Restriction map for
pFN21AA0107
(Circular) (Six-base) MAPSORT of: pFN21AA0107.seq Check: 8268 from: 1 to: 5872
pFN21AA0107 5872 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3215 1937
Size: 1278 4594
Acc65I G'GTAC_C
Cuts at: 3193 3193
Size: 5872
AclI AA'CG_TT
Cuts at: 1563 3999 4372 1563
Size: 2436 373 3063
Fragments arranged by size:
3063 2436 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3935 5319 5587 1632
Size: 2303 1384 268 1917
Fragments arranged by size:
2303 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5675
AflIII A'CryG_T
Cuts at: 1203 4771 5748 1203
Size: 3568 977 1327
Fragments arranged by size:
3568 1327 977
AgeI A'CCGG_T
Cuts at: 4703 4703
Size: 5872
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5872
AlwNI CAG_nnn'CTG
Cuts at: 2782 2926 3004 3615 5187 2782
Size: 144 78 611 1572 3467
Fragments arranged by size:
3467 1572 611 144 78
ApaLI G'TGCA_C
Cuts at: 3929 5085 3929
Size: 1156 4716
ApoI r'AATT_y
Cuts at: 1493 2301 2904 2987 3181 3407 3559 5668 1493
Size: 808 603 83 194 226 152 2109 1697
Fragments arranged by size:
2109 1697 808 603 226 194 152 83
AseI AT'TA_AT
Cuts at: 160 1032 3165 4426 160
Size: 872 2133 1261 1606
Fragments arranged by size:
2133 1606 1261 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 3197 1870
Size: 73 147 1107 4545
Fragments arranged by size:
4545 1107 147 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2041 2074 2041
Size: 33 5839
BamHI G'GATC_C
Cuts at: 3202 3569 3202
Size: 367 5505
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3193 4648 618
Size: 325 217 627 1406 1455 1842
Fragments arranged by size:
1842 1455 1406 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2365 3191 1391
Size: 974 826 4072
Fragments arranged by size:
4072 974 826
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2753 928
Size: 971 854 4047
Fragments arranged by size:
4047 971 854
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2167 2201 4063 4097 1903
Size: 34 50 34 146 34 1862 34 3678
Fragments arranged by size:
3678 1862 146 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3764 4974 3764
Size: 1210 4662
BclI T'GATC_A
Cuts at: 1548 2995 1548
Size: 1447 4425
BfrBI ATG'CAT
Cuts at: 2912 2912
Size: 5872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4483 136
Size: 107 122 71 631 605 2811 1525
Fragments arranged by size:
2811 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2707 3124 5867 2707
Size: 417 2743 2712
Fragments arranged by size:
2743 2712 417
BlpI GC'TnA_GC
Cuts at: 2081 3289 2081
Size: 1208 4664
Bme1580I G_kGCm'C
Cuts at: 1790 3933 5089 1790
Size: 2143 1156 2573
Fragments arranged by size:
2573 2143 1156
BmgBI CAC'GTC
Cuts at: 1318 2203 1318
Size: 885 4987
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2533 3603 4561
Size: 416 53 83 399 24 1116 1070 958
Cuts at: 4561 442
Size: 1753
Fragments arranged by size:
1753 1116 1070 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2042 4532 1239
Size: 794 9 2490 2579
Fragments arranged by size:
2579 2490 794 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3997 4862 5160 3997
Size: 865 298 4709
Fragments arranged by size:
4709 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4535 882
Size: 617 3036 2219
Fragments arranged by size:
3036 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3663 493
Size: 711 2459 2702
Fragments arranged by size:
2702 2459 711
BsaBI GATnn'nnATC
Cuts at: 2466 5866 2466
Size: 3400 2472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3679 4061 4696 275
Size: 53 83 186 936 2146 382 635 1451
Fragments arranged by size:
2146 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4305 4703 4977 5124 5535 4305
Size: 398 274 147 411 4642
Fragments arranged by size:
4642 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5647
Fragments arranged by size:
5647 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2042 2276 2532 1186
Size: 853 3 234 256 4526
Fragments arranged by size:
4526 853 256 234 3
BseYI C'CCAG_C
Cuts at: 5075 5571 5075
Size: 496 5376
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5299
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2136 3322 4083 5111 1540
Size: 275 186 135 1186 761 1028 2301
Fragments arranged by size:
2301 1186 1028 761 275 186 135
BsiHKAI G_wGCw'C
Cuts at: 2097 2365 3933 4018 5089 2097
Size: 268 1568 85 1071 2880
Fragments arranged by size:
2880 1568 1071 268 85
BsmI GAATG_Cn'
Cuts at: 2804 3383 3476 2804
Size: 579 93 5200
Fragments arranged by size:
5200 579 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2097 2097
Size: 5872
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2365 3191 3933 4018 5089 1391
Size: 399 307 268 826 742 85 1071 2174
Fragments arranged by size:
2174 1071 826 742 399 307 268 85
BspEI T'CCGG_A
Cuts at: 5535 5535
Size: 5872
BspHI T'CATG_A
Cuts at: 3761 5491 3761
Size: 1730 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3227 844
Size: 2383 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3759 1394
Size: 429 1936 3507
Fragments arranged by size:
3507 1936 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2319 4367 4541 65
Size: 1183 1071 2048 174 1396
Fragments arranged by size:
2048 1396 1183 1071 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4516 4703 1700
Size: 110 2706 187 2869
Fragments arranged by size:
2869 2706 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5872
BssSI C'ACGA_G
Cuts at: 3932 4944 3932
Size: 1012 4860
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5872
BstBI TT'CG_AA
Cuts at: 4676 4676
Size: 5872
BstEII G'GTnAC_C
Cuts at: 1184 2408 1184
Size: 1224 4648
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5872
BstYI r'GATC_y
Cuts at: 1972 2707 3124 3202 3569 3954 3971 5423 5509
Size: 735 417 78 367 385 17 1452 86
Cuts at: 5509 5521 5867 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 735 417 385 367 346 86
78 17 12
Bsu36I CC'TnA_GG
Cuts at: 2438 2438
Size: 5872
BtgI C'CryG_G
Cuts at: 513 1065 2098 513
Size: 552 1033 4287
Fragments arranged by size:
4287 1033 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2322 487
Size: 683 56 1096 4037
Fragments arranged by size:
4037 1096 683 56
BtsI GCAGTG_nn'
Cuts at: 3034 3388 4182 4202 3034
Size: 354 794 20 4704
Fragments arranged by size:
4704 794 354 20
ClaI AT'CG_AT
Cuts at: 1554 2881 1554
Size: 1327 4545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5837
DraI TTT'AAA
Cuts at: 3176 3176
Size: 5872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4879 809
Size: 507 3563 1802
Fragments arranged by size:
3563 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 3319 4208
Size: 54 1425 50 275 18 303 1186 889
Cuts at: 4208 5837 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1186 889 303 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2133 3319 1537
Size: 275 321 1186 4090
Fragments arranged by size:
4090 1186 321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2609 3802 1353
Size: 1256 1193 3423
Fragments arranged by size:
3423 1256 1193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4459 4833 4979 387
Size: 1064 138 2870 374 146 1280
Fragments arranged by size:
2870 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2363 2363
Size: 5872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2042 3935 4532 5319 5587 1239
Size: 393 401 9 1893 597 787 268 1524
Fragments arranged by size:
1893 1524 787 597 401 393 268 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2024 2024
Size: 5872
EcoRI G'AATT_C
Cuts at: 2904 2987 3181 3559 2904
Size: 83 194 378 5217
Fragments arranged by size:
5217 378 194 83
FspI TGC'GCA
Cuts at: 4378 4378
Size: 5872
HaeII r_GCGC'y
Cuts at: 1678 5019 1678
Size: 3341 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2694 2726 4513 4545 4587
Size: 32 135 32 1122 32 1787 32 42
Cuts at: 4587 4619 1373
Size: 32 2626
Fragments arranged by size:
2626 1787 1122 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3216 3462 669
Size: 988 281 1278 246 3079
Fragments arranged by size:
3079 1278 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2500 748
Size: 1752 4120
HpaI GTT'AAC
Cuts at: 3462 3462
Size: 5872
KpnI G_GTAC'C
Cuts at: 3197 3197
Size: 5872
MfeI C'AATT_G
Cuts at: 3471 3471
Size: 5872
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4986 5170 1305
Size: 3681 184 2007
Fragments arranged by size:
3681 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4050
Fragments arranged by size:
4050 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3830 4189 4348 518
Size: 648 2664 359 159 2042
Fragments arranged by size:
2664 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2068 2100 2597 2923 3288 3965 5113
Size: 405 57 32 497 326 365 677 1148
Cuts at: 5113 5358 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 497 405 365 326 245
57 32
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5320
NdeI CA'TA_TG
Cuts at: 387 2386 387
Size: 1999 3873
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5872
NotI GC'GGCC_GC
Cuts at: 3319 3319
Size: 5872
NruI TCG'CGA
Cuts at: 2307 2307
Size: 5872
NsiI A_TGCA'T
Cuts at: 2914 2914
Size: 5872
NspI r_CATG'y
Cuts at: 1132 1229 3230 4775 1132
Size: 97 2001 1545 2229
Fragments arranged by size:
2229 2001 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2404 1348
Size: 1056 4816
PciI A'CATG_T
Cuts at: 4771 4771
Size: 5872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5872
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 5454 5486 3941
Size: 32 1481 32 4327
Fragments arranged by size:
4327 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5872
PsiI TTA'TAA
Cuts at: 2652 3442 2652
Size: 790 5082
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3083 3115 3083
Size: 32 5840
PstI C_TGCA'G
Cuts at: 830 3224 830
Size: 2394 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
PvuII CAG'CTG
Cuts at: 2068 2597 2923 2068
Size: 529 326 5017
Fragments arranged by size:
5017 529 326
SacI G_AGCT'C
Cuts at: 2365 2365
Size: 5872
SacII CC_GC'GG
Cuts at: 2101 2101
Size: 5872
SalI G'TCGA_C
Cuts at: 1936 3214 1936
Size: 1278 4594
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 4120 5832 4120
Size: 1712 4160
SfcI C'TryA_G
Cuts at: 826 1045 3220 4355 5036 5227 826
Size: 219 2175 1135 681 191 1471
Fragments arranged by size:
2175 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5872
SmaI CCC'GGG
Cuts at: 1872 3199 1872
Size: 1327 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3976 4877 5139 820
Size: 197 926 2033 901 262 1553
Fragments arranged by size:
2033 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5872
SphI G_CATG'C
Cuts at: 1132 3230 1132
Size: 2098 3774
SspI AAT'ATT
Cuts at: 5 52 3796 5
Size: 47 3744 2081
Fragments arranged by size:
3744 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5872
StyI C'CwwG_G
Cuts at: 513 1065 2950 3311 513
Size: 552 1885 361 3074
Fragments arranged by size:
3074 1885 552 361
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3899 3916 4069 4254 4699 325
Size: 1255 2319 17 153 185 445 1498
Fragments arranged by size:
2319 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4118 5830 96
Size: 274 80 33 51 1443 2141 1712 138
Fragments arranged by size:
2141 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5832 5864 5832
Size: 32 5840
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5872
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5872
XmaI C'CCGG_G
Cuts at: 1870 3197 1870
Size: 1327 4545
XmnI GAAnn'nnTTC
Cuts at: 4001 4001
Size: 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BstZ17I DraIII EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI
PmlI PpuMI PspOMI PspXI RsrII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI