Restriction map for
pFN21AA0226
(Circular) (Six-base) MAPSORT of: pFN21AA0226.seq Check: 3021 from: 1 to: 7486
pFN21AA0226 7486 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5296 278
Size: 53 83 186 936 3760 2468
Fragments arranged by size:
3760 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4829 1937
Size: 2892 4594
Acc65I G'GTAC_C
Cuts at: 3237 4807 3237
Size: 1570 5916
AclI AA'CG_TT
Cuts at: 1563 5613 5986 1563
Size: 4050 373 3063
Fragments arranged by size:
4050 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3340 3494 3791 4156 5549 6933 7201 1632
Size: 1708 154 297 365 1393 1384 268 1917
Fragments arranged by size:
1917 1708 1393 1384 365 297 268 154
AfeI AGC'GCT
Cuts at: 4752 4752
Size: 7486
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7289
AflIII A'CryG_T
Cuts at: 1203 4332 6385 7362 1203
Size: 3129 2053 977 1327
Fragments arranged by size:
3129 2053 1327 977
AgeI A'CCGG_T
Cuts at: 6317 6317
Size: 7486
AhdI GACnn_n'nnGTC
Cuts at: 6215 6215
Size: 7486
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7486
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3151 1184
Size: 1967 5519
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 7454
AlwNI CAG_nnn'CTG
Cuts at: 2291 2338 2443 2666 2915 3472 4708 5229 6801
Size: 47 105 223 249 557 1236 521 1572
Cuts at: 6801 2291
Size: 2976
Fragments arranged by size:
2976 1572 1236 557 521 249 223 105
47
ApaI G_GGCC'C
Cuts at: 3952 4464 3952
Size: 512 6974
ApaLI G'TGCA_C
Cuts at: 2138 5543 6699 2138
Size: 3405 1156 2925
Fragments arranged by size:
3405 2925 1156
ApoI r'AATT_y
Cuts at: 1493 2276 3641 4795 5021 5173 7282 1493
Size: 783 1365 1154 226 152 2109 1697
Fragments arranged by size:
2109 1697 1365 1154 783 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6040 160
Size: 872 5008 1606
Fragments arranged by size:
5008 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7486
AvaI C'yCGr_G
Cuts at: 1870 1943 2252 2820 4811 1870
Size: 73 309 568 1991 4545
Fragments arranged by size:
4545 1991 568 309 73
BamHI G'GATC_C
Cuts at: 4816 5183 4816
Size: 367 7119
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 3237 3495 4807 6262
Size: 325 217 627 381 1069 258 1312 1455
Cuts at: 6262 618
Size: 1842
Fragments arranged by size:
1842 1455 1312 1069 627 381 325 258
217
BanII G_rGCy'C
Cuts at: 1391 2417 2711 3082 3768 3952 4464 4479 4587
Size: 1026 294 371 686 184 512 15 108
Cuts at: 4587 4743 4805 1391
Size: 156 62 4072
Fragments arranged by size:
4072 1026 686 512 371 294 184 156
108 62 15
BbeI G_GCGC'C
Cuts at: 3499 3499
Size: 7486
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3274 3453 928
Size: 971 1375 179 4961
Fragments arranged by size:
4961 1375 971 179
BbvCI CC'TCA_GC
Cuts at: 3747 3747
Size: 7486
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3679 3713 5677 5711 1903
Size: 34 50 34 1658 34 1964 34 3678
Fragments arranged by size:
3678 1964 1658 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3049 5378 6588 3049
Size: 2329 1210 3947
Fragments arranged by size:
3947 2329 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7486
BfrBI ATG'CAT
Cuts at: 4512 4512
Size: 7486
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4136 6097 136
Size: 107 122 71 631 605 2464 1961 1525
Fragments arranged by size:
2464 1961 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2558 4218 7481 2558
Size: 1660 3263 2563
Fragments arranged by size:
3263 2563 1660
BlpI GC'TnA_GC
Cuts at: 2490 3543 4473 4903 2490
Size: 1053 930 430 5073
Fragments arranged by size:
5073 1053 930 430
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2538 2616 3952 4264 4464 5547 6703
Size: 352 396 78 1336 312 200 1083 1156
Cuts at: 6703 1790
Size: 2573
Fragments arranged by size:
2573 1336 1156 1083 396 352 312 200
78
BmgBI CAC'GTC
Cuts at: 1318 2617 3741 1318
Size: 1299 1124 5063
Fragments arranged by size:
5063 1299 1124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2451 2648 3574
Size: 416 53 83 399 24 1034 197 926
Cuts at: 3574 3703 5217 6175 442
Size: 129 1514 958 1753
Fragments arranged by size:
1753 1514 1034 958 926 416 399 197
129 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7486
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2695 2875 3055 3066 3553 3833 4730 4796
Size: 1456 180 180 11 487 280 897 66
Cuts at: 4796 6146 1239
Size: 1350 2579
Fragments arranged by size:
2579 1456 1350 897 487 280 180 180
66 11
Bpu10I CC'TnA_GC
Cuts at: 3247 3747 3247
Size: 500 6986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2583 3667 5611 6476 6774 2583
Size: 1084 1944 865 298 3295
Fragments arranged by size:
3295 1944 1084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6149 882
Size: 617 4650 2219
Fragments arranged by size:
4650 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2119 3593 5277 493
Size: 711 915 1474 1684 2702
Fragments arranged by size:
2702 1684 1474 915 711
BsaBI GATnn'nnATC
Cuts at: 4139 7480 4139
Size: 3341 4145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 5293 5675 6310
Size: 53 83 186 936 1963 1797 382 635
Cuts at: 6310 275
Size: 1451
Fragments arranged by size:
1963 1797 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2088 3883 4047 5919 6317 6591 6738 7149 2088
Size: 1795 164 1872 398 274 147 411 2425
Fragments arranged by size:
2425 1872 1795 411 398 274 164 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3029 3059 3800 3830 1178
Size: 30 165 30 1626 30 741 30 4834
Fragments arranged by size:
4834 1626 741 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2878 3440 3443 4748 4751 1186
Size: 1692 562 3 1305 3 3921
Fragments arranged by size:
3921 1692 1305 562 3 3
BseYI C'CCAG_C
Cuts at: 2235 3203 4145 4456 6689 7185 2235
Size: 968 942 311 2233 496 2536
Fragments arranged by size:
2536 2233 968 942 496 311
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2652 3716 4053 4265 1163
Size: 573 916 1064 337 212 4384
Fragments arranged by size:
4384 1064 916 573 337 212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4936 5697 6725 1540
Size: 275 186 2935 761 1028 2301
Fragments arranged by size:
2935 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2142 2711 3082 3248 3549 4479 4587 5547 5632
Size: 569 371 166 301 930 108 960 85
Cuts at: 5632 6703 2142
Size: 1071 2925
Fragments arranged by size:
2925 1071 960 930 569 371 301 166
108 85
BsmI GAATG_Cn'
Cuts at: 2494 3352 4132 4997 5090 2494
Size: 858 780 865 93 4890
Fragments arranged by size:
4890 865 858 780 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2623 2623
Size: 7486
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2417 2538 2616 2711 3082 3248
Size: 399 352 275 121 78 95 371 166
Cuts at: 3248 3549 3768 3952 4264 4464 4479 4587 4743
Size: 301 219 184 312 200 15 108 156
Cuts at: 4743 4805 5547 5632 6703 1391
Size: 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 371 352 312 301
275 219 200 184 166 156 121 108
95 85 78 62 15
BspEI T'CCGG_A
Cuts at: 4047 7149 4047
Size: 3102 4384
BspHI T'CATG_A
Cuts at: 5375 7105 5375
Size: 1730 5756
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2970 3528 4215 4841 844
Size: 2126 558 687 626 3489
Fragments arranged by size:
3489 2126 687 626 558
BsrBI CCG'CTC
Cuts at: 1394 1823 2419 4669 5373 1394
Size: 429 596 2250 704 3507
Fragments arranged by size:
3507 2250 704 596 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2863 3063 3123 3145 5981 6155 65
Size: 1183 1615 200 60 22 2836 174 1396
Fragments arranged by size:
2836 1615 1396 1183 200 174 60 22
BsrFI r'CCGG_y
Cuts at: 1700 1810 6130 6317 1700
Size: 110 4320 187 2869
Fragments arranged by size:
4320 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3213 96
Size: 3117 4369
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7486
BssSI C'ACGA_G
Cuts at: 2141 2408 4121 5546 6558 2141
Size: 267 1713 1425 1012 3069
Fragments arranged by size:
3069 1713 1425 1012 267
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2212 4518 794
Size: 1418 2306 3762
Fragments arranged by size:
3762 2306 1418
BstBI TT'CG_AA
Cuts at: 6290 6290
Size: 7486
BstEII G'GTnAC_C
Cuts at: 1184 3562 1184
Size: 2378 5108
BstYI r'GATC_y
Cuts at: 1972 2558 3118 3462 3483 4218 4816 5183 5568
Size: 586 560 344 21 735 598 367 385
Cuts at: 5568 5585 7037 7123 7135 7481 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 735 598 586 560 385 367
346 344 86 21 17 12
Bsu36I CC'TnA_GG
Cuts at: 2929 2929
Size: 7486
BtgI C'CryG_G
Cuts at: 513 1065 3759 4008 4743 513
Size: 552 2694 249 735 3256
Fragments arranged by size:
3256 2694 735 552 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6747
Fragments arranged by size:
6747 683 56
BtsI GCAGTG_nn'
Cuts at: 2164 2221 2339 2352 3193 3721 3869 5002 5796
Size: 57 118 13 841 528 148 1133 794
Cuts at: 5796 5816 2164
Size: 20 3834
Fragments arranged by size:
3834 1133 841 794 528 148 118 57
20 13
ClaI AT'CG_AT
Cuts at: 1554 2382 1554
Size: 828 6658
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7451
DraI TTT'AAA
Cuts at: 4790 4790
Size: 7486
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2315 6493 809
Size: 507 999 4178 1802
Fragments arranged by size:
4178 1802 999 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 2799 3756
Size: 54 1425 50 275 18 492 477 957
Cuts at: 3756 4011 4137 4353 4695 4933 5822 7451 8
Size: 255 126 216 342 238 889 1629 43
Fragments arranged by size:
1629 1425 957 889 492 477 342 275
255 238 216 126 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4933 1537
Size: 275 3121 4090
Fragments arranged by size:
4090 3121 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2929 3435 4241 4602 5416 1353
Size: 1576 506 806 361 814 3423
Fragments arranged by size:
3423 1576 814 806 506 361
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3476 6073 6447 6593 387
Size: 1064 138 1887 2597 374 146 1280
Fragments arranged by size:
2597 1887 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2709 3080 4477 4585 2709
Size: 371 1397 108 5610
Fragments arranged by size:
5610 1397 371 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2695 2875 3055 3066 3340 3494 3553
Size: 393 1063 180 180 11 274 154 59
Cuts at: 3553 3791 3833 4156 4730 4796 5549 6146 6933
Size: 238 42 323 574 66 753 597 787
Cuts at: 6933 7201 1239
Size: 268 1524
Fragments arranged by size:
1524 1063 787 753 597 574 393 323
274 268 238 180 180 154 66 59
42 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3338 3425 4400 3338
Size: 87 975 6424
Fragments arranged by size:
6424 975 87
EcoO109I rG'GnC_Cy
Cuts at: 2508 2786 3089 3161 3949 4204 4405 4460 4461
Size: 278 303 72 788 255 201 55 1
Cuts at: 4461 2508
Size: 5533
Fragments arranged by size:
5533 788 303 278 255 201 72 55
1
EcoRI G'AATT_C
Cuts at: 4795 5173 4795
Size: 378 7108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2580 4186 2580
Size: 1606 5880
FspI TGC'GCA
Cuts at: 4160 5992 4160
Size: 1832 5654
HaeII r_GCGC'y
Cuts at: 1678 3499 4754 6633 1678
Size: 1821 1255 1879 2531
Fragments arranged by size:
2531 1879 1821 1255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2253 2285 2543 2575 2601
Size: 32 135 32 681 32 258 32 26
Cuts at: 2601 2633 3273 3305 3605 3637 4029 4061 4500
Size: 32 640 32 300 32 392 32 439
Cuts at: 4500 4532 6127 6159 6201 6233 1373
Size: 32 1595 32 42 32 2626
Fragments arranged by size:
2626 1595 681 640 439 392 300 258
135 42 32 32 32 32 32 32
32 32 32 32 32 26
HincII GTy'rAC
Cuts at: 669 1657 1938 4830 5076 669
Size: 988 281 2892 246 3079
Fragments arranged by size:
3079 2892 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3553 748
Size: 2805 4681
HpaI GTT'AAC
Cuts at: 5076 5076
Size: 7486
KasI G'GCGC_C
Cuts at: 3495 3495
Size: 7486
KpnI G_GTAC'C
Cuts at: 3241 4811 3241
Size: 1570 5916
MfeI C'AATT_G
Cuts at: 5085 5085
Size: 7486
MluI A'CGCG_T
Cuts at: 7362 7362
Size: 7486
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6600 6784 1305
Size: 5295 184 2007
Fragments arranged by size:
5295 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2801 3758 4013 4139 4355
Size: 54 1425 343 969 957 255 126 216
Cuts at: 4355 4697 10
Size: 342 2799
Fragments arranged by size:
2799 1425 969 957 343 342 255 216
126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 2324 4125 5444 5803 5962 518
Size: 648 969 189 1801 1319 359 159 2042
Fragments arranged by size:
2042 1801 1319 969 648 359 189 159
MspA1I CmG'CkG
Cuts at: 1606 2838 2962 3324 4029 4315 4745 4902 5579
Size: 1232 124 362 705 286 430 157 677
Cuts at: 5579 6727 6972 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1232 1148 705 677 430 362 286
245 157 124
NarI GG'CG_CC
Cuts at: 3496 3496
Size: 7486
NcoI C'CATG_G
Cuts at: 513 1065 3759 513
Size: 552 2694 4240
Fragments arranged by size:
4240 2694 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7486
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7486
NotI GC'GGCC_GC
Cuts at: 4933 4933
Size: 7486
NsiI A_TGCA'T
Cuts at: 4514 4514
Size: 7486
NspI r_CATG'y
Cuts at: 1132 1229 3262 4336 4516 4844 6389 1132
Size: 97 2033 1074 180 328 1545 2229
Fragments arranged by size:
2229 2033 1545 1074 328 180 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7486
PciI A'CATG_T
Cuts at: 4332 6385 4332
Size: 2053 5433
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2579 1108
Size: 1471 6015
PfoI T'CCnGG_A
Cuts at: 3082 3465 3825 4773 3082
Size: 383 360 948 5795
Fragments arranged by size:
5795 948 383 360
PmeI GTTT'AAAC
Cuts at: 4790 4790
Size: 7486
PmlI CAC'GTG
Cuts at: 2119 3593 2119
Size: 1474 6012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2857 2889 3665 3697 5555 5587 7068 7100 2857
Size: 32 776 32 1858 32 1481 32 3243
Fragments arranged by size:
3243 1858 1481 776 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2508 4204 4405 2508
Size: 1696 201 5589
Fragments arranged by size:
5589 1696 201
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7486
PsiI TTA'TAA
Cuts at: 5056 5056
Size: 7486
PspOMI G'GGCC_C
Cuts at: 3948 4460 3948
Size: 512 6974
PstI C_TGCA'G
Cuts at: 830 2205 2217 2334 4677 4838 830
Size: 1375 12 117 2343 161 3478
Fragments arranged by size:
3478 2343 1375 161 117 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7486
PvuII CAG'CTG
Cuts at: 2838 2962 3324 4315 2838
Size: 124 362 991 6009
Fragments arranged by size:
6009 991 362 124
SacI G_AGCT'C
Cuts at: 2711 3082 4479 4587 2711
Size: 371 1397 108 5610
Fragments arranged by size:
5610 1397 371 108
SacII CC_GC'GG
Cuts at: 4746 4746
Size: 7486
SalI G'TCGA_C
Cuts at: 1936 4828 1936
Size: 2892 4594
SbfI CC_TGCA'GG
Cuts at: 4838 4838
Size: 7486
ScaI AGT'ACT
Cuts at: 2716 4103 4181 5734 7446 2716
Size: 1387 78 1553 1712 2756
Fragments arranged by size:
2756 1712 1553 1387 78
SfcI C'TryA_G
Cuts at: 826 1045 2201 2213 2330 4267 4673 4834 5969
Size: 219 1156 12 117 1937 406 161 1135
Cuts at: 5969 6650 6841 826
Size: 681 191 1471
Fragments arranged by size:
1937 1471 1156 1135 681 406 219 191
161 117 12
SfoI GGC'GCC
Cuts at: 3497 3497
Size: 7486
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7486
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7486
SmaI CCC'GGG
Cuts at: 1872 4813 1872
Size: 2941 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2598 3682 5590 6491 6753 820
Size: 197 926 655 1084 1908 901 262 1553
Fragments arranged by size:
1908 1553 1084 926 901 655 262 197
SnaBI TAC'GTA
Cuts at: 493 5277 493
Size: 4784 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7486
SphI G_CATG'C
Cuts at: 1132 4516 4844 1132
Size: 3384 328 3774
Fragments arranged by size:
3774 3384 328
SspI AAT'ATT
Cuts at: 5 52 5410 5
Size: 47 5358 2081
Fragments arranged by size:
5358 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7486
StyI C'CwwG_G
Cuts at: 513 1065 3759 4200 4356 4925 513
Size: 552 2694 441 156 569 3074
Fragments arranged by size:
3074 2694 569 552 441 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3217 5513 5530 5683 5868 6313 325
Size: 1255 1637 2296 17 153 185 445 1498
Fragments arranged by size:
2296 1637 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2714 3213 4101
Size: 274 80 33 51 1443 737 499 888
Cuts at: 4101 4179 4416 5732 7444 96
Size: 78 237 1316 1712 138
Fragments arranged by size:
1712 1443 1316 888 737 499 274 237
138 80 78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3029 3061 3800 3832 7446 7478 3029
Size: 32 739 32 3614 32 3037
Fragments arranged by size:
3614 3037 739 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4382 1316
Size: 3066 4420
XbaI T'CTAG_A
Cuts at: 4822 4822
Size: 7486
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2809 2945 4363 4705 2809
Size: 136 1418 342 5590
Fragments arranged by size:
5590 1418 342 136
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7486
XmaI C'CCGG_G
Cuts at: 1870 4811 1870
Size: 2941 4545
XmnI GAAnn'nnTTC
Cuts at: 4334 5615 4334
Size: 1281 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5294 276
Size: 53 83 186 936 3760 2468
Fragments arranged by size:
3760 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BplI BsiWI BstXI BstZ17I
DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI
PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI
SwaI