Restriction map for
pFN21AA0187
(Circular) (Six-base) MAPSORT of: pFN21AA0187.seq Check: 1417 from: 1 to: 8551
pFN21AA0187 8551 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5703 5703
Size: 8551
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6361 278
Size: 53 83 186 936 4825 2468
Fragments arranged by size:
4825 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4916 5894 1937
Size: 2979 978 4594
Fragments arranged by size:
4594 2979 978
Acc65I G'GTAC_C
Cuts at: 5872 5872
Size: 8551
AclI AA'CG_TT
Cuts at: 1563 2495 6678 7051 1563
Size: 932 4183 373 3063
Fragments arranged by size:
4183 3063 932 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 3378 3777 3795 3807 4802 5698 5764
Size: 977 769 399 18 12 995 896 66
Cuts at: 5764 5768 6614 7998 8266 1632
Size: 4 846 1384 268 1917
Fragments arranged by size:
1917 1384 995 977 896 846 769 399
268 66 18 12 4
AfeI AGC'GCT
Cuts at: 2981 2981
Size: 8551
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8354
AflIII A'CryG_T
Cuts at: 1203 7450 8427 1203
Size: 6247 977 1327
Fragments arranged by size:
6247 1327 977
AgeI A'CCGG_T
Cuts at: 7382 7382
Size: 8551
AhdI GACnn_n'nnGTC
Cuts at: 5380 5594 7280 5380
Size: 214 1686 6651
Fragments arranged by size:
6651 1686 214
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8551
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5293 5325 5293
Size: 32 8519
AlwNI CAG_nnn'CTG
Cuts at: 2622 3889 6294 7866 2622
Size: 1267 2405 1572 3307
Fragments arranged by size:
3307 2405 1572 1267
ApaI G_GGCC'C
Cuts at: 3108 4958 3108
Size: 1850 6701
ApaLI G'TGCA_C
Cuts at: 4376 6608 7764 4376
Size: 2232 1156 5163
Fragments arranged by size:
5163 2232 1156
ApoI r'AATT_y
Cuts at: 1493 2892 3931 4598 4691 5109 5168 5784 5860
Size: 1399 1039 667 93 418 59 616 76
Cuts at: 5860 6086 6238 8347 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1399 1039 667 616 418 226
152 93 76 59
AseI AT'TA_AT
Cuts at: 160 1032 7105 160
Size: 872 6073 1606
Fragments arranged by size:
6073 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8551
AvaI C'yCGr_G
Cuts at: 1870 1943 2638 4818 5876 1870
Size: 73 695 2180 1058 4545
Fragments arranged by size:
4545 2180 1058 695 73
BamHI G'GATC_C
Cuts at: 5881 6248 5881
Size: 367 8184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2642 3992 5569 5691 5872
Size: 325 217 627 855 1350 1577 122 181
Cuts at: 5872 7327 618
Size: 1455 1842
Fragments arranged by size:
1842 1577 1455 1350 855 627 325 217
181 122
BanII G_rGCy'C
Cuts at: 1391 2244 3108 3539 4341 4958 5236 5870 1391
Size: 853 864 431 802 617 278 634 4072
Fragments arranged by size:
4072 864 853 802 634 617 431 278
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2201 2600 3486 3825 4017 4362 4890
Size: 971 302 399 886 339 192 345 528
Cuts at: 4890 4903 5284 928
Size: 13 381 4195
Fragments arranged by size:
4195 971 886 528 399 381 345 339
302 192 13
BbvCI CC'TCA_GC
Cuts at: 4032 5835 4032
Size: 1803 6748
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2093 2127 6742 6776 1903
Size: 34 156 34 4615 34 3678
Fragments arranged by size:
4615 3678 156 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5319 6443 7653 5319
Size: 1124 1210 6217
Fragments arranged by size:
6217 1210 1124
BclI T'GATC_A
Cuts at: 1548 3427 1548
Size: 1879 6672
BfrBI ATG'CAT
Cuts at: 4933 4933
Size: 8551
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3164 7162
