Restriction map for
pFN21AA0160
(Circular) (Six-base) MAPSORT of: pFN21AA0160.seq Check: 1 from: 1 to: 6922
pFN21AA0160 6922 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2629 2629
Size: 6922
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4732 278
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4265 1937
Size: 2328 4594
Acc65I G'GTAC_C
Cuts at: 4243 4243
Size: 6922
AclI AA'CG_TT
Cuts at: 1563 5049 5422 1563
Size: 3486 373 3063
Fragments arranged by size:
3486 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3351 3663 4131 4985 6369 6637 1632
Size: 1719 312 468 854 1384 268 1917
Fragments arranged by size:
1917 1719 1384 854 468 312 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6725
AflIII A'CryG_T
Cuts at: 1203 2355 5821 6798 1203
Size: 1152 3466 977 1327
Fragments arranged by size:
3466 1327 1152 977
AgeI A'CCGG_T
Cuts at: 2212 5753 2212
Size: 3541 3381
AhdI GACnn_n'nnGTC
Cuts at: 5651 5651
Size: 6922
AleI CACnn'nnGTG
Cuts at: 1166 2025 1166
Size: 859 6063
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6922
AlwNI CAG_nnn'CTG
Cuts at: 4665 6237 4665
Size: 1572 5350
ApaI G_GGCC'C
Cuts at: 2052 2052
Size: 6922
ApaLI G'TGCA_C
Cuts at: 4979 6135 4979
Size: 1156 5766
ApoI r'AATT_y
Cuts at: 1493 2542 2680 2711 3145 3629 3955 4231 4457
Size: 1049 138 31 434 484 326 276 226
Cuts at: 4457 4609 6718 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1049 484 434 326 276 226
152 138 31
AseI AT'TA_AT
Cuts at: 160 1032 2802 5476 160
Size: 872 1770 2674 1606
Fragments arranged by size:
2674 1770 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
AvaI C'yCGr_G
Cuts at: 1870 1943 2044 4247 1870
Size: 73 101 2203 4545
Fragments arranged by size:
4545 2203 101 73
BamHI G'GATC_C
Cuts at: 3766 4252 4619 3766
Size: 486 367 6069
Fragments arranged by size:
6069 486 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2641 4243 5698 618
Size: 325 217 627 221 633 1602 1455 1842
Fragments arranged by size:
1842 1602 1455 633 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2052 4241 1391
Size: 661 2189 4072
Fragments arranged by size:
4072 2189 661
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 6922
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5951
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5113 5147 1903
Size: 34 3176 34 3678
Fragments arranged by size:
3678 3176 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3247 4814 6024 3247
Size: 1567 1210 4145
Fragments arranged by size:
4145 1567 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6922
BfrBI ATG'CAT
Cuts at: 3994 3994
Size: 6922
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5533 136
Size: 107 122 71 631 605 3861 1525
Fragments arranged by size:
3861 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2289 3229 6917 2289
Size: 940 3688 2294
Fragments arranged by size:
3688 2294 940
BlpI GC'TnA_GC
Cuts at: 4339 4339
Size: 6922
Bme1580I G_kGCm'C
Cuts at: 1790 2052 4983 6139 1790
Size: 262 2931 1156 2573
Fragments arranged by size:
2931 2573 1156 262
BmgBI CAC'GTC
Cuts at: 1318 2239 1318
Size: 921 6001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4653 5611 442
Size: 416 53 83 399 24 3236 958 1753
Fragments arranged by size:
3236 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6922
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3051 3051
Size: 6922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2356 5582 1239
Size: 1010 107 3226 2579
Fragments arranged by size:
3226 2579 1010 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3380 5047 5912 6210 3380
Size: 1667 865 298 4092
Fragments arranged by size:
4092 1667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3089 5585 882
Size: 617 1590 2496 2219
Fragments arranged by size:
2496 2219 1590 617
BsaAI yAC'GTr
Cuts at: 493 1204 4713 493
Size: 711 3509 2702
Fragments arranged by size:
3509 2702 711
BsaBI GATnn'nnATC
Cuts at: 3522 6916 3522
Size: 3394 3528
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4729 5111 5746
Size: 53 83 186 936 476 2720 382 635
Cuts at: 5746 275
Size: 1451
Fragments arranged by size:
2720 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2212 5355 5753 6027 6174 6585 2212
Size: 3143 398 274 147 411 2549
Fragments arranged by size:
3143 2549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2150 2180 1178
Size: 30 165 30 747 30 5920
Fragments arranged by size:
5920 747 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2158 2161 2164 2167 1186
