Restriction map for
pFN21AA0188
(Circular) (Six-base) MAPSORT of: pFN21AA0188.seq Check: 2183 from: 1 to: 7375
pFN21AA0188 7375 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5185 278
Size: 53 83 186 936 3649 2468
Fragments arranged by size:
3649 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3195 4542 4718 1937
Size: 1258 1347 176 4594
Fragments arranged by size:
4594 1347 1258 176
Acc65I G'GTAC_C
Cuts at: 4696 4696
Size: 7375
AclI AA'CG_TT
Cuts at: 1563 5502 5875 1563
Size: 3939 373 3063
Fragments arranged by size:
3939 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 2931 3246 4563 4588 5438 6822 7090
Size: 893 406 315 1317 25 850 1384 268
Cuts at: 7090 1632
Size: 1917
Fragments arranged by size:
1917 1384 1317 893 850 406 315 268
25
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7178
AflIII A'CryG_T
Cuts at: 1203 2544 6274 7251 1203
Size: 1341 3730 977 1327
Fragments arranged by size:
3730 1341 1327 977
AgeI A'CCGG_T
Cuts at: 6206 6206
Size: 7375
AhdI GACnn_n'nnGTC
Cuts at: 6104 6104
Size: 7375
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7375
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4259 1184
Size: 3075 4300
AlwNI CAG_nnn'CTG
Cuts at: 5118 6690 5118
Size: 1572 5803
ApaI G_GGCC'C
Cuts at: 4257 4257
Size: 7375
ApaLI G'TGCA_C
Cuts at: 5432 6588 5432
Size: 1156 6219
ApoI r'AATT_y
Cuts at: 1493 2904 4157 4684 4910 5062 7171 1493
Size: 1411 1253 527 226 152 2109 1697
Fragments arranged by size:
2109 1697 1411 1253 527 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5929 160
Size: 872 4897 1606
Fragments arranged by size:
4897 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7375
AvaI C'yCGr_G
Cuts at: 1870 1943 2184 3337 4700 1870
Size: 73 241 1153 1363 4545
Fragments arranged by size:
4545 1363 1153 241 73
BamHI G'GATC_C
Cuts at: 3220 4705 5072 3220
Size: 1485 367 5523
Fragments arranged by size:
5523 1485 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3992 4696 6151 618
Size: 325 217 627 2205 704 1455 1842
Fragments arranged by size:
2205 1842 1455 704 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2058 2375 2567 3096 3301 4257 4694 1391
Size: 667 317 192 529 205 956 437 4072
Fragments arranged by size:
4072 956 667 529 437 317 205 192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2847 3048 3887 928
Size: 971 948 201 839 4416
Fragments arranged by size:
4416 971 948 839 201
BbvCI CC'TCA_GC
Cuts at: 3377 3419 4669 3377
Size: 42 1250 6083
Fragments arranged by size:
6083 1250 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5566 5600 1903
Size: 34 3629 34 3678
Fragments arranged by size:
3678 3629 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4591 5267 6477 4591
Size: 676 1210 5489
Fragments arranged by size:
5489 1210 676
BclI T'GATC_A
Cuts at: 1548 2287 1548
Size: 739 6636
BfrBI ATG'CAT
Cuts at: 3449 3449
Size: 7375
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 5986 136
Size: 107 122 71 631 605 2580 1734 1525
Fragments arranged by size:
2580 1734 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7370 7370
Size: 7375
BlpI GC'TnA_GC
Cuts at: 3767 4122 4792 3767
Size: 355 670 6350
Fragments arranged by size:
6350 670 355
Bme1580I G_kGCm'C
Cuts at: 1790 2992 3995 4238 4257 5436 6592 1790
Size: 1202 1003 243 19 1179 1156 2573
Fragments arranged by size:
2573 1202 1179 1156 1003 243 19
BmgBI CAC'GTC
Cuts at: 1318 2152 3943 1318
Size: 834 1791 4750
Fragments arranged by size:
4750 1791 834
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2718 4021 4228
Size: 416 53 83 399 24 1301 1303 207
Cuts at: 4228 5106 6064 442
Size: 878 958 1753
Fragments arranged by size:
1753 1303 1301 958 878 416 399 207
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7375
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2581 3382 2581
Size: 801 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2609 3068 3476 3787 6035 1239
Size: 1370 459 408 311 2248 2579
Fragments arranged by size:
2579 2248 1370 459 408 311
Bpu10I CC'TnA_GC
Cuts at: 3377 3419 4669 3377
Size: 42 1250 6083
Fragments arranged by size:
6083 1250 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5500 6365 6663 5500
Size: 865 298 6212
Fragments arranged by size:
6212 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3044 6038 882
Size: 617 1545 2994 2219
Fragments arranged by size:
2994 2219 1545 617
BsaAI yAC'GTr
Cuts at: 493 1204 2014 3978 5166 493
Size: 711 810 1964 1188 2702
Fragments arranged by size:
2702 1964 1188 810 711
BsaBI GATnn'nnATC
Cuts at: 2286 7369 2286
Size: 5083 2292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2438 3192 5182 5564
Size: 53 83 186 936 905 754 1990 382
Cuts at: 5564 6199 275
Size: 635 1451
Fragments arranged by size:
1990 1451 936 905 754 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2732 3266 3746 5808 6206 6480 6627 7038 2732
Size: 534 480 2062 398 274 147 411 3069
Fragments arranged by size:
3069 2062 534 480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3127 3157 3555 3585 4470
Size: 30 165 30 1724 30 398 30 885
Cuts at: 4470 4500 1178
Size: 30 4053
Fragments arranged by size:
4053 1724 885 398 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2494 3104 3560 3827 1186
Size: 1308 610 456 267 4734
Fragments arranged by size:
4734 1308 610 456 267
BseYI C'CCAG_C
Cuts at: 2402 2429 4271 6578 7074 2402
Size: 27 1842 2307 496 2703
Fragments arranged by size:
2703 2307 1842 496 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4199 4277 1163
Size: 573 2463 78 4261
Fragments arranged by size:
4261 2463 573 78
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4825 5586 6614 1540
Size: 275 186 2824 761 1028 2301
Fragments arranged by size:
2824 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2058 2567 3096 3365 5436 5521 6592 2058
Size: 509 529 269 2071 85 1071 2841
Fragments arranged by size:
2841 2071 1071 529 509 269 85
BsmI GAATG_Cn'
Cuts at: 2360 3449 4886 4979 2360
Size: 1089 1437 93 4756
Fragments arranged by size:
4756 1437 1089 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2429 2429
Size: 7375
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2375 2567 2992 3096 3301 3365
Size: 399 268 317 192 425 104 205 64
Cuts at: 3365 3995 4238 4257 4694 5436 5521 6592 1391
Size: 630 243 19 437 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 630 437 425 399 317
268 243 205 192 104 85 64 19
BspEI T'CCGG_A
Cuts at: 3266 7038 3266
Size: 3772 3603
BspHI T'CATG_A
Cuts at: 3627 5264 6994 3627
Size: 1637 1730 4008
Fragments arranged by size:
4008 1730 1637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4221 4730 844
Size: 3377 509 3489
Fragments arranged by size:
3489 3377 509
BsrBI CCG'CTC
Cuts at: 1394 1823 5262 1394
Size: 429 3439 3507
Fragments arranged by size:
3507 3439 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3585 5870 6044 65
Size: 1183 2337 2285 174 1396
Fragments arranged by size:
2337 2285 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 6019 6206 1700
Size: 110 217 3992 187 2869
Fragments arranged by size:
3992 2869 217 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7375
BssHII G'CGCG_C
Cuts at: 1925 3817 1925
Size: 1892 5483
BssSI C'ACGA_G
Cuts at: 3518 5435 6447 3518
Size: 1917 1012 4446
Fragments arranged by size:
4446 1917 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3281 794
Size: 2487 4888
BstBI TT'CG_AA
Cuts at: 6179 6179
Size: 7375
BstEII G'GTnAC_C
Cuts at: 1184 2712 1184
Size: 1528 5847
BstXI CCAn_nnnn'nTGG
Cuts at: 2123 3520 3943 4189 2123
Size: 1397 423 246 5309
Fragments arranged by size:
5309 1397 423 246
BstYI r'GATC_y
Cuts at: 1972 3220 3259 4705 5072 5457 5474 6926 7012
Size: 1248 39 1446 367 385 17 1452 86
Cuts at: 7012 7024 7370 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1446 1248 385 367 346 86
39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2643 3055 2643
Size: 412 6963
BtgI C'CryG_G
Cuts at: 513 1065 2577 2784 513
Size: 552 1512 207 5104
Fragments arranged by size:
5104 1512 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3379 4102 487
Size: 683 56 2153 723 3760
Fragments arranged by size:
3760 2153 723 683 56
BtsI GCAGTG_nn'
Cuts at: 3725 4631 4891 5685 5705 3725
Size: 906 260 794 20 5395
Fragments arranged by size:
5395 906 794 260 20
ClaI AT'CG_AT
Cuts at: 1554 3504 1554
Size: 1950 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7340
DraI TTT'AAA
Cuts at: 4330 4679 4330
Size: 349 7026
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2105 6382 809
Size: 507 789 4277 1802
Fragments arranged by size:
4277 1802 789 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2313 3774
Size: 54 1425 50 275 18 199 284 1461
Cuts at: 3774 4072 4822 5711 7340 8
Size: 298 750 889 1629 43
Fragments arranged by size:
1629 1461 1425 889 750 298 284 275
199 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4822 1537
Size: 275 3010 4090
Fragments arranged by size:
4090 3010 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2459 2501 2610 3861 4286 4519 5305 1353
Size: 1106 42 109 1251 425 233 786 3423
Fragments arranged by size:
3423 1251 1106 786 425 233 109 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2102 4208 5962 6336 6482 387
Size: 1064 138 513 2106 1754 374 146 1280
Fragments arranged by size:
2106 1754 1280 1064 513 374 146 138
EcoICRI GAG'CTC
Cuts at: 2056 2565 3094 2056
Size: 509 529 6337
Fragments arranged by size:
6337 529 509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2525 2609 2931 3068 3246 3476 3787
Size: 393 893 84 322 137 178 230 311
Cuts at: 3787 4563 4588 5438 6035 6822 7090 1239
Size: 776 25 850 597 787 268 1524
Fragments arranged by size:
1524 893 850 787 776 597 393 322
311 268 230 178 137 84 25
EcoNI CCTnn'n_nnAGG
Cuts at: 4094 4094
Size: 7375
EcoO109I rG'GnC_Cy
Cuts at: 2173 3382 3415 4253 4254 2173
Size: 1209 33 838 1 5294
Fragments arranged by size:
5294 1209 838 33 1
EcoRI G'AATT_C
Cuts at: 4684 5062 4684
Size: 378 6997
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4593 4593
Size: 7375
FspI TGC'GCA
Cuts at: 5881 5881
Size: 7375
HaeII r_GCGC'y
Cuts at: 1678 3071 6522 1678
Size: 1393 3451 2531
Fragments arranged by size:
3451 2531 1393
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2426 2458 2659 2691 3127
Size: 32 135 32 854 32 201 32 436
Cuts at: 3127 3159 6016 6048 6090 6122 1373
Size: 32 2857 32 42 32 2626
Fragments arranged by size:
2857 2626 854 436 201 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2200 4225 4468 4543 4719 4965
Size: 988 281 262 2025 243 75 176 246
Cuts at: 4965 669
Size: 3079
Fragments arranged by size:
3079 2025 988 281 262 246 243 176
75
HindIII A'AGCT_T
Cuts at: 748 3913 748
Size: 3165 4210
HpaI GTT'AAC
Cuts at: 4965 4965
Size: 7375
KpnI G_GTAC'C
Cuts at: 4700 4700
Size: 7375
MfeI C'AATT_G
Cuts at: 4974 4974
Size: 7375
MluI A'CGCG_T
Cuts at: 7251 7251
Size: 7375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6489 6673 1305
Size: 5184 184 2007
Fragments arranged by size:
5184 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2315 3776 4074 10
Size: 54 1425 343 483 1461 298 3311
Fragments arranged by size:
3311 1461 1425 483 343 298 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2558 3180 3215 4187 5333 5692 5851
Size: 648 1392 622 35 972 1146 359 159
Cuts at: 5851 518
Size: 2042
Fragments arranged by size:
2042 1392 1146 972 648 622 359 159
35
MspA1I CmG'CkG
Cuts at: 1606 2362 2494 3824 3984 4412 4673 4791 5468
Size: 756 132 1330 160 428 261 118 677
Cuts at: 5468 6616 6861 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1330 1148 756 677 428 261 245
160 132 118
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 7375
NcoI C'CATG_G
Cuts at: 513 1065 2577 513
Size: 552 1512 5311
Fragments arranged by size:
