Restriction map for
pFN21AA0162
(Circular) (Six-base) MAPSORT of: pFN21AA0162.seq Check: 5206 from: 1 to: 9883
pFN21AA0162 9883 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3805 4282 6610 3805
Size: 477 2328 7078
Fragments arranged by size:
7078 2328 477
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7693 278
Size: 53 83 186 936 6157 2468
Fragments arranged by size:
6157 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3393 7226 1937
Size: 1456 3833 4594
Fragments arranged by size:
4594 3833 1456
Acc65I G'GTAC_C
Cuts at: 2071 6489 7204 2071
Size: 4418 715 4750
Fragments arranged by size:
4750 4418 715
AclI AA'CG_TT
Cuts at: 1563 8010 8383 1563
Size: 6447 373 3063
Fragments arranged by size:
6447 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2949 3031 3491 4002 4230 5027 5036 5940
Size: 1317 82 460 511 228 797 9 904
Cuts at: 5940 6163 7946 9330 9598 1632
Size: 223 1783 1384 268 1917
Fragments arranged by size:
1917 1783 1384 1317 904 797 511 460
268 228 223 82 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9686
AflIII A'CryG_T
Cuts at: 1203 3570 8782 9759 1203
Size: 2367 5212 977 1327
Fragments arranged by size:
5212 2367 1327 977
AgeI A'CCGG_T
Cuts at: 8714 8714
Size: 9883
AhdI GACnn_n'nnGTC
Cuts at: 4742 8612 4742
Size: 3870 6013
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9883
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6422 1184
Size: 5238 4645
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5775 5807 5775
Size: 32 9851
AlwNI CAG_nnn'CTG
Cuts at: 2581 3752 3820 4633 7048 7090 7626 9198 2581
Size: 1171 68 813 2415 42 536 1572 3266
Fragments arranged by size:
3266 2415 1572 1171 813 536 68 42
ApaI G_GGCC'C
Cuts at: 3063 3063
Size: 9883
ApaLI G'TGCA_C
Cuts at: 7940 9096 7940
Size: 1156 8727
ApoI r'AATT_y
Cuts at: 1493 2090 2323 2678 4517 4781 4886 5732 6215
Size: 597 233 355 1839 264 105 846 483
Cuts at: 6215 7192 7418 7570 9679 1493
Size: 977 226 152 2109 1697
Fragments arranged by size:
2109 1839 1697 977 846 597 483 355
264 233 226 152 105
AseI AT'TA_AT
Cuts at: 160 1032 8437 160
Size: 872 7405 1606
Fragments arranged by size:
7405 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9883
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 3632 5036 6273 6438 7208 1870
Size: 73 133 1556 1404 1237 165 770 4545
Fragments arranged by size:
4545 1556 1404 1237 770 165 133 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2339 2372 2339
Size: 33 9850
BamHI G'GATC_C
Cuts at: 3225 6553 7213 7580 3225
Size: 3328 660 367 5528
Fragments arranged by size:
5528 3328 660 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2071 3121 3764 4270 6489
Size: 325 217 627 284 1050 643 506 2219
Cuts at: 6489 7204 8659 618
Size: 715 1455 1842
Fragments arranged by size:
2219 1842 1455 1050 715 643 627 506
325 284 217
BanII G_rGCy'C
Cuts at: 1391 2826 3063 3331 3549 3702 3804 3996 5269
Size: 1435 237 268 218 153 102 192 1273
Cuts at: 5269 7202 1391
Size: 1933 4072
Fragments arranged by size:
4072 1933 1435 1273 268 237 218 192
153 102
BbeI G_GCGC'C
Cuts at: 3768 3768
Size: 9883
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2780 3990 4815 5077 928
Size: 971 881 1210 825 262 5734
Fragments arranged by size:
5734 1210 971 881 825 262
BbvCI CC'TCA_GC
