Restriction map for
pFN21AA0166
(Circular) (Six-base) MAPSORT of: pFN21AA0166.seq Check: 7522 from: 1 to: 11332
pFN21AA0166 11332 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9142 278
Size: 53 83 186 936 7606 2468
Fragments arranged by size:
7606 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2541 3369 5702 6914 8675 1937
Size: 604 828 2333 1212 1761 4594
Fragments arranged by size:
4594 2333 1761 1212 828 604
Acc65I G'GTAC_C
Cuts at: 2052 2450 5843 8653 2052
Size: 398 3393 2810 4731
Fragments arranged by size:
4731 3393 2810 398
AclI AA'CG_TT
Cuts at: 1563 9459 9832 1563
Size: 7896 373 3063
Fragments arranged by size:
7896 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2280 2489 2530 2961 3162 3255 3876 4788
Size: 648 209 41 431 201 93 621 912
Cuts at: 4788 5578 5765 5774 5837 6374 6965 6980 7122
Size: 790 187 9 63 537 591 15 142
Cuts at: 7122 7658 8029 8517 8627 9395 10779 11047 1632
Size: 536 371 488 110 768 1384 268 1917
Fragments arranged by size:
1917 1384 912 790 768 648 621 591
537 536 488 431 371 268 209 201
187 142 110 93 63 41 15 9
AflII C'TTAA_G
Cuts at: 820 1017 2167 4892 6191 8515 820
Size: 197 1150 2725 1299 2324 3637
Fragments arranged by size:
3637 2725 2324 1299 1150 197
AflIII A'CryG_T
Cuts at: 1203 5321 6739 6909 6925 7455 10231 11208 1203
Size: 4118 1418 170 16 530 2776 977 1327
Fragments arranged by size:
4118 2776 1418 1327 977 530 170 16
AgeI A'CCGG_T
Cuts at: 2678 10163 2678
Size: 7485 3847
AhdI GACnn_n'nnGTC
Cuts at: 10061 10061
Size: 11332
AleI CACnn'nnGTG
Cuts at: 1166 4160 1166
Size: 2994 8338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 11332
AlwNI CAG_nnn'CTG
Cuts at: 3155 4471 5173 5372 7253 8588 9075 10647 3155
Size: 1316 702 199 1881 1335 487 1572 3840
Fragments arranged by size:
3840 1881 1572 1335 1316 702 487 199
ApaLI G'TGCA_C
Cuts at: 3693 9389 10545 3693
Size: 5696 1156 4480
Fragments arranged by size:
5696 4480 1156
ApoI r'AATT_y
Cuts at: 1493 2517 3410 3602 3626 5761 6696 6822 7130
Size: 1024 893 192 24 2135 935 126 308
Cuts at: 7130 7359 7366 8284 8601 8641 8867 9019 11128
Size: 229 7 918 317 40 226 152 2109
Cuts at: 11128 1493
Size: 1697
Fragments arranged by size:
2135 2109 1697 1024 935 918 893 317
308 229 226 192 152 126 40 24
7
AseI AT'TA_AT
Cuts at: 160 1032 2960 6645 6651 6881 7434 9886 160
Size: 872 1928 3685 6 230 553 2452 1606
Fragments arranged by size:
3685 2452 1928 1606 872 553 230 6
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11332
AvaI C'yCGr_G
Cuts at: 1870 1943 7665 8657 1870
Size: 73 5722 992 4545
Fragments arranged by size:
5722 4545 992 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2044 2077 2247 2280 2044
Size: 33 170 33 11096
Fragments arranged by size:
11096 170 33 33
BamHI G'GATC_C
Cuts at: 6571 8662 9029 6571
Size: 2091 367 8874
Fragments arranged by size:
8874 2091 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2052 2450 5843 8653 10108
Size: 325 217 627 265 398 3393 2810 1455
Cuts at: 10108 618
Size: 1842
Fragments arranged by size:
3393 2810 1842 1455 627 398 325 265
217
BanII G_rGCy'C
Cuts at: 1391 3559 5118 5141 6152 7672 8651 1391
Size: 2168 1559 23 1011 1520 979 4072
Fragments arranged by size:
4072 2168 1559 1520 1011 979 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3150 4737 4880 5843 928
Size: 971 1251 1587 143 963 6417
Fragments arranged by size:
6417 1587 1251 971 963 143
BbvCI CC'TCA_GC
Cuts at: 5127 5127
Size: 11332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 9523 9557 1903
Size: 34 7586 34 3678
Fragments arranged by size:
7586 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8027 9224 10434 8027
Size: 1197 1210 8925
Fragments arranged by size:
8925 1210 1197
BclI T'GATC_A
Cuts at: 1548 4089 8110 1548
Size: 2541 4021 4770
Fragments arranged by size:
4770 4021 2541
BfrBI ATG'CAT
Cuts at: 2372 7639 8461 2372
Size: 5267 822 5243
Fragments arranged by size:
5267 5243 822
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9943 136
Size: 107 122 71 631 605 8271 1525
Fragments arranged by size:
8271 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2118 4683 5044 6025 11327 2118
Size: 2565 361 981 5302 2123
Fragments arranged by size:
5302 2565 2123 981 361
BlpI GC'TnA_GC
Cuts at: 8749 8749
Size: 11332
Bme1580I G_kGCm'C
Cuts at: 1790 3697 4807 7247 9393 10549 1790
Size: 1907 1110 2440 2146 1156 2573
Fragments arranged by size:
2573 2440 2146 1907 1156 1110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11332
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5310 5354 6214
Size: 416 53 83 399 24 3893 44 860
Cuts at: 6214 9063 10021 442
Size: 2849 958 1753
Fragments arranged by size:
3893 2849 1753 958 860 416 399 83
53 44 24
BmtI G_CTAG'C
Cuts at: 1056 4980 5816 8357 1056
Size: 3924 836 2541 4031
Fragments arranged by size:
4031 3924 2541 836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2658 3776 4876 5222 7996 8565 9992 1239
Size: 1419 1118 1100 346 2774 569 1427 2579
Fragments arranged by size:
2774 2579 1427 1419 1118 1100 569 346
Bpu10I CC'TnA_GC
Cuts at: 3378 4058 5127 3378
Size: 680 1069 9583
Fragments arranged by size:
9583 1069 680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3447 4391 5239 5636 6521 9457 10322 10620 3447
Size: 944 848 397 885 2936 865 298 4159
Fragments arranged by size:
4159 2936 944 885 865 848 397 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 9995 882
Size: 617 8496 2219
Fragments arranged by size:
8496 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2884 2934 6910 9123 493
Size: 711 1680 50 3976 2213 2702
Fragments arranged by size:
3976 2702 2213 1680 711 50
BsaBI GATnn'nnATC
Cuts at: 2397 3997 11326 2397
Size: 1600 7329 2403
Fragments arranged by size:
7329 2403 1600
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 9139 9521 10156 275
Size: 53 83 186 936 7606 382 635 1451
Fragments arranged by size:
7606 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2042 2678 9765 10163 10437 10584 10995 2042
Size: 636 7087 398 274 147 411 2379
Fragments arranged by size:
7087 2379 636 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2839 2869 1178
Size: 30 165 30 1436 30 9641
Fragments arranged by size:
9641 1436 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4274 5149 6693 7550 1186
Size: 3088 875 1544 857 4968
Fragments arranged by size:
4968 3088 1544 875 857
BseYI C'CCAG_C
Cuts at: 10535 11031 10535
Size: 496 10836
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4461 5144 5981 6034 7571 1163
Size: 573 2725 683 837 53 1537 4924
Fragments arranged by size:
4924 2725 1537 837 683 573 53
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7241 8782 9543 10571 1540
Size: 275 186 5240 1541 761 1028 2301
Fragments arranged by size:
5240 2301 1541 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3559 3697 4407 5108 6527 7260 7672 9393 9478
Size: 138 710 701 1419 733 412 1721 85
Cuts at: 9478 10549 3559
Size: 1071 4342
Fragments arranged by size:
4342 1721 1419 1071 733 710 701 412
138 85
BsmI GAATG_Cn'
Cuts at: 2690 5884 6228 7599 8843 8936 2690
Size: 3194 344 1371 1244 93 5086
Fragments arranged by size:
5086 3194 1371 1244 344 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3559 3697 4407 4807 5108 5118 5141
Size: 399 1769 138 710 400 301 10 23
Cuts at: 5141 6152 6527 7247 7260 7672 8651 9393 9478
Size: 1011 375 720 13 412 979 742 85
Cuts at: 9478 10549 1391
Size: 1071 2174
Fragments arranged by size:
2174 1769 1071 1011 979 742 720 710
412 400 399 375 301 138 85 23
13 10
BspEI T'CCGG_A
Cuts at: 10995 10995
Size: 11332
BspHI T'CATG_A
Cuts at: 5532 5874 9221 10951 5532
Size: 342 3347 1730 5913
Fragments arranged by size:
5913 3347 1730 342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 7938 8023 8687 844
Size: 7094 85 664 3489
Fragments arranged by size:
7094 3489 664 85
BsrBI CCG'CTC
Cuts at: 1394 1823 9219 1394
Size: 429 7396 3507
Fragments arranged by size:
7396 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5296 6860 9827 10001 65
Size: 1183 4048 1564 2967 174 1396
Fragments arranged by size:
4048 2967 1564 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2678 6428 7233 9976 10163 1700
Size: 110 868 3750 805 2743 187 2869
Fragments arranged by size:
3750 2869 2743 868 805 187 110
BsrGI T'GTAC_A
Cuts at: 96 4278 96
Size: 4182 7150
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11332
BssSI C'ACGA_G
Cuts at: 7871 9392 10404 7871
Size: 1521 1012 8799
Fragments arranged by size:
8799 1521 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 11332
BstBI TT'CG_AA
Cuts at: 6875 6933 10136 6875
Size: 58 3203 8071
Fragments arranged by size:
8071 3203 58
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11332
BstXI CCAn_nnnn'nTGG
Cuts at: 6487 6487
Size: 11332
BstYI r'GATC_y
Cuts at: 1972 2118 3396 4683 5044 5560 6025 6571 6712
Size: 146 1278 1287 361 516 465 546 141
Cuts at: 6712 8662 9029 9414 9431 10883 10969 10981 11327
Size: 1950 367 385 17 1452 86 12 346
Cuts at: 11327 1972
Size: 1977
Fragments arranged by size:
1977 1950 1452 1287 1278 546 516 465
385 367 361 346 146 141 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 8307 8307
Size: 11332
BtgI C'CryG_G
Cuts at: 513 1065 3809 4353 4881 6708 7878 513
Size: 552 2744 544 528 1827 1170 3967
Fragments arranged by size:
3967 2744 1827 1170 552 544 528
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6393 7158 8125 8480 487
Size: 683 56 5167 765 967 355 3339
Fragments arranged by size:
5167 3339 967 765 683 355 56
BtsI GCAGTG_nn'
Cuts at: 4431 5284 8078 8560 8586 8848 9642 9662 4431
Size: 853 2794 482 26 262 794 20 6101
Fragments arranged by size:
6101 2794 853 794 482 262 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11332
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11297
DraI TTT'AAA
Cuts at: 2514 3817 4647 4966 7723 8001 8319 8636 2514
Size: 1303 830 319 2757 278 318 317 5210
Fragments arranged by size:
5210 2757 1303 830 319 318 317 278
DraIII CAC_nnn'GTG
Cuts at: 5863 5863
Size: 11332
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10339 809
Size: 507 9023 1802
Fragments arranged by size:
9023 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3929 6430 6489
Size: 54 1425 50 275 18 2099 2501 59
Cuts at: 6489 8779 9668 11297 8
Size: 2290 889 1629 43
Fragments arranged by size:
2501 2290 2099 1629 1425 889 275 59
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8779 1537
Size: 275 6967 4090
Fragments arranged by size:
6967 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2128 2883 4818 5565 6401 7540 7677 7688
Size: 775 755 1935 747 836 1139 137 11
Cuts at: 7688 8325 9262 1353
Size: 637 937 3423
Fragments arranged by size:
3423 1935 1139 937 836 775 755 747
637 137 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2419 9919 10293 10439 387
Size: 1064 138 830 7500 374 146 1280
Fragments arranged by size:
7500 1280 1064 830 374 146 138
EcoICRI GAG'CTC
Cuts at: 3557 7670 3557
Size: 4113 7219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2280 2489 2530 2658 2961 3162 3255
Size: 393 648 209 41 128 303 201 93
Cuts at: 3255 3776 3876 4788 4876 5222 5578 5765 5774
Size: 521 100 912 88 346 356 187 9
Cuts at: 5774 5837 6374 6965 6980 7122 7658 7996 8029
Size: 63 537 591 15 142 536 338 33
Cuts at: 8029 8517 8565 8627 9395 9992 10779 11047 1239
Size: 488 48 62 768 597 787 268 1524
Fragments arranged by size:
1524 912 787 768 648 597 591 537
536 521 488 393 356 346 338 303
268 209 201 187 142 128 100 93
88 63 62 48 41 33 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 7088 7833 7088
Size: 745 10587
EcoRI G'AATT_C
Cuts at: 7359 7366 8641 9019 7359
Size: 7 1275 378 9672
Fragments arranged by size:
9672 1275 378 7
EcoRV GAT'ATC
Cuts at: 5690 7096 7119 5690
Size: 1406 23 9903
Fragments arranged by size:
9903 1406 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2912 3665 3701 2912
Size: 753 36 10543
Fragments arranged by size:
10543 753 36
FspI TGC'GCA
Cuts at: 9838 9838
Size: 11332
HaeII r_GCGC'y
Cuts at: 1678 10479 1678
Size: 8801 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2779 2811 3137 3169 4074
Size: 32 135 32 1207 32 326 32 905
Cuts at: 4074 4106 4593 4610 4625 4642 4675 4707 5389
Size: 32 487 17 15 17 33 32 682
Cuts at: 5389 5421 5644 5676 7834 7866 7969 8001 8102
Size: 32 223 32 2158 32 103 32 101
Cuts at: 8102 8134 9973 10005 10047 10079 1373
Size: 32 1839 32 42 32 2626
Fragments arranged by size:
2626 2158 1839 1207 905 682 487 326
223 135 103 101 42 33 32 32
32 32 32 32 32 32 32 32
32 32 32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 6966 7051 7647 8676 8922 669
Size: 988 281 5028 85 596 1029 246 3079
Fragments arranged by size:
5028 3079 1029 988 596 281 246 85
HindIII A'AGCT_T
Cuts at: 748 3183 5330 5538 7217 7958 8529 748
Size: 2435 2147 208 1679 741 571 3551
Fragments arranged by size:
3551 2435 2147 1679 741 571 208
HpaI GTT'AAC
Cuts at: 6966 7647 8922 6966
Size: 681 1275 9376
Fragments arranged by size:
9376 1275 681
KpnI G_GTAC'C
Cuts at: 2056 2454 5847 8657 2056
Size: 398 3393 2810 4731
Fragments arranged by size:
4731 3393 2810 398
MfeI C'AATT_G
Cuts at: 2237 2529 3947 7575 8324 8931 2237
Size: 292 1418 3628 749 607 4638
Fragments arranged by size:
4638 3628 1418 749 607 292
MluI A'CGCG_T
Cuts at: 11208 11208
Size: 11332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3864 6351 6714 7443 10446 10630 1305
Size: 2559 2487 363 729 3003 184 2007
Fragments arranged by size:
3003 2559 2487 2007 729 363 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3931 6491 10
Size: 54 1425 343 2099 2560 4851
Fragments arranged by size:
4851 2560 2099 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4160 5289 9290 9649 9808 518
Size: 648 2994 1129 4001 359 159 2042
Fragments arranged by size:
4001 2994 2042 1129 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2785 4445 6761 8116 8748 9425 10573 10818
Size: 1179 1660 2316 1355 632 677 1148 245
Cuts at: 10818 1606
Size: 2120
Fragments arranged by size:
2316 2120 1660 1355 1179 1148 677 632
245
NaeI GCC'GGC
Cuts at: 6430 6430
Size: 11332
NcoI C'CATG_G
Cuts at: 513 1065 3809 4353 7878 513
Size: 552 2744 544 3525 3967
Fragments arranged by size:
3967 3525 2744 552 544
NdeI CA'TA_TG
Cuts at: 387 4799 5566 7807 8337 387
Size: 4412 767 2241 530 3382
Fragments arranged by size:
4412 3382 2241 767 530
NgoMIV G'CCGG_C
Cuts at: 6428 6428
Size: 11332
NheI G'CTAG_C
Cuts at: 1052 4976 5812 8353 1052
Size: 3924 836 2541 4031
Fragments arranged by size:
4031 3924 2541 836
NotI GC'GGCC_GC
Cuts at: 8779 8779
Size: 11332
NsiI A_TGCA'T
Cuts at: 2374 7641 8463 2374
Size: 5267 822 5243
Fragments arranged by size:
5267 5243 822
NspI r_CATG'y
Cuts at: 1132 1229 2163 5325 5936 6743 7459 8465 8690
Size: 97 934 3162 611 807 716 1006 225
Cuts at: 8690 10235 1132
Size: 1545 2229
Fragments arranged by size:
3162 2229 1545 1006 934 807 716 611
225 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 11332
PciI A'CATG_T
Cuts at: 5321 6739 7455 10231 5321
Size: 1418 716 2776 6422
Fragments arranged by size:
6422 2776 1418 716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3938 1108
Size: 2830 8502
PfoI T'CCnGG_A
Cuts at: 3399 3399
Size: 11332
PmeI GTTT'AAAC
Cuts at: 8636 8636
Size: 11332
PmlI CAC'GTG
Cuts at: 2934 2934
Size: 11332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 2082 2858 2890 7654 7686 9401 9433 10914
Size: 32 776 32 4764 32 1715 32 1481
Cuts at: 10914 10946 2050
Size: 32 2436
Fragments arranged by size:
4764 2436 1715 1481 776 32 32 32
32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11332
PsiI TTA'TAA
Cuts at: 4226 5759 8902 4226
Size: 1533 3143 6656
Fragments arranged by size:
6656 3143 1533
PspXI vC'TCGA_Gb
Cuts at: 7665 7665
Size: 11332
PstI C_TGCA'G
Cuts at: 830 4786 4929 5184 7935 8684 830
Size: 3956 143 255 2751 749 3478
Fragments arranged by size:
3956 3478 2751 749 255 143
PvuI CG_AT'CG
Cuts at: 2001 7241 2001
Size: 5240 6092
PvuII CAG'CTG
Cuts at: 2785 4445 6761 8116 2785
Size: 1660 2316 1355 6001
Fragments arranged by size:
6001 2316 1660 1355
SacI G_AGCT'C
Cuts at: 3559 7672 3559
Size: 4113 7219
SalI G'TCGA_C
Cuts at: 1936 8674 1936
Size: 6738 4594
SapI GCTCTTCn'nnn_
Cuts at: 4818 6401 7677 8325 4818
Size: 1583 1276 648 7825
Fragments arranged by size:
7825 1583 1276 648
SbfI CC_TGCA'GG
Cuts at: 8684 8684
Size: 11332
ScaI AGT'ACT
Cuts at: 3165 6211 9580 11292 3165
Size: 3046 3369 1712 3205
Fragments arranged by size:
3369 3205 3046 1712
SexAI A'CCwGG_T
Cuts at: 7492 7492
Size: 11332
SfcI C'TryA_G
Cuts at: 826 1045 2904 4687 4782 4866 4925 5180 5487
Size: 219 1859 1783 95 84 59 255 307
Cuts at: 5487 6831 6915 7044 7065 7160 7707 7931 8680
Size: 1344 84 129 21 95 547 224 749
Cuts at: 8680 9815 10496 10687 826
Size: 1135 681 191 1471
Fragments arranged by size:
1859 1783 1471 1344 1135 749 681 547
307 255 224 219 191 129 95 95
84 84 59 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11332
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11332
SmaI CCC'GGG
Cuts at: 1872 8659 1872
Size: 6787 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2167 3462 4406 4892 5218 5615
Size: 197 926 224 1295 944 486 326 397
Cuts at: 5615 6191 6500 7665 8515 9436 10337 10599 820
Size: 576 309 1165 850 921 901 262 1553
Fragments arranged by size:
1553 1295 1165 944 926 921 901 850
576 486 397 326 309 262 224 197
SnaBI TAC'GTA
Cuts at: 493 9123 493
Size: 8630 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11332
SphI G_CATG'C
Cuts at: 1132 2163 8465 8690 1132
Size: 1031 6302 225 3774
Fragments arranged by size:
6302 3774 1031 225
SspI AAT'ATT
Cuts at: 5 52 6353 6643 6649 7289 8552 9256 5
Size: 47 6301 290 6 640 1263 704 2081
Fragments arranged by size:
6301 2081 1263 704 640 290 47 6
StuI AGG'CCT
Cuts at: 1517 2135 4853 1517
Size: 618 2718 7996
Fragments arranged by size:
7996 2718 618
StyI C'CwwG_G
Cuts at: 513 1065 3809 4353 4433 5694 6433 7878 8771
Size: 552 2744 544 80 1261 739 1445 893
Cuts at: 8771 513
Size: 3074
Fragments arranged by size:
3074 2744 1445 1261 893 739 552 544
80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3365 9359 9376 9529 9714 10159 325
Size: 1255 1785 5994 17 153 185 445 1498
Fragments arranged by size:
5994 1785 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2549 3163 4107
Size: 274 80 33 51 1443 572 614 944
Cuts at: 4107 4278 6209 9578 11290 96
Size: 171 1931 3369 1712 138
Fragments arranged by size:
3369 1931 1712 1443 944 614 572 274
171 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2839 2871 7369 7401 11292 11324 2839
Size: 32 4498 32 3891 32 2847
Fragments arranged by size:
4498 3891 2847 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3149 1316
Size: 1833 9499
XbaI T'CTAG_A
Cuts at: 6715 8668 6715
Size: 1953 9379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5197 5571 5197
Size: 374 10958
XhoI C'TCGA_G
Cuts at: 1943 7665 1943
Size: 5722 5610
XmaI C'CCGG_G
Cuts at: 1870 8657 1870
Size: 6787 4545
XmnI GAAnn'nnTTC
Cuts at: 2892 6697 9461 2892
Size: 3805 2764 4763
Fragments arranged by size:
4763 3805 2764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9140 276
Size: 53 83 186 936 7606 2468
Fragments arranged by size:
7606 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BplI
BsiWI BsmBI BstZ17I EcoO109I FseI FspAI KasI NarI
NruI PacI PpuMI PspOMI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI