Restriction map for
pFN21AA0178
(Circular) (Six-base) MAPSORT of: pFN21AA0178.seq Check: 6087 from: 1 to: 8404
pFN21AA0178 8404 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6214 278
Size: 53 83 186 936 4678 2468
Fragments arranged by size:
4678 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2056 2253 4017 5537 5747 1937
Size: 119 197 1764 1520 210 4594
Fragments arranged by size:
4594 1764 1520 210 197 119
Acc65I G'GTAC_C
Cuts at: 2628 5725 2628
Size: 3097 5307
AclI AA'CG_TT
Cuts at: 1563 6531 6904 1563
Size: 4968 373 3063
Fragments arranged by size:
4968 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2559 2660 2813 3788 4001 4508 4643 4994
Size: 927 101 153 975 213 507 135 351
Cuts at: 4994 5039 5334 6467 7851 8119 1632
Size: 45 295 1133 1384 268 1917
Fragments arranged by size:
1917 1384 1133 975 927 507 351 295
268 213 153 135 101 45
AfeI AGC'GCT
Cuts at: 4043 5201 4043
Size: 1158 7246
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8207
AflIII A'CryG_T
Cuts at: 1203 4512 7303 8280 1203
Size: 3309 2791 977 1327
Fragments arranged by size:
3309 2791 1327 977
AgeI A'CCGG_T
Cuts at: 2625 3132 7235 2625
Size: 507 4103 3794
Fragments arranged by size:
4103 3794 507
AhdI GACnn_n'nnGTC
Cuts at: 5386 7133 5386
Size: 1747 6657
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2840 1184
Size: 1656 6748
AlwNI CAG_nnn'CTG
Cuts at: 2638 3181 4636 5032 6147 7719 2638
Size: 543 1455 396 1115 1572 3323
Fragments arranged by size:
3323 1572 1455 1115 543 396
ApaI G_GGCC'C
Cuts at: 5303 5303
Size: 8404
ApaLI G'TGCA_C
Cuts at: 6461 7617 6461
Size: 1156 7248
ApoI r'AATT_y
Cuts at: 1493 3188 4908 5713 5939 6091 8200 1493
Size: 1695 1720 805 226 152 2109 1697
Fragments arranged by size:
2109 1720 1697 1695 805 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4248 6958 160
Size: 872 3216 2710 1606
Fragments arranged by size:
3216 2710 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8404
AvaI C'yCGr_G
Cuts at: 1870 1943 4697 5729 1870
Size: 73 2754 1032 4545
Fragments arranged by size:
4545 2754 1032 73
BamHI G'GATC_C
Cuts at: 5734 6101 5734
Size: 367 8037
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2628 4703 4838 5725 7180
Size: 325 217 627 841 2075 135 887 1455
Cuts at: 7180 618
Size: 1842
Fragments arranged by size:
2075 1842 1455 887 841 627 325 217
135
BanII G_rGCy'C
Cuts at: 1391 2209 2662 5303 5322 5723 1391
Size: 818 453 2641 19 401 4072
Fragments arranged by size:
4072 2641 818 453 401 19
BbeI G_GCGC'C
Cuts at: 4707 4707
Size: 8404
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3662 4451 5408 928
Size: 971 1763 789 957 3924
Fragments arranged by size:
3924 1763 971 957 789
BbvCI CC'TCA_GC
Cuts at: 2271 4989 2271
Size: 2718 5686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6595 6629 1903
Size: 34 4658 34 3678
Fragments arranged by size:
4658 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6296 7506 6296
Size: 1210 7194
BclI T'GATC_A
Cuts at: 1548 5637 1548
Size: 4089 4315
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2234 5174 7015
Size: 107 122 71 631 605 562 2940 1841
Cuts at: 7015 136
Size: 1525
Fragments arranged by size:
2940 1841 1525 631 605 562 122 107
71
BglII A'GATC_T
Cuts at: 5253 8399 5253
Size: 3146 5258
BlpI GC'TnA_GC
Cuts at: 4608 5821 4608
Size: 1213 7191
Bme1580I G_kGCm'C
Cuts at: 1790 5287 5303 6465 7621 1790
Size: 3497 16 1162 1156 2573
Fragments arranged by size:
3497 2573 1162 1156 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6135 7093 442
Size: 416 53 83 399 24 4718 958 1753
Fragments arranged by size:
4718 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4229 1056
Size: 3173 5231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2478 2884 2935 4888 4952 2478
Size: 406 51 1953 64 5930
Fragments arranged by size:
5930 1953 406 64 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3032 3203 3338 3752 4235 7064 1239
Size: 1793 171 135 414 483 2829 2579
Fragments arranged by size:
2829 2579 1793 483 414 171 135
Bpu10I CC'TnA_GC
Cuts at: 2271 2661 4989 5619 2271
Size: 390 2328 630 5056
Fragments arranged by size:
5056 2328 630 390
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4124 5555 6529 7394 7692 4124
Size: 1431 974 865 298 4836
Fragments arranged by size:
4836 1431 974 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3192 3701 4592 4745 5117 7067 882
Size: 617 1693 509 891 153 372 1950 2219
Fragments arranged by size:
2219 1950 1693 891 617 509 372 153
BsaAI yAC'GTr
Cuts at: 493 1204 6195 493
Size: 711 4991 2702
Fragments arranged by size:
4991 2702 711
BsaBI GATnn'nnATC
Cuts at: 8398 8398
Size: 8404
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4704 6211 6593 7228
Size: 53 83 186 936 3171 1507 382 635
Cuts at: 7228 275
Size: 1451
Fragments arranged by size:
3171 1507 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2625 3132 6837 7235 7509 7656 8067 2625
Size: 507 3705 398 274 147 411 2962
Fragments arranged by size:
3705 2962 507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2196 2226 3011 3041 1178
Size: 30 165 30 793 30 785 30 6541
Fragments arranged by size:
6541 793 785 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 3122 3401 4067 4694 5591 1186
Size: 1090 846 279 666 627 897 3999
Fragments arranged by size:
3999 1090 897 846 666 627 279
BseYI C'CCAG_C
Cuts at: 2812 3999 7607 8103 2812
Size: 1187 3608 496 3113
Fragments arranged by size:
3608 3113 1187 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4127 1163
Size: 573 2391 5440
Fragments arranged by size:
5440 2391 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3475 3556 5854 6615 7643 1540
Size: 275 186 1474 81 2298 761 1028 2301
Fragments arranged by size:
2301 2298 1474 1028 761 275 186 81
BsiHKAI G_wGCw'C
Cuts at: 2209 6465 6550 7621 2209
Size: 4256 85 1071 2992
Fragments arranged by size:
4256 2992 1071 85
BsmI GAATG_Cn'
Cuts at: 2379 2971 4458 5177 5291 5915 6008 2379
Size: 592 1487 719 114 624 93 4775
Fragments arranged by size:
4775 1487 719 624 592 114 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2209 2662 5287 5303 5322 5723 6465
Size: 399 419 453 2625 16 19 401 742
Cuts at: 6465 6550 7621 1391
Size: 85 1071 2174
Fragments arranged by size:
2625 2174 1071 742 453 419 401 399
85 19 16
BspEI T'CCGG_A
Cuts at: 8067 8067
Size: 8404
BspHI T'CATG_A
Cuts at: 4669 6293 8023 4669
Size: 1624 1730 5050
Fragments arranged by size:
5050 1730 1624
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 5692 5759 844
Size: 1334 3514 67 3489
Fragments arranged by size:
3514 3489 1334 67
BsrBI CCG'CTC
Cuts at: 1394 1823 6291 1394
Size: 429 4468 3507
Fragments arranged by size:
4468 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3853 3885 6899 7073 65
Size: 1183 2605 32 3014 174 1396
Fragments arranged by size:
3014 2605 1396 1183 174 32
BsrFI r'CCGG_y
Cuts at: 1700 1810 2625 3132 7048 7235 1700
Size: 110 815 507 3916 187 2869
Fragments arranged by size:
3916 2869 815 507 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8404
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8404
BssSI C'ACGA_G
Cuts at: 4926 6464 7476 4926
Size: 1538 1012 5854
Fragments arranged by size:
5854 1538 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3277 5050 794
Size: 2483 1773 4148
Fragments arranged by size:
4148 2483 1773
BstBI TT'CG_AA
Cuts at: 5181 7208 5181
Size: 2027 6377
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8404
BstXI CCAn_nnnn'nTGG
Cuts at: 3616 3643 3616
Size: 27 8377
BstYI r'GATC_y
Cuts at: 1972 3226 4969 5253 5734 6101 6486 6503 7955
Size: 1254 1743 284 481 367 385 17 1452
Cuts at: 7955 8041 8053 8399 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1743 1452 1254 481 385 367 346
284 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2200 4120 4602 4842 5085 513
Size: 552 939 196 1920 482 240 243 3832
Fragments arranged by size:
3832 1920 939 552 482 243 240 196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2549 487
Size: 683 56 1323 6342
Fragments arranged by size:
6342 1323 683 56
BtsI GCAGTG_nn'
Cuts at: 4780 5018 5287 5920 6714 6734 4780
Size: 238 269 633 794 20 6450
Fragments arranged by size:
6450 794 633 269 238 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8369
DraI TTT'AAA
Cuts at: 5708 5708
Size: 8404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7411 809
Size: 507 6095 1802
Fragments arranged by size:
6095 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3399 3553 4638
Size: 54 1425 50 275 18 1569 154 1085
Cuts at: 4638 5851 6740 8369 8
Size: 1213 889 1629 43
Fragments arranged by size:
1629 1569 1425 1213 1085 889 275 154
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 5851 1537
Size: 275 1741 2298 4090
Fragments arranged by size:
4090 2298 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2466 2535 2610 3066 3285 3348 4332 4857
Size: 1113 69 75 456 219 63 984 525
Cuts at: 4857 4989 5310 6334 1353
Size: 132 321 1024 3423
Fragments arranged by size:
3423 1113 1024 984 525 456 321 219
132 75 69 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6991 7365 7511 387
Size: 1064 138 5402 374 146 1280
Fragments arranged by size:
5402 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2207 2207
Size: 8404
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2559 2660 2813 3032 3203 3338 3752
Size: 393 927 101 153 219 171 135 414
Cuts at: 3752 3788 4001 4235 4508 4643 4994 5039 5334
Size: 36 213 234 273 135 351 45 295
Cuts at: 5334 6467 7064 7851 8119 1239
Size: 1133 597 787 268 1524
Fragments arranged by size:
1524 1133 927 787 597 414 393 351
295 273 268 234 219 213 171 153
135 135 101 45 36
EcoNI CCTnn'n_nnAGG
Cuts at: 3173 5622 3173
Size: 2449 5955
EcoO109I rG'GnC_Cy
Cuts at: 2191 3826 4933 5263 5300 2191
Size: 1635 1107 330 37 5295
Fragments arranged by size:
5295 1635 1107 330 37
EcoRI G'AATT_C
Cuts at: 5713 6091 5713
Size: 378 8026
EcoRV GAT'ATC
Cuts at: 4255 4255
Size: 8404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4060 4468 5218 4060
Size: 408 750 7246
Fragments arranged by size:
7246 750 408
FspI TGC'GCA
Cuts at: 4364 6910 4364
Size: 2546 5858
HaeII r_GCGC'y
Cuts at: 1678 2638 3532 3910 4000 4045 4707 5203 7551
Size: 960 894 378 90 45 662 496 2348
Cuts at: 7551 1678
Size: 2531
Fragments arranged by size:
2531 2348 960 894 662 496 378 90
45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2049 2081 3011 3043 3067
Size: 32 135 32 477 32 930 32 24
Cuts at: 3067 3099 4165 4197 4591 4623 5372 5404 7045
Size: 32 1066 32 394 32 749 32 1641
Cuts at: 7045 7077 7119 7151 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1641 1066 930 749 477 394 135
42 32 32 32 32 32 32 32
32 32 32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 2057 5538 5748 5994 669
Size: 988 281 119 3481 210 246 3079
Fragments arranged by size:
3481 3079 988 281 246 210 119
HindIII A'AGCT_T
Cuts at: 748 2684 4757 748
Size: 1936 2073 4395
Fragments arranged by size:
4395 2073 1936
HpaI GTT'AAC
Cuts at: 5994 5994
Size: 8404
KasI G'GCGC_C
Cuts at: 4703 4703
Size: 8404
KpnI G_GTAC'C
Cuts at: 2632 5729 2632
Size: 3097 5307
MfeI C'AATT_G
Cuts at: 6003 6003
Size: 8404
MluI A'CGCG_T
Cuts at: 8280 8280
Size: 8404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2365 3084 4446 4629 7518 7702 1305
Size: 1060 719 1362 183 2889 184 2007
Fragments arranged by size:
2889 2007 1362 1060 719 184 183
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3401 4640 10
Size: 54 1425 343 1569 1239 3774
Fragments arranged by size:
3774 1569 1425 1239 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3641 6362 6721 6880 518
Size: 648 2475 2721 359 159 2042
Fragments arranged by size:
2721 2475 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2225 2671 5820 6497 7645 7890 1606
Size: 619 446 3149 677 1148 245 2120
Fragments arranged by size:
3149 2120 1148 677 619 446 245
NarI GG'CG_CC
Cuts at: 4704 4704
Size: 8404
NcoI C'CATG_G
Cuts at: 513 1065 2004 2200 4120 4602 4842 5085 513
Size: 552 939 196 1920 482 240 243 3832
Fragments arranged by size:
3832 1920 939 552 482 243 240 196
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8404
NheI G'CTAG_C
Cuts at: 1052 4225 1052
Size: 3173 5231
NotI GC'GGCC_GC
Cuts at: 5851 5851
Size: 8404
NspI r_CATG'y
Cuts at: 1132 1229 4516 5762 7307 1132
Size: 97 3287 1246 1545 2229
Fragments arranged by size:
3287 2229 1545 1246 97
PasI CC'CwG_GG
Cuts at: 1348 4876 1348
Size: 3528 4876
PciI A'CATG_T
Cuts at: 4512 7303 4512
Size: 2791 5613
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3181 3445 1108
Size: 2073 264 6067
Fragments arranged by size:
6067 2073 264
PfoI T'CCnGG_A
Cuts at: 3759 4371 5118 5466 3759
Size: 612 747 348 6697
Fragments arranged by size:
6697 747 612 348
PmeI GTTT'AAAC
Cuts at: 5708 5708
Size: 8404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6473 6505 7986 8018 6473
Size: 32 1481 32 6859
Fragments arranged by size:
6859 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2191
Size: 8404
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8404
PsiI TTA'TAA
Cuts at: 5974 5974
Size: 8404
PspOMI G'GGCC_C
Cuts at: 5299 5299
Size: 8404
PstI C_TGCA'G
Cuts at: 830 2676 2964 3834 4131 4200 5025 5756 830
Size: 1846 288 870 297 69 825 731 3478
Fragments arranged by size:
3478 1846 870 825 731 297 288 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8404
PvuII CAG'CTG
Cuts at: 2225 2671 2225
Size: 446 7958
SacI G_AGCT'C
Cuts at: 2209 2209
Size: 8404
SalI G'TCGA_C
Cuts at: 1936 2055 5536 5746 1936
Size: 119 3481 210 4594
Fragments arranged by size:
4594 3481 210 119
SapI GCTCTTCn'nnn_
Cuts at: 3348 5310 3348
Size: 1962 6442
SbfI CC_TGCA'GG
Cuts at: 5756 5756
Size: 8404
ScaI AGT'ACT
Cuts at: 4145 6652 8364 4145
Size: 2507 1712 4185
Fragments arranged by size:
4185 2507 1712
SexAI A'CCwGG_T
Cuts at: 3438 4314 3438
Size: 876 7528
SfcI C'TryA_G
Cuts at: 826 1045 2672 2960 3744 3830 4127 4196 4793
Size: 219 1627 288 784 86 297 69 597
Cuts at: 4793 5021 5752 6887 7568 7759 826
Size: 228 731 1135 681 191 1471
Fragments arranged by size:
1627 1471 1135 784 731 681 597 297
288 228 219 191 86 69
SfoI GGC'GCC
Cuts at: 4705 4705
Size: 8404
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8404
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8404
SmaI CCC'GGG
Cuts at: 1872 5731 1872
Size: 3859 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4139 5570 6508 7409 7671 820
Size: 197 926 2196 1431 938 901 262 1553
Fragments arranged by size:
2196 1553 1431 938 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6195 493
Size: 5702 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8404
SphI G_CATG'C
Cuts at: 1132 5762 1132
Size: 4630 3774
SspI AAT'ATT
Cuts at: 5 52 2578 6328 5
Size: 47 2526 3750 2081
Fragments arranged by size:
3750 2526 2081 47
StuI AGG'CCT
Cuts at: 1517 4799 1517
Size: 3282 5122
StyI C'CwwG_G
Cuts at: 513 1065 2004 2200 2907 3609 3987 4120 4602
Size: 552 939 196 707 702 378 133 482
Cuts at: 4602 4842 5085 5487 5589 5843 513
Size: 240 243 402 102 254 3074
Fragments arranged by size:
3074 939 707 702 552 482 402 378
254 243 240 196 133 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6431 6448 6601 6786 7231 325
Size: 1255 4851 17 153 185 445 1498
Fragments arranged by size:
4851 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2316 2352 2895
Size: 274 80 33 51 1443 339 36 543
Cuts at: 2895 4143 4507 6650 8362 96
Size: 1248 364 2143 1712 138
Fragments arranged by size:
2143 1712 1443 1248 543 364 339 274
138 80 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2196 2228 8364 8396 2196
Size: 32 6136 32 2204
Fragments arranged by size:
6136 2204 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8404
XbaI T'CTAG_A
Cuts at: 5740 5740
Size: 8404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4423 5092 4423
Size: 669 7735
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8404
XmaI C'CCGG_G
Cuts at: 1870 5729 1870
Size: 3859 4545
XmnI GAAnn'nnTTC
Cuts at: 5366 6533 5366
Size: 1167 7237
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6212 276
Size: 53 83 186 936 4678 2468
Fragments arranged by size:
4678 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SfiI SrfI SwaI