Restriction map for
pFN21AA0113
(Circular) (Six-base) MAPSORT of: pFN21AA0113.seq Check: 4874 from: 1 to: 6613
pFN21AA0113 6613 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2493 3037 3388 2493
Size: 544 351 5718
Fragments arranged by size:
5718 544 351
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4423 278
Size: 53 83 186 936 2887 2468
Fragments arranged by size:
2887 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3802 3956 1937
Size: 1865 154 4594
Fragments arranged by size:
4594 1865 154
Acc65I G'GTAC_C
Cuts at: 3934 3934
Size: 6613
AclI AA'CG_TT
Cuts at: 1563 4740 5113 1563
Size: 3177 373 3063
Fragments arranged by size:
3177 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 2690 3494 4676 6060 6328 1632
Size: 497 561 804 1182 1384 268 1917
Fragments arranged by size:
1917 1384 1182 804 561 497 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6416
AflIII A'CryG_T
Cuts at: 1203 5512 6489 1203
Size: 4309 977 1327
Fragments arranged by size:
4309 1327 977
AgeI A'CCGG_T
Cuts at: 5444 5444
Size: 6613
AhdI GACnn_n'nnGTC
Cuts at: 5342 5342
Size: 6613
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6613
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2492 2957 1184
Size: 1308 465 4840
Fragments arranged by size:
4840 1308 465
AlwNI CAG_nnn'CTG
Cuts at: 2312 2494 2635 2837 3028 3367 4356 5928 2312
Size: 182 141 202 191 339 989 1572 2997
Fragments arranged by size:
2997 1572 989 339 202 191 182 141
ApaI G_GGCC'C
Cuts at: 2450 3349 2450
Size: 899 5714
ApaLI G'TGCA_C
Cuts at: 2597 4670 5826 2597
Size: 2073 1156 3384
Fragments arranged by size:
3384 2073 1156
ApoI r'AATT_y
Cuts at: 1493 2098 2375 2404 2734 3922 4148 4300 6409
Size: 605 277 29 330 1188 226 152 2109
Cuts at: 6409 1493
Size: 1697
Fragments arranged by size:
2109 1697 1188 605 330 277 226 152
29
AseI AT'TA_AT
Cuts at: 160 1032 5167 160
Size: 872 4135 1606
Fragments arranged by size:
4135 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
AvaI C'yCGr_G
Cuts at: 1870 1943 3938 1870
Size: 73 1995 4545
Fragments arranged by size:
4545 1995 73
BamHI G'GATC_C
Cuts at: 3943 4310 3943
Size: 367 6246
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2363 3679 3934 5389 618
Size: 325 217 627 576 1316 255 1455 1842
Fragments arranged by size:
1842 1455 1316 627 576 325 255 217
BanII G_rGCy'C
Cuts at: 1391 2249 2271 2450 2745 3215 3332 3349 3621
Size: 858 22 179 295 470 117 17 272
Cuts at: 3621 3839 3932 1391
Size: 218 93 4072
Fragments arranged by size:
4072 858 470 295 272 218 179 117
93 22 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4804 4838 1903
Size: 34 2867 34 3678
Fragments arranged by size:
3678 2867 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4505 5715 4505
Size: 1210 5403
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6613
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5224 136
Size: 107 122 71 631 605 3552 1525
Fragments arranged by size:
3552 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3405 6608 3405
Size: 3203 3410
BlpI GC'TnA_GC
Cuts at: 2265 3209 4030 2265
Size: 944 821 4848
Fragments arranged by size:
4848 944 821
Bme1580I G_kGCm'C
Cuts at: 1790 2055 2450 2601 3349 4674 5830 1790
Size: 265 395 151 748 1325 1156 2573
Fragments arranged by size:
2573 1325 1156 748 395 265 151
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6613
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2316 4344 5302
Size: 416 53 83 399 24 899 2028 958
Cuts at: 5302 442
Size: 1753
Fragments arranged by size:
2028 1753 958 899 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6613
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3581
Size: 6613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2516 2591 2942 3080 3293 5273 1239
Size: 1277 75 351 138 213 1980 2579
Fragments arranged by size:
2579 1980 1277 351 213 138 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2729 4738 5603 5901 2729
Size: 2009 865 298 3441
Fragments arranged by size:
3441 2009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2492 3131 5276 882
Size: 617 993 639 2145 2219
Fragments arranged by size:
2219 2145 993 639 617
BsaAI yAC'GTr
Cuts at: 493 1204 4404 493
Size: 711 3200 2702
Fragments arranged by size:
3200 2702 711
BsaBI GATnn'nnATC
Cuts at: 6607 6607
Size: 6613
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3799 4420 4802 5437
Size: 53 83 186 936 2266 621 382 635
Cuts at: 5437 275
Size: 1451
Fragments arranged by size:
2266 1451 936 635 621 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2025 2976 5046 5444 5718 5865 6276 2025
Size: 951 2070 398 274 147 411 2362
Fragments arranged by size:
2362 2070 951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2284 2314 1178
Size: 30 165 30 881 30 5477
Fragments arranged by size:
5477 881 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2359 2411 2537 3095 3149 3428 3473
Size: 1024 149 52 126 558 54 279 45
Cuts at: 3473 3560 1186
Size: 87 4239
Fragments arranged by size:
4239 1024 558 279 149 126 87 54
52 45
BseYI C'CCAG_C
Cuts at: 2443 3522 5816 6312 2443
Size: 1079 2294 496 2744
Fragments arranged by size:
2744 2294 1079 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 1163
Size: 573 729 5311
Fragments arranged by size:
5311 729 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4063 4824 5852 1540
Size: 275 186 2062 761 1028 2301
Fragments arranged by size:
2301 2062 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2271 2601 2745 3747 3839 4674 4759 5830 2271
Size: 330 144 1002 92 835 85 1071 3054
Fragments arranged by size:
3054 1071 1002 835 330 144 92 85
BsmI GAATG_Cn'
Cuts at: 3533 4124 4217 3533
Size: 591 93 5929
Fragments arranged by size:
5929 591 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2249 2271 2450 2601 2745 3215
Size: 399 265 194 22 179 151 144 470
Cuts at: 3215 3332 3349 3621 3747 3839 3932 4674 4759
Size: 117 17 272 126 92 93 742 85
Cuts at: 4759 5830 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 470 399 272 265 194
179 151 144 126 117 93 92 85
22 17
BspEI T'CCGG_A
Cuts at: 6276 6276
Size: 6613
BspHI T'CATG_A
Cuts at: 4502 6232 4502
Size: 1730 4883
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2493 2855 3037 3204 3388 3968 844
Size: 1649 362 182 167 184 580 3489
Fragments arranged by size:
3489 1649 580 362 184 182 167
BsrBI CCG'CTC
Cuts at: 1394 1823 3070 4500 1394
Size: 429 1247 1430 3507
Fragments arranged by size:
3507 1430 1247 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2770 5108 5282 65
Size: 1183 1522 2338 174 1396
Fragments arranged by size:
2338 1522 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5257 5444 1700
Size: 110 3447 187 2869
Fragments arranged by size:
3447 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6613
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6613
BssSI C'ACGA_G
Cuts at: 4673 5685 4673
Size: 1012 5601
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6613
BstBI TT'CG_AA
Cuts at: 3820 5417 3820
Size: 1597 5016
BstEII G'GTnAC_C
Cuts at: 1184 3505 1184
Size: 2321 4292
BstXI CCAn_nnnn'nTGG
Cuts at: 2437 3739 2437
Size: 1302 5311
BstYI r'GATC_y
Cuts at: 1972 2028 3249 3405 3711 3943 4310 4695 4712
Size: 56 1221 156 306 232 367 385 17
Cuts at: 4712 6164 6250 6262 6608 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1221 385 367 346 306 232
156 86 56 17 12
Bsu36I CC'TnA_GG
Cuts at: 2056 2394 3353 3595 3909 2056
Size: 338 959 242 314 4760
Fragments arranged by size:
4760 959 338 314 242
BtgI C'CryG_G
Cuts at: 513 1065 2004 2533 3675 3688 3732 3738 513
Size: 552 939 529 1142 13 44 6 3388
Fragments arranged by size:
3388 1142 939 552 529 44 13 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2450 487
Size: 683 56 1224 4650
Fragments arranged by size:
4650 1224 683 56
BtsI GCAGTG_nn'
Cuts at: 2838 2941 2966 3442 4129 4923 4943 2838
Size: 103 25 476 687 794 20 4508
Fragments arranged by size:
4508 794 687 476 103 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6613
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6578
DraI TTT'AAA
Cuts at: 3917 3917
Size: 6613
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2288 5620 809
Size: 507 972 3332 1802
Fragments arranged by size:
3332 1802 972 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2536 2792 3111
Size: 54 1425 50 275 18 706 256 319
Cuts at: 3111 3735 4060 4949 6578 8
Size: 624 325 889 1629 43
Fragments arranged by size:
1629 1425 889 706 624 325 319 275
256 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4060 1537
Size: 275 2248 4090
Fragments arranged by size:
4090 2248 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2148 2549 3462 3572 3779 4543 1353
Size: 795 401 913 110 207 764 3423
Fragments arranged by size:
3423 913 795 764 401 207 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5200 5574 5720 387
Size: 1064 138 3611 374 146 1280
Fragments arranged by size:
3611 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2269 2743 3837 2269
Size: 474 1094 5045
Fragments arranged by size:
5045 1094 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2516 2591 2690 2942 3080 3293
Size: 393 497 387 75 99 252 138 213
Cuts at: 3293 3494 4676 5273 6060 6328 1239
Size: 201 1182 597 787 268 1524
Fragments arranged by size:
1524 1182 787 597 497 393 387 268
252 213 201 138 99 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2090 2090
Size: 6613
EcoO109I rG'GnC_Cy
Cuts at: 2447 2689 2872 3268 3345 3346 3642 3905 2447
Size: 242 183 396 77 1 296 263 5155
Fragments arranged by size:
5155 396 296 263 242 183 77 1
EcoRI G'AATT_C
Cuts at: 2098 2404 3922 4300 2098
Size: 306 1518 378 4411
Fragments arranged by size:
4411 1518 378 306
FspI TGC'GCA
Cuts at: 5119 5119
Size: 6613
HaeII r_GCGC'y
Cuts at: 1678 2086 2444 2494 5760 1678
Size: 408 358 50 3266 2531
Fragments arranged by size:
3266 2531 408 358 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2768 2800 3689 3721 3838
Size: 32 135 32 1196 32 889 32 117
Cuts at: 3838 3870 5254 5286 5328 5360 1373
Size: 32 1384 32 42 32 2626
Fragments arranged by size:
2626 1384 1196 889 135 117 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3957 4203 669
Size: 988 281 2019 246 3079
Fragments arranged by size:
3079 2019 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6613
HpaI GTT'AAC
Cuts at: 4203 4203
Size: 6613
KpnI G_GTAC'C
Cuts at: 3938 3938
Size: 6613
MfeI C'AATT_G
Cuts at: 4212 4212
Size: 6613
MluI A'CGCG_T
Cuts at: 6489 6489
Size: 6613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2661 5727 5911 1305
Size: 1356 3066 184 2007
Fragments arranged by size:
3066 2007 1356 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3113 3737 10
Size: 54 1425 343 1281 624 2886
Fragments arranged by size:
2886 1425 1281 624 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2430 2604 3737 4571 4930 5089 518
Size: 648 1264 174 1133 834 359 159 2042
Fragments arranged by size:
2042 1264 1133 834 648 359 174 159
MspA1I CmG'CkG
Cuts at: 1606 2701 3154 3208 3256 4029 4706 5854 6099
Size: 1095 453 54 48 773 677 1148 245
Cuts at: 6099 1606
Size: 2120
Fragments arranged by size:
2120 1148 1095 773 677 453 245 54
48
NcoI C'CATG_G
Cuts at: 513 1065 2004 3675 3688 3732 3738 513
Size: 552 939 1671 13 44 6 3388
Fragments arranged by size:
3388 1671 939 552 44 13 6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6613
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6613
NotI GC'GGCC_GC
Cuts at: 4060 4060
Size: 6613
NspI r_CATG'y
Cuts at: 1132 1229 2319 3971 5516 1132
Size: 97 1090 1652 1545 2229
Fragments arranged by size:
2229 1652 1545 1090 97
PasI CC'CwG_GG
Cuts at: 1348 2039 1348
Size: 691 5922
PciI A'CATG_T
Cuts at: 5512 5512
Size: 6613
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2360 3689 3847 1108
Size: 1252 1329 158 3874
Fragments arranged by size:
3874 1329 1252 158
PmeI GTTT'AAAC
Cuts at: 3917 3917
Size: 6613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4682 4714 6195 6227 4682
Size: 32 1481 32 5068
Fragments arranged by size:
5068 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2872 2872
Size: 6613
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6613
PsiI TTA'TAA
Cuts at: 4183 4183
Size: 6613
PspOMI G'GGCC_C
Cuts at: 2446 3345 2446
Size: 899 5714
PstI C_TGCA'G
Cuts at: 830 2490 2631 3201 3498 3965 830
Size: 1660 141 570 297 467 3478
Fragments arranged by size:
3478 1660 570 467 297 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6613
PvuII CAG'CTG
Cuts at: 3154 3208 3154
Size: 54 6559
SacI G_AGCT'C
Cuts at: 2271 2745 3839 2271
Size: 474 1094 5045
Fragments arranged by size:
5045 1094 474
SalI G'TCGA_C
Cuts at: 1936 3955 1936
Size: 2019 4594
SapI GCTCTTCn'nnn_
Cuts at: 2549 3462 2549
Size: 913 5700
SbfI CC_TGCA'GG
Cuts at: 3201 3965 3201
Size: 764 5849
ScaI AGT'ACT
Cuts at: 4861 6573 4861
Size: 1712 4901
SfcI C'TryA_G
Cuts at: 826 1045 2486 2627 3197 3494 3870 3961 5096
Size: 219 1441 141 570 297 376 91 1135
Cuts at: 5096 5777 5968 826
Size: 681 191 1471
Fragments arranged by size:
1471 1441 1135 681 570 376 297 219
191 141 91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6613
SmaI CCC'GGG
Cuts at: 1872 3940 1872
Size: 2068 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2744 4717 5618 5880 820
Size: 197 926 801 1973 901 262 1553
Fragments arranged by size:
1973 1553 926 901 801 262 197
SnaBI TAC'GTA
Cuts at: 493 4404 493
Size: 3911 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6613
SphI G_CATG'C
Cuts at: 1132 2319 3971 1132
Size: 1187 1652 3774
Fragments arranged by size:
3774 1652 1187
SspI AAT'ATT
Cuts at: 5 52 4537 5
Size: 47 4485 2081
Fragments arranged by size:
4485 2081 47
StuI AGG'CCT
Cuts at: 1517 3593 3868 1517
Size: 2076 275 4262
Fragments arranged by size:
4262 2076 275
StyI C'CwwG_G
Cuts at: 513 1065 2004 2241 2592 2889 3168 3675 3688
Size: 552 939 237 351 297 279 507 13
Cuts at: 3688 3732 3738 4052 513
Size: 44 6 314 3074
Fragments arranged by size:
3074 939 552 507 351 314 297 279
237 44 13 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2564 3155 3639 4640 4657 4810 4995
Size: 1255 984 591 484 1001 17 153 185
Cuts at: 4995 5440 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1001 984 591 484 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4859 6571 96
Size: 274 80 33 51 1443 2882 1712 138
Fragments arranged by size:
2882 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2284 2316 3273 3305 6573 6605 2284
Size: 32 957 32 3268 32 2292
Fragments arranged by size:
3268 2292 957 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6613
XbaI T'CTAG_A
Cuts at: 3949 3949
Size: 6613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3127 3673 3127
Size: 546 6067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6613
XmaI C'CCGG_G
Cuts at: 1870 3938 1870
Size: 2068 4545
XmnI GAAnn'nnTTC
Cuts at: 3407 4742 3407
Size: 1335 5278
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4421 276
Size: 53 83 186 936 2887 2468
Fragments arranged by size:
2887 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI