Restriction map for
pFN21AA0232
(Circular) (Six-base) MAPSORT of: pFN21AA0232.seq Check: 7287 from: 1 to: 8890
pFN21AA0232 8890 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3891 3891
Size: 8890
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6700 278
Size: 53 83 186 936 5164 2468
Fragments arranged by size:
5164 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6233 1937
Size: 4296 4594
Acc65I G'GTAC_C
Cuts at: 6211 6211
Size: 8890
AclI AA'CG_TT
Cuts at: 1563 7017 7390 1563
Size: 5454 373 3063
Fragments arranged by size:
5454 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 2862 3168 4601 4617 5205 6953 8337
Size: 779 451 306 1433 16 588 1748 1384
Cuts at: 8337 8605 1632
Size: 268 1917
Fragments arranged by size:
1917 1748 1433 1384 779 588 451 306
268 16
AflII C'TTAA_G
Cuts at: 820 1017 5134 820
Size: 197 4117 4576
Fragments arranged by size:
4576 4117 197
AflIII A'CryG_T
Cuts at: 1203 4163 7789 8766 1203
Size: 2960 3626 977 1327
Fragments arranged by size:
3626 2960 1327 977
AgeI A'CCGG_T
Cuts at: 7721 7721
Size: 8890
AhdI GACnn_n'nnGTC
Cuts at: 7619 7619
Size: 8890
AleI CACnn'nnGTG
Cuts at: 1166 2729 1166
Size: 1563 7327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3673 1184
Size: 2489 6401
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4123 4155 4123
Size: 32 8858
AlwNI CAG_nnn'CTG
Cuts at: 2075 4895 6633 8205 2075
Size: 2820 1738 1572 2760
Fragments arranged by size:
2820 2760 1738 1572
ApaI G_GGCC'C
Cuts at: 6155 6155
Size: 8890
ApaLI G'TGCA_C
Cuts at: 6947 8103 6947
Size: 1156 7734
ApoI r'AATT_y
Cuts at: 1493 2777 4414 4510 5378 5525 6199 6425 6577
Size: 1284 1637 96 868 147 674 226 152
Cuts at: 6577 8686 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1637 1284 868 674 226 152
147 96
AseI AT'TA_AT
Cuts at: 160 1032 3918 4230 5724 7444 160
Size: 872 2886 312 1494 1720 1606
Fragments arranged by size:
2886 1720 1606 1494 872 312
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8890
AvaI C'yCGr_G
Cuts at: 1870 1943 2144 6215 1870
Size: 73 201 4071 4545
Fragments arranged by size:
4545 4071 201 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4908 4941 4908
Size: 33 8857
BamHI G'GATC_C
Cuts at: 3562 6220 6587 3562
Size: 2658 367 5865
Fragments arranged by size:
5865 2658 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3569 6211 7666 618
Size: 325 217 627 1782 2642 1455 1842
Fragments arranged by size:
2642 1842 1782 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2145 2406 3204 3906 6155 6171 6209 1391
Size: 754 261 798 702 2249 16 38 4072
Fragments arranged by size:
4072 2249 798 754 702 261 38 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2186 2345 2862 5067 6009 928
Size: 971 287 159 517 2205 942 3809
Fragments arranged by size:
3809 2205 971 942 517 287 159
BbvCI CC'TCA_GC
Cuts at: 3732 3732
Size: 8890
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3206 3240 7081 7115 1903
Size: 34 1269 34 3841 34 3678
Fragments arranged by size:
3841 3678 1269 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3283 6782 7992 3283
Size: 3499 1210 4181
Fragments arranged by size:
4181 3499 1210
BclI T'GATC_A
Cuts at: 1548 3547 5002 1548
Size: 1999 1455 5436
Fragments arranged by size:
5436 1999 1455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7501 136
Size: 107 122 71 631 605 5829 1525
Fragments arranged by size:
5829 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2308 5452 8885 2308
Size: 3144 3433 2313
Fragments arranged by size:
3433 3144 2313
BlpI GC'TnA_GC
Cuts at: 6307 6307
Size: 8890
Bme1580I G_kGCm'C
Cuts at: 1790 4962 6155 6951 8107 1790
Size: 3172 1193 796 1156 2573
Fragments arranged by size:
3172 2573 1193 1156 796
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3465 5202 5610
Size: 416 53 83 399 24 2048 1737 408
Cuts at: 5610 5973 6621 7579 442
Size: 363 648 958 1753
Fragments arranged by size:
2048 1753 1737 958 648 416 408 399
363 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2652 1056
Size: 1596 7294
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 6142 2425
Size: 3717 5173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2395 2463 2484 2863 3819 4628 4703 6078
Size: 1156 68 21 379 956 809 75 1375
Cuts at: 6078 6079 7550 1239
Size: 1 1471 2579
Fragments arranged by size:
2579 1471 1375 1156 956 809 379 75
68 21 1
Bpu10I CC'TnA_GC
Cuts at: 3732 4468 3732
Size: 736 8154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2041 2878 5406 5534 7015 7880 8178 2041
Size: 837 2528 128 1481 865 298 2753
Fragments arranged by size:
2753 2528 1481 865 837 298 128
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2475 3100 3260 4604 7553 882
Size: 617 976 625 160 1344 2949 2219
Fragments arranged by size:
2949 2219 1344 976 625 617 160
BsaAI yAC'GTr
Cuts at: 493 1204 6681 493
Size: 711 5477 2702
Fragments arranged by size:
5477 2702 711
BsaBI GATnn'nnATC
Cuts at: 3507 3546 8884 3507
Size: 39 5338 3513
Fragments arranged by size:
5338 3513 39
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6697 7079 7714 275
Size: 53 83 186 936 5164 382 635 1451
Fragments arranged by size:
5164 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3797 7323 7721 7995 8142 8553 3797
Size: 3526 398 274 147 411 4134
Fragments arranged by size:
4134 3526 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2650 2680 3191 3221 1178
Size: 30 165 30 1247 30 511 30 6847
Fragments arranged by size:
6847 1247 511 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 2414 3212 3840 4812 5304 5376 5877
Size: 858 370 798 628 972 492 72 501
Cuts at: 5877 6179 1186
Size: 302 3897
Fragments arranged by size:
3897 972 858 798 628 501 492 370
302 72
BseYI C'CCAG_C
Cuts at: 2131 2256 8093 8589 2131
Size: 125 5837 496 2432
Fragments arranged by size:
5837 2432 496 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2141 4908 1163
Size: 573 405 2767 5145
Fragments arranged by size:
5145 2767 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6340 7101 8129 1540
Size: 275 186 4339 761 1028 2301
Fragments arranged by size:
4339 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2406 3146 3873 3906 5569 6095 6171 6951
Size: 261 740 727 33 1663 526 76 780
Cuts at: 6951 7036 8107 2145
Size: 85 1071 2928
Fragments arranged by size:
2928 1663 1071 780 740 727 526 261
85 76 33
BsmI GAATG_Cn'
Cuts at: 5174 6401 6494 5174
Size: 1227 93 7570
Fragments arranged by size:
7570 1227 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2406 3146 3204 3873 3906 4962
Size: 399 355 261 740 58 669 33 1056
Cuts at: 4962 5569 6095 6155 6171 6209 6951 7036 8107
Size: 607 526 60 16 38 742 85 1071
Cuts at: 8107 1391
Size: 2174
Fragments arranged by size:
2174 1071 1056 742 740 669 607 526
399 355 261 85 60 58 38 33
16
BspEI T'CCGG_A
Cuts at: 8553 8553
Size: 8890
BspHI T'CATG_A
Cuts at: 2577 6779 8509 2577
Size: 4202 1730 2958
Fragments arranged by size:
4202 2958 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2232 3380 3891 5738 6245 844
Size: 1388 1148 511 1847 507 3489
Fragments arranged by size:
3489 1847 1388 1148 511 507
BsrBI CCG'CTC
Cuts at: 1394 1823 6777 1394
Size: 429 4954 3507
Fragments arranged by size:
4954 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3971 7385 7559 65
Size: 1183 2723 3414 174 1396
Fragments arranged by size:
3414 2723 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7534 7721 1700
Size: 110 5724 187 2869
Fragments arranged by size:
5724 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2018 2814 3162 6038 96
Size: 1922 796 348 2876 2948
Fragments arranged by size:
2948 2876 1922 796 348
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8890
BssSI C'ACGA_G
Cuts at: 2863 2961 6950 7962 2863
Size: 98 3989 1012 3791
Fragments arranged by size:
3989 3791 1012 98
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8890
BstBI TT'CG_AA
Cuts at: 2971 7694 2971
Size: 4723 4167
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8890
BstXI CCAn_nnnn'nTGG
Cuts at: 5498 5498
Size: 8890
BstYI r'GATC_y
Cuts at: 1972 2308 3133 3562 3926 5098 5307 5440 5452
Size: 336 825 429 364 1172 209 133 12
Cuts at: 5452 6220 6587 6972 6989 8441 8527 8539 8885
Size: 768 367 385 17 1452 86 12 346
Cuts at: 8885 1972
Size: 1977
Fragments arranged by size:
1977 1452 1172 825 768 429 385 367
364 346 336 209 133 86 17 12
12
Bsu36I CC'TnA_GG
Cuts at: 2670 2670
Size: 8890
BtgI C'CryG_G
Cuts at: 513 1065 4142 6079 513
Size: 552 3077 1937 3324
Fragments arranged by size:
3324 3077 1937 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8151
Fragments arranged by size:
8151 683 56
BtsI GCAGTG_nn'
Cuts at: 2719 3076 3321 6406 7200 7220 2719
Size: 357 245 3085 794 20 4389
Fragments arranged by size:
4389 3085 794 357 245 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4497 4532 529
Size: 35 3933 35 4887
Fragments arranged by size:
4887 3933 35 35
DraI TTT'AAA
Cuts at: 3114 5215 6194 3114
Size: 2101 979 5810
Fragments arranged by size:
5810 2101 979
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7897 809
Size: 507 6581 1802
Fragments arranged by size:
6581 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6337 7226 8855
Size: 54 1425 50 275 18 4507 889 1629
Cuts at: 8855 8
Size: 43
Fragments arranged by size:
4507 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6337 1537
Size: 275 4525 4090
Fragments arranged by size:
4525 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2436 2626 5006 5796 5982 6111 6156 6820
Size: 1083 190 2380 790 186 129 45 664
Cuts at: 6820 1353
Size: 3423
Fragments arranged by size:
3423 2380 1083 790 664 190 186 129
45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3001 6150 7477 7851 7997 387
Size: 1064 138 1412 3149 1327 374 146 1280
Fragments arranged by size:
3149 1412 1327 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2143 2404 3904 6169 2143
Size: 261 1500 2265 4864
Fragments arranged by size:
4864 2265 1500 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2395 2411 2463 2484 2862 2863 3168
Size: 393 763 16 52 21 378 1 305
Cuts at: 3168 3819 4601 4617 4628 4703 5205 6078 6079
Size: 651 782 16 11 75 502 873 1
Cuts at: 6079 6953 7550 8337 8605 1239
Size: 874 597 787 268 1524
Fragments arranged by size:
1524 874 873 787 782 763 651 597
502 393 378 305 268 75 52 21
16 16 11 1 1
EcoO109I rG'GnC_Cy
Cuts at: 2072 3459 3751 6152 2072
Size: 1387 292 2401 4810
Fragments arranged by size:
4810 2401 1387 292
EcoRI G'AATT_C
Cuts at: 6199 6577 6199
Size: 378 8512
FspI TGC'GCA
Cuts at: 7396 7396
Size: 8890
HaeII r_GCGC'y
Cuts at: 1678 5995 6102 8037 1678
Size: 4317 107 1935 2531
Fragments arranged by size:
4317 2531 1935 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2127 2159 2161 2193 2360
Size: 32 135 32 555 32 2 32 167
Cuts at: 2360 2392 2648 2680 2842 2874 3118 3150 3290
Size: 32 256 32 162 32 244 32 140
Cuts at: 3290 3322 5299 5331 5501 5533 7531 7563 7605
Size: 32 1977 32 170 32 1998 32 42
Cuts at: 7605 7637 1373
Size: 32 2626
Fragments arranged by size:
2626 1998 1977 555 256 244 170 167
162 140 135 42 32 32 32 32
32 32 32 32 32 32 32 32
32 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5281 6234 6480 669
Size: 988 281 3343 953 246 3079
Fragments arranged by size:
3343 3079 988 953 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8890
HpaI GTT'AAC
Cuts at: 6480 6480
Size: 8890
KpnI G_GTAC'C
Cuts at: 6215 6215
Size: 8890
MfeI C'AATT_G
Cuts at: 2793 3222 6489 2793
Size: 429 3267 5194
Fragments arranged by size:
5194 3267 429
MluI A'CGCG_T
Cuts at: 8766 8766
Size: 8890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2854 3540 3578 8004 8188 1305
Size: 1549 686 38 4426 184 2007
Fragments arranged by size:
4426 2007 1549 686 184 38
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7068
Fragments arranged by size:
7068 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2582 2729 3531 4432 4455 5496 5905
Size: 648 1416 147 802 901 23 1041 409
Cuts at: 5905 6848 7207 7366 518
Size: 943 359 159 2042
Fragments arranged by size:
2042 1416 1041 943 901 802 648 409
359 159 147 23
MspA1I CmG'CkG
Cuts at: 1606 3088 5797 6081 6306 6983 8131 8376 1606
Size: 1482 2709 284 225 677 1148 245 2120
Fragments arranged by size:
2709 2120 1482 1148 677 284 245 225
NcoI C'CATG_G
Cuts at: 513 1065 4142 513
Size: 552 3077 5261
Fragments arranged by size:
5261 3077 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8890
NheI G'CTAG_C
Cuts at: 1052 2648 1052
Size: 1596 7294
NotI GC'GGCC_GC
Cuts at: 6337 6337
Size: 8890
NspI r_CATG'y
Cuts at: 1132 1229 6248 7793 1132
Size: 97 5019 1545 2229
Fragments arranged by size:
5019 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4755 1348
Size: 3407 5483
PciI A'CATG_T
Cuts at: 7789 7789
Size: 8890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8890
PfoI T'CCnGG_A
Cuts at: 5101 5101
Size: 8890
PmeI GTTT'AAAC
Cuts at: 6194 6194
Size: 8890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6959 6991 8472 8504 6959
Size: 32 1481 32 7345
Fragments arranged by size:
7345 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3459 3751 3459
Size: 292 8598
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8890
PsiI TTA'TAA
Cuts at: 2274 3434 6460 2274
Size: 1160 3026 4704
Fragments arranged by size:
4704 3026 1160
PspOMI G'GGCC_C
Cuts at: 6151 6151
Size: 8890
PstI C_TGCA'G
Cuts at: 830 2229 3430 3750 6242 830
Size: 1399 1201 320 2492 3478
Fragments arranged by size:
3478 2492 1399 1201 320
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8890
PvuII CAG'CTG
Cuts at: 3088 5797 3088
Size: 2709 6181
SacI G_AGCT'C
Cuts at: 2145 2406 3906 6171 2145
Size: 261 1500 2265 4864
Fragments arranged by size:
4864 2265 1500 261
SacII CC_GC'GG
Cuts at: 6082 6082
Size: 8890
SalI G'TCGA_C
Cuts at: 1936 6232 1936
Size: 4296 4594
SapI GCTCTTCn'nnn_
Cuts at: 2626 5982 2626
Size: 3356 5534
SbfI CC_TGCA'GG
Cuts at: 2229 3750 6242 2229
Size: 1521 2492 4877
Fragments arranged by size:
4877 2492 1521
ScaI AGT'ACT
Cuts at: 7138 8850 7138
Size: 1712 7178
SexAI A'CCwGG_T
Cuts at: 5861 5861
Size: 8890
SfcI C'TryA_G
Cuts at: 826 1045 2225 3426 3746 4462 4521 6238 7373
Size: 219 1180 1201 320 716 59 1717 1135
Cuts at: 7373 8054 8245 826
Size: 681 191 1471
Fragments arranged by size:
1717 1471 1201 1180 1135 716 681 320
219 191 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8890
SmaI CCC'GGG
Cuts at: 1872 6217 1872
Size: 4345 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2056 2144 2893 5134 5421 5513
Size: 197 926 113 88 749 2241 287 92
Cuts at: 5513 6994 7895 8157 820
Size: 1481 901 262 1553
Fragments arranged by size:
2241 1553 1481 926 901 749 287 262
197 113 92 88
SnaBI TAC'GTA
Cuts at: 493 6681 493
Size: 6188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8890
SphI G_CATG'C
Cuts at: 1132 6248 1132
Size: 5116 3774
SspI AAT'ATT
Cuts at: 5 52 3233 4189 5407 6814 5
Size: 47 3181 956 1218 1407 2081
Fragments arranged by size:
3181 2081 1407 1218 956 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8890
StyI C'CwwG_G
Cuts at: 513 1065 2700 3754 4142 5931 6329 513
Size: 552 1635 1054 388 1789 398 3074
Fragments arranged by size:
3074 1789 1635 1054 552 398 388
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5992 6136 6917 6934 7087 7272 7717
Size: 1255 4412 144 781 17 153 185 445
Cuts at: 7717 325
Size: 1498
Fragments arranged by size:
4412 1498 1255 781 445 185 153 144
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2018 2523 2814
Size: 274 80 33 51 1443 41 505 291
Cuts at: 2814 3162 5880 6038 7136 8848 96
Size: 348 2718 158 1098 1712 138
Fragments arranged by size:
2718 1712 1443 1098 505 348 291 274
158 138 80 51 41 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 5260 5292 8850 8882 3191
Size: 32 2037 32 3558 32 3199
Fragments arranged by size:
3558 3199 2037 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8890
XbaI T'CTAG_A
Cuts at: 2317 4061 4279 4588 6226 2317
Size: 1744 218 309 1638 4981
Fragments arranged by size:
4981 1744 1638 309 218
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2124 2124
Size: 8890
XhoI C'TCGA_G
Cuts at: 1943 2144 1943
Size: 201 8689
XmaI C'CCGG_G
Cuts at: 1870 6215 1870
Size: 4345 4545
XmnI GAAnn'nnTTC
Cuts at: 2535 4275 4979 5256 7019 2535
Size: 1740 704 277 1763 4406
Fragments arranged by size:
4406 1763 1740 704 277
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6698 276
Size: 53 83 186 936 5164 2468
Fragments arranged by size:
5164 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SanDI SfiI SfoI SrfI SwaI