Restriction map for
pFN21AA0256
(Circular) (Six-base) MAPSORT of: pFN21AA0256.seq Check: 3620 from: 1 to: 7873
pFN21AA0256 7873 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2912 2912
Size: 7873
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5683 278
Size: 53 83 186 936 4147 2468
Fragments arranged by size:
4147 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2321 5216 1937
Size: 384 2895 4594
Fragments arranged by size:
4594 2895 384
Acc65I G'GTAC_C
Cuts at: 4387 5194 4387
Size: 807 7066
AclI AA'CG_TT
Cuts at: 1563 6000 6373 1563
Size: 4437 373 3063
Fragments arranged by size:
4437 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4491 4506 4946 5936 7320 7588 1632
Size: 2859 15 440 990 1384 268 1917
Fragments arranged by size:
2859 1917 1384 990 440 268 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7676
AflIII A'CryG_T
Cuts at: 1203 3351 6772 7749 1203
Size: 2148 3421 977 1327
Fragments arranged by size:
3421 2148 1327 977
AgeI A'CCGG_T
Cuts at: 6704 6704
Size: 7873
AhdI GACnn_n'nnGTC
Cuts at: 6602 6602
Size: 7873
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7873
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7873
AlwNI CAG_nnn'CTG
Cuts at: 2618 4751 5118 5616 7188 2618
Size: 2133 367 498 1572 3303
Fragments arranged by size:
3303 2133 1572 498 367
ApaLI G'TGCA_C
Cuts at: 5930 7086 5930
Size: 1156 6717
ApoI r'AATT_y
Cuts at: 1493 4170 4786 5182 5408 5560 7669 1493
Size: 2677 616 396 226 152 2109 1697
Fragments arranged by size:
2677 2109 1697 616 396 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6427 160
Size: 872 5395 1606
Fragments arranged by size:
5395 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7873
AvaI C'yCGr_G
Cuts at: 1870 1943 5198 1870
Size: 73 3255 4545
Fragments arranged by size:
4545 3255 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4803 4836 4803
Size: 33 7840
BamHI G'GATC_C
Cuts at: 3662 5203 5570 3662
Size: 1541 367 5965
Fragments arranged by size:
5965 1541 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4387 5032 5194 6649 618
Size: 325 217 627 2600 645 162 1455 1842
Fragments arranged by size:
2600 1842 1455 645 627 325 217 162
BanII G_rGCy'C
Cuts at: 1391 2017 5192 1391
Size: 626 3175 4072
Fragments arranged by size:
4072 3175 626
BbeI G_GCGC'C
Cuts at: 5036 5036
Size: 7873
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2467 5001 928
Size: 971 568 2534 3800
Fragments arranged by size:
3800 2534 971 568
BbvCI CC'TCA_GC
Cuts at: 2046 4359 4660 2046
Size: 2313 301 5259
Fragments arranged by size:
5259 2313 301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6064 6098 1903
Size: 34 4127 34 3678
Fragments arranged by size:
4127 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4570 5765 6975 4570
Size: 1195 1210 5468
Fragments arranged by size:
5468 1210 1195
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7873
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6484 136
Size: 107 122 71 631 605 4812 1525
Fragments arranged by size:
4812 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4777 7868 4777
Size: 3091 4782
BlpI GC'TnA_GC
Cuts at: 2298 5290 2298
Size: 2992 4881
Bme1580I G_kGCm'C
Cuts at: 1790 2356 5934 7090 1790
Size: 566 3578 1156 2573
Fragments arranged by size:
3578 2573 1156 566
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7873
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3747 4584 5604
Size: 416 53 83 399 24 2330 837 1020
Cuts at: 5604 6562 442
Size: 958 1753
Fragments arranged by size:
2330 1753 1020 958 837 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7873
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4771 4771
Size: 7873
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3756 5066 6533 1239
Size: 2517 1310 1467 2579
Fragments arranged by size:
2579 2517 1467 1310
Bpu10I CC'TnA_GC
Cuts at: 2046 3081 4359 4660 2046
Size: 1035 1278 301 5259
Fragments arranged by size:
5259 1278 1035 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3231 5998 6863 7161 3231
Size: 2767 865 298 3943
Fragments arranged by size:
3943 2767 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2699 4931 4936 6536 882
Size: 617 1200 2232 5 1600 2219
Fragments arranged by size:
2232 2219 1600 1200 617 5
BsaAI yAC'GTr
Cuts at: 493 1204 5664 493
Size: 711 4460 2702
Fragments arranged by size:
4460 2702 711
BsaBI GATnn'nnATC
Cuts at: 7867 7867
Size: 7873
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5033 5680 6062 6697
Size: 53 83 186 936 3500 647 382 635
Cuts at: 6697 275
Size: 1451
Fragments arranged by size:
3500 1451 936 647 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6306 6704 6978 7125 7536 6306
Size: 398 274 147 411 6643
Fragments arranged by size:
6643 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7648
Fragments arranged by size:
7648 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4319 4871 4874 4877 1186
Size: 3133 552 3 3 4182
Fragments arranged by size:
4182 3133 552 3 3
BseYI C'CCAG_C
Cuts at: 2156 4673 7076 7572 2156
Size: 2517 2403 496 2457
Fragments arranged by size:
2517 2457 2403 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 2940 3353 4922 1163
Size: 573 471 733 413 1569 4114
Fragments arranged by size:
4114 1569 733 573 471 413
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5323 6084 7112 1540
Size: 275 186 3322 761 1028 2301
Fragments arranged by size:
3322 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5142 5934 6019 7090 5142
Size: 792 85 1071 5925
Fragments arranged by size:
5925 1071 792 85
BsmI GAATG_Cn'
Cuts at: 2447 5384 5477 2447
Size: 2937 93 4843
Fragments arranged by size:
4843 2937 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4009 4009
Size: 7873
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2356 5142 5192 5934 6019 7090
Size: 399 227 339 2786 50 742 85 1071
Cuts at: 7090 1391
Size: 2174
Fragments arranged by size:
2786 2174 1071 742 399 339 227 85
50
BspEI T'CCGG_A
Cuts at: 7536 7536
Size: 7873
BspHI T'CATG_A
Cuts at: 4879 5762 7492 4879
Size: 883 1730 5260
Fragments arranged by size:
5260 1730 883
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2603 2912 5228 844
Size: 1759 309 2316 3489
Fragments arranged by size:
3489 2316 1759 309
BsrBI CCG'CTC
Cuts at: 1394 1823 5760 1394
Size: 429 3937 3507
Fragments arranged by size:
3937 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3390 3525 4347 6368 6542 65
Size: 1183 2142 135 822 2021 174 1396
Fragments arranged by size:
2142 2021 1396 1183 822 174 135
BsrFI r'CCGG_y
Cuts at: 1700 1810 6517 6704 1700
Size: 110 4707 187 2869
Fragments arranged by size:
4707 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7873
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7873
BssSI C'ACGA_G
Cuts at: 5933 6945 5933
Size: 1012 6861
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2351 3236 4430 5075 794
Size: 1557 885 1194 645 3592
Fragments arranged by size:
3592 1557 1194 885 645
BstBI TT'CG_AA
Cuts at: 6677 6677
Size: 7873
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7873
BstYI r'GATC_y
Cuts at: 1972 3662 3991 4777 5203 5570 5955 5972 7424
Size: 1690 329 786 426 367 385 17 1452
Cuts at: 7424 7510 7522 7868 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1690 1452 786 426 385 367 346
329 86 17 12
BstZ17I GTA'TAC
Cuts at: 2322 2322
Size: 7873
Bsu36I CC'TnA_GG
Cuts at: 5037 5037
Size: 7873
BtgI C'CryG_G
Cuts at: 513 1065 2004 2019 4152 513
Size: 552 939 15 2133 4234
Fragments arranged by size:
4234 2133 939 552 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7134
Fragments arranged by size:
7134 683 56
BtsI GCAGTG_nn'
Cuts at: 2709 4453 5389 6183 6203 2709
Size: 1744 936 794 20 4379
Fragments arranged by size:
4379 1744 936 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7873
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7838
DraI TTT'AAA
Cuts at: 3609 5177 3609
Size: 1568 6305
DraIII CAC_nnn'GTG
Cuts at: 2351 2351
Size: 7873
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3808 6880 809
Size: 507 2492 3072 1802
Fragments arranged by size:
3072 2492 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5320 6209 7838
Size: 54 1425 50 275 18 3490 889 1629
Cuts at: 7838 8
Size: 43
Fragments arranged by size:
3490 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5320 1537
Size: 275 3508 4090
Fragments arranged by size:
4090 3508 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2069 2743 3948 5013 5803 1353
Size: 716 674 1205 1065 790 3423
Fragments arranged by size:
3423 1205 1065 790 716 674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6460 6834 6980 387
Size: 1064 138 4871 374 146 1280
Fragments arranged by size:
4871 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3756 4491 4506 4946 5066 5936 6533
Size: 393 2124 735 15 440 120 870 597
Cuts at: 6533 7320 7588 1239
Size: 787 268 1524
Fragments arranged by size:
2124 1524 870 787 735 597 440 393
268 120 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2859 2859
Size: 7873
EcoO109I rG'GnC_Cy
Cuts at: 2615 2615
Size: 7873
EcoRI G'AATT_C
Cuts at: 4786 5182 5560 4786
Size: 396 378 7099
Fragments arranged by size:
7099 396 378
EcoRV GAT'ATC
Cuts at: 2683 2683
Size: 7873
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3652 3652
Size: 7873
FspI TGC'GCA
Cuts at: 6379 6379
Size: 7873
HaeII r_GCGC'y
Cuts at: 1678 3637 5036 7020 1678
Size: 1959 1399 1984 2531
Fragments arranged by size:
2531 1984 1959 1399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2508 2540 2612 2644 2660
Size: 32 135 32 936 32 72 32 16
Cuts at: 2660 2692 2881 2913 6514 6546 6588 6620 1373
Size: 32 189 32 3601 32 42 32 2626
Fragments arranged by size:
3601 2626 936 189 135 72 42 32
32 32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 5217 5463 669
Size: 988 281 3279 246 3079
Fragments arranged by size:
3279 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3219 3291 748
Size: 2471 72 5330
Fragments arranged by size:
5330 2471 72
HpaI GTT'AAC
Cuts at: 5463 5463
Size: 7873
KasI G'GCGC_C
Cuts at: 5032 5032
Size: 7873
KpnI G_GTAC'C
Cuts at: 4391 5198 4391
Size: 807 7066
MfeI C'AATT_G
Cuts at: 4617 5472 4617
Size: 855 7018
MluI A'CGCG_T
Cuts at: 7749 7749
Size: 7873
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 6987 7171 1305
Size: 1116 4566 184 2007
Fragments arranged by size:
4566 2007 1116 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6051
Fragments arranged by size:
6051 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2914 5831 6190 6349 518
Size: 648 1748 2917 359 159 2042
Fragments arranged by size:
2917 2042 1748 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2335 2572 3308 5289 5966 7114 7359
Size: 439 290 237 736 1981 677 1148 245
Cuts at: 7359 1606
Size: 2120
Fragments arranged by size:
2120 1981 1148 736 677 439 290 245
237
NarI GG'CG_CC
Cuts at: 5033 5033
Size: 7873
NcoI C'CATG_G
Cuts at: 513 1065 2004 4152 513
Size: 552 939 2148 4234
Fragments arranged by size:
4234 2148 939 552
NdeI CA'TA_TG
Cuts at: 387 4053 387
Size: 3666 4207
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7873
NotI GC'GGCC_GC
Cuts at: 5320 5320
Size: 7873
NspI r_CATG'y
Cuts at: 1132 1229 3355 3751 4831 5231 6776 1132
Size: 97 2126 396 1080 400 1545 2229
Fragments arranged by size:
2229 2126 1545 1080 400 396 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7873
PciI A'CATG_T
Cuts at: 3351 6772 3351
Size: 3421 4452
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2362 1108
Size: 1254 6619
PfoI T'CCnGG_A
Cuts at: 3969 3969
Size: 7873
PmeI GTTT'AAAC
Cuts at: 5177 5177
Size: 7873
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5942 5974 7455 7487 5942
Size: 32 1481 32 6328
Fragments arranged by size:
6328 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2615 2615
Size: 7873
PshAI GACnn'nnGTC
Cuts at: 1613 3418 1613
Size: 1805 6068
PsiI TTA'TAA
Cuts at: 4082 5443 4082
Size: 1361 6512
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 7841
PstI C_TGCA'G
Cuts at: 830 2831 2860 3442 4426 4448 5123 5225 830
Size: 2001 29 582 984 22 675 102 3478
Fragments arranged by size:
3478 2001 984 675 582 102 29 22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7873
PvuII CAG'CTG
Cuts at: 2045 2335 3308 2045
Size: 290 973 6610
Fragments arranged by size:
6610 973 290
SalI G'TCGA_C
Cuts at: 1936 5215 1936
Size: 3279 4594
SapI GCTCTTCn'nnn_
Cuts at: 2743 2743
Size: 7873
SbfI CC_TGCA'GG
Cuts at: 5225 5225
Size: 7873
ScaI AGT'ACT
Cuts at: 4654 6121 7833 4654
Size: 1467 1712 4694
Fragments arranged by size:
4694 1712 1467
SfcI C'TryA_G
Cuts at: 826 1045 2313 2827 2856 3438 4422 4444 4644
Size: 219 1268 514 29 582 984 22 200
Cuts at: 4644 4893 5119 5221 6356 7037 7228 826
Size: 249 226 102 1135 681 191 1471
Fragments arranged by size:
1471 1268 1135 984 681 582 514 249
226 219 200 191 102 29 22
SfoI GGC'GCC
Cuts at: 5034 5034
Size: 7873
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7873
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7873
SmaI CCC'GGG
Cuts at: 1872 5200 1872
Size: 3328 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3246 5977 6878 7140 820
Size: 197 926 1303 2731 901 262 1553
Fragments arranged by size:
2731 1553 1303 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5664 493
Size: 5171 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7873
SphI G_CATG'C
Cuts at: 1132 3751 4831 5231 1132
Size: 2619 1080 400 3774
Fragments arranged by size:
3774 2619 1080 400
SspI AAT'ATT
Cuts at: 5 52 3094 5797 5
Size: 47 3042 2703 2081
Fragments arranged by size:
3042 2703 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7873
StyI C'CwwG_G
Cuts at: 513 1065 2004 2733 2972 4152 4189 5312 513
Size: 552 939 729 239 1180 37 1123 3074
Fragments arranged by size:
3074 1180 1123 939 729 552 239 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2025 5900 5917 6070 6255 6700 325
Size: 1255 445 3875 17 153 185 445 1498
Fragments arranged by size:
3875 1498 1255 445 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4652 4742 6119
Size: 274 80 33 51 1443 2675 90 1377
Cuts at: 6119 7831 96
Size: 1712 138
Fragments arranged by size:
2675 1712 1443 1377 274 138 90 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2437 2469 3172 3204 4200 4232 5047 5079 7833
Size: 32 703 32 996 32 815 32 2754
Cuts at: 7833 7865 2437
Size: 32 2445
Fragments arranged by size:
2754 2445 996 815 703 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7873
XbaI T'CTAG_A
Cuts at: 2999 5209 2999
Size: 2210 5663
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2899 3734 4115 2899
Size: 835 381 6657
Fragments arranged by size:
6657 835 381
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7873
XmaI C'CCGG_G
Cuts at: 1870 5198 1870
Size: 3328 4545
XmnI GAAnn'nnTTC
Cuts at: 2128 3031 4959 6002 2128
Size: 903 1928 1043 3999
Fragments arranged by size:
3999 1928 1043 903
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5681 276
Size: 53 83 186 936 4147 2468
Fragments arranged by size:
4147 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BfrBI BsiWI BstXI
EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI