Restriction map for
pFN21AA0233
(Circular) (Six-base) MAPSORT of: pFN21AA0233.seq Check: 3060 from: 1 to: 12268
pFN21AA0233 12268 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4312 4402 4809 5335 4312
Size: 90 407 526 11245
Fragments arranged by size:
11245 526 407 90
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4398 7884 10078 278
Size: 53 83 186 936 2862 3486 2194 2468
Fragments arranged by size:
3486 2862 2468 2194 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2103 2670 3801 3813 3855 3936 7615 7914
Size: 166 567 1131 12 42 81 3679 299
Cuts at: 7914 9611 1937
Size: 1697 4594
Fragments arranged by size:
4594 3679 1697 1131 567 299 166 81
42 12
Acc65I G'GTAC_C
Cuts at: 8002 9589 8002
Size: 1587 10681
AclI AA'CG_TT
Cuts at: 1563 10395 10768 1563
Size: 8832 373 3063
Fragments arranged by size:
8832 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2068 2360 2722 3641 4350 4505 5498 5749
Size: 436 292 362 919 709 155 993 251
Cuts at: 5749 6324 9364 10331 11715 11983 1632
Size: 575 3040 967 1384 268 1917
Fragments arranged by size:
3040 1917 1384 993 967 919 709 575
436 362 292 268 251 155
AfeI AGC'GCT
Cuts at: 6644 7187 6644
Size: 543 11725
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12071
AflIII A'CryG_T
Cuts at: 1203 3450 3816 4143 4405 9336 11167 12144 1203
Size: 2247 366 327 262 4931 1831 977 1327
Fragments arranged by size:
4931 2247 1831 1327 977 366 327 262
AgeI A'CCGG_T
Cuts at: 11099 11099
Size: 12268
AhdI GACnn_n'nnGTC
Cuts at: 7978 10997 7978
Size: 3019 9249
AleI CACnn'nnGTG
Cuts at: 1166 4322 1166
Size: 3156 9112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4076 4706 5627 8099 9272 9383 1184
Size: 2892 630 921 2472 1173 111 4069
Fragments arranged by size:
4069 2892 2472 1173 921 630 111
AlwNI CAG_nnn'CTG
Cuts at: 2275 2295 2748 2824 3694 3706 4659 4717 4927
Size: 20 453 76 870 12 953 58 210
Cuts at: 4927 4939 5164 5874 6544 6925 7405 8758 10011
Size: 12 225 710 670 381 480 1353 1253
Cuts at: 10011 11583 2275
Size: 1572 2960
Fragments arranged by size:
2960 1572 1353 1253 953 870 710 670
480 453 381 225 210 76 58 20
12 12
ApaI G_GGCC'C
Cuts at: 3497 4734 5284 5635 6143 6171 6482 7646 9102
Size: 1237 550 351 508 28 311 1164 1456
Cuts at: 9102 3497
Size: 6663
Fragments arranged by size:
6663 1456 1237 1164 550 508 351 311
28
ApaLI G'TGCA_C
Cuts at: 3158 6683 10325 11481 3158
Size: 3525 3642 1156 3945
Fragments arranged by size:
3945 3642 3525 1156
ApoI r'AATT_y
Cuts at: 1493 4982 9577 9803 9955 12064 1493
Size: 3489 4595 226 152 2109 1697
Fragments arranged by size:
4595 3489 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 10822 160
Size: 872 9790 1606
Fragments arranged by size:
9790 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12268
AvaI C'yCGr_G
Cuts at: 1870 1943 2730 3054 3221 6310 6482 6887 7171
Size: 73 787 324 167 3089 172 405 284
Cuts at: 7171 8006 9029 9474 9593 1870
Size: 835 1023 445 119 4545
Fragments arranged by size:
4545 3089 1023 835 787 445 405 324
284 172 167 119 73
BamHI G'GATC_C
Cuts at: 9598 9965 9598
Size: 367 11901
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2424 2771 3118 3554 4060
Size: 325 217 627 637 347 347 436 506
Cuts at: 4060 4324 4845 4913 5091 6606 6614 6905 6960
Size: 264 521 68 178 1515 8 291 55
Cuts at: 6960 7501 8002 8338 9035 9589 11044 618
Size: 541 501 336 697 554 1455 1842
Fragments arranged by size:
1842 1515 1455 697 637 627 554 541
521 506 501 436 347 347 336 325
291 264 217 178 68 55 8
BanII G_rGCy'C
Cuts at: 1391 2453 3182 3222 3497 3678 4044 4180 4671
Size: 1062 729 40 275 181 366 136 491
Cuts at: 4671 4734 5284 5430 5635 6143 6171 6188 6482
Size: 63 550 146 205 508 28 17 294
Cuts at: 6482 6666 7587 7646 8880 9102 9587 1391
Size: 184 921 59 1234 222 485 4072
Fragments arranged by size:
4072 1234 1062 921 729 550 508 491
485 366 294 275 222 205 184 181
146 136 63 59 40 28 17
BbeI G_GCGC'C
Cuts at: 2428 2775 4849 6964 2428
Size: 347 2074 2115 7732
Fragments arranged by size:
7732 2115 2074 347
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5482 5710 5899 6312 7138 7201 7343
Size: 971 3583 228 189 413 826 63 142
Cuts at: 7343 7527 928
Size: 184 5669
Fragments arranged by size:
5669 3583 971 826 413 228 189 184
142 63
BbvCI CC'TCA_GC
Cuts at: 2201 6380 7677 2201
Size: 4179 1297 6792
Fragments arranged by size:
6792 4179 1297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3751 3785 7868 7902 8206 8240 10459
Size: 34 1814 34 4083 34 304 34 2219
Cuts at: 10459 10493 1903
Size: 34 3678
Fragments arranged by size:
4083 3678 2219 1814 304 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6083 9173 10160 11370 6083
Size: 3090 987 1210 6981
Fragments arranged by size:
6981 3090 1210 987
BclI T'GATC_A
Cuts at: 1548 9543 1548
Size: 7995 4273
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3018 3202 6202
Size: 107 122 71 631 605 1346 184 3000
Cuts at: 6202 7831 8494 10879 136
Size: 1629 663 2385 1525
Fragments arranged by size:
3000 2385 1629 1525 1346 663 631 605
184 122 107 71
BglII A'GATC_T
Cuts at: 2238 4995 9387 12263 2238
Size: 2757 4392 2876 2243
Fragments arranged by size:
4392 2876 2757 2243
BlpI GC'TnA_GC
Cuts at: 3216 4359 5358 6527 9685 3216
Size: 1143 999 1169 3158 5799
Fragments arranged by size:
5799 3158 1169 1143 999
Bme1580I G_kGCm'C
Cuts at: 1790 2259 3123 3162 3497 4065 4734 5284 5442
Size: 469 864 39 335 568 669 550 158
Cuts at: 5442 5635 6143 6171 6482 6687 7646 7817 8863
Size: 193 508 28 311 205 959 171 1046
Cuts at: 8863 9038 9091 9102 10329 11485 1790
Size: 175 53 11 1227 1156 2573
Fragments arranged by size:
2573 1227 1156 1046 959 864 669 568
550 508 469 335 311 205 193 175
171 158 53 39 28 11
BmgBI CAC'GTC
Cuts at: 1318 5932 6628 7980 1318
Size: 4614 696 1352 5606
Fragments arranged by size:
5606 4614 1352 696
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 8383 9999 10957
Size: 416 53 83 399 24 6966 1616 958
Cuts at: 10957 442
Size: 1753
Fragments arranged by size:
6966 1753 1616 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3071 5966 1056
Size: 2015 2895 7358
Fragments arranged by size:
7358 2895 2015
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2323 4063 4291 7453 8839 9055 2323
Size: 1740 228 3162 1386 216 5536
Fragments arranged by size:
5536 3162 1740 1386 228 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2647 2878 3083 3542 3621 4033 4040
Size: 980 428 231 205 459 79 412 7
Cuts at: 4040 4261 4391 4454 5012 5236 5429 5930 5953
Size: 221 130 63 558 224 193 501 23
Cuts at: 5953 6179 7001 7352 7773 9503 9509 10928 1239
Size: 226 822 351 421 1730 6 1419 2579
Fragments arranged by size:
2579 1730 1419 980 822 558 501 459
428 421 412 351 231 226 224 221
205 193 130 79 63 23 7 6
Bpu10I CC'TnA_GC
Cuts at: 2201 3474 5912 6380 6836 7586 7677 2201
Size: 1273 2438 468 456 750 91 6792
Fragments arranged by size:
6792 2438 1273 750 468 456 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3866 4610 4937 5987 8630 9422 10393 11258
Size: 1017 744 327 1050 2643 792 971 865
Cuts at: 11258 11556 2849
Size: 298 3561
Fragments arranged by size:
3561 2643 1050 1017 971 865 792 744
327 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2294 2401 2867 5814 9530 10931 882
Size: 617 795 107 466 2947 3716 1401 2219
Fragments arranged by size:
3716 2947 2219 1401 795 617 466 107
BsaAI yAC'GTr
Cuts at: 493 1204 2017 3115 3451 4144 7276 9337 10059
Size: 711 813 1098 336 693 3132 2061 722
Cuts at: 10059 493
Size: 2702
Fragments arranged by size:
3132 2702 2061 1098 813 722 711 693
336
BsaBI GATnn'nnATC
Cuts at: 12262 12262
Size: 12268
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2425 2772 2955 3230
Size: 53 83 186 936 892 347 183 275
Cuts at: 3230 4395 4846 5391 6961 7881 10075 10457 11092
Size: 1165 451 545 1570 920 2194 382 635
Cuts at: 11092 275
Size: 1451
Fragments arranged by size:
2194 1570 1451 1165 936 920 892 635
545 451 382 347 275 186 183 83
53
BsaWI w'CCGG_w
Cuts at: 9532 10701 11099 11373 11520 11931 9532
Size: 1169 398 274 147 411 9869
Fragments arranged by size:
9869 1169 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2999 3029 4130 4160 6648
Size: 30 165 30 1596 30 1101 30 2488
Cuts at: 6648 6678 7746 7776 8937 8967 9404 9434 9482
Size: 30 1068 30 1161 30 437 30 48
Cuts at: 9482 9485 9512 9515 1178
Size: 3 27 3 3931
Fragments arranged by size:
3931 2488 1596 1161 1101 1068 437 165
48 30 30 30 30 30 30 30
30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2389 4235 4268 4271 4274 4277 4280 4283
Size: 1203 1846 33 3 3 3 3 3
Cuts at: 4283 5384 5861 6047 6178 6302 6305 6500 6641
Size: 1101 477 186 131 124 3 195 141
Cuts at: 6641 6809 6884 6998 7445 7484 7487 7502 8741
Size: 168 75 114 447 39 3 15 1239
Cuts at: 8741 9152 9425 9506 9509 1186
Size: 411 273 81 3 3945
Fragments arranged by size:
3945 1846 1239 1203 1101 477 447 411
273 195 186 168 141 131 124 114
81 75 39 33 15 3 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2194 2282 2295 2600 2857 3514 4003 4173 4291
Size: 88 13 305 257 657 489 170 118
Cuts at: 4291 5334 5563 5748 6048 6452 6553 6784 7178
Size: 1043 229 185 300 404 101 231 394
Cuts at: 7178 7398 7645 8086 8193 8650 8685 8751 8852
Size: 220 247 441 107 457 35 66 101
Cuts at: 8852 11471 11967 2194
Size: 2619 496 2495
Fragments arranged by size:
2619 2495 1043 657 496 489 457 441
404 394 305 300 257 247 231 229
220 185 170 118 107 101 101 88
66 35 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2680 4082 4430 4645 5039 5912 6536
Size: 573 944 1402 348 215 394 873 624
Cuts at: 6536 7388 7838 8988 1163
Size: 852 450 1150 4443
Fragments arranged by size:
4443 1402 1150 944 873 852 624 573
450 394 348 215
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3790 5404 7773 7826 8895 9718
Size: 275 186 1789 1614 2369 53 1069 823
Cuts at: 9718 10479 11507 1540
Size: 761 1028 2301
Fragments arranged by size:
2369 2301 1789 1614 1069 1028 823 761
275 186 53
BsiHKAI G_wGCw'C
Cuts at: 2022 3162 3182 3404 3528 4044 4143 4857 6533
Size: 1140 20 222 124 516 99 714 1676
Cuts at: 6533 6666 6687 6842 7143 7587 8880 10329 10414
Size: 133 21 155 301 444 1293 1449 85
Cuts at: 10414 11485 2022
Size: 1071 2805
Fragments arranged by size:
2805 1676 1449 1293 1140 1071 714 516
444 301 222 155 133 124 99 85
21 20
BsmI GAATG_Cn'
Cuts at: 9779 9872 9779
Size: 93 12175
BsmBI CGTCTCn'nnnn_
Cuts at: 3237 3237
Size: 12268
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2259 2453 3123 3162 3182 3222
Size: 399 232 237 194 670 39 20 40
Cuts at: 3222 3404 3497 3528 3678 4044 4065 4143 4180
Size: 182 93 31 150 366 21 78 37
Cuts at: 4180 4671 4734 4857 5284 5430 5442 5635 6143
Size: 491 63 123 427 146 12 193 508
Cuts at: 6143 6171 6188 6482 6533 6666 6687 6842 7143
Size: 28 17 294 51 133 21 155 301
Cuts at: 7143 7587 7646 7817 8863 8880 9038 9091 9102
Size: 444 59 171 1046 17 158 53 11
Cuts at: 9102 9587 10329 10414 11485 1391
Size: 485 742 85 1071 2174
Fragments arranged by size:
2174 1071 1046 742 670 508 491 485
444 427 399 366 301 294 237 232
194 193 182 171 158 155 150 146
133 123 93 85 78 63 59 53
51 40 39 37 31 28 21 21
20 17 17 12 11
BspEI T'CCGG_A
Cuts at: 11931 11931
Size: 12268
BspHI T'CATG_A
Cuts at: 10157 11887 10157
Size: 1730 10538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2115 2682 3474 4312 4402 4515 4598 4809
Size: 1271 567 792 838 90 113 83 211
Cuts at: 4809 5172 5271 5335 5448 5580 5598 5934 8079
Size: 363 99 64 113 132 18 336 2145
Cuts at: 8079 9261 9623 844
Size: 1182 362 3489
Fragments arranged by size:
3489 2145 1271 1182 838 792 567 363
362 336 211 132 113 113 99 90
83 64 18
BsrBI CCG'CTC
Cuts at: 1394 1823 2561 3238 4363 7395 7464 7661 7749
Size: 429 738 677 1125 3032 69 197 88
Cuts at: 7749 9528 10155 1394
Size: 1779 627 3507
Fragments arranged by size:
3507 3032 1779 1125 738 677 627 429
197 88 69
BsrDI GCAATG_nn'
Cuts at: 65 1248 2189 3288 5103 10763 10937 65
Size: 1183 941 1099 1815 5660 174 1396
Fragments arranged by size:
5660 1815 1396 1183 1099 941 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2774 2837 2921 3170 3785 4382 4842
Size: 110 964 63 84 249 615 597 460
Cuts at: 4842 4863 5738 6842 7118 9197 10912 11099 1700
Size: 21 875 1104 276 2079 1715 187 2869
Fragments arranged by size:
2869 2079 1715 1104 964 875 615 597
460 276 249 187 110 84 63 21
BsrGI T'GTAC_A
Cuts at: 96 4464 5664 6708 96
Size: 4368 1200 1044 5656
Fragments arranged by size:
5656 4368 1200 1044
BssHII G'CGCG_C
Cuts at: 1925 2055 3282 1925
Size: 130 1227 10911
Fragments arranged by size:
10911 1227 130
BssSI C'ACGA_G
Cuts at: 3161 3794 3899 5639 9555 10328 11340 3161
Size: 633 105 1740 3916 773 1012 4089
Fragments arranged by size:
4089 3916 1740 1012 773 633 105
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2254 2748 3118 4810 6329 6523 794
Size: 1460 494 370 1692 1519 194 6539
Fragments arranged by size:
6539 1692 1519 1460 494 370 194
BstBI TT'CG_AA
Cuts at: 11072 11072
Size: 12268
BstEII G'GTnAC_C
Cuts at: 1184 3655 5047 6358 1184
Size: 2471 1392 1311 7094
Fragments arranged by size:
7094 2471 1392 1311
BstXI CCAn_nnnn'nTGG
Cuts at: 2365 3490 4057 6784 9349 2365
Size: 1125 567 2727 2565 5284
Fragments arranged by size:
5284 2727 2565 1125 567
BstYI r'GATC_y
Cuts at: 1972 2238 3744 4936 4995 8244 9387 9598 9965
Size: 266 1506 1192 59 3249 1143 211 367
Cuts at: 9965 10350 10367 11819 11905 11917 12263 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
3249 1977 1506 1452 1192 1143 385 367
346 266 211 86 59 17 12
Bsu36I CC'TnA_GG
Cuts at: 8790 8790
Size: 12268
BtgI C'CryG_G
Cuts at: 513 1065 2004 2459 2754 2979 3459 3660 4560
Size: 552 939 455 295 225 480 201 900
Cuts at: 4560 4698 4734 6197 6610 7554 7722 8046 8601
Size: 138 36 1463 413 944 168 324 555
Cuts at: 8601 9261 513
Size: 660 3520
Fragments arranged by size:
3520 1463 944 939 900 660 555 552
480 455 413 324 295 225 201 168
138 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2626 3913 8539 487
Size: 683 56 1400 1287 4626 4216
Fragments arranged by size:
4626 4216 1400 1287 683 56
BtsI GCAGTG_nn'
Cuts at: 2621 4200 4208 5345 5452 6212 6865 6871 8370
Size: 1579 8 1137 107 760 653 6 1499
Cuts at: 8370 9000 9784 10578 10598 2621
Size: 630 784 794 20 4291
Fragments arranged by size:
4291 1579 1499 1137 794 784 760 653
630 107 20 8 6
ClaI AT'CG_AT
Cuts at: 1554 8628 1554
Size: 7074 5194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12233
DraI TTT'AAA
Cuts at: 9572 9572
Size: 12268
DraIII CAC_nnn'GTG
Cuts at: 2703 3118 4584 6329 7279 2703
Size: 415 1466 1745 950 7692
Fragments arranged by size:
7692 1745 1466 950 415
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 11275 809
Size: 507 9959 1802
Fragments arranged by size:
9959 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2556 2613 2776
Size: 54 1425 50 275 18 726 57 163
Cuts at: 2776 3019 3172 3390 3787 4299 5010 5073 5484
Size: 243 153 218 397 512 711 63 411
Cuts at: 5484 6936 7053 7200 7509 7770 7823 7965 8121
Size: 1452 117 147 309 261 53 142 156
Cuts at: 8121 8760 8892 9018 9715 10604 12233 8
Size: 639 132 126 697 889 1629 43
Fragments arranged by size:
1629 1452 1425 889 726 711 697 639
512 411 397 309 275 261 243 218
163 156 153 147 142 132 126 117
63 57 54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3787 7770 7823 8892 9715 1537
Size: 275 1975 3983 53 1069 823 4090
Fragments arranged by size:
4090 3983 1975 1069 823 275 53
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 2216 2687 3830 3851 4049 5138 5603
Size: 764 99 471 1143 21 198 1089 465
Cuts at: 5603 5768 6279 7689 7701 7730 7753 8318 8324
Size: 165 511 1410 12 29 23 565 6
Cuts at: 8324 8552 8585 9074 10198 1353
Size: 228 33 489 1124 3423
Fragments arranged by size:
3423 1410 1143 1124 1089 764 565 511
489 471 465 228 198 165 99 33
29 23 21 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3886 7105 8224 10855 11229 11375
Size: 1064 138 2297 3219 1119 2631 374 146
Cuts at: 11375 387
Size: 1280
Fragments arranged by size:
3219 2631 2297 1280 1119 1064 374 146
138
EcoICRI GAG'CTC
Cuts at: 3180 4042 6664 7585 8878 3180
Size: 862 2622 921 1293 6570
Fragments arranged by size:
6570 2622 1293 921 862
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2219 2360 2647 2722 2878 3083
Size: 393 436 151 141 287 75 156 205
Cuts at: 3083 3542 3621 3641 4033 4040 4261 4350 4391
Size: 459 79 20 392 7 221 89 41
Cuts at: 4391 4454 4505 5012 5236 5429 5498 5749 5930
Size: 63 51 507 224 193 69 251 181
Cuts at: 5930 5953 6179 6324 7001 7352 7773 9364 9503
Size: 23 226 145 677 351 421 1591 139
Cuts at: 9503 9509 10331 10928 11715 11983 1239
Size: 6 822 597 787 268 1524
Fragments arranged by size:
1591 1524 822 787 677 597 507 459
436 421 393 392 351 287 268 251
226 224 221 205 193 181 156 151
145 141 139 89 79 75 69 63
51 41 23 20 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3848 4487 6656 7808 8321 8939 9029 3848
Size: 639 2169 1152 513 618 90 7087
Fragments arranged by size:
7087 2169 1152 639 618 513 90
EcoO109I rG'GnC_Cy
Cuts at: 2279 2608 2879 3494 4009 4113 4203 4348 4730
Size: 329 271 615 515 104 90 145 382
Cuts at: 4730 5281 5460 6140 6148 6167 6478 6479 6740
Size: 551 179 680 8 19 311 1 261
Cuts at: 6740 6751 7563 7590 7642 7717 8932 2279
Size: 11 812 27 52 75 1215 5615
Fragments arranged by size:
5615 1215 812 680 615 551 515 382
329 311 271 261 179 145 104 90
75 52 27 19 11 8 1
EcoRI G'AATT_C
Cuts at: 9577 9955 9577
Size: 378 11890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7324 8116 9532 7324
Size: 792 1416 10060
Fragments arranged by size:
10060 1416 792
FspI TGC'GCA
Cuts at: 2993 3374 6968 8075 10774 2993
Size: 381 3594 1107 2699 4487
Fragments arranged by size:
4487 3594 2699 1107 381
HaeII r_GCGC'y
Cuts at: 1678 2428 2775 4849 5144 5215 5902 6566 6646
Size: 750 347 2074 295 71 687 664 80
Cuts at: 6646 6964 7022 7189 7291 7837 8114 8685 8834
Size: 318 58 167 102 546 277 571 149
Cuts at: 8834 11415 1678
Size: 2581 2531
Fragments arranged by size:
2581 2531 2074 750 687 664 571 546
347 318 295 277 167 149 102 80
71 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2142 2174 2440 2472 4130
Size: 32 135 32 570 32 266 32 1658
Cuts at: 4130 4162 4385 4417 6023 6055 7427 7459 7964
Size: 32 223 32 1606 32 1372 32 505
Cuts at: 7964 7996 8717 8749 10909 10941 10983 11015 1373
Size: 32 721 32 2160 32 42 32 2626
Fragments arranged by size:
2626 2160 1658 1606 1372 721 570 505
266 223 135 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4636 5395 7915 8056 8617 9612
Size: 988 281 2698 759 2520 141 561 995
Cuts at: 9612 9858 669
Size: 246 3079
Fragments arranged by size:
3079 2698 2520 995 988 759 561 281
246 141
HindIII A'AGCT_T
Cuts at: 748 748
Size: 12268
HpaI GTT'AAC
Cuts at: 9858 9858
Size: 12268
KasI G'GCGC_C
Cuts at: 2424 2771 4845 6960 2424
Size: 347 2074 2115 7732
Fragments arranged by size:
7732 2115 2074 347
KpnI G_GTAC'C
Cuts at: 8006 9593 8006
Size: 1587 10681
MfeI C'AATT_G
Cuts at: 9867 9867
Size: 12268
MluI A'CGCG_T
Cuts at: 4405 12144 4405
Size: 7739 4529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6297 7528 11382 11566 1305
Size: 4992 1231 3854 184 2007
Fragments arranged by size:
4992 3854 2007 1231 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3392 4301 5075 8123 8762
Size: 54 1425 343 1560 909 774 3048 639
Cuts at: 8762 9020 10
Size: 258 3258
Fragments arranged by size:
3258 3048 1560 1425 909 774 639 343
258 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 3293 4058 4133 4322 4601 7532
Size: 648 1224 903 765 75 189 279 2931
Cuts at: 7532 10226 10585 10744 518
Size: 2694 359 159 2042
Fragments arranged by size:
2931 2694 2042 1224 903 765 648 359
279 189 159 75
MspA1I CmG'CkG
Cuts at: 1606 2088 2286 2295 2756 3324 3514 3641 4003
Size: 482 198 9 461 568 190 127 362
Cuts at: 4003 4096 4876 4885 4911 4927 5357 5416 6052
Size: 93 780 9 26 16 430 59 636
Cuts at: 6052 6340 6494 6706 7402 8250 8409 8575 8668
Size: 288 154 212 696 848 159 166 93
Cuts at: 8668 8758 9016 9124 9169 9684 10361 11509 11754
Size: 90 258 108 45 515 677 1148 245
Cuts at: 11754 1606
Size: 2120
Fragments arranged by size:
2120 1148 848 780 696 677 636 568
515 482 461 430 362 288 258 245
212 198 190 166 159 154 127 108
93 93 90 59 45 26 16 9
9
NaeI GCC'GGC
Cuts at: 2776 2839 3787 4384 4865 7120 2776
Size: 63 948 597 481 2255 7924
Fragments arranged by size:
7924 2255 948 597 481 63
NarI GG'CG_CC
Cuts at: 2425 2772 4846 6961 2425
Size: 347 2074 2115 7732
Fragments arranged by size:
7732 2115 2074 347
NcoI C'CATG_G
Cuts at: 513 1065 2004 3459 4560 8046 9261 513
Size: 552 939 1455 1101 3486 1215 3520
Fragments arranged by size:
3520 3486 1455 1215 1101 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 12268
NgoMIV G'CCGG_C
Cuts at: 2774 2837 3785 4382 4863 7118 2774
Size: 63 948 597 481 2255 7924
Fragments arranged by size:
7924 2255 948 597 481 63
NheI G'CTAG_C
Cuts at: 1052 3067 5962 1052
Size: 2015 2895 7358
Fragments arranged by size:
7358 2895 2015
NotI GC'GGCC_GC
Cuts at: 9715 9715
Size: 12268
NruI TCG'CGA
Cuts at: 2311 2311
Size: 12268
NspI r_CATG'y
Cuts at: 1132 1229 3766 3820 5476 5698 8980 9626 11171
Size: 97 2537 54 1656 222 3282 646 1545
Cuts at: 11171 1132
Size: 2229
Fragments arranged by size:
3282 2537 2229 1656 1545 646 222 97
54
PasI CC'CwG_GG
Cuts at: 1348 3490 6136 7810 1348
Size: 2142 2646 1674 5806
Fragments arranged by size:
5806 2646 2142 1674
PciI A'CATG_T
Cuts at: 3816 11167 3816
Size: 7351 4917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2275 2908 3115 3247 3388 3741 4584 4927
Size: 1167 633 207 132 141 353 843 343
Cuts at: 4927 6463 7051 8185 8565 8758 9052 1108
Size: 1536 588 1134 380 193 294 4324
Fragments arranged by size:
4324 1536 1167 1134 843 633 588 380
353 343 294 207 193 141 132
PfoI T'CCnGG_A
Cuts at: 3951 6951 3951
Size: 3000 9268
PmeI GTTT'AAAC
Cuts at: 9572 9572
Size: 12268
PmlI CAC'GTG
Cuts at: 2017 3115 4144 7276 2017
Size: 1098 1029 3132 7009
Fragments arranged by size:
7009 3132 1098 1029
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8156 8188 8654 8686 10337 10369 11850 11882 8156
Size: 32 466 32 1651 32 1481 32 8542
Fragments arranged by size:
8542 1651 1481 466 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2279 2608 2879 4009 4203 5460 6148 7563 8932
Size: 329 271 1130 194 1257 688 1415 1369
Cuts at: 8932 2279
Size: 5615
Fragments arranged by size:
5615 1415 1369 1257 1130 688 329 271
194
PshAI GACnn'nnGTC
Cuts at: 1613 3653 4592 6117 1613
Size: 2040 939 1525 7764
Fragments arranged by size:
7764 2040 1525 939
PsiI TTA'TAA
Cuts at: 9838 9838
Size: 12268
PspOMI G'GGCC_C
Cuts at: 3493 4730 5280 5631 6139 6167 6478 7642 9098
Size: 1237 550 351 508 28 311 1164 1456
Cuts at: 9098 3493
Size: 6663
Fragments arranged by size:
6663 1456 1237 1164 550 508 351 311
28
PspXI vC'TCGA_Gb
Cuts at: 9029 9029
Size: 12268
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7289 7321 7289
Size: 32 12236
PstI C_TGCA'G
Cuts at: 830 2291 2517 4095 5459 5619 5898 6594 6672
Size: 1461 226 1578 1364 160 279 696 78
Cuts at: 6672 7788 9129 9231 9620 830
Size: 1116 1341 102 389 3478
Fragments arranged by size:
3478 1578 1461 1364 1341 1116 696 389
279 226 160 102 78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12268
PvuII CAG'CTG
Cuts at: 2286 3324 3514 3641 4003 4096 4876 4927 5357
Size: 1038 190 127 362 93 780 51 430
Cuts at: 5357 6052 6340 6706 7402 8409 9016 9124 9169
Size: 695 288 366 696 1007 607 108 45
Cuts at: 9169 2286
Size: 5385
Fragments arranged by size:
5385 1038 1007 780 696 695 607 430
366 362 288 190 127 108 93 51
45
RsrII CG'GwC_CG
Cuts at: 9240 9240
Size: 12268
SacI G_AGCT'C
Cuts at: 3182 4044 6666 7587 8880 3182
Size: 862 2622 921 1293 6570
Fragments arranged by size:
6570 2622 1293 921 862
SacII CC_GC'GG
Cuts at: 2757 2757
Size: 12268
SalI G'TCGA_C
Cuts at: 1936 7913 9610 1936
Size: 5977 1697 4594
Fragments arranged by size:
5977 4594 1697
SanDI GG'GwC_CC
Cuts at: 2279 2879 4203 7563 2279
Size: 600 1324 3360 6984
Fragments arranged by size:
6984 3360 1324 600
SapI GCTCTTCn'nnn_
Cuts at: 2117 2216 2687 4049 5603 5768 8585 2117
Size: 99 471 1362 1554 165 2817 5800
Fragments arranged by size:
5800 2817 1554 1362 471 165 99
SbfI CC_TGCA'GG
Cuts at: 6672 9620 6672
Size: 2948 9320
ScaI AGT'ACT
Cuts at: 3740 4958 10516 12228 3740
Size: 1218 5558 1712 3780
Fragments arranged by size:
5558 3780 1712 1218
SexAI A'CCwGG_T
Cuts at: 2699 7362 7998 2699
Size: 4663 636 6969
Fragments arranged by size:
6969 4663 636
SfcI C'TryA_G
Cuts at: 826 1045 2287 2513 3859 4091 5455 5615 5894
Size: 219 1242 226 1346 232 1364 160 279
Cuts at: 5894 6590 6668 7784 9125 9227 9616 10751 11432
Size: 696 78 1116 1341 102 389 1135 681
Cuts at: 11432 11623 826
Size: 191 1471
Fragments arranged by size:
1471 1364 1346 1341 1242 1135 1116 696
681 389 279 232 226 219 191 160
102 78
SfiI GGCCn_nnn'nGGCC
Cuts at: 3202 3202
Size: 12268
SfoI GGC'GCC
Cuts at: 2426 2773 4847 6962 2426
Size: 347 2074 2115 7732
Fragments arranged by size:
7732 2115 2074 347
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12268
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12268
SmaI CCC'GGG
Cuts at: 1872 3223 6889 9595 1872
Size: 1351 3666 2706 4545
Fragments arranged by size:
4545 3666 2706 1351
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2864 3881 4625 4952 6002 8645
Size: 197 926 921 1017 744 327 1050 2643
Cuts at: 8645 9029 9437 10372 11273 11535 820
Size: 384 408 935 901 262 1553
Fragments arranged by size:
2643 1553 1050 1017 935 926 921 901
744 408 384 327 262 197
SnaBI TAC'GTA
Cuts at: 493 10059 493
Size: 9566 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12268
SphI G_CATG'C
Cuts at: 1132 9626 1132
Size: 8494 3774
SspI AAT'ATT
Cuts at: 5 52 10192 5
Size: 47 10140 2081
Fragments arranged by size:
10140 2081 47
StuI AGG'CCT
Cuts at: 1517 3887 8720 1517
Size: 2370 4833 5065
Fragments arranged by size:
5065 4833 2370
StyI C'CwwG_G
Cuts at: 513 1065 2004 2161 2811 3459 3550 4560 5802
Size: 552 939 157 650 648 91 1010 1242
Cuts at: 5802 6144 7446 8046 9261 9707 513
Size: 342 1302 600 1215 446 3074
Fragments arranged by size:
3074 1302 1242 1215 1010 939 650 648
600 552 446 342 157 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4465 6346 8365 8519 9257 10295 10312
Size: 1255 2885 1881 2019 154 738 1038 17
Cuts at: 10312 10465 10650 11095 325
Size: 153 185 445 1498
Fragments arranged by size:
2885 2019 1881 1498 1255 1038 738 445
185 154 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2034 3738 4464
Size: 274 80 33 51 1443 57 1704 726
Cuts at: 4464 4956 5664 6708 8295 8781 9078 10514 12226
Size: 492 708 1044 1587 486 297 1436 1712
Cuts at: 12226 96
Size: 138
Fragments arranged by size:
1712 1704 1587 1443 1436 1044 726 708
492 486 297 274 138 80 57 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5444 5476 6648 6680 8363 8395 8937 8969 9404
Size: 32 1172 32 1683 32 542 32 435
Cuts at: 9404 9436 12228 12260 5444
Size: 32 2792 32 5452
Fragments arranged by size:
5452 2792 1683 1172 542 435 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5870 8798 9281 1316
Size: 4554 2928 483 4303
Fragments arranged by size:
4554 4303 2928 483
XbaI T'CTAG_A
Cuts at: 9604 9604
Size: 12268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2365 4348 5809 6204 6460 6857 2365
Size: 1983 1461 395 256 397 7776
Fragments arranged by size:
7776 1983 1461 397 395 256
XhoI C'TCGA_G
Cuts at: 1943 9029 1943
Size: 7086 5182
XmaI C'CCGG_G
Cuts at: 1870 3221 6887 9593 1870
Size: 1351 3666 2706 4545
Fragments arranged by size:
4545 3666 2706 1351
XmnI GAAnn'nnTTC
Cuts at: 4769 10397 4769
Size: 5628 6640
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4396 7882 10076 276
Size: 53 83 186 936 2862 3486 2194 2468
Fragments arranged by size:
3486 2862 2468 2194 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI NsiI PacI SrfI SwaI