Size: 972 3 3 3 5941
Fragments arranged by size:
5941 972 3 3 3
BseYI C'CCAG_C
Cuts at: 2051 6125 6621 2051
Size: 4074 496 2352
Fragments arranged by size:
4074 2352 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3616 1163
Size: 573 1880 4469
Fragments arranged by size:
4469 1880 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4372 5133 6161 1540
Size: 275 186 2371 761 1028 2301
Fragments arranged by size:
2371 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2238 4983 5068 6139 2238
Size: 2745 85 1071 3021
Fragments arranged by size:
3021 2745 1071 85
BsmI GAATG_Cn'
Cuts at: 4433 4526 4433
Size: 93 6829
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2238 4241 4983 5068 6139 1391
Size: 399 262 186 2003 742 85 1071 2174
Fragments arranged by size:
2174 2003 1071 742 399 262 186 85
BspEI T'CCGG_A
Cuts at: 6585 6585
Size: 6922
BspHI T'CATG_A
Cuts at: 3867 4811 6541 3867
Size: 944 1730 4248
Fragments arranged by size:
4248 1730 944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2629 3354 3722 4277 844
Size: 1785 725 368 555 3489
Fragments arranged by size:
3489 1785 725 555 368
BsrBI CCG'CTC
Cuts at: 1394 1823 4809 1394
Size: 429 2986 3507
Fragments arranged by size:
3507 2986 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3349 5417 5591 65
Size: 1183 2101 2068 174 1396
Fragments arranged by size:
2101 2068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2212 5566 5753 1700
Size: 110 402 3354 187 2869
Fragments arranged by size:
3354 2869 402 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6922
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6922
BssSI C'ACGA_G
Cuts at: 2252 4982 5994 2252
Size: 2730 1012 3180
Fragments arranged by size:
3180 2730 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6922
BstBI TT'CG_AA
Cuts at: 2305 3042 5726 2305
Size: 737 2684 3501
Fragments arranged by size:
3501 2684 737
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6922
BstYI r'GATC_y
Cuts at: 1972 2289 3229 3766 4252 4619 5004 5021 6473
Size: 317 940 537 486 367 385 17 1452
Cuts at: 6473 6559 6571 6917 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 940 537 486 385 367 346
317 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3513 3513
Size: 6922
BtgI C'CryG_G
Cuts at: 513 1065 2004 2181 513
Size: 552 939 177 5254
Fragments arranged by size:
5254 939 552 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3509 487
Size: 683 56 2283 3900
Fragments arranged by size:
3900 2283 683 56
BtsI GCAGTG_nn'
Cuts at: 4438 5232 5252 4438
Size: 794 20 6108
Fragments arranged by size:
6108 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6922
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6887
DraI TTT'AAA
Cuts at: 2401 4226 2401
Size: 1825 5097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5929 809
Size: 507 4613 1802
Fragments arranged by size:
4613 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3682 4369 5258
Size: 54 1425 50 275 18 1852 687 889
Cuts at: 5258 6887 8
Size: 1629 43
Fragments arranged by size:
1852 1629 1425 889 687 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4369 1537
Size: 275 2557 4090
Fragments arranged by size:
4090 2557 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2776 2832 3020 3084 3801 4852 1353
Size: 1423 56 188 64 717 1051 3423
Fragments arranged by size:
3423 1423 1051 717 188 64 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5509 5883 6029 387
Size: 1064 138 3920 374 146 1280
Fragments arranged by size:
3920 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2356 3351 3663 4131 4985 5582
Size: 393 617 107 995 312 468 854 597
Cuts at: 5582 6369 6637 1239
Size: 787 268 1524
Fragments arranged by size:
1524 995 854 787 617 597 468 393
312 268 107
EcoO109I rG'GnC_Cy
Cuts at: 2048 3059 2048
Size: 1011 5911
EcoRI G'AATT_C
Cuts at: 4231 4609 4231
Size: 378 6544
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2582 2582
Size: 6922
FspI TGC'GCA
Cuts at: 5428 5428
Size: 6922
HaeII r_GCGC'y
Cuts at: 1678 2012 2926 6069 1678
Size: 334 914 3143 2531
Fragments arranged by size:
3143 2531 914 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2402 2434 2435 2467 2501
Size: 32 135 32 830 32 1 32 34
Cuts at: 2501 2533 3145 3177 3214 3246 4169 4201 5563
Size: 32 612 32 37 32 923 32 1362
Cuts at: 5563 5595 5637 5669 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1362 923 830 612 135 42 37
34 32 32 32 32 32 32 32
32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4266 4512 669
Size: 988 281 2328 246 3079
Fragments arranged by size:
3079 2328 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4164 748
Size: 3416 3506
HpaI GTT'AAC
Cuts at: 4512 4512
Size: 6922
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 6922
KpnI G_GTAC'C
Cuts at: 4247 4247
Size: 6922
MfeI C'AATT_G
Cuts at: 4521 4521
Size: 6922
MluI A'CGCG_T
Cuts at: 6798 6798
Size: 6922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3065 6036 6220 1305
Size: 1760 2971 184 2007
Fragments arranged by size:
2971 2007 1760 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3684 10
Size: 54 1425 343 1852 3248
Fragments arranged by size:
3248 1852 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2025 2505 4880 5239 5398 518
Size: 648 859 480 2375 359 159 2042
Fragments arranged by size:
2375 2042 859 648 480 359 159
MspA1I CmG'CkG
Cuts at: 1606 2183 2192 4338 5015 6163 6408 1606
Size: 577 9 2146 677 1148 245 2120
Fragments arranged by size:
2146 2120 1148 677 577 245 9
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 6922
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5431
Fragments arranged by size:
5431 939 552
NdeI CA'TA_TG
Cuts at: 387 2733 387
Size: 2346 4576
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6922
NotI GC'GGCC_GC
Cuts at: 4369 4369
Size: 6922
NsiI A_TGCA'T
Cuts at: 3996 3996
Size: 6922
NspI r_CATG'y
Cuts at: 1132 1229 2359 3625 3994 4280 5825 1132
Size: 97 1130 1266 369 286 1545 2229
Fragments arranged by size:
2229 1545 1266 1130 369 286 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6922
PciI A'CATG_T
Cuts at: 2355 5821 2355
Size: 3466 3456
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6922
PfoI T'CCnGG_A
Cuts at: 2773 2773
Size: 6922
PmeI GTTT'AAAC
Cuts at: 4226 4226
Size: 6922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4991 5023 6504 6536 4991
Size: 32 1481 32 5377
Fragments arranged by size:
5377 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3059 3059
Size: 6922
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6922
PsiI TTA'TAA
Cuts at: 4492 4492
Size: 6922
PspOMI G'GGCC_C
Cuts at: 2048 2048
Size: 6922
PstI C_TGCA'G
Cuts at: 830 4274 830
Size: 3444 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6922
SacII CC_GC'GG
Cuts at: 2184 2184
Size: 6922
SalI G'TCGA_C
Cuts at: 1936 4264 1936
Size: 2328 4594
SapI GCTCTTCn'nnn_
Cuts at: 2832 2832
Size: 6922
SbfI CC_TGCA'GG
Cuts at: 4274 4274
Size: 6922
ScaI AGT'ACT
Cuts at: 5170 6882 5170
Size: 1712 5210
SfcI C'TryA_G
Cuts at: 826 1045 2293 3225 4270 5405 6086 6277 826
Size: 219 1248 932 1045 1135 681 191 1471
Fragments arranged by size:
1471 1248 1135 1045 932 681 219 191
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 6922
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6922
SmaI CCC'GGG
Cuts at: 1872 4249 1872
Size: 2377 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3395 5026 5927 6189 820
Size: 197 926 1452 1631 901 262 1553
Fragments arranged by size:
1631 1553 1452 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4713 493
Size: 4220 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6922
SphI G_CATG'C
Cuts at: 1132 3994 4280 1132
Size: 2862 286 3774
Fragments arranged by size:
3774 2862 286
SspI AAT'ATT
Cuts at: 5 52 2331 2824 3288 4846 5
Size: 47 2279 493 464 1558 2081
Fragments arranged by size:
2279 2081 1558 493 464 47
StuI AGG'CCT
Cuts at: 1517 2270 1517
Size: 753 6169
StyI C'CwwG_G
Cuts at: 513 1065 2004 3356 4361 513
Size: 552 939 1352 1005 3074
Fragments arranged by size:
3074 1352 1005 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4949 4966 5119 5304 5749 325
Size: 1255 3369 17 153 185 445 1498
Fragments arranged by size:
3369 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3362 5168 6880
Size: 274 80 33 51 1443 1385 1806 1712
Cuts at: 6880 96
Size: 138
Fragments arranged by size:
1806 1712 1443 1385 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6882 6914 6882
Size: 32 6890
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6922
XbaI T'CTAG_A
Cuts at: 4258 4258
Size: 6922
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2965 2965
Size: 6922
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6922
XmaI C'CCGG_G
Cuts at: 1870 4247 1870
Size: 2377 4545
XmnI GAAnn'nnTTC
Cuts at: 2841 5051 2841
Size: 2210 4712
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4730 276
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI
PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI