Restriction map for
pFN21AA0255
(Circular) (Six-base) MAPSORT of: pFN21AA0255.seq Check: 3284 from: 1 to: 6580
pFN21AA0255 6580 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2781 2955 4390 278
Size: 53 83 186 936 1245 174 1435 2468
Fragments arranged by size:
2468 1435 1245 936 186 174 83 53
AccI GT'mk_AC
Cuts at: 1937 3345 3923 1937
Size: 1408 578 4594
Fragments arranged by size:
4594 1408 578
Acc65I G'GTAC_C
Cuts at: 2648 3815 3901 2648
Size: 1167 86 5327
Fragments arranged by size:
5327 1167 86
AclI AA'CG_TT
Cuts at: 1563 4707 5080 1563
Size: 3144 373 3063
Fragments arranged by size:
3144 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2941 3502 4643 6027 6295 1632
Size: 1309 561 1141 1384 268 1917
Fragments arranged by size:
1917 1384 1309 1141 561 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6383
AflIII A'CryG_T
Cuts at: 1203 2553 3840 5479 6456 1203
Size: 1350 1287 1639 977 1327
Fragments arranged by size:
1639 1350 1327 1287 977
AgeI A'CCGG_T
Cuts at: 5411 5411
Size: 6580
AhdI GACnn_n'nnGTC
Cuts at: 5309 5309
Size: 6580
AleI CACnn'nnGTG
Cuts at: 1166 3299 1166
Size: 2133 4447
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6580
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 6548
AlwNI CAG_nnn'CTG
Cuts at: 4323 5895 4323
Size: 1572 5008
ApaLI G'TGCA_C
Cuts at: 2632 2942 4637 5793 2632
Size: 310 1695 1156 3419
Fragments arranged by size:
3419 1695 1156 310
ApoI r'AATT_y
Cuts at: 1493 3664 3889 4115 4267 6376 1493
Size: 2171 225 226 152 2109 1697
Fragments arranged by size:
2171 2109 1697 226 225 152
AseI AT'TA_AT
Cuts at: 160 1032 5134 160
Size: 872 4102 1606
Fragments arranged by size:
4102 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
AvaI C'yCGr_G
Cuts at: 1870 1943 3425 3905 1870
Size: 73 1482 480 4545
Fragments arranged by size:
4545 1482 480 73
BamHI G'GATC_C
Cuts at: 3910 4277 3910
Size: 367 6213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2648 3283 3488 3815 3901
Size: 325 217 627 861 635 205 327 86
Cuts at: 3901 5356 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 861 635 627 327 325 217
205 86
BanII G_rGCy'C
Cuts at: 1391 2391 3002 3085 3507 3686 3899 1391
Size: 1000 611 83 422 179 213 4072
Fragments arranged by size:
4072 1000 611 422 213 179 83
BbeI G_GCGC'C
Cuts at: 3492 3492
Size: 6580
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2340 2815 2916 2947 928
Size: 971 441 475 101 31 4561
Fragments arranged by size:
4561 971 475 441 101 31
BbvCI CC'TCA_GC
Cuts at: 2984 2984
Size: 6580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3220 3254 3304 3338 4771 4805 1903
Size: 34 1283 34 50 34 1433 34 3678
Fragments arranged by size:
3678 1433 1283 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2849 3601 4472 5682 2849
Size: 752 871 1210 3747
Fragments arranged by size:
3747 1210 871 752
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6580
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5191 136
Size: 107 122 71 631 605 3519 1525
Fragments arranged by size:
3519 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2481 3855 6575 2481
Size: 1374 2720 2486
Fragments arranged by size:
2720 2486 1374
BlpI GC'TnA_GC
Cuts at: 3997 3997
Size: 6580
Bme1580I G_kGCm'C
Cuts at: 1790 2636 2946 3288 4641 5797 1790
Size: 846 310 342 1353 1156 2573
Fragments arranged by size:
2573 1353 1156 846 342 310
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6580
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2728 2962 4311
Size: 416 53 83 399 24 1311 234 1349
Cuts at: 4311 5269 442
Size: 958 1753
Fragments arranged by size:
1753 1349 1311 958 416 399 234 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6580
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 6580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 2621 3055 5240 1239
Size: 1166 216 434 2185 2579
Fragments arranged by size:
2579 2185 1166 434 216
Bpu10I CC'TnA_GC
Cuts at: 2843 2984 3001 2843
Size: 141 17 6422
Fragments arranged by size:
6422 141 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4705 5570 5868 4705
Size: 865 298 5417
Fragments arranged by size:
5417 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5243 882
Size: 617 3744 2219
Fragments arranged by size:
3744 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2556 4371 493
Size: 711 1352 1815 2702
Fragments arranged by size:
2702 1815 1352 711
BsaBI GATnn'nnATC
Cuts at: 3029 6574 3029
Size: 3545 3035
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2778 2952 3489 4387
Size: 53 83 186 936 1245 174 537 898
Cuts at: 4387 4769 5404 275
Size: 382 635 1451
Fragments arranged by size:
1451 1245 936 898 635 537 382 186
174 83 53
BsaWI w'CCGG_w
Cuts at: 2202 2868 5013 5411 5685 5832 6243 2202
Size: 666 2145 398 274 147 411 2539
Fragments arranged by size:
2539 2145 666 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2540 2570 2915 2945 3524
Size: 30 165 30 1137 30 345 30 579
Cuts at: 3524 3554 1178
Size: 30 4204
Fragments arranged by size:
4204 1137 579 345 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2647 2936 3752 1186
Size: 1461 289 816 4014
Fragments arranged by size:
4014 1461 816 289
BseYI C'CCAG_C
Cuts at: 2109 2150 2751 2995 3470 5783 6279 2109
Size: 41 601 244 475 2313 496 2410
Fragments arranged by size:
2410 2313 601 496 475 244 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2284 2456 2474 3657 1163
Size: 573 548 172 18 1183 4086
Fragments arranged by size:
4086 1183 573 548 172 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4030 4791 5819 1540
Size: 275 186 2029 761 1028 2301
Fragments arranged by size:
2301 2029 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2391 2553 2636 2946 3085 3507 4641 4726 5797
Size: 162 83 310 139 422 1134 85 1071
Cuts at: 5797 2391
Size: 3174
Fragments arranged by size:
3174 1134 1071 422 310 162 139 85
83
BsmI GAATG_Cn'
Cuts at: 3006 3689 4091 4184 3006
Size: 683 402 93 5402
Fragments arranged by size:
5402 683 402 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2391 2553 2636 2946 3002 3085 3288
Size: 399 601 162 83 310 56 83 203
Cuts at: 3288 3507 3686 3899 4641 4726 5797 1391
Size: 219 179 213 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 399 310 219 213
203 179 162 85 83 83 56
BspEI T'CCGG_A
Cuts at: 2868 6243 2868
Size: 3375 3205
BspHI T'CATG_A
Cuts at: 2232 3087 4469 6199 2232
Size: 855 1382 1730 2613
Fragments arranged by size:
2613 1730 1382 855
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2373 2496 3935 844
Size: 1529 123 1439 3489
Fragments arranged by size:
3489 1529 1439 123
BsrBI CCG'CTC
Cuts at: 1394 1823 2323 3281 4467 1394
Size: 429 500 958 1186 3507
Fragments arranged by size:
3507 1186 958 500 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2352 2877 5075 5249 65
Size: 1183 1104 525 2198 174 1396
Fragments arranged by size:
2198 1396 1183 1104 525 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2454 5224 5411 1700
Size: 110 644 2770 187 2869
Fragments arranged by size:
2869 2770 644 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6580
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6580
BssSI C'ACGA_G
Cuts at: 2312 4640 5652 2312
Size: 2328 1012 3240
Fragments arranged by size:
3240 2328 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6580
BstBI TT'CG_AA
Cuts at: 5384 5384
Size: 6580
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6580
BstXI CCAn_nnnn'nTGG
Cuts at: 2383 3301 2383
Size: 918 5662
BstYI r'GATC_y
Cuts at: 1972 2481 2784 3327 3420 3474 3855 3910 4277
Size: 509 303 543 93 54 381 55 367
Cuts at: 4277 4662 4679 6131 6217 6229 6575 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 543 509 385 381 367 346
303 93 86 55 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2643 2643
Size: 6580
BtgI C'CryG_G
Cuts at: 513 1065 2916 3297 513
Size: 552 1851 381 3796
Fragments arranged by size:
3796 1851 552 381
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2424 487
Size: 683 56 1198 4643
Fragments arranged by size:
4643 1198 683 56
BtsI GCAGTG_nn'
Cuts at: 2132 4096 4890 4910 2132
Size: 1964 794 20 3802
Fragments arranged by size:
3802 1964 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6545
DraI TTT'AAA
Cuts at: 3165 3884 3165
Size: 719 5861
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2207 2342 5587 809
Size: 507 891 135 3245 1802
Fragments arranged by size:
3245 1802 891 507 135
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2316 2373 3145
Size: 54 1425 50 275 18 486 57 772
Cuts at: 3145 4027 4916 6545 8
Size: 882 889 1629 43
Fragments arranged by size:
1629 1425 889 882 772 486 275 57
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4027 1537
Size: 275 2215 4090
Fragments arranged by size:
4090 2215 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2532 2618 3113 3512 4510 1353
Size: 1179 86 495 399 998 3423
Fragments arranged by size:
3423 1179 998 495 399 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3147 5167 5541 5687 387
Size: 1064 138 1558 2020 374 146 1280
Fragments arranged by size:
2020 1558 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2389 3083 3505 2389
Size: 694 422 5464
Fragments arranged by size:
5464 694 422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 2621 2941 3055 3502 4643 5240
Size: 393 773 216 320 114 447 1141 597
Cuts at: 5240 6027 6295 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1141 787 773 597 447 393 320
268 216 114
EcoNI CCTnn'n_nnAGG
Cuts at: 2870 2870
Size: 6580
EcoO109I rG'GnC_Cy
Cuts at: 2605 2961 2605
Size: 356 6224
EcoRI G'AATT_C
Cuts at: 3889 4267 3889
Size: 378 6202
EcoRV GAT'ATC
Cuts at: 2734 2734
Size: 6580
FspI TGC'GCA
Cuts at: 3398 5086 3398
Size: 1688 4892
FspAI rTGC'GCAy
Cuts at: 3398 3398
Size: 6580
HaeII r_GCGC'y
Cuts at: 1678 2022 3492 5727 1678
Size: 344 1470 2235 2531
Fragments arranged by size:
2531 2235 1470 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5221 5253 5295 5327 1373
Size: 32 135 32 3649 32 42 32 2626
Fragments arranged by size:
3649 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2212 2476 3727 3924 4170 669
Size: 988 281 274 264 1251 197 246 3079
Fragments arranged by size:
3079 1251 988 281 274 264 246 197
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6580
HpaI GTT'AAC
Cuts at: 3727 4170 3727
Size: 443 6137
KasI G'GCGC_C
Cuts at: 3488 3488
Size: 6580
KpnI G_GTAC'C
Cuts at: 2652 3819 3905 2652
Size: 1167 86 5327
Fragments arranged by size:
5327 1167 86
MfeI C'AATT_G
Cuts at: 4179 4179
Size: 6580
MluI A'CGCG_T
Cuts at: 6456 6456
Size: 6580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 2421 5694 5878 1305
Size: 993 123 3273 184 2007
Fragments arranged by size:
3273 2007 993 184 123
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 10
Size: 54 1425 343 543 4215
Fragments arranged by size:
4215 1425 543 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3116 3122 3299 3464 3584 3614 4538
Size: 648 1950 6 177 165 120 30 924
Cuts at: 4538 4897 5056 518
Size: 359 159 2042
Fragments arranged by size:
2042 1950 924 648 359 177 165 159
120 30 6
MspA1I CmG'CkG
Cuts at: 1606 2695 3013 3433 3631 3651 3996 4673 5821
Size: 1089 318 420 198 20 345 677 1148
Cuts at: 5821 6066 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1089 677 420 345 318 245
198 20
NarI GG'CG_CC
Cuts at: 3489 3489
Size: 6580
NcoI C'CATG_G
Cuts at: 513 1065 2916 3297 513
Size: 552 1851 381 3796
Fragments arranged by size:
3796 1851 552 381
NdeI CA'TA_TG
Cuts at: 387 3382 387
Size: 2995 3585
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6580
NotI GC'GGCC_GC
Cuts at: 4027 4027
Size: 6580
NspI r_CATG'y
Cuts at: 1132 1229 3319 3844 3938 5483 1132
Size: 97 2090 525 94 1545 2229
Fragments arranged by size:
2229 2090 1545 525 97 94
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6580
PciI A'CATG_T
Cuts at: 3840 5479 3840
Size: 1639 4941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6580
PmeI GTTT'AAAC
Cuts at: 3884 3884
Size: 6580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 4649 4681 6162 6194 3524
Size: 32 1093 32 1481 32 3910
Fragments arranged by size:
3910 1481 1093 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2605 2605
Size: 6580
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6580
PsiI TTA'TAA
Cuts at: 4150 4150
Size: 6580
PstI C_TGCA'G
Cuts at: 830 3932 830
Size: 3102 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6580
PvuII CAG'CTG
Cuts at: 2695 3013 3631 2695
Size: 318 618 5644
Fragments arranged by size:
5644 618 318
SacI G_AGCT'C
Cuts at: 2391 3085 3507 2391
Size: 694 422 5464
Fragments arranged by size:
5464 694 422
SalI G'TCGA_C
Cuts at: 1936 3922 1936
Size: 1986 4594
SapI GCTCTTCn'nnn_
Cuts at: 3512 3512
Size: 6580
SbfI CC_TGCA'GG
Cuts at: 3932 3932
Size: 6580
ScaI AGT'ACT
Cuts at: 4828 6540 4828
Size: 1712 4868
SfcI C'TryA_G
Cuts at: 826 1045 3928 5063 5744 5935 826
Size: 219 2883 1135 681 191 1471
Fragments arranged by size:
2883 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3490 3490
Size: 6580
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6580
SmaI CCC'GGG
Cuts at: 1872 3907 1872
Size: 2035 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4684 5585 5847 820
Size: 197 926 2741 901 262 1553
Fragments arranged by size:
2741 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4371 493
Size: 3878 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6580
SphI G_CATG'C
Cuts at: 1132 3938 1132
Size: 2806 3774
SspI AAT'ATT
Cuts at: 5 52 4504 5
Size: 47 4452 2081
Fragments arranged by size:
4452 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6580
StyI C'CwwG_G
Cuts at: 513 1065 2682 2916 3297 4019 513
Size: 552 1617 234 381 722 3074
Fragments arranged by size:
3074 1617 722 552 381 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2919 4607 4624 4777 4962 5407 325
Size: 1255 1339 1688 17 153 185 445 1498
Fragments arranged by size:
1688 1498 1339 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2697 3621 4826
Size: 274 80 33 51 1443 720 924 1205
Cuts at: 4826 6538 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1205 924 720 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2915 2947 6540 6572 2915
Size: 32 3593 32 2923
Fragments arranged by size:
3593 2923 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6580
XbaI T'CTAG_A
Cuts at: 3916 3916
Size: 6580
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6580
XmaI C'CCGG_G
Cuts at: 1870 3905 1870
Size: 2035 4545
XmnI GAAnn'nnTTC
Cuts at: 3251 3539 4709 3251
Size: 288 1170 5122
Fragments arranged by size:
5122 1170 288
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2779 2953 4388 276
Size: 53 83 186 936 1245 174 1435 2468
Fragments arranged by size:
2468 1435 1245 936 186 174 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I DraIII FalI FseI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SrfI SwaI XcmI