Restriction map for
pFN21AA0259
(Circular) (Six-base) MAPSORT of: pFN21AA0259.seq Check: 5913 from: 1 to: 9271
pFN21AA0259 9271 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3524 4558 7081 278
Size: 53 83 186 936 1988 1034 2523 2468
Fragments arranged by size:
2523 2468 1988 1034 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2409 5041 5112 6460 6614 1937
Size: 472 2632 71 1348 154 4594
Fragments arranged by size:
4594 2632 1348 472 154 71
Acc65I G'GTAC_C
Cuts at: 6592 6592
Size: 9271
AclI AA'CG_TT
Cuts at: 1563 7398 7771 1563
Size: 5835 373 3063
Fragments arranged by size:
5835 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2037 2105 2510 3090 3339 3546 3669 4424
Size: 405 68 405 580 249 207 123 755
Cuts at: 4424 5507 5649 5673 6233 7334 8718 8986 1632
Size: 1083 142 24 560 1101 1384 268 1917
Fragments arranged by size:
1917 1384 1101 1083 755 580 560 405
405 268 249 207 142 123 68 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9074
AflIII A'CryG_T
Cuts at: 1203 5620 6208 6384 6568 8170 9147 1203
Size: 4417 588 176 184 1602 977 1327
Fragments arranged by size:
4417 1602 1327 977 588 184 176
AgeI A'CCGG_T
Cuts at: 8102 8102
Size: 9271
AhdI GACnn_n'nnGTC
Cuts at: 8000 8000
Size: 9271
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4328 1184
Size: 3144 6127
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3147 3179 3315 3347 4011 4043 3147
Size: 32 136 32 664 32 8375
Fragments arranged by size:
8375 664 136 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4289 5396 6371 7014 8586 4289
Size: 1107 975 643 1572 4974
Fragments arranged by size:
4974 1572 1107 975 643
ApaLI G'TGCA_C
Cuts at: 7328 8484 7328
Size: 1156 8115
ApoI r'AATT_y
Cuts at: 1493 2099 3357 4138 4222 4279 4478 6580 6806
Size: 606 1258 781 84 57 199 2102 226
Cuts at: 6806 6958 9067 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 2102 1697 1258 781 606 226 199
152 84 57
AseI AT'TA_AT
Cuts at: 160 1032 7825 160
Size: 872 6793 1606
Fragments arranged by size:
6793 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9271
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 6222 6596 1870
Size: 73 141 4138 374 4545
Fragments arranged by size:
4545 4138 374 141 73
BamHI G'GATC_C
Cuts at: 6601 6968 6601
Size: 367 8904
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6592 8047 618
Size: 325 217 627 4805 1455 1842
Fragments arranged by size:
4805 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3564 4452 6590 1391
Size: 2173 888 2138 4072
Fragments arranged by size:
4072 2173 2138 888
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2043 3145 3600 5052 6051 6115 928
Size: 971 144 1102 455 1452 999 64 4084
Fragments arranged by size:
4084 1452 1102 999 971 455 144 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7462 7496 1903
Size: 34 5525 34 3678
Fragments arranged by size:
5525 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4459 7163 8373 4459
Size: 2704 1210 5357
Fragments arranged by size:
5357 2704 1210
BclI T'GATC_A
Cuts at: 1548 2209 1548
Size: 661 8610
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5969 7882 136
Size: 107 122 71 631 605 4297 1913 1525
Fragments arranged by size:
4297 1913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3469 9266 3469
Size: 5797 3474
BlpI GC'TnA_GC
Cuts at: 3558 6688 3558
Size: 3130 6141
Bme1580I G_kGCm'C
Cuts at: 1790 4939 7332 8488 1790
Size: 3149 2393 1156 2573
Fragments arranged by size:
3149 2573 2393 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9271
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4681 5703 7002
Size: 416 53 83 399 24 3264 1022 1299
Cuts at: 7002 7960 442
Size: 958 1753
Fragments arranged by size:
3264 1753 1299 1022 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9271
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5397 6546 5397
Size: 1149 8122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3035 3996 4533 7931 1239
Size: 1796 961 537 3398 2579
Fragments arranged by size:
3398 2579 1796 961 537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2232 5685 5820 6415 7396 8261 8559 2232
Size: 3453 135 595 981 865 298 2944
Fragments arranged by size:
3453 2944 981 865 595 298 135
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2409 4393 7934 882
Size: 617 910 1984 3541 2219
Fragments arranged by size:
3541 2219 1984 910 617
BsaAI yAC'GTr
Cuts at: 493 1204 5621 7062 493
Size: 711 4417 1441 2702
Fragments arranged by size:
4417 2702 1441 711
BsaBI GATnn'nnATC
Cuts at: 3468 9265 3468
Size: 5797 3474
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3521 4555 7078 7460
Size: 53 83 186 936 1988 1034 2523 382
Cuts at: 7460 8095 275
Size: 635 1451
Fragments arranged by size:
2523 1988 1451 1034 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 7704 8102 8376 8523 8934 7704
Size: 398 274 147 411 8041
Fragments arranged by size:
8041 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3147 3177 3819 3849 4011
Size: 30 165 30 1744 30 642 30 162
Cuts at: 4011 4041 4439 4469 5225 5255 5617 5647 6252
Size: 30 398 30 756 30 362 30 605
Cuts at: 6252 6282 1178
Size: 30 4167
Fragments arranged by size:
4167 1744 756 642 605 398 362 165
162 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4911 5474 5792 6273 6429 1186
Size: 3725 563 318 481 156 4028
Fragments arranged by size:
4028 3725 563 481 318 156
BseYI C'CCAG_C
Cuts at: 3740 3799 4420 4559 5975 8474 8970 3740
Size: 59 621 139 1416 2499 496 4041
Fragments arranged by size:
4041 2499 1416 621 496 139 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 6056 1163
Size: 573 4320 4378
Fragments arranged by size:
4378 4320 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6721 7482 8510 1540
Size: 275 186 4720 761 1028 2301
Fragments arranged by size:
4720 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4920 7332 7417 8488 4920
Size: 2412 85 1071 5703
Fragments arranged by size:
5703 2412 1071 85
BsmI GAATG_Cn'
Cuts at: 6782 6875 6782
Size: 93 9178
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3564 4452 4920 4939 6590 7332 7417
Size: 399 1774 888 468 19 1651 742 85
Cuts at: 7417 8488 1391
Size: 1071 2174
Fragments arranged by size:
2174 1774 1651 1071 888 742 468 399
85 19
BspEI T'CCGG_A
Cuts at: 8934 8934
Size: 9271
BspHI T'CATG_A
Cuts at: 5785 7160 8890 5785
Size: 1375 1730 6166
Fragments arranged by size:
6166 1730 1375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4153 6626 844
Size: 3309 2473 3489
Fragments arranged by size:
3489 3309 2473
BsrBI CCG'CTC
Cuts at: 1394 1823 7158 1394
Size: 429 5335 3507
Fragments arranged by size:
5335 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6132 6408 7766 7940 65
Size: 1183 4884 276 1358 174 1396
Fragments arranged by size:
4884 1396 1358 1183 276 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5025 7915 8102 1700
Size: 110 3215 2890 187 2869
Fragments arranged by size:
3215 2890 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2342 2386 2717 2779 5915 6097 96
Size: 2246 44 331 62 3136 182 3270
Fragments arranged by size:
3270 3136 2246 331 182 62 44
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9271
BssSI C'ACGA_G
Cuts at: 7331 8343 7331
Size: 1012 8259
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5474 6371 794
Size: 4680 897 3694
Fragments arranged by size:
4680 3694 897
BstBI TT'CG_AA
Cuts at: 5944 8075 5944
Size: 2131 7140
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9271
BstXI CCAn_nnnn'nTGG
Cuts at: 3974 3974
Size: 9271
BstYI r'GATC_y
Cuts at: 1972 3469 5083 6601 6968 7353 7370 8822 8908
Size: 1497 1614 1518 367 385 17 1452 86
Cuts at: 8908 8920 9266 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1614 1518 1497 1452 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2410 2410
Size: 9271
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 8719
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2293 3700 5978 487
Size: 683 56 1067 1407 2278 3780
Fragments arranged by size:
3780 2278 1407 1067 683 56
BtsI GCAGTG_nn'
Cuts at: 5019 5350 5495 5903 6787 7581 7601 5019
Size: 331 145 408 884 794 20 6689
Fragments arranged by size:
6689 884 794 408 331 145 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3490 3525 529
Size: 35 2926 35 6275
Fragments arranged by size:
6275 2926 35 35
DraI TTT'AAA
Cuts at: 2046 2077 3399 6298 6575 2046
Size: 31 1322 2899 277 4742
Fragments arranged by size:
4742 2899 1322 277 31
DraIII CAC_nnn'GTG
Cuts at: 2788 2799 6034 2788
Size: 11 3235 6025
Fragments arranged by size:
6025 3235 11
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8278 809
Size: 507 6962 1802
Fragments arranged by size:
6962 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4853 5023 5121
Size: 54 1425 50 275 18 3023 170 98
Cuts at: 5121 6718 7607 9236 8
Size: 1597 889 1629 43
Fragments arranged by size:
3023 1629 1597 1425 889 275 170 98
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6718 1537
Size: 275 4906 4090
Fragments arranged by size:
4906 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 3908 4505 7201 1353
Size: 780 1775 597 2696 3423
Fragments arranged by size:
3423 2696 1775 780 597
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2415 7858 8232 8378 387
Size: 1064 138 826 5443 374 146 1280
Fragments arranged by size:
5443 1280 1064 826 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2105 2510 3035 3090 3339 3546
Size: 393 405 68 405 525 55 249 207
Cuts at: 3546 3669 3996 4424 4533 5507 5649 5673 6233
Size: 123 327 428 109 974 142 24 560
Cuts at: 6233 7334 7931 8718 8986 1239
Size: 1101 597 787 268 1524
Fragments arranged by size:
1524 1101 974 787 597 560 525 428
405 405 393 327 268 249 207 142
123 109 68 55 24
EcoNI CCTnn'n_nnAGG
Cuts at: 4487 4487
Size: 9271
EcoO109I rG'GnC_Cy
Cuts at: 3534 3534
Size: 9271
EcoRI G'AATT_C
Cuts at: 2099 6580 6958 2099
Size: 4481 378 4412
Fragments arranged by size:
4481 4412 378
EcoRV GAT'ATC
Cuts at: 5005 5005
Size: 9271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2135 3410 3448 4528 5917 2135
Size: 1275 38 1080 1389 5489
Fragments arranged by size:
5489 1389 1275 1080 38
FspI TGC'GCA
Cuts at: 7777 7777
Size: 9271
HaeII r_GCGC'y
Cuts at: 1678 8418 1678
Size: 6740 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3461 3493 3875 3907 4457
Size: 32 135 32 1889 32 382 32 550
Cuts at: 4457 4489 4673 4705 4865 4897 5225 5257 5617
Size: 32 184 32 160 32 328 32 360
Cuts at: 5617 5649 7912 7944 7986 8018 1373
Size: 32 2263 32 42 32 2626
Fragments arranged by size:
2626 2263 1889 550 382 360 328 184
160 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5042 5404 5530 6615 6861 669
Size: 988 281 3104 362 126 1085 246 3079
Fragments arranged by size:
3104 3079 1085 988 362 281 246 126
HindIII A'AGCT_T
Cuts at: 748 2222 4371 748
Size: 1474 2149 5648
Fragments arranged by size:
5648 2149 1474
HpaI GTT'AAC
Cuts at: 6861 6861
Size: 9271
KpnI G_GTAC'C
Cuts at: 6596 6596
Size: 9271
MfeI C'AATT_G
Cuts at: 2699 2919 6870 2699
Size: 220 3951 5100
Fragments arranged by size:
5100 3951 220
MluI A'CGCG_T
Cuts at: 9147 9147
Size: 9271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2982 3071 4149 8385 8569 1305
Size: 1677 89 1078 4236 184 2007
Fragments arranged by size:
4236 2007 1677 1078 184 89
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5123 10
Size: 54 1425 343 3291 4158
Fragments arranged by size:
4158 3291 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2382 5621 5927 7229 7588 7747 518
Size: 648 1216 3239 306 1302 359 159 2042
Fragments arranged by size:
3239 2042 1302 1216 648 359 306 159
MspA1I CmG'CkG
Cuts at: 1606 5054 5975 6687 7364 8512 8757 1606
Size: 3448 921 712 677 1148 245 2120
Fragments arranged by size:
3448 2120 1148 921 712 677 245
NaeI GCC'GGC
Cuts at: 5027 5027
Size: 9271
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8719
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9271
NgoMIV G'CCGG_C
Cuts at: 5025 5025
Size: 9271
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9271
NotI GC'GGCC_GC
Cuts at: 6718 6718
Size: 9271
NspI r_CATG'y
Cuts at: 1132 1229 4072 4400 6212 6629 8174 1132
Size: 97 2843 328 1812 417 1545 2229
Fragments arranged by size:
2843 2229 1812 1545 417 328 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9271
PciI A'CATG_T
Cuts at: 6208 8170 6208
Size: 1962 7309
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2772 2799 3352 5153 1108
Size: 1664 27 553 1801 5226
Fragments arranged by size:
5226 1801 1664 553 27
PfoI T'CCnGG_A
Cuts at: 4549 5608 4549
Size: 1059 8212
PmeI GTTT'AAAC
Cuts at: 6575 6575
Size: 9271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3147 3179 4011 4043 7340 7372 8853 8885 3147
Size: 32 832 32 3297 32 1481 32 3533
Fragments arranged by size:
3533 3297 1481 832 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 5853 1613
Size: 4240 5031
PsiI TTA'TAA
Cuts at: 2310 4983 5768 6841 2310
Size: 2673 785 1073 4740
Fragments arranged by size:
4740 2673 1073 785
PstI C_TGCA'G
Cuts at: 830 2234 4294 6017 6623 830
Size: 1404 2060 1723 606 3478
Fragments arranged by size:
3478 2060 1723 1404 606
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9271
PvuII CAG'CTG
Cuts at: 5054 5975 5054
Size: 921 8350
SalI G'TCGA_C
Cuts at: 1936 5040 6613 1936
Size: 3104 1573 4594
Fragments arranged by size:
4594 3104 1573
SbfI CC_TGCA'GG
Cuts at: 6017 6623 6017
Size: 606 8665
ScaI AGT'ACT
Cuts at: 4081 7519 9231 4081
Size: 3438 1712 4121
Fragments arranged by size:
4121 3438 1712
SexAI A'CCwGG_T
Cuts at: 6274 6274
Size: 9271
SfcI C'TryA_G
Cuts at: 826 1045 2230 4155 4290 5448 6013 6534 6619
Size: 219 1185 1925 135 1158 565 521 85
Cuts at: 6619 7754 8435 8626 826
Size: 1135 681 191 1471
Fragments arranged by size:
1925 1471 1185 1158 1135 681 565 521
219 191 135 85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9271
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9271
SmaI CCC'GGG
Cuts at: 1872 6598 1872
Size: 4726 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2084 2247 5700 5835 6222 6394
Size: 197 926 141 163 3453 135 387 172
Cuts at: 6394 7375 8276 8538 820
Size: 981 901 262 1553
Fragments arranged by size:
3453 1553 981 926 901 387 262 197
172 163 141 135
SnaBI TAC'GTA
Cuts at: 493 7062 493
Size: 6569 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9271
SphI G_CATG'C
Cuts at: 1132 6629 1132
Size: 5497 3774
SspI AAT'ATT
Cuts at: 5 52 2471 2953 7195 5
Size: 47 2419 482 4242 2081
Fragments arranged by size:
4242 2419 2081 482 47
StuI AGG'CCT
Cuts at: 1517 3259 1517
Size: 1742 7529
StyI C'CwwG_G
Cuts at: 513 1065 4015 4488 4539 5266 6710 513
Size: 552 2950 473 51 727 1444 3074
Fragments arranged by size:
3074 2950 1444 727 552 473 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5658 5974 6180 7298 7315 7468 7653
Size: 1255 4078 316 206 1118 17 153 185
Cuts at: 7653 8098 325
Size: 445 1498
Fragments arranged by size:
4078 1498 1255 1118 445 316 206 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2342 2386 2717
Size: 274 80 33 51 1443 365 44 331
Cuts at: 2717 2779 4079 4900 5805 5915 6097 6387 6565
Size: 62 1300 821 905 110 182 290 178
Cuts at: 6565 7517 9229 96
Size: 952 1712 138
Fragments arranged by size:
1712 1443 1300 952 905 821 365 331
290 274 182 178 138 110 80 62
51 44 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3898 3930 4895 4927 6018 6050 6252 6284 9231
Size: 32 965 32 1091 32 202 32 2947
Cuts at: 9231 9263 3898
Size: 32 3906
Fragments arranged by size:
3906 2947 1091 965 202 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9271
XbaI T'CTAG_A
Cuts at: 3034 6607 3034
Size: 3573 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4996 5737 4996
Size: 741 8530
XhoI C'TCGA_G
Cuts at: 1943 2084 6222 1943
Size: 141 4138 4992
Fragments arranged by size:
4992 4138 141
XmaI C'CCGG_G
Cuts at: 1870 6596 1870
Size: 4726 4545
XmnI GAAnn'nnTTC
Cuts at: 2118 2373 6064 7400 2118
Size: 255 3691 1336 3989
Fragments arranged by size:
3989 3691 1336 255
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3522 4556 7079 276
Size: 53 83 186 936 1988 1034 2523 2468
Fragments arranged by size:
2523 2468 1988 1034 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI Bsu36I EcoICRI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI