Restriction map for
pFN21AA0109
(Circular) (Six-base) MAPSORT of: pFN21AA0109.seq Check: 7625 from: 1 to: 6010
pFN21AA0109 6010 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2119 2563 2995 2119
Size: 444 432 5134
Fragments arranged by size:
5134 444 432
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3820 278
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2061 3353 1937
Size: 124 1292 4594
Fragments arranged by size:
4594 1292 124
Acc65I G'GTAC_C
Cuts at: 3331 3331
Size: 6010
AclI AA'CG_TT
Cuts at: 1563 4137 4510 1563
Size: 2574 373 3063
Fragments arranged by size:
3063 2574 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4073 5457 5725 1632
Size: 2441 1384 268 1917
Fragments arranged by size:
2441 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 4909 5886 1203
Size: 3706 977 1327
Fragments arranged by size:
3706 1327 977
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 4739 4739
Size: 6010
AleI CACnn'nnGTG
Cuts at: 1166 2754 1166
Size: 1588 4422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2742 1184
Size: 1558 4452
AlwNI CAG_nnn'CTG
Cuts at: 2578 3753 5325 2578
Size: 1175 1572 3263
Fragments arranged by size:
3263 1572 1175
ApaLI G'TGCA_C
Cuts at: 4067 5223 4067
Size: 1156 4854
ApoI r'AATT_y
Cuts at: 1493 2243 2371 3319 3545 3697 5806 1493
Size: 750 128 948 226 152 2109 1697
Fragments arranged by size:
2109 1697 948 750 226 152 128
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 3335 1870
Size: 73 112 1280 4545
Fragments arranged by size:
4545 1280 112 73
BamHI G'GATC_C
Cuts at: 2973 3340 3707 2973
Size: 367 367 5276
Fragments arranged by size:
5276 367 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3178 3331 4786 618
Size: 325 217 627 1391 153 1455 1842
Fragments arranged by size:
1842 1455 1391 627 325 217 153
BanII G_rGCy'C
Cuts at: 1391 2523 3151 3329 1391
Size: 1132 628 178 4072
Fragments arranged by size:
4072 1132 628 178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2230 928
Size: 971 331 4708
Fragments arranged by size:
4708 971 331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4201 4235 1903
Size: 34 2264 34 3678
Fragments arranged by size:
3678 2264 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3902 5112 3902
Size: 1210 4800
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6010
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3288 4621 136
Size: 107 122 71 631 605 1616 1333 1525
Fragments arranged by size:
1616 1525 1333 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6005 6005
Size: 6010
BlpI GC'TnA_GC
Cuts at: 3427 3427
Size: 6010
Bme1580I G_kGCm'C
Cuts at: 1790 2587 4071 5227 1790
Size: 797 1484 1156 2573
Fragments arranged by size:
2573 1484 1156 797
BmgBI CAC'GTC
Cuts at: 1318 2266 1318
Size: 948 5062
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2481 3741 4699
Size: 416 53 83 399 24 1064 1260 958
Cuts at: 4699 442
Size: 1753
Fragments arranged by size:
1753 1260 1064 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2558 2927 4670 1239
Size: 1319 369 1743 2579
Fragments arranged by size:
2579 1743 1319 369
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3185 4135 5000 5298 3185
Size: 950 865 298 3897
Fragments arranged by size:
3897 950 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4673 882
Size: 617 3174 2219
Fragments arranged by size:
3174 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3801 493
Size: 711 2597 2702
Fragments arranged by size:
2702 2597 711
BsaBI GATnn'nnATC
Cuts at: 6004 6004
Size: 6010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3817 4199 4834 275
Size: 53 83 186 936 2284 382 635 1451
Fragments arranged by size:
2284 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4443 4841 5115 5262 5673 4443
Size: 398 274 147 411 4780
Fragments arranged by size:
4780 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5785
Fragments arranged by size:
5785 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6010
BseYI C'CCAG_C
Cuts at: 5213 5709 5213
Size: 496 5514
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2725 3023 1163
Size: 573 989 298 4150
Fragments arranged by size:
4150 989 573 298
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3460 4221 5249 1540
Size: 275 186 1459 761 1028 2301
Fragments arranged by size:
2301 1459 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2049 2325 2523 4071 4156 5227 2049
Size: 276 198 1548 85 1071 2832
Fragments arranged by size:
2832 1548 1071 276 198 85
BsmI GAATG_Cn'
Cuts at: 2642 3055 3521 3614 2642
Size: 413 466 93 5038
Fragments arranged by size:
5038 466 413 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2325 2523 2587 3151 3329 4071
Size: 399 259 276 198 64 564 178 742
Cuts at: 4071 4156 5227 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 564 399 276 259 198
178 85 64
BspEI T'CCGG_A
Cuts at: 5673 5673
Size: 6010
BspHI T'CATG_A
Cuts at: 3899 5629 3899
Size: 1730 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2119 2559 2563 2995 3365 844
Size: 1275 440 4 432 370 3489
Fragments arranged by size:
3489 1275 440 432 370 4
BsrBI CCG'CTC
Cuts at: 1394 1823 2712 3897 1394
Size: 429 889 1185 3507
Fragments arranged by size:
3507 1185 889 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2151 4505 4679 65
Size: 1183 903 2354 174 1396
Fragments arranged by size:
2354 1396 1183 903 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4654 4841 1700
Size: 110 2844 187 2869
Fragments arranged by size:
2869 2844 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6010
BssHII G'CGCG_C
Cuts at: 1925 2385 1925
Size: 460 5550
BssSI C'ACGA_G
Cuts at: 4070 5082 4070
Size: 1012 4998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2093 2131 794
Size: 1299 38 4673
Fragments arranged by size:
4673 1299 38
BstBI TT'CG_AA
Cuts at: 2241 4814 2241
Size: 2573 3437
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6010
BstXI CCAn_nnnn'nTGG
Cuts at: 2471 2471
Size: 6010
BstYI r'GATC_y
Cuts at: 1972 2973 3340 3707 4092 4109 5561 5647 5659
Size: 1001 367 367 385 17 1452 86 12
Cuts at: 5659 6005 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1001 385 367 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2232 513
Size: 552 1167 4291
Fragments arranged by size:
4291 1167 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5271
Fragments arranged by size:
5271 683 56
BtsI GCAGTG_nn'
Cuts at: 2094 2945 3292 3526 4320 4340 2094
Size: 851 347 234 794 20 3764
Fragments arranged by size:
3764 851 794 347 234 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 2938 3314 2938
Size: 376 5634
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3254 5017 809
Size: 507 1938 1763 1802
Fragments arranged by size:
1938 1802 1763 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3280 3457 4346
Size: 54 1425 50 275 18 1450 177 889
Cuts at: 4346 5975 8
Size: 1629 43
Fragments arranged by size:
1629 1450 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3457 1537
Size: 275 1645 4090
Fragments arranged by size:
4090 1645 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2436 2528 2606 3940 1353
Size: 1083 92 78 1334 3423
Fragments arranged by size:
3423 1334 1083 92 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4597 4971 5117 387
Size: 1064 138 3008 374 146 1280
Fragments arranged by size:
3008 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2521 2521
Size: 6010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2558 2927 4073 4670 5457 5725 1239
Size: 393 926 369 1146 597 787 268 1524
Fragments arranged by size:
1524 1146 926 787 597 393 369 268
EcoRI G'AATT_C
Cuts at: 2243 2371 3319 3697 2243
Size: 128 948 378 4556
Fragments arranged by size:
4556 948 378 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 6010
FspI TGC'GCA
Cuts at: 2135 4516 2135
Size: 2381 3629
HaeII r_GCGC'y
Cuts at: 1678 5157 1678
Size: 3479 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 2413 2445 2484
Size: 32 135 32 486 32 323 32 39
Cuts at: 2484 2516 4651 4683 4725 4757 1373
Size: 32 2135 32 42 32 2626
Fragments arranged by size:
2626 2135 486 323 135 42 39 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2224 3354 3600 669
Size: 988 281 286 1130 246 3079
Fragments arranged by size:
3079 1130 988 286 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6010
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KpnI G_GTAC'C
Cuts at: 3335 3335
Size: 6010
MfeI C'AATT_G
Cuts at: 2724 3609 2724
Size: 885 5125
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 2492 2955 5124 5308 1305
Size: 906 281 463 2169 184 2007
Fragments arranged by size:
2169 2007 906 463 281 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4188
Fragments arranged by size:
4188 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2754 3164 3968 4327 4486 518
Size: 648 1588 410 804 359 159 2042
Fragments arranged by size:
2042 1588 804 648 410 359 159
MspA1I CmG'CkG
Cuts at: 1606 2693 3426 4103 5251 5496 1606
Size: 1087 733 677 1148 245 2120
Fragments arranged by size:
2120 1148 1087 733 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2232 513
Size: 552 1167 4291
Fragments arranged by size:
4291 1167 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 2632 3368 4913 1132
Size: 97 1403 736 1545 2229
Fragments arranged by size:
2229 1545 1403 736 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6010
PciI A'CATG_T
Cuts at: 4909 4909
Size: 6010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2193 3060 1108
Size: 1085 867 4058
Fragments arranged by size:
4058 1085 867
PfoI T'CCnGG_A
Cuts at: 2526 2526
Size: 6010
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6010
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 6010
PstI C_TGCA'G
Cuts at: 830 3362 830
Size: 2532 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
PvuII CAG'CTG
Cuts at: 2693 2693
Size: 6010
SacI G_AGCT'C
Cuts at: 2523 2523
Size: 6010
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SapI GCTCTTCn'nnn_
Cuts at: 2436 2528 2606 2436
Size: 92 78 5840
Fragments arranged by size:
5840 92 78
SbfI CC_TGCA'GG
Cuts at: 3362 3362
Size: 6010
ScaI AGT'ACT
Cuts at: 4258 5970 4258
Size: 1712 4298
SfcI C'TryA_G
Cuts at: 826 1045 3241 3358 4493 5174 5365 826
Size: 219 2196 117 1135 681 191 1471
Fragments arranged by size:
2196 1471 1135 681 219 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6010
SmaI CCC'GGG
Cuts at: 1872 3337 1872
Size: 1465 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3200 4114 5015 5277 820
Size: 197 926 1257 914 901 262 1553
Fragments arranged by size:
1553 1257 926 914 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 2632 3368 1132
Size: 1500 736 3774
Fragments arranged by size:
3774 1500 736
SspI AAT'ATT
Cuts at: 5 52 3934 5
Size: 47 3882 2081
Fragments arranged by size:
3882 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6010
StyI C'CwwG_G
Cuts at: 513 1065 2232 2844 3449 513
Size: 552 1167 612 605 3074
Fragments arranged by size:
3074 1167 612 605 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3256 4037 4054 4207 4392 4837 325
Size: 1255 1676 781 17 153 185 445 1498
Fragments arranged by size:
1676 1498 1255 781 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3033 4256 5968
Size: 274 80 33 51 1443 1056 1223 1712
Cuts at: 5968 96
Size: 138
Fragments arranged by size:
1712 1443 1223 1056 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5970 6002 5970
Size: 32 5978
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6010
XbaI T'CTAG_A
Cuts at: 2350 3346 2350
Size: 996 5014
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2194 2194
Size: 6010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6010
XmaI C'CCGG_G
Cuts at: 1870 3335 1870
Size: 1465 4545
XmnI GAAnn'nnTTC
Cuts at: 2396 4139 2396
Size: 1743 4267
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3818 276
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI