Restriction map for
pFN21AA0228
(Circular) (Six-base) MAPSORT of: pFN21AA0228.seq Check: 8496 from: 1 to: 6460
pFN21AA0228 6460 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4270 278
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3803 1937
Size: 1866 4594
Acc65I G'GTAC_C
Cuts at: 3781 3781
Size: 6460
AclI AA'CG_TT
Cuts at: 1563 4587 4960 1563
Size: 3024 373 3063
Fragments arranged by size:
3063 3024 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2271 2429 3132 3369 3726 4523 5907 6175
Size: 639 158 703 237 357 797 1384 268
Cuts at: 6175 1632
Size: 1917
Fragments arranged by size:
1917 1384 797 703 639 357 268 237
158
AflII C'TTAA_G
Cuts at: 820 1017 2749 820
Size: 197 1732 4531
Fragments arranged by size:
4531 1732 197
AflIII A'CryG_T
Cuts at: 1203 5359 6336 1203
Size: 4156 977 1327
Fragments arranged by size:
4156 1327 977
AgeI A'CCGG_T
Cuts at: 3636 5291 3636
Size: 1655 4805
AhdI GACnn_n'nnGTC
Cuts at: 5189 5189
Size: 6460
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6460
AlwNI CAG_nnn'CTG
Cuts at: 2342 3208 4203 5775 2342
Size: 866 995 1572 3027
Fragments arranged by size:
3027 1572 995 866
ApaLI G'TGCA_C
Cuts at: 2643 4517 5673 2643
Size: 1874 1156 3430
Fragments arranged by size:
3430 1874 1156
ApoI r'AATT_y
Cuts at: 1493 2180 2786 3050 3272 3335 3769 3995 4147
Size: 687 606 264 222 63 434 226 152
Cuts at: 4147 6256 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 687 606 434 264 226 222
152 63
AseI AT'TA_AT
Cuts at: 160 1032 5014 160
Size: 872 3982 1606
Fragments arranged by size:
3982 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 3785 1870
Size: 73 168 1674 4545
Fragments arranged by size:
4545 1674 168 73
BamHI G'GATC_C
Cuts at: 2028 2846 3790 4157 2028
Size: 818 944 367 4331
Fragments arranged by size:
4331 944 818 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3781 5236 618
Size: 325 217 627 1994 1455 1842
Fragments arranged by size:
1994 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3779 1391
Size: 2388 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3231 3319 928
Size: 971 1332 88 4069
Fragments arranged by size:
4069 1332 971 88
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4651 4685 1903
Size: 34 2714 34 3678
Fragments arranged by size:
3678 2714 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4352 5562 4352
Size: 1210 5250
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6460
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5071 136
Size: 107 122 71 631 605 337 3062 1525
Fragments arranged by size:
3062 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6455 6455
Size: 6460
BlpI GC'TnA_GC
Cuts at: 3877 3877
Size: 6460
Bme1580I G_kGCm'C
Cuts at: 1790 2647 4521 5677 1790
Size: 857 1874 1156 2573
Fragments arranged by size:
2573 1874 1156 857
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6460
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2180 3691 4191
Size: 416 53 83 399 24 763 1511 500
Cuts at: 4191 5149 442
Size: 958 1753
Fragments arranged by size:
1753 1511 958 763 500 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5120 1239
Size: 3881 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4585 5450 5748 4585
Size: 865 298 5297
Fragments arranged by size:
5297 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3566 5123 882
Size: 617 2067 1557 2219
Fragments arranged by size:
2219 2067 1557 617
BsaAI yAC'GTr
Cuts at: 493 1204 2989 4251 493
Size: 711 1785 1262 2702
Fragments arranged by size:
2702 1785 1262 711
BsaBI GATnn'nnATC
Cuts at: 6454 6454
Size: 6460
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4267 4649 5284 275
Size: 53 83 186 936 2734 382 635 1451
Fragments arranged by size:
2734 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3636 4893 5291 5565 5712 6123 3636
Size: 1257 398 274 147 411 3973
Fragments arranged by size:
3973 1257 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2069 2087 2099 2117 1178
Size: 30 165 30 666 18 12 18 5521
Fragments arranged by size:
5521 666 165 30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3179 1186
Size: 1993 4467
BseYI C'CCAG_C
Cuts at: 5663 6159 5663
Size: 496 5964
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2508 2607 3737 1163
Size: 573 772 99 1130 3886
Fragments arranged by size:
3886 1130 772 573 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 3910 4671 5699 1540
Size: 275 186 12 1897 761 1028 2301
Fragments arranged by size:
2301 1897 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2321 2647 4521 4606 5677 2321
Size: 326 1874 85 1071 3104
Fragments arranged by size:
3104 1874 1071 326 85
BsmI GAATG_Cn'
Cuts at: 3971 4064 3971
Size: 93 6367
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2321 2647 3779 4521 4606 5677 1391
Size: 399 531 326 1132 742 85 1071 2174
Fragments arranged by size:
2174 1132 1071 742 531 399 326 85
BspEI T'CCGG_A
Cuts at: 6123 6123
Size: 6460
BspHI T'CATG_A
Cuts at: 3001 3223 4349 6079 3001
Size: 222 1126 1730 3382
Fragments arranged by size:
3382 1730 1126 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3815 844
Size: 2971 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2085 2103 4347 1394
Size: 429 262 18 2244 3507
Fragments arranged by size:
3507 2244 429 262 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 2706 4955 5129 65
Size: 1183 1458 2249 174 1396
Fragments arranged by size:
2249 1458 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3636 5104 5291 1700
Size: 110 1826 1468 187 2869
Fragments arranged by size:
2869 1826 1468 187 110
BsrGI T'GTAC_A
Cuts at: 96 3280 96
Size: 3184 3276
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6460
BssSI C'ACGA_G
Cuts at: 4520 5532 4520
Size: 1012 5448
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6460
BstBI TT'CG_AA
Cuts at: 5264 5264
Size: 6460
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6460
BstYI r'GATC_y
Cuts at: 1972 2028 2846 3790 4157 4542 4559 6011 6097
Size: 56 818 944 367 385 17 1452 86
Cuts at: 6097 6109 6455 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 944 818 385 367 346 86
56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 513
Size: 552 939 9 4960
Fragments arranged by size:
4960 939 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5721
Fragments arranged by size:
5721 683 56
BtsI GCAGTG_nn'
Cuts at: 2182 2826 2873 3976 4770 4790 2182
Size: 644 47 1103 794 20 3852
Fragments arranged by size:
3852 1103 794 644 47 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6460
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6425
DraI TTT'AAA
Cuts at: 2563 3764 2563
Size: 1201 5259
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2163 5467 809
Size: 507 847 3304 1802
Fragments arranged by size:
3304 1802 847 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2602 3907
Size: 54 1425 50 275 18 180 592 1305
Cuts at: 3907 4796 6425 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1305 889 592 275 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 3907 1537
Size: 275 198 1897 4090
Fragments arranged by size:
4090 1897 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 3025 4390 1353
Size: 1672 1365 3423
Fragments arranged by size:
3423 1672 1365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5047 5421 5567 387
Size: 1064 138 3458 374 146 1280
Fragments arranged by size:
3458 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 2429 3132 3369 3726 4523 5120
Size: 393 639 158 703 237 357 797 597
Cuts at: 5120 5907 6175 1239
Size: 787 268 1524
Fragments arranged by size:
1524 797 787 703 639 597 393 357
268 237 158
EcoRI G'AATT_C
Cuts at: 3769 4147 3769
Size: 378 6082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2775 3137 3404 3434 2775
Size: 362 267 30 5801
Fragments arranged by size:
5801 362 267 30
FspI TGC'GCA
Cuts at: 4966 4966
Size: 6460
HaeII r_GCGC'y
Cuts at: 1678 5607 1678
Size: 3929 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2576 2608 3655
Size: 32 135 32 515 32 457 32 1047
Cuts at: 3655 3687 5101 5133 5175 5207 1373
Size: 32 1414 32 42 32 2626
Fragments arranged by size:
2626 1414 1047 515 457 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2168 3804 4050 669
Size: 988 281 230 1636 246 3079
Fragments arranged by size:
3079 1636 988 281 246 230
HindIII A'AGCT_T
Cuts at: 748 2141 2757 3261 748
Size: 1393 616 504 3947
Fragments arranged by size:
3947 1393 616 504
HpaI GTT'AAC
Cuts at: 4050 4050
Size: 6460
KpnI G_GTAC'C
Cuts at: 3785 3785
Size: 6460
MfeI C'AATT_G
Cuts at: 3174 3504 4059 3174
Size: 330 555 5575
Fragments arranged by size:
5575 555 330
MluI A'CGCG_T
Cuts at: 6336 6336
Size: 6460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3271 5574 5758 1305
Size: 1966 2303 184 2007
Fragments arranged by size:
2303 2007 1966 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2604 10
Size: 54 1425 343 772 3866
Fragments arranged by size:
3866 1425 772 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2547 2670 2683 4418 4777 4936 518
Size: 648 1381 123 13 1735 359 159 2042
Fragments arranged by size:
2042 1735 1381 648 359 159 123 13
MspA1I CmG'CkG
Cuts at: 1606 2063 3876 4553 5701 5946 1606
Size: 457 1813 677 1148 245 2120
Fragments arranged by size:
2120 1813 1148 677 457 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4969
Fragments arranged by size:
4969 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6460
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6460
NotI GC'GGCC_GC
Cuts at: 3907 3907
Size: 6460
NspI r_CATG'y
Cuts at: 1132 1229 2819 3818 5363 1132
Size: 97 1590 999 1545 2229
Fragments arranged by size:
2229 1590 1545 999 97
PacI TTA_AT'TAA
Cuts at: 2811 2811
Size: 6460
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6460
PciI A'CATG_T
Cuts at: 5359 5359
Size: 6460
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6460
PmeI GTTT'AAAC
Cuts at: 3764 3764
Size: 6460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4529 4561 6042 6074 4529
Size: 32 1481 32 4915
Fragments arranged by size:
4915 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6460
PsiI TTA'TAA
Cuts at: 4030 4030
Size: 6460
PstI C_TGCA'G
Cuts at: 830 2347 3812 830
Size: 1517 1465 3478
Fragments arranged by size:
3478 1517 1465
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6460
SalI G'TCGA_C
Cuts at: 1936 3802 1936
Size: 1866 4594
SbfI CC_TGCA'GG
Cuts at: 3812 3812
Size: 6460
ScaI AGT'ACT
Cuts at: 2211 4708 6420 2211
Size: 2497 1712 2251
Fragments arranged by size:
2497 2251 1712
SfcI C'TryA_G
Cuts at: 826 1045 2343 3498 3532 3808 4943 5624 5815
Size: 219 1298 1155 34 276 1135 681 191
Cuts at: 5815 826
Size: 1471
Fragments arranged by size:
1471 1298 1155 1135 681 276 219 191
34
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6460
SmaI CCC'GGG
Cuts at: 1872 3787 1872
Size: 1915 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2749 4564 5465 5727 820
Size: 197 926 806 1815 901 262 1553
Fragments arranged by size:
1815 1553 926 901 806 262 197
SnaBI TAC'GTA
Cuts at: 493 4251 493
Size: 3758 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6460
SphI G_CATG'C
Cuts at: 1132 3818 1132
Size: 2686 3774
SspI AAT'ATT
Cuts at: 5 52 2864 3656 4384 5
Size: 47 2812 792 728 2081
Fragments arranged by size:
2812 2081 792 728 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6460
StyI C'CwwG_G
Cuts at: 513 1065 2004 3307 3899 513
Size: 552 939 1303 592 3074
Fragments arranged by size:
3074 1303 939 592 552
SwaI ATTT'AAAT
Cuts at: 2563 2563
Size: 6460
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2537 2871 3766 4487 4504 4657 4842
Size: 1255 957 334 895 721 17 153 185
Cuts at: 4842 5287 325
Size: 445 1498
Fragments arranged by size:
1498 1255 957 895 721 445 334 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2209 2453 3280
Size: 274 80 33 51 1443 232 244 827
Cuts at: 3280 4706 6418 96
Size: 1426 1712 138
Fragments arranged by size:
1712 1443 1426 827 274 244 232 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2009 2041 6420 6452 2009
Size: 32 4379 32 2017
Fragments arranged by size:
4379 2017 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6460
XbaI T'CTAG_A
Cuts at: 3796 3796
Size: 6460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3041 3709 2011
Size: 1030 668 4762
Fragments arranged by size:
4762 1030 668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6460
XmaI C'CCGG_G
Cuts at: 1870 3785 1870
Size: 1915 4545
XmnI GAAnn'nnTTC
Cuts at: 3387 4589 3387
Size: 1202 5258
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4268 276
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PacI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI