Restriction map for
pFN21AA0260
(Circular) (Six-base) MAPSORT of: pFN21AA0260.seq Check: 7334 from: 1 to: 5770
pFN21AA0260 5770 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2026 3580 278
Size: 53 83 186 936 490 1554 2468
Fragments arranged by size:
2468 1554 936 490 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 1937
Size: 1176 4594
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 2472 3024 3833 5217 5485 1632
Size: 515 325 552 809 1384 268 1917
Fragments arranged by size:
1917 1384 809 552 515 325 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 4669 5646 1203
Size: 3466 977 1327
Fragments arranged by size:
3466 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 3513 5085 3513
Size: 1572 4198
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 3079 3305 3457 5566 1493
Size: 1586 226 152 2109 1697
Fragments arranged by size:
2109 1697 1586 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2888 4324 160
Size: 872 1856 1436 1606
Fragments arranged by size:
1856 1606 1436 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 3095 1870
Size: 73 91 1061 4545
Fragments arranged by size:
4545 1061 91 73
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3091 4546 618
Size: 325 217 627 1304 1455 1842
Fragments arranged by size:
1842 1455 1304 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2162 3089 1391
Size: 771 927 4072
Fragments arranged by size:
4072 927 771
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2477 928
Size: 971 578 4221
Fragments arranged by size:
4221 971 578
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2255 4381 136
Size: 107 122 71 631 605 583 2126 1525
Fragments arranged by size:
2126 1525 631 605 583 122 107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 3014 3187 3014
Size: 173 5597
Bme1580I G_kGCm'C
Cuts at: 1790 3831 4987 1790
Size: 2041 1156 2573
Fragments arranged by size:
2573 2041 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3501 4459 442
Size: 416 53 83 399 24 2084 958 1753
Fragments arranged by size:
2084 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2480 2480
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4430 1239
Size: 3191 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2450 3895 4760 5058 2450
Size: 1445 865 298 3162
Fragments arranged by size:
3162 1445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4433 882
Size: 617 2934 2219
Fragments arranged by size:
2934 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2617 2934 3561 493
Size: 711 1413 317 627 2702
Fragments arranged by size:
2702 1413 711 627 317
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2023 2099 3577 3959
Size: 53 83 186 936 490 76 1478 382
Cuts at: 3959 4594 275
Size: 635 1451
Fragments arranged by size:
1478 1451 936 635 490 382 186 83
76 53
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2511 2541 3054 3084 1178
Size: 30 165 30 1108 30 513 30 3864
Fragments arranged by size:
3864 1108 513 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2099 2206 1186
Size: 910 3 107 4750
Fragments arranged by size:
4750 910 107 3
BseYI C'CCAG_C
Cuts at: 2089 2644 2751 2969 4973 5469 2089
Size: 555 107 218 2004 496 2390
Fragments arranged by size:
2390 2004 555 496 218 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2815 1163
Size: 573 1079 4118
Fragments arranged by size:
4118 1079 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2106 3220 3981 5009 1540
Size: 275 186 105 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186 105
BsiHKAI G_wGCw'C
Cuts at: 2162 2196 3831 3916 4987 2162
Size: 34 1635 85 1071 2945
Fragments arranged by size:
2945 1635 1071 85 34
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2196 3089 3831 3916 4987 1391
Size: 399 372 34 893 742 85 1071 2174
Fragments arranged by size:
2174 1071 893 742 399 372 85 34
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 2682 3659 5389 2682
Size: 977 1730 3063
Fragments arranged by size:
3063 1730 977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3125 844
Size: 2281 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3657 1394
Size: 429 1834 3507
Fragments arranged by size:
3507 1834 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2514 2703 2964 4265 4439 65
Size: 1183 1266 189 261 1301 174 1396
Fragments arranged by size:
1396 1301 1266 1183 261 189 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 4414 4601 1700
Size: 110 331 2273 187 2869
Fragments arranged by size:
2869 2273 331 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2609 794
Size: 1815 3955
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 3100 3467 3852 3869 5321 5407 5419 5765
Size: 1128 367 385 17 1452 86 12 346
Cuts at: 5765 1972
Size: 1977
Fragments arranged by size:
1977 1452 1128 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5031
Fragments arranged by size:
5031 683 56
BtsI GCAGTG_nn'
Cuts at: 3072 3286 4080 4100 3072
Size: 214 794 20 4742
Fragments arranged by size:
4742 794 214 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 2955 3074 2955
Size: 119 5651
DraIII CAC_nnn'GTG
Cuts at: 2225 2225
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2136 2236
Size: 54 1425 50 275 18 273 33 100
Cuts at: 2236 2247 2700 3217 4106 5735 8
Size: 11 453 517 889 1629 43
Fragments arranged by size:
1629 1425 889 517 453 275 273 100
54 50 43 33 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2103 3217 1537
Size: 275 291 1114 4090
Fragments arranged by size:
4090 1114 291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3000 3700 1353
Size: 1647 700 3423
Fragments arranged by size:
3423 1647 700
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4357 4731 4877 387
Size: 1064 138 435 2333 374 146 1280
Fragments arranged by size:
2333 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2160 2160
Size: 5770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2472 3024 3833 4430 5217 5485
Size: 393 515 325 552 809 597 787 268
Cuts at: 5485 1239
Size: 1524
Fragments arranged by size:
1524 809 787 597 552 515 393 325
268
EcoRI G'AATT_C
Cuts at: 3079 3457 3079
Size: 378 5392
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 2282 4917 1678
Size: 604 2635 2531
Fragments arranged by size:
2635 2531 604
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4411 4443 4485 4517 1373
Size: 32 135 32 2839 32 42 32 2626
Fragments arranged by size:
2839 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2209 4884 5068 1305
Size: 904 2675 184 2007
Fragments arranged by size:
2675 2007 904 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2238 2249 2702 10
Size: 54 1425 343 406 11 453 3078
Fragments arranged by size:
3078 1425 453 406 343 54 11
MslI CAynn'nnrTG
Cuts at: 518 1166 3728 4087 4246 518
Size: 648 2562 359 159 2042
Fragments arranged by size:
2562 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3013 3186 3863 5011 5256 1606
Size: 1407 173 677 1148 245 2120
Fragments arranged by size:
2120 1407 1148 677 245 173
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 5770
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 2033 3128 4673 1132
Size: 97 804 1095 1545 2229
Fragments arranged by size:
2229 1545 1095 804 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5770
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 1108
Size: 1137 4633
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PmlI CAC'GTG
Cuts at: 2934 2934
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 2778 3122 830
Size: 1948 344 3478
Fragments arranged by size:
3478 1948 344
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
PvuII CAG'CTG
Cuts at: 3013 3013
Size: 5770
SacI G_AGCT'C
Cuts at: 2162 2162
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SapI GCTCTTCn'nnn_
Cuts at: 3000 3000
Size: 5770
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 2826 4018 5730 2826
Size: 1192 1712 2866
Fragments arranged by size:
2866 1712 1192
SfcI C'TryA_G
Cuts at: 826 1045 2774 3118 4253 4934 5125 826
Size: 219 1729 344 1135 681 191 1471
Fragments arranged by size:
1729 1471 1135 681 344 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2465 3874 4775 5037 820
Size: 197 926 522 1409 901 262 1553
Fragments arranged by size:
1553 1409 926 901 522 262 197
SnaBI TAC'GTA
Cuts at: 493 2617 3561 493
Size: 2124 944 2702
Fragments arranged by size:
2702 2124 944
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 2033 3128 1132
Size: 901 1095 3774
Fragments arranged by size:
3774 1095 901
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2004 3209 513
Size: 552 939 1205 3074
Fragments arranged by size:
3074 1205 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2257 3797 3814 3967 4152 4597 325
Size: 1255 677 1540 17 153 185 445 1498
Fragments arranged by size:
1540 1498 1255 677 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2824 4016 5728
Size: 274 80 33 51 1443 847 1192 1712
Cuts at: 5728 96
Size: 138
Fragments arranged by size:
1712 1443 1192 847 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5770
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2542 2542
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2024 3578 276
Size: 53 83 186 936 490 1554 2468
Fragments arranged by size:
2468 1554 936 490 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI