Restriction map for
pFN21AA0249
(Circular) (Six-base) MAPSORT of: pFN21AA0249.seq Check: 421 from: 1 to: 7393
pFN21AA0249 7393 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2023 2023
Size: 7393
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5203 278
Size: 53 83 186 936 3667 2468
Fragments arranged by size:
3667 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3150 4736 1937
Size: 1213 1586 4594
Fragments arranged by size:
4594 1586 1213
Acc65I G'GTAC_C
Cuts at: 4714 4714
Size: 7393
AclI AA'CG_TT
Cuts at: 1563 5520 5893 1563
Size: 3957 373 3063
Fragments arranged by size:
3957 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2301 2355 3264 3971 4689 5456 6840 7108
Size: 669 54 909 707 718 767 1384 268
Cuts at: 7108 1632
Size: 1917
Fragments arranged by size:
1917 1384 909 767 718 707 669 268
54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7196
AflIII A'CryG_T
Cuts at: 1203 2419 2543 6292 7269 1203
Size: 1216 124 3749 977 1327
Fragments arranged by size:
3749 1327 1216 977 124
AgeI A'CCGG_T
Cuts at: 6224 6224
Size: 7393
AhdI GACnn_n'nnGTC
Cuts at: 2721 3157 6122 2721
Size: 436 2965 3992
Fragments arranged by size:
3992 2965 436
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4292 1184
Size: 3108 4285
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4568 4600 4568
Size: 32 7361
AlwNI CAG_nnn'CTG
Cuts at: 3389 5136 6708 3389
Size: 1747 1572 4074
Fragments arranged by size:
4074 1747 1572
ApaI G_GGCC'C
Cuts at: 2584 4289 2584
Size: 1705 5688
ApaLI G'TGCA_C
Cuts at: 5450 6606 5450
Size: 1156 6237
ApoI r'AATT_y
Cuts at: 1493 3450 3464 3494 4609 4702 4928 5080 7189
Size: 1957 14 30 1115 93 226 152 2109
Cuts at: 7189 1493
Size: 1697
Fragments arranged by size:
2109 1957 1697 1115 226 152 93 30
14
AseI AT'TA_AT
Cuts at: 160 1032 4517 5947 160
Size: 872 3485 1430 1606
Fragments arranged by size:
3485 1606 1430 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7393
AvaI C'yCGr_G
Cuts at: 1870 1943 3302 3817 4281 4718 1870
Size: 73 1359 515 464 437 4545
Fragments arranged by size:
4545 1359 515 464 437 73
AvrII C'CTAG_G
Cuts at: 2858 2858
Size: 7393
BamHI G'GATC_C
Cuts at: 4723 5090 4723
Size: 367 7026
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3118 3368 3745 3791 4007
Size: 325 217 627 1331 250 377 46 216
Cuts at: 4007 4714 6169 618
Size: 707 1455 1842
Fragments arranged by size:
1842 1455 1331 707 627 377 325 250
217 216 46
BanII G_rGCy'C
Cuts at: 1391 2564 2584 2998 3285 3843 3882 4289 4572
Size: 1173 20 414 287 558 39 407 283
Cuts at: 4572 4712 1391
Size: 140 4072
Fragments arranged by size:
4072 1173 558 414 407 287 283 140
39 20
BbeI G_GCGC'C
Cuts at: 3122 3122
Size: 7393
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2544 2964 928
Size: 971 645 420 5357
Fragments arranged by size:
5357 971 645 420
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5584 5618 1903
Size: 34 3647 34 3678
Fragments arranged by size:
3678 3647 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5285 6495 5285
Size: 1210 6183
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7393
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3800 6004 136
Size: 107 122 71 631 605 2128 2204 1525
Fragments arranged by size:
2204 2128 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7388 7388
Size: 7393
BlpI GC'TnA_GC
Cuts at: 4810 4810
Size: 7393
Bme1580I G_kGCm'C
Cuts at: 1790 2584 4271 4289 5454 6610 1790
Size: 794 1687 18 1165 1156 2573
Fragments arranged by size:
2573 1687 1165 1156 794 18
BmgBI CAC'GTC
Cuts at: 1318 2422 2799 1318
Size: 1104 377 5912
Fragments arranged by size:
5912 1104 377
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2904 3322 3574
Size: 416 53 83 399 24 1487 418 252
Cuts at: 3574 3991 4261 5124 6082 442
Size: 417 270 863 958 1753
Fragments arranged by size:
1753 1487 958 863 418 417 416 399
270 252 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7393
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3139 3397 3829 4654 3139
Size: 258 432 825 5878
Fragments arranged by size:
5878 825 432 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2765 6053 1239
Size: 1526 3288 2579
Fragments arranged by size:
3288 2579 1526
Bpu10I CC'TnA_GC
Cuts at: 3109 3590 3884 3109
Size: 481 294 6618
Fragments arranged by size:
6618 481 294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3074 5518 6383 6681 3074
Size: 2444 865 298 3786
Fragments arranged by size:
3786 2444 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2693 4650 6056 882
Size: 617 1194 1957 1406 2219
Fragments arranged by size:
2219 1957 1406 1194 617
BsaAI yAC'GTr
Cuts at: 493 1204 5184 493
Size: 711 3980 2702
Fragments arranged by size:
3980 2702 711
BsaBI GATnn'nnATC
Cuts at: 7387 7387
Size: 7393
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3119 5200 5582 6217
Size: 53 83 186 936 1586 2081 382 635
Cuts at: 6217 275
Size: 1451
Fragments arranged by size:
2081 1586 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5826 6224 6498 6645 7056 5826
Size: 398 274 147 411 6163
Fragments arranged by size:
6163 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2783 2813 3939 3969 4098
Size: 30 165 30 1380 30 1126 30 129
Cuts at: 4098 4128 4568 4598 1178
Size: 30 440 30 3973
Fragments arranged by size:
3973 1380 1126 440 165 129 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 3039 3805 3919 1186
Size: 1102 751 766 114 4660
Fragments arranged by size:
4660 1102 766 751 114
BseYI C'CCAG_C
Cuts at: 2027 2167 4123 4304 6596 7092 2027
Size: 140 1956 181 2292 496 2328
Fragments arranged by size:
2328 2292 1956 496 181 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2241 4041 4232 1163
Size: 573 505 1800 191 4324
Fragments arranged by size:
4324 1800 573 505 191
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3789 3804 4843 5604 6632 1540
Size: 275 186 1788 15 1039 761 1028 2301
Fragments arranged by size:
2301 1788 1039 1028 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2564 3843 4572 5454 5539 6610 2564
Size: 1279 729 882 85 1071 3347
Fragments arranged by size:
3347 1279 1071 882 729 85
BsmI GAATG_Cn'
Cuts at: 4904 4997 4904
Size: 93 7300
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2564 2584 2998 3285 3843 3882 4271
Size: 399 774 20 414 287 558 39 389
Cuts at: 4271 4289 4572 4712 5454 5539 6610 1391
Size: 18 283 140 742 85 1071 2174
Fragments arranged by size:
2174 1071 774 742 558 414 399 389
287 283 140 85 39 20 18
BspEI T'CCGG_A
Cuts at: 7056 7056
Size: 7393
BspHI T'CATG_A
Cuts at: 5282 7012 5282
Size: 1730 5663
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2023 2764 3771 4254 4748 844
Size: 1179 741 1007 483 494 3489
Fragments arranged by size:
3489 1179 1007 741 494 483
BsrBI CCG'CTC
Cuts at: 1394 1823 5280 1394
Size: 429 3457 3507
Fragments arranged by size:
3507 3457 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5888 6062 65
Size: 1183 4640 174 1396
Fragments arranged by size:
4640 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3136 3784 3788 3799 6037 6224 1700
Size: 110 1326 648 4 11 2238 187 2869
Fragments arranged by size:
2869 2238 1326 648 187 110 11 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7393
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7393
BssSI C'ACGA_G
Cuts at: 2592 4209 4571 5453 6465 2592
Size: 1617 362 882 1012 3520
Fragments arranged by size:
3520 1617 1012 882 362
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2528 3375 794
Size: 1734 847 4812
Fragments arranged by size:
4812 1734 847
BstBI TT'CG_AA
Cuts at: 6197 6197
Size: 7393
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7393
BstXI CCAn_nnnn'nTGG
Cuts at: 2998 3859 4222 2998
Size: 861 363 6169
Fragments arranged by size:
6169 861 363
BstYI r'GATC_y
Cuts at: 1972 2180 2227 2597 4723 5090 5475 5492 6944
Size: 208 47 370 2126 367 385 17 1452
Cuts at: 6944 7030 7042 7388 1972
Size: 86 12 346 1977
Fragments arranged by size:
2126 1977 1452 385 370 367 346 208
86 47 17 12
Bsu36I CC'TnA_GG
Cuts at: 3621 3621
Size: 7393
BtgI C'CryG_G
Cuts at: 513 1065 2952 3342 3393 3889 4237 513
Size: 552 1887 390 51 496 348 3669
Fragments arranged by size:
3669 1887 552 496 390 348 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6654
Fragments arranged by size:
6654 683 56
BtsI GCAGTG_nn'
Cuts at: 2224 2229 3302 4909 5703 5723 2224
Size: 5 1073 1607 794 20 3894
Fragments arranged by size:
3894 1607 1073 794 20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7393
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3009 3044 529
Size: 35 2445 35 4878
Fragments arranged by size:
4878 2445 35 35
DraI TTT'AAA
Cuts at: 2350 4697 2350
Size: 2347 5046
DraIII CAC_nnn'GTG
Cuts at: 3375 3375
Size: 7393
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6400 809
Size: 507 5084 1802
Fragments arranged by size:
5084 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3786 3801 4224
Size: 54 1425 50 275 18 1956 15 423
Cuts at: 4224 4840 5729 7358 8
Size: 616 889 1629 43
Fragments arranged by size:
1956 1629 1425 889 616 423 275 54
50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3786 3801 4840 1537
Size: 275 1974 15 1039 4090
Fragments arranged by size:
4090 1974 1039 275 15
EarI CTCTTCn'nnn_
Cuts at: 1353 2505 2651 3605 5323 1353
Size: 1152 146 954 1718 3423
Fragments arranged by size:
3423 1718 1152 954 146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2820 3911 4309 5980 6354 6500
Size: 1064 138 1231 1091 398 1671 374 146
Cuts at: 6500 387
Size: 1280
Fragments arranged by size:
1671 1280 1231 1091 1064 398 374 146
138
EcoICRI GAG'CTC
Cuts at: 2562 3841 4570 2562
Size: 1279 729 5385
Fragments arranged by size:
5385 1279 729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2355 2765 3264 3971 4689 5456
Size: 393 669 54 410 499 707 718 767
Cuts at: 5456 6053 6840 7108 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 767 718 707 669 597 499
410 393 268 54
EcoO109I rG'GnC_Cy
Cuts at: 2580 3200 3430 4285 4286 2580
Size: 620 230 855 1 5687
Fragments arranged by size:
5687 855 620 230 1
EcoRI G'AATT_C
Cuts at: 4609 4702 5080 4609
Size: 93 378 6922
Fragments arranged by size:
6922 378 93
EcoRV GAT'ATC
Cuts at: 4384 4384
Size: 7393
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4432 4432
Size: 7393
FseI GG_CCGG'CC
Cuts at: 3803 3803
Size: 7393
FspI TGC'GCA
Cuts at: 5899 5899
Size: 7393
HaeII r_GCGC'y
Cuts at: 1678 3122 6540 1678
Size: 1444 3418 2531
Fragments arranged by size:
3418 2531 1444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2692 2707 2724 2739 3008
Size: 32 135 32 1120 15 17 15 269
Cuts at: 3008 3040 3143 3175 3302 3334 3535 3567 3722
Size: 32 103 32 127 32 201 32 155
Cuts at: 3722 3752 3754 3784 3815 3847 4098 4130 4647
Size: 30 2 30 31 32 251 32 517
Cuts at: 4647 4673 4679 4705 6034 6066 6108 6140 1373
Size: 26 6 26 1329 32 42 32 2626
Fragments arranged by size:
2626 1329 1120 517 269 251 201 155
135 127 103 42 32 32 32 32
32 32 32 32 32 32 31 30
30 26 26 17 15 15 6 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4737 4983 669
Size: 988 281 2799 246 3079
Fragments arranged by size:
3079 2799 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2259 2297 748
Size: 1511 38 5844
Fragments arranged by size:
5844 1511 38
HpaI GTT'AAC
Cuts at: 4983 4983
Size: 7393
KasI G'GCGC_C
Cuts at: 3118 3118
Size: 7393
KpnI G_GTAC'C
Cuts at: 4718 4718
Size: 7393
MfeI C'AATT_G
Cuts at: 4992 4992
Size: 7393
MluI A'CGCG_T
Cuts at: 7269 7269
Size: 7393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4074 4450 6507 6691 1305
Size: 2769 376 2057 184 2007
Fragments arranged by size:
2769 2057 2007 376 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5571
Fragments arranged by size:
5571 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3857 4220 5351 5710 5869 518
Size: 648 2691 363 1131 359 159 2042
Fragments arranged by size:
2691 2042 1131 648 363 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 3356 3715 4123 4809 5486 6634 6879
Size: 417 1333 359 408 686 677 1148 245
Cuts at: 6879 1606
Size: 2120
Fragments arranged by size:
2120 1333 1148 686 677 417 408 359
245
NaeI GCC'GGC
Cuts at: 3786 3801 3786
Size: 15 7378
NarI GG'CG_CC
Cuts at: 3119 3119
Size: 7393
NcoI C'CATG_G
Cuts at: 513 1065 2952 3393 513
Size: 552 1887 441 4513
Fragments arranged by size:
4513 1887 552 441
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7393
NgoMIV G'CCGG_C
Cuts at: 3784 3799 3784
Size: 15 7378
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7393
NotI GC'GGCC_GC
Cuts at: 4840 4840
Size: 7393
NspI r_CATG'y
Cuts at: 1132 1229 2547 4583 4751 6296 1132
Size: 97 1318 2036 168 1545 2229
Fragments arranged by size:
2229 2036 1545 1318 168 97
PasI CC'CwG_GG
Cuts at: 1348 2998 1348
Size: 1650 5743
PciI A'CATG_T
Cuts at: 2543 6292 2543
Size: 3749 3644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3297 1108
Size: 2189 5204
PmeI GTTT'AAAC
Cuts at: 4697 4697
Size: 7393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4568 4600 5462 5494 6975 7007 4568
Size: 32 862 32 1481 32 4954
Fragments arranged by size:
4954 1481 862 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3200 3200
Size: 7393
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7393
PsiI TTA'TAA
Cuts at: 4963 4963
Size: 7393
PspOMI G'GGCC_C
Cuts at: 2580 4285 2580
Size: 1705 5688
PspXI vC'TCGA_Gb
Cuts at: 3817 3817
Size: 7393
PstI C_TGCA'G
Cuts at: 830 2536 3361 4745 830
Size: 1706 825 1384 3478
Fragments arranged by size:
3478 1706 1384 825
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7393
PvuII CAG'CTG
Cuts at: 2023 3356 3715 4123 2023
Size: 1333 359 408 5293
Fragments arranged by size:
5293 1333 408 359
SacI G_AGCT'C
Cuts at: 2564 3843 4572 2564
Size: 1279 729 5385
Fragments arranged by size:
5385 1279 729
SalI G'TCGA_C
Cuts at: 1936 4735 1936
Size: 2799 4594
SapI GCTCTTCn'nnn_
Cuts at: 2505 2651 3605 2505
Size: 146 954 6293
Fragments arranged by size:
6293 954 146
SbfI CC_TGCA'GG
Cuts at: 4745 4745
Size: 7393
ScaI AGT'ACT
Cuts at: 3061 5641 7353 3061
Size: 2580 1712 3101
Fragments arranged by size:
3101 2580 1712
SfcI C'TryA_G
Cuts at: 826 1045 2532 3357 4483 4741 5876 6557 6748
Size: 219 1487 825 1126 258 1135 681 191
Cuts at: 6748 826
Size: 1471
Fragments arranged by size:
1487 1471 1135 1126 825 681 258 219
191
SfoI GGC'GCC
Cuts at: 3120 3120
Size: 7393
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7393
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7393
SmaI CCC'GGG
Cuts at: 1872 4283 4720 1872
Size: 2411 437 4545
Fragments arranged by size:
4545 2411 437
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3053 3817 5497 6398 6660 820
Size: 197 926 1110 764 1680 901 262 1553
Fragments arranged by size:
1680 1553 1110 926 901 764 262 197
SnaBI TAC'GTA
Cuts at: 493 5184 493
Size: 4691 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7393
SphI G_CATG'C
Cuts at: 1132 4751 1132
Size: 3619 3774
SspI AAT'ATT
Cuts at: 5 52 4491 5317 5
Size: 47 4439 826 2081
Fragments arranged by size:
4439 2081 826 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7393
StyI C'CwwG_G
Cuts at: 513 1065 2823 2858 2952 3393 3726 3777 4506
Size: 552 1758 35 94 441 333 51 729
Cuts at: 4506 4832 513
Size: 326 3074
Fragments arranged by size:
3074 1758 729 552 441 333 326 94
51 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2079 2234 3631 5420 5437 5590 5775
Size: 1255 499 155 1397 1789 17 153 185
Cuts at: 5775 6220 325
Size: 445 1498
Fragments arranged by size:
1789 1498 1397 1255 499 445 185 155
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3059 4197 5639
Size: 274 80 33 51 1443 1082 1138 1442
Cuts at: 5639 7351 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1442 1138 1082 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2783 2815 7353 7385 2783
Size: 32 4538 32 2791
Fragments arranged by size:
4538 2791 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2963 1316
Size: 1647 5746
XbaI T'CTAG_A
Cuts at: 3235 4729 3235
Size: 1494 5899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 7393
XhoI C'TCGA_G
Cuts at: 1943 3817 1943
Size: 1874 5519
XmaI C'CCGG_G
Cuts at: 1870 4281 4718 1870
Size: 2411 437 4545
Fragments arranged by size:
4545 2411 437
XmnI GAAnn'nnTTC
Cuts at: 2298 5522 2298
Size: 3224 4169
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5201 276
Size: 53 83 186 936 3667 2468
Fragments arranged by size:
3667 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I
EcoNI FspAI NruI NsiI PacI PfoI PmlI PsrI
RsrII SacII SanDI SexAI SfiI SrfI SwaI