Restriction map for
pFN21AA0209
(Circular) (Six-base) MAPSORT of: pFN21AA0209.seq Check: 4267 from: 1 to: 10195
pFN21AA0209 10195 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4369 4484 4369
Size: 115 10080
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8005 278
Size: 53 83 186 936 6469 2468
Fragments arranged by size:
6469 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6363 7538 1937
Size: 4426 1175 4594
Fragments arranged by size:
4594 4426 1175
Acc65I G'GTAC_C
Cuts at: 5670 7516 5670
Size: 1846 8349
AclI AA'CG_TT
Cuts at: 1563 8322 8695 1563
Size: 6759 373 3063
Fragments arranged by size:
6759 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2447 2456 2852 3887 4175 4235 4619 5426
Size: 815 9 396 1035 288 60 384 807
Cuts at: 5426 6681 7184 7473 8258 9642 9910 1632
Size: 1255 503 289 785 1384 268 1917
Fragments arranged by size:
1917 1384 1255 1035 815 807 785 503
396 384 289 288 268 60 9
AflII C'TTAA_G
Cuts at: 820 1017 2924 820
Size: 197 1907 8091
Fragments arranged by size:
8091 1907 197
AflIII A'CryG_T
Cuts at: 1203 2123 4953 6462 9094 10071 1203
Size: 920 2830 1509 2632 977 1327
Fragments arranged by size:
2830 2632 1509 1327 977 920
AgeI A'CCGG_T
Cuts at: 9026 9026
Size: 10195
AhdI GACnn_n'nnGTC
Cuts at: 7480 8924 7480
Size: 1444 8751
AleI CACnn'nnGTG
Cuts at: 1166 3221 3818 1166
Size: 2055 597 7543
Fragments arranged by size:
7543 2055 597
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2962 3398 4134 1184
Size: 1778 436 736 7245
Fragments arranged by size:
7245 1778 736 436
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 10163
AlwNI CAG_nnn'CTG
Cuts at: 2959 4642 4750 5461 5723 5968 6284 7938 9510
Size: 1683 108 711 262 245 316 1654 1572
Cuts at: 9510 2959
Size: 3644
Fragments arranged by size:
3644 1683 1654 1572 711 316 262 245
108
ApaLI G'TGCA_C
Cuts at: 2947 6458 8252 9408 2947
Size: 3511 1794 1156 3734
Fragments arranged by size:
3734 3511 1794 1156
ApoI r'AATT_y
Cuts at: 1493 2256 2880 3901 4067 5629 5984 6958 7504
Size: 763 624 1021 166 1562 355 974 546
Cuts at: 7504 7730 7882 9991 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1562 1021 974 763 624 546
355 226 166 152
AseI AT'TA_AT
Cuts at: 160 1032 8749 160
Size: 872 7717 1606
Fragments arranged by size:
7717 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10195
AvaI C'yCGr_G
Cuts at: 1870 1943 3792 6184 7060 7520 1870
Size: 73 1849 2392 876 460 4545
Fragments arranged by size:
4545 2392 1849 876 460 73
BamHI G'GATC_C
Cuts at: 3516 6435 7525 7892 3516
Size: 2919 1090 367 5819
Fragments arranged by size:
5819 2919 1090 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5670 7516 8971 618
Size: 325 217 627 3883 1846 1455 1842
Fragments arranged by size:
3883 1846 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2077 2724 3761 5313 7514 1391
Size: 686 647 1037 1552 2201 4072
Fragments arranged by size:
4072 2201 1552 1037 686 647
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2514 2833 3510 4163 4993 7397 928
Size: 971 615 319 677 653 830 2404 3726
Fragments arranged by size:
3726 2404 971 830 677 653 615 319
BbvCI CC'TCA_GC
Cuts at: 7352 7352
Size: 10195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8386 8420 1903
Size: 34 6449 34 3678
Fragments arranged by size:
6449 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2138 2923 3949 4162 6035 8087 9297 2138
Size: 785 1026 213 1873 2052 1210 3036
Fragments arranged by size:
3036 2052 1873 1210 1026 785 213
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10195
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3096 8806 136
Size: 107 122 71 631 605 1424 5710 1525
Fragments arranged by size:
5710 1525 1424 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5937 10190 5937
Size: 4253 5942
BlpI GC'TnA_GC
Cuts at: 2718 3749 5307 5957 7612 2718
Size: 1031 1558 650 1655 5301
Fragments arranged by size:
5301 1655 1558 1031 650
Bme1580I G_kGCm'C
Cuts at: 1790 2951 6462 8256 9412 1790
Size: 1161 3511 1794 1156 2573
Fragments arranged by size:
3511 2573 1794 1161 1156
BmgBI CAC'GTC
Cuts at: 1318 2124 2451 1318
Size: 806 327 9062
Fragments arranged by size:
9062 806 327
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6139 7926 8884
Size: 416 53 83 399 24 4722 1787 958
Cuts at: 8884 442
Size: 1753
Fragments arranged by size:
4722 1787 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10195
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3451 5161 6421 3451
Size: 1710 1260 7225
Fragments arranged by size:
7225 1710 1260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 3745 3935 4112 4159 4481 4631 4664
Size: 1094 1412 190 177 47 322 150 33
Cuts at: 4664 4983 5156 5483 5570 6509 8855 1239
Size: 319 173 327 87 939 2346 2579
Fragments arranged by size:
2579 2346 1412 1094 939 327 322 319
190 177 173 150 87 47 33
Bpu10I CC'TnA_GC
Cuts at: 4691 7352 4691
Size: 2661 7534
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2501 3644 5741 5816 6695 7217 8320 9185 9483
Size: 1143 2097 75 879 522 1103 865 298
Cuts at: 9483 2501
Size: 3213
Fragments arranged by size:
3213 2097 1143 1103 879 865 522 298
75
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4868 4910 5843 6557 8858 882
Size: 617 3369 42 933 714 2301 2219
Fragments arranged by size:
3369 2301 2219 933 714 617 42
BsaAI yAC'GTr
Cuts at: 493 1204 6319 7986 493
Size: 711 5115 1667 2702
Fragments arranged by size:
5115 2702 1667 711
BsaBI GATnn'nnATC
Cuts at: 5936 6255 6758 6935 10189 5936
Size: 319 503 177 3254 5942
Fragments arranged by size:
5942 3254 503 319 177
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7028 7238 8002 8384
Size: 53 83 186 936 5495 210 764 382
Cuts at: 8384 9019 275
Size: 635 1451
Fragments arranged by size:
5495 1451 936 764 635 382 210 186
83 53
BsaWI w'CCGG_w
Cuts at: 4062 4764 5313 6804 8628 9026 9300 9447 9858
Size: 702 549 1491 1824 398 274 147 411
Cuts at: 9858 4062
Size: 4399
Fragments arranged by size:
4399 1824 1491 702 549 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2372 2402 3921 3951 4637
Size: 30 165 30 969 30 1519 30 686
Cuts at: 4637 4667 5645 5675 6180 6210 6569 6599 6836
Size: 30 978 30 505 30 359 30 237
Cuts at: 6836 6866 1178
Size: 30 4507
Fragments arranged by size:
4507 1519 978 969 686 505 359 237
165 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2641 2977 5669 1186
Size: 1455 336 2692 5712
Fragments arranged by size:
5712 2692 1455 336
BseYI C'CCAG_C
Cuts at: 2079 2391 3094 3235 3856 6749 6977 7288 9398
Size: 312 703 141 621 2893 228 311 2110
Cuts at: 9398 9894 2079
Size: 496 2380
Fragments arranged by size:
2893 2380 2110 703 621 496 312 311
228 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2375 2719 5596 1163
Size: 573 639 344 2877 5762
Fragments arranged by size:
5762 2877 639 573 344
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4253 7645 8406 9434 1540
Size: 275 186 2252 3392 761 1028 2301
Fragments arranged by size:
3392 2301 2252 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2724 2951 3761 3827 5313 5496 6462 7070 8256
Size: 227 810 66 1486 183 966 608 1186
Cuts at: 8256 8341 9412 2724
Size: 85 1071 3507
Fragments arranged by size:
3507 1486 1186 1071 966 810 608 227
183 85 66
BsmI GAATG_Cn'
Cuts at: 3991 4565 6208 6499 7706 7799 3991
Size: 574 1643 291 1207 93 6387
Fragments arranged by size:
6387 1643 1207 574 291 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2114 4309 5185 5831 2114
Size: 2195 876 646 6478
Fragments arranged by size:
6478 2195 876 646
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2724 2951 3761 3827 5313 5496
Size: 399 287 647 227 810 66 1486 183
Cuts at: 5496 6462 7070 7514 8256 8341 9412 1391
Size: 966 608 444 742 85 1071 2174
Fragments arranged by size:
2174 1486 1071 966 810 742 647 608
444 399 287 227 183 85 66
BspEI T'CCGG_A
Cuts at: 5313 9858 5313
Size: 4545 5650
BspHI T'CATG_A
Cuts at: 3981 6000 7191 8084 9814 3981
Size: 2019 1191 893 1730 4362
Fragments arranged by size:
4362 2019 1730 1191 893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4369 4484 5631 7550 844
Size: 3525 115 1147 1919 3489
Fragments arranged by size:
3525 3489 1919 1147 115
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 8082 1394
Size: 429 212 6047 3507
Fragments arranged by size:
6047 3507 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 2640 4053 4196 5502 8690 8864 65
Size: 1183 1392 1413 143 1306 3188 174 1396
Fragments arranged by size:
3188 1413 1396 1392 1306 1183 174 143
BsrFI r'CCGG_y
Cuts at: 1700 1810 5823 8839 9026 1700
Size: 110 4013 3016 187 2869
Fragments arranged by size:
4013 3016 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5661 5676 96
Size: 5565 15 4615
Fragments arranged by size:
5565 4615 15
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10195
BssSI C'ACGA_G
Cuts at: 5417 8255 9267 5417
Size: 2838 1012 6345
Fragments arranged by size:
6345 2838 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5452 5610 794
Size: 4658 158 5379
Fragments arranged by size:
5379 4658 158
BstBI TT'CG_AA
Cuts at: 8999 8999
Size: 10195
BstEII G'GTnAC_C
Cuts at: 1184 3145 4847 1184
Size: 1961 1702 6532
Fragments arranged by size:
6532 1961 1702
BstXI CCAn_nnnn'nTGG
Cuts at: 3223 3235 4984 5515 7492 3223
Size: 12 1749 531 1977 5926
Fragments arranged by size:
5926 1977 1749 531 12
BstYI r'GATC_y
Cuts at: 1972 2646 2848 2920 3516 5937 6435 6528 6732
Size: 674 202 72 596 2421 498 93 204
Cuts at: 6732 6759 7467 7525 7892 8277 8294 9746 9832
Size: 27 708 58 367 385 17 1452 86
Cuts at: 9832 9844 10190 1972
Size: 12 346 1977
Fragments arranged by size:
2421 1977 1452 708 674 596 498 385
367 346 204 202 93 86 72 58
27 17 12
Bsu36I CC'TnA_GG
Cuts at: 2805 5301 6011 6684 7077 2805
Size: 2496 710 673 393 5923
Fragments arranged by size:
5923 2496 710 673 393
BtgI C'CryG_G
Cuts at: 513 1065 2004 3219 4779 6094 6513 7485 513
Size: 552 939 1215 1560 1315 419 972 3223
Fragments arranged by size:
3223 1560 1315 1215 972 939 552 419
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2115 2797 487
Size: 683 56 889 682 7885
Fragments arranged by size:
7885 889 683 682 56
BtsI GCAGTG_nn'
Cuts at: 3772 5109 5780 7711 8505 8525 3772
Size: 1337 671 1931 794 20 5442
Fragments arranged by size:
5442 1931 1337 794 671 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10195
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3381 3416 529
Size: 35 2817 35 7308
Fragments arranged by size:
7308 2817 35 35
DraI TTT'AAA
Cuts at: 4549 7499 4549
Size: 2950 7245
DraIII CAC_nnn'GTG
Cuts at: 2044 3205 3727 4813 2044
Size: 1161 522 1086 7426
Fragments arranged by size:
7426 1161 1086 522
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3653 4019 6617 9202 809
Size: 507 2337 366 2598 2585 1802
Fragments arranged by size:
2598 2585 2337 1802 507 366
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2325 3225
Size: 54 1425 50 275 18 216 279 900
Cuts at: 3225 4974 6049 6516 7221 7437 7642 8531 10160
Size: 1749 1075 467 705 216 205 889 1629
Cuts at: 10160 8
Size: 43
Fragments arranged by size:
1749 1629 1425 1075 900 889 705 467
279 275 216 216 205 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 7642 1537
Size: 275 5830 4090
Fragments arranged by size:
5830 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2729 3977 5883 6109 6657 7097 8125 1353
Size: 1376 1248 1906 226 548 440 1028 3423
Fragments arranged by size:
3423 1906 1376 1248 1028 548 440 226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5933 8782 9156 9302 387
Size: 1064 138 4344 2849 374 146 1280
Fragments arranged by size:
4344 2849 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2722 3759 5311 2722
Size: 1037 1552 7606
Fragments arranged by size:
7606 1552 1037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2447 2456 2852 3745 3887 3935
Size: 393 701 114 9 396 893 142 48
Cuts at: 3935 4112 4159 4175 4235 4481 4619 4631 4664
Size: 177 47 16 60 246 138 12 33
Cuts at: 4664 4983 5156 5426 5483 5570 6509 6681 7184
Size: 319 173 270 57 87 939 172 503
Cuts at: 7184 7473 8258 8855 9642 9910 1239
Size: 289 785 597 787 268 1524
Fragments arranged by size:
1524 939 893 787 785 701 597 503
396 393 319 289 270 268 246 177
173 172 142 138 114 87 60 57
48 47 33 16 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 4376 7076 4376
Size: 2700 7495
EcoO109I rG'GnC_Cy
Cuts at: 2388 2967 4639 4936 5273 6715 2388
Size: 579 1672 297 337 1442 5868
Fragments arranged by size:
5868 1672 1442 579 337 297
EcoRI G'AATT_C
Cuts at: 6958 7504 7882 6958
Size: 546 378 9271
Fragments arranged by size:
9271 546 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 3058 4180 6670 2857
Size: 201 1122 2490 6382
Fragments arranged by size:
6382 2490 1122 201
FspI TGC'GCA
Cuts at: 6350 8701 6350
Size: 2351 7844
HaeII r_GCGC'y
Cuts at: 1678 7301 9342 1678
Size: 5623 2041 2531
Fragments arranged by size:
5623 2531 2041
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2356 2372 2388 2404 3505
Size: 32 135 32 784 16 16 16 1101
Cuts at: 3505 3537 3788 3820 4336 4368 7466 7498 8836
Size: 32 251 32 516 32 3098 32 1338
Cuts at: 8836 8868 8910 8942 1373
Size: 32 42 32 2626
Fragments arranged by size:
3098 2626 1338 1101 784 516 251 135
42 32 32 32 32 32 32 32
32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 7539 7785 669
Size: 988 281 5601 246 3079
Fragments arranged by size:
5601 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5231 748
Size: 4483 5712
HpaI GTT'AAC
Cuts at: 7785 7785
Size: 10195
KpnI G_GTAC'C
Cuts at: 5674 7520 5674
Size: 1846 8349
MfeI C'AATT_G
Cuts at: 7794 7794
Size: 10195
MluI A'CGCG_T
Cuts at: 10071 10071
Size: 10195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4129 5745 6019 6336 7137 7305 7393 9309
Size: 2824 1616 274 317 801 168 88 1916
Cuts at: 9309 9493 1305
Size: 184 2007
Fragments arranged by size:
2824 2007 1916 1616 801 317 274 184
168 88
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 2327 3227 4976 7439
Size: 54 1425 343 216 279 900 1749 2463
Cuts at: 7439 10
Size: 2766
Fragments arranged by size:
2766 2463 1749 1425 900 343 279 216
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2562 3155 3221 3356 3818 3983 4116
Size: 648 1396 593 66 135 462 165 133
Cuts at: 4116 4778 4982 8153 8512 8671 518
Size: 662 204 3171 359 159 2042
Fragments arranged by size:
3171 2042 1396 662 648 593 462 359
204 165 159 135 133 66
MspA1I CmG'CkG
Cuts at: 1606 2070 3068 4747 5077 5134 5458 6096 6136
Size: 464 998 1679 330 57 324 638 40
Cuts at: 6136 7148 7611 8288 9436 9681 1606
Size: 1012 463 677 1148 245 2120
Fragments arranged by size:
2120 1679 1148 1012 998 677 638 464
463 330 324 245 57 40
NcoI C'CATG_G
Cuts at: 513 1065 2004 4779 513
Size: 552 939 2775 5929
Fragments arranged by size:
5929 2775 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10195
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10195
NotI GC'GGCC_GC
Cuts at: 7642 7642
Size: 10195
NspI r_CATG'y
Cuts at: 1132 1229 3511 4630 4821 5455 6466 7220 7553
Size: 97 2282 1119 191 634 1011 754 333
Cuts at: 7553 9098 1132
Size: 1545 2229
Fragments arranged by size:
2282 2229 1545 1119 1011 754 634 333
191 97
PasI CC'CwG_GG
Cuts at: 1348 7319 1348
Size: 5971 4224
PciI A'CATG_T
Cuts at: 6462 9094 6462
Size: 2632 7563
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4642 1108
Size: 3534 6661
PfoI T'CCnGG_A
Cuts at: 2642 3951 4641 5547 7175 2642
Size: 1309 690 906 1628 5662
Fragments arranged by size:
5662 1628 1309 906 690
PmeI GTTT'AAAC
Cuts at: 7499 7499
Size: 10195
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6161 6193 8264 8296 9777 9809 6161
Size: 32 2071 32 1481 32 6547
Fragments arranged by size:
6547 2071 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2388 4639 4936 5273 6715 2388
Size: 2251 297 337 1442 5868
Fragments arranged by size:
5868 2251 1442 337 297
PshAI GACnn'nnGTC
Cuts at: 1613 3544 6931 1613
Size: 1931 3387 4877
Fragments arranged by size:
4877 3387 1931
PsiI TTA'TAA
Cuts at: 6296 7765 6296
Size: 1469 8726
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 10163
PstI C_TGCA'G
Cuts at: 830 2094 2254 5133 5491 6971 7153 7547 830
Size: 1264 160 2879 358 1480 182 394 3478
Fragments arranged by size:
3478 2879 1480 1264 394 358 182 160
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10195
PvuII CAG'CTG
Cuts at: 3068 4747 5077 5134 5458 6136 7148 3068
Size: 1679 330 57 324 678 1012 6115
Fragments arranged by size:
6115 1679 1012 678 330 324 57
SacI G_AGCT'C
Cuts at: 2724 3761 5313 2724
Size: 1037 1552 7606
Fragments arranged by size:
7606 1552 1037
SacII CC_GC'GG
Cuts at: 6097 6097
Size: 10195
SalI G'TCGA_C
Cuts at: 1936 7537 1936
Size: 5601 4594
SapI GCTCTTCn'nnn_
Cuts at: 2729 2729
Size: 10195
SbfI CC_TGCA'GG
Cuts at: 7547 7547
Size: 10195
ScaI AGT'ACT
Cuts at: 4655 8443 10155 4655
Size: 3788 1712 4695
Fragments arranged by size:
4695 3788 1712
SexAI A'CCwGG_T
Cuts at: 3201 4635 5532 7491 3201
Size: 1434 897 1959 5905
Fragments arranged by size:
5905 1959 1434 897
SfcI C'TryA_G
Cuts at: 826 1045 2090 2250 3893 5129 5487 6967 7149
Size: 219 1045 160 1643 1236 358 1480 182
Cuts at: 7149 7543 8678 9359 9550 826
Size: 394 1135 681 191 1471
Fragments arranged by size:
1643 1480 1471 1236 1135 1045 681 394
358 219 191 182 160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10195
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10195
SmaI CCC'GGG
Cuts at: 1872 3794 6186 7062 7522 1872
Size: 1922 2392 876 460 4545
Fragments arranged by size:
4545 2392 1922 876 460
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2480 2924 3659 5756 5831 6710
Size: 197 926 537 444 735 2097 75 879
Cuts at: 6710 7232 8299 9200 9462 820
Size: 522 1067 901 262 1553
Fragments arranged by size:
2097 1553 1067 926 901 879 735 537
522 444 262 197 75
SnaBI TAC'GTA
Cuts at: 493 7986 493
Size: 7493 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10195
SphI G_CATG'C
Cuts at: 1132 7220 7553 1132
Size: 6088 333 3774
Fragments arranged by size:
6088 3774 333
SspI AAT'ATT
Cuts at: 5 52 8119 5
Size: 47 8067 2081
Fragments arranged by size:
8067 2081 47
StuI AGG'CCT
Cuts at: 1517 3138 5544 6650 1517
Size: 1621 2406 1106 5062
Fragments arranged by size:
5062 2406 1621 1106
StyI C'CwwG_G
Cuts at: 513 1065 2004 2062 2415 3114 4125 4779 7634
Size: 552 939 58 353 699 1011 654 2855
Cuts at: 7634 513
Size: 3074
Fragments arranged by size:
3074 2855 1011 939 699 654 552 353
58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2678 3133 3155 3534 4239 4835 5132
Size: 1255 1098 455 22 379 705 596 297
Cuts at: 5132 5511 7036 8222 8239 8392 8577 9022 325
Size: 379 1525 1186 17 153 185 445 1498
Fragments arranged by size:
1525 1498 1255 1186 1098 705 596 455
445 379 379 297 185 153 22 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3315 3444 4510
Size: 274 80 33 51 1443 1338 129 1066
Cuts at: 4510 4653 5448 5661 5676 8441 10153 96
Size: 143 795 213 15 2765 1712 138
Fragments arranged by size:
2765 1712 1443 1338 1066 795 274 213
143 138 129 80 51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3921 3953 4938 4970 5759 5791 6180 6212 6466
Size: 32 985 32 789 32 389 32 254
Cuts at: 6466 6498 10155 10187 3921
Size: 32 3657 32 3929
Fragments arranged by size:
3929 3657 985 789 389 254 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10195
XbaI T'CTAG_A
Cuts at: 7531 7531
Size: 10195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3157 3814 6943 7291 7376 3157
Size: 657 3129 348 85 5976
Fragments arranged by size:
5976 3129 657 348 85
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10195
XmaI C'CCGG_G
Cuts at: 1870 3792 6184 7060 7520 1870
Size: 1922 2392 876 460 4545
Fragments arranged by size:
4545 2392 1922 876 460
XmnI GAAnn'nnTTC
Cuts at: 3077 6650 8324 3077
Size: 3573 1674 4948
Fragments arranged by size:
4948 3573 1674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8003 276
Size: 53 83 186 936 6469 2468
Fragments arranged by size:
6469 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI RsrII SanDI
SfiI SfoI SrfI SwaI