Size: 107 122 71 631 605 337 1155 3998
Cuts at: 7162 136
Size: 1525
Fragments arranged by size:
3998 1525 1155 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2446 8546 2446
Size: 6100 2451
BlpI GC'TnA_GC
Cuts at: 3538 5968 3538
Size: 2430 6121
Bme1580I G_kGCm'C
Cuts at: 1790 3108 3997 4380 4958 6612 7768 1790
Size: 1318 889 383 578 1654 1156 2573
Fragments arranged by size:
2573 1654 1318 1156 889 578 383
BmgBI CAC'GTC
Cuts at: 1318 4375 1318
Size: 3057 5494
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3899 4246 5333
Size: 416 53 83 399 24 2482 347 1087
Cuts at: 5333 6282 7240 442
Size: 949 958 1753
Fragments arranged by size:
2482 1753 1087 958 949 416 399 347
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8551
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3553 3553
Size: 8551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 3045 3342 3654 3713 4192 5220 7211
Size: 836 970 297 312 59 479 1028 1991
Cuts at: 7211 1239
Size: 2579
Fragments arranged by size:
2579 1991 1028 970 836 479 312 297
59
Bpu10I CC'TnA_GC
Cuts at: 2013 4032 5835 2013
Size: 2019 1803 4729
Fragments arranged by size:
4729 2019 1803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3488 3548 3852 3983 4796 6676 7541 7839 3488
Size: 60 304 131 813 1880 865 298 4200
Fragments arranged by size:
4200 1880 865 813 304 298 131 60
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3908 5581 7214 882
Size: 617 2409 1673 1633 2219
Fragments arranged by size:
2409 2219 1673 1633 617
BsaAI yAC'GTr
Cuts at: 493 1204 4768 6342 493
Size: 711 3564 1574 2702
Fragments arranged by size:
3564 2702 1574 711
BsaBI GATnn'nnATC
Cuts at: 8545 8545
Size: 8551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5424 6358 6740 7375
Size: 53 83 186 936 3891 934 382 635
Cuts at: 7375 275
Size: 1451
Fragments arranged by size:
3891 1451 936 934 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2835 6984 7382 7656 7803 8214 2835
Size: 4149 398 274 147 411 3172
Fragments arranged by size:
4149 3172 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2549 2579 1178
Size: 30 165 30 1146 30 7150
Fragments arranged by size:
7150 1146 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2880 3419 3547 3787 4262 4349 4864 5747
Size: 1694 539 128 240 475 87 515 883
Cuts at: 5747 5750 5753 1186
Size: 3 3 3984
Fragments arranged by size:
3984 1694 883 539 515 475 240 128
87 3 3
BseYI C'CCAG_C
Cuts at: 5323 7754 8250 5323
Size: 2431 496 5624
Fragments arranged by size:
5624 2431 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3577 3828 4718 4790 5480 5681 1163
Size: 573 1841 251 890 72 690 201 4033
Fragments arranged by size:
4033 1841 890 690 573 251 201 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5595 6001 6762 7790 1540
Size: 275 186 3594 406 761 1028 2301
Fragments arranged by size:
3594 2301 1028 761 406 275 186
BsiHKAI G_wGCw'C
Cuts at: 2294 2872 3539 4341 4380 5236 5712 6612 6697
Size: 578 667 802 39 856 476 900 85
Cuts at: 6697 7768 2294
Size: 1071 3077
Fragments arranged by size:
3077 1071 900 856 802 667 578 476
85 39
BsmI GAATG_Cn'
Cuts at: 3288 5394 6062 6155 3288
Size: 2106 668 93 5684
Fragments arranged by size:
5684 2106 668 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2094 2094
Size: 8551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2294 2872 3108 3539 3997 4341
Size: 399 454 50 578 236 431 458 344
Cuts at: 4341 4380 4958 5236 5712 5870 6612 6697 7768
Size: 39 578 278 476 158 742 85 1071
Cuts at: 7768 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 578 578 476 458 454
431 399 344 278 236 158 85 50
39
BspEI T'CCGG_A
Cuts at: 8214 8214
Size: 8551
BspHI T'CATG_A
Cuts at: 5611 6440 8170 5611
Size: 829 1730 5992
Fragments arranged by size:
5992 1730 829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5703 5839 5906 844
Size: 4859 136 67 3489
Fragments arranged by size:
4859 3489 136 67
BsrBI CCG'CTC
Cuts at: 1394 1823 6438 1394
Size: 429 4615 3507
Fragments arranged by size:
4615 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3745 4933 7046 7220 65
Size: 1183 2497 1188 2113 174 1396
Fragments arranged by size:
2497 2113 1396 1188 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5590 7195 7382 1700
Size: 110 3780 1605 187 2869
Fragments arranged by size:
3780 2869 1605 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8551
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8551
BssSI C'ACGA_G
Cuts at: 6611 7623 6611
Size: 1012 7539
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8551
BstBI TT'CG_AA
Cuts at: 5188 7355 5188
Size: 2167 6384
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8551
BstXI CCAn_nnnn'nTGG
Cuts at: 4729 4729
Size: 8551
BstYI r'GATC_y
Cuts at: 1972 2086 2446 4822 5622 5881 6248 6633 6650
Size: 114 360 2376 800 259 367 385 17
Cuts at: 6650 8102 8188 8200 8546 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2376 1977 1452 800 385 367 360 346
259 114 86 17 12
BstZ17I GTA'TAC
Cuts at: 4917 4917
Size: 8551
Bsu36I CC'TnA_GG
Cuts at: 5468 5765 5468
Size: 297 8254
BtgI C'CryG_G
Cuts at: 513 1065 2004 5397 5419 513
Size: 552 939 3393 22 3645
Fragments arranged by size:
3645 3393 939 552 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4775 487
Size: 683 56 3549 4263
Fragments arranged by size:
4263 3549 683 56
BtsI GCAGTG_nn'
Cuts at: 3583 3594 3883 5492 5630 6067 6861 6881 3583
Size: 11 289 1609 138 437 794 20 5253
Fragments arranged by size:
5253 1609 794 437 289 138 20 11
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8516
DraI TTT'AAA
Cuts at: 2988 5855 2988
Size: 2867 5684
DraIII CAC_nnn'GTG
Cuts at: 3806 3806
Size: 8551
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2365 7558 809
Size: 507 1049 5193 1802
Fragments arranged by size:
5193 1802 1049 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5163 5592 5998
Size: 54 1425 50 275 18 3333 429 406
Cuts at: 5998 6887 8516 8
Size: 889 1629 43
Fragments arranged by size:
3333 1629 1425 889 429 406 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5592 5998 1537
Size: 275 3780 406 4090
Fragments arranged by size:
4090 3780 406 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 2964 3396 3579 3753 3828 4128 4484
Size: 761 850 432 183 174 75 300 356
Cuts at: 4484 5783 6481 1353
Size: 1299 698 3423
Fragments arranged by size:
3423 1299 850 761 698 432 356 300
183 174 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5599 7138 7512 7658 387
Size: 1064 138 4010 1539 374 146 1280
Fragments arranged by size:
4010 1539 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3537 4339 5234 3537
Size: 802 895 6854
Fragments arranged by size:
6854 895 802
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2609 3045 3342 3378 3654 3713
Size: 393 443 534 436 297 36 276 59
Cuts at: 3713 3777 3795 3807 4192 4802 5220 5698 5764
Size: 64 18 12 385 610 418 478 66
Cuts at: 5764 5768 6614 7211 7998 8266 1239
Size: 4 846 597 787 268 1524
Fragments arranged by size:
1524 846 787 610 597 534 478 443
436 418 393 385 297 276 268 66
64 59 36 18 12 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2176 4042 2176
Size: 1866 6685
EcoO109I rG'GnC_Cy
Cuts at: 2270 2351 3104 4225 4394 4954 4955 5521 5761
Size: 81 753 1121 169 560 1 566 240
Cuts at: 5761 2270
Size: 5060
Fragments arranged by size:
5060 1121 753 566 560 240 169 81
1
EcoRI G'AATT_C
Cuts at: 5784 5860 6238 5784
Size: 76 378 8097
Fragments arranged by size:
8097 378 76
EcoRV GAT'ATC
Cuts at: 2812 2812
Size: 8551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5794 5794
Size: 8551
FspI TGC'GCA
Cuts at: 7057 7057
Size: 8551
HaeII r_GCGC'y
Cuts at: 1678 2983 7698 1678
Size: 1305 4715 2531
Fragments arranged by size:
4715 2531 1305
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2257 2289 2413 2445 3338
Size: 32 135 32 685 32 124 32 893
Cuts at: 3338 3370 3412 3444 3865 3897 4812 4844 5366
Size: 32 42 32 421 32 915 32 522
Cuts at: 5366 5398 5580 5612 7192 7224 7266 7298 1373
Size: 32 182 32 1580 32 42 32 2626
Fragments arranged by size:
2626 1580 915 893 685 522 421 182
135 124 42 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2337 3067 4159 5895 6141 669
Size: 988 281 399 730 1092 1736 246 3079
Fragments arranged by size:
3079 1736 1092 988 730 399 281 246
HindIII A'AGCT_T
Cuts at: 748 2133 3851 4076 748
Size: 1385 1718 225 5223
Fragments arranged by size:
5223 1718 1385 225
HpaI GTT'AAC
Cuts at: 6141 6141
Size: 8551
KpnI G_GTAC'C
Cuts at: 5876 5876
Size: 8551
MfeI C'AATT_G
Cuts at: 5843 6150 5843
Size: 307 8244
MluI A'CGCG_T
Cuts at: 8427 8427
Size: 8551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2263 3269 3351 3453 7665 7849 1305
Size: 958 1006 82 102 4212 184 2007
Fragments arranged by size:
4212 2007 1006 958 184 102 82
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5165 10
Size: 54 1425 343 3333 3396
Fragments arranged by size:
3396 3333 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 6509 6868 7027 518
Size: 648 1251 4092 359 159 2042
Fragments arranged by size:
4092 2042 1251 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3542 3755 4031 4585 5689 5967 6644 7792
Size: 1936 213 276 554 1104 278 677 1148
Cuts at: 7792 8037 1606
Size: 245 2120
Fragments arranged by size:
2120 1936 1148 1104 677 554 278 276
245 213
NcoI C'CATG_G
Cuts at: 513 1065 2004 5419 513
Size: 552 939 3415 3645
Fragments arranged by size:
3645 3415 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8551
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8551
NotI GC'GGCC_GC
Cuts at: 5998 5998
Size: 8551
NsiI A_TGCA'T
Cuts at: 4935 4935
Size: 8551
NspI r_CATG'y
Cuts at: 1132 1229 2902 4933 5909 7454 1132
Size: 97 1673 2031 976 1545 2229
Fragments arranged by size:
2229 2031 1673 1545 976 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8551
PciI A'CATG_T
Cuts at: 7450 7450
Size: 8551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2282 2707 3118 1108
Size: 1174 425 411 6541
Fragments arranged by size:
6541 1174 425 411
PmeI GTTT'AAAC
Cuts at: 5855 5855
Size: 8551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5432 5464 6620 6652 8133 8165 5432
Size: 32 1156 32 1481 32 5818
Fragments arranged by size:
5818 1481 1156 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2270 5761 2270
Size: 3491 5060
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8551
PsiI TTA'TAA
Cuts at: 6121 6121
Size: 8551
PspOMI G'GGCC_C
Cuts at: 3104 4954 3104
Size: 1850 6701
PstI C_TGCA'G
Cuts at: 830 2085 3601 5903 830
Size: 1255 1516 2302 3478
Fragments arranged by size:
3478 2302 1516 1255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8551
PvuII CAG'CTG
Cuts at: 3755 4031 4585 3755
Size: 276 554 7721
Fragments arranged by size:
7721 554 276
SacI G_AGCT'C
Cuts at: 3539 4341 5236 3539
Size: 802 895 6854
Fragments arranged by size:
6854 895 802
SalI G'TCGA_C
Cuts at: 1936 5893 1936
Size: 3957 4594
SapI GCTCTTCn'nnn_
Cuts at: 2964 4128 5783 2964
Size: 1164 1655 5732
Fragments arranged by size:
5732 1655 1164
SbfI CC_TGCA'GG
Cuts at: 5903 5903
Size: 8551
ScaI AGT'ACT
Cuts at: 6799 8511 6799
Size: 1712 6839
SexAI A'CCwGG_T
Cuts at: 5378 5378
Size: 8551
SfcI C'TryA_G
Cuts at: 826 1045 2081 3597 4839 5034 5899 7034 7715
Size: 219 1036 1516 1242 195 865 1135 681
Cuts at: 7715 7906 826
Size: 191 1471
Fragments arranged by size:
1516 1471 1242 1135 1036 865 681 219
195 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8551
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8551
SmaI CCC'GGG
Cuts at: 1872 2640 5878 1872
Size: 768 3238 4545
Fragments arranged by size:
4545 3238 768
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3467 3527 3867 3998 4811 6655
Size: 197 926 1524 60 340 131 813 1844
Cuts at: 6655 7556 7818 820
Size: 901 262 1553
Fragments arranged by size:
1844 1553 1524 926 901 813 340 262
197 131 60
SnaBI TAC'GTA
Cuts at: 493 6342 493
Size: 5849 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8551
SphI G_CATG'C
Cuts at: 1132 5909 1132
Size: 4777 3774
SspI AAT'ATT
Cuts at: 5 52 6475 5
Size: 47 6423 2081
Fragments arranged by size:
6423 2081 47
StuI AGG'CCT
Cuts at: 1517 2740 4037 1517
Size: 1223 1297 6031
Fragments arranged by size:
6031 1297 1223
StyI C'CwwG_G
Cuts at: 513 1065 2004 3070 4314 5154 5419 5679 5990
Size: 552 939 1066 1244 840 265 260 311
Cuts at: 5990 513
Size: 3074
Fragments arranged by size:
3074 1244 1066 939 840 552 311 265
260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2354 2394 2570 3125 4721 5354 6578
Size: 1255 774 40 176 555 1596 633 1224
Cuts at: 6578 6595 6748 6933 7378 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1596 1498 1255 1224 774 633 555 445
185 176 153 40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2521 6797 8509
Size: 274 80 33 51 1443 544 4276 1712
Cuts at: 8509 96
Size: 138
Fragments arranged by size:
4276 1712 1443 544 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2549 2581 8511 8543 2549
Size: 32 5930 32 2557
Fragments arranged by size:
5930 2557 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3056 1316
Size: 1740 6811
XbaI T'CTAG_A
Cuts at: 4129 5887 4129
Size: 1758 6793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3115 4216 3115
Size: 1101 7450
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8551
XmaI C'CCGG_G
Cuts at: 1870 2638 5876 1870
Size: 768 3238 4545
Fragments arranged by size:
4545 3238 768
XmnI GAAnn'nnTTC
Cuts at: 4680 5777 6680 4680
Size: 1097 903 6551
Fragments arranged by size:
6551 1097 903
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6359 276
Size: 53 83 186 936 4825 2468
Fragments arranged by size:
4825 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BsiWI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI