Restriction map for
pFN21AA0182
(Circular) (Six-base) MAPSORT of: pFN21AA0182.seq Check: 4484 from: 1 to: 8137
pFN21AA0182 8137 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2319 2688 3337 5209 2319
Size: 369 649 1872 5247
Fragments arranged by size:
5247 1872 649 369
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5947 278
Size: 53 83 186 936 4411 2468
Fragments arranged by size:
4411 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4322 5239 5480 1937
Size: 2385 917 241 4594
Fragments arranged by size:
4594 2385 917 241
Acc65I G'GTAC_C
Cuts at: 5458 5458
Size: 8137
AclI AA'CG_TT
Cuts at: 1563 6264 6637 1563
Size: 4701 373 3063
Fragments arranged by size:
4701 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2563 3125 3923 5441 6200 7584 7852 1632
Size: 931 562 798 1518 759 1384 268 1917
Fragments arranged by size:
1917 1518 1384 931 798 759 562 268
AfeI AGC'GCT
Cuts at: 2736 4414 2736
Size: 1678 6459
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7940
AflIII A'CryG_T
Cuts at: 1203 4146 4959 7036 8013 1203
Size: 2943 813 2077 977 1327
Fragments arranged by size:
2943 2077 1327 977 813
AgeI A'CCGG_T
Cuts at: 6968 6968
Size: 8137
AhdI GACnn_n'nnGTC
Cuts at: 3319 6866 3319
Size: 3547 4590
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8137
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 8105
AlwNI CAG_nnn'CTG
Cuts at: 3352 3364 4663 5880 7452 3352
Size: 12 1299 1217 1572 4037
Fragments arranged by size:
4037 1572 1299 1217 12
ApaI G_GGCC'C
Cuts at: 2105 2965 3798 3804 3943 4105 5060 2105
Size: 860 833 6 139 162 955 5182
Fragments arranged by size:
5182 955 860 833 162 139 6
ApaLI G'TGCA_C
Cuts at: 2399 6194 7350 2399
Size: 3795 1156 3186
Fragments arranged by size:
3795 3186 1156
ApoI r'AATT_y
Cuts at: 1493 5446 5672 5824 7933 1493
Size: 3953 226 152 2109 1697
Fragments arranged by size:
3953 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6691 160
Size: 872 5659 1606
Fragments arranged by size:
5659 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8137
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 2964 3090 3791 3797 3818 5462
Size: 73 765 256 126 701 6 21 1644
Cuts at: 5462 1870
Size: 4545
Fragments arranged by size:
4545 1644 765 701 256 126 73 21
6
AvrII C'CTAG_G
Cuts at: 5408 5408
Size: 8137
BamHI G'GATC_C
Cuts at: 5467 5834 5467
Size: 367 7770
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2410 3124 3157 3178 3385
Size: 325 217 627 623 714 33 21 207
Cuts at: 3385 5458 6913 618
Size: 2073 1455 1842
Fragments arranged by size:
2073 1842 1455 714 627 623 325 217
207 33 21
BanII G_rGCy'C
Cuts at: 1391 2105 2265 2715 2965 3003 3627 3798 3804
Size: 714 160 450 250 38 624 171 6
Cuts at: 3804 3819 3891 3943 4105 4836 5040 5060 5456
Size: 15 72 52 162 731 204 20 396
Cuts at: 5456 1391
Size: 4072
Fragments arranged by size:
4072 731 714 624 450 396 250 204
171 162 160 72 52 38 20 15
6
BbeI G_GCGC'C
Cuts at: 3128 3128
Size: 8137
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3661 928
Size: 971 1762 5404
Fragments arranged by size:
5404 1762 971
BbvCI CC'TCA_GC
Cuts at: 2762 5267 2762
Size: 2505 5632
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2662 2696 6328 6362 1903
Size: 34 725 34 3632 34 3678
Fragments arranged by size:
3678 3632 725 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6029 7239 6029
Size: 1210 6927
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8137
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2990 3922 6748
Size: 107 122 71 631 605 1318 932 2826
Cuts at: 6748 136
Size: 1525
Fragments arranged by size:
2826 1525 1318 932 631 605 122 107
71
BglII A'GATC_T
Cuts at: 8132 8132
Size: 8137
BlpI GC'TnA_GC
Cuts at: 2315 3813 3890 4712 5554 2315
Size: 1498 77 822 842 4898
Fragments arranged by size:
4898 1498 842 822 77
Bme1580I G_kGCm'C
Cuts at: 1790 2097 2105 2139 2403 2415 2965 3162 3183
Size: 307 8 34 264 12 550 197 21
Cuts at: 3183 3798 3804 3943 4105 5060 6198 7354 1790
Size: 615 6 139 162 955 1138 1156 2573
Fragments arranged by size:
2573 1156 1138 955 615 550 307 264
197 162 139 34 21 12 8 6
BmgBI CAC'GTC
Cuts at: 1318 4149 4962 1318
Size: 2831 813 4493
Fragments arranged by size:
4493 2831 813
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2412 3923 5868
Size: 416 53 83 399 24 995 1511 1945
Cuts at: 5868 6826 442
Size: 958 1753
Fragments arranged by size:
1945 1753 1511 995 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4685 1056
Size: 3629 4508
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5305 5305
Size: 8137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2206 2212 2372 2960 3017 3374 3791 4031
Size: 967 6 160 588 57 357 417 240
Cuts at: 4031 4746 4981 5231 5267 5336 6797 1239
Size: 715 235 250 36 69 1461 2579
Fragments arranged by size:
2579 1461 967 715 588 417 357 250
240 235 160 69 57 36 6
Bpu10I CC'TnA_GC
Cuts at: 2762 2795 4983 5267 2762
Size: 33 2188 284 5632
Fragments arranged by size:
5632 2188 284 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6262 7127 7425 6262
Size: 865 298 6974
Fragments arranged by size:
6974 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5338 6800 882
Size: 617 3839 1462 2219
Fragments arranged by size:
3839 2219 1462 617
BsaAI yAC'GTr
Cuts at: 493 1204 2407 5928 493
Size: 711 1203 3521 2702
Fragments arranged by size:
3521 2702 1203 711
BsaBI GATnn'nnATC
Cuts at: 8131 8131
Size: 8137
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3125 4298 5944 6326
Size: 53 83 186 936 1592 1173 1646 382
Cuts at: 6326 6961 275
Size: 635 1451
Fragments arranged by size:
1646 1592 1451 1173 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3154 6570 6968 7242 7389 7800 3154
Size: 3416 398 274 147 411 3491
Fragments arranged by size:
3491 3416 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2081 2149 2179 2218
Size: 30 165 30 648 30 68 30 39
Cuts at: 2218 2248 2370 2400 2738 2768 3257 3287 4031
Size: 30 122 30 338 30 489 30 744
Cuts at: 4031 4061 4495 4525 1178
Size: 30 434 30 4790
Fragments arranged by size:
4790 744 648 489 434 338 165 122
68 39 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2209 2804 3068 3278 3281 3704 4028 4103
Size: 1023 595 264 210 3 423 324 75
Cuts at: 4103 4106 5105 5108 5117 5144 5326 1186
Size: 3 999 3 9 27 182 3997
Fragments arranged by size:
3997 1023 999 595 423 324 264 210
182 75 27 9 3 3 3
BseYI C'CCAG_C
Cuts at: 2138 2345 2540 3002 3182 3510 3626 5016 7340
Size: 207 195 462 180 328 116 1390 2324
Cuts at: 7340 7836 2138
Size: 496 2439
Fragments arranged by size:
2439 2324 1390 496 462 328 207 195
180 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 2645 2666 2723 3507 3584 4755
Size: 573 642 267 21 57 784 77 1171
Cuts at: 4755 1163
Size: 4545
Fragments arranged by size:
4545 1171 784 642 573 267 77 57
21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5587 6348 7376 1540
Size: 275 186 3586 761 1028 2301
Fragments arranged by size:
3586 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2403 2715 3405 3419 3777 3819 3891 6198 6283
Size: 312 690 14 358 42 72 2307 85
Cuts at: 6283 7354 2403
Size: 1071 3186
Fragments arranged by size:
3186 2307 1071 690 358 312 85 72
42 14
BsmI GAATG_Cn'
Cuts at: 5648 5741 5648
Size: 93 8044
BsmBI CGTCTCn'nnnn_
Cuts at: 3566 4537 3566
Size: 971 7166
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2105 2139 2265 2403 2415 2715
Size: 399 307 8 34 126 138 12 300
Cuts at: 2715 2965 3003 3162 3183 3405 3419 3627 3777
Size: 250 38 159 21 222 14 208 150
Cuts at: 3777 3798 3804 3819 3891 3943 4105 4836 5040
Size: 21 6 15 72 52 162 731 204
Cuts at: 5040 5060 5456 6198 6283 7354 1391
Size: 20 396 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 731 399 396 307 300
250 222 208 204 162 159 150 138
126 85 72 52 38 34 21 21
20 15 14 12 8 6
BspEI T'CCGG_A
Cuts at: 7800 7800
Size: 8137
BspHI T'CATG_A
Cuts at: 6026 7756 6026
Size: 1730 6407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2319 2688 3337 5209 5492 844
Size: 1475 369 649 1872 283 3489
Fragments arranged by size:
3489 1872 1475 649 369 283
BsrBI CCG'CTC
Cuts at: 1394 1823 2269 2854 3058 3664 5215 5347 6024
Size: 429 446 585 204 606 1551 132 677
Cuts at: 6024 1394
Size: 3507
Fragments arranged by size:
3507 1551 677 606 585 446 429 204
132
BsrDI GCAATG_nn'
Cuts at: 65 1248 5403 6632 6806 65
Size: 1183 4155 1229 174 1396
Fragments arranged by size:
4155 1396 1229 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 2216 3315 3375 3388 3921 4582
Size: 110 395 11 1099 60 13 533 661
Cuts at: 4582 6781 6968 1700
Size: 2199 187 2869
Fragments arranged by size:
2869 2199 1099 661 533 395 187 110
60 13 11
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8137
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8137
BssSI C'ACGA_G
Cuts at: 3443 3753 4868 6197 7209 3443
Size: 310 1115 1329 1012 4371
Fragments arranged by size:
4371 1329 1115 1012 310
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3034 3157 3349 794
Size: 2240 123 192 5582
Fragments arranged by size:
5582 2240 192 123
BstBI TT'CG_AA
Cuts at: 4365 6941 4365
Size: 2576 5561
BstEII G'GTnAC_C
Cuts at: 1184 2155 1184
Size: 971 7166
BstXI CCAn_nnnn'nTGG
Cuts at: 2548 2995 5024 2548
Size: 447 2029 5661
Fragments arranged by size:
5661 2029 447
BstYI r'GATC_y
Cuts at: 1972 5467 5834 6219 6236 7688 7774 7786 8132
Size: 3495 367 385 17 1452 86 12 346
Cuts at: 8132 1972
Size: 1977
Fragments arranged by size:
3495 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2274 2612 2274
Size: 338 7799
BtgI C'CryG_G
Cuts at: 513 1065 2097 2151 3015 3199 3528 3698 3935
Size: 552 1032 54 864 184 329 170 237
Cuts at: 3935 4623 4826 513
Size: 688 203 3824
Fragments arranged by size:
3824 1032 864 688 552 329 237 203
184 170 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2146 2687 4098 487
Size: 683 56 920 541 1411 4526
Fragments arranged by size:
4526 1411 920 683 541 56
BtsI GCAGTG_nn'
Cuts at: 2239 2489 2517 2657 3232 4494 5043 5653 6447
Size: 250 28 140 575 1262 549 610 794
Cuts at: 6447 6467 2239
Size: 20 3909
Fragments arranged by size:
3909 1262 794 610 575 549 250 140
28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4094 4129 529
Size: 35 3530 35 4537
Fragments arranged by size:
4537 3530 35 35
DraI TTT'AAA
Cuts at: 5441 5441
Size: 8137
DraIII CAC_nnn'GTG
Cuts at: 2134 2407 2410 3178 5299 2134
Size: 273 3 768 2121 4972
Fragments arranged by size:
4972 2121 768 273 3
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3438 4964 7144 809
Size: 507 2122 1526 2180 1802
Fragments arranged by size:
2180 2122 1802 1526 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2514 2982
Size: 54 1425 50 275 18 222 462 468
Cuts at: 2982 3018 3040 3454 3873 4119 4491 5584 6473
Size: 36 22 414 419 246 372 1093 889
Cuts at: 6473 8102 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1093 889 468 462 419 414
372 275 246 222 54 50 43 36
22 18
EagI C'GGCC_G
Cuts at: 1537 1812 5584 1537
Size: 275 3772 4090
Fragments arranged by size:
4090 3772 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4415 4811 4842 5094 5193 6067 1353
Size: 3062 396 31 252 99 874 3423
Fragments arranged by size:
3423 3062 874 396 252 99 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3407 3732 4255 5318 6724 7098
Size: 1064 138 1818 325 523 1063 1406 374
Cuts at: 7098 7244 387
Size: 146 1280
Fragments arranged by size:
1818 1406 1280 1064 1063 523 374 325
146 138
EcoICRI GAG'CTC
Cuts at: 2713 3817 3889 2713
Size: 1104 72 6961
Fragments arranged by size:
6961 1104 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2212 2372 2563 2960 3017 3125
Size: 393 574 6 160 191 397 57 108
Cuts at: 3125 3374 3791 3923 4031 4746 4981 5231 5267
Size: 249 417 132 108 715 235 250 36
Cuts at: 5267 5336 5441 6200 6797 7584 7852 1239
Size: 69 105 759 597 787 268 1524
Fragments arranged by size:
1524 787 759 715 597 574 417 397
393 268 250 249 235 191 160 132
108 108 105 69 57 36 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3866 3866
Size: 8137
EcoO109I rG'GnC_Cy
Cuts at: 2013 2102 2616 2992 3801 3940 4101 4720 4897
Size: 89 514 376 809 139 161 619 177
Cuts at: 4897 5056 5057 2013
Size: 159 1 5093
Fragments arranged by size:
5093 809 619 514 376 177 161 159
139 89 1
EcoRI G'AATT_C
Cuts at: 5446 5824 5446
Size: 378 7759
EcoRV GAT'ATC
Cuts at: 5429 5429
Size: 8137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4457 4457
Size: 8137
FseI GG_CCGG'CC
Cuts at: 3379 3379
Size: 8137
FspI TGC'GCA
Cuts at: 6643 6643
Size: 8137
HaeII r_GCGC'y
Cuts at: 1678 2738 2947 3128 3985 4319 4416 4770 7284
Size: 1060 209 181 857 334 97 354 2514
Cuts at: 7284 1678
Size: 2531
Fragments arranged by size:
2531 2514 1060 857 354 334 209 181
97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2149 2181 2204 2236 2283
Size: 32 135 32 577 32 23 32 47
Cuts at: 2283 2315 2738 2770 3305 3337 4136 4168 4495
Size: 32 423 32 535 32 799 32 327
Cuts at: 4495 4507 4527 4539 4976 5008 5069 5101 6778
Size: 12 20 12 437 32 61 32 1677
Cuts at: 6778 6810 6852 6884 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1677 799 577 535 437 423 327
135 61 47 42 32 32 32 32
32 32 32 32 32 32 32 32
23 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4957 5481 5727 669
Size: 988 281 3019 524 246 3079
Fragments arranged by size:
3079 3019 988 524 281 246
HindIII A'AGCT_T
Cuts at: 748 2446 5178 748
Size: 1698 2732 3707
Fragments arranged by size:
3707 2732 1698
HpaI GTT'AAC
Cuts at: 5727 5727
Size: 8137
KasI G'GCGC_C
Cuts at: 3124 3124
Size: 8137
KpnI G_GTAC'C
Cuts at: 5462 5462
Size: 8137
MfeI C'AATT_G
Cuts at: 5736 5736
Size: 8137
MluI A'CGCG_T
Cuts at: 8013 8013
Size: 8137
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2192 3163 3219 5055 7251 7435 1305
Size: 887 971 56 1836 2196 184 2007
Fragments arranged by size:
2196 2007 1836 971 887 184 56
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3042 10
Size: 54 1425 343 1210 5105
Fragments arranged by size:
5105 1425 1210 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4787 6095 6454 6613 518
Size: 648 3621 1308 359 159 2042
Fragments arranged by size:
3621 2042 1308 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2392 2950 2959 3079 3289 3649 3853 3894
Size: 786 558 9 120 210 360 204 41
Cuts at: 3894 4625 4695 4795 5553 6230 7378 7623 1606
Size: 731 70 100 758 677 1148 245 2120
Fragments arranged by size:
2120 1148 786 758 731 677 558 360
245 210 204 120 100 70 41 9
NaeI GCC'GGC
Cuts at: 2207 2218 3377 3390 2207
Size: 11 1159 13 6954
Fragments arranged by size:
6954 1159 13 11
NarI GG'CG_CC
Cuts at: 3125 3125
Size: 8137
NcoI C'CATG_G
Cuts at: 513 1065 2097 3199 513
Size: 552 1032 1102 5451
Fragments arranged by size:
5451 1102 1032 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8137
NgoMIV G'CCGG_C
Cuts at: 2205 2216 3375 3388 2205
Size: 11 1159 13 6954
Fragments arranged by size:
6954 1159 13 11
NheI G'CTAG_C
Cuts at: 1052 4681 1052
Size: 3629 4508
NotI GC'GGCC_GC
Cuts at: 5584 5584
Size: 8137
NspI r_CATG'y
Cuts at: 1132 1229 2485 2755 4750 5495 7040 1132
Size: 97 1256 270 1995 745 1545 2229
Fragments arranged by size:
2229 1995 1545 1256 745 270 97
PasI CC'CwG_GG
Cuts at: 1348 2120 5433 1348
Size: 772 3313 4052
Fragments arranged by size:
4052 3313 772
PciI A'CATG_T
Cuts at: 7036 7036
Size: 8137
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5074 5299 1108
Size: 3966 225 3946
Fragments arranged by size:
3966 3946 225
PfoI T'CCnGG_A
Cuts at: 2222 4674 2222
Size: 2452 5685
PmeI GTTT'AAAC
Cuts at: 5441 5441
Size: 8137
PmlI CAC'GTG
Cuts at: 2407 2407
Size: 8137
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6206 6238 7719 7751 6206
Size: 32 1481 32 6592
Fragments arranged by size:
6592 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2013 2616 2013
Size: 603 7534
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8137
PsiI TTA'TAA
Cuts at: 5707 5707
Size: 8137
PspOMI G'GGCC_C
Cuts at: 2101 2961 3794 3800 3939 4101 5056 2101
Size: 860 833 6 139 162 955 5182
Fragments arranged by size:
5182 955 860 833 162 139 6
PstI C_TGCA'G
Cuts at: 830 2527 2781 3123 3978 4884 4905 4939 4992
Size: 1697 254 342 855 906 21 34 53
Cuts at: 4992 5489 830
Size: 497 3478
Fragments arranged by size:
3478 1697 906 855 497 342 254 53
34 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8137
PvuII CAG'CTG
Cuts at: 3079 3079
Size: 8137
SacI G_AGCT'C
Cuts at: 2715 3819 3891 2715
Size: 1104 72 6961
Fragments arranged by size:
6961 1104 72
SacII CC_GC'GG
Cuts at: 4626 4626
Size: 8137
SalI G'TCGA_C
Cuts at: 1936 5479 1936
Size: 3543 4594
SapI GCTCTTCn'nnn_
Cuts at: 4811 5193 4811
Size: 382 7755
SbfI CC_TGCA'GG
Cuts at: 3123 5489 3123
Size: 2366 5771
ScaI AGT'ACT
Cuts at: 6385 8097 6385
Size: 1712 6425
SfcI C'TryA_G
Cuts at: 826 1045 2523 2777 3119 3974 4330 4880 4901
Size: 219 1478 254 342 855 356 550 21
Cuts at: 4901 4935 4988 5260 5485 6620 7301 7492 826
Size: 34 53 272 225 1135 681 191 1471
Fragments arranged by size:
1478 1471 1135 855 681 550 356 342
272 254 225 219 191 53 34 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2990 2990
Size: 8137
SfoI GGC'GCC
Cuts at: 3126 3126
Size: 8137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8137
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8137
SmaI CCC'GGG
Cuts at: 1872 2966 3793 3799 5464 1872
Size: 1094 827 6 1665 4545
Fragments arranged by size:
4545 1665 1094 827 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3818 6241 7142 7404 820
Size: 197 926 1875 2423 901 262 1553
Fragments arranged by size:
2423 1875 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5928 493
Size: 5435 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8137
SphI G_CATG'C
Cuts at: 1132 4750 5495 1132
Size: 3618 745 3774
Fragments arranged by size:
3774 3618 745
SrfI GCCC'GGGC
Cuts at: 3793 3799 3793
Size: 6 8131
SspI AAT'ATT
Cuts at: 5 52 6061 5
Size: 47 6009 2081
Fragments arranged by size:
6009 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8137
StyI C'CwwG_G
Cuts at: 513 1065 2097 2988 3199 3567 4776 5408 5576
Size: 552 1032 891 211 368 1209 632 168
Cuts at: 5576 513
Size: 3074
Fragments arranged by size:
3074 1209 1032 891 632 552 368 211
168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2098 2444 2679 3541 3845 4208 4789
Size: 1255 518 346 235 862 304 363 581
Cuts at: 4789 4904 6164 6181 6334 6519 6964 325
Size: 115 1260 17 153 185 445 1498
Fragments arranged by size:
1498 1260 1255 862 581 518 445 363
346 304 235 185 153 115 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6383 8095 96
Size: 274 80 33 51 1443 4406 1712 138
Fragments arranged by size:
4406 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2218 2250 2370 2402 2547 2579 3257 3289 4848
Size: 32 120 32 145 32 678 32 1559
Cuts at: 4848 4880 8097 8129 2218
Size: 32 3217 32 2226
Fragments arranged by size:
3217 2226 1559 678 145 120 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8137
XbaI T'CTAG_A
Cuts at: 5473 5473
Size: 8137
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4808 4808
Size: 8137
XhoI C'TCGA_G
Cuts at: 1943 3818 1943
Size: 1875 6262
XmaI C'CCGG_G
Cuts at: 1870 2964 3791 3797 5462 1870
Size: 1094 827 6 1665 4545
Fragments arranged by size:
4545 1665 1094 827 6
XmnI GAAnn'nnTTC
Cuts at: 4379 6266 4379
Size: 1887 6250
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5945 276
Size: 53 83 186 936 4411 2468
Fragments arranged by size:
4411 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I FspAI NruI NsiI
PacI PspXI PsrI RsrII SanDI SexAI SwaI