Cuts at: 2031 2745 3821 2031
Size: 714 1076 8093
Fragments arranged by size:
8093 1076 714
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3542 3576 8074 8108 1903
Size: 34 1605 34 4498 34 3678
Fragments arranged by size:
4498 3678 1605 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2609 6422 7775 8985 2609
Size: 3813 1353 1210 3507
Fragments arranged by size:
3813 3507 1353 1210
BclI T'GATC_A
Cuts at: 1548 2143 1548
Size: 595 9288
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2492 2501 6755
Size: 107 122 71 631 605 820 9 4254
Cuts at: 6755 6771 8494 136
Size: 16 1723 1525
Fragments arranged by size:
4254 1723 1525 820 631 605 122 107
71 16 9
BglII A'GATC_T
Cuts at: 3285 5448 6501 6643 9878 3285
Size: 2163 1053 142 3235 3290
Fragments arranged by size:
3290 3235 2163 1053 142
BlpI GC'TnA_GC
Cuts at: 5393 7300 5393
Size: 1907 7976
Bme1580I G_kGCm'C
Cuts at: 1790 3063 5600 5669 7944 9100 1790
Size: 1273 2537 69 2275 1156 2573
Fragments arranged by size:
2573 2537 2275 1273 1156 69
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9883
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2820 2909 6434
Size: 416 53 83 399 24 1403 89 3525
Cuts at: 6434 6745 7033 7614 8572 442
Size: 311 288 581 958 1753
Fragments arranged by size:
3525 1753 1403 958 581 416 399 311
288 89 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3459 1056
Size: 2403 7480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2493 2732 2984 3729 4710 5357 6459
Size: 1073 181 239 252 745 981 647 1102
Cuts at: 6459 6745 6902 8543 1239
Size: 286 157 1641 2579
Fragments arranged by size:
2579 1641 1102 1073 981 745 647 286
252 239 181 157
Bpu10I CC'TnA_GC
Cuts at: 2031 2745 3543 3821 2031
Size: 714 798 278 8093
Fragments arranged by size:
8093 798 714 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3341 3533 3950 4175 4479 4808 5093 8008 8873
Size: 192 417 225 304 329 285 2915 865
Cuts at: 8873 9171 3341
Size: 298 4053
Fragments arranged by size:
4053 2915 865 417 329 304 298 285
225 192
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5474 6041 8546 882
Size: 617 3975 567 2505 2219
Fragments arranged by size:
3975 2505 2219 617 567
BsaAI yAC'GTr
Cuts at: 493 1204 6811 7674 493
Size: 711 5607 863 2702
Fragments arranged by size:
5607 2702 863 711
BsaBI GATnn'nnATC
Cuts at: 5253 9877 5253
Size: 4624 5259
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3765 4572 5536 7690
Size: 53 83 186 936 2232 807 964 2154
Cuts at: 7690 8072 8707 275
Size: 382 635 1451
Fragments arranged by size:
2232 2154 1451 964 936 807 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5415 6486 8316 8714 8988 9135 9546 5415
Size: 1071 1830 398 274 147 411 5752
Fragments arranged by size:
5752 1830 1071 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2705 2735 3149 3179 5108
Size: 30 165 30 1302 30 414 30 1929
Cuts at: 5108 5138 6457 6487 1178
Size: 30 1319 30 4574
Fragments arranged by size:
4574 1929 1319 1302 414 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2117 2132 2135 2138 2141 2144 2222
Size: 862 69 15 3 3 3 3 78
Cuts at: 2222 2333 2414 2496 2528 2531 2549 3542 3986
Size: 111 81 82 32 3 18 993 444
Cuts at: 3986 4079 4354 4874 5459 5957 6173 6350 1186
Size: 93 275 520 585 498 216 177 4719
Fragments arranged by size:
4719 993 862 585 520 498 444 275
216 177 111 93 82 81 78 69
32 18 15 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3062 3216 3593 5049 6622 6745 6828 6951 6957
Size: 154 377 1456 1573 123 83 123 6
Cuts at: 6957 7109 9086 9582 3062
Size: 152 1977 496 3363
Fragments arranged by size:
3363 1977 1573 1456 496 377 154 152
123 123 83 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4130 4193 4816 5853 1163
Size: 573 2394 63 623 1037 5193
Fragments arranged by size:
5193 2394 1037 623 573 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7333 8094 9122 1540
Size: 275 186 5332 761 1028 2301
Fragments arranged by size:
5332 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3549 7944 8029 9100 3549
Size: 4395 85 1071 4332
Fragments arranged by size:
4395 4332 1071 85
BsmI GAATG_Cn'
Cuts at: 2977 4338 6667 7394 7487 2977
Size: 1361 2329 727 93 5373
Fragments arranged by size:
5373 2329 1361 727 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4750 4750
Size: 9883
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2826 3063 3331 3549 3702 3804 3996
Size: 399 1036 237 268 218 153 102 192
Cuts at: 3996 5269 5600 5669 7202 7944 8029 9100 1391
Size: 1273 331 69 1533 742 85 1071 2174
Fragments arranged by size:
2174 1533 1273 1071 1036 742 399 331
268 237 218 192 153 102 85 69
BspEI T'CCGG_A
Cuts at: 5415 9546 5415
Size: 4131 5752
BspHI T'CATG_A
Cuts at: 5088 5805 7772 9502 5088
Size: 717 1967 1730 5469
Fragments arranged by size:
5469 1967 1730 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2892 3660 3805 4282 5852 6610 7238 844
Size: 2048 768 145 477 1570 758 628 3489
Fragments arranged by size:
3489 2048 1570 768 758 628 477 145
BsrBI CCG'CTC
Cuts at: 1394 1823 2907 4480 5430 7770 1394
Size: 429 1084 1573 950 2340 3507
Fragments arranged by size:
3507 2340 1573 1084 950 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4353 4932 5518 5523 5578 6702 7140
Size: 1183 3105 579 586 5 55 1124 438
Cuts at: 7140 8378 8552 65
Size: 1238 174 1396
Fragments arranged by size:
3105 1396 1238 1183 1124 586 579 438
174 55 5
BsrFI r'CCGG_y
Cuts at: 1700 1810 2358 4122 5714 8527 8714 1700
Size: 110 548 1764 1592 2813 187 2869
Fragments arranged by size:
2869 2813 1764 1592 548 187 110
BsrGI T'GTAC_A
Cuts at: 96 3573 4606 96
Size: 3477 1033 5373
Fragments arranged by size:
5373 3477 1033
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9883
BssSI C'ACGA_G
Cuts at: 3674 7943 8955 3674
Size: 4269 1012 4602
Fragments arranged by size:
4602 4269 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5219 6269 7048 794
Size: 4425 1050 779 3629
Fragments arranged by size:
4425 3629 1050 779
BstBI TT'CG_AA
Cuts at: 6219 8687 6219
Size: 2468 7415
BstEII G'GTnAC_C
Cuts at: 1184 5056 1184
Size: 3872 6011
BstXI CCAn_nnnn'nTGG
Cuts at: 3346 3593 6753 3346
Size: 247 3160 6476
Fragments arranged by size:
6476 3160 247
BstYI r'GATC_y
Cuts at: 1972 2961 3225 3285 5010 5448 6501 6553 6643
Size: 989 264 60 1725 438 1053 52 90
Cuts at: 6643 7213 7580 7965 7982 9434 9520 9532 9878
Size: 570 367 385 17 1452 86 12 346
Cuts at: 9878 1972
Size: 1977
Fragments arranged by size:
1977 1725 1452 1053 989 570 438 385
367 346 264 90 86 60 52 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2487 2499 3693 4134 6051 6258 513
Size: 552 1422 12 1194 441 1917 207 4138
Fragments arranged by size:
4138 1917 1422 1194 552 441 207 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 7164 487
Size: 683 56 5938 3206
Fragments arranged by size:
5938 3206 683 56
BtsI GCAGTG_nn'
Cuts at: 2227 3203 5220 5600 5794 6790 6805 7049 7399
Size: 976 2017 380 194 996 15 244 350
Cuts at: 7399 8193 8213 2227
Size: 794 20 3897
Fragments arranged by size:
3897 2017 996 976 794 380 350 244
194 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9883
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9848
DraI TTT'AAA
Cuts at: 3924 7187 3924
Size: 3263 6620
DraIII CAC_nnn'GTG
Cuts at: 4108 4384 5064 6198 4108
Size: 276 680 1134 7793
Fragments arranged by size:
7793 1134 680 276
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5708 8890 809
Size: 507 4392 3182 1802
Fragments arranged by size:
4392 3182 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2251 3418 3609
Size: 54 1425 50 275 18 421 1167 191
Cuts at: 3609 3711 4137 4179 4671 5759 6840 7330 8219
Size: 102 426 42 492 1088 1081 490 889
Cuts at: 8219 9848 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1167 1088 1081 889 492 490
426 421 275 191 102 54 50 43
42 18
EagI C'GGCC_G
Cuts at: 1537 1812 7330 1537
Size: 275 5518 4090
Fragments arranged by size:
5518 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2255 2712 3480 4470 5325 6970 7813
Size: 741 161 457 768 990 855 1645 843
Cuts at: 7813 1353
Size: 3423
Fragments arranged by size:
3423 1645 990 855 843 768 741 457
161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2614 7052 7087 8470 8844 8990
Size: 1064 138 1025 4438 35 1383 374 146
Cuts at: 8990 387
Size: 1280
Fragments arranged by size:
4438 1383 1280 1064 1025 374 146 138
35
EcoICRI GAG'CTC
Cuts at: 3547 3547
Size: 9883
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2493 2732 2949 2984 3031 3491
Size: 393 680 181 239 217 35 47 460
Cuts at: 3491 3729 4002 4230 4710 5027 5036 5357 5940
Size: 238 273 228 480 317 9 321 583
Cuts at: 5940 6163 6459 6745 6902 7946 8543 9330 9598
Size: 223 296 286 157 1044 597 787 268
Cuts at: 9598 1239
Size: 1524
Fragments arranged by size:
1524 1044 787 680 597 583 480 460
393 321 317 296 286 273 268 239
238 228 223 217 181 157 47 35
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2315 4989 6561 2315
Size: 2674 1572 5637
Fragments arranged by size:
5637 2674 1572
EcoO109I rG'GnC_Cy
Cuts at: 2494 3059 3088 3539 4768 4982 4995 5075 5410
Size: 565 29 451 1229 214 13 80 335
Cuts at: 5410 6277 6591 2494
Size: 867 314 5786
Fragments arranged by size:
5786 1229 867 565 451 335 314 214
80 29 13
EcoRI G'AATT_C
Cuts at: 6215 7192 7570 6215
Size: 977 378 8528
Fragments arranged by size:
8528 977 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6388 6388
Size: 9883
FspI TGC'GCA
Cuts at: 8389 8389
Size: 9883
HaeII r_GCGC'y
Cuts at: 1678 3768 9030 1678
Size: 2090 5262 2531
Fragments arranged by size:
5262 2531 2090
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2554 3353 3385 4728
Size: 32 135 32 950 32 799 32 1343
Cuts at: 4728 4760 5108 5140 6226 6258 8524 8556 8598
Size: 32 348 32 1086 32 2266 32 42
Cuts at: 8598 8630 1373
Size: 32 2626
Fragments arranged by size:
2626 2266 1343 1086 950 799 348 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4660 4924 5463 7227 7473 669
Size: 988 281 2722 264 539 1764 246 3079
Fragments arranged by size:
3079 2722 1764 988 539 281 264 246
HindIII A'AGCT_T
Cuts at: 748 2606 748
Size: 1858 8025
HpaI GTT'AAC
Cuts at: 5463 7473 5463
Size: 2010 7873
KasI G'GCGC_C
Cuts at: 3764 3764
Size: 9883
KpnI G_GTAC'C
Cuts at: 2075 6493 7208 2075
Size: 4418 715 4750
Fragments arranged by size:
4750 4418 715
MfeI C'AATT_G
Cuts at: 4255 7482 4255
Size: 3227 6656
MluI A'CGCG_T
Cuts at: 9759 9759
Size: 9883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3032 6100 7026 8997 9181 1305
Size: 1727 3068 926 1971 184 2007
Fragments arranged by size:
3068 2007 1971 1727 926 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2253 3611 3713 4139 4181
Size: 54 1425 343 421 1358 102 426 42
Cuts at: 4181 4673 6842 10
Size: 492 2169 3051
Fragments arranged by size:
3051 2169 1425 1358 492 426 421 343
102 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 3174 3344 6147 7133 7841 8200 8359
Size: 648 2008 170 2803 986 708 359 159
Cuts at: 8359 518
Size: 2042
Fragments arranged by size:
2803 2042 2008 986 708 648 359 170
159
MspA1I CmG'CkG
Cuts at: 1606 3220 3670 3695 4633 5053 5658 5968 6312
Size: 1614 450 25 938 420 605 310 344
Cuts at: 6312 7087 7299 7976 9124 9369 1606
Size: 775 212 677 1148 245 2120
Fragments arranged by size:
2120 1614 1148 938 775 677 605 450
420 344 310 245 212 25
NarI GG'CG_CC
Cuts at: 3765 3765
Size: 9883
NcoI C'CATG_G
Cuts at: 513 1065 2487 2499 4134 6051 6258 513
Size: 552 1422 12 1635 1917 207 4138
Fragments arranged by size:
4138 1917 1635 1422 552 207 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9883
NheI G'CTAG_C
Cuts at: 1052 3455 1052
Size: 2403 7480
NotI GC'GGCC_GC
Cuts at: 7330 7330
Size: 9883
NspI r_CATG'y
Cuts at: 1132 1229 3574 4850 6146 7241 8786 1132
Size: 97 2345 1276 1296 1095 1545 2229
Fragments arranged by size:
2345 2229 1545 1296 1276 1095 97
PasI CC'CwG_GG
Cuts at: 1348 3091 5128 5542 1348
Size: 1743 2037 414 5689
Fragments arranged by size:
5689 2037 1743 414
PciI A'CATG_T
Cuts at: 3570 8782 3570
Size: 5212 4671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3373 6145 6198 6259 7023 1108
Size: 2265 2772 53 61 764 3968
Fragments arranged by size:
3968 2772 2265 764 61 53
PfoI T'CCnGG_A
Cuts at: 2454 2611 3000 4713 4764 7164 2454
Size: 157 389 1713 51 2400 5173
Fragments arranged by size:
5173 2400 1713 389 157 51
PmeI GTTT'AAAC
Cuts at: 7187 7187
Size: 9883
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4694 4726 7952 7984 9465 9497 4694
Size: 32 3226 32 1481 32 5080
Fragments arranged by size:
5080 3226 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4768 4982 4995 5075 5410 6277 6591 4768
Size: 214 13 80 335 867 314 8060
Fragments arranged by size:
8060 867 335 314 214 80 13
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9883
PsiI TTA'TAA
Cuts at: 7453 7453
Size: 9883
PspOMI G'GGCC_C
Cuts at: 3059 3059
Size: 9883
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4137 4169 4137
Size: 32 9851
PstI C_TGCA'G
Cuts at: 830 3789 5263 5929 6311 7053 7235 830
Size: 2959 1474 666 382 742 182 3478
Fragments arranged by size:
3478 2959 1474 742 666 382 182
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9883
PvuII CAG'CTG
Cuts at: 3220 4633 5053 3220
Size: 1413 420 8050
Fragments arranged by size:
8050 1413 420
SacI G_AGCT'C
Cuts at: 3549 3549
Size: 9883
SacII CC_GC'GG
Cuts at: 3696 3696
Size: 9883
SalI G'TCGA_C
Cuts at: 1936 7225 1936
Size: 5289 4594
SanDI GG'GwC_CC
Cuts at: 4995 5075 4995
Size: 80 9803
SapI GCTCTTCn'nnn_
Cuts at: 4470 5325 4470
Size: 855 9028
SbfI CC_TGCA'GG
Cuts at: 5263 7235 5263
Size: 1972 7911
ScaI AGT'ACT
Cuts at: 8131 9843 8131
Size: 1712 8171
SexAI A'CCwGG_T
Cuts at: 4104 4104
Size: 9883
SfcI C'TryA_G
Cuts at: 826 1045 2965 3785 4237 5259 5925 6307 7049
Size: 219 1920 820 452 1022 666 382 742
Cuts at: 7049 7231 8366 9047 9238 826
Size: 182 1135 681 191 1471
Fragments arranged by size:
1920 1471 1135 1022 820 742 681 666
452 382 219 191 182
SfiI GGCCn_nnn'nGGCC
Cuts at: 2492 2492
Size: 9883
SfoI GGC'GCC
Cuts at: 3766 3766
Size: 9883
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9883
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9883
SmaI CCC'GGG
Cuts at: 1872 6275 6440 7210 1872
Size: 4403 165 770 4545
Fragments arranged by size:
4545 4403 770 165
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3356 3548 3965 4190 4494 4823
Size: 197 926 1413 192 417 225 304 329
Cuts at: 4823 5036 5108 7987 8888 9150 820
Size: 213 72 2879 901 262 1553
Fragments arranged by size:
2879 1553 1413 926 901 417 329 304
262 225 213 197 192 72
SnaBI TAC'GTA
Cuts at: 493 7674 493
Size: 7181 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9883
SphI G_CATG'C
Cuts at: 1132 7241 1132
Size: 6109 3774
SspI AAT'ATT
Cuts at: 5 52 7807 5
Size: 47 7755 2081
Fragments arranged by size:
7755 2081 47
StuI AGG'CCT
Cuts at: 1517 4235 4955 1517
Size: 2718 720 6445
Fragments arranged by size:
6445 2718 720
StyI C'CwwG_G
Cuts at: 513 1065 2487 2499 3702 3714 4134 4215 4327
Size: 552 1422 12 1203 12 420 81 112
Cuts at: 4327 6051 6258 6721 7322 513
Size: 1724 207 463 601 3074
Fragments arranged by size:
3074 1724 1422 1203 601 552 463 420
207 112 81 12 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2318 3039 6015 7910 7927 8080 8265
Size: 1255 738 721 2976 1895 17 153 185
Cuts at: 8265 8710 325
Size: 445 1498
Fragments arranged by size:
2976 1895 1498 1255 738 721 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3573 4606 4878
Size: 274 80 33 51 1443 1596 1033 272
Cuts at: 4878 8129 9841 96
Size: 3251 1712 138
Fragments arranged by size:
3251 1712 1596 1443 1033 274 272 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3194 3226 6109 6141 6193 6225 9843 9875 3194
Size: 32 2883 32 52 32 3618 32 3202
Fragments arranged by size:
3618 3202 2883 52 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9883
XbaI T'CTAG_A
Cuts at: 7219 7219
Size: 9883
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2092 4970 5068 6773 7162 2092
Size: 2878 98 1705 389 4813
Fragments arranged by size:
4813 2878 1705 389 98
XhoI C'TCGA_G
Cuts at: 1943 5036 1943
Size: 3093 6790
XmaI C'CCGG_G
Cuts at: 1870 6273 6438 7208 1870
Size: 4403 165 770 4545
Fragments arranged by size:
4545 4403 770 165
XmnI GAAnn'nnTTC
Cuts at: 8012 8012
Size: 9883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7691 276
Size: 53 83 186 936 6157 2468
Fragments arranged by size:
6157 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI RsrII SrfI SwaI