Restriction map for
pFN21AA0095
(Circular) (Six-base) MAPSORT of: pFN21AA0095.seq Check: 8693 from: 1 to: 7162
pFN21AA0095 7162 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2613 2613
Size: 7162
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3201 4972 278
Size: 53 83 186 936 1665 1771 2468
Fragments arranged by size:
2468 1771 1665 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4505 1937
Size: 2568 4594
Acc65I G'GTAC_C
Cuts at: 4483 4483
Size: 7162
AclI AA'CG_TT
Cuts at: 1563 5289 5662 1563
Size: 3726 373 3063
Fragments arranged by size:
3726 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2018 2330 2750 3272 3521 4013 5225 6609
Size: 386 312 420 522 249 492 1212 1384
Cuts at: 6609 6877 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1212 522 492 420 386 312
268 249
AfeI AGC'GCT
Cuts at: 4151 4151
Size: 7162
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6965
AflIII A'CryG_T
Cuts at: 1203 2670 2700 3696 6061 7038 1203
Size: 1467 30 996 2365 977 1327
Fragments arranged by size:
2365 1467 1327 996 977 30
AgeI A'CCGG_T
Cuts at: 5993 5993
Size: 7162
AhdI GACnn_n'nnGTC
Cuts at: 5891 5891
Size: 7162
AleI CACnn'nnGTG
Cuts at: 1166 2816 1166
Size: 1650 5512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7162
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4052 4084 4052
Size: 32 7130
AlwNI CAG_nnn'CTG
Cuts at: 4905 6477 4905
Size: 1572 5590
ApaI G_GGCC'C
Cuts at: 3139 3139
Size: 7162
ApaLI G'TGCA_C
Cuts at: 5219 6375 5219
Size: 1156 6006
ApoI r'AATT_y
Cuts at: 1493 2433 2568 2833 4052 4210 4471 4697 4849
Size: 940 135 265 1219 158 261 226 152
Cuts at: 4849 6958 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1219 940 265 261 226 158
152 135
AseI AT'TA_AT
Cuts at: 160 1032 5716 160
Size: 872 4684 1606
Fragments arranged by size:
4684 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
AvaI C'yCGr_G
Cuts at: 1870 1943 2195 2202 4487 1870
Size: 73 252 7 2285 4545
Fragments arranged by size:
4545 2285 252 73 7
BamHI G'GATC_C
Cuts at: 4492 4859 4492
Size: 367 6795
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2860 3284 4162 4483 5938
Size: 325 217 627 1073 424 878 321 1455
Cuts at: 5938 618
Size: 1842
Fragments arranged by size:
1842 1455 1073 878 627 424 325 321
217
BanII G_rGCy'C
Cuts at: 1391 2031 3028 3139 3947 4033 4481 1391
Size: 640 997 111 808 86 448 4072
Fragments arranged by size:
4072 997 808 640 448 111 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2469 3348 928
Size: 971 570 879 4742
Fragments arranged by size:
4742 971 879 570
BbvCI CC'TCA_GC
Cuts at: 2379 3542 2379
Size: 1163 5999
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4420 4454 5353 5387 1903
Size: 34 2483 34 899 34 3678
Fragments arranged by size:
3678 2483 899 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2002 5054 6264 2002
Size: 3052 1210 2900
Fragments arranged by size:
3052 2900 1210
BclI T'GATC_A
Cuts at: 1548 4089 1548
Size: 2541 4621
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5773 136
Size: 107 122 71 631 605 4101 1525
Fragments arranged by size:
4101 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7157 7157
Size: 7162
BlpI GC'TnA_GC
Cuts at: 3534 3941 4579 3534
Size: 407 638 6117
Fragments arranged by size:
6117 638 407
Bme1580I G_kGCm'C
Cuts at: 1790 3139 3970 4167 5223 6379 1790
Size: 1349 831 197 1056 1156 2573
Fragments arranged by size:
2573 1349 1156 1056 831 197
BmgBI CAC'GTC
Cuts at: 1318 2157 2701 1318
Size: 839 544 5779
Fragments arranged by size:
5779 839 544
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4893 5851 442
Size: 416 53 83 399 24 3476 958 1753
Fragments arranged by size:
3476 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7162
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 7162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3632 3810 3941 5822 1239
Size: 2393 178 131 1881 2579
Fragments arranged by size:
2579 2393 1881 178 131
Bpu10I CC'TnA_GC
Cuts at: 2379 3542 2379
Size: 1163 5999
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2466 2810 4017 4277 5287 6152 6450 2294
Size: 172 344 1207 260 1010 865 298 3006
Fragments arranged by size:
3006 1207 1010 865 344 298 260 172
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2205 2985 5825 882
Size: 617 706 780 2840 2219
Fragments arranged by size:
2840 2219 780 706 617
BsaAI yAC'GTr
Cuts at: 493 1204 4953 493
Size: 711 3749 2702
Fragments arranged by size:
3749 2702 711
BsaBI GATnn'nnATC
Cuts at: 7156 7156
Size: 7162
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2183 2466 3198 4969
Size: 53 83 186 936 650 283 732 1771
Cuts at: 4969 5351 5986 275
Size: 382 635 1451
Fragments arranged by size:
1771 1451 936 732 650 635 382 283
186 83 53
BsaWI w'CCGG_w
Cuts at: 5595 5993 6267 6414 6825 5595
Size: 398 274 147 411 5932
Fragments arranged by size:
5932 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2387 2417 1178
Size: 30 165 30 984 30 5923
Fragments arranged by size:
5923 984 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 2396 3530 3587 1186
Size: 997 213 1134 57 4761
Fragments arranged by size:
4761 1134 997 213 57
BseYI C'CCAG_C
Cuts at: 2229 2847 2851 3027 3037 3097 4326 6365 6861
Size: 618 4 176 10 60 1229 2039 496
Cuts at: 6861 2229
Size: 2530
Fragments arranged by size:
2530 2039 1229 618 496 176 60 10
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2271 2423 4460 1163
Size: 573 535 152 2037 3865
Fragments arranged by size:
3865 2037 573 535 152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4612 5373 6401 1540
Size: 275 186 2611 761 1028 2301
Fragments arranged by size:
2611 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 3535 5223 5308 6379 2031
Size: 1504 1688 85 1071 2814
Fragments arranged by size:
2814 1688 1504 1071 85
BsmI GAATG_Cn'
Cuts at: 3910 4673 4766 3910
Size: 763 93 6306
Fragments arranged by size:
6306 763 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2159 2159
Size: 7162
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 3028 3139 3535 3947 3970 4033
Size: 399 241 997 111 396 412 23 63
Cuts at: 4033 4167 4481 5223 5308 6379 1391
Size: 134 314 742 85 1071 2174
Fragments arranged by size:
2174 1071 997 742 412 399 396 314
241 134 111 85 63 23
BspEI T'CCGG_A
Cuts at: 6825 6825
Size: 7162
BspHI T'CATG_A
Cuts at: 4455 5051 6781 4455
Size: 596 1730 4836
Fragments arranged by size:
4836 1730 596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2613 3003 4517 844
Size: 1769 390 1514 3489
Fragments arranged by size:
3489 1769 1514 390
BsrBI CCG'CTC
Cuts at: 1394 1823 3454 5049 1394
Size: 429 1631 1595 3507
Fragments arranged by size:
3507 1631 1595 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4011 5657 5831 65
Size: 1183 2763 1646 174 1396
Fragments arranged by size:
2763 1646 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2713 5806 5993 1700
Size: 110 903 3093 187 2869
Fragments arranged by size:
3093 2869 903 187 110
BsrGI T'GTAC_A
Cuts at: 96 3606 96
Size: 3510 3652
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7162
BssSI C'ACGA_G
Cuts at: 3548 5222 6234 3548
Size: 1674 1012 4476
Fragments arranged by size:
4476 1674 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2446 2723 3431 794
Size: 1652 277 708 4525
Fragments arranged by size:
4525 1652 708 277
BstBI TT'CG_AA
Cuts at: 2884 5966 2884
Size: 3082 4080
BstEII G'GTnAC_C
Cuts at: 1184 3557 1184
Size: 2373 4789
BstXI CCAn_nnnn'nTGG
Cuts at: 3554 3554
Size: 7162
BstYI r'GATC_y
Cuts at: 1972 2109 3154 4492 4859 5244 5261 6713 6799
Size: 137 1045 1338 367 385 17 1452 86
Cuts at: 6799 6811 7157 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1338 1045 385 367 346 137
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3140 3665 3140
Size: 525 6637
BtgI C'CryG_G
Cuts at: 513 1065 2004 2679 3627 513
Size: 552 939 675 948 4048
Fragments arranged by size:
4048 948 939 675 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6423
Fragments arranged by size:
6423 683 56
BtsI GCAGTG_nn'
Cuts at: 2444 4678 5472 5492 2444
Size: 2234 794 20 4114
Fragments arranged by size:
4114 2234 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2245 2280 529
Size: 35 1681 35 5411
Fragments arranged by size:
5411 1681 35 35
DraI TTT'AAA
Cuts at: 3049 3513 4466 3049
Size: 464 953 5745
Fragments arranged by size:
5745 953 464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2523 6169 809
Size: 507 1207 3646 1802
Fragments arranged by size:
3646 1802 1207 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4609 5498 7127
Size: 54 1425 50 275 18 2779 889 1629
Cuts at: 7127 8
Size: 43
Fragments arranged by size:
2779 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4609 1537
Size: 275 2797 4090
Fragments arranged by size:
4090 2797 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2343 2354 3867 4182 5092 1353
Size: 990 11 1513 315 910 3423
Fragments arranged by size:
3423 1513 990 910 315 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3239 5749 6123 6269 387
Size: 1064 138 1650 2510 374 146 1280
Fragments arranged by size:
2510 1650 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2029 2029
Size: 7162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2018 2330 2750 3272 3521 3632 3810
Size: 393 386 312 420 522 249 111 178
Cuts at: 3810 3941 4013 5225 5822 6609 6877 1239
Size: 131 72 1212 597 787 268 1524
Fragments arranged by size:
1524 1212 787 597 522 420 393 386
312 268 249 178 131 111 72
EcoNI CCTnn'n_nnAGG
Cuts at: 4172 4172
Size: 7162
EcoO109I rG'GnC_Cy
Cuts at: 2364 2516 3135 3136 3643 4323 4450 2364
Size: 152 619 1 507 680 127 5076
Fragments arranged by size:
5076 680 619 507 152 127 1
EcoRI G'AATT_C
Cuts at: 2433 4471 4849 2433
Size: 2038 378 4746
Fragments arranged by size:
4746 2038 378
EcoRV GAT'ATC
Cuts at: 4141 4141
Size: 7162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2486 4268 2383
Size: 103 1782 5277
Fragments arranged by size:
5277 1782 103
FspI TGC'GCA
Cuts at: 2756 5668 2756
Size: 2912 4250
FspAI rTGC'GCAy
Cuts at: 2756 2756
Size: 7162
HaeII r_GCGC'y
Cuts at: 1678 2131 2966 4153 6309 1678
Size: 453 835 1187 2156 2531
Fragments arranged by size:
2531 2156 1187 835 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3183 3215 4334 4366 5803
Size: 32 135 32 1611 32 1119 32 1437
Cuts at: 5803 5835 5877 5909 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1611 1437 1119 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2709 4506 4752 669
Size: 988 281 771 1797 246 3079
Fragments arranged by size:
3079 1797 988 771 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7162
HpaI GTT'AAC
Cuts at: 4752 4752
Size: 7162
KpnI G_GTAC'C
Cuts at: 4487 4487
Size: 7162
MfeI C'AATT_G
Cuts at: 2876 4761 2876
Size: 1885 5277
MluI A'CGCG_T
Cuts at: 7038 7038
Size: 7162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2691 3318 4002 6276 6460 1305
Size: 1386 627 684 2274 184 2007
Fragments arranged by size:
2274 2007 1386 684 627 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5340
Fragments arranged by size:
5340 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2549 2816 5120 5479 5638 518
Size: 648 1383 267 2304 359 159 2042
Fragments arranged by size:
2304 2042 1383 648 359 267 159
MspA1I CmG'CkG
Cuts at: 1606 2851 3037 4578 5255 6403 6648 1606
Size: 1245 186 1541 677 1148 245 2120
Fragments arranged by size:
2120 1541 1245 1148 677 245 186
NcoI C'CATG_G
Cuts at: 513 1065 2004 2679 513
Size: 552 939 675 4996
Fragments arranged by size:
4996 939 675 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7162
NotI GC'GGCC_GC
Cuts at: 4609 4609
Size: 7162
NspI r_CATG'y
Cuts at: 1132 1229 2674 2983 3700 4520 6065 1132
Size: 97 1445 309 717 820 1545 2229
Fragments arranged by size:
2229 1545 1445 820 717 309 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7162
PciI A'CATG_T
Cuts at: 2670 3696 6061 2670
Size: 1026 2365 3771
Fragments arranged by size:
3771 2365 1026
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2236 1108
Size: 1128 6034
PfoI T'CCnGG_A
Cuts at: 2354 3060 2354
Size: 706 6456
PmeI GTTT'AAAC
Cuts at: 4466 4466
Size: 7162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5231 5263 6744 6776 5231
Size: 32 1481 32 5617
Fragments arranged by size:
5617 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2364 2516 4450 2364
Size: 152 1934 5076
Fragments arranged by size:
5076 1934 152
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7162
PsiI TTA'TAA
Cuts at: 2802 4732 2802
Size: 1930 5232
PspOMI G'GGCC_C
Cuts at: 3135 3135
Size: 7162
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2085 2117 2085
Size: 32 7130
PstI C_TGCA'G
Cuts at: 830 2610 4514 830
Size: 1780 1904 3478
Fragments arranged by size:
3478 1904 1780
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7162
PvuII CAG'CTG
Cuts at: 2851 3037 2851
Size: 186 6976
SacI G_AGCT'C
Cuts at: 2031 2031
Size: 7162
SalI G'TCGA_C
Cuts at: 1936 4504 1936
Size: 2568 4594
SanDI GG'GwC_CC
Cuts at: 2516 2516
Size: 7162
SbfI CC_TGCA'GG
Cuts at: 4514 4514
Size: 7162
ScaI AGT'ACT
Cuts at: 3653 5410 7122 3653
Size: 1757 1712 3693
Fragments arranged by size:
3693 1757 1712
SexAI A'CCwGG_T
Cuts at: 2616 3053 2616
Size: 437 6725
SfcI C'TryA_G
Cuts at: 826 1045 2606 2927 4510 5645 6326 6517 826
Size: 219 1561 321 1583 1135 681 191 1471
Fragments arranged by size:
1583 1561 1471 1135 681 321 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7162
SmaI CCC'GGG
Cuts at: 1872 4489 1872
Size: 2617 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2273 2481 2789 4032 4292 5266
Size: 197 926 330 208 308 1243 260 974
Cuts at: 5266 6167 6429 820
Size: 901 262 1553
Fragments arranged by size:
1553 1243 974 926 901 330 308 262
260 208 197
SnaBI TAC'GTA
Cuts at: 493 4953 493
Size: 4460 2702
SpeI A'CTAG_T
Cuts at: 152 3809 152
Size: 3657 3505
SphI G_CATG'C
Cuts at: 1132 2983 4520 1132
Size: 1851 1537 3774
Fragments arranged by size:
3774 1851 1537
SspI AAT'ATT
Cuts at: 5 52 5086 5
Size: 47 5034 2081
Fragments arranged by size:
5034 2081 47
StuI AGG'CCT
Cuts at: 1517 2777 1517
Size: 1260 5902
StyI C'CwwG_G
Cuts at: 513 1065 2004 2679 2778 3635 4601 513
Size: 552 939 675 99 857 966 3074
Fragments arranged by size:
3074 966 939 857 675 552 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5189 5206 5359 5544 5989 325
Size: 1255 3609 17 153 185 445 1498
Fragments arranged by size:
3609 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3066 3174 3606
Size: 274 80 33 51 1443 1089 108 432
Cuts at: 3606 3651 5408 7120 96
Size: 45 1757 1712 138
Fragments arranged by size:
1757 1712 1443 1089 432 274 138 108
80 51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3230 3262 7122 7154 3230
Size: 32 3860 32 3238
Fragments arranged by size:
3860 3238 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7162
XbaI T'CTAG_A
Cuts at: 4498 4498
Size: 7162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4432 4432
Size: 7162
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7162
XmaI C'CCGG_G
Cuts at: 1870 4487 1870
Size: 2617 4545
XmnI GAAnn'nnTTC
Cuts at: 4248 5291 4248
Size: 1043 6119
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3199 4970 276
Size: 53 83 186 936 1665 1771 2468
Fragments arranged by size:
2468 1771 1665 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI RsrII SacII SapI SfiI SfoI SrfI
SwaI