Restriction map for
pFN21AA0213
(Circular) (Six-base) MAPSORT of: pFN21AA0213.seq Check: 7014 from: 1 to: 9379
pFN21AA0213 9379 bp
With 182 enzymes: SgfI *
March 28, 2012 12:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4816 5939 4816
Size: 1123 8256
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7189 278
Size: 53 83 186 936 5653 2468
Fragments arranged by size:
5653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2913 3559 3594 5943 6722 1937
Size: 976 646 35 2349 779 4594
Fragments arranged by size:
4594 2349 976 779 646 35
Acc65I G'GTAC_C
Cuts at: 2249 3415 5464 5778 6700 2249
Size: 1166 2049 314 922 4928
Fragments arranged by size:
4928 2049 1166 922 314
AclI AA'CG_TT
Cuts at: 1563 7506 7879 1563
Size: 5943 373 3063
Fragments arranged by size:
5943 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2949 3110 3626 3836 3917 4001 4427 4994
Size: 1317 161 516 210 81 84 426 567
Cuts at: 4994 6143 6408 6591 7442 8826 9094 1632
Size: 1149 265 183 851 1384 268 1917
Fragments arranged by size:
1917 1384 1317 1149 851 567 516 426
268 265 210 183 161 84 81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9182
AflIII A'CryG_T
Cuts at: 1203 5034 6166 8278 9255 1203
Size: 3831 1132 2112 977 1327
Fragments arranged by size:
3831 2112 1327 1132 977
AgeI A'CCGG_T
Cuts at: 8210 8210
Size: 9379
AhdI GACnn_n'nnGTC
Cuts at: 2655 3457 4938 8108 2655
Size: 802 1481 3170 3926
Fragments arranged by size:
3926 3170 1481 802
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3936 1184
Size: 2752 6627
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3834 3866 5021 5053 3834
Size: 32 1155 32 8160
Fragments arranged by size:
8160 1155 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2606 4972 7122 8694 2606
Size: 2366 2150 1572 3291
Fragments arranged by size:
3291 2366 2150 1572
ApaLI G'TGCA_C
Cuts at: 7436 8592 7436
Size: 1156 8223
ApoI r'AATT_y
Cuts at: 1493 3256 3719 4195 4304 4331 4496 4566 5048
Size: 1763 463 476 109 27 165 70 482
Cuts at: 5048 6688 6914 7066 9175 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1763 1697 1640 482 476 463 226
165 152 109 70 27
AseI AT'TA_AT
Cuts at: 160 1032 2889 6281 7933 160
Size: 872 1857 3392 1652 1606
Fragments arranged by size:
3392 1857 1652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9379
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2135 6704 1870
Size: 73 174 18 4569 4545
Fragments arranged by size:
4569 4545 174 73 18
AvrII C'CTAG_G
Cuts at: 3410 3410
Size: 9379
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2952 2985 2952
Size: 33 9346
BamHI G'GATC_C
Cuts at: 4128 6709 7076 4128
Size: 2581 367 6431
Fragments arranged by size:
6431 2581 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 3415 4757 5464 5778
Size: 325 217 627 462 1166 1342 707 314
Cuts at: 5778 6251 6382 6700 8155 618
Size: 473 131 318 1455 1842
Fragments arranged by size:
1842 1455 1342 1166 707 627 473 462
325 318 314 217 131
BanII G_rGCy'C
Cuts at: 1391 4405 4416 4735 6084 6698 1391
Size: 3014 11 319 1349 614 4072
Fragments arranged by size:
4072 3014 1349 614 319 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2217 928
Size: 971 318 8090
Fragments arranged by size:
8090 971 318
BbvCI CC'TCA_GC
Cuts at: 2570 4614 5058 2570
Size: 2044 444 6891
Fragments arranged by size:
6891 2044 444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4709 4743 5923 5957 7570 7604 1903
Size: 34 2772 34 1180 34 1613 34 3678
Fragments arranged by size:
3678 2772 1613 1180 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7271 8481 7271
Size: 1210 8169
BclI T'GATC_A
Cuts at: 1548 2470 1548
Size: 922 8457
BfrBI ATG'CAT
Cuts at: 4985 5379 4985
Size: 394 8985
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7990 136
Size: 107 122 71 631 605 6318 1525
Fragments arranged by size:
6318 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9374 9374
Size: 9379
BlpI GC'TnA_GC
Cuts at: 2592 6796 2592
Size: 4204 5175
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2613 3239 3676 4760 7440 8596 1790
Size: 378 445 626 437 1084 2680 1156 2573
Fragments arranged by size:
2680 2573 1156 1084 626 445 437 378
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4508 6299 7110
Size: 416 53 83 399 24 3091 1791 811
Cuts at: 7110 8068 442
Size: 958 1753
Fragments arranged by size:
3091 1791 1753 958 811 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3556 3945 4055 5499 6460 8039 1239
Size: 2317 389 110 1444 961 1579 2579
Fragments arranged by size:
2579 2317 1579 1444 961 389 110
Bpu10I CC'TnA_GC
Cuts at: 2570 4614 5058 2570
Size: 2044 444 6891
Fragments arranged by size:
6891 2044 444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3506 3961 4137 5268 5371 5590 5881 6620 7504
Size: 455 176 1131 103 219 291 739 884
Cuts at: 7504 8369 8667 3506
Size: 865 298 4218
Fragments arranged by size:
4218 1131 884 865 739 455 298 291
219 176 103
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2114 3055 3910 4515 8042 882
Size: 617 615 941 855 605 3527 2219
Fragments arranged by size:
3527 2219 941 855 617 615 605
BsaAI yAC'GTr
Cuts at: 493 1204 3677 7170 493
Size: 711 2473 3493 2702
Fragments arranged by size:
3493 2702 2473 711
BsaBI GATnn'nnATC
Cuts at: 2550 4701 9373 2550
Size: 2151 4672 2556
Fragments arranged by size:
4672 2556 2151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7186 7568 8203 275
Size: 53 83 186 936 5653 382 635 1451
Fragments arranged by size:
5653 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2110 3418 7812 8210 8484 8631 9042 2110
Size: 1308 4394 398 274 147 411 2447
Fragments arranged by size:
4394 2447 1308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 9154
Fragments arranged by size:
9154 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2025 2087 3797 3824 4245 4631 5633 5661
Size: 839 62 1710 27 421 386 1002 28
Cuts at: 5661 5806 6629 1186
Size: 145 823 3936
Fragments arranged by size:
3936 1710 1002 839 823 421 386 145
62 28 27
BseYI C'CCAG_C
Cuts at: 2261 3519 4752 6151 8582 9078 2261
Size: 1258 1233 1399 2431 496 2562
Fragments arranged by size:
2562 2431 1399 1258 1233 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2616 3194 3773 1163
Size: 573 880 578 579 6769
Fragments arranged by size:
6769 880 579 578 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3700 6432 6829 7590 8618 1540
Size: 275 186 1699 2732 397 761 1028 2301
Fragments arranged by size:
2732 2301 1699 1028 761 397 275 186
BsiHKAI G_wGCw'C
Cuts at: 2495 3529 3542 4416 6084 6504 7440 7525 8596
Size: 1034 13 874 1668 420 936 85 1071
Cuts at: 8596 2495
Size: 3278
Fragments arranged by size:
3278 1668 1071 1034 936 874 420 85
13
BsmI GAATG_Cn'
Cuts at: 2362 4278 5381 6890 6983 2362
Size: 1916 1103 1509 93 4758
Fragments arranged by size:
4758 1916 1509 1103 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 2495 2613 3239 3529 3542 3676
Size: 399 378 327 118 626 290 13 134
Cuts at: 3676 4405 4416 4735 4760 6084 6504 6698 7440
Size: 729 11 319 25 1324 420 194 742
Cuts at: 7440 7525 8596 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1324 1071 742 729 626 420 399
378 327 319 290 194 134 118 85
25 13 11
BspEI T'CCGG_A
Cuts at: 9042 9042
Size: 9379
BspHI T'CATG_A
Cuts at: 3025 7268 8998 3025
Size: 4243 1730 3406
Fragments arranged by size:
4243 3406 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2638 3052 3853 3928 4816 5323 5939 6734
Size: 1794 414 801 75 888 507 616 795
Cuts at: 6734 844
Size: 3489
Fragments arranged by size:
3489 1794 888 801 795 616 507 414
75
BsrBI CCG'CTC
Cuts at: 1394 1823 7266 1394
Size: 429 5443 3507
Fragments arranged by size:
5443 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2593 3113 4263 5017 5115 6198 7874
Size: 1183 1345 520 1150 754 98 1083 1676
Cuts at: 7874 8048 65
Size: 174 1396
Fragments arranged by size:
1676 1396 1345 1183 1150 1083 754 520
174 98
BsrFI r'CCGG_y
Cuts at: 1700 1810 4945 8023 8210 1700
Size: 110 3135 3078 187 2869
Fragments arranged by size:
3135 3078 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2485 5925 6099 96
Size: 2389 3440 174 3376
Fragments arranged by size:
3440 3376 2389 174
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9379
BssSI C'ACGA_G
Cuts at: 6399 7439 8451 6399
Size: 1040 1012 7327
Fragments arranged by size:
7327 1040 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9379
BstBI TT'CG_AA
Cuts at: 3840 6036 8183 3840
Size: 2196 2147 5036
Fragments arranged by size:
5036 2196 2147
BstEII G'GTnAC_C
Cuts at: 1184 5869 1184
Size: 4685 4694
BstXI CCAn_nnnn'nTGG
Cuts at: 6224 6224
Size: 9379
BstYI r'GATC_y
Cuts at: 1972 2439 2519 2551 3727 4081 4128 6709 7076
Size: 467 80 32 1176 354 47 2581 367
Cuts at: 7076 7461 7478 8930 9016 9028 9374 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2581 1977 1452 1176 467 385 367 354
346 86 80 47 32 17 12
Bsu36I CC'TnA_GG
Cuts at: 4791 4791
Size: 9379
BtgI C'CryG_G
Cuts at: 513 1065 2067 3822 5613 6418 513
Size: 552 1002 1755 1791 805 3474
Fragments arranged by size:
3474 1791 1755 1002 805 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4143 4936 6135 487
Size: 683 56 2917 793 1199 3731
Fragments arranged by size:
3731 2917 1199 793 683 56
BtsI GCAGTG_nn'
Cuts at: 2984 3799 4363 6592 6895 7689 7709 2984
Size: 815 564 2229 303 794 20 4654
Fragments arranged by size:
4654 2229 815 794 564 303 20
ClaI AT'CG_AT
Cuts at: 1554 4695 5554 1554
Size: 3141 859 5379
Fragments arranged by size:
5379 3141 859
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9344
DraI TTT'AAA
Cuts at: 2673 4170 6683 2673
Size: 1497 2513 5369
Fragments arranged by size:
5369 2513 1497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8386 809
Size: 507 7070 1802
Fragments arranged by size:
7070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3275 5383 5961
Size: 54 1425 50 275 18 1445 2108 578
Cuts at: 5961 6429 6826 7715 9344 8
Size: 468 397 889 1629 43
Fragments arranged by size:
2108 1629 1445 1425 889 578 468 397
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6429 6826 1537
Size: 275 4617 397 4090
Fragments arranged by size:
4617 4090 397 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3129 4071 4421 4616 6132 7309 1353
Size: 1776 942 350 195 1516 1177 3423
Fragments arranged by size:
3423 1776 1516 1177 942 350 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7966 8340 8486 387
Size: 1064 138 6377 374 146 1280
Fragments arranged by size:
6377 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4414 6082 4414
Size: 1668 7711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2949 3110 3556 3626 3836 3917 3945
Size: 393 1317 161 446 70 210 81 28
Cuts at: 3945 4001 4055 4427 4994 5499 6143 6408 6460
Size: 56 54 372 567 505 644 265 52
Cuts at: 6460 6591 7442 8039 8826 9094 1239
Size: 131 851 597 787 268 1524
Fragments arranged by size:
1524 1317 851 787 644 597 567 505
446 393 372 268 265 210 161 131
81 70 56 54 52 28
EcoNI CCTnn'n_nnAGG
Cuts at: 3667 5415 3667
Size: 1748 7631
EcoO109I rG'GnC_Cy
Cuts at: 3976 4477 5589 5729 3976
Size: 501 1112 140 7626
Fragments arranged by size:
7626 1112 501 140
EcoRI G'AATT_C
Cuts at: 4496 6688 7066 4496
Size: 2192 378 6809
Fragments arranged by size:
6809 2192 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4432 5890 4432
Size: 1458 7921
FspI TGC'GCA
Cuts at: 4863 7885 4863
Size: 3022 6357
HaeII r_GCGC'y
Cuts at: 1678 8526 1678
Size: 6848 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 2641 2673 3443
Size: 32 135 32 539 32 498 32 770
Cuts at: 3443 3458 3475 3490 4919 4924 4951 4956 8020
Size: 15 17 15 1429 5 27 5 3064
Cuts at: 8020 8052 8094 8126 1373
Size: 32 42 32 2626
Fragments arranged by size:
3064 2626 1429 770 539 498 135 42
32 32 32 32 32 32 27 17
15 15 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2292 2914 4807 5944 6723 6969
Size: 988 281 354 622 1893 1137 779 246
Cuts at: 6969 669
Size: 3079
Fragments arranged by size:
3079 1893 1137 988 779 622 354 281
246
HindIII A'AGCT_T
Cuts at: 748 2594 2705 3482 4250 4884 4959 5206 748
Size: 1846 111 777 768 634 75 247 4921
Fragments arranged by size:
4921 1846 777 768 634 247 111 75
HpaI GTT'AAC
Cuts at: 6969 6969
Size: 9379
KpnI G_GTAC'C
Cuts at: 2253 3419 5468 5782 6704 2253
Size: 1166 2049 314 922 4928
Fragments arranged by size:
4928 2049 1166 922 314
MfeI C'AATT_G
Cuts at: 5307 6978 5307
Size: 1671 7708
MluI A'CGCG_T
Cuts at: 9255 9255
Size: 9379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3061 3345 4389 8493 8677 1305
Size: 1756 284 1044 4104 184 2007
Fragments arranged by size:
4104 2007 1756 1044 284 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3277 5385 5963 10
Size: 54 1425 343 1445 2108 578 3426
Fragments arranged by size:
3426 2108 1445 1425 578 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4826 6107 6222 6423 6464 6495 7337
Size: 648 3660 1281 115 201 41 31 842
Cuts at: 7337 7696 7855 518
Size: 359 159 2042
Fragments arranged by size:
3660 2042 1281 842 648 359 201 159
115 41 31
MspA1I CmG'CkG
Cuts at: 1606 2018 3955 4652 4752 4972 6795 7472 8620
Size: 412 1937 697 100 220 1823 677 1148
Cuts at: 8620 8865 1606
Size: 245 2120
Fragments arranged by size:
2120 1937 1823 1148 697 677 412 245
220 100
NcoI C'CATG_G
Cuts at: 513 1065 2067 3822 6418 513
Size: 552 1002 1755 2596 3474
Fragments arranged by size:
3474 2596 1755 1002 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9379
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9379
NotI GC'GGCC_GC
Cuts at: 6826 6826
Size: 9379
NsiI A_TGCA'T
Cuts at: 4987 5381 4987
Size: 394 8985
NspI r_CATG'y
Cuts at: 1132 1229 2290 2822 4825 5038 6170 6737 8282
Size: 97 1061 532 2003 213 1132 567 1545
Cuts at: 8282 1132
Size: 2229
Fragments arranged by size:
2229 2003 1545 1132 1061 567 532 213
97
PasI CC'CwG_GG
Cuts at: 1348 6361 1348
Size: 5013 4366
PciI A'CATG_T
Cuts at: 5034 6166 8278 5034
Size: 1132 2112 6135
Fragments arranged by size:
6135 2112 1132
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9379
PfoI T'CCnGG_A
Cuts at: 2992 3162 4527 2992
Size: 170 1365 7844
Fragments arranged by size:
7844 1365 170
PmeI GTTT'AAAC
Cuts at: 6683 6683
Size: 9379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3921 3953 7448 7480 8961 8993 3921
Size: 32 3495 32 1481 32 4307
Fragments arranged by size:
4307 3495 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3976 4477 5589 5729 3976
Size: 501 1112 140 7626
Fragments arranged by size:
7626 1112 501 140
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9379
PsiI TTA'TAA
Cuts at: 6949 6949
Size: 9379
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3998 4030 3998
Size: 32 9347
PstI C_TGCA'G
Cuts at: 830 4026 4657 6019 6731 830
Size: 3196 631 1362 712 3478
Fragments arranged by size:
3478 3196 1362 712 631
PvuI CG_AT'CG
Cuts at: 2001 3700 2001
Size: 1699 7680
PvuII CAG'CTG
Cuts at: 3955 4652 4752 4972 3955
Size: 697 100 220 8362
Fragments arranged by size:
8362 697 220 100
SacI G_AGCT'C
Cuts at: 4416 6084 4416
Size: 1668 7711
SalI G'TCGA_C
Cuts at: 1936 2912 5942 6721 1936
Size: 976 3030 779 4594
Fragments arranged by size:
4594 3030 976 779
SapI GCTCTTCn'nnn_
Cuts at: 3129 4421 3129
Size: 1292 8087
SbfI CC_TGCA'GG
Cuts at: 6731 6731
Size: 9379
ScaI AGT'ACT
Cuts at: 4007 5096 6116 7627 9339 4007
Size: 1089 1020 1511 1712 4047
Fragments arranged by size:
4047 1712 1511 1089 1020
SfcI C'TryA_G
Cuts at: 826 1045 2581 2619 4022 4139 4287 4653 6015
Size: 219 1536 38 1403 117 148 366 1362
Cuts at: 6015 6727 7862 8543 8734 826
Size: 712 1135 681 191 1471
Fragments arranged by size:
1536 1471 1403 1362 1135 712 681 366
219 191 148 117 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9379
SmaI CCC'GGG
Cuts at: 1872 6706 1872
Size: 4834 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3485 3940 4152 5283 5350 5605
Size: 197 926 1542 455 212 1131 67 255
Cuts at: 5605 5860 6599 7483 8384 8646 820
Size: 255 739 884 901 262 1553
Fragments arranged by size:
1553 1542 1131 926 901 884 739 455
262 255 255 212 197 67
SnaBI TAC'GTA
Cuts at: 493 7170 493
Size: 6677 2702
SpeI A'CTAG_T
Cuts at: 152 4340 4843 152
Size: 4188 503 4688
Fragments arranged by size:
4688 4188 503
SphI G_CATG'C
Cuts at: 1132 2822 4825 6737 1132
Size: 1690 2003 1912 3774
Fragments arranged by size:
3774 2003 1912 1690
SspI AAT'ATT
Cuts at: 5 52 6033 7303 5
Size: 47 5981 1270 2081
Fragments arranged by size:
5981 2081 1270 47
StuI AGG'CCT
Cuts at: 1517 6484 1517
Size: 4967 4412
StyI C'CwwG_G
Cuts at: 513 1065 2067 2575 3410 3822 6418 6485 6818
Size: 552 1002 508 835 412 2596 67 333
Cuts at: 6818 513
Size: 3074
Fragments arranged by size:
3074 2596 1002 835 552 508 412 333
67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 2322 2504 7406 7423 7576 7761
Size: 1255 558 184 182 4902 17 153 185
Cuts at: 7761 8206 325
Size: 445 1498
Fragments arranged by size:
4902 1498 1255 558 445 185 184 182
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2485 3090 4005
Size: 274 80 33 51 1443 508 605 915
Cuts at: 4005 5094 5925 6099 6114 7625 9337 96
Size: 1089 831 174 15 1511 1712 138
Fragments arranged by size:
1712 1511 1443 1089 915 831 605 508
274 174 138 80 51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2382 2414 9339 9371 2382
Size: 32 6925 32 2390
Fragments arranged by size:
6925 2390 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2125 3564 1316
Size: 809 1439 7131
Fragments arranged by size:
7131 1439 809
XbaI T'CTAG_A
Cuts at: 2206 6715 2206
Size: 4509 4870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3732 5023 6338 6618 3732
Size: 1291 1315 280 6493
Fragments arranged by size:
6493 1315 1291 280
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9379
XmaI C'CCGG_G
Cuts at: 1870 6704 1870
Size: 4834 4545
XmnI GAAnn'nnTTC
Cuts at: 4253 5154 6032 7508 4253
Size: 901 878 1476 6124
Fragments arranged by size:
6124 1476 901 878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7187 276
Size: 53 83 186 936 5653 2468
Fragments arranged by size:
5653 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BbeI BplI BsiWI BsmBI BstZ17I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI