Restriction map for
pFN21AA0203
(Circular) (Six-base) MAPSORT of: pFN21AA0203.seq Check: 5299 from: 1 to: 9478
pFN21AA0203 9478 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2266 2266
Size: 9478
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7288 278
Size: 53 83 186 936 5752 2468
Fragments arranged by size:
5752 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4327 4438 5431 6821 1937
Size: 2390 111 993 1390 4594
Fragments arranged by size:
4594 2390 1390 993 111
Acc65I G'GTAC_C
Cuts at: 6799 6799
Size: 9478
AclI AA'CG_TT
Cuts at: 1563 7605 7978 1563
Size: 6042 373 3063
Fragments arranged by size:
6042 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3380 4417 4680 4877 4928 4973 5873 6141
Size: 1748 1037 263 197 51 45 900 268
Cuts at: 6141 6384 6447 6618 7541 8925 9193 1632
Size: 243 63 171 923 1384 268 1917
Fragments arranged by size:
1917 1748 1384 1037 923 900 268 268
263 243 197 171 63 51 45
AflII C'TTAA_G
Cuts at: 820 1017 2087 820
Size: 197 1070 8211
Fragments arranged by size:
8211 1070 197
AflIII A'CryG_T
Cuts at: 1203 2794 3349 8377 9354 1203
Size: 1591 555 5028 977 1327
Fragments arranged by size:
5028 1591 1327 977 555
AgeI A'CCGG_T
Cuts at: 8309 8309
Size: 9478
AhdI GACnn_n'nnGTC
Cuts at: 8207 8207
Size: 9478
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9478
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3858 1184
Size: 2674 6804
AlwNI CAG_nnn'CTG
Cuts at: 2137 2819 3757 5216 7221 8793 2137
Size: 682 938 1459 2005 1572 2822
Fragments arranged by size:
2822 2005 1572 1459 938 682
ApaLI G'TGCA_C
Cuts at: 6243 7535 8691 6243
Size: 1292 1156 7030
Fragments arranged by size:
7030 1292 1156
ApoI r'AATT_y
Cuts at: 1493 2488 4234 4507 4739 5229 5299 5747 6787
Size: 995 1746 273 232 490 70 448 1040
Cuts at: 6787 7013 7165 9274 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1746 1697 1040 995 490 448 273
232 226 152 70
AseI AT'TA_AT
Cuts at: 160 1032 5079 8032 160
Size: 872 4047 2953 1606
Fragments arranged by size:
4047 2953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9478
AvaI C'yCGr_G
Cuts at: 1870 1943 6803 1870
Size: 73 4860 4545
Fragments arranged by size:
4860 4545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4332 4365 4332
Size: 33 9445
BamHI G'GATC_C
Cuts at: 4036 6808 7175 4036
Size: 2772 367 6339
Fragments arranged by size:
6339 2772 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6799 8254 618
Size: 325 217 627 5012 1455 1842
Fragments arranged by size:
5012 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5254 6133 6797 1391
Size: 3863 879 664 4072
Fragments arranged by size:
4072 3863 879 664
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2559 2665 2691 4461 4890 6453 6655
Size: 971 660 106 26 1770 429 1563 202
Cuts at: 6655 928
Size: 3751
Fragments arranged by size:
3751 1770 1563 971 660 429 202 106
26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5945 5979 7669 7703 1903
Size: 34 4008 34 1690 34 3678
Fragments arranged by size:
4008 3678 1690 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2197 4333 7370 8580 2197
Size: 2136 3037 1210 3095
Fragments arranged by size:
3095 3037 2136 1210
BclI T'GATC_A
Cuts at: 1548 3391 1548
Size: 1843 7635
BfrBI ATG'CAT
Cuts at: 2160 3221 2160
Size: 1061 8417
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6701 8089 136
Size: 107 122 71 631 605 5029 1388 1525
Fragments arranged by size:
5029 1525 1388 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3904 9473 3904
Size: 5569 3909
BlpI GC'TnA_GC
Cuts at: 3496 5368 6895 3496
Size: 1872 1527 6079
Fragments arranged by size:
6079 1872 1527
Bme1580I G_kGCm'C
Cuts at: 1790 6247 7539 8695 1790
Size: 4457 1292 1156 2573
Fragments arranged by size:
4457 2573 1292 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9478
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7209 8167 442
Size: 416 53 83 399 24 5792 958 1753
Fragments arranged by size:
5792 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3138 1056
Size: 2082 7396
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4032 4032
Size: 9478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2495 4062 8138 1239
Size: 1256 1567 4076 2579
Fragments arranged by size:
4076 2579 1567 1256
Bpu10I CC'TnA_GC
Cuts at: 3174 3174
Size: 9478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4730 5132 5660 5825 6161 7603 8468 8766 4730
Size: 402 528 165 336 1442 865 298 5442
Fragments arranged by size:
5442 1442 865 528 402 336 298 165
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5258 8141 882
Size: 617 3759 2883 2219
Fragments arranged by size:
3759 2883 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 7269 493
Size: 711 6065 2702
Fragments arranged by size:
6065 2702 711
BsaBI GATnn'nnATC
Cuts at: 2208 2433 2901 9472 2208
Size: 225 468 6571 2214
Fragments arranged by size:
6571 2214 468 225
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7285 7667 8302 275
Size: 53 83 186 936 5752 382 635 1451
Fragments arranged by size:
5752 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7911 8309 8583 8730 9141 7911
Size: 398 274 147 411 8248
Fragments arranged by size:
8248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4686 4716 6237 6267 6507
Size: 30 165 30 3283 30 1521 30 240
Cuts at: 6507 6537 1178
Size: 30 4119
Fragments arranged by size:
4119 3283 1521 240 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2166 4005 4727 5862 1186
Size: 980 1839 722 1135 4802
Fragments arranged by size:
4802 1839 1135 980 722
BseYI C'CCAG_C
Cuts at: 2195 2451 5253 8681 9177 2195
Size: 256 2802 3428 496 2496
Fragments arranged by size:
3428 2802 2496 496 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8905
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6928 7689 8717 1540
Size: 275 186 4927 761 1028 2301
Fragments arranged by size:
4927 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2730 5572 6133 6247 7539 7624 8695 2730
Size: 2842 561 114 1292 85 1071 3513
Fragments arranged by size:
3513 2842 1292 1071 561 114 85
BsmI GAATG_Cn'
Cuts at: 2162 3047 4338 6478 6989 7082 2162
Size: 885 1291 2140 511 93 4558
Fragments arranged by size:
4558 2140 1291 885 511 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2696 4248 2696
Size: 1552 7926
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2730 5254 5572 6133 6247 6797 7539
Size: 399 940 2524 318 561 114 550 742
Cuts at: 7539 7624 8695 1391
Size: 85 1071 2174
Fragments arranged by size:
2524 2174 1071 940 742 561 550 399
318 114 85
BspEI T'CCGG_A
Cuts at: 9141 9141
Size: 9478
BspHI T'CATG_A
Cuts at: 7367 9097 7367
Size: 1730 7748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2266 6619 6833 844
Size: 1422 4353 214 3489
Fragments arranged by size:
4353 3489 1422 214
BsrBI CCG'CTC
Cuts at: 1394 1823 7365 1394
Size: 429 5542 3507
Fragments arranged by size:
5542 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6321 6398 7973 8147 65
Size: 1183 5073 77 1575 174 1396
Fragments arranged by size:
5073 1575 1396 1183 174 77
BsrFI r'CCGG_y
Cuts at: 1700 1810 8122 8309 1700
Size: 110 6312 187 2869
Fragments arranged by size:
6312 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2422 4480 4520 6337 96
Size: 2326 2058 40 1817 3237
Fragments arranged by size:
3237 2326 2058 1817 40
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9478
BssSI C'ACGA_G
Cuts at: 4866 5170 7538 8550 4866
Size: 304 2368 1012 5794
Fragments arranged by size:
5794 2368 1012 304
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9478
BstBI TT'CG_AA
Cuts at: 5965 8282 5965
Size: 2317 7161
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9478
BstXI CCAn_nnnn'nTGG
Cuts at: 2722 2788 2722
Size: 66 9412
BstYI r'GATC_y
Cuts at: 1972 2711 3904 4036 6272 6619 6808 7175 7560
Size: 739 1193 132 2236 347 189 367 385
Cuts at: 7560 7577 9029 9115 9127 9473 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2236 1977 1452 1193 739 385 367 347
346 189 132 86 17 12
BstZ17I GTA'TAC
Cuts at: 4439 4439
Size: 9478
Bsu36I CC'TnA_GG
Cuts at: 3794 3794
Size: 9478
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 8926
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8739
Fragments arranged by size:
8739 683 56
BtsI GCAGTG_nn'
Cuts at: 3318 6994 7788 7808 3318
Size: 3676 794 20 4988
Fragments arranged by size:
4988 3676 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9478
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9443
DraI TTT'AAA
Cuts at: 4614 5752 6718 6782 4614
Size: 1138 966 64 7310
Fragments arranged by size:
7310 1138 966 64
DraIII CAC_nnn'GTG
Cuts at: 2134 2134
Size: 9478
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8485 809
Size: 507 7169 1802
Fragments arranged by size:
7169 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2466 2601 3142
Size: 54 1425 50 275 18 636 135 541
Cuts at: 3142 6925 7814 9443 8
Size: 3783 889 1629 43
Fragments arranged by size:
3783 1629 1425 889 636 541 275 135
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6925 1537
Size: 275 5113 4090
Fragments arranged by size:
5113 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2170 3191 4656 5283 5604 5919 6295 7408
Size: 817 1021 1465 627 321 315 376 1113
Cuts at: 7408 1353
Size: 3423
Fragments arranged by size:
3423 1465 1113 1021 817 627 376 321
315
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3413 8065 8439 8585 387
Size: 1064 138 1824 4652 374 146 1280
Fragments arranged by size:
4652 1824 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6131 6131
Size: 9478
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2495 3380 4062 4417 4680 4877 4928
Size: 393 863 885 682 355 263 197 51
Cuts at: 4928 4973 5873 6141 6384 6447 6618 7541 8138
Size: 45 900 268 243 63 171 923 597
Cuts at: 8138 8925 9193 1239
Size: 787 268 1524
Fragments arranged by size:
1524 923 900 885 863 787 682 597
393 355 268 268 263 243 197 171
63 51 45
EcoNI CCTnn'n_nnAGG
Cuts at: 4712 4712
Size: 9478
EcoRI G'AATT_C
Cuts at: 6787 7165 6787
Size: 378 9100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2336 4652 5284 5903 2336
Size: 2316 632 619 5911
Fragments arranged by size:
5911 2316 632 619
FspI TGC'GCA
Cuts at: 6182 7984 6182
Size: 1802 7676
HaeII r_GCGC'y
Cuts at: 1678 8625 1678
Size: 6947 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2954 2986 3872 3904 4187
Size: 32 135 32 1382 32 886 32 283
Cuts at: 4187 4219 8119 8151 8193 8225 1373
Size: 32 3900 32 42 32 2626
Fragments arranged by size:
3900 2626 1382 886 283 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2029 2769 6822 7068 669
Size: 988 281 91 740 4053 246 3079
Fragments arranged by size:
4053 3079 988 740 281 246 91
HindIII A'AGCT_T
Cuts at: 748 3414 5702 5860 5885 6155 748
Size: 2666 2288 158 25 270 4071
Fragments arranged by size:
4071 2666 2288 270 158 25
HpaI GTT'AAC
Cuts at: 2029 2769 7068 2029
Size: 740 4299 4439
Fragments arranged by size:
4439 4299 740
KpnI G_GTAC'C
Cuts at: 6803 6803
Size: 9478
MfeI C'AATT_G
Cuts at: 5394 6404 7077 5394
Size: 1010 673 7795
Fragments arranged by size:
7795 1010 673
MluI A'CGCG_T
Cuts at: 9354 9354
Size: 9478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2598 4377 8592 8776 1305
Size: 1293 1779 4215 184 2007
Fragments arranged by size:
4215 2007 1779 1293 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2468 2603 10
Size: 54 1425 343 636 135 6885
Fragments arranged by size:
6885 1425 636 343 135 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3834 4516 7436 7795 7954 518
Size: 648 2668 682 2920 359 159 2042
Fragments arranged by size:
2920 2668 2042 682 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 6278 6376 6894 7571 8719 8964 1606
Size: 4672 98 518 677 1148 245 2120
Fragments arranged by size:
4672 2120 1148 677 518 245 98
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8926
NdeI CA'TA_TG
Cuts at: 387 6208 6354 387
Size: 5821 146 3511
Fragments arranged by size:
5821 3511 146
NheI G'CTAG_C
Cuts at: 1052 3134 1052
Size: 2082 7396
NotI GC'GGCC_GC
Cuts at: 6925 6925
Size: 9478
NsiI A_TGCA'T
Cuts at: 2162 3223 2162
Size: 1061 8417
NspI r_CATG'y
Cuts at: 1132 1229 2096 2798 3353 3839 6460 6836 8381
Size: 97 867 702 555 486 2621 376 1545
Cuts at: 8381 1132
Size: 2229
Fragments arranged by size:
2621 2229 1545 867 702 555 486 376
97
PasI CC'CwG_GG
Cuts at: 1348 6654 1348
Size: 5306 4172
PciI A'CATG_T
Cuts at: 2794 3349 8377 2794
Size: 555 5028 3895
Fragments arranged by size:
5028 3895 555
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2137 1108
Size: 1029 8449
PfoI T'CCnGG_A
Cuts at: 5112 5112
Size: 9478
PmeI GTTT'AAAC
Cuts at: 6782 6782
Size: 9478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2712 2744 7547 7579 9060 9092 2712
Size: 32 4803 32 1481 32 3098
Fragments arranged by size:
4803 3098 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 6445 1613
Size: 4832 4646
PsiI TTA'TAA
Cuts at: 7048 7048
Size: 9478
PstI C_TGCA'G
Cuts at: 830 2170 2335 2544 2590 4054 4785 5544 6830
Size: 1340 165 209 46 1464 731 759 1286
Cuts at: 6830 830
Size: 3478
Fragments arranged by size:
3478 1464 1340 1286 759 731 209 165
46
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9478
SacI G_AGCT'C
Cuts at: 6133 6133
Size: 9478
SalI G'TCGA_C
Cuts at: 1936 6820 1936
Size: 4884 4594
SapI GCTCTTCn'nnn_
Cuts at: 5919 5919
Size: 9478
SbfI CC_TGCA'GG
Cuts at: 6830 6830
Size: 9478
ScaI AGT'ACT
Cuts at: 2857 7726 9438 2857
Size: 4869 1712 2897
Fragments arranged by size:
4869 2897 1712
SexAI A'CCwGG_T
Cuts at: 2130 2130
Size: 9478
SfcI C'TryA_G
Cuts at: 826 1045 2166 2188 2331 2540 2586 3541 4050
Size: 219 1121 22 143 209 46 955 509
Cuts at: 4050 4781 5540 6216 6826 7961 8642 8833 826
Size: 731 759 676 610 1135 681 191 1471
Fragments arranged by size:
1471 1135 1121 955 759 731 681 676
610 509 219 209 191 143 46 22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9478
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9478
SmaI CCC'GGG
Cuts at: 1872 6805 1872
Size: 4933 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2087 4709 5147 5639 5804 6140
Size: 197 926 144 2622 438 492 165 336
Cuts at: 6140 7582 8483 8745 820
Size: 1442 901 262 1553
Fragments arranged by size:
2622 1553 1442 926 901 492 438 336
262 197 165 144
SnaBI TAC'GTA
Cuts at: 493 7269 493
Size: 6776 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9478
SphI G_CATG'C
Cuts at: 1132 2096 6836 1132
Size: 964 4740 3774
Fragments arranged by size:
4740 3774 964
SspI AAT'ATT
Cuts at: 5 52 6349 7402 5
Size: 47 6297 1053 2081
Fragments arranged by size:
6297 2081 1053 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9478
StyI C'CwwG_G
Cuts at: 513 1065 2446 3014 3965 4950 6917 513
Size: 552 1381 568 951 985 1967 3074
Fragments arranged by size:
3074 1967 1381 985 951 568 552
SwaI ATTT'AAAT
Cuts at: 5752 5752
Size: 9478
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7505 7522 7675 7860 8305 325
Size: 1255 5925 17 153 185 445 1498
Fragments arranged by size:
5925 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2422 2855 3473
Size: 274 80 33 51 1443 445 433 618
Cuts at: 3473 3489 3692 3838 3977 4480 4520 6071 6337
Size: 16 203 146 139 503 40 1551 266
Cuts at: 6337 7724 9436 96
Size: 1387 1712 138
Fragments arranged by size:
1712 1551 1443 1387 618 503 445 433
274 266 203 146 139 138 80 51
40 33 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6237 6269 9438 9470 6237
Size: 32 3169 32 6245
Fragments arranged by size:
6245 3169 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9478
XbaI T'CTAG_A
Cuts at: 5911 6644 6814 5911
Size: 733 170 8575
Fragments arranged by size:
8575 733 170
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9478
XmaI C'CCGG_G
Cuts at: 1870 6803 1870
Size: 4933 4545
XmnI GAAnn'nnTTC
Cuts at: 2436 4116 5727 5886 6348 7607 2436
Size: 1680 1611 159 462 1259 4307
Fragments arranged by size:
4307 1680 1611 1259 462 159
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7286 276
Size: 53 83 186 936 5752 2468
Fragments arranged by size:
5752 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BsiWI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SfiI SfoI SrfI XcmI