Restriction map for
pFN21AA0229
(Circular) (Six-base) MAPSORT of: pFN21AA0229.seq Check: 5459 from: 1 to: 8107
pFN21AA0229 8107 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2823 4574 5917 278
Size: 53 83 186 936 1287 1751 1343 2468
Fragments arranged by size:
2468 1751 1343 1287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5450 1937
Size: 3513 4594
Acc65I G'GTAC_C
Cuts at: 5428 5428
Size: 8107
AclI AA'CG_TT
Cuts at: 1563 5406 6234 6607 1563
Size: 3843 828 373 3063
Fragments arranged by size:
3843 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3090 4466 4687 6170 7554 7822 1632
Size: 1458 1376 221 1483 1384 268 1917
Fragments arranged by size:
1917 1483 1458 1384 1376 268 221
AfeI AGC'GCT
Cuts at: 4700 4700
Size: 8107
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7910
AflIII A'CryG_T
Cuts at: 1203 7006 7983 1203
Size: 5803 977 1327
Fragments arranged by size:
5803 1327 977
AgeI A'CCGG_T
Cuts at: 5269 6938 5269
Size: 1669 6438
AhdI GACnn_n'nnGTC
Cuts at: 6836 6836
Size: 8107
AleI CACnn'nnGTG
Cuts at: 1166 2492 3780 1166
Size: 1326 1288 5493
Fragments arranged by size:
5493 1326 1288
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2280 2771 2781 1184
Size: 1096 491 10 6510
Fragments arranged by size:
6510 1096 491 10
AlwNI CAG_nnn'CTG
Cuts at: 2023 2816 3038 3302 4216 5850 7422 2023
Size: 793 222 264 914 1634 1572 2708
Fragments arranged by size:
2708 1634 1572 914 793 264 222
ApaLI G'TGCA_C
Cuts at: 6164 7320 6164
Size: 1156 6951
ApoI r'AATT_y
Cuts at: 1493 2546 5416 5642 5794 7903 1493
Size: 1053 2870 226 152 2109 1697
Fragments arranged by size:
2870 2109 1697 1053 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2906 6661 160
Size: 872 1874 3755 1606
Fragments arranged by size:
3755 1874 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8107
AvaI C'yCGr_G
Cuts at: 1870 1943 3345 4689 5283 5432 1870
Size: 73 1402 1344 594 149 4545
Fragments arranged by size:
4545 1402 1344 594 149 73
AvrII C'CTAG_G
Cuts at: 3212 3323 3212
Size: 111 7996
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5047 5080 5047
Size: 33 8074
BamHI G'GATC_C
Cuts at: 4707 5437 5804 4707
Size: 730 367 7010
Fragments arranged by size:
7010 730 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2046 3608 4548 4653 4681
Size: 325 217 627 259 1562 940 105 28
Cuts at: 4681 5228 5272 5428 6883 618
Size: 547 44 156 1455 1842
Fragments arranged by size:
1842 1562 1455 940 627 547 325 259
217 156 105 44 28
BanII G_rGCy'C
Cuts at: 1391 4434 4850 5426 1391
Size: 3043 416 576 4072
Fragments arranged by size:
4072 3043 576 416
BbeI G_GCGC'C
Cuts at: 3612 4657 5232 3612
Size: 1045 575 6487
Fragments arranged by size:
6487 1045 575
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3270 3360 928
Size: 971 1371 90 5675
Fragments arranged by size:
5675 1371 971 90
BbvCI CC'TCA_GC
Cuts at: 3177 3789 4544 5396 3177
Size: 612 755 852 5888
Fragments arranged by size:
5888 852 755 612
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4114 4148 6298 6332 1903
Size: 34 2177 34 2150 34 3678
Fragments arranged by size:
3678 2177 2150 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5999 7209 5999
Size: 1210 6897
BclI T'GATC_A
Cuts at: 1548 4857 1548
Size: 3309 4798
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4336 5204 6718
Size: 107 122 71 631 605 2664 868 1514
Cuts at: 6718 136
Size: 1525
Fragments arranged by size:
2664 1525 1514 868 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4074 4618 4864 8102 4074
Size: 544 246 3238 4079
Fragments arranged by size:
4079 3238 544 246
BlpI GC'TnA_GC
Cuts at: 2198 5524 2198
Size: 3326 4781
Bme1580I G_kGCm'C
Cuts at: 1790 2049 3682 4332 6168 7324 1790
Size: 259 1633 650 1836 1156 2573
Fragments arranged by size:
2573 1836 1633 1156 650 259
BmgBI CAC'GTC
Cuts at: 1318 3022 1318
Size: 1704 6403
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3669 4614 4769
Size: 416 53 83 399 24 2252 945 155
Cuts at: 4769 5838 6796 442
Size: 1069 958 1753
Fragments arranged by size:
2252 1753 1069 958 945 416 399 155
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3517 3517
Size: 8107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2173 2609 2968 3216 3222 3656 3681
Size: 809 125 436 359 248 6 434 25
Cuts at: 3681 4115 4139 4247 4617 6767 1239
Size: 434 24 108 370 2150 2579
Fragments arranged by size:
2579 2150 809 436 434 434 370 359
248 125 108 25 24 6
Bpu10I CC'TnA_GC
Cuts at: 2630 3177 3789 4544 5277 5396 2630
Size: 547 612 755 733 119 5341
Fragments arranged by size:
5341 755 733 612 547 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6232 7097 7395 6232
Size: 865 298 6944
Fragments arranged by size:
6944 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2546 3313 3792 6770 882
Size: 617 1047 767 479 2978 2219
Fragments arranged by size:
2978 2219 1047 767 617 479
BsaAI yAC'GTr
Cuts at: 493 1204 5898 493
Size: 711 4694 2702
Fragments arranged by size:
4694 2702 711
BsaBI GATnn'nnATC
Cuts at: 8101 8101
Size: 8107
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2820 3609 4571 4654
Size: 53 83 186 936 1287 789 962 83
Cuts at: 4654 4893 5229 5914 6296 6931 275
Size: 239 336 685 382 635 1451
Fragments arranged by size:
1451 1287 962 936 789 685 635 382
336 239 186 83 83 53
BsaWI w'CCGG_w
Cuts at: 3801 5022 5269 6540 6938 7212 7359 7770 3801
Size: 1221 247 1271 398 274 147 411 4138
Fragments arranged by size:
4138 1271 1221 411 398 274 247 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2171 2201 3143 3173 3506
Size: 30 165 30 768 30 942 30 333
Cuts at: 3506 3536 3754 3784 3922 3952 1178
Size: 30 218 30 138 30 5333
Fragments arranged by size:
5333 942 768 333 218 165 138 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 2528 2696 3500 3511 3653 4523 4730
Size: 894 448 168 804 11 142 870 207
Cuts at: 4730 1186
Size: 4563
Fragments arranged by size:
4563 894 870 804 448 207 168 142
11
BseYI C'CCAG_C
Cuts at: 2967 3600 3669 4539 5164 7310 7806 2967
Size: 633 69 870 625 2146 496 3268
Fragments arranged by size:
3268 2146 870 633 625 496 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2240 2490 2645 3360 4585 4799 1163
Size: 573 504 250 155 715 1225 214 4471
Fragments arranged by size:
4471 1225 715 573 504 250 214 155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4613 5557 6318 7346 1540
Size: 275 186 2612 944 761 1028 2301
Fragments arranged by size:
2612 2301 1028 944 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2508 4434 4722 4926 5016 6168 6253 7324 2508
Size: 1926 288 204 90 1152 85 1071 3291
Fragments arranged by size:
3291 1926 1152 1071 288 204 90 85
BsiWI C'GTAC_G
Cuts at: 4503 4503
Size: 8107
BsmI GAATG_Cn'
Cuts at: 5618 5711 5618
Size: 93 8014
BsmBI CGTCTCn'nnnn_
Cuts at: 3635 3635
Size: 8107
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2508 3682 4332 4434 4722 4850
Size: 399 259 459 1174 650 102 288 128
Cuts at: 4850 4926 5016 5426 6168 6253 7324 1391
Size: 76 90 410 742 85 1071 2174
Fragments arranged by size:
2174 1174 1071 742 650 459 410 399
288 259 128 102 90 85 76
BspEI T'CCGG_A
Cuts at: 5022 7770 5022
Size: 2748 5359
BspHI T'CATG_A
Cuts at: 4026 5996 7726 4026
Size: 1970 1730 4407
Fragments arranged by size:
4407 1970 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3205 3475 3908 4224 5097 5462 844
Size: 2361 270 433 316 873 365 3489
Fragments arranged by size:
3489 2361 873 433 365 316 270
BsrBI CCG'CTC
Cuts at: 1394 1823 3738 4694 5994 1394
Size: 429 1915 956 1300 3507
Fragments arranged by size:
3507 1915 1300 956 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5004 6602 6776 65
Size: 1183 3756 1598 174 1396
Fragments arranged by size:
3756 1598 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4248 5269 6751 6938 1700
Size: 110 2438 1021 1482 187 2869
Fragments arranged by size:
2869 2438 1482 1021 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8107
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8107
BssSI C'ACGA_G
Cuts at: 5015 6167 7179 5015
Size: 1152 1012 5943
Fragments arranged by size:
5943 1152 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2023 2800 4216 794
Size: 1229 777 1416 4685
Fragments arranged by size:
4685 1416 1229 777
BstBI TT'CG_AA
Cuts at: 5178 6911 5178
Size: 1733 6374
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8107
BstXI CCAn_nnnn'nTGG
Cuts at: 3682 4843 3682
Size: 1161 6946
BstYI r'GATC_y
Cuts at: 1972 4074 4618 4707 4864 4935 5019 5437 5804
Size: 2102 544 89 157 71 84 418 367
Cuts at: 5804 6189 6206 7658 7744 7756 8102 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2102 1977 1452 544 418 385 367 346
157 89 86 84 71 17 12
Bsu36I CC'TnA_GG
Cuts at: 2092 2092
Size: 8107
BtgI C'CryG_G
Cuts at: 513 1065 2004 2691 2839 3514 3924 5127 513
Size: 552 939 687 148 675 410 1203 3493
Fragments arranged by size:
3493 1203 939 687 675 552 410 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4451 4465 487
Size: 683 56 3225 14 4129
Fragments arranged by size:
4129 3225 683 56 14
BtsI GCAGTG_nn'
Cuts at: 2755 3039 5623 6417 6437 2755
Size: 284 2584 794 20 4425
Fragments arranged by size:
4425 2584 794 284 20
ClaI AT'CG_AT
Cuts at: 1554 4983 1554
Size: 3429 4678
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3381 3416 5090 5125 529
Size: 35 2817 35 1674 35 3511
Fragments arranged by size:
3511 2817 1674 35 35 35
DraI TTT'AAA
Cuts at: 5411 5411
Size: 8107
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3485 7114 809
Size: 507 2169 3629 1802
Fragments arranged by size:
3629 2169 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2275 2482 2680
Size: 54 1425 50 275 18 445 207 198
Cuts at: 2680 3132 3574 4462 5554 6443 8072 8
Size: 452 442 888 1092 889 1629 43
Fragments arranged by size:
1629 1425 1092 889 888 452 445 442
275 207 198 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5554 1537
Size: 275 3742 4090
Fragments arranged by size:
4090 3742 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2176 3069 3249 4200 6037 1353
Size: 823 893 180 951 1837 3423
Fragments arranged by size:
3423 1837 951 893 823 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3451 4267 6694 7068 7214 387
Size: 1064 138 1862 816 2427 374 146 1280
Fragments arranged by size:
2427 1862 1280 1064 816 374 146 138
EcoICRI GAG'CTC
Cuts at: 4432 4432
Size: 8107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2173 2609 2968 3090 3216 3222
Size: 393 416 125 436 359 122 126 6
Cuts at: 3222 3656 3681 4115 4139 4247 4466 4617 4687
Size: 434 25 434 24 108 219 151 70
Cuts at: 4687 6170 6767 7554 7822 1239
Size: 1483 597 787 268 1524
Fragments arranged by size:
1524 1483 787 597 436 434 434 416
393 359 268 219 151 126 125 122
108 70 25 24 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4283 4283
Size: 8107
EcoO109I rG'GnC_Cy
Cuts at: 2411 2701 3080 3487 3547 3594 4213 5047 5056
Size: 290 379 407 60 47 619 834 9
Cuts at: 5056 5086 2411
Size: 30 5432
Fragments arranged by size:
5432 834 619 407 379 290 60 47
30 9
EcoRI G'AATT_C
Cuts at: 5416 5794 5416
Size: 378 7729
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 8107
FspI TGC'GCA
Cuts at: 2537 6613 2537
Size: 4076 4031
HaeII r_GCGC'y
Cuts at: 1678 3612 3827 4657 4702 5232 7254 1678
Size: 1934 215 830 45 530 2022 2531
Fragments arranged by size:
2531 2022 1934 830 530 215 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2984 3016 3143 3175 3296
Size: 32 135 32 1412 32 127 32 121
Cuts at: 3296 3328 3932 3964 4547 4579 4849 4881 5324
Size: 32 604 32 583 32 270 32 443
Cuts at: 5324 5356 6748 6780 6822 6854 1373
Size: 32 1392 32 42 32 2626
Fragments arranged by size:
2626 1412 1392 604 583 443 270 135
127 121 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2233 2824 3381 5451 5697 669
Size: 988 281 295 591 557 2070 246 3079
Fragments arranged by size:
3079 2070 988 591 557 295 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8107
HpaI GTT'AAC
Cuts at: 3381 5697 3381
Size: 2316 5791
KasI G'GCGC_C
Cuts at: 3608 4653 5228 3608
Size: 1045 575 6487
Fragments arranged by size:
6487 1045 575
KpnI G_GTAC'C
Cuts at: 5432 5432
Size: 8107
MfeI C'AATT_G
Cuts at: 5706 5706
Size: 8107
MluI A'CGCG_T
Cuts at: 7983 7983
Size: 8107
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3102 3914 3975 5016 7221 7405 1305
Size: 1797 812 61 1041 2205 184 2007
Fragments arranged by size:
2205 2007 1797 1041 812 184 61
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2277 2484 2682 3134 3576
Size: 54 1425 343 445 207 198 452 442
Cuts at: 3576 10
Size: 4541
Fragments arranged by size:
4541 1425 452 445 442 343 207 198
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2283 2492 3336 3366 3780 5349 6065
Size: 648 1117 209 844 30 414 1569 716
Cuts at: 6065 6424 6583 518
Size: 359 159 2042
Fragments arranged by size:
2042 1569 1117 844 716 648 414 359
209 159 30
MspA1I CmG'CkG
Cuts at: 1606 2145 3138 3559 3572 3634 4456 4543 5240
Size: 539 993 421 13 62 822 87 697
Cuts at: 5240 5523 6200 7348 7593 1606
Size: 283 677 1148 245 2120
Fragments arranged by size:
2120 1148 993 822 697 677 539 421
283 245 87 62 13
NarI GG'CG_CC
Cuts at: 3609 4654 5229 3609
Size: 1045 575 6487
Fragments arranged by size:
6487 1045 575
NcoI C'CATG_G
Cuts at: 513 1065 2004 2839 3924 513
Size: 552 939 835 1085 4696
Fragments arranged by size:
4696 1085 939 835 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8107
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8107
NotI GC'GGCC_GC
Cuts at: 5554 5554
Size: 8107
NspI r_CATG'y
Cuts at: 1132 1229 2206 3631 3656 5465 7010 1132
Size: 97 977 1425 25 1809 1545 2229
Fragments arranged by size:
2229 1809 1545 1425 977 97 25
PasI CC'CwG_GG
Cuts at: 1348 4348 1348
Size: 3000 5107
PciI A'CATG_T
Cuts at: 7006 7006
Size: 8107
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3121 4186 1108
Size: 2013 1065 5029
Fragments arranged by size:
5029 2013 1065
PfoI T'CCnGG_A
Cuts at: 2075 3658 4710 2075
Size: 1583 1052 5472
Fragments arranged by size:
5472 1583 1052
PmeI GTTT'AAAC
Cuts at: 5411 5411
Size: 8107
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3843 3875 6176 6208 7689 7721 3843
Size: 32 2301 32 1481 32 4229
Fragments arranged by size:
4229 2301 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2701 3080 3487 3547 3594 4213 5047 5056 5086
Size: 379 407 60 47 619 834 9 30
Cuts at: 5086 2701
Size: 5722
Fragments arranged by size:
5722 834 619 407 379 60 47 30
9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8107
PsiI TTA'TAA
Cuts at: 5677 5677
Size: 8107
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5129 5161 5129
Size: 32 8075
PstI C_TGCA'G
Cuts at: 830 2812 3959 4137 4359 5094 5205 5239 5459
Size: 1982 1147 178 222 735 111 34 220
Cuts at: 5459 830
Size: 3478
Fragments arranged by size:
3478 1982 1147 735 222 220 178 111
34
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8107
PvuII CAG'CTG
Cuts at: 3559 3634 4456 4543 5240 3559
Size: 75 822 87 697 6426
Fragments arranged by size:
6426 822 697 87 75
SacI G_AGCT'C
Cuts at: 4434 4434
Size: 8107
SalI G'TCGA_C
Cuts at: 1936 5449 1936
Size: 3513 4594
SanDI GG'GwC_CC
Cuts at: 3080 3594 3080
Size: 514 7593
SapI GCTCTTCn'nnn_
Cuts at: 2176 4200 2176
Size: 2024 6083
SbfI CC_TGCA'GG
Cuts at: 5205 5459 5205
Size: 254 7853
ScaI AGT'ACT
Cuts at: 6355 8067 6355
Size: 1712 6395
SfcI C'TryA_G
Cuts at: 826 1045 2808 3955 4133 4355 5090 5201 5235
Size: 219 1763 1147 178 222 735 111 34
Cuts at: 5235 5455 6590 7271 7462 826
Size: 220 1135 681 191 1471
Fragments arranged by size:
1763 1471 1147 1135 735 681 222 220
219 191 178 111 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 5204 5204
Size: 8107
SfoI GGC'GCC
Cuts at: 3610 4655 5230 3610
Size: 1045 575 6487
Fragments arranged by size:
6487 1045 575
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8107
SgrAI Cr'CCGG_yG
Cuts at: 1700 4248 1700
Size: 2548 5559
SmaI CCC'GGG
Cuts at: 1872 3347 5434 1872
Size: 1475 2087 4545
Fragments arranged by size:
4545 2087 1475
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6211 7112 7374 820
Size: 197 926 4268 901 262 1553
Fragments arranged by size:
4268 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5898 493
Size: 5405 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8107
SphI G_CATG'C
Cuts at: 1132 3631 3656 5465 1132
Size: 2499 25 1809 3774
Fragments arranged by size:
3774 2499 1809 25
SspI AAT'ATT
Cuts at: 5 52 6031 5
Size: 47 5979 2081
Fragments arranged by size:
5979 2081 47
StuI AGG'CCT
Cuts at: 1517 3211 3507 3717 5174 1517
Size: 1694 296 210 1457 4450
Fragments arranged by size:
4450 1694 1457 296 210
StyI C'CwwG_G
Cuts at: 513 1065 2004 2839 3212 3323 3924 4284 5082
Size: 552 939 835 373 111 601 360 798
Cuts at: 5082 5546 513
Size: 464 3074
Fragments arranged by size:
3074 939 835 798 601 552 464 373
360 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2798 3922 6134 6151 6304 6489 6934
Size: 1255 1218 1124 2212 17 153 185 445
Cuts at: 6934 325
Size: 1498
Fragments arranged by size:
2212 1498 1255 1218 1124 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2936 6353 8065
Size: 274 80 33 51 1443 959 3417 1712
Cuts at: 8065 96
Size: 138
Fragments arranged by size:
3417 1712 1443 959 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2171 2203 3506 3538 3754 3786 3922 3954 8067
Size: 32 1303 32 216 32 136 32 4113
Cuts at: 8067 8099 2171
Size: 32 2179
Fragments arranged by size:
4113 2179 1303 216 136 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4175 4364 1316
Size: 2859 189 5059
Fragments arranged by size:
5059 2859 189
XbaI T'CTAG_A
Cuts at: 5443 5443
Size: 8107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3631 4782 3631
Size: 1151 6956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8107
XmaI C'CCGG_G
Cuts at: 1870 3345 5432 1870
Size: 1475 2087 4545
Fragments arranged by size:
4545 2087 1475
XmnI GAAnn'nnTTC
Cuts at: 6236 6236
Size: 8107
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2821 4572 5915 276
Size: 53 83 186 936 1287 1751 1343 2468
Fragments arranged by size:
2468 1751 1343 1287 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BfrBI BstZ17I DraIII EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI RsrII SacII SexAI SrfI SwaI