Restriction map for
pFN21AA0099
(Circular) (Six-base) MAPSORT of: pFN21AA0099.seq Check: 2199 from: 1 to: 8191
pFN21AA0099 8191 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2880 6001 278
Size: 53 83 186 936 1344 3121 2468
Fragments arranged by size:
3121 2468 1344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3411 5534 1937
Size: 1474 2123 4594
Fragments arranged by size:
4594 2123 1474
Acc65I G'GTAC_C
Cuts at: 3103 4446 5512 3103
Size: 1343 1066 5782
Fragments arranged by size:
5782 1343 1066
AclI AA'CG_TT
Cuts at: 1563 6318 6691 1563
Size: 4755 373 3063
Fragments arranged by size:
4755 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3062 4320 4324 4749 6254 7638 7906 1632
Size: 1430 1258 4 425 1505 1384 268 1917
Fragments arranged by size:
1917 1505 1430 1384 1258 425 268 4
AfeI AGC'GCT
Cuts at: 3304 3304
Size: 8191
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7994
AflIII A'CryG_T
Cuts at: 1203 3058 5074 7090 8067 1203
Size: 1855 2016 2016 977 1327
Fragments arranged by size:
2016 2016 1855 1327 977
AgeI A'CCGG_T
Cuts at: 4443 7022 4443
Size: 2579 5612
AhdI GACnn_n'nnGTC
Cuts at: 6920 6920
Size: 8191
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3240 3456 5178 1184
Size: 2056 216 1722 4197
Fragments arranged by size:
4197 2056 1722 216
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3726 3758 3726
Size: 32 8159
AlwNI CAG_nnn'CTG
Cuts at: 2532 3128 4298 4669 5934 7506 2532
Size: 596 1170 371 1265 1572 3217
Fragments arranged by size:
3217 1572 1265 1170 596 371
ApaI G_GGCC'C
Cuts at: 5463 5463
Size: 8191
ApaLI G'TGCA_C
Cuts at: 5236 6248 7404 5236
Size: 1012 1156 6023
Fragments arranged by size:
6023 1156 1012
ApoI r'AATT_y
Cuts at: 1493 3607 4490 4646 5159 5500 5726 5878 7987
Size: 2114 883 156 513 341 226 152 2109
Cuts at: 7987 1493
Size: 1697
Fragments arranged by size:
2114 2109 1697 883 513 341 226 156
152
AseI AT'TA_AT
Cuts at: 160 1032 4782 6745 160
Size: 872 3750 1963 1606
Fragments arranged by size:
3750 1963 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8191
AvaI C'yCGr_G
Cuts at: 1870 1943 2692 4345 5516 1870
Size: 73 749 1653 1171 4545
Fragments arranged by size:
4545 1653 1171 749 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3087 3120 3087
Size: 33 8158
BamHI G'GATC_C
Cuts at: 3580 3983 4226 5521 5888 3580
Size: 403 243 1295 367 5883
Fragments arranged by size:
5883 1295 403 367 243
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2993 3103 3695 3971 4446
Size: 325 217 627 1206 110 592 276 475
Cuts at: 4446 5512 6967 618
Size: 1066 1455 1842
Fragments arranged by size:
1842 1455 1206 1066 627 592 475 325
276 217 110
BanII G_rGCy'C
Cuts at: 1391 2687 3739 3920 5463 5510 1391
Size: 1296 1052 181 1543 47 4072
Fragments arranged by size:
4072 1543 1296 1052 181 47
BbeI G_GCGC'C
Cuts at: 3975 3975
Size: 8191
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2587 4561 928
Size: 971 688 1974 4558
Fragments arranged by size:
4558 1974 971 688
BbvCI CC'TCA_GC
Cuts at: 3781 3810 3781
Size: 29 8162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 6382 6416 1903
Size: 34 2012 34 2399 34 3678
Fragments arranged by size:
3678 2399 2012 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6083 7293 6083
Size: 1210 6981
BclI T'GATC_A
Cuts at: 1548 2512 1548
Size: 964 7227
BfrBI ATG'CAT
Cuts at: 4863 5356 4863
Size: 493 7698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6802 136
Size: 107 122 71 631 605 5130 1525
Fragments arranged by size:
5130 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8186 8186
Size: 8191
BlpI GC'TnA_GC
Cuts at: 2968 3210 3495 4554 5608 2968
Size: 242 285 1059 1054 5551
Fragments arranged by size:
5551 1059 1054 285 242
Bme1580I G_kGCm'C
Cuts at: 1790 3700 5240 5463 6252 7408 1790
Size: 1910 1540 223 789 1156 2573
Fragments arranged by size:
2573 1910 1540 1156 789 223
BmgBI CAC'GTC
Cuts at: 1318 4699 5323 1318
Size: 3381 624 4186
Fragments arranged by size:
4186 3381 624
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2349 3758 4876
Size: 416 53 83 399 24 932 1409 1118
Cuts at: 4876 5922 6880 442
Size: 1046 958 1753
Fragments arranged by size:
1753 1409 1118 1046 958 932 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8191
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2456 3031 4371 2456
Size: 575 1340 6276
Fragments arranged by size:
6276 1340 575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2173 2435 4275 4315 4556 4568 4994 5102
Size: 934 262 1840 40 241 12 426 108
Cuts at: 5102 5444 6851 1239
Size: 342 1407 2579
Fragments arranged by size:
2579 1840 1407 934 426 342 262 241
108 40 12
Bpu10I CC'TnA_GC
Cuts at: 3781 3810 4130 3781
Size: 29 320 7842
Fragments arranged by size:
7842 320 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2067 4772 6316 7181 7479 2067
Size: 2705 1544 865 298 2779
Fragments arranged by size:
2779 2705 1544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6854 882
Size: 617 5355 2219
Fragments arranged by size:
5355 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4849 5204 5302 5982 493
Size: 711 3645 355 98 680 2702
Fragments arranged by size:
3645 2702 711 680 355 98
BsaBI GATnn'nnATC
Cuts at: 8185 8185
Size: 8191
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2877 3972 5998 6380
Size: 53 83 186 936 1344 1095 2026 382
Cuts at: 6380 7015 275
Size: 635 1451
Fragments arranged by size:
2026 1451 1344 1095 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3658 4443 6624 7022 7296 7443 7854 3658
Size: 785 2181 398 274 147 411 3995
Fragments arranged by size:
3995 2181 785 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2663 2693 3726 3756 4189
Size: 30 165 30 1260 30 1033 30 433
Cuts at: 4189 4219 1178
Size: 30 5150
Fragments arranged by size:
5150 1260 1033 433 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2304 2316 2831 3540 4132 5027 1186
Size: 1118 12 515 709 592 895 4350
Fragments arranged by size:
4350 1118 895 709 592 515 12
BseYI C'CCAG_C
Cuts at: 2177 2559 2973 3364 3816 3822 3924 7394 7890
Size: 382 414 391 452 6 102 3470 496
Cuts at: 7890 2177
Size: 2478
Fragments arranged by size:
3470 2478 496 452 414 391 382 102
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2594 2876 3771 1163
Size: 573 858 282 895 5583
Fragments arranged by size:
5583 895 858 573 282
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2639 5092 5641 6402 7430 1540
Size: 275 186 638 2453 549 761 1028 2301
Fragments arranged by size:
2453 2301 1028 761 638 549 275 186
BsiHKAI G_wGCw'C
Cuts at: 2687 3739 4980 5088 5223 5240 6252 6337 7408
Size: 1052 1241 108 135 17 1012 85 1071
Cuts at: 7408 2687
Size: 3470
Fragments arranged by size:
3470 1241 1071 1052 1012 135 108 85
17
BsiWI C'GTAC_G
Cuts at: 5204 5204
Size: 8191
BsmI GAATG_Cn'
Cuts at: 3715 5702 5795 3715
Size: 1987 93 6111
Fragments arranged by size:
6111 1987 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2687 3700 3739 3920 4980 5088 5223
Size: 399 897 1013 39 181 1060 108 135
Cuts at: 5223 5240 5463 5510 6252 6337 7408 1391
Size: 17 223 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 1060 1013 897 742 399 223
181 135 108 85 47 39 17
BspEI T'CCGG_A
Cuts at: 7854 7854
Size: 8191
BspHI T'CATG_A
Cuts at: 2392 6080 7810 2392
Size: 3688 1730 2773
Fragments arranged by size:
3688 2773 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3091 3248 3502 4765 5546 844
Size: 2247 157 254 1263 781 3489
Fragments arranged by size:
3489 2247 1263 781 254 157
BsrBI CCG'CTC
Cuts at: 1394 1823 6078 1394
Size: 429 4255 3507
Fragments arranged by size:
4255 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5140 5176 6686 6860 65
Size: 1183 3892 36 1510 174 1396
Fragments arranged by size:
3892 1510 1396 1183 174 36
BsrFI r'CCGG_y
Cuts at: 1700 1810 4443 6835 7022 1700
Size: 110 2633 2392 187 2869
Fragments arranged by size:
2869 2633 2392 187 110
BsrGI T'GTAC_A
Cuts at: 96 4873 5041 96
Size: 4777 168 3246
Fragments arranged by size:
4777 3246 168
BssHII G'CGCG_C
Cuts at: 1925 5212 1925
Size: 3287 4904
BssSI C'ACGA_G
Cuts at: 6251 7263 6251
Size: 1012 7179
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2443 794
Size: 1649 6542
BstBI TT'CG_AA
Cuts at: 4435 6995 4435
Size: 2560 5631
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8191
BstXI CCAn_nnnn'nTGG
Cuts at: 3004 3389 5302 3004
Size: 385 1913 5893
Fragments arranged by size:
5893 1913 385
BstYI r'GATC_y
Cuts at: 1972 2204 2932 3580 3983 4226 5361 5521 5888
Size: 232 728 648 403 243 1135 160 367
Cuts at: 5888 6273 6290 7742 7828 7840 8186 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1135 728 648 403 385 367
346 243 232 160 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5097 5097
Size: 8191
BtgI C'CryG_G
Cuts at: 513 1065 2376 3051 3520 4155 513
Size: 552 1311 675 469 635 4549
Fragments arranged by size:
4549 1311 675 635 552 469
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2441 3238 5359 487
Size: 683 56 1215 797 2121 3319
Fragments arranged by size:
3319 2121 1215 797 683 56
BtsI GCAGTG_nn'
Cuts at: 2043 3609 4039 4072 4273 4321 5707 6501 6521
Size: 1566 430 33 201 48 1386 794 20
Cuts at: 6521 2043
Size: 3713
Fragments arranged by size:
3713 1566 1386 794 430 201 48 33
20
ClaI AT'CG_AT
Cuts at: 1554 2329 4902 5226 1554
Size: 775 2573 324 4519
Fragments arranged by size:
4519 2573 775 324
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8156
DraI TTT'AAA
Cuts at: 5495 5495
Size: 8191
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7198 809
Size: 507 5882 1802
Fragments arranged by size:
5882 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4807 5415 5638
Size: 54 1425 50 275 18 2977 608 223
Cuts at: 5638 6527 8156 8
Size: 889 1629 43
Fragments arranged by size:
2977 1629 1425 889 608 275 223 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5638 1537
Size: 275 3826 4090
Fragments arranged by size:
4090 3826 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2416 3081 3911 4222 4237 4343 4369
Size: 668 395 665 830 311 15 106 26
Cuts at: 4369 5388 6121 1353
Size: 1019 733 3423
Fragments arranged by size:
3423 1019 830 733 668 665 395 311
106 26 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3976 5342 6778 7152 7298 387
Size: 1064 138 2387 1366 1436 374 146 1280
Fragments arranged by size:
2387 1436 1366 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2685 3737 2685
Size: 1052 7139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2435 3062 4275 4315 4320 4324
Size: 393 541 262 627 1213 40 5 4
Cuts at: 4324 4556 4568 4749 4994 5102 5444 6254 6851
Size: 232 12 181 245 108 342 810 597
Cuts at: 6851 7638 7906 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1213 810 787 627 597 541 393
342 268 262 245 232 181 108 40
12 5 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2199 2199
Size: 8191
EcoO109I rG'GnC_Cy
Cuts at: 5459 5459
Size: 8191
EcoRI G'AATT_C
Cuts at: 3607 5500 5878 3607
Size: 1893 378 5920
Fragments arranged by size:
5920 1893 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2057 2951 2057
Size: 894 7297
FspI TGC'GCA
Cuts at: 6697 6697
Size: 8191
HaeII r_GCGC'y
Cuts at: 1678 3306 3975 4299 4329 4561 7338 1678
Size: 1628 669 324 30 232 2777 2531
Fragments arranged by size:
2777 2531 1628 669 324 232 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3193 3225 3712 3744 4189
Size: 32 135 32 1621 32 487 32 445
Cuts at: 4189 4221 4683 4715 6832 6864 6906 6938 1373
Size: 32 462 32 2117 32 42 32 2626
Fragments arranged by size:
2626 2117 1621 487 462 445 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 5452 5535 5781 669
Size: 988 281 1263 2251 83 246 3079
Fragments arranged by size:
3079 2251 1263 988 281 246 83
HindIII A'AGCT_T
Cuts at: 748 4237 748
Size: 3489 4702
HpaI GTT'AAC
Cuts at: 5781 5781
Size: 8191
KasI G'GCGC_C
Cuts at: 3971 3971
Size: 8191
KpnI G_GTAC'C
Cuts at: 3107 4450 5516 3107
Size: 1343 1066 5782
Fragments arranged by size:
5782 1343 1066
MfeI C'AATT_G
Cuts at: 5790 5790
Size: 8191
MluI A'CGCG_T
Cuts at: 8067 8067
Size: 8191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3042 4047 5095 7305 7489 1305
Size: 1737 1005 1048 2210 184 2007
Fragments arranged by size:
2210 2007 1737 1048 1005 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4809 5417 10
Size: 54 1425 343 2977 608 2784
Fragments arranged by size:
2977 2784 1425 608 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4944 5276 6149 6508 6667 518
Size: 648 3778 332 873 359 159 2042
Fragments arranged by size:
3778 2042 873 648 359 332 159
MspA1I CmG'CkG
Cuts at: 1606 2156 2177 2977 3163 3206 3236 3272 3335
Size: 550 21 800 186 43 30 36 63
Cuts at: 3335 3368 3446 3452 3596 3785 4528 4553 5341
Size: 33 78 6 144 189 743 25 788
Cuts at: 5341 5607 6284 7432 7677 1606
Size: 266 677 1148 245 2120
Fragments arranged by size:
2120 1148 800 788 743 677 550 266
245 189 186 144 78 63 43 36
33 30 25 21 6
NarI GG'CG_CC
Cuts at: 3972 3972
Size: 8191
NcoI C'CATG_G
Cuts at: 513 1065 2376 3051 513
Size: 552 1311 675 5653
Fragments arranged by size:
5653 1311 675 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8191
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8191
NotI GC'GGCC_GC
Cuts at: 5638 5638
Size: 8191
NsiI A_TGCA'T
Cuts at: 4865 5358 4865
Size: 493 7698
NspI r_CATG'y
Cuts at: 1132 1229 2019 2107 3062 3649 4165 5078 5549
Size: 97 790 88 955 587 516 913 471
Cuts at: 5549 7094 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 955 913 790 587 516 471
97 88
PasI CC'CwG_GG
Cuts at: 1348 3314 3402 3913 3979 1348
Size: 1966 88 511 66 5560
Fragments arranged by size:
5560 1966 511 88 66
PciI A'CATG_T
Cuts at: 3058 5074 7090 3058
Size: 2016 2016 4159
Fragments arranged by size:
4159 2016 2016
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8191
PfoI T'CCnGG_A
Cuts at: 4147 4971 4147
Size: 824 7367
PmeI GTTT'AAAC
Cuts at: 5495 5495
Size: 8191
PmlI CAC'GTG
Cuts at: 4849 4849
Size: 8191
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3726 3758 6260 6292 7773 7805 3726
Size: 32 2502 32 1481 32 4112
Fragments arranged by size:
4112 2502 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8191
PsiI TTA'TAA
Cuts at: 4077 4122 5761 4077
Size: 45 1639 6507
Fragments arranged by size:
6507 1639 45
PspOMI G'GGCC_C
Cuts at: 5459 5459
Size: 8191
PstI C_TGCA'G
Cuts at: 830 3349 3457 3601 5543 830
Size: 2519 108 144 1942 3478
Fragments arranged by size:
3478 2519 1942 144 108
PvuI CG_AT'CG
Cuts at: 2001 2639 2001
Size: 638 7553
PvuII CAG'CTG
Cuts at: 2156 2177 2977 3163 3206 3236 3335 3368 3596
Size: 21 800 186 43 30 99 33 228
Cuts at: 3596 3785 4528 4553 2156
Size: 189 743 25 5794
Fragments arranged by size:
5794 800 743 228 189 186 99 43
33 30 25 21
SacI G_AGCT'C
Cuts at: 2687 3739 2687
Size: 1052 7139
SalI G'TCGA_C
Cuts at: 1936 5533 1936
Size: 3597 4594
SapI GCTCTTCn'nnn_
Cuts at: 2416 2416
Size: 8191
SbfI CC_TGCA'GG
Cuts at: 5543 5543
Size: 8191
ScaI AGT'ACT
Cuts at: 5432 6439 8151 5432
Size: 1007 1712 5472
Fragments arranged by size:
5472 1712 1007
SexAI A'CCwGG_T
Cuts at: 2584 2584
Size: 8191
SfcI C'TryA_G
Cuts at: 826 1045 2208 3345 3453 3597 4715 5539 6674
Size: 219 1163 1137 108 144 1118 824 1135
Cuts at: 6674 7355 7546 826
Size: 681 191 1471
Fragments arranged by size:
1471 1163 1137 1135 1118 824 681 219
191 144 108
SfoI GGC'GCC
Cuts at: 3973 3973
Size: 8191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8191
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8191
SmaI CCC'GGG
Cuts at: 1872 2694 5518 1872
Size: 822 2824 4545
Fragments arranged by size:
4545 2824 822
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2082 4751 6295 7196 7458 820
Size: 197 926 139 2669 1544 901 262 1553
Fragments arranged by size:
2669 1553 1544 926 901 262 197 139
SnaBI TAC'GTA
Cuts at: 493 5982 493
Size: 5489 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8191
SphI G_CATG'C
Cuts at: 1132 3649 4165 5549 1132
Size: 2517 516 1384 3774
Fragments arranged by size:
3774 2517 1384 516
SspI AAT'ATT
Cuts at: 5 52 2502 6115 5
Size: 47 2450 3613 2081
Fragments arranged by size:
3613 2450 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8191
StyI C'CwwG_G
Cuts at: 513 1065 2376 2729 3042 3051 3942 3991 5630
Size: 552 1311 353 313 9 891 49 1639
Cuts at: 5630 513
Size: 3074
Fragments arranged by size:
3074 1639 1311 891 552 353 313 49
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2498 6218 6235 6388 6573 7018 325
Size: 1255 918 3720 17 153 185 445 1498
Fragments arranged by size:
3720 1498 1255 918 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2623 4873 5041
Size: 274 80 33 51 1443 646 2250 168
Cuts at: 5041 5077 5137 5391 5430 6437 8149 96
Size: 36 60 254 39 1007 1712 138
Fragments arranged by size:
2250 1712 1443 1007 646 274 254 168
138 80 60 51 39 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8151 8183 8151
Size: 32 8159
Tth111I GACn'n_nGTC
Cuts at: 1316 2586 3630 1316
Size: 1270 1044 5877
Fragments arranged by size:
5877 1270 1044
XbaI T'CTAG_A
Cuts at: 5527 5527
Size: 8191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3058 3841 3940 4505 3058
Size: 783 99 565 6744
Fragments arranged by size:
6744 783 565 99
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8191
XmaI C'CCGG_G
Cuts at: 1870 2692 5516 1870
Size: 822 2824 4545
Fragments arranged by size:
4545 2824 822
XmnI GAAnn'nnTTC
Cuts at: 4431 4686 6320 4431
Size: 255 1634 6302
Fragments arranged by size:
6302 1634 255
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2878 5999 276
Size: 53 83 186 936 1344 3121 2468
Fragments arranged by size:
3121 2468 1344 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BsmBI BstZ17I DraIII EcoRV FseI
FspAI NaeI NgoMIV NruI PacI PpuMI PspXI PsrI
RsrII SacII SanDI SfiI SrfI SwaI