Restriction map for
pFN21AA0103
(Circular) (Six-base) MAPSORT of: pFN21AA0103.seq Check: 4574 from: 1 to: 5596
pFN21AA0103 5596 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2603 2939 1937
Size: 666 336 4594
Fragments arranged by size:
4594 666 336
Acc65I G'GTAC_C
Cuts at: 2917 2917
Size: 5596
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2613 3659 5043 5311 1632
Size: 981 1046 1384 268 1917
Fragments arranged by size:
1917 1384 1046 981 268
AflII C'TTAA_G
Cuts at: 820 1017 2849 820
Size: 197 1832 3567
Fragments arranged by size:
3567 1832 197
AflIII A'CryG_T
Cuts at: 1203 2865 4495 5472 1203
Size: 1662 1630 977 1327
Fragments arranged by size:
1662 1630 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2502 1184
Size: 1318 4278
AlwNI CAG_nnn'CTG
Cuts at: 3339 4911 3339
Size: 1572 4024
ApaLI G'TGCA_C
Cuts at: 3653 4809 3653
Size: 1156 4440
ApoI r'AATT_y
Cuts at: 1493 2905 3131 3283 5392 1493
Size: 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2123 2127 4150 160
Size: 872 1091 4 2023 1606
Fragments arranged by size:
2023 1606 1091 872 4
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2921 1870
Size: 73 978 4545
Fragments arranged by size:
4545 978 73
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2917 4372 618
Size: 325 217 627 1130 1455 1842
Fragments arranged by size:
1842 1455 1130 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2915 1391
Size: 1524 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2222 2251 2868 928
Size: 971 323 29 617 3656
Fragments arranged by size:
3656 971 617 323 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4207 136
Size: 107 122 71 631 605 2535 1525
Fragments arranged by size:
2535 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 3657 4813 1790
Size: 1867 1156 2573
Fragments arranged by size:
2573 1867 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2884 3327 4285
Size: 416 53 83 399 24 1467 443 958
Cuts at: 4285 442
Size: 1753
Fragments arranged by size:
1753 1467 958 443 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4256 1239
Size: 3017 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2068 3721 4586 4884 2068
Size: 1653 865 298 2780
Fragments arranged by size:
2780 1653 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2020 2457 4259 882
Size: 617 521 437 1802 2219
Fragments arranged by size:
2219 1802 617 521 437
BsaAI yAC'GTr
Cuts at: 493 1204 3387 493
Size: 711 2183 2702
Fragments arranged by size:
2702 2183 711
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3403 3785 4420 275
Size: 53 83 186 936 1870 382 635 1451
Fragments arranged by size:
1870 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4029 4427 4701 4848 5259 4029
Size: 398 274 147 411 4366
Fragments arranged by size:
4366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5371
Fragments arranged by size:
5371 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5596
BseYI C'CCAG_C
Cuts at: 2146 4799 5295 2146
Size: 2653 496 2447
Fragments arranged by size:
2653 2447 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5023
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3807 4835 1540
Size: 275 186 1045 761 1028 2301
Fragments arranged by size:
2301 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3657 3742 4813 3657
Size: 85 1071 4440
Fragments arranged by size:
4440 1071 85
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2915 3657 3742 4813 1391
Size: 399 1125 742 85 1071 2174
Fragments arranged by size:
2174 1125 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2807 2951 844
Size: 1963 144 3489
Fragments arranged by size:
3489 1963 144
BsrBI CCG'CTC
Cuts at: 1394 1823 3483 1394
Size: 429 1660 3507
Fragments arranged by size:
3507 1660 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2876 4091 4265 65
Size: 1183 1628 1215 174 1396
Fragments arranged by size:
1628 1396 1215 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5596
BstBI TT'CG_AA
Cuts at: 2395 4400 2395
Size: 2005 3591
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2353 2926 3293 3678 3695 5147 5233 5245
Size: 381 573 367 385 17 1452 86 12
Cuts at: 5245 5591 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 573 385 381 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2604 2604
Size: 5596
BtgI C'CryG_G
Cuts at: 513 1065 2004 2301 513
Size: 552 939 297 3808
Fragments arranged by size:
3808 939 552 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 2364 3112 3906 3926 2364
Size: 748 794 20 4034
Fragments arranged by size:
4034 794 748 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3043 3932 5561
Size: 54 1425 50 275 18 1213 889 1629
Cuts at: 5561 8
Size: 43
Fragments arranged by size:
1629 1425 1213 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3526 1353
Size: 2173 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4183 4557 4703 387
Size: 1064 138 2594 374 146 1280
Fragments arranged by size:
2594 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2613 3659 4256 5043 5311 1239
Size: 393 981 1046 597 787 268 1524
Fragments arranged by size:
1524 1046 981 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2633 2633
Size: 5596
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 4743 1678
Size: 3065 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2542 2574 4237 4269 4311
Size: 32 135 32 970 32 1663 32 42
Cuts at: 4311 4343 1373
Size: 32 2626
Fragments arranged by size:
2626 1663 970 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 3186 669
Size: 988 281 1002 246 3079
Fragments arranged by size:
3079 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2921 2921
Size: 5596
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2092 2381 2440 2850 4710 4894 1305
Size: 787 289 59 410 1860 184 2007
Fragments arranged by size:
2007 1860 787 410 289 184 59
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3774
Fragments arranged by size:
3774 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2514 3554 3913 4072 518
Size: 648 1348 1040 359 159 2042
Fragments arranged by size:
2042 1348 1040 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3012 3689 4837 5082 1606
Size: 1406 677 1148 245 2120
Fragments arranged by size:
2120 1406 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2301 513
Size: 552 939 297 3808
Fragments arranged by size:
3808 939 552 297
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2869 2954 4499 1132
Size: 97 1640 85 1545 2229
Fragments arranged by size:
2229 1640 1545 97 85
PacI TTA_AT'TAA
Cuts at: 2127 2127
Size: 5596
PasI CC'CwG_GG
Cuts at: 1348 2404 1348
Size: 1056 4540
PciI A'CATG_T
Cuts at: 2865 4495 2865
Size: 1630 3966
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2202 1613
Size: 589 5007
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2948 830
Size: 2118 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2944 4079 4760 4951 826
Size: 219 1899 1135 681 191 1471
Fragments arranged by size:
1899 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2923 1872
Size: 1051 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2083 2849 3700 4601 4863 820
Size: 197 926 140 766 851 901 262 1553
Fragments arranged by size:
1553 926 901 851 766 262 197 140
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 2843 3520 5
Size: 47 2791 677 2081
Fragments arranged by size:
2791 2081 677 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 2004 2301 2608 3035 513
Size: 552 939 297 307 427 3074
Fragments arranged by size:
3074 939 552 427 307 297
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2903 3623 3640 3793 3978 4423 325
Size: 1255 1323 720 17 153 185 445 1498
Fragments arranged by size:
1498 1323 1255 720 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5556 5588 5556
Size: 32 5564
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2921 1870
Size: 1051 4545
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PacI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI