Restriction map for
pFN21AA0110
(Circular) (Six-base) MAPSORT of: pFN21AA0110.seq Check: 7280 from: 1 to: 5527
pFN21AA0110 5527 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3337 278
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2870 1937
Size: 933 4594
Acc65I G'GTAC_C
Cuts at: 2170 2848 2170
Size: 678 4849
AclI AA'CG_TT
Cuts at: 1563 3654 4027 1563
Size: 2091 373 3063
Fragments arranged by size:
3063 2091 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2351 2597 3590 4974 5242 1632
Size: 719 246 993 1384 268 1917
Fragments arranged by size:
1917 1384 993 719 268 246
AflII C'TTAA_G
Cuts at: 820 1017 2285 820
Size: 197 1268 4062
Fragments arranged by size:
4062 1268 197
AflIII A'CryG_T
Cuts at: 1203 4426 5403 1203
Size: 3223 977 1327
Fragments arranged by size:
3223 1327 977
AgeI A'CCGG_T
Cuts at: 4358 4358
Size: 5527
AhdI GACnn_n'nnGTC
Cuts at: 4256 4256
Size: 5527
AleI CACnn'nnGTG
Cuts at: 1166 2357 1166
Size: 1191 4336
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5527
AlwNI CAG_nnn'CTG
Cuts at: 2048 2545 3270 4842 2048
Size: 497 725 1572 2733
Fragments arranged by size:
2733 1572 725 497
ApaLI G'TGCA_C
Cuts at: 3584 4740 3584
Size: 1156 4371
ApoI r'AATT_y
Cuts at: 1493 2836 3062 3214 5323 1493
Size: 1343 226 152 2109 1697
Fragments arranged by size:
2109 1697 1343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4081 160
Size: 872 3049 1606
Fragments arranged by size:
3049 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
AvaI C'yCGr_G
Cuts at: 1870 1943 2343 2852 1870
Size: 73 400 509 4545
Fragments arranged by size:
4545 509 400 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2427 2460 2427
Size: 33 5494
BamHI G'GATC_C
Cuts at: 2857 3224 2857
Size: 367 5160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2170 2633 2797 2848
Size: 325 217 627 224 159 463 164 51
Cuts at: 2848 4303 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 463 325 224 217 164
159 51
BanII G_rGCy'C
Cuts at: 1391 2846 1391
Size: 1455 4072
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 5527
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2784 928
Size: 971 885 3671
Fragments arranged by size:
3671 971 885
BbvCI CC'TCA_GC
Cuts at: 2035 2408 2035
Size: 373 5154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3718 3752 1903
Size: 34 1781 34 3678
Fragments arranged by size:
3678 1781 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3419 4629 3419
Size: 1210 4317
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2205 4138
Size: 107 122 71 631 605 337 196 1933
Cuts at: 4138 136
Size: 1525
Fragments arranged by size:
1933 1525 631 605 337 196 122 107
71
BglII A'GATC_T
Cuts at: 5522 5522
Size: 5527
BlpI GC'TnA_GC
Cuts at: 2944 2944
Size: 5527
Bme1580I G_kGCm'C
Cuts at: 1790 2636 2709 3588 4744 1790
Size: 846 73 879 1156 2573
Fragments arranged by size:
2573 1156 879 846 73
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5527
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3258 4216 442
Size: 416 53 83 399 24 1841 958 1753
Fragments arranged by size:
1841 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5527
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 5527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 2438 4187 1239
Size: 1178 21 1749 2579
Fragments arranged by size:
2579 1749 1178 21
Bpu10I CC'TnA_GC
Cuts at: 2035 2408 2480 2546 2035
Size: 373 72 66 5016
Fragments arranged by size:
5016 373 72 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 3652 4517 4815 2126
Size: 1526 865 298 2838
Fragments arranged by size:
2838 1526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4190 882
Size: 617 2691 2219
Fragments arranged by size:
2691 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3318 493
Size: 711 2114 2702
Fragments arranged by size:
2702 2114 711
BsaBI GATnn'nnATC
Cuts at: 5521 5521
Size: 5527
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3334 3716 4351
Size: 53 83 186 936 479 1322 382 635
Cuts at: 4351 275
Size: 1451
Fragments arranged by size:
1451 1322 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3960 4358 4632 4779 5190 3960
Size: 398 274 147 411 4297
Fragments arranged by size:
4297 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2015 2045 1178
Size: 30 165 30 612 30 4660
Fragments arranged by size:
4660 612 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 2610 1186
Size: 1150 274 4103
Fragments arranged by size:
4103 1150 274
BseYI C'CCAG_C
Cuts at: 2708 4730 5226 2708
Size: 2022 496 3009
Fragments arranged by size:
3009 2022 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4954
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2977 3738 4766 1540
Size: 275 186 976 761 1028 2301
Fragments arranged by size:
2301 1028 976 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2552 3588 3673 4744 2552
Size: 1036 85 1071 3335
Fragments arranged by size:
3335 1071 1036 85
BsmI GAATG_Cn'
Cuts at: 3038 3131 3038
Size: 93 5434
BsmBI CGTCTCn'nnnn_
Cuts at: 2576 2576
Size: 5527
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2552 2636 2709 2846 3588 3673 4744
Size: 399 762 84 73 137 742 85 1071
Cuts at: 4744 1391
Size: 2174
Fragments arranged by size:
2174 1071 762 742 399 137 85 84
73
BspEI T'CCGG_A
Cuts at: 5190 5190
Size: 5527
BspHI T'CATG_A
Cuts at: 3416 5146 3416
Size: 1730 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2882 844
Size: 2038 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3414 1394
Size: 429 1591 3507
Fragments arranged by size:
3507 1591 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2479 4022 4196 65
Size: 1183 1231 1543 174 1396
Fragments arranged by size:
1543 1396 1231 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4171 4358 1700
Size: 110 2361 187 2869
Fragments arranged by size:
2869 2361 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5527
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5527
BssSI C'ACGA_G
Cuts at: 3587 4599 3587
Size: 1012 4515
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2373 794
Size: 1579 3948
BstBI TT'CG_AA
Cuts at: 4331 4331
Size: 5527
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5527
BstXI CCAn_nnnn'nTGG
Cuts at: 2590 2590
Size: 5527
BstYI r'GATC_y
Cuts at: 1972 2857 3224 3609 3626 5078 5164 5176 5522
Size: 885 367 385 17 1452 86 12 346
Cuts at: 5522 1972
Size: 1977
Fragments arranged by size:
1977 1452 885 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2766 513
Size: 552 939 762 3274
Fragments arranged by size:
3274 939 762 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4788
Fragments arranged by size:
4788 683 56
BtsI GCAGTG_nn'
Cuts at: 3043 3837 3857 3043
Size: 794 20 4713
Fragments arranged by size:
4713 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5527
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5492
DraI TTT'AAA
Cuts at: 2812 2831 2812
Size: 19 5508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4534 809
Size: 507 3218 1802
Fragments arranged by size:
3218 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2974 3863 5492
Size: 54 1425 50 275 18 1144 889 1629
Cuts at: 5492 8
Size: 43
Fragments arranged by size:
1629 1425 1144 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2974 1537
Size: 275 1162 4090
Fragments arranged by size:
4090 1162 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2579 3457 1353
Size: 1226 878 3423
Fragments arranged by size:
3423 1226 878
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2036 4114 4488 4634 387
Size: 1064 138 447 2078 374 146 1280
Fragments arranged by size:
2078 1280 1064 447 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2351 2417 2438 2597 3590 4187 4974
Size: 393 719 66 21 159 993 597 787
Cuts at: 4974 5242 1239
Size: 268 1524
Fragments arranged by size:
1524 993 787 719 597 393 268 159
66 21
EcoRI G'AATT_C
Cuts at: 2836 3214 2836
Size: 378 5149
FspI TGC'GCA
Cuts at: 4033 4033
Size: 5527
HaeII r_GCGC'y
Cuts at: 1678 2015 4674 1678
Size: 337 2659 2531
Fragments arranged by size:
2659 2531 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2676 2708 2742
Size: 32 135 32 443 32 629 32 34
Cuts at: 2742 2774 4168 4200 4242 4274 1373
Size: 32 1394 32 42 32 2626
Fragments arranged by size:
2626 1394 629 443 135 42 34 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2871 3117 669
Size: 988 281 933 246 3079
Fragments arranged by size:
3079 988 933 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5527
HpaI GTT'AAC
Cuts at: 3117 3117
Size: 5527
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 5527
KpnI G_GTAC'C
Cuts at: 2174 2852 2174
Size: 678 4849
MfeI C'AATT_G
Cuts at: 3126 3126
Size: 5527
MluI A'CGCG_T
Cuts at: 5403 5403
Size: 5527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4641 4825 1305
Size: 3336 184 2007
Fragments arranged by size:
3336 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3705
Fragments arranged by size:
3705 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2588 3485 3844 4003 518
Size: 648 1191 231 897 359 159 2042
Fragments arranged by size:
2042 1191 897 648 359 231 159
MspA1I CmG'CkG
Cuts at: 1606 2943 3620 4768 5013 1606
Size: 1337 677 1148 245 2120
Fragments arranged by size:
2120 1337 1148 677 245
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 5527
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4036
Fragments arranged by size:
4036 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5527
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5527
NotI GC'GGCC_GC
Cuts at: 2974 2974
Size: 5527
NspI r_CATG'y
Cuts at: 1132 1229 2885 4430 1132
Size: 97 1656 1545 2229
Fragments arranged by size:
2229 1656 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5527
PciI A'CATG_T
Cuts at: 4426 4426
Size: 5527
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2772 1108
Size: 1664 3863
PmeI GTTT'AAAC
Cuts at: 2831 2831
Size: 5527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 5109 5141 3596
Size: 32 1481 32 3982
Fragments arranged by size:
3982 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5527
PsiI TTA'TAA
Cuts at: 3097 3097
Size: 5527
PstI C_TGCA'G
Cuts at: 830 2879 830
Size: 2049 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5527
SalI G'TCGA_C
Cuts at: 1936 2869 1936
Size: 933 4594
SbfI CC_TGCA'GG
Cuts at: 2879 2879
Size: 5527
ScaI AGT'ACT
Cuts at: 3775 5487 3775
Size: 1712 3815
SfcI C'TryA_G
Cuts at: 826 1045 2527 2875 4010 4691 4882 826
Size: 219 1482 348 1135 681 191 1471
Fragments arranged by size:
1482 1471 1135 681 348 219 191
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 5527
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5527
SmaI CCC'GGG
Cuts at: 1872 2854 1872
Size: 982 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 2285 3631 4532 4794 820
Size: 197 926 162 180 1346 901 262 1553
Fragments arranged by size:
1553 1346 926 901 262 197 180 162
SnaBI TAC'GTA
Cuts at: 493 3318 493
Size: 2825 2702
SpeI A'CTAG_T
Cuts at: 152 2440 152
Size: 2288 3239
SphI G_CATG'C
Cuts at: 1132 2885 1132
Size: 1753 3774
SspI AAT'ATT
Cuts at: 5 52 3451 5
Size: 47 3399 2081
Fragments arranged by size:
3399 2081 47
StuI AGG'CCT
Cuts at: 1517 2147 1517
Size: 630 4897
StyI C'CwwG_G
Cuts at: 513 1065 2004 2464 2487 2966 513
Size: 552 939 460 23 479 3074
Fragments arranged by size:
3074 939 552 479 460 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3554 3571 3724 3909 4354 325
Size: 1255 1974 17 153 185 445 1498
Fragments arranged by size:
1974 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3773 5485 96
Size: 274 80 33 51 1443 1796 1712 138
Fragments arranged by size:
1796 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5487 5519 5487
Size: 32 5495
Tth111I GACn'n_nGTC
Cuts at: 1316 2737 1316
Size: 1421 4106
XbaI T'CTAG_A
Cuts at: 2863 2863
Size: 5527
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5527
XmaI C'CCGG_G
Cuts at: 1870 2852 1870
Size: 982 4545
XmnI GAAnn'nnTTC
Cuts at: 2305 3656 2305
Size: 1351 4176
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3335 276
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI