Restriction map for
pFN21AA0104
(Circular) (Six-base) MAPSORT of: pFN21AA0104.seq Check: 9831 from: 1 to: 5581
pFN21AA0104 5581 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3391 278
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2924 1937
Size: 987 4594
Acc65I G'GTAC_C
Cuts at: 2902 2902
Size: 5581
AclI AA'CG_TT
Cuts at: 1563 3708 4081 1563
Size: 2145 373 3063
Fragments arranged by size:
3063 2145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3644 5028 5296 1632
Size: 2012 1384 268 1917
Fragments arranged by size:
2012 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5384
AflIII A'CryG_T
Cuts at: 1203 4480 5457 1203
Size: 3277 977 1327
Fragments arranged by size:
3277 1327 977
AgeI A'CCGG_T
Cuts at: 2182 4412 2182
Size: 2230 3351
AhdI GACnn_n'nnGTC
Cuts at: 2480 4310 2480
Size: 1830 3751
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2052 1184
Size: 868 4713
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2210 2242 2210
Size: 32 5549
AlwNI CAG_nnn'CTG
Cuts at: 2404 3324 4896 2404
Size: 920 1572 3089
Fragments arranged by size:
3089 1572 920
ApaLI G'TGCA_C
Cuts at: 3638 4794 3638
Size: 1156 4425
ApoI r'AATT_y
Cuts at: 1493 2240 2276 2713 2801 2890 3116 3268 5377
Size: 747 36 437 88 89 226 152 2109
Cuts at: 5377 1493
Size: 1697
Fragments arranged by size:
2109 1697 747 437 226 152 89 88
36
AseI AT'TA_AT
Cuts at: 160 1032 4135 160
Size: 872 3103 1606
Fragments arranged by size:
3103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
AvaI C'yCGr_G
Cuts at: 1870 1943 2511 2906 1870
Size: 73 568 395 4545
Fragments arranged by size:
4545 568 395 73
AvrII C'CTAG_G
Cuts at: 2047 2047
Size: 5581
BamHI G'GATC_C
Cuts at: 2911 3278 2911
Size: 367 5214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2902 4357 618
Size: 325 217 627 1115 1455 1842
Fragments arranged by size:
1842 1455 1115 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2157 2900 1391
Size: 766 743 4072
Fragments arranged by size:
4072 766 743
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2141 2175 3772 3806 1903
Size: 34 204 34 1597 34 3678
Fragments arranged by size:
3678 1597 204 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3473 4683 3473
Size: 1210 4371
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2128 4192
Size: 107 122 71 631 605 337 119 2064
Cuts at: 4192 136
Size: 1525
Fragments arranged by size:
2064 1525 631 605 337 122 119 107
71
BglII A'GATC_T
Cuts at: 5576 5576
Size: 5581
BlpI GC'TnA_GC
Cuts at: 2998 2998
Size: 5581
Bme1580I G_kGCm'C
Cuts at: 1790 2031 3642 4798 1790
Size: 241 1611 1156 2573
Fragments arranged by size:
2573 1611 1156 241
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2041 2151 3312
Size: 416 53 83 399 24 624 110 1161
Cuts at: 3312 4270 442
Size: 958 1753
Fragments arranged by size:
1753 1161 958 624 416 399 110 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4241 1239
Size: 3002 2579
Bpu10I CC'TnA_GC
Cuts at: 2682 2682
Size: 5581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2251 3706 4571 4869 2251
Size: 1455 865 298 2963
Fragments arranged by size:
2963 1455 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4244 882
Size: 617 2745 2219
Fragments arranged by size:
2745 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3372 493
Size: 711 2168 2702
Fragments arranged by size:
2702 2168 711
BsaBI GATnn'nnATC
Cuts at: 5575 5575
Size: 5581
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3388 3770 4405 275
Size: 53 83 186 936 1855 382 635 1451
Fragments arranged by size:
1855 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2182 2215 4014 4412 4686 4833 5244 2182
Size: 33 1799 398 274 147 411 2519
Fragments arranged by size:
2519 1799 411 398 274 147 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5356
Fragments arranged by size:
5356 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5581
BseYI C'CCAG_C
Cuts at: 2700 4784 5280 2700
Size: 2084 496 3001
Fragments arranged by size:
3001 2084 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5008
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3031 3792 4820 1540
Size: 275 186 1030 761 1028 2301
Fragments arranged by size:
2301 1030 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2141 3642 3727 4798 2141
Size: 1501 85 1071 2924
Fragments arranged by size:
2924 1501 1071 85
BsmI GAATG_Cn'
Cuts at: 2415 3092 3185 2415
Size: 677 93 4811
Fragments arranged by size:
4811 677 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2141 2157 2900 3642 3727 4798
Size: 399 241 110 16 743 742 85 1071
Cuts at: 4798 1391
Size: 2174
Fragments arranged by size:
2174 1071 743 742 399 241 110 85
16
BspEI T'CCGG_A
Cuts at: 5244 5244
Size: 5581
BspHI T'CATG_A
Cuts at: 3470 5200 3470
Size: 1730 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2214 2936 844
Size: 1370 722 3489
Fragments arranged by size:
3489 1370 722
BsrBI CCG'CTC
Cuts at: 1394 1823 3468 1394
Size: 429 1645 3507
Fragments arranged by size:
3507 1645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2016 2046 4076 4250 65
Size: 1183 768 30 2030 174 1396
Fragments arranged by size:
2030 1396 1183 768 174 30
BsrFI r'CCGG_y
Cuts at: 1700 1810 2089 2182 4225 4412 1700
Size: 110 279 93 2043 187 2869
Fragments arranged by size:
2869 2043 279 187 110 93
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5581
BssSI C'ACGA_G
Cuts at: 3641 4653 3641
Size: 1012 4569
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5581
BstBI TT'CG_AA
Cuts at: 4385 4385
Size: 5581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5581
BstYI r'GATC_y
Cuts at: 1972 2266 2911 3278 3663 3680 5132 5218 5230
Size: 294 645 367 385 17 1452 86 12
Cuts at: 5230 5576 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 645 385 367 346 294 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2456 2456
Size: 5581
BtgI C'CryG_G
Cuts at: 513 1065 2004 2208 2792 513
Size: 552 939 204 584 3302
Fragments arranged by size:
3302 939 584 552 204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4842
Fragments arranged by size:
4842 683 56
BtsI GCAGTG_nn'
Cuts at: 3097 3891 3911 3097
Size: 794 20 4767
Fragments arranged by size:
4767 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5546
DraI TTT'AAA
Cuts at: 2885 2885
Size: 5581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4588 809
Size: 507 3272 1802
Fragments arranged by size:
3272 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3028 3917 5546
Size: 54 1425 50 275 18 1198 889 1629
Cuts at: 5546 8
Size: 43
Fragments arranged by size:
1629 1425 1198 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3028 1537
Size: 275 1216 4090
Fragments arranged by size:
4090 1216 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2425 3511 1353
Size: 1072 1086 3423
Fragments arranged by size:
3423 1086 1072
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4168 4542 4688 387
Size: 1064 138 2579 374 146 1280
Fragments arranged by size:
2579 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3644 4241 5028 5296 1239
Size: 393 2012 597 787 268 1524
Fragments arranged by size:
2012 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2120 2120
Size: 5581
EcoRI G'AATT_C
Cuts at: 2240 2890 3268 2240
Size: 650 378 4553
Fragments arranged by size:
4553 650 378
FspI TGC'GCA
Cuts at: 4087 4087
Size: 5581
HaeII r_GCGC'y
Cuts at: 1678 4728 1678
Size: 3050 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2466 2498 4222 4254 4296
Size: 32 135 32 894 32 1724 32 42
Cuts at: 4296 4328 1373
Size: 32 2626
Fragments arranged by size:
2626 1724 894 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2925 3171 669
Size: 988 281 987 246 3079
Fragments arranged by size:
3079 988 987 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5581
HpaI GTT'AAC
Cuts at: 3171 3171
Size: 5581
KpnI G_GTAC'C
Cuts at: 2906 2906
Size: 5581
MfeI C'AATT_G
Cuts at: 3180 3180
Size: 5581
MluI A'CGCG_T
Cuts at: 5457 5457
Size: 5581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2191 2323 4695 4879 1305
Size: 886 132 2372 184 2007
Fragments arranged by size:
2372 2007 886 184 132
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3759
Fragments arranged by size:
3759 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3539 3898 4057 518
Size: 648 2373 359 159 2042
Fragments arranged by size:
2373 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2369 2997 3674 4822 5067 1606
Size: 418 345 628 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 628 418 345 245
NaeI GCC'GGC
Cuts at: 2091 2091
Size: 5581
NcoI C'CATG_G
Cuts at: 513 1065 2004 2792 513
Size: 552 939 788 3302
Fragments arranged by size:
3302 939 788 552
NdeI CA'TA_TG
Cuts at: 387 2377 387
Size: 1990 3591
NgoMIV G'CCGG_C
Cuts at: 2089 2089
Size: 5581
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5581
NotI GC'GGCC_GC
Cuts at: 3028 3028
Size: 5581
NspI r_CATG'y
Cuts at: 1132 1229 2939 4484 1132
Size: 97 1710 1545 2229
Fragments arranged by size:
2229 1710 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5581
PciI A'CATG_T
Cuts at: 4480 4480
Size: 5581
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2404 1108
Size: 1296 4285
PfoI T'CCnGG_A
Cuts at: 2517 2517
Size: 5581
PmeI GTTT'AAAC
Cuts at: 2885 2885
Size: 5581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2286 3650 3682 5163 5195 2254
Size: 32 1364 32 1481 32 2640
Fragments arranged by size:
2640 1481 1364 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5581
PsiI TTA'TAA
Cuts at: 2505 3151 2505
Size: 646 4935
PstI C_TGCA'G
Cuts at: 830 2108 2933 830
Size: 1278 825 3478
Fragments arranged by size:
3478 1278 825
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5581
PvuII CAG'CTG
Cuts at: 2369 2369
Size: 5581
SalI G'TCGA_C
Cuts at: 1936 2923 1936
Size: 987 4594
SbfI CC_TGCA'GG
Cuts at: 2108 2933 2108
Size: 825 4756
ScaI AGT'ACT
Cuts at: 2319 3829 5541 2319
Size: 1510 1712 2359
Fragments arranged by size:
2359 1712 1510
SfcI C'TryA_G
Cuts at: 826 1045 2104 2364 2929 4064 4745 4936 826
Size: 219 1059 260 565 1135 681 191 1471
Fragments arranged by size:
1471 1135 1059 681 565 260 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5581
SmaI CCC'GGG
Cuts at: 1872 2908 1872
Size: 1036 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2230 3685 4586 4848 820
Size: 197 926 287 1455 901 262 1553
Fragments arranged by size:
1553 1455 926 901 287 262 197
SnaBI TAC'GTA
Cuts at: 493 3372 493
Size: 2879 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5581
SphI G_CATG'C
Cuts at: 1132 2939 1132
Size: 1807 3774
SspI AAT'ATT
Cuts at: 5 52 2722 3505 5
Size: 47 2670 783 2081
Fragments arranged by size:
2670 2081 783 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5581
StyI C'CwwG_G
Cuts at: 513 1065 2004 2047 2792 3020 513
Size: 552 939 43 745 228 3074
Fragments arranged by size:
3074 939 745 552 228 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3608 3625 3778 3963 4408 325
Size: 1255 2028 17 153 185 445 1498
Fragments arranged by size:
2028 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2317 2626 3827
Size: 274 80 33 51 1443 340 309 1201
Cuts at: 3827 5539 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1201 340 309 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5541 5573 5541
Size: 32 5549
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5581
XbaI T'CTAG_A
Cuts at: 2917 2917
Size: 5581
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5581
XmaI C'CCGG_G
Cuts at: 1870 2906 1870
Size: 1036 4545
XmnI GAAnn'nnTTC
Cuts at: 2244 3710 2244
Size: 1466 4115
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3389 276
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI