Restriction map for
pFN21AA0151
(Circular) (Six-base) MAPSORT of: pFN21AA0151.seq Check: 4668 from: 1 to: 6853
pFN21AA0151 6853 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2149 2388 2735 2810 3763 3873 2149
Size: 239 347 75 953 110 5129
Fragments arranged by size:
5129 953 347 239 110 75
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3201 4663 278
Size: 53 83 186 936 1665 1462 2468
Fragments arranged by size:
2468 1665 1462 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4196 1937
Size: 2259 4594
Acc65I G'GTAC_C
Cuts at: 4174 4174
Size: 6853
AclI AA'CG_TT
Cuts at: 1563 4980 5353 1563
Size: 3417 373 3063
Fragments arranged by size:
3417 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3133 3163 3491 3950 4916 6300 6568 1632
Size: 1501 30 328 459 966 1384 268 1917
Fragments arranged by size:
1917 1501 1384 966 459 328 268 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6656
AflIII A'CryG_T
Cuts at: 1203 2553 5752 6729 1203
Size: 1350 3199 977 1327
Fragments arranged by size:
3199 1350 1327 977
AgeI A'CCGG_T
Cuts at: 5684 5684
Size: 6853
AhdI GACnn_n'nnGTC
Cuts at: 5582 5582
Size: 6853
AleI CACnn'nnGTG
Cuts at: 1166 3078 1166
Size: 1912 4941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6853
AlwNI CAG_nnn'CTG
Cuts at: 2651 3280 3469 4596 6168 2651
Size: 629 189 1127 1572 3336
Fragments arranged by size:
3336 1572 1127 629 189
ApaI G_GGCC'C
Cuts at: 2772 3298 2772
Size: 526 6327
ApaLI G'TGCA_C
Cuts at: 3020 3845 4910 6066 3020
Size: 825 1065 1156 3807
Fragments arranged by size:
3807 1156 1065 825
ApoI r'AATT_y
Cuts at: 1493 3742 4162 4388 4540 6649 1493
Size: 2249 420 226 152 2109 1697
Fragments arranged by size:
2249 2109 1697 420 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5407 160
Size: 872 4375 1606
Fragments arranged by size:
4375 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
AvaI C'yCGr_G
Cuts at: 1870 1943 2487 3086 4178 1870
Size: 73 544 599 1092 4545
Fragments arranged by size:
4545 1092 599 544 73
BamHI G'GATC_C
Cuts at: 4183 4550 4183
Size: 367 6486
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2747 2836 3263 4174 5629
Size: 325 217 627 960 89 427 911 1455
Cuts at: 5629 618
Size: 1842
Fragments arranged by size:
1842 1455 960 911 627 427 325 217
89
BanII G_rGCy'C
Cuts at: 1391 2306 2772 3093 3298 3988 4062 4172 1391
Size: 915 466 321 205 690 74 110 4072
Fragments arranged by size:
4072 915 690 466 321 205 110 74
BbeI G_GCGC'C
Cuts at: 3267 3267
Size: 6853
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2113 2935 928
Size: 971 214 822 4846
Fragments arranged by size:
4846 971 822 214
BbvCI CC'TCA_GC
Cuts at: 3407 3407
Size: 6853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5044 5078 1903
Size: 34 50 34 3023 34 3678
Fragments arranged by size:
3678 3023 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4745 5955 4745
Size: 1210 5643
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6853
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3876 5464 136
Size: 107 122 71 631 605 2204 1588 1525
Fragments arranged by size:
2204 1588 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6848 6848
Size: 6853
BlpI GC'TnA_GC
Cuts at: 4270 4270
Size: 6853
Bme1580I G_kGCm'C
Cuts at: 1790 2752 2772 2841 3024 3298 3849 4914 6070
Size: 962 20 69 183 274 551 1065 1156
Cuts at: 6070 1790
Size: 2573
Fragments arranged by size:
2573 1156 1065 962 551 274 183 69
20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6853
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2825 3346 4584
Size: 416 53 83 399 24 1408 521 1238
Cuts at: 4584 5542 442
Size: 958 1753
Fragments arranged by size:
1753 1408 1238 958 521 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6853
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 3538 2287
Size: 1251 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2257 2318 2638 2795 2879 3338 3508 5513
Size: 1018 61 320 157 84 459 170 2005
Cuts at: 5513 1239
Size: 2579
Fragments arranged by size:
2579 2005 1018 459 320 170 157 84
61
Bpu10I CC'TnA_GC
Cuts at: 3407 3563 3987 3407
Size: 156 424 6273
Fragments arranged by size:
6273 424 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4978 5843 6141 4978
Size: 865 298 5690
Fragments arranged by size:
5690 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2900 3542 3845 5516 882
Size: 617 1401 642 303 1671 2219
Fragments arranged by size:
2219 1671 1401 642 617 303
BsaAI yAC'GTr
Cuts at: 493 1204 4644 493
Size: 711 3440 2702
Fragments arranged by size:
3440 2702 711
BsaBI GATnn'nnATC
Cuts at: 6847 6847
Size: 6853
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3198 3264 4660 5042
Size: 53 83 186 936 1665 66 1396 382
Cuts at: 5042 5677 275
Size: 635 1451
Fragments arranged by size:
1665 1451 1396 936 635 382 186 83
66 53
BsaWI w'CCGG_w
Cuts at: 2096 2709 5286 5684 5958 6105 6516 2096
Size: 613 2577 398 274 147 411 2433
Fragments arranged by size:
2577 2433 613 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2091 2121 2690 2720 3305
Size: 30 165 30 688 30 569 30 585
Cuts at: 3305 3335 1178
Size: 30 4696
Fragments arranged by size:
4696 688 585 569 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 2459 2549 3389 3728 3800 1186
Size: 1054 219 90 840 339 72 4239
Fragments arranged by size:
4239 1054 840 339 219 90 72
BseYI C'CCAG_C
Cuts at: 2047 2583 2732 2880 3092 4019 4135 6056 6552
Size: 536 149 148 212 927 116 1921 496
Cuts at: 6552 2047
Size: 2348
Fragments arranged by size:
2348 1921 927 536 496 212 149 148
116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2177 2951 3317 3815 1163
Size: 573 441 774 366 498 4201
Fragments arranged by size:
4201 774 573 498 441 366
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4303 5064 6092 1540
Size: 275 186 2302 761 1028 2301
Fragments arranged by size:
2302 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2015 3024 3354 3849 4062 4914 4999 6070 2015
Size: 1009 330 495 213 852 85 1071 2798
Fragments arranged by size:
2798 1071 1009 852 495 330 213 85
BsmI GAATG_Cn'
Cuts at: 2317 4364 4457 2317
Size: 2047 93 4713
Fragments arranged by size:
4713 2047 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2222 2521 3103 2222
Size: 299 582 5972
Fragments arranged by size:
5972 582 299
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2306 2752 2772 2841 3024 3093
Size: 399 225 291 446 20 69 183 69
Cuts at: 3093 3298 3354 3849 3988 4062 4172 4914 4999
Size: 205 56 495 139 74 110 742 85
Cuts at: 4999 6070 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 495 446 399 291 225
205 183 139 110 85 74 69 69
56 20
BspEI T'CCGG_A
Cuts at: 2096 6516 2096
Size: 4420 2433
BspHI T'CATG_A
Cuts at: 4742 6472 4742
Size: 1730 5123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2052 2148 2149 2388 2550 2735 2810 3234
Size: 1208 96 1 239 162 185 75 424
Cuts at: 3234 3763 3873 4047 4208 844
Size: 529 110 174 161 3489
Fragments arranged by size:
3489 1208 529 424 239 185 174 162
161 110 96 75 1
BsrBI CCG'CTC
Cuts at: 1394 1823 2563 4740 1394
Size: 429 740 2177 3507
Fragments arranged by size:
3507 2177 740 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2739 5348 5522 65
Size: 1183 1491 2609 174 1396
Fragments arranged by size:
2609 1491 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2241 2366 2728 3266 5497 5684 1700
Size: 110 431 125 362 538 2231 187 2869
Fragments arranged by size:
2869 2231 538 431 362 187 125 110
BsrGI T'GTAC_A
Cuts at: 96 2073 96
Size: 1977 4876
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6853
BssSI C'ACGA_G
Cuts at: 3848 4913 5925 3848
Size: 1065 1012 4776
Fragments arranged by size:
4776 1065 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6853
BstBI TT'CG_AA
Cuts at: 2575 5657 2575
Size: 3082 3771
BstEII G'GTnAC_C
Cuts at: 1184 3071 1184
Size: 1887 4966
BstXI CCAn_nnnn'nTGG
Cuts at: 2857 2857
Size: 6853
BstYI r'GATC_y
Cuts at: 1972 2611 3459 4183 4550 4935 4952 6404 6490
Size: 639 848 724 367 385 17 1452 86
Cuts at: 6490 6502 6848 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 848 724 639 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2328 3165 2328
Size: 837 6016
BtgI C'CryG_G
Cuts at: 513 1065 2684 2718 513
Size: 552 1619 34 4648
Fragments arranged by size:
4648 1619 552 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2387 3097 487
Size: 683 56 1161 710 4243
Fragments arranged by size:
4243 1161 710 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 3243 4369 5163 5183 2061
Size: 1182 1126 794 20 3731
Fragments arranged by size:
3731 1182 1126 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6853
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6818
DraI TTT'AAA
Cuts at: 4157 4157
Size: 6853
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5860 809
Size: 507 4544 1802
Fragments arranged by size:
4544 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2364 2847 4300
Size: 54 1425 50 275 18 534 483 1453
Cuts at: 4300 5189 6818 8
Size: 889 1629 43
Fragments arranged by size:
1629 1453 1425 889 534 483 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4300 1537
Size: 275 2488 4090
Fragments arranged by size:
4090 2488 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3152 3584 3939 4783 1353
Size: 1799 432 355 844 3423
Fragments arranged by size:
3423 1799 844 432 355
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2250 2704 3248 5440 5814 5960
Size: 1064 138 661 454 544 2192 374 146
Cuts at: 5960 387
Size: 1280
Fragments arranged by size:
2192 1280 1064 661 544 454 374 146
138
EcoICRI GAG'CTC
Cuts at: 4060 4060
Size: 6853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2257 2318 2638 2795 2879 3133 3163
Size: 393 625 61 320 157 84 254 30
Cuts at: 3163 3338 3491 3508 3950 4916 5513 6300 6568
Size: 175 153 17 442 966 597 787 268
Cuts at: 6568 1239
Size: 1524
Fragments arranged by size:
1524 966 787 625 597 442 393 320
268 254 175 157 153 84 61 30
17
EcoNI CCTnn'n_nnAGG
Cuts at: 2954 3065 2954
Size: 111 6742
EcoO109I rG'GnC_Cy
Cuts at: 2176 2769 3184 3277 3295 3517 4036 2176
Size: 593 415 93 18 222 519 4993
Fragments arranged by size:
4993 593 519 415 222 93 18
EcoRI G'AATT_C
Cuts at: 4162 4540 4162
Size: 378 6475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3673 3673
Size: 6853
FspI TGC'GCA
Cuts at: 5359 5359
Size: 6853
HaeII r_GCGC'y
Cuts at: 1678 2484 3267 6000 1678
Size: 806 783 2733 2531
Fragments arranged by size:
2733 2531 806 783
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2596 2628 2919 2951 3583
Size: 32 135 32 1024 32 291 32 632
Cuts at: 3583 3615 5494 5526 5568 5600 1373
Size: 32 1879 32 42 32 2626
Fragments arranged by size:
2626 1879 1024 632 291 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4197 4443 669
Size: 988 281 2259 246 3079
Fragments arranged by size:
3079 2259 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6853
HpaI GTT'AAC
Cuts at: 4443 4443
Size: 6853
KasI G'GCGC_C
Cuts at: 3263 3263
Size: 6853
KpnI G_GTAC'C
Cuts at: 4178 4178
Size: 6853
MfeI C'AATT_G
Cuts at: 4452 4452
Size: 6853
MluI A'CGCG_T
Cuts at: 6729 6729
Size: 6853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5967 6151 1305
Size: 4662 184 2007
Fragments arranged by size:
4662 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2849 10
Size: 54 1425 343 1017 4014
Fragments arranged by size:
4014 1425 1017 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2037 2627 3078 3347 4811 5170 5329
Size: 648 871 590 451 269 1464 359 159
Cuts at: 5329 518
Size: 2042
Fragments arranged by size:
2042 1464 871 648 590 451 359 269
159
MspA1I CmG'CkG
Cuts at: 1606 2809 2833 4269 4946 6094 6339 1606
Size: 1203 24 1436 677 1148 245 2120
Fragments arranged by size:
2120 1436 1203 1148 677 245 24
NaeI GCC'GGC
Cuts at: 2243 2368 2730 3268 2243
Size: 125 362 538 5828
Fragments arranged by size:
5828 538 362 125
NarI GG'CG_CC
Cuts at: 3264 3264
Size: 6853
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6301
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6853
NgoMIV G'CCGG_C
Cuts at: 2241 2366 2728 3266 2241
Size: 125 362 538 5828
Fragments arranged by size:
5828 538 362 125
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6853
NotI GC'GGCC_GC
Cuts at: 4300 4300
Size: 6853
NruI TCG'CGA
Cuts at: 2406 2406
Size: 6853
NspI r_CATG'y
Cuts at: 1132 1229 2557 3366 4054 4211 5756 1132
Size: 97 1328 809 688 157 1545 2229
Fragments arranged by size:
2229 1545 1328 809 688 157 97
PasI CC'CwG_GG
Cuts at: 1348 3910 1348
Size: 2562 4291
PciI A'CATG_T
Cuts at: 2553 5752 2553
Size: 3199 3654
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2880 3701 1108
Size: 1772 821 4260
Fragments arranged by size:
4260 1772 821
PfoI T'CCnGG_A
Cuts at: 2856 3009 3462 3780 3918 3939 4095 2856
Size: 153 453 318 138 21 156 5614
Fragments arranged by size:
5614 453 318 156 153 138 21
PmeI GTTT'AAAC
Cuts at: 4157 4157
Size: 6853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2321 2353 4922 4954 6435 6467 2321
Size: 32 2569 32 1481 32 2707
Fragments arranged by size:
2707 2569 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 3184 3517 4036 2176
Size: 1008 333 519 4993
Fragments arranged by size:
4993 1008 519 333
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6853
PsiI TTA'TAA
Cuts at: 4423 4423
Size: 6853
PspOMI G'GGCC_C
Cuts at: 2768 3294 2768
Size: 526 6327
PspXI vC'TCGA_Gb
Cuts at: 3086 3086
Size: 6853
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 6821
PstI C_TGCA'G
Cuts at: 830 2826 2925 3231 3484 3774 4205 830
Size: 1996 99 306 253 290 431 3478
Fragments arranged by size:
3478 1996 431 306 290 253 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6853
PvuII CAG'CTG
Cuts at: 2809 2833 2809
Size: 24 6829
SacI G_AGCT'C
Cuts at: 4062 4062
Size: 6853
SalI G'TCGA_C
Cuts at: 1936 4195 1936
Size: 2259 4594
SanDI GG'GwC_CC
Cuts at: 3184 3184
Size: 6853
SapI GCTCTTCn'nnn_
Cuts at: 3584 3939 3584
Size: 355 6498
SbfI CC_TGCA'GG
Cuts at: 3231 3774 4205 3231
Size: 543 431 5879
Fragments arranged by size:
5879 543 431
ScaI AGT'ACT
Cuts at: 2267 5101 6813 2267
Size: 2834 1712 2307
Fragments arranged by size:
2834 2307 1712
SfcI C'TryA_G
Cuts at: 826 1045 2822 2921 3227 3480 3770 4201 5336
Size: 219 1777 99 306 253 290 431 1135
Cuts at: 5336 6017 6208 826
Size: 681 191 1471
Fragments arranged by size:
1777 1471 1135 681 431 306 290 253
219 191 99
SfoI GGC'GCC
Cuts at: 3265 3265
Size: 6853
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6853
SmaI CCC'GGG
Cuts at: 1872 2489 4180 1872
Size: 617 1691 4545
Fragments arranged by size:
4545 1691 617
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3086 4957 5858 6120 820
Size: 197 926 1143 1871 901 262 1553
Fragments arranged by size:
1871 1553 1143 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4644 493
Size: 4151 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6853
SphI G_CATG'C
Cuts at: 1132 4211 1132
Size: 3079 3774
SspI AAT'ATT
Cuts at: 5 52 4777 5
Size: 47 4725 2081
Fragments arranged by size:
4725 2081 47
StuI AGG'CCT
Cuts at: 1517 3992 1517
Size: 2475 4378
StyI C'CwwG_G
Cuts at: 513 1065 3249 4292 513
Size: 552 2184 1043 3074
Fragments arranged by size:
3074 2184 1043 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2509 3987 4880 4897 5050 5235 5680
Size: 1255 929 1478 893 17 153 185 445
Cuts at: 5680 325
Size: 1498
Fragments arranged by size:
1498 1478 1255 929 893 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2073 2265 5099
Size: 274 80 33 51 1443 96 192 2834
Cuts at: 5099 6811 96
Size: 1712 138
Fragments arranged by size:
2834 1712 1443 274 192 138 96 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6813 6845 6813
Size: 32 6821
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6853
XbaI T'CTAG_A
Cuts at: 4189 4189
Size: 6853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2648 3256 2648
Size: 608 6245
XhoI C'TCGA_G
Cuts at: 1943 3086 1943
Size: 1143 5710
XmaI C'CCGG_G
Cuts at: 1870 2487 4178 1870
Size: 617 1691 4545
Fragments arranged by size:
4545 1691 617
XmnI GAAnn'nnTTC
Cuts at: 2120 4982 2120
Size: 2862 3991
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3199 4661 276
Size: 53 83 186 936 1665 1462 2468
Fragments arranged by size:
2468 1665 1462 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoRV FseI FspAI NsiI PacI PmlI RsrII
SacII SexAI SfiI SrfI SwaI