Restriction map for
pFN21AA0236
(Circular) (Six-base) MAPSORT of: pFN21AA0236.seq Check: 7375 from: 1 to: 9766
pFN21AA0236 9766 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2556 4375 4665 4795 7005 2556
Size: 1819 290 130 2210 5317
Fragments arranged by size:
5317 2210 1819 290 130
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7576 278
Size: 53 83 186 936 6040 2468
Fragments arranged by size:
6040 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7109 1937
Size: 5172 4594
Acc65I G'GTAC_C
Cuts at: 4745 5640 5717 7087 4745
Size: 895 77 1370 7424
Fragments arranged by size:
7424 1370 895 77
AclI AA'CG_TT
Cuts at: 1563 7893 8266 1563
Size: 6330 373 3063
Fragments arranged by size:
6330 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2223 2379 2945 3736 4281 4682 5072 5163
Size: 591 156 566 791 545 401 390 91
Cuts at: 5163 6207 6623 7829 9213 9481 1632
Size: 1044 416 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1206 1044 791 591 566 545
416 401 390 268 156 91
AfeI AGC'GCT
Cuts at: 2513 2513
Size: 9766
AflII C'TTAA_G
Cuts at: 820 1017 5452 5906 820
Size: 197 4435 454 4680
Fragments arranged by size:
4680 4435 454 197
AflIII A'CryG_T
Cuts at: 1203 3375 5327 8665 9642 1203
Size: 2172 1952 3338 977 1327
Fragments arranged by size:
3338 2172 1952 1327 977
AgeI A'CCGG_T
Cuts at: 5643 5742 8597 5643
Size: 99 2855 6812
Fragments arranged by size:
6812 2855 99
AhdI GACnn_n'nnGTC
Cuts at: 8495 8495
Size: 9766
AleI CACnn'nnGTG
Cuts at: 1166 2682 3294 1166
Size: 1516 612 7638
Fragments arranged by size:
7638 1516 612
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4673 5499 1184
Size: 3489 826 5451
Fragments arranged by size:
5451 3489 826
AlwNI CAG_nnn'CTG
Cuts at: 2201 2246 2446 3970 4292 4883 5421 7509 9081
Size: 45 200 1524 322 591 538 2088 1572
Cuts at: 9081 2201
Size: 2886
Fragments arranged by size:
2886 2088 1572 1524 591 538 322 200
45
ApaI G_GGCC'C
Cuts at: 3866 5505 5984 3866
Size: 1639 479 7648
Fragments arranged by size:
7648 1639 479
ApaLI G'TGCA_C
Cuts at: 3379 3425 6364 6662 6992 7823 8979 3379
Size: 46 2939 298 330 831 1156 4166
Fragments arranged by size:
4166 2939 1156 831 330 298 46
ApoI r'AATT_y
Cuts at: 1493 2786 7075 7301 7453 9562 1493
Size: 1293 4289 226 152 2109 1697
Fragments arranged by size:
4289 2109 1697 1293 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8320 160
Size: 872 7288 1606
Fragments arranged by size:
7288 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9766
AvaI C'yCGr_G
Cuts at: 1870 1943 2142 4610 5390 5933 6171 6620 7091
Size: 73 199 2468 780 543 238 449 471
Cuts at: 7091 1870
Size: 4545
Fragments arranged by size:
4545 2468 780 543 471 449 238 199
73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6509 6542 6509
Size: 33 9733
BamHI G'GATC_C
Cuts at: 6504 7018 7096 7463 6504
Size: 514 78 367 8807
Fragments arranged by size:
8807 514 367 78
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2468 4578 4745 5475 5640
Size: 325 217 627 681 2110 167 730 165
Cuts at: 5640 5717 5849 6930 7087 8542 618
Size: 77 132 1081 157 1455 1842
Fragments arranged by size:
2110 1842 1455 1081 730 681 627 325
217 167 165 157 132 77
BanII G_rGCy'C
Cuts at: 1391 2132 2440 3866 3891 4299 4599 5311 5359
Size: 741 308 1426 25 408 300 712 48
Cuts at: 5359 5505 5984 7085 1391
Size: 146 479 1101 4072
Fragments arranged by size:
4072 1426 1101 741 712 479 408 308
300 146 48 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4009 928
Size: 971 2110 6685
Fragments arranged by size:
6685 2110 971
BbvCI CC'TCA_GC
Cuts at: 2795 4646 2795
Size: 1851 7915
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7957 7991 1903
Size: 34 6020 34 3678
Fragments arranged by size:
6020 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5272 7658 8868 5272
Size: 2386 1210 6170
Fragments arranged by size:
6170 2386 1210
BclI T'GATC_A
Cuts at: 1548 3496 1548
Size: 1948 7818
BfrBI ATG'CAT
Cuts at: 2728 3187 2728
Size: 459 9307
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3005 3105 5847
Size: 107 122 71 631 605 1333 100 2742
Cuts at: 5847 8377 136
Size: 2530 1525
Fragments arranged by size:
2742 2530 1525 1333 631 605 122 107
100 71
BglII A'GATC_T
Cuts at: 9761 9761
Size: 9766
BlpI GC'TnA_GC
Cuts at: 2439 3503 3683 7183 2439
Size: 1064 180 3500 5022
Fragments arranged by size:
5022 3500 1064 180
Bme1580I G_kGCm'C
Cuts at: 1790 2069 2585 3383 3429 3733 3866 4610 4673
Size: 279 516 798 46 304 133 744 63
Cuts at: 4673 5505 5612 5984 6017 6350 6368 6536 6666
Size: 832 107 372 33 333 18 168 130
Cuts at: 6666 6996 7827 8983 1790
Size: 330 831 1156 2573
Fragments arranged by size:
2573 1156 832 831 798 744 516 372
333 330 304 279 168 133 130 107
63 46 33 18
BmgBI CAC'GTC
Cuts at: 1318 3325 5162 1318
Size: 2007 1837 5922
Fragments arranged by size:
5922 2007 1837
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3149 4348 4584
Size: 416 53 83 399 24 1732 1199 236
Cuts at: 4584 4886 4946 5006 5866 5878 6438 7497 8455
Size: 302 60 60 860 12 560 1059 958
Cuts at: 8455 442
Size: 1753
Fragments arranged by size:
1753 1732 1199 1059 958 860 560 416
399 302 236 83 60 60 53 24
12
BmtI G_CTAG'C
Cuts at: 1056 4626 1056
Size: 3570 6196
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5281 5281
Size: 9766
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 2292 2561 3825 4136 4552 5421 7034
Size: 1044 9 269 1264 311 416 869 1613
Cuts at: 7034 8426 1239
Size: 1392 2579
Fragments arranged by size:
2579 1613 1392 1264 1044 869 416 311
269 9
Bpu10I CC'TnA_GC
Cuts at: 2070 2795 4646 2070
Size: 725 1851 7190
Fragments arranged by size:
7190 1851 725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2606 2804 4160 4400 5564 6086 6473 7891 8756
Size: 198 1356 240 1164 522 387 1418 865
Cuts at: 8756 9054 2606
Size: 298 3318
Fragments arranged by size:
3318 1418 1356 1164 865 522 387 298
240 198
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2270 2467 2924 3801 5963 6665 8429
Size: 617 771 197 457 877 2162 702 1764
Cuts at: 8429 882
Size: 2219
Fragments arranged by size:
2219 2162 1764 877 771 702 617 457
197
BsaAI yAC'GTr
Cuts at: 493 1204 5137 6922 7557 493
Size: 711 3933 1785 635 2702
Fragments arranged by size:
3933 2702 1785 711 635
BsaBI GATnn'nnATC
Cuts at: 9760 9760
Size: 9766
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7573 7955 8590 275
Size: 53 83 186 936 6040 382 635 1451
Fragments arranged by size:
6040 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5643 5742 6120 8199 8597 8871 9018 9429 5643
Size: 99 378 2079 398 274 147 411 5980
Fragments arranged by size:
5980 2079 411 398 378 274 147 99
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2269 2299 3458 3488 4294
Size: 30 165 30 866 30 1159 30 806
Cuts at: 4294 4324 4722 4752 5075 5105 1178
Size: 30 398 30 323 30 5839
Fragments arranged by size:
5839 1159 866 806 398 323 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 4001 4085 4157 4337 4963 5023 5110
Size: 1192 1623 84 72 180 626 60 87
Cuts at: 5110 5408 6230 1186
Size: 298 822 4722
Fragments arranged by size:
4722 1623 1192 822 626 298 180 87
84 72 60
BseYI C'CCAG_C
Cuts at: 2281 2570 2644 3603 3953 4052 4057 5196 5491
Size: 289 74 959 350 99 5 1139 295
Cuts at: 5491 5854 6017 6813 7022 8969 9465 2281
Size: 363 163 796 209 1947 496 2582
Fragments arranged by size:
2582 1947 1139 959 796 496 363 350
295 289 209 163 99 74 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2136 3109 4066 4496 4547 5393 5925
Size: 573 400 973 957 430 51 846 532
Cuts at: 5925 5972 6619 7028 1163
Size: 47 647 409 3901
Fragments arranged by size:
3901 973 957 846 647 573 532 430
409 400 51 47
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2902 6856 7216 7977 9005 1540
Size: 275 186 901 3954 360 761 1028 2301
Fragments arranged by size:
3954 2301 1028 901 761 360 275 186
BsiHKAI G_wGCw'C
Cuts at: 2269 2547 2817 3252 3383 3429 3476 4740 6120
Size: 278 270 435 131 46 47 1264 1380
Cuts at: 6120 6368 6666 6996 7827 7912 8983 2269
Size: 248 298 330 831 85 1071 3052
Fragments arranged by size:
3052 1380 1264 1071 831 435 330 298
278 270 248 131 85 47 46
BsiWI C'GTAC_G
Cuts at: 6922 6922
Size: 9766
BsmI GAATG_Cn'
Cuts at: 2704 7277 7370 2704
Size: 4573 93 5100
Fragments arranged by size:
5100 4573 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3315 6359 6491 3315
Size: 3044 132 6590
Fragments arranged by size:
6590 3044 132
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2132 2269 2440 2547 2585 2817
Size: 399 279 63 137 171 107 38 232
Cuts at: 2817 3252 3383 3429 3476 3733 3866 3891 4299
Size: 435 131 46 47 257 133 25 408
Cuts at: 4299 4599 4610 4673 4740 5311 5359 5505 5612
Size: 300 11 63 67 571 48 146 107
Cuts at: 5612 5984 6017 6120 6350 6368 6536 6666 6996
Size: 372 33 103 230 18 168 130 330
Cuts at: 6996 7085 7827 7912 8983 1391
Size: 89 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 571 435 408 399 372
330 300 279 257 232 230 171 168
146 137 133 131 130 107 107 103
89 85 67 63 63 48 47 46
38 33 25 18 11
BspEI T'CCGG_A
Cuts at: 9429 9429
Size: 9766
BspHI T'CATG_A
Cuts at: 3193 7655 9385 3193
Size: 4462 1730 3574
Fragments arranged by size:
4462 3574 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2318 2556 4375 4665 4795 7005 7121 844
Size: 1474 238 1819 290 130 2210 116 3489
Fragments arranged by size:
3489 2210 1819 1474 290 238 130 116
BsrBI CCG'CTC
Cuts at: 1394 1823 6178 7043 7653 1394
Size: 429 4355 865 610 3507
Fragments arranged by size:
4355 3507 865 610 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5227 6796 8261 8435 65
Size: 1183 3979 1569 1465 174 1396
Fragments arranged by size:
3979 1569 1465 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5571 5643 5742 8410 8597 1700
Size: 110 3761 72 99 2668 187 2869
Fragments arranged by size:
3761 2869 2668 187 110 99 72
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9766
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9766
BssSI C'ACGA_G
Cuts at: 5585 7826 8838 5585
Size: 2241 1012 6513
Fragments arranged by size:
6513 2241 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3132 3471 3679 4735 5094 5421 5691 5700
Size: 2338 339 208 1056 359 327 270 9
Cuts at: 5700 5920 5948 6795 7006 794
Size: 220 28 847 211 3554
Fragments arranged by size:
3554 2338 1056 847 359 339 327 270
220 211 208 28 9
BstBI TT'CG_AA
Cuts at: 8570 8570
Size: 9766
BstEII G'GTnAC_C
Cuts at: 1184 4034 1184
Size: 2850 6916
BstXI CCAn_nnnn'nTGG
Cuts at: 5714 5714
Size: 9766
BstYI r'GATC_y
Cuts at: 1972 3201 5373 6504 7018 7096 7463 7848 7865
Size: 1229 2172 1131 514 78 367 385 17
Cuts at: 7865 9317 9403 9415 9761 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2172 1977 1452 1229 1131 514 385 367
346 86 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 3452 3915 4424 7059 3452
Size: 463 509 2635 6159
Fragments arranged by size:
6159 2635 509 463
BtgI C'CryG_G
Cuts at: 513 1065 2104 2935 3000 3790 4027 4980 5497
Size: 552 1039 831 65 790 237 953 517
Cuts at: 5497 6059 6231 513
Size: 562 172 4048
Fragments arranged by size:
4048 1039 953 831 790 562 552 517
237 172 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9027
Fragments arranged by size:
9027 683 56
BtsI GCAGTG_nn'
Cuts at: 2662 3034 3115 3231 4021 4522 4939 5083 5410
Size: 372 81 116 790 501 417 144 327
Cuts at: 5410 5430 5445 5481 6277 6653 6716 7282 8076
Size: 20 15 36 796 376 63 566 794
Cuts at: 8076 8096 2662
Size: 20 4332
Fragments arranged by size:
4332 796 794 790 566 501 417 376
372 327 144 116 81 63 36 20
20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9766
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9731
DraI TTT'AAA
Cuts at: 7070 7070
Size: 9766
DraIII CAC_nnn'GTG
Cuts at: 3405 5789 6331 6659 3405
Size: 2384 542 328 6512
Fragments arranged by size:
6512 2384 542 328
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8773 809
Size: 507 7457 1802
Fragments arranged by size:
7457 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2718 5680 6262
Size: 54 1425 50 275 18 888 2962 582
Cuts at: 6262 7213 8102 9731 8
Size: 951 889 1629 43
Fragments arranged by size:
2962 1629 1425 951 889 888 582 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7213 1537
Size: 275 5401 4090
Fragments arranged by size:
5401 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 2355 2376 2381 2486 3108 4857 7696
Size: 846 156 21 5 105 622 1749 2839
Cuts at: 7696 1353
Size: 3423
Fragments arranged by size:
3423 2839 1749 846 622 156 105 21
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2780 8353 8727 8873 387
Size: 1064 138 1191 5573 374 146 1280
Fragments arranged by size:
5573 1280 1191 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 2283 2292 2379 2561 2945 3736
Size: 393 591 60 9 87 182 384 791
Cuts at: 3736 3825 4136 4281 4552 4682 5072 5163 5421
Size: 89 311 145 271 130 390 91 258
Cuts at: 5421 6207 6623 7034 7829 8426 9213 9481 1239
Size: 786 416 411 795 597 787 268 1524
Fragments arranged by size:
1524 795 791 787 786 597 591 416
411 393 390 384 311 271 268 258
182 145 130 91 89 87 60 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2798 4142 4902 4962 5022 2798
Size: 1344 760 60 60 7542
Fragments arranged by size:
7542 1344 760 60 60
EcoO109I rG'GnC_Cy
Cuts at: 2117 2146 2803 3448 3862 3967 4099 4176 4420
Size: 29 657 645 414 105 132 77 244
Cuts at: 4420 4552 4927 4987 5047 5184 5502 5980 5981
Size: 132 375 60 60 137 318 478 1
Cuts at: 5981 6075 2117
Size: 94 5808
Fragments arranged by size:
5808 657 645 478 414 375 318 244
137 132 132 105 94 77 60 60
29 1
EcoRI G'AATT_C
Cuts at: 2786 7075 7453 2786
Size: 4289 378 5099
Fragments arranged by size:
5099 4289 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 4886 4960 5080 6751 2533
Size: 2353 74 120 1671 5548
Fragments arranged by size:
5548 2353 1671 120 74
FspI TGC'GCA
Cuts at: 6746 8272 6746
Size: 1526 8240
HaeII r_GCGC'y
Cuts at: 1678 2515 8913 1678
Size: 837 6398 2531
Fragments arranged by size:
6398 2531 837
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2119 2151 2269 2301 2447
Size: 32 135 32 547 32 118 32 146
Cuts at: 2447 2479 3175 3207 4103 4135 4391 4423 6387
Size: 32 696 32 896 32 256 32 1964
Cuts at: 6387 6419 6664 6696 8407 8439 8481 8513 1373
Size: 32 245 32 1711 32 42 32 2626
Fragments arranged by size:
2626 1964 1711 896 696 547 256 245
146 135 118 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3994 5833 6565 7110 7356 669
Size: 988 281 2056 1839 732 545 246 3079
Fragments arranged by size:
3079 2056 1839 988 732 545 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9766
HpaI GTT'AAC
Cuts at: 7356 7356
Size: 9766
KpnI G_GTAC'C
Cuts at: 4749 5644 5721 7091 4749
Size: 895 77 1370 7424
Fragments arranged by size:
7424 1370 895 77
MfeI C'AATT_G
Cuts at: 7365 7365
Size: 9766
MluI A'CGCG_T
Cuts at: 9642 9642
Size: 9766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2766 3280 3384 8880 9064 1305
Size: 1461 514 104 5496 184 2007
Fragments arranged by size:
5496 2007 1461 514 184 104
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2720 6264 10
Size: 54 1425 343 888 3544 3512
Fragments arranged by size:
3544 3512 1425 888 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2682 3192 3276 3294 3795 4032 6613
Size: 648 1516 510 84 18 501 237 2581
Cuts at: 6613 6999 7724 8083 8242 518
Size: 386 725 359 159 2042
Fragments arranged by size:
2581 2042 1516 725 648 510 501 386
359 237 159 84 18
MspA1I CmG'CkG
Cuts at: 1606 2357 2443 3502 3901 4080 4515 5838 6000
Size: 751 86 1059 399 179 435 1323 162
Cuts at: 6000 6147 6313 6571 6813 7012 7182 7859 9007
Size: 147 166 258 242 199 170 677 1148
Cuts at: 9007 9252 1606
Size: 245 2120
Fragments arranged by size:
2120 1323 1148 1059 751 677 435 399
258 245 242 199 179 170 166 162
147 86
NcoI C'CATG_G
Cuts at: 513 1065 2935 3000 3790 4027 513
Size: 552 1870 65 790 237 6252
Fragments arranged by size:
6252 1870 790 552 237 65
NdeI CA'TA_TG
Cuts at: 387 2493 387
Size: 2106 7660
NheI G'CTAG_C
Cuts at: 1052 4622 1052
Size: 3570 6196
NotI GC'GGCC_GC
Cuts at: 7213 7213
Size: 9766
NsiI A_TGCA'T
Cuts at: 2730 3189 2730
Size: 459 9307
NspI r_CATG'y
Cuts at: 1132 1229 2728 3191 3265 3318 3349 3379 3433
Size: 97 1499 463 74 53 31 30 54
Cuts at: 3433 3443 5331 5563 5918 6303 6502 7124 8669
Size: 10 1888 232 355 385 199 622 1545
Cuts at: 8669 1132
Size: 2229
Fragments arranged by size:
2229 1888 1545 1499 622 463 385 355
232 199 97 74 54 53 31 30
10
PasI CC'CwG_GG
Cuts at: 1348 4102 4180 4842 1348
Size: 2754 78 662 6272
Fragments arranged by size:
6272 2754 662 78
PciI A'CATG_T
Cuts at: 3375 5327 8665 3375
Size: 1952 3338 4476
Fragments arranged by size:
4476 3338 1952
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5498 6313 6964 1108
Size: 4390 815 651 3910
Fragments arranged by size:
4390 3910 815 651
PfoI T'CCnGG_A
Cuts at: 2269 2299 4568 2269
Size: 30 2269 7467
Fragments arranged by size:
7467 2269 30
PmeI GTTT'AAAC
Cuts at: 7070 7070
Size: 9766
PmlI CAC'GTG
Cuts at: 5137 5137
Size: 9766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7835 7867 9348 9380 7835
Size: 32 1481 32 8221
Fragments arranged by size:
8221 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2146 3448 5184 2146
Size: 1302 1736 6728
Fragments arranged by size:
6728 1736 1302
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9766
PsiI TTA'TAA
Cuts at: 7336 7336
Size: 9766
PspOMI G'GGCC_C
Cuts at: 3862 5501 5980 3862
Size: 1639 479 7648
Fragments arranged by size:
7648 1639 479
PspXI vC'TCGA_Gb
Cuts at: 5390 5390
Size: 9766
PstI C_TGCA'G
Cuts at: 830 2885 3588 3653 4906 4966 5026 5153 5417
Size: 2055 703 65 1253 60 60 127 264
Cuts at: 5417 5488 5696 5968 7118 830
Size: 71 208 272 1150 3478
Fragments arranged by size:
3478 2055 1253 1150 703 272 264 208
127 71 65 60 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9766
PvuII CAG'CTG
Cuts at: 2357 3502 3901 4080 5838 6313 6571 7012 2357
Size: 1145 399 179 1758 475 258 441 5111
Fragments arranged by size:
5111 1758 1145 475 441 399 258 179
SalI G'TCGA_C
Cuts at: 1936 7108 1936
Size: 5172 4594
SanDI GG'GwC_CC
Cuts at: 2146 5184 2146
Size: 3038 6728
SapI GCTCTTCn'nnn_
Cuts at: 3108 4857 3108
Size: 1749 8017
SbfI CC_TGCA'GG
Cuts at: 4906 4966 5026 7118 4906
Size: 60 60 2092 7554
Fragments arranged by size:
7554 2092 60 60
ScaI AGT'ACT
Cuts at: 6632 8014 9726 6632
Size: 1382 1712 6672
Fragments arranged by size:
6672 1712 1382
SfcI C'TryA_G
Cuts at: 826 1045 2680 2881 3584 3649 4902 4962 5022
Size: 219 1635 201 703 65 1253 60 60
Cuts at: 5022 5149 5413 5484 5516 5692 5794 5964 7114
Size: 127 264 71 32 176 102 170 1150
Cuts at: 7114 8249 8930 9121 826
Size: 1135 681 191 1471
Fragments arranged by size:
1635 1471 1253 1150 1135 703 681 264
219 201 191 176 170 127 102 71
65 60 60 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9766
SgrAI Cr'CCGG_yG
Cuts at: 1700 5571 1700
Size: 3871 5895
SmaI CCC'GGG
Cuts at: 1872 2144 6173 7093 1872
Size: 272 4029 920 4545
Fragments arranged by size:
4545 4029 920 272
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2621 2819 4139 4415 5390 5452
Size: 197 926 678 198 1320 276 975 62
Cuts at: 5452 5579 5906 6065 6488 7870 8771 9033 820
Size: 127 327 159 423 1382 901 262 1553
Fragments arranged by size:
1553 1382 1320 975 926 901 678 423
327 276 262 198 197 159 127 62
SnaBI TAC'GTA
Cuts at: 493 7557 493
Size: 7064 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9766
SphI G_CATG'C
Cuts at: 1132 2728 3191 5563 5918 7124 1132
Size: 1596 463 2372 355 1206 3774
Fragments arranged by size:
3774 2372 1596 1206 463 355
SspI AAT'ATT
Cuts at: 5 52 7690 5
Size: 47 7638 2081
Fragments arranged by size:
7638 2081 47
StuI AGG'CCT
Cuts at: 1517 2909 3815 4754 6089 6722 1517
Size: 1392 906 939 1335 633 4561
Fragments arranged by size:
4561 1392 1335 939 906 633
StyI C'CwwG_G
Cuts at: 513 1065 2431 2935 3000 3366 3790 4027 4582
Size: 552 1366 504 65 366 424 237 555
Cuts at: 4582 5845 7205 513
Size: 1263 1360 3074
Fragments arranged by size:
3074 1366 1360 1263 555 552 504 424
366 237 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2411 2636 4031 4244 4566 4667 4732
Size: 1255 831 225 1395 213 322 101 65
Cuts at: 4732 4993 5053 6072 6764 6860 7793 7810 7963
Size: 261 60 1019 692 96 933 17 153
Cuts at: 7963 8148 8593 325
Size: 185 445 1498
Fragments arranged by size:
1498 1395 1255 1019 933 831 692 445
322 261 225 213 185 153 101 96
65 60 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5252 6630 8012
Size: 274 80 33 51 1443 3275 1378 1382
Cuts at: 8012 9724 96
Size: 1712 138
Fragments arranged by size:
3275 1712 1443 1382 1378 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2048 2080 3362 3394 3584 3616 3756 3788 4294
Size: 32 1282 32 190 32 140 32 506
Cuts at: 4294 4326 5075 5107 9726 9758 2048
Size: 32 749 32 4619 32 2056
Fragments arranged by size:
4619 2056 1282 749 506 190 140 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4952 5012 1316
Size: 3636 60 6070
Fragments arranged by size:
6070 3636 60
XbaI T'CTAG_A
Cuts at: 7102 7102
Size: 9766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3611 5519 3611
Size: 1908 7858
XhoI C'TCGA_G
Cuts at: 1943 5390 1943
Size: 3447 6319
XmaI C'CCGG_G
Cuts at: 1870 2142 6171 7091 1870
Size: 272 4029 920 4545
Fragments arranged by size:
4545 4029 920 272
XmnI GAAnn'nnTTC
Cuts at: 2600 7895 2600
Size: 5295 4471
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7574 276
Size: 53 83 186 936 6040 2468
Fragments arranged by size:
6040 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BstZ17I EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI
RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI