Restriction map for
pFN21AA0100
(Circular) (Six-base) MAPSORT of: pFN21AA0100.seq Check: 783 from: 1 to: 11410
pFN21AA0100 11410 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2597 3076 3183 5622 6255 2597
Size: 479 107 2439 633 7752
Fragments arranged by size:
7752 2439 633 479 107
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3830 9220 278
Size: 53 83 186 936 2294 5390 2468
Fragments arranged by size:
5390 2468 2294 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5134 8753 1937
Size: 3197 3619 4594
Fragments arranged by size:
4594 3619 3197
Acc65I G'GTAC_C
Cuts at: 5097 8731 5097
Size: 3634 7776
AclI AA'CG_TT
Cuts at: 1563 9537 9910 1563
Size: 7974 373 3063
Fragments arranged by size:
7974 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2353 2933 3578 3765 4113 4568 6219 6488
Size: 721 580 645 187 348 455 1651 269
Cuts at: 6488 6550 7218 7562 7957 8573 9473 10857 11125
Size: 62 668 344 395 616 900 1384 268
Cuts at: 11125 1632
Size: 1917
Fragments arranged by size:
1917 1651 1384 900 721 668 645 616
580 455 395 348 344 269 268 187
62
AfeI AGC'GCT
Cuts at: 2341 3861 2341
Size: 1520 9890
AflII C'TTAA_G
Cuts at: 820 1017 2174 820
Size: 197 1157 10056
Fragments arranged by size:
10056 1157 197
AflIII A'CryG_T
Cuts at: 1203 4029 6606 10309 11286 1203
Size: 2826 2577 3703 977 1327
Fragments arranged by size:
3703 2826 2577 1327 977
AgeI A'CCGG_T
Cuts at: 10241 10241
Size: 11410
AhdI GACnn_n'nnGTC
Cuts at: 10139 10139
Size: 11410
AleI CACnn'nnGTG
Cuts at: 1166 2864 5288 5679 1166
Size: 1698 2424 391 6897
Fragments arranged by size:
6897 2424 1698 391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2673 3023 6128 6924 7463 8208 1184
Size: 1489 350 3105 796 539 745 4386
Fragments arranged by size:
4386 3105 1489 796 745 539 350
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7620 7652 7620
Size: 32 11378
AlwNI CAG_nnn'CTG
Cuts at: 2827 2929 3535 4735 5650 7015 8570 9153 10725
Size: 102 606 1200 915 1365 1555 583 1572
Cuts at: 10725 2827
Size: 3512
Fragments arranged by size:
3512 1572 1555 1365 1200 915 606 583
102
ApaI G_GGCC'C
Cuts at: 2738 5300 5698 8222 2738
Size: 2562 398 2524 5926
Fragments arranged by size:
5926 2562 2524 398
ApaLI G'TGCA_C
Cuts at: 9467 10623 9467
Size: 1156 10254
ApoI r'AATT_y
Cuts at: 1493 3155 5246 5826 7287 8719 8945 9097 11206
Size: 1662 2091 580 1461 1432 226 152 2109
Cuts at: 11206 1493
Size: 1697
Fragments arranged by size:
2109 2091 1697 1662 1461 1432 580 226
152
AseI AT'TA_AT
Cuts at: 160 1032 9964 160
Size: 872 8932 1606
Fragments arranged by size:
8932 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11410
AvaI C'yCGr_G
Cuts at: 1870 1943 6072 8735 1870
Size: 73 4129 2663 4545
Fragments arranged by size:
4545 4129 2663 73
BamHI G'GATC_C
Cuts at: 2157 5308 5470 6325 8740 9107 2157
Size: 3151 162 855 2415 367 4460
Fragments arranged by size:
4460 3151 2415 855 367 162
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2895 3147 3451 5097 5144
Size: 325 217 627 1108 252 304 1646 47
Cuts at: 5144 5716 6117 7758 8035 8731 10186 618
Size: 572 401 1641 277 696 1455 1842
Fragments arranged by size:
1842 1646 1641 1455 1108 696 627 572
401 325 304 277 252 217 47
BanII G_rGCy'C
Cuts at: 1391 2738 4019 4806 5300 5698 6309 7937 8222
Size: 1347 1281 787 494 398 611 1628 285
Cuts at: 8222 8729 1391
Size: 507 4072
Fragments arranged by size:
4072 1628 1347 1281 787 611 507 494
398 285
BbeI G_GCGC'C
Cuts at: 3455 6121 7762 3455
Size: 2666 1641 7103
Fragments arranged by size:
7103 2666 1641
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3747 5396 6058 928
Size: 971 1848 1649 662 6280
Fragments arranged by size:
6280 1848 1649 971 662
BbvCI CC'TCA_GC
Cuts at: 4239 5359 6297 6535 7080 4239
Size: 1120 938 238 545 8569
Fragments arranged by size:
8569 1120 938 545 238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6269 6303 9601 9635 1903
Size: 34 50 34 4248 34 3298 34 3678
Fragments arranged by size:
4248 3678 3298 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2443 7249 9302 10512 2443
Size: 4806 2053 1210 3341
Fragments arranged by size:
4806 3341 2053 1210
BclI T'GATC_A
Cuts at: 1548 5671 6211 1548
Size: 4123 540 6747
Fragments arranged by size:
6747 4123 540
BfrBI ATG'CAT
Cuts at: 3472 3472
Size: 11410
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 10021 136
Size: 107 122 71 631 605 8349 1525
Fragments arranged by size:
8349 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2388 5020 5184 7225 7410 11405 2388
Size: 2632 164 2041 185 3995 2393
Fragments arranged by size:
3995 2632 2393 2041 185 164
BlpI GC'TnA_GC
Cuts at: 2051 3929 4906 6251 8827 2051
Size: 1878 977 1345 2576 4634
Fragments arranged by size:
4634 2576 1878 1345 977
Bme1580I G_kGCm'C
Cuts at: 1790 2738 3622 4162 4891 5147 5300 5698 5721
Size: 948 884 540 729 256 153 398 23
Cuts at: 5721 8222 9471 10627 1790
Size: 2501 1249 1156 2573
Fragments arranged by size:
2573 2501 1249 1156 948 884 729 540
398 256 153 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11410
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3547 3917 5509
Size: 416 53 83 399 24 2130 370 1592
Cuts at: 5509 8225 8425 9141 10099 442
Size: 2716 200 716 958 1753
Fragments arranged by size:
2716 2130 1753 1592 958 716 416 399
370 200 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3182 7961 1056
Size: 2126 4779 4505
Fragments arranged by size:
4779 4505 2126
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4651 6325 4651
Size: 1674 9736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2600 2804 3998 4223 7517 7883 8468 10070
Size: 1361 204 1194 225 3294 366 585 1602
Cuts at: 10070 1239
Size: 2579
Fragments arranged by size:
3294 2579 1602 1361 1194 585 366 225
204
Bpu10I CC'TnA_GC
Cuts at: 2237 4164 4239 4386 4956 5359 6297 6535 7080
Size: 1927 75 147 570 403 938 238 545
Cuts at: 7080 7359 7746 8546 2237
Size: 279 387 800 5101
Fragments arranged by size:
5101 1927 938 800 570 545 403 387
279 238 147 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2426 3056 5033 6081 7226 7427 9535 10400 10698
Size: 630 1977 1048 1145 201 2108 865 298
Cuts at: 10698 2426
Size: 3138
Fragments arranged by size:
3138 2108 1977 1145 1048 865 630 298
201
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4065 10073 882
Size: 617 2566 6008 2219
Fragments arranged by size:
6008 2566 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 9201 493
Size: 711 7997 2702
Fragments arranged by size:
7997 2702 711
BsaBI GATnn'nnATC
Cuts at: 2946 6324 11404 2946
Size: 3378 5080 2952
Fragments arranged by size:
5080 3378 2952
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3393 3452 3827 5179
Size: 53 83 186 936 1860 59 375 1352
Cuts at: 5179 6118 7759 9217 9599 10234 275
Size: 939 1641 1458 382 635 1451
Fragments arranged by size:
1860 1641 1458 1451 1352 939 936 635
382 375 186 83 59 53
BsaWI w'CCGG_w
Cuts at: 2085 4410 4440 9843 10241 10515 10662 11073 2085
Size: 2325 30 5403 398 274 147 411 2422
Fragments arranged by size:
5403 2422 2325 411 398 274 147 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3971 4001 1178
Size: 30 165 30 2568 30 8587
Fragments arranged by size:
8587 2568 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4448 4814 6314 6317 6992 7442 7883 8636
Size: 3262 366 1500 3 675 450 441 753
Cuts at: 8636 8642 8645 1186
Size: 6 3 3951
Fragments arranged by size:
3951 3262 1500 753 675 450 441 366
6 3 3
BseYI C'CCAG_C
Cuts at: 2342 5304 5369 5817 5933 5991 6024 6560 7095
Size: 2962 65 448 116 58 33 536 535
Cuts at: 7095 7300 7783 10613 11109 2342
Size: 205 483 2830 496 2643
Fragments arranged by size:
2962 2830 2643 536 535 496 483 448
205 116 65 58 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3104 3155 3840 4014 4394 5435 6070
Size: 573 1368 51 685 174 380 1041 635
Cuts at: 6070 6335 7028 1163
Size: 265 693 5545
Fragments arranged by size:
5545 1368 1041 693 685 635 573 380
265 174 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8860 9621 10649 1540
Size: 275 186 6859 761 1028 2301
Fragments arranged by size:
6859 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3002 3930 4214 4632 4806 6309 6984 7937 8295
Size: 928 284 418 174 1503 675 953 358
Cuts at: 8295 9471 9556 10627 3002
Size: 1176 85 1071 3785
Fragments arranged by size:
3785 1503 1176 1071 953 928 675 418
358 284 174 85
BsmI GAATG_Cn'
Cuts at: 5049 6211 8921 9014 5049
Size: 1162 2710 93 7445
Fragments arranged by size:
7445 2710 1162 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3818 4865 3818
Size: 1047 10363
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2738 3002 3622 3930 4019 4162 4214
Size: 399 948 264 620 308 89 143 52
Cuts at: 4214 4632 4806 4891 5147 5300 5698 5721 6309
Size: 418 174 85 256 153 398 23 588
Cuts at: 6309 6984 7937 8222 8295 8729 9471 9556 10627
Size: 675 953 285 73 434 742 85 1071
Cuts at: 10627 1391
Size: 2174
Fragments arranged by size:
2174 1071 953 948 742 675 620 588
434 418 399 398 308 285 264 256
174 153 143 89 85 85 73 52
23
BspEI T'CCGG_A
Cuts at: 4440 11073 4440
Size: 6633 4777
BspHI T'CATG_A
Cuts at: 4675 5734 7518 9299 11029 4675
Size: 1059 1784 1781 1730 5056
Fragments arranged by size:
5056 1784 1781 1730 1059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2597 3076 3183 5457 5622 5649 5989 6255
Size: 1753 479 107 2274 165 27 340 266
Cuts at: 6255 7347 7617 8765 844
Size: 1092 270 1148 3489
Fragments arranged by size:
3489 2274 1753 1148 1092 479 340 270
266 165 107 27
BsrBI CCG'CTC
Cuts at: 1394 1823 3972 9297 1394
Size: 429 2149 5325 3507
Fragments arranged by size:
5325 3507 2149 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5062 6396 6690 9905 10079 65
Size: 1183 3814 1334 294 3215 174 1396
Fragments arranged by size:
3814 3215 1396 1334 1183 294 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2070 3144 4284 6984 10054 10241 1700
Size: 110 260 1074 1140 2700 3070 187 2869
Fragments arranged by size:
3070 2869 2700 1140 1074 260 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 11410
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11410
BssSI C'ACGA_G
Cuts at: 4569 6315 6546 9470 10482 4569
Size: 1746 231 2924 1012 5497
Fragments arranged by size:
5497 2924 1746 1012 231
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3057 794
Size: 2263 9147
BstBI TT'CG_AA
Cuts at: 2962 10214 2962
Size: 7252 4158
BstEII G'GTnAC_C
Cuts at: 1184 3088 6169 7048 8420 1184
Size: 1904 3081 879 1372 4174
Fragments arranged by size:
4174 3081 1904 1372 879
BstXI CCAn_nnnn'nTGG
Cuts at: 2342 5785 6686 7321 2342
Size: 3443 901 635 6431
Fragments arranged by size:
6431 3443 901 635
BstYI r'GATC_y
Cuts at: 1972 2157 2388 5020 5109 5184 5308 5470 5788
Size: 185 231 2632 89 75 124 162 318
Cuts at: 5788 6325 7225 7410 8740 9107 9492 9509 10961
Size: 537 900 185 1330 367 385 17 1452
Cuts at: 10961 11047 11059 11405 1972
Size: 86 12 346 1977
Fragments arranged by size:
2632 1977 1452 1330 900 537 385 367
346 318 231 185 185 162 124 89
86 75 17 12
Bsu36I CC'TnA_GG
Cuts at: 3365 5010 5712 3365
Size: 1645 702 9063
Fragments arranged by size:
9063 1645 702
BtgI C'CryG_G
Cuts at: 513 1065 4948 5093 5204 5413 5656 8487 513
Size: 552 3883 145 111 209 243 2831 3436
Fragments arranged by size:
3883 3436 2831 552 243 209 145 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10671
Fragments arranged by size:
10671 683 56
BtsI GCAGTG_nn'
Cuts at: 2655 3436 3823 4889 5226 5651 6830 7041 8926
Size: 781 387 1066 337 425 1179 211 1885
Cuts at: 8926 9720 9740 2655
Size: 794 20 4325
Fragments arranged by size:
4325 1885 1179 1066 794 781 425 387
337 211 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11410
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11375
DraI TTT'AAA
Cuts at: 8714 8714
Size: 11410
DraIII CAC_nnn'GTG
Cuts at: 2100 2260 6548 8299 8381 2100
Size: 160 4288 1751 82 5129
Fragments arranged by size:
5129 4288 1751 160 82
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3734 10417 809
Size: 507 2418 6683 1802
Fragments arranged by size:
6683 2418 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 4053 4749
Size: 54 1425 50 275 18 261 1962 696
Cuts at: 4749 5329 5460 6943 8098 8857 9746 11375 8
Size: 580 131 1483 1155 759 889 1629 43
Fragments arranged by size:
1962 1629 1483 1425 1155 889 759 696
580 275 261 131 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8857 1537
Size: 275 7045 4090
Fragments arranged by size:
7045 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2714 3694 4389 5921 6195 6621 6651 7976
Size: 1361 980 695 1532 274 426 30 1325
Cuts at: 7976 8127 8619 9340 1353
Size: 151 492 721 3423
Fragments arranged by size:
3423 1532 1361 1325 980 721 695 492
426 274 151 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2138 7955 9997 10371 10517 387
Size: 1064 138 549 5817 2042 374 146 1280
Fragments arranged by size:
5817 2042 1280 1064 549 374 146 138
EcoICRI GAG'CTC
Cuts at: 4804 6307 7935 4804
Size: 1503 1628 8279
Fragments arranged by size:
8279 1628 1503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2353 2600 2804 2933 3578 3765 3998
Size: 393 721 247 204 129 645 187 233
Cuts at: 3998 4113 4223 4568 6219 6488 6550 7218 7517
Size: 115 110 345 1651 269 62 668 299
Cuts at: 7517 7562 7883 7957 8468 8573 9473 10070 10857
Size: 45 321 74 511 105 900 597 787
Cuts at: 10857 11125 1239
Size: 268 1524
Fragments arranged by size:
1651 1524 900 787 721 668 645 597
511 393 345 321 299 269 268 247
233 204 187 129 115 110 105 74
62 45
EcoNI CCTnn'n_nnAGG
Cuts at: 5711 6099 6164 7766 5711
Size: 388 65 1602 9355
Fragments arranged by size:
9355 1602 388 65
EcoO109I rG'GnC_Cy
Cuts at: 2704 2734 3904 5296 5695 6463 8219 2704
Size: 30 1170 1392 399 768 1756 5895
Fragments arranged by size:
5895 1756 1392 1170 768 399 30
EcoRI G'AATT_C
Cuts at: 3155 5826 8719 9097 3155
Size: 2671 2893 378 5468
Fragments arranged by size:
5468 2893 2671 378
EcoRV GAT'ATC
Cuts at: 5566 5566
Size: 11410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3745 5575 8710 3745
Size: 1830 3135 6445
Fragments arranged by size:
6445 3135 1830
FspI TGC'GCA
Cuts at: 9916 9916
Size: 11410
HaeII r_GCGC'y
Cuts at: 1678 2058 2343 3455 3863 6121 7762 10557 1678
Size: 380 285 1112 408 2258 1641 2795 2531
Fragments arranged by size:
2795 2531 2258 1641 1112 408 380 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2893 2925 4259 4291 5249
Size: 32 135 32 1321 32 1334 32 958
Cuts at: 5249 5281 6572 6604 8126 8158 10051 10083 10125
Size: 32 1291 32 1522 32 1893 32 42
Cuts at: 10125 10157 1373
Size: 32 2626
Fragments arranged by size:
2626 1893 1522 1334 1321 1291 958 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3289 5135 6553 6748 7333 8754
Size: 988 281 1351 1846 1418 195 585 1421
Cuts at: 8754 9000 669
Size: 246 3079
Fragments arranged by size:
3079 1846 1421 1418 1351 988 585 281
246 195
HindIII A'AGCT_T
Cuts at: 748 2965 7574 748
Size: 2217 4609 4584
Fragments arranged by size:
4609 4584 2217
HpaI GTT'AAC
Cuts at: 6553 9000 6553
Size: 2447 8963
KasI G'GCGC_C
Cuts at: 3451 6117 7758 3451
Size: 2666 1641 7103
Fragments arranged by size:
7103 2666 1641
KpnI G_GTAC'C
Cuts at: 5101 8735 5101
Size: 3634 7776
MfeI C'AATT_G
Cuts at: 9009 9009
Size: 11410
MluI A'CGCG_T
Cuts at: 11286 11286
Size: 11410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7687 10524 10708 1305
Size: 6382 2837 184 2007
Fragments arranged by size:
6382 2837 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 4055 5331 5462 10
Size: 54 1425 343 261 1962 1276 131 5958
Fragments arranged by size:
5958 1962 1425 1276 343 261 131 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2418 2864 2940 3715 4877 5288 5679
Size: 648 1252 446 76 775 1162 411 391
Cuts at: 5679 5783 6110 8470 9368 9727 9886 518
Size: 104 327 2360 898 359 159 2042
Fragments arranged by size:
2360 2042 1252 1162 898 775 648 446
411 391 359 327 159 104 76
MspA1I CmG'CkG
Cuts at: 1606 2110 2578 2827 2953 3388 3448 3674 4378
Size: 504 468 249 126 435 60 226 704
Cuts at: 4378 5363 5458 5995 6085 6296 6349 6434 6803
Size: 985 95 537 90 211 53 85 369
Cuts at: 6803 7079 7108 7495 7615 7672 7783 8053 8188
Size: 276 29 387 120 57 111 270 135
Cuts at: 8188 8826 9503 10651 10896 1606
Size: 638 677 1148 245 2120
Fragments arranged by size:
2120 1148 985 704 677 638 537 504
468 435 387 369 276 270 249 245
226 211 135 126 120 111 95 90
85 60 57 53 29
NaeI GCC'GGC
Cuts at: 4286 4286
Size: 11410
NarI GG'CG_CC
Cuts at: 3452 6118 7759 3452
Size: 2666 1641 7103
Fragments arranged by size:
7103 2666 1641
NcoI C'CATG_G
Cuts at: 513 1065 4948 5413 5656 8487 513
Size: 552 3883 465 243 2831 3436
Fragments arranged by size:
3883 3436 2831 552 465 243
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11410
NgoMIV G'CCGG_C
Cuts at: 4284 4284
Size: 11410
NheI G'CTAG_C
Cuts at: 1052 3178 7957 1052
Size: 2126 4779 4505
Fragments arranged by size:
4779 4505 2126
NotI GC'GGCC_GC
Cuts at: 8857 8857
Size: 11410
NsiI A_TGCA'T
Cuts at: 3474 3474
Size: 11410
NspI r_CATG'y
Cuts at: 1132 1229 3476 4033 4049 4964 5236 6610 6901
Size: 97 2247 557 16 915 272 1374 291
Cuts at: 6901 7478 8614 8768 10313 1132
Size: 577 1136 154 1545 2229
Fragments arranged by size:
2247 2229 1545 1374 1136 915 577 557
291 272 154 97 16
PasI CC'CwG_GG
Cuts at: 1348 8222 1348
Size: 6874 4536
PciI A'CATG_T
Cuts at: 4029 6606 10309 4029
Size: 2577 3703 5130
Fragments arranged by size:
5130 3703 2577
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2100 3250 3535 3662 5338 7065 7282 1108
Size: 992 1150 285 127 1676 1727 217 5236
Fragments arranged by size:
5236 1727 1676 1150 992 285 217 127
PfoI T'CCnGG_A
Cuts at: 6374 6875 7320 6374
Size: 501 445 10464
Fragments arranged by size:
10464 501 445
PmeI GTTT'AAAC
Cuts at: 8714 8714
Size: 11410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 9479 9511 10992 11024 4232
Size: 32 5215 32 1481 32 4618
Fragments arranged by size:
5215 4618 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2622 5885 6162 1613
Size: 1009 3263 277 6861
Fragments arranged by size:
6861 3263 1009 277
PsiI TTA'TAA
Cuts at: 8980 8980
Size: 11410
PspOMI G'GGCC_C
Cuts at: 2734 5296 5694 8218 2734
Size: 2562 398 2524 5926
Fragments arranged by size:
5926 2562 2524 398
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4959 4991 4959
Size: 32 11378
PstI C_TGCA'G
Cuts at: 830 2121 2925 4267 4305 4434 5646 5906 6000
Size: 1291 804 1342 38 129 1212 260 94
Cuts at: 6000 6825 7614 7671 7809 8762 830
Size: 825 789 57 138 953 3478
Fragments arranged by size:
3478 1342 1291 1212 953 825 804 789
260 138 129 94 57 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11410
PvuII CAG'CTG
Cuts at: 2578 2827 2953 3674 4378 5363 5458 5995 6296
Size: 249 126 721 704 985 95 537 301
Cuts at: 6296 6349 6434 6803 7079 7495 7615 7783 8053
Size: 53 85 369 276 416 120 168 270
Cuts at: 8053 8188 2578
Size: 135 5800
Fragments arranged by size:
5800 985 721 704 537 416 369 301
276 270 249 168 135 126 120 95
85 53
SacI G_AGCT'C
Cuts at: 4806 6309 7937 4806
Size: 1503 1628 8279
Fragments arranged by size:
8279 1628 1503
SalI G'TCGA_C
Cuts at: 1936 5133 8752 1936
Size: 3197 3619 4594
Fragments arranged by size:
4594 3619 3197
SapI GCTCTTCn'nnn_
Cuts at: 5921 5921
Size: 11410
SbfI CC_TGCA'GG
Cuts at: 4305 8762 4305
Size: 4457 6953
ScaI AGT'ACT
Cuts at: 6905 7920 9658 11370 6905
Size: 1015 1738 1712 6945
Fragments arranged by size:
6945 1738 1712 1015
SexAI A'CCwGG_T
Cuts at: 2835 5533 8430 2835
Size: 2698 2897 5815
Fragments arranged by size:
5815 2897 2698
SfcI C'TryA_G
Cuts at: 826 1045 2117 2300 2921 3512 4061 4263 4301
Size: 219 1072 183 621 591 549 202 38
Cuts at: 4301 4430 4727 5642 5902 5996 6821 7610 7667
Size: 129 297 915 260 94 825 789 57
Cuts at: 7667 7805 7819 8758 9893 10574 10765 826
Size: 138 14 939 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 939 915 825 789 681
621 591 549 297 260 219 202 191
183 138 129 94 57 38 14
SfoI GGC'GCC
Cuts at: 3453 6119 7760 3453
Size: 2666 1641 7103
Fragments arranged by size:
7103 2666 1641
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11410
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11410
SmaI CCC'GGG
Cuts at: 1872 8737 1872
Size: 6865 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 2441 3071 5048 6096 7205
Size: 197 926 231 267 630 1977 1048 1109
Cuts at: 7205 7406 9514 10415 10677 820
Size: 201 2108 901 262 1553
Fragments arranged by size:
2108 1977 1553 1109 1048 926 901 630
267 262 231 201 197
SnaBI TAC'GTA
Cuts at: 493 9201 493
Size: 8708 2702
SpeI A'CTAG_T
Cuts at: 152 6527 152
Size: 6375 5035
SphI G_CATG'C
Cuts at: 1132 6901 8768 1132
Size: 5769 1867 3774
Fragments arranged by size:
5769 3774 1867
SspI AAT'ATT
Cuts at: 5 52 5274 9334 5
Size: 47 5222 4060 2081
Fragments arranged by size:
5222 4060 2081 47
StuI AGG'CCT
Cuts at: 1517 5896 1517
Size: 4379 7031
StyI C'CwwG_G
Cuts at: 513 1065 3193 3327 3768 4948 5413 5656 5721
Size: 552 2128 134 441 1180 465 243 65
Cuts at: 5721 6228 8487 8849 513
Size: 507 2259 362 3074
Fragments arranged by size:
3074 2259 2128 1180 552 507 465 441
362 243 134 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3474 3478 3566 4891 6208 9437 9454
Size: 1255 1894 4 88 1325 1317 3229 17
Cuts at: 9454 9607 9792 10237 325
Size: 153 185 445 1498
Fragments arranged by size:
3229 1894 1498 1325 1317 1255 445 185
153 88 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5280 6436 6903
Size: 274 80 33 51 1443 3303 1156 467
Cuts at: 6903 7137 7918 9656 11368 96
Size: 234 781 1738 1712 138
Fragments arranged by size:
3303 1738 1712 1443 1156 781 467 274
234 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3363 3395 3582 3614 3971 4003 4078 4110 5455
Size: 32 187 32 357 32 75 32 1345
Cuts at: 5455 5487 5630 5662 8154 8186 11370 11402 3363
Size: 32 143 32 2492 32 3184 32 3371
Fragments arranged by size:
3371 3184 2492 1345 357 187 143 75
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2837 7328 1316
Size: 1521 4491 5398
Fragments arranged by size:
5398 4491 1521
XbaI T'CTAG_A
Cuts at: 4516 8746 4516
Size: 4230 7180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5382 7612 5382
Size: 2230 9180
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11410
XmaI C'CCGG_G
Cuts at: 1870 8735 1870
Size: 6865 4545
XmnI GAAnn'nnTTC
Cuts at: 2390 4138 4418 4974 7857 9539 2390
Size: 1748 280 556 2883 1682 4261
Fragments arranged by size:
4261 2883 1748 1682 556 280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3828 9218 276
Size: 53 83 186 936 2294 5390 2468
Fragments arranged by size:
5390 2468 2294 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I FseI FspAI NruI
PacI PmlI PpuMI PspXI RsrII SacII SanDI SfiI
SrfI SwaI