Restriction map for
pFN21AA0179
(Circular) (Six-base) MAPSORT of: pFN21AA0179.seq Check: 8593 from: 1 to: 6925
pFN21AA0179 6925 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3183 4156 3183
Size: 973 5952
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4735 278
Size: 53 83 186 936 3199 2468
Fragments arranged by size:
3199 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4268 1937
Size: 2331 4594
Acc65I G'GTAC_C
Cuts at: 4246 4246
Size: 6925
AclI AA'CG_TT
Cuts at: 1563 5052 5425 1563
Size: 3489 373 3063
Fragments arranged by size:
3489 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2655 2976 3287 3863 3901 4148 4988 6372
Size: 1023 321 311 576 38 247 840 1384
Cuts at: 6372 6640 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1023 840 576 321 311 268
247 38
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6728
AflIII A'CryG_T
Cuts at: 1203 3274 3438 5824 6801 1203
Size: 2071 164 2386 977 1327
Fragments arranged by size:
2386 2071 1327 977 164
AgeI A'CCGG_T
Cuts at: 5756 5756
Size: 6925
AhdI GACnn_n'nnGTC
Cuts at: 5654 5654
Size: 6925
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3674 1184
Size: 2490 4435
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3820 3852 3820
Size: 32 6893
AlwNI CAG_nnn'CTG
Cuts at: 2050 3814 3856 3959 4668 6240 2050
Size: 1764 42 103 709 1572 2735
Fragments arranged by size:
2735 1764 1572 709 103 42
ApaI G_GGCC'C
Cuts at: 2832 3397 3681 4079 2832
Size: 565 284 398 5678
Fragments arranged by size:
5678 565 398 284
ApaLI G'TGCA_C
Cuts at: 4982 6138 4982
Size: 1156 5769
ApoI r'AATT_y
Cuts at: 1493 2939 4034 4234 4460 4612 6721 1493
Size: 1446 1095 200 226 152 2109 1697
Fragments arranged by size:
2109 1697 1446 1095 226 200 152
AseI AT'TA_AT
Cuts at: 160 1032 5479 160
Size: 872 4447 1606
Fragments arranged by size:
4447 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
AvaI C'yCGr_G
Cuts at: 1870 1943 2589 3242 3388 3454 4083 4250 1870
Size: 73 646 653 146 66 629 167 4545
Fragments arranged by size:
4545 653 646 629 167 146 73 66
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4039 4072 4039
Size: 33 6892
BamHI G'GATC_C
Cuts at: 4255 4622 4255
Size: 367 6558
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4246 5701 618
Size: 325 217 627 2459 1455 1842
Fragments arranged by size:
2459 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2172 2832 3243 3389 3397 3505 3681 3696
Size: 781 660 411 146 8 108 176 15
Cuts at: 3696 4079 4244 1391
Size: 383 165 4072
Fragments arranged by size:
4072 781 660 411 383 176 165 146
108 15 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2123 2590 2663 2747 3128 4049 4136
Size: 971 224 467 73 84 381 921 87
Cuts at: 4136 928
Size: 3717
Fragments arranged by size:
3717 971 921 467 381 224 87 84
73
BbvCI CC'TCA_GC
Cuts at: 2688 3857 2688
Size: 1169 5756
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3272 3306 5116 5150 1903
Size: 34 1335 34 1810 34 3678
Fragments arranged by size:
3678 1810 1335 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2722 4817 6027 2722
Size: 2095 1210 3620
Fragments arranged by size:
3620 2095 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6925
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3269 3459 3574
Size: 107 122 71 631 605 1597 190 115
Cuts at: 3574 5536 136
Size: 1962 1525
Fragments arranged by size:
1962 1597 1525 631 605 190 122 115
107 71
BglII A'GATC_T
Cuts at: 6920 6920
Size: 6925
BlpI GC'TnA_GC
Cuts at: 4342 4342
Size: 6925
Bme1580I G_kGCm'C
Cuts at: 1790 2832 3397 3543 3681 4079 4986 6142 1790
Size: 1042 565 146 138 398 907 1156 2573
Fragments arranged by size:
2573 1156 1042 907 565 398 146 138
BmgBI CAC'GTC
Cuts at: 1318 3303 1318
Size: 1985 4940
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2759 3321 4656
Size: 416 53 83 399 24 1342 562 1335
Cuts at: 4656 5614 442
Size: 958 1753
Fragments arranged by size:
1753 1342 1335 958 562 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6925
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2445 3343 3524 2445
Size: 898 181 5846
Fragments arranged by size:
5846 898 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2822 3324 3710 3806 3936 5585 1239
Size: 1583 502 386 96 130 1649 2579
Fragments arranged by size:
2579 1649 1583 502 386 130 96
Bpu10I CC'TnA_GC
Cuts at: 2688 3857 2688
Size: 1169 5756
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 2737 5050 5915 6213 2504
Size: 233 2313 865 298 3216
Fragments arranged by size:
3216 2313 865 298 233
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5588 882
Size: 617 4089 2219
Fragments arranged by size:
4089 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3275 4716 493
Size: 711 2071 1441 2702
Fragments arranged by size:
2702 2071 1441 711
BsaBI GATnn'nnATC
Cuts at: 2514 6919 2514
Size: 4405 2520
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 4732 5114 5749
Size: 53 83 186 936 521 2678 382 635
Cuts at: 5749 275
Size: 1451
Fragments arranged by size:
2678 1451 936 635 521 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5358 5756 6030 6177 6588 5358
Size: 398 274 147 411 5695
Fragments arranged by size:
5695 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 3934 3964 1178
Size: 30 165 30 993 30 1508 30 4139
Fragments arranged by size:
4139 1508 993 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2705 3332 1186
Size: 1519 627 4779
Fragments arranged by size:
4779 1519 627
BseYI C'CCAG_C
Cuts at: 2043 2358 3645 3707 3797 6128 6624 2043
Size: 315 1287 62 90 2331 496 2344
Fragments arranged by size:
2344 2331 1287 496 315 90 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3225 3312 1163
Size: 573 1489 87 4776
Fragments arranged by size:
4776 1489 573 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2028 4375 5136 6164 1540
Size: 275 186 27 2347 761 1028 2301
Fragments arranged by size:
2347 2301 1028 761 275 186 27
BsiHKAI G_wGCw'C
Cuts at: 2172 3696 3774 4986 5071 6142 2172
Size: 1524 78 1212 85 1071 2955
Fragments arranged by size:
2955 1524 1212 1071 85 78
BsmI GAATG_Cn'
Cuts at: 4436 4529 4436
Size: 93 6832
BsmBI CGTCTCn'nnnn_
Cuts at: 2633 2633
Size: 6925
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2832 3243 3389 3397 3505 3543
Size: 399 382 660 411 146 8 108 38
Cuts at: 3543 3681 3696 3774 4079 4244 4986 5071 6142
Size: 138 15 78 305 165 742 85 1071
Cuts at: 6142 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 660 411 399 382 305
165 146 138 108 85 78 38 15
8
BspEI T'CCGG_A
Cuts at: 6588 6588
Size: 6925
BspHI T'CATG_A
Cuts at: 4814 6544 4814
Size: 1730 5195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3183 4156 4280 844
Size: 2339 973 124 3489
Fragments arranged by size:
3489 2339 973 124
BsrBI CCG'CTC
Cuts at: 1394 1823 2084 4812 1394
Size: 429 261 2728 3507
Fragments arranged by size:
3507 2728 429 261
BsrDI GCAATG_nn'
Cuts at: 65 1248 2181 3581 5420 5594 65
Size: 1183 933 1400 1839 174 1396
Fragments arranged by size:
1839 1400 1396 1183 933 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 3568 5569 5756 1700
Size: 110 213 1545 2001 187 2869
Fragments arranged by size:
2869 2001 1545 213 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6925
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6925
BssSI C'ACGA_G
Cuts at: 4985 5997 4985
Size: 1012 5913
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3175 794
Size: 2381 4544
BstBI TT'CG_AA
Cuts at: 2601 5729 2601
Size: 3128 3797
BstEII G'GTnAC_C
Cuts at: 1184 2675 1184
Size: 1491 5434
BstXI CCAn_nnnn'nTGG
Cuts at: 4213 4213
Size: 6925
BstYI r'GATC_y
Cuts at: 1972 2031 4255 4622 5007 5024 6476 6562 6574
Size: 59 2224 367 385 17 1452 86 12
Cuts at: 6574 6920 1972
Size: 346 1977
Fragments arranged by size:
2224 1977 1452 385 367 346 86 59
17 12
Bsu36I CC'TnA_GG
Cuts at: 2324 3471 2324
Size: 1147 5778
BtgI C'CryG_G
Cuts at: 513 1065 2004 3374 4071 4117 513
Size: 552 939 1370 697 46 3321
Fragments arranged by size:
3321 1370 939 697 552 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6186
Fragments arranged by size:
6186 683 56
BtsI GCAGTG_nn'
Cuts at: 3426 3559 3829 3936 4441 5235 5255 3426
Size: 133 270 107 505 794 20 5096
Fragments arranged by size:
5096 794 505 270 133 107 20
ClaI AT'CG_AT
Cuts at: 1554 2529 1554
Size: 975 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3535 3570 3747 3782 529
Size: 35 2971 35 177 35 3672
Fragments arranged by size:
3672 2971 177 35 35 35
DraI TTT'AAA
Cuts at: 4229 4229
Size: 6925
DraIII CAC_nnn'GTG
Cuts at: 4123 4123
Size: 6925
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5932 809
Size: 507 4616 1802
Fragments arranged by size:
4616 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 3279 3371
Size: 54 1425 50 275 18 195 1254 92
Cuts at: 3371 4167 4372 5261 6890 8
Size: 796 205 889 1629 43
Fragments arranged by size:
1629 1425 1254 889 796 275 205 195
92 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2025 4372 1537
Size: 275 213 2347 4090
Fragments arranged by size:
4090 2347 275 213
EarI CTCTTCn'nnn_
Cuts at: 1353 2160 2631 2716 2791 3111 3377 3916 4855
Size: 807 471 85 75 320 266 539 939
Cuts at: 4855 1353
Size: 3423
Fragments arranged by size:
3423 939 807 539 471 320 266 85
75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5512 5886 6032 387
Size: 1064 138 3923 374 146 1280
Fragments arranged by size:
3923 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2170 3694 2170
Size: 1524 5401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2655 2822 2976 3287 3324 3710 3806
Size: 393 1023 167 154 311 37 386 96
Cuts at: 3806 3863 3901 3936 4148 4988 5585 6372 6640
Size: 57 38 35 212 840 597 787 268
Cuts at: 6640 1239
Size: 1524
Fragments arranged by size:
1524 1023 840 787 597 393 386 311
268 212 167 154 96 57 38 37
35
EcoNI CCTnn'n_nnAGG
Cuts at: 2399 3603 2399
Size: 1204 5721
EcoO109I rG'GnC_Cy
Cuts at: 2407 2828 2829 3393 3476 3486 3596 3608 3677
Size: 421 1 564 83 10 110 12 69
Cuts at: 3677 3956 4076 2407
Size: 279 120 5256
Fragments arranged by size:
5256 564 421 279 120 110 83 69
12 10 1
EcoRI G'AATT_C
Cuts at: 4034 4234 4612 4034
Size: 200 378 6347
Fragments arranged by size:
6347 378 200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 4075 2401
Size: 1674 5251
FspI TGC'GCA
Cuts at: 5431 5431
Size: 6925
HaeII r_GCGC'y
Cuts at: 1678 2920 6072 1678
Size: 1242 3152 2531
Fragments arranged by size:
3152 2531 1242
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2411 2443 2462 2494 2572
Size: 32 135 32 839 32 19 32 78
Cuts at: 2572 2604 3287 3319 3425 3448 3457 3480 5566
Size: 32 683 32 106 23 9 23 2086
Cuts at: 5566 5598 5640 5672 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2086 839 683 135 106 78 42
32 32 32 32 32 32 32 32
23 23 19 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 4269 4515 669
Size: 988 281 268 2063 246 3079
Fragments arranged by size:
3079 2063 988 281 268 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6925
HpaI GTT'AAC
Cuts at: 2206 4515 2206
Size: 2309 4616
KpnI G_GTAC'C
Cuts at: 4250 4250
Size: 6925
MfeI C'AATT_G
Cuts at: 4524 4524
Size: 6925
MluI A'CGCG_T
Cuts at: 6801 6801
Size: 6925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3390 6039 6223 1305
Size: 2085 2649 184 2007
Fragments arranged by size:
2649 2085 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4169 10
Size: 54 1425 343 2337 2766
Fragments arranged by size:
2766 2337 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 4883 5242 5401 518
Size: 648 945 2772 359 159 2042
Fragments arranged by size:
2772 2042 945 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2216 4341 5018 6166 6411 1606
Size: 441 169 2125 677 1148 245 2120
Fragments arranged by size:
2125 2120 1148 677 441 245 169
NaeI GCC'GGC
Cuts at: 2025 3570 2025
Size: 1545 5380
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5434
Fragments arranged by size:
5434 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6925
NgoMIV G'CCGG_C
Cuts at: 2023 3568 2023
Size: 1545 5380
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6925
NotI GC'GGCC_GC
Cuts at: 4372 4372
Size: 6925
NspI r_CATG'y
Cuts at: 1132 1229 3442 4283 5828 1132
Size: 97 2213 841 1545 2229
Fragments arranged by size:
2229 2213 1545 841 97
PasI CC'CwG_GG
Cuts at: 1348 3197 4206 1348
Size: 1849 1009 4067
Fragments arranged by size:
4067 1849 1009
PciI A'CATG_T
Cuts at: 3438 5824 3438
Size: 2386 4539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2050 2572 3814 1108
Size: 942 522 1242 4219
Fragments arranged by size:
4219 1242 942 522
PfoI T'CCnGG_A
Cuts at: 2073 2871 2073
Size: 798 6127
PmeI GTTT'AAAC
Cuts at: 4229 4229
Size: 6925
PmlI CAC'GTG
Cuts at: 3275 3275
Size: 6925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4994 5026 6507 6539 4994
Size: 32 1481 32 5380
Fragments arranged by size:
5380 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2407 3476 3486 2407
Size: 1069 10 5846
Fragments arranged by size:
5846 1069 10
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6925
PsiI TTA'TAA
Cuts at: 4495 4495
Size: 6925
PspOMI G'GGCC_C
Cuts at: 2828 3393 3677 4075 2828
Size: 565 284 398 5678
Fragments arranged by size:
5678 565 398 284
PstI C_TGCA'G
Cuts at: 830 3180 3663 4277 830
Size: 2350 483 614 3478
Fragments arranged by size:
3478 2350 614 483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6925
SacI G_AGCT'C
Cuts at: 2172 3696 2172
Size: 1524 5401
SalI G'TCGA_C
Cuts at: 1936 4267 1936
Size: 2331 4594
SanDI GG'GwC_CC
Cuts at: 3486 3486
Size: 6925
SapI GCTCTTCn'nnn_
Cuts at: 2160 2716 3377 3916 2160
Size: 556 661 539 5169
Fragments arranged by size:
5169 661 556 539
SbfI CC_TGCA'GG
Cuts at: 4277 4277
Size: 6925
ScaI AGT'ACT
Cuts at: 5173 6885 5173
Size: 1712 5213
SfcI C'TryA_G
Cuts at: 826 1045 2159 3176 3659 4273 5408 6089 6280
Size: 219 1114 1017 483 614 1135 681 191
Cuts at: 6280 826
Size: 1471
Fragments arranged by size:
1471 1135 1114 1017 681 614 483 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6925
SmaI CCC'GGG
Cuts at: 1872 4252 1872
Size: 2380 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2519 2716 4083 5029 5930 6192
Size: 197 926 576 197 1367 946 901 262
Cuts at: 6192 820
Size: 1553
Fragments arranged by size:
1553 1367 946 926 901 576 262 197
197
SnaBI TAC'GTA
Cuts at: 493 4716 493
Size: 4223 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6925
SphI G_CATG'C
Cuts at: 1132 4283 1132
Size: 3151 3774
SspI AAT'ATT
Cuts at: 5 52 4849 5
Size: 47 4797 2081
Fragments arranged by size:
4797 2081 47
StuI AGG'CCT
Cuts at: 1517 3248 1517
Size: 1731 5194
StyI C'CwwG_G
Cuts at: 513 1065 2004 4193 4364 513
Size: 552 939 2189 171 3074
Fragments arranged by size:
3074 2189 939 552 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2094 2657 3959 4952 4969 5122 5307
Size: 1255 514 563 1302 993 17 153 185
Cuts at: 5307 5752 325
Size: 445 1498
Fragments arranged by size:
1498 1302 1255 993 563 514 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2103 5171 6883
Size: 274 80 33 51 1443 126 3068 1712
Cuts at: 6883 96
Size: 138
Fragments arranged by size:
3068 1712 1443 274 138 126 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2178 2210 2396 2428 3934 3966 6885 6917 2178
Size: 32 186 32 1506 32 2919 32 2186
Fragments arranged by size:
2919 2186 1506 186 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6925
XbaI T'CTAG_A
Cuts at: 4261 4261
Size: 6925
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4159 4159
Size: 6925
XhoI C'TCGA_G
Cuts at: 1943 4083 1943
Size: 2140 4785
XmaI C'CCGG_G
Cuts at: 1870 4250 1870
Size: 2380 4545
XmnI GAAnn'nnTTC
Cuts at: 2154 5054 2154
Size: 2900 4025
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4733 276
Size: 53 83 186 936 3199 2468
Fragments arranged by size:
3199 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PspXI
PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI
SwaI