5311 1512 552
NdeI CA'TA_TG
Cuts at: 387 2235 387
Size: 1848 5527
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 7375
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7375
NotI GC'GGCC_GC
Cuts at: 4822 4822
Size: 7375
NsiI A_TGCA'T
Cuts at: 3451 3451
Size: 7375
NspI r_CATG'y
Cuts at: 1132 1229 2548 3284 4499 4733 6278 1132
Size: 97 1319 736 1215 234 1545 2229
Fragments arranged by size:
2229 1545 1319 1215 736 234 97
PasI CC'CwG_GG
Cuts at: 1348 4249 1348
Size: 2901 4474
PciI A'CATG_T
Cuts at: 2544 6274 2544
Size: 3730 3645
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7375
PfoI T'CCnGG_A
Cuts at: 3149 3453 3149
Size: 304 7071
PmeI GTTT'AAAC
Cuts at: 4679 4679
Size: 7375
PmlI CAC'GTG
Cuts at: 3978 3978
Size: 7375
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2531 2563 5444 5476 6957 6989 2531
Size: 32 2881 32 1481 32 2917
Fragments arranged by size:
2917 2881 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2173 3382 2173
Size: 1209 6166
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7375
PsiI TTA'TAA
Cuts at: 3546 4945 3546
Size: 1399 5976
PspOMI G'GGCC_C
Cuts at: 4253 4253
Size: 7375
PstI C_TGCA'G
Cuts at: 830 4727 830
Size: 3897 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7375
PvuII CAG'CTG
Cuts at: 2362 2494 3824 4412 2362
Size: 132 1330 588 5325
Fragments arranged by size:
5325 1330 588 132
SacI G_AGCT'C
Cuts at: 2058 2567 3096 2058
Size: 509 529 6337
Fragments arranged by size:
6337 529 509
SalI G'TCGA_C
Cuts at: 1936 4541 4717 1936
Size: 2605 176 4594
Fragments arranged by size:
4594 2605 176
SbfI CC_TGCA'GG
Cuts at: 4727 4727
Size: 7375
ScaI AGT'ACT
Cuts at: 5623 7335 5623
Size: 1712 5663
SfcI C'TryA_G
Cuts at: 826 1045 2454 4723 5858 6539 6730 826
Size: 219 1409 2269 1135 681 191 1471
Fragments arranged by size:
2269 1471 1409 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7375
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7375
SmaI CCC'GGG
Cuts at: 1872 4702 1872
Size: 2830 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5479 6380 6642 820
Size: 197 926 3536 901 262 1553
Fragments arranged by size:
3536 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5166 493
Size: 4673 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7375
SphI G_CATG'C
Cuts at: 1132 4733 1132
Size: 3601 3774
SspI AAT'ATT
Cuts at: 5 52 3542 4458 5299 5
Size: 47 3490 916 841 2081
Fragments arranged by size:
3490 2081 916 841 47
StuI AGG'CCT
Cuts at: 1517 2394 3587 1517
Size: 877 1193 5305
Fragments arranged by size:
5305 1193 877
StyI C'CwwG_G
Cuts at: 513 1065 2577 3970 4090 4814 513
Size: 552 1512 1393 120 724 3074
Fragments arranged by size:
3074 1512 1393 724 552 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2731 5402 5419 5572 5757 6202 325
Size: 1255 1151 2671 17 153 185 445 1498
Fragments arranged by size:
2671 1498 1255 1151 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5621 7333 96
Size: 274 80 33 51 1443 3644 1712 138
Fragments arranged by size:
3644 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2693 2710 2725 2742 3555 3587 7335 7367 2693
Size: 17 15 17 813 32 3748 32 2701
Fragments arranged by size:
3748 2701 813 32 32 17 17 15
Tth111I GACn'n_nGTC
Cuts at: 1316 2440 3191 1316
Size: 1124 751 5500
Fragments arranged by size:
5500 1124 751
XbaI T'CTAG_A
Cuts at: 4711 4711
Size: 7375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2156 2584 2817 2011
Size: 145 428 233 6569
Fragments arranged by size:
6569 428 233 145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7375
XmaI C'CCGG_G
Cuts at: 1870 4700 1870
Size: 2830 4545
XmnI GAAnn'nnTTC
Cuts at: 4573 5504 4573
Size: 931 6444
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5183 276
Size: 53 83 186 936 3649 2468
Fragments arranged by size:
3649 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI
PacI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI