size:
4617 774 759 247 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2183 2210 2797 1186
Size: 901 96 27 587 5101
Fragments arranged by size:
5101 901 587 96 27
BseYI C'CCAG_C
Cuts at: 5915 6411 5915
Size: 496 6216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 2429 1163
Size: 573 549 144 5446
Fragments arranged by size:
5446 573 549 144
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4162 4923 5951 1540
Size: 275 186 2161 761 1028 2301
Fragments arranged by size:
2301 2161 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4773 4858 5929 4773
Size: 85 1071 5556
Fragments arranged by size:
5556 1071 85
BsmI GAATG_Cn'
Cuts at: 2361 4223 4316 2361
Size: 1862 93 4757
Fragments arranged by size:
4757 1862 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2068 2293 4031 4773 4858 5929
Size: 399 242 36 225 1738 742 85 1071
Cuts at: 5929 1391
Size: 2174
Fragments arranged by size:
2174 1738 1071 742 399 242 225 85
36
BspEI T'CCGG_A
Cuts at: 3609 6375 3609
Size: 2766 3946
BspHI T'CATG_A
Cuts at: 4601 6331 4601
Size: 1730 4982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2232 2483 4067 844
Size: 1388 251 1584 3489
Fragments arranged by size:
3489 1584 1388 251
BsrBI CCG'CTC
Cuts at: 1394 1823 4599 1394
Size: 429 2776 3507
Fragments arranged by size:
3507 2776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2922 3390 3507 5207 5381 65
Size: 1183 1674 468 117 1700 174 1396
Fragments arranged by size:
1700 1674 1396 1183 468 174 117
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 2309 3835 5356 5543 1700
Size: 110 395 104 1526 1521 187 2869
Fragments arranged by size:
2869 1526 1521 395 187 110 104
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6712
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6712
BssSI C'ACGA_G
Cuts at: 2723 4772 5784 2723
Size: 2049 1012 3651
Fragments arranged by size:
3651 2049 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2921 3260 3596 794
Size: 2127 339 336 3910
Fragments arranged by size:
3910 2127 339 336
BstBI TT'CG_AA
Cuts at: 2524 5516 2524
Size: 2992 3720
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6712
BstXI CCAn_nnnn'nTGG
Cuts at: 2831 2831
Size: 6712
BstYI r'GATC_y
Cuts at: 1972 2883 2906 4042 4409 4794 4811 6263 6349
Size: 911 23 1136 367 385 17 1452 86
Cuts at: 6349 6361 6707 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1136 911 385 367 346 86
23 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2041 2160 3117 513
Size: 552 939 37 119 957 4108
Fragments arranged by size:
4108 957 939 552 119 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2879 3691 487
Size: 683 56 1653 812 3508
Fragments arranged by size:
3508 1653 812 683 56
BtsI GCAGTG_nn'
Cuts at: 2109 3495 4228 5022 5042 2109
Size: 1386 733 794 20 3779
Fragments arranged by size:
3779 1386 794 733 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6677
DraI TTT'AAA
Cuts at: 4016 4016
Size: 6712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5719 809
Size: 507 4403 1802
Fragments arranged by size:
4403 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3399 3837 4159
Size: 54 1425 50 275 18 1569 438 322
Cuts at: 4159 5048 6677 8
Size: 889 1629 43
Fragments arranged by size:
1629 1569 1425 889 438 322 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4159 1537
Size: 275 2347 4090
Fragments arranged by size:
4090 2347 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2279 3271 4642 1353
Size: 926 992 1371 3423
Fragments arranged by size:
3423 1371 992 926
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 2160 5299 5673 5819 387
Size: 1064 138 438 133 3139 374 146 1280
Fragments arranged by size:
3139 1280 1064 438 374 146 138 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2853 2884 2952 3037 3057 3662 3900
Size: 393 1221 31 68 85 20 605 238
Cuts at: 3900 4775 5372 6159 6427 1239
Size: 875 597 787 268 1524
Fragments arranged by size:
1524 1221 875 787 605 597 393 268
238 85 68 31 20
EcoRI G'AATT_C
Cuts at: 4021 4399 4021
Size: 378 6334
FspI TGC'GCA
Cuts at: 5218 5218
Size: 6712
HaeII r_GCGC'y
Cuts at: 1678 2220 3852 5859 1678
Size: 542 1632 2007 2531
Fragments arranged by size:
2531 2007 1632 542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2162 2194 2314 2346 3988
Size: 32 135 32 590 32 120 32 1642
Cuts at: 3988 4020 5353 5385 5427 5459 1373
Size: 32 1333 32 42 32 2626
Fragments arranged by size:
2626 1642 1333 590 135 120 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4056 4302 669
Size: 988 281 2118 246 3079
Fragments arranged by size:
3079 2118 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6712
HpaI GTT'AAC
Cuts at: 4302 4302
Size: 6712
KpnI G_GTAC'C
Cuts at: 4037 4037
Size: 6712
MfeI C'AATT_G
Cuts at: 4311 4311
Size: 6712
MluI A'CGCG_T
Cuts at: 6588 6588
Size: 6712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2444 2967 5826 6010 1305
Size: 1139 523 2859 184 2007
Fragments arranged by size:
2859 2007 1139 523 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3401 10
Size: 54 1425 343 1569 3321
Fragments arranged by size:
3321 1569 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2864 3359 3500 3555 4670 5029 5188
Size: 648 1698 495 141 55 1115 359 159
Cuts at: 5188 518
Size: 2042
Fragments arranged by size:
2042 1698 1115 648 495 359 159 141
55
MspA1I CmG'CkG
Cuts at: 1606 2831 3663 3673 4128 4805 5953 6198 1606
Size: 1225 832 10 455 677 1148 245 2120
Fragments arranged by size:
2120 1225 1148 832 677 455 245 10
NcoI C'CATG_G
Cuts at: 513 1065 2004 2160 513
Size: 552 939 156 5065
Fragments arranged by size:
5065 939 552 156
NdeI CA'TA_TG
Cuts at: 387 3808 387
Size: 3421 3291
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6712
NotI GC'GGCC_GC
Cuts at: 4159 4159
Size: 6712
NspI r_CATG'y
Cuts at: 1132 1229 4070 5615 1132
Size: 97 2841 1545 2229
Fragments arranged by size:
2841 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6712
PciI A'CATG_T
Cuts at: 5611 5611
Size: 6712
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2986 3922 1108
Size: 1878 936 3898
Fragments arranged by size:
3898 1878 936
PfoI T'CCnGG_A
Cuts at: 3014 3014
Size: 6712
PmeI GTTT'AAAC
Cuts at: 4016 4016
Size: 6712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4781 4813 6294 6326 4781
Size: 32 1481 32 5167
Fragments arranged by size:
5167 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2841 1613
Size: 1228 5484
PsiI TTA'TAA
Cuts at: 4282 4282
Size: 6712
PspOMI G'GGCC_C
Cuts at: 2289 2289
Size: 6712
PstI C_TGCA'G
Cuts at: 830 4064 830
Size: 3234 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6712
PvuII CAG'CTG
Cuts at: 2831 2831
Size: 6712
SalI G'TCGA_C
Cuts at: 1936 4054 1936
Size: 2118 4594
SapI GCTCTTCn'nnn_
Cuts at: 2279 3271 2279
Size: 992 5720
SbfI CC_TGCA'GG
Cuts at: 4064 4064
Size: 6712
ScaI AGT'ACT
Cuts at: 4960 6672 4960
Size: 1712 5000
SfcI C'TryA_G
Cuts at: 826 1045 4060 5195 5876 6067 826
Size: 219 3015 1135 681 191 1471
Fragments arranged by size:
3015 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6712
SmaI CCC'GGG
Cuts at: 1872 4039 1872
Size: 2167 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4816 5717 5979 820
Size: 197 926 2873 901 262 1553
Fragments arranged by size:
2873 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3102 4503 493
Size: 2609 1401 2702
Fragments arranged by size:
2702 2609 1401
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6712
SphI G_CATG'C
Cuts at: 1132 4070 1132
Size: 2938 3774
SspI AAT'ATT
Cuts at: 5 52 3289 4636 5
Size: 47 3237 1347 2081
Fragments arranged by size:
3237 2081 1347 47
StuI AGG'CCT
Cuts at: 1517 2997 1517
Size: 1480 5232
StyI C'CwwG_G
Cuts at: 513 1065 2004 2160 2890 3396 3854 3888 4151
Size: 552 939 156 730 506 458 34 263
Cuts at: 4151 513
Size: 3074
Fragments arranged by size:
3074 939 730 552 506 458 263 156
34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2909 3545 4739 4756 4909 5094 5539
Size: 1255 1329 636 1194 17 153 185 445
Cuts at: 5539 325
Size: 1498
Fragments arranged by size:
1498 1329 1255 1194 636 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4958 6670 96
Size: 274 80 33 51 1443 2981 1712 138
Fragments arranged by size:
2981 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6672 6704 6672
Size: 32 6680
Tth111I GACn'n_nGTC
Cuts at: 1316 4002 1316
Size: 2686 4026
XbaI T'CTAG_A
Cuts at: 4048 4048
Size: 6712
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6712
XmaI C'CCGG_G
Cuts at: 1870 4037 1870
Size: 2167 4545
XmnI GAAnn'nnTTC
Cuts at: 3874 4841 3874
Size: 967 5745
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4520 